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Efficient 2, 4, 6-trichlorophenol degrading bacterial strain and separation method thereof

A technology of trichlorophenol and 3gkh2po4, applied in the direction of using microorganisms, methods based on microorganisms, biochemical equipment and methods, etc.

Inactive Publication Date: 2015-03-04
XIANGTAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, to date, there has been no report on the degradation of chlorophenolic organics by reductive dechlorination by Pseudomonas under anaerobic conditions

Method used

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  • Efficient 2, 4, 6-trichlorophenol degrading bacterial strain and separation method thereof
  • Efficient 2, 4, 6-trichlorophenol degrading bacterial strain and separation method thereof
  • Efficient 2, 4, 6-trichlorophenol degrading bacterial strain and separation method thereof

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Experimental program
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Embodiment 1

[0009] Embodiment 1: Domestication, separation and screening of chlorophenol-degrading bacteria in the present invention

[0010] Collect the activated sludge from the anaerobic treatment reactor in the wastewater internal circulation of a paper mill in Hunan, measure 1 L of the semi-fluid sludge and add it to the domestication culture bottle, then add 1 L of the domestication medium to adjust the pH to between 6.0-8.0 between. Fill the acclimatization culture bottle with nitrogen gas, and seal it with a rubber stopper with an air guide tube. One end of the air guide tube is inserted below the liquid level in the bottle, and the outer end of the bottle is inserted into the sodium hydroxide solution for liquid sealing. Put the whole set of acclimatization device in an anaerobic biochemical incubator for acclimation culture at 30-40°C. During the acclimatization process, regular samples were taken to measure the residual concentration of 2,4,6-TCP in the acclimatized sample, an...

Embodiment 2

[0011] Embodiment 2: the biological identification of bacterial strain in the present invention

[0012] 1. Morphological characteristics

[0013] (1) Morphological characteristics of colonies: white, opaque, with neat edges, round, smooth and slightly raised. (2) Morphological characteristics of bacterial cells: the bacterium is Gram-negative, short rod-shaped, 0.5-0.7 μm×1.0-2.0 μm in size, without flagella, see attached figure 2 ;

[0014] 2. Physiological and biochemical properties: able to hydrolyze glucose, sucrose and fructose, produce indole and hydrogen sulfide, liquefy gelatin and form biofilm, and test positive for catalase, lecithinase and esterase; but cannot hydrolyze Starch, V-P, methyl red, nitrate reduction, and denitrification tests were all negative. The suitable growth temperature is 30~40 ℃, and the pH is 6.0~8.0;

[0015] 3. 16S rDNA molecular identification and phylogenetic analysis

[0016] Bacterial Genomic DNA Extraction Kit was used to extract ...

Embodiment 3

[0017] Example 3: Degradation effect of strains of the present invention on 2,4,6-TCP

[0018] Inoculate the purified TB-1 strain into the acclimatization medium, and culture it statically in an anaerobic incubator at 35 °C for 2-3 days, and inoculate the culture solution to the 2,4,6-TCP concentrations were 30, 60, 90, 120, 150 mg / L dechlorinated degradation medium. Fill the culture flask with nitrogen, and culture it statically in an anaerobic incubator at 35 °C. Samples were taken every 12 hours to determine the content of 2,4,6-TCP in the sample. The results are shown in the appendix Figure 4 . And use gas chromatography-mass spectrometry to detect the intermediate products produced in the degradation process, the results are shown in the attached Figure 5 . Figure 4 The results showed that TB-1 could completely degrade 2,4,6-TCP at a concentration of 30 mg / L within 72 hours, and partially degrade it at a concentration of 150 mg / L of 2,4,6-TCP. Figure 5 The results...

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Abstract

The invention discloses a separation method of an efficient chlorophenol degrading bacterial strain, and the efficient chlorophenol degrading bacterial strain Pseudomonassp.TB-1, with the collection number of CGMCCNo.9686. obtained through the separation method. The separation method comprises the following steps: obtaining anaerobic activated sludge from an internal-circulating anaerobic papermaking wastewater treatment reactor, domesticating and screening for a long time, and separating to obtain a bacterial strain for efficiently degrading 2, 4, 6-trichlorophenol through reductive dechlorination. The bacterial strain can completely degrade the 2, 4, 6-trichlorophenol with a concentration of 30mg / L within 72 hours, and still has a degrading rate of 72% when the concentration of the 2, 4, 6-trichlorophenol (2, 4, 6-TCP) is as high as 150mg / L.

Description

technical field [0001] The invention relates to a biological treatment technology for highly toxic organic pollutants, in particular to a high-efficiency 2,4,6-trichlorophenol-degrading bacterium and a separation method thereof. Background technique [0002] Chlorophenols are refractory organic pollutants that are ubiquitous in wastewater. As an important class of organic chemical industrial raw materials, they are widely used in medicine, chemical industry and other fields; due to their antibacterial, bactericidal and insecticidal properties It is used as an insecticide, preservative, herbicide, etc., and is used in forestry and agriculture industries. Chlorophenols have strong biotoxicity, bioaccumulation, and biorefractory biodegradability, as well as the three effects of "carcinogenic, teratogenic, and mutagenic", which pose a serious threat to human health and the ecological environment. Chlorophenol biodegradation treatment technology is a method with low cost, low en...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/38
CPCC12N1/36C12N1/205C12R2001/38
Inventor 戴友芝杨基成陈跃辉万丽
Owner XIANGTAN UNIV
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