4 results about "Reductive dechlorination" patented technology

This invention discloses a method for remediating halogenated hydrocarbon pollution based on functional plasmid transformation, belonging to the field of environmental microbial remediation technology. The method first extracts plasmid DNA carrying functional genes from organobacteria with reductive dechlorination capabilities (organobacteria with halogen respiration). Then, the functional plasmid DNA and electron donors are directly added to halogenated hydrocarbon-contaminated samples containing indigenous microorganisms to construct a bio-enhanced remediation system. Finally, the system is cultured under anaerobic conditions to transform the functional plasmid into the indigenous microorganisms, thereby endowing or enhancing the indigenous microorganisms with the ability to reductively dechlorinate halogenated hydrocarbon pollutants. This invention avoids the ecological risks of directly introducing exogenous live bacteria. Through plasmid-mediated horizontal gene transfer, it rapidly activates and enhances the function of in-situ microbial communities, significantly improving the degradation rate and thoroughness of halogenated hydrocarbon pollutants, and providing an efficient, safe, and universally applicable technical means for in-situ bioremediation of contaminated sites.

This invention discloses a method for preparing and applying biochar-based microbial agents for reductive dechlorination, belonging to the fields of environmental engineering and pollution treatment engineering technology. This invention solves the problems of existing anaerobic reductive dechlorination reactions where dehalogenating respiratory bacteria struggle to withstand load shocks and highly heterogeneous environmental media, and are difficult to stably and scalably prepare biochar-immobilized microbial agents. This invention utilizes surfactants to modify biomass biochar, enhancing its hydrophilicity or electron transfer efficiency, and employs mixed anaerobic cultivation to ensure a more uniform distribution of dehalogenating respiratory bacteria on the modified biochar, significantly increasing the microbial loading. The resulting biochar-immobilized microbial agent material loaded with dehalogenating respiratory bacteria integrates adsorption and bioremediation functions, increasing its continuous action time in the soil while minimizing the impact of external substances on the soil. It is an ideal in-situ soil bioremediation material.

The present invention belongs to the technical field of wastewater treatment. Disclosed are a conductive material-loaded bio-electrochemical system device for removing a trichloroethylene contaminant, and a method. Wastewater to be treated containing trichloroethylene is introduced into a bio-electrochemical system device inoculated with a dechlorination mixed microbiota; microorganisms colonize the surface of a cathode carbon brush to form an electroactive dechlorinating biofilm; electrons flow through an external circuit to a cathode so as to be used by the microorganisms; nano-magnetite and activated carbon enhance the reductive dechlorination of trichloroethylene by enhancing electron transfer. The present invention can completely convert trichloroethylene to ethylene and completely reduce 500 μM of trichloroethylene within 12-15 days, couples electrochemical technology with microbial dechlorination technology, and has the advantages of low cost, environmental friendliness and in-situ repairability.

This invention discloses a method for anaerobic bioremediation of chlorinated hydrocarbons driven by PBSA as a slow-release electron donor, belonging to the field of in-situ bioremediation technology for soil and groundwater. The method is as follows: 1) A mixed dechlorination bacterial community containing obligate organohalogen-respiring bacteria and their symbiotic bacteria is cultured to the logarithmic growth phase to obtain a mixed dechlorination bacterial solution; 2) Anaerobic digested sludge is mixed evenly with the mixed dechlorination bacterial solution to obtain a mixed inoculum; 3) The mixed inoculum is added to groundwater contaminated with chlorinated hydrocarbons, and PBSA is added as a slow-release electron donor to drive the reductive dechlorination reaction of chlorinated hydrocarbons under anaerobic conditions, thereby removing the pollutants. This invention solves the problems of easy dilution and high risk of secondary pollution associated with traditional liquid carbon sources, as well as the high cost and low dechlorination efficiency of existing slow-release materials. Using PBSA as a slow-release electron donor results in an increasing dechlorination rate and high electron utilization efficiency. Industrial-grade PBSA is inexpensive, and this invention provides an efficient, green, and low-cost solution for in-situ remediation of chlorinated hydrocarbons in groundwater.