25 results about "Microbial ecology" patented technology

The invention relates to a compound soil improvement fertilizer and a preparation process thereof, and belongs to the technical field of agricultural environmental protection. The fertilizer is prepared from a modified biochar carrier, a compound microbial agent, an organic fermentation substrate and an inorganic mineral component. The modified charcoal carrier forms a hierarchical pore structure through acid modification and mineral loading; the compound microbial agent comprises phosphate solubilizing bacteria, nitrogen-fixing bacteria and organic matter degrading bacteria; the organic fermentation substrate is prepared from mushroom bran, activated sludge and corncob through segmented fermentation; the inorganic mineral component comprises attapulgite and dolomite powder. The preparation process comprises the steps of biochar modification treatment, microorganism composite culture, organic matrix segmented fermentation and slow-release coating granulation. The fertilizer can synchronously improve the physical structure, chemical properties and microbial ecology of soil, significantly improve the nutrient utilization efficiency, reduce the application amount of the chemical fertilizer, effectively repair degraded soil and enhance the stress resistance of crops.

The invention discloses a feed additive for improving mutton quality and producing low-mutton-smell mutton, which is characterized in that through combined application of phytosterol and dl-alpha-tocopheryl acetate, the microbial composition of the lamb intestinal tract is improved, especially the abundance of beneficial bacteria is improved, and the abundance of potential pathogenic bacteria is reduced. The combined use of the two can improve the intestinal microbial ecology, and significantly reduce the drip loss, shear force and Plt of mutton; 0.05, the mutton color and the tenderness of the mutton are improved; excrement causing secondary pollution to the environment is not generated. The application of the plant extract in the production of the low-mutton-smell mutton is reported for the first time, the mutton smell can be remarkably reduced by combining and adding the phytosterol and the dl-alpha-tocopheryl acetate according to a certain proportion, and meanwhile the mutton quality is improved. The feed additive disclosed by the invention is simple in components, remarkable in effect and simple and convenient to apply, and has great potential popularization and application values.

The invention relates to a method for identifying microbial core species in a river ecosystem, and belongs to the field of microbial ecology. Through OTU relative abundance data, a presence-absence matrix is constructed, and an OTU occurrence rate and an average relative abundance are calculated. And evaluating the OTU ecological importance, and identifying a potential core OTU. And analyzing the consistency of the OTU occurrence frequency and the time scale in combination with the time point location, and generating a ranking index Index for ranking. And performing Bray-Curis similarity analysis to quantify the structural diversity of the OTU community, and identifying the OTU which has the greatest influence on the community similarity. And determining a core OTU threshold value based on an inflection point method and a growth rate method, verifying a core classification group combination mechanism by using a Sloan neutral model, and finally determining the representativeness of the core OTU by comparing the actual occurrence frequency with model prediction. According to the method, rich information is provided for explaining the diversity pattern of the microflora level.

This invention discloses a multi-scale prediction method and system for the maximum growth rate of soil microorganisms based on genomic features, involving the interdisciplinary fields of microbial ecology and genomics. The method includes: genomic data preparation, core genomic feature extraction, single-scale growth rate prediction, community-scale extrapolation, and result verification and output. The system includes a genomic data processing unit, a core feature extraction module, a growth rate prediction model, a community-scale extrapolation module, and a result visualization and verification unit. This invention overcomes the limitations of traditional culture methods and in-situ measurement methods, achieving high-throughput and accurate prediction of the growth rates of culturable and uncultured microorganisms, and solving the problems of low coverage, fragmented scales, and high costs of existing technologies. It is applicable to soil microbial function assessment, carbon cycle model parameterization, and ecosystem management.

The invention discloses a soil non-symbiotic nitrogen fixation microorganism detection method based on a 15N2 tracer method, and belongs to the technical field of biological nitrogen fixation and microbial ecology. The method comprises the following steps: (1) carrying out a 15N2 isotope microcosm experiment; (2) determination of gas and soil 15N abundance in a microcosm experiment; (3) separating stable isotope labeled DNA and non-labeled DNA; and (4) identifying the enrichment degree of the 15N-DNA and analyzing the flora structure. On the basis of a 15N2 tracer method, a stable isotope nucleic acid probe technology and a 15N2 isotope direct labeling technology are integrated, the active nitrogen-fixing microorganisms and the ecological functions of the active nitrogen-fixing microorganisms can be researched at the same time, and technical support is provided for defining the types of the soil active nitrogen-fixing microorganisms in a natural ecological system and making contribution to nitrogen input. The method is simple and efficient in operation process, and has the advantages of high sensitivity and rapidness.

The embodiment of the invention provides a soil bacterial community structure evaluation method and system based on an intermediate infrared spectrum transfer function and application, and relates to the technical field of soil microbial ecology. The method comprises the following steps: S1, collecting a soil sample; s2, acquiring a mid-infrared diffuse reflection spectrum of the soil sample to be detected; s3, acquiring an environment covariant corresponding to the soil sample, wherein the environment covariant comprises at least one of soil physicochemical properties, climate data and topographic parameters; s4, splicing the mid-infrared diffuse reflection spectrum obtained in the step S2 and the environment covariable obtained in the step S3 to form a fusion feature vector as an input feature set; and S5, inputting the input feature set into a pre-trained nonlinear machine learning regression model, and outputting the relative abundance and / or OTU richness of at least one bacteriophage in the soil sample. According to the method, a technical route of combining a mid-infrared (MIR) spectrum and a spectral transfer function (STF) is adopted and is used for predicting the soil bacterial community structure.

The invention provides a humus-aluminum oxide composite material, application thereof and a soda alkali soil improvement method, and relates to the technical field of saline-alkali soil improvement. The invention provides a humus-aluminum oxide composite material. The humus-aluminum oxide composite material comprises natural humus and aluminum oxide. The natural humus material and the aluminum oxide adopted by the invention respectively show obvious advantages in the aspects of improving the organic matter content, the structural stability, the salinity regulation and control, the microbial ecology and the like of the soil. The two components are jointly applied to the saline-alkali soil, the physical-chemical-biological improvement effects of the two components can be synergistically exerted, the soil quality and the ecological function are improved, and a new path and theoretical support are provided for efficient and sustainable utilization of the saline-alkali soil.

The invention belongs to the technical field of soil nitrogen fixation and environmental protection, and relates to a method for enhancing soil nitrogen fixation by using pure iron powder. A method for enhancing soil nitrogen fixation by using pure iron powder comprises the following steps: mixing rice soil and wheat straw powder to obtain a soil sample; the method comprises the following steps: mixing a soil sample with pure iron powder under a waterflooding condition; pure iron powder is added to regulate and control microbial ecology of rice soil biological nitrogen fixation, the soil iron reduction process is enhanced, the abundance of iron reducing bacteria in soil is improved, the relative contribution of iron reducing bacteria nitrogen fixation is enhanced to a certain extent, the diversity of iron reducing bacteria communities is reduced, the ecological niches of dominant groups are enhanced, and the ecological niches of the dominant groups are increased. Finally, the nitrogenase activity of the soil is enhanced, and the nitrogen fixation capacity of the rice soil is improved.

The invention is suitable for the cross technical field of agricultural environment microbial ecology and heavy metal pollution remediation, and provides a method for analyzing a rhizosphere microdomain microorganism driving mechanism for regulating cadmium accumulation of oilseed rape through a selenium fertilizer. And the physical separation of rhizosphere and non-rhizosphere soil is realized by synchronously adopting a special rhizosphere compartment micro-area device. By measuring the Cd / Se content of plants and soil, the rhizosphere microbial community structure, the functional gene (dsrA) abundance and microorganism-environmental factor correlation analysis, the system reveals that the selenium fertilizer (500-1000g Se / hm < 2 >) drives rhizosphere to enrich key functional microorganisms such as desulfothiones and geobacteria, and the sulfate reducing capacity of the selenium fertilizer (500-1000g Se / hm < 2 >) is remarkably improved (the abundance of the dsrA gene is increased by 250%); therefore, a microbial mechanism of cadmium bio-availability is reduced. The invention provides methodological and theoretical support for farmland heavy metal pollution remediation by utilizing microbial regulation and control.

The invention discloses an efficient and low-loss single cell extraction method in complex flora and application in vegetative mass-dormant mass separation and living cell detection, and belongs to the technical field of microbial ecology. According to the method, an efficient and low-loss Nycodenz-Percoll extraction strategy is developed, the problems of low extraction efficiency, flora extraction structure deviation and the like of a traditional method are solved, and the method can effectively distinguish vegetative bodies from dormant bodies. According to the method, the microbial flora can be efficiently and nondestructively extracted from a complex matrix, and live / dead staining reagents are integrated to identify the physiological status. Meanwhile, according to the method, Percoll density gradient centrifugation is further fused for testing, and secondary separation can be further carried out on part of dormant bodies and vegetative cells, so that the purpose of efficient and graded purification is achieved.

The invention discloses a device for directly measuring the dynamic influence of root and arbuscular mycorrhizal fungi on soil organic carbon, which relates to the technical field of soil ecology and microbial ecology tests, and comprises three separation pipes distributed at intervals in the circumferential direction and a filler supply assembly for supplying fillers to the separation pipes, the separation tube is buried in the soil on one side of the root and arbuscular mycorrhizal fungi to be measured, and comprises a bottom tube, a top tube and a bottom tube, the top pipe fixedly communicates with the top of the bottom pipe, at least one through groove is formed in the side portion of the top pipe, and a ring piece is fixed to the inner wall of the top pipe; the net cylinder is attached to the inner wall of the top pipe in a sliding mode and supported by a ring piece, a net body is arranged on the side portion of the net cylinder, and the net body covers the through groove; the compactness adjusting pipe is attached to the inner wall of the net cylinder in a sliding mode and used for being matched with the bottom pipe to receive the filler and adjusting the compactness of the filler, and the device can evaluate the dynamic influence of plant root systems and arbuscular mycorrhizal fungi on soil organic carbon.

The invention relates to the technical field of soil improvement, and discloses an ecological soil conditioner based on charcoal and a multi-bacteria composite system as a core. 160 to 220 parts of camellia peel; 80 to 150 parts of a mixed improved microbial agent; and 200 to 300 parts of wormcast. The biochar is derived from pyrolysis and carbonization of plant residues. The mixed improved fungicide is prepared by mixing and stirring trichoderma viride, bacillus subtilis and bacillus mucilaginosus according to a ratio of 1: 1: 1. Through the multi-layer synergistic effect of physics, chemistry and biology, the physical and chemical properties of soil, the microbial ecology and the growth environment of crops are effectively improved. The modifier has significant advantages in traditional chemical modifiers and single biological modifiers, and can achieve a multifunctional improvement effect for different soil problems.

The invention relates to a bottom mud ecological optimization-based intelligent cultivation method and device for buried habitat type shellfishes, and the method comprises the steps: S1, constructing a controllable ecological cultivation unit: laying a liftable cultivation frame or net cage in a cultivation water area as a cultivation unit, and filling the cultivation unit with bottom mud subjected to thermal refining treatment to form a mud filling layer for the shellfishes to be buried and habitat; and S2, implementing intelligent dynamic ecological regulation and control: in the breeding process, based on the environment parameters monitored in real time, executing the following coordinated regulation and control operations on the breeding units through an intelligent control system. According to the method, a stable, controllable and healthy burying environment is created for shellfish, and the survival rate and the growth speed are remarkably increased; by means of active regulation and control, good redox state and microbial ecology of the bottom mud are maintained, and aging of the bottom mud is delayed; controllable management and mechanical harvesting in the breeding process are achieved, the labor intensity is greatly reduced, and the method is suitable for a polyculture system, so that the ecological sustainability and economic benefits of the whole breeding system are improved.

This invention relates to the field of resource and environmental technology, specifically to a method and application of an algae-bacteria hybrid system for treating high ammonia nitrogen wastewater. By regulating nutrient and operational parameters, a functional community is constructed and maintained, dominated by microalgae and supplemented by bacteria and cyanobacteria, with their relative abundance within a preset optimized range. A clear interspecific interaction network forms within the system: microalgae such as *Nostoc* and *Chlorella* exhibit mutualistic symbiosis, while bacteria such as *Flavobacterium* show antagonistic relationships with these microalgae. Positive and negative feedback mechanisms maintain community balance and system robustness. The three components complement each other: microalgae primarily drive photocatalytic nitrogen and phosphorus removal, cyanobacteria provide nitrogen fixation and stability, and functional bacteria participate in organic matter degradation and nitrogen cycling, achieving synergistic metabolism through material exchange. Microbial ecological analysis confirms that a stable community structure and internal interaction mechanisms are the core of efficient and stable ammonia nitrogen reduction. This technology offers precise regulation, stable operation, and is environmentally friendly and economical, and can be widely applied to the treatment of various types of ammonia nitrogen-polluted water bodies.

The invention discloses a method for preparing an organic fertilizer by treating agricultural wastes and the organic fertilizer. According to the method, agricultural wastes which are high in carbon-nitrogen ratio and difficult to ferment and decompose are treated, and a biological organic matter fertilizer capable of quickly repairing the microbial ecology and organic state of soil is prepared by a fermenting and decomposing process. The method comprises the following steps: preparing a compost base material which is agricultural waste; a fermentation decomposition agent is added into the compost base material, the weight of the added fermentation decomposition agent is 0.3%-0.5% of the weight of the compost base material, and the effective viable count of the fermentation decomposition agent is not smaller than 1.5 billion per gram; and cultivating the compost base material added with the fermentation decomposition agent at a first preset temperature for a first preset time. According to the invention, agricultural wastes, especially high-carbon-nitrogen-ratio agricultural wastes which are most difficult to treat, can be completely fermented and decomposed into organic fertilizers, so that the problem that the agricultural wastes are difficult to treat is solved, and the agricultural wastes can be used as high-quality fertilizers.

The present invention relates to the technical field of membrane components for water purification, and in particular to an EHBR membrane component integrated with aeration and oxygenation and a preparation method thereof, comprising: a membrane shell and a membrane unit, wherein the membrane shell is fixedly mounted at both ends of the membrane unit; the EHBR membrane is an EHBR membrane containing nanocarbonates, and the EHBR membrane is provided with a microporous region caused by erosion; the membrane unit is provided with adjacent aeration regions and oxygenation regions, and the aeration region is formed by the microporous region. The preparation method comprises immersing part of the membrane units of the uncorroded EHBR membrane component into a heated acid solution to form micropores. The present invention solves the problem that the EHBR membrane component in the prior art has a single function and cannot perform water aeration and oxygenation. The provided EHBR membrane component integrated with aeration and oxygenation has the advantages of relatively independent aeration and oxygenation, integrated water aeration and oxygenation, and no interference with the microbial ecology around the EHBR membrane.

The invention discloses a method for establishing microbial ecology in red mud. The method comprises the following steps: A, adding an organic neutralizer into the red mud, uniformly mixing, and enabling the pH value of the red mud to be 8.5-9.5; b, adding a carbon source substance, a nitrogen source substance, a structure improving substance and naturally collected soil into the red mud in the step A, uniformly mixing, standing and fermenting at 15-40 DEG C for not less than 7 days, and stirring once every 28 hours during the period; the pH value of the fermented red mud is 8.3-9.3, the content of microorganisms is not less than 1.005 * 10 < 5 > / g, and the content of organic matters is not less than 3%. By optimizing the raw material ratio and the fermentation process, a microenvironment suitable for growth of microorganisms is created, rich microbial communities are introduced and cultured, soil improvement of the red mud is achieved, and an efficient solution is provided for ecological restoration of the red mud storage yard.

This invention relates to the field of wastewater treatment technology, and in particular to a coupled control system and method for deep denitrification of wastewater, comprising a real-time water quality monitoring module, a microbial twin modeling module, a real-time prediction module, an intelligent decision-making module, an actuator module, and a predictive control module. Compared to existing technologies that typically employ passive feedback control strategies based on effluent water quality indicators, which suffer from strong lag and are unable to cope with drastic fluctuations in influent load, this invention elevates the control objective from traditional process parameter setpoints to direct optimization of microbial ecological functions by constructing a digital twin capable of reflecting the functional state of the microbial community in real time. This represents a fundamental leap from "controlling process parameters" to "regulating microbial ecology," enabling intervention at the root of the reaction process and giving the system proactive health management capabilities, thereby significantly improving the stability of treatment efficiency and the level of intelligence in handling complex operating conditions.

The invention provides a method for inducing a lyogenic phage based on xanthohumol and application thereof, which are suitable for inducing the lyogenic phage in an escherichia coli host and show a remarkable induction effect. Compared with induction by using 1.5 mu g / ml mitomycin C, the fulvic acid with the final concentration of 1 mu g / ml can induce higher bacteriophage titer; in an in-vivo experiment, a mouse infected with escherichia coli is treated by orally taking xanthohumol, the bacterial load can be remarkably reduced, the phage titer is improved, no obvious toxicity exists, a CCK-8 experiment shows that the survival rate of HepG2 cells given with xanthohumol is remarkably higher than that of HepG2 cells given with mitomycin C group with the same concentration, and xanthohumol shows lower cytotoxicity. The method for inducing the lysogenic bacteriophage based on the xanthohumol is suitable for the fields of bacteriophage therapy, microbiology, microbial ecology and the like, and has a wide application prospect.

The invention discloses a sampling device for a soil microbial ecology test. The sampling device comprises a movable base, two vertical plates are fixedly arranged on the upper end face of the movable base, a U-shaped frame is rotatably arranged between the vertical plates, a bearing plate is slidably arranged in the U-shaped frame, a sampling barrel is fixedly arranged at the bottom of the bearing plate, and an electric push rod is fixedly arranged at the upper end of the U-shaped frame; a through hole is formed in the center of the upper end of the sampling barrel, a material pushing rod is inserted and connected into the through hole, a material pushing plate is fixedly arranged at one end, extending into the sampling barrel, of the material pushing rod, and a spring is arranged on the material pushing rod and located between the material pushing plate and the top surface in the sampling barrel in a sleeving manner; compared with the prior art, the soil sampling device has the advantages that the bearing plate is used for driving the sampling barrel to vertically press downwards for sampling, in cooperation with the unique design of the pushing rod, the pushing plate and the spring, disturbance to a soil sample is greatly reduced in the sampling and material taking process, the original layered structure of soil is effectively reserved, and the sampling effect is improved. And a real and reliable sample basis is provided for subsequent biological analysis.

The invention relates to the technical field of microbial ecology, and discloses a high-resolution quantitative method for influence of antibiotics on microbial activity of sediment, which comprises the following steps: S1, sediment preparation and time sequence SIP culture experiment, S2, DNA extraction and DNA-SIP process, S3, quantitative polymerase chain reaction, S4, DNA sequencing of DNA-SIP density gradient, S5, sequence processing, S5, sequencing, S6, sequencing, S7, sequencing, S7, sequencing, S8, sequencing, S8, sequencing, S8, sequencing and S8, sequencing. And S6, calculating the percentage of the total community DNA of the prokaryotes marked by 13C. According to the method, time sequence culture of coast sediments is combined with DNA-SIP and high-throughput sequencing analysis, the acute influence of high-concentration sulfadimidine (SMT) in wastewater on metabolic activity of different groups in sediment prokaryotic biocenosis is successfully evaluated, and quantitative SIP (qSIP) is used for quantifying the acute influence on the high-resolution level of phylogenetic development. Results show that high-concentration SMT in the aquaculture wastewater generates stimulation or inhibition of different degrees on metabolic activity of different bacteria.

This invention discloses a temperature control system for aquaculture fermentation beds, belonging to the field of aquaculture fermentation bed temperature control technology. It includes a fermentation bed environment sensing module, a data processing intelligent decision-making module, a precise ventilation control module, an intelligent temperature control module, a humidity collaborative control module, a material turning module, and an anomaly early warning and emergency handling module. This invention constructs an intelligent decision-making system from passive response to active prediction by integrating a multi-parameter fusion algorithm, adaptive PID control, and an LSTM prediction model. Dynamic comprehensive index calculation makes the control strategy closer to the needs of microbial ecology. The improved PID algorithm achieves parameter self-tuning through gradient descent, significantly improving control accuracy and stability. The LSTM model predicts future environmental changes based on historical data, combined with a dynamic threshold configuration unit, enabling the system to automatically optimize control parameters according to the aquaculture stage and raw material ratio, achieving intelligent adaptation across scenarios.

The invention discloses a device for reducing nitrous oxide emission by measuring root system-arbuscular mycorrhizal fungi interaction, and relates to the technical field of soil ecology and microbial ecology tests, the device comprises three experiment assemblies which are arranged in sequence, each experiment assembly comprises an experiment groove, a partition plate is arranged in the middle of each experiment groove, and the partition plate is arranged in the middle of each experiment groove; the partition plate is used for dividing the experiment groove into a planting area and a hypha area. The collecting frame is partially embedded into the hypha area, and a water tank is arranged on the periphery of the collecting frame; the top of the gas collection top seat is closed, the bottom of the gas collection top seat is open, and the opening edge of the gas collection top seat is immersed into the water tank; the exhaust pipe assembly is arranged on the gas collection top seat and is used for conveying the gas in the gas collection top seat to the collection barrel assembly; the spray pipes are distributed on one side of the partition plate at intervals, the spray pipes are provided with spray head assemblies spraying towards the partition plate, and the influence of arbuscular mycorrhizal fungi on soil nitrous oxide emission is revealed through contrast experiments.

The application discloses a device for measuring root-arbuscular mycorrhizal fungus interaction for reducing nitrous oxide emission, and relates to the technical field of soil ecology and microbial ecology test, and comprises three experimental components arranged in sequence, wherein the experimental components comprise: an experimental tank, a partition plate is arranged in the middle of the experimental tank, and the partition plate is used for separating the experimental tank into a planting area and a mycelium area; a collecting frame is partially embedded in the mycelium area, and a water tank is arranged around the collecting frame; a gas collecting top base is closed at the top and open at the bottom, and the open edge of the gas collecting top base is immersed in the water tank; an exhaust pipe assembly is arranged on the gas collecting top base and used for conveying the gas in the gas collecting top base to a collecting cylinder assembly; and a plurality of spray pipes are distributed at intervals on one side of the partition plate, and the spray pipes are provided with a spray head assembly which sprays towards the partition plate. The device discloses the influence of arbuscular mycorrhizal fungi on soil nitrous oxide emission through a control experiment.