13 results about "Microbial ecology" patented technology

The invention relates to a compound soil improvement fertilizer and a preparation process thereof, and belongs to the technical field of agricultural environmental protection. The fertilizer is prepared from a modified biochar carrier, a compound microbial agent, an organic fermentation substrate and an inorganic mineral component. The modified charcoal carrier forms a hierarchical pore structure through acid modification and mineral loading; the compound microbial agent comprises phosphate solubilizing bacteria, nitrogen-fixing bacteria and organic matter degrading bacteria; the organic fermentation substrate is prepared from mushroom bran, activated sludge and corncob through segmented fermentation; the inorganic mineral component comprises attapulgite and dolomite powder. The preparation process comprises the steps of biochar modification treatment, microorganism composite culture, organic matrix segmented fermentation and slow-release coating granulation. The fertilizer can synchronously improve the physical structure, chemical properties and microbial ecology of soil, significantly improve the nutrient utilization efficiency, reduce the application amount of the chemical fertilizer, effectively repair degraded soil and enhance the stress resistance of crops.

This invention discloses a multi-scale prediction method and system for the maximum growth rate of soil microorganisms based on genomic features, involving the interdisciplinary fields of microbial ecology and genomics. The method includes: genomic data preparation, core genomic feature extraction, single-scale growth rate prediction, community-scale extrapolation, and result verification and output. The system includes a genomic data processing unit, a core feature extraction module, a growth rate prediction model, a community-scale extrapolation module, and a result visualization and verification unit. This invention overcomes the limitations of traditional culture methods and in-situ measurement methods, achieving high-throughput and accurate prediction of the growth rates of culturable and uncultured microorganisms, and solving the problems of low coverage, fragmented scales, and high costs of existing technologies. It is applicable to soil microbial function assessment, carbon cycle model parameterization, and ecosystem management.

The embodiment of the invention provides a soil bacterial community structure evaluation method and system based on an intermediate infrared spectrum transfer function and application, and relates to the technical field of soil microbial ecology. The method comprises the following steps: S1, collecting a soil sample; s2, acquiring a mid-infrared diffuse reflection spectrum of the soil sample to be detected; s3, acquiring an environment covariant corresponding to the soil sample, wherein the environment covariant comprises at least one of soil physicochemical properties, climate data and topographic parameters; s4, splicing the mid-infrared diffuse reflection spectrum obtained in the step S2 and the environment covariable obtained in the step S3 to form a fusion feature vector as an input feature set; and S5, inputting the input feature set into a pre-trained nonlinear machine learning regression model, and outputting the relative abundance and / or OTU richness of at least one bacteriophage in the soil sample. According to the method, a technical route of combining a mid-infrared (MIR) spectrum and a spectral transfer function (STF) is adopted and is used for predicting the soil bacterial community structure.

The invention provides a humus-aluminum oxide composite material, application thereof and a soda alkali soil improvement method, and relates to the technical field of saline-alkali soil improvement. The invention provides a humus-aluminum oxide composite material. The humus-aluminum oxide composite material comprises natural humus and aluminum oxide. The natural humus material and the aluminum oxide adopted by the invention respectively show obvious advantages in the aspects of improving the organic matter content, the structural stability, the salinity regulation and control, the microbial ecology and the like of the soil. The two components are jointly applied to the saline-alkali soil, the physical-chemical-biological improvement effects of the two components can be synergistically exerted, the soil quality and the ecological function are improved, and a new path and theoretical support are provided for efficient and sustainable utilization of the saline-alkali soil.

The invention belongs to the technical field of soil nitrogen fixation and environmental protection, and relates to a method for enhancing soil nitrogen fixation by using pure iron powder. A method for enhancing soil nitrogen fixation by using pure iron powder comprises the following steps: mixing rice soil and wheat straw powder to obtain a soil sample; the method comprises the following steps: mixing a soil sample with pure iron powder under a waterflooding condition; pure iron powder is added to regulate and control microbial ecology of rice soil biological nitrogen fixation, the soil iron reduction process is enhanced, the abundance of iron reducing bacteria in soil is improved, the relative contribution of iron reducing bacteria nitrogen fixation is enhanced to a certain extent, the diversity of iron reducing bacteria communities is reduced, the ecological niches of dominant groups are enhanced, and the ecological niches of the dominant groups are increased. Finally, the nitrogenase activity of the soil is enhanced, and the nitrogen fixation capacity of the rice soil is improved.

The invention is suitable for the cross technical field of agricultural environment microbial ecology and heavy metal pollution remediation, and provides a method for analyzing a rhizosphere microdomain microorganism driving mechanism for regulating cadmium accumulation of oilseed rape through a selenium fertilizer. And the physical separation of rhizosphere and non-rhizosphere soil is realized by synchronously adopting a special rhizosphere compartment micro-area device. By measuring the Cd / Se content of plants and soil, the rhizosphere microbial community structure, the functional gene (dsrA) abundance and microorganism-environmental factor correlation analysis, the system reveals that the selenium fertilizer (500-1000g Se / hm < 2 >) drives rhizosphere to enrich key functional microorganisms such as desulfothiones and geobacteria, and the sulfate reducing capacity of the selenium fertilizer (500-1000g Se / hm < 2 >) is remarkably improved (the abundance of the dsrA gene is increased by 250%); therefore, a microbial mechanism of cadmium bio-availability is reduced. The invention provides methodological and theoretical support for farmland heavy metal pollution remediation by utilizing microbial regulation and control.

The invention relates to a bottom mud ecological optimization-based intelligent cultivation method and device for buried habitat type shellfishes, and the method comprises the steps: S1, constructing a controllable ecological cultivation unit: laying a liftable cultivation frame or net cage in a cultivation water area as a cultivation unit, and filling the cultivation unit with bottom mud subjected to thermal refining treatment to form a mud filling layer for the shellfishes to be buried and habitat; and S2, implementing intelligent dynamic ecological regulation and control: in the breeding process, based on the environment parameters monitored in real time, executing the following coordinated regulation and control operations on the breeding units through an intelligent control system. According to the method, a stable, controllable and healthy burying environment is created for shellfish, and the survival rate and the growth speed are remarkably increased; by means of active regulation and control, good redox state and microbial ecology of the bottom mud are maintained, and aging of the bottom mud is delayed; controllable management and mechanical harvesting in the breeding process are achieved, the labor intensity is greatly reduced, and the method is suitable for a polyculture system, so that the ecological sustainability and economic benefits of the whole breeding system are improved.

This invention relates to the field of resource and environmental technology, specifically to a method and application of an algae-bacteria hybrid system for treating high ammonia nitrogen wastewater. By regulating nutrient and operational parameters, a functional community is constructed and maintained, dominated by microalgae and supplemented by bacteria and cyanobacteria, with their relative abundance within a preset optimized range. A clear interspecific interaction network forms within the system: microalgae such as *Nostoc* and *Chlorella* exhibit mutualistic symbiosis, while bacteria such as *Flavobacterium* show antagonistic relationships with these microalgae. Positive and negative feedback mechanisms maintain community balance and system robustness. The three components complement each other: microalgae primarily drive photocatalytic nitrogen and phosphorus removal, cyanobacteria provide nitrogen fixation and stability, and functional bacteria participate in organic matter degradation and nitrogen cycling, achieving synergistic metabolism through material exchange. Microbial ecological analysis confirms that a stable community structure and internal interaction mechanisms are the core of efficient and stable ammonia nitrogen reduction. This technology offers precise regulation, stable operation, and is environmentally friendly and economical, and can be widely applied to the treatment of various types of ammonia nitrogen-polluted water bodies.

The invention discloses a method for establishing microbial ecology in red mud. The method comprises the following steps: A, adding an organic neutralizer into the red mud, uniformly mixing, and enabling the pH value of the red mud to be 8.5-9.5; b, adding a carbon source substance, a nitrogen source substance, a structure improving substance and naturally collected soil into the red mud in the step A, uniformly mixing, standing and fermenting at 15-40 DEG C for not less than 7 days, and stirring once every 28 hours during the period; the pH value of the fermented red mud is 8.3-9.3, the content of microorganisms is not less than 1.005 * 10 < 5 > / g, and the content of organic matters is not less than 3%. By optimizing the raw material ratio and the fermentation process, a microenvironment suitable for growth of microorganisms is created, rich microbial communities are introduced and cultured, soil improvement of the red mud is achieved, and an efficient solution is provided for ecological restoration of the red mud storage yard.

This invention relates to the field of wastewater treatment technology, and in particular to a coupled control system and method for deep denitrification of wastewater, comprising a real-time water quality monitoring module, a microbial twin modeling module, a real-time prediction module, an intelligent decision-making module, an actuator module, and a predictive control module. Compared to existing technologies that typically employ passive feedback control strategies based on effluent water quality indicators, which suffer from strong lag and are unable to cope with drastic fluctuations in influent load, this invention elevates the control objective from traditional process parameter setpoints to direct optimization of microbial ecological functions by constructing a digital twin capable of reflecting the functional state of the microbial community in real time. This represents a fundamental leap from "controlling process parameters" to "regulating microbial ecology," enabling intervention at the root of the reaction process and giving the system proactive health management capabilities, thereby significantly improving the stability of treatment efficiency and the level of intelligence in handling complex operating conditions.

This invention discloses a temperature control system for aquaculture fermentation beds, belonging to the field of aquaculture fermentation bed temperature control technology. It includes a fermentation bed environment sensing module, a data processing intelligent decision-making module, a precise ventilation control module, an intelligent temperature control module, a humidity collaborative control module, a material turning module, and an anomaly early warning and emergency handling module. This invention constructs an intelligent decision-making system from passive response to active prediction by integrating a multi-parameter fusion algorithm, adaptive PID control, and an LSTM prediction model. Dynamic comprehensive index calculation makes the control strategy closer to the needs of microbial ecology. The improved PID algorithm achieves parameter self-tuning through gradient descent, significantly improving control accuracy and stability. The LSTM model predicts future environmental changes based on historical data, combined with a dynamic threshold configuration unit, enabling the system to automatically optimize control parameters according to the aquaculture stage and raw material ratio, achieving intelligent adaptation across scenarios.

The application discloses a device for measuring root-arbuscular mycorrhizal fungus interaction for reducing nitrous oxide emission, and relates to the technical field of soil ecology and microbial ecology test, and comprises three experimental components arranged in sequence, wherein the experimental components comprise: an experimental tank, a partition plate is arranged in the middle of the experimental tank, and the partition plate is used for separating the experimental tank into a planting area and a mycelium area; a collecting frame is partially embedded in the mycelium area, and a water tank is arranged around the collecting frame; a gas collecting top base is closed at the top and open at the bottom, and the open edge of the gas collecting top base is immersed in the water tank; an exhaust pipe assembly is arranged on the gas collecting top base and used for conveying the gas in the gas collecting top base to a collecting cylinder assembly; and a plurality of spray pipes are distributed at intervals on one side of the partition plate, and the spray pipes are provided with a spray head assembly which sprays towards the partition plate. The device discloses the influence of arbuscular mycorrhizal fungi on soil nitrous oxide emission through a control experiment.