32 results about "Catalase" patented technology

This invention belongs to the fields of biotechnology and genetic engineering, and relates to a dual-enzyme fusion protein resistant to hydrogen peroxide inhibition, a polynucleotide, an expression vector, and its applications. The dual-enzyme fusion protein is composed of a glutamate oxidase mutant, a catalase mutant, and a linker peptide; the amino acid sequences of the glutamate oxidase mutant and the catalase mutant are shown in SEQ ID NO. 2 and 4, respectively, in the sequence listing. This invention improves the performance of single enzymes through enzyme molecule modification, achieves spatial synergy through fusion protein design, and enables industrial application through a continuous reaction process; it fundamentally solves the problem of hydrogen peroxide's inhibition of glutamate oxidase products; and the fusion expression simultaneously improves the stability and reusability of the dual enzymes.

The application discloses a method for promoting growth of cymbidium on a steel slag flat bed based on microbial organic matrix, and belongs to the technical field of steel slag resource utilization. The application inoculates eight kinds of bacillus agents with different concentrations on the bacterial residue to prepare the organic matrix, and then covers the matrix on the converter slag to promote the growth of cymbidium on the converter slag, and selects the bacillus strain with the best growth promotion effect and the most suitable inoculation concentration. The results show that after being inoculated with bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus, the sucrose activity of the soil is enhanced; the hydrogen peroxide activity of the soil in the four groups of treatments inoculated with the bacillus agent is stronger than that in the control group; the application of the bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus enhances the urease activity in the soil; the inoculation of the bacillus megaterium promotes the release of Ca and P elements in the soil and the absorption of Ca, P, Mg, Mn and Si elements by the plant.

The application belongs to the field of plant genetic engineering, and discloses a key gene for positively regulating salt stress and oxidation stress tolerance of rice OsHPCA1 . The gene (accession number: Os05g40770) encodes a receptor protein specifically sensing hydrogen peroxide, which is classified into the leucine repeat receptor-like kinase (LRR-RLKs) family, which is the largest subfamily in the receptor-like kinase (RLKs) family. OsHPCA1 In response to salt and oxidation stress signals, the rice salt and oxidation stress tolerance is positively regulated. OsHPCA1 The overexpression rice strain shows strong salt tolerance; and OsHPCA1 the knockout strain oshpca1 is more sensitive to salt stress. OsHPCA1 The leucine repeat receptor protein kinase OsHPCA1 encoded by the gene can positively regulate the activity of catalase C (CatC) in the rice body, enhance the H2O2 clearance efficiency under salt stress, and thus improve the salt tolerance of the rice. When OsHPCA1 overexpressed, the activity of CatC in the overexpression strain OsHPCA1 is significantly increased, the H2O2 clearance capacity is enhanced, and the salt tolerance and oxidation stress tolerance of the rice are improved.

This invention provides a cell microenvironment stabilizer that captures hydrogen ions, its preparation method, and its application, belonging to the field of biomedical technology. The preparation method of this cell microenvironment stabilizer includes the following steps: (1) mineralizing CaCl2 with TGF-β1, catalase, and polyethylene glycol-polyglutamic acid block copolymer to obtain mineralized nanoparticles; (2) preparing hydrogel microspheres under freezing conditions using a microfluidic device, and then photocrosslinking the frozen hydrogel microspheres; (3) mixing the hydrogel microspheres with the mineralized nanoparticles to prepare the cell microenvironment stabilizer. This invention prepares a mineralized hydrogel microsphere, which, as a cell microenvironment stabilizer, can be used to block the NLRP3 cascade in chronic inflammation, capturing excess hydrogen ions through the mineralization layer to neutralize the acidic microenvironment. This micro / nano bioreactor based on hydrogel microspheres can effectively interfere with NLRP3 in the microenvironment of degenerated tissues from inside and outside the cells, inhibiting chronic inflammation.

This invention relates to the field of catalytic degradation technology for organic pollutants, and particularly to an iron-modified biochar immobilized catalase, its preparation method, and its application. The preparation method of the iron-modified biochar immobilized catalase includes the following steps: mixing iron-modified biochar, catalase solution, and an extracellular polymeric substance; performing an immobilization and cross-linking reaction; and separating the solid and liquid phases to obtain the iron-modified biochar immobilized catalase; the extracellular polymeric substance is secreted by *Goldenella*. This invention uses an extracellular polymeric substance instead of traditional glutaraldehyde as a cross-linking agent, avoiding the hazards associated with glutaraldehyde. Simultaneously, the prepared iron-modified biochar immobilized catalase exhibits higher enzyme activity and superior pollutant degradation performance.

The application discloses a method for synthesizing catalase nanocapsules based on gas-liquid two-phase flow microfluidic technology and application. Nitrogen is used as a gas phase, and a liquid phase containing acrylated catalase, a monomer mixture and a crosslinking agent is used to form Taylor flow in a microfluidic chip. The internal vortex of the gas-liquid interface is used to enhance the mixing efficiency, and in-situ radical polymerization on the surface of the enzyme is realized. By screening six microfluidic chips with different structures, the geometric parameters of the chip and the hydrodynamic conditions are optimized, and the particle size uniformity and enzyme activity retention rate of the nanocapsules are significantly improved. The application also discloses a continuous flow reactor system for loading the obtained nanocapsules on macroporous resin microspheres to construct a continuous flow reactor system, and the system is used for efficient degradation of hydrogen peroxide in semiconductor wastewater. The method has the advantages of high mixing efficiency, mild reaction conditions, continuous production and the like, the obtained nanocapsules have the advantages of narrow particle size distribution, high enzyme activity and good stability, and are suitable for the field of industrial wastewater treatment.

The application discloses C4 corn ZmClpP6 protein and application of related biological materials thereof in regulation of high light response capability of plants. ZmClpP6 It is found that: ZmClpP6 The knockout can reduce chlorophyll content, total carotenoid content, catalase activity and copper-zinc SOD enzyme activity of corn under high light conditions, and makes corn leaves appear yellowing phenotype. It is indicated that ZmClpP6 can regulate high light response capability of plants. The application clones ZmClpP6 the gene in corn for the first time and analyzes the biological function thereof, and has important significance for cultivation of high light efficiency plants.

The present invention relates to detergent compositions comprising catalase and amylases. Furthermore, the present invention relates to methods of using the detergent compositions.

This invention provides a method for preparing and applying a nanosystem for regulating the osteogenic microenvironment. The nanosystem comprises catalase, lactate oxidase, and a strontium-containing mineralized nanolayer. Experiments demonstrate that this nanosystem can effectively treat bone aging-related diseases, and that catalase and lactate oxidase have a synergistic effect. This invention provides a novel treatment strategy for bone aging-related diseases and has broad prospects for clinical translation.

The application belongs to the technical field of medicine production and relates to responsive sustained / controlled release microspheres and a preparation method and application thereof.The responsive sustained / controlled release microspheres comprise, by weight fraction, 160-200 parts of acetalized dextran, 40-80 parts of polylactic acid-glycolic acid copolymer, 10-30 parts of a drug, 3-7 parts of glucose oxidase and 0.5-1.5 parts of catalase; the mass ratio of the acetalized dextran and the polylactic acid-glycolic acid copolymer is 3-5:1.The microspheres can release insulin responsively according to blood glucose, once administration can stabilize blood glucose for 20 hours, and can ensure safety while effectively reducing blood glucose, and will not cause a series of life-threatening symptoms such as hypoglycemia due to excessive insulin injection.The blood glucose maintenance cycle can be adjusted according to the dose of the microspheres, and the purpose of long-term stable blood glucose is achieved.At the same time, the size and performance of the microspheres are uniform and stable.

The application discloses a kind of nucleic acid nano vesicles based on sulfonium copolymerization peptide and its preparation method and application.The vesicle is formed by the electrostatic self-assembly of a kind of sulfonium copolymerization peptide and polynucleotide, and is stable nano vesicle with hollow structure.The vesicle is different from conventional solid nanoparticles, and its unique hollow structure provides space for co-loading other therapeutic agents (such as enzymes, chemotherapeutic drugs).The application also provides a preparation method of the nano vesicle, and its application as a carrier for simultaneously loading nucleic acid drugs and other active ingredients for synergistic treatment.The nano vesicle has extraordinary long-term storage stability, and can deliver siRNA and catalase to tumor sites, block CD47 "don't eat me" signal through gene silencing, and enzymatically degrade immunosuppressive factor H2O2 in tumor microenvironment, thereby remodeling tumor immune microenvironment, efficiently activating macrophages, dendritic cells and T cells, and producing strong synergistic anti-tumor immune effect.

The application discloses a drug delivery system for remodeling intestinal uric acid homeostasis and a preparation method, a pharmaceutical composition and an application thereof, and belongs to the field of biomedical technology. The drug delivery system comprises a probiotic active biological carrier with autonomous movement capability, the surface of the probiotic active biological carrier is loaded with uricase and peroxidase-like active nanoscale enzyme through electrostatic interaction, and the probiotic active biological carrier is externally coated with a pH-responsive protective shell. The uricase catalytically degrades intestinal uric acid, the nanoscale enzyme synchronously removes hydrogen peroxide byproducts to maintain catalytic stability, and the shell protects the active ingredients in gastric acid and realizes targeted release and long-term retention in the intestine by responding to dissociation. Moreover, the drug delivery system has high targeting property, high safety and can efficiently remodel intestinal uric acid homeostasis.

This invention relates to the application of a melanin-catalase nanocomposite (MAC) targeting intestinal mucosal neutrophils in the preparation of drugs for treating inflammatory bowel disease, belonging to the field of novel applications of nanomedicine. It addresses the challenges of catalase oral delivery being easily degraded and inactivated by gastrointestinal proteases and difficult to precisely target neutrophils. This invention is the first to discover a MAC prepared by in-situ polymerization of dopamine on the surface of catalase, which can be used for the treatment of inflammatory bowel disease. This nanocomposite, through oral delivery, precisely targets intestinal mucosal infiltrating neutrophils and maintains structural stability in the gastrointestinal fluid environment, effectively protecting catalase activity. It actively targets intestinal mucosal infiltrating neutrophils through the high affinity of melanin for dopamine D2 receptors, synergistically exerting a triple effect of hydrogen peroxide decomposition, hypochlorous acid scavenging, and iron ion chelation, effectively inhibiting the formation of extracellular traps in neutrophils.

This invention belongs to the field of plant genetic engineering and relates to the application and method of reducing the expression of the Zm7649 gene in improving plant stress resistance. This invention utilizes an EMS mutant with premature termination of expression. Zm7649-ems Heterologous overexpression with Arabidopsis thaliana Zm7649-OE Verified Zm7649 The function of negatively regulating salt stress and high temperature stress was studied, and its preliminary analysis was performed. Zm00001d027649 The molecular mechanism regulating stress involves the interaction between Zm7649 and a maize catalase (Zm4848), with ubiquitination degrading Zm4848 and reducing its protein stability. This invention provides new genetic resources and theoretical basis for maize stress-resistance breeding or germplasm innovation.

The application discloses a processing method for reducing heterocyclic amines in barbecue meat while maintaining characteristic flavor, and belongs to the technical field of food processing. The application solves the technical problems of high heterocyclic amine generation in the high-temperature roasting process of the existing barbecue method and insufficient flavor caused by simply reducing the temperature. The method comprises the following steps: soaking fresh meat pieces in a phosphate buffer solution containing glucose oxidase and catalase for low-temperature treatment after the fresh meat is cut into pieces; preparing a flavor precursor coating liquid composed of xylose, cysteine, glycine, peanut oil and water and performing high-speed shear emulsification treatment; immersing the drained meat pieces in the coating liquid to make the surface uniformly adhere; and then placing the coated meat pieces in a far-infrared oven for roasting while inserting a probe type thermometer to monitor the center temperature. The application is mainly used in the field of barbecue meat processing and can effectively reduce the generation of heterocyclic amines while effectively maintaining the characteristic flavor of the roasted meat.

This invention relates to the field of biomaterials technology, specifically to a method for preparing and applying a soluble bifunctional conductive microneedle patch. The preparation method includes the following steps: (1) adding low molecular weight hyaluronic acid and catalase to deionized water to obtain an HA-CAT solution; (2) adding N,N-dimethylformamide and methacrylic anhydride to the low molecular weight hyaluronic acid solution to obtain methacryloyl hyaluronic acid (HAMA); (3) dissolving a photoinitiator and methacryloyl hyaluronic acid in deionized water, adding black phosphorus nanosheets (BP) to obtain an HAMA-BP solution, filling the needle tip with the HAMA-BP solution, and filling the base with the HA-CAT solution. This conductive microneedle patch possesses excellent mechanical properties and biocompatibility. The black phosphorus nanosheets respond to changes in pH and continuously release reactive oxygen species in hypoxic environments.

The present invention relates to a method of treating tissue of a subject infected with a catalase-positive microorganism, the method comprising the steps of: applying light having a wavelength of about 400 nm to about 430 nm to the tissue of the subject infected with the catalase-positive microorganism, wherein catalase is inactivated, and contacting the tissue with a composition comprising a dilute peroxide solution. The present invention also relates to a device or light source for delivering light-activated pulsed light to treat tissue of a subject infected with a catalase-positive microorganism, wherein the light-activated pulsed light has a wavelength of about 400 nm to about 430 nm.

This invention belongs to the field of enzyme engineering technology, specifically relating to an EcCAT mutant of catalase and its applications. The mutant is obtained by mutating at least one amino acid from the following positions in SEQ ID NO:1: Ile (126th position), Ser (167th position), Val (199th position), Gly (200th position), Asn (201st position), Phe (206th position), Ala (211th position), Phe (214th position), Gly (273rd position), Ala (389th position), Phe (391st position), Leu (407th position), and Gly (410th position). Compared to wild-type catalase, the EcCAT mutant of this invention exhibits higher catalytic efficiency. When used in combination with pyridoxine 5'-phosphate oxidase, the whole-cell catalytic synthesis of pyridoxal 5'-phosphate can be completed in just 5 hours, with a conversion rate exceeding 99%.

The invention belongs to the technical field of nano materials and biological catalysis, and particularly relates to a preparation method of cerium atom doped palladium particle nano enzyme. The method comprises the following steps: uniformly mixing palladium chloride and cerium chloride which are used as metal precursors, phloroglucinol which is used as a reducing agent and a mixed solution of oleylamine and octadecene which is used as a solvent and a dispersion medium, carrying out high-temperature reaction in a closed reaction kettle, and centrifuging, washing and drying a reaction product to obtain the Ce atom-doped Pd nano-particles with waxberry-shaped morphology. According to the invention, Ce is embedded into a Pd lattice in an atomic-scale dispersion form, and most of Ce is located at the junction of the Pd (111) and Pd (100) crystal faces to form a d-f orbital hybrid structure, so that the electron transfer ability of the material is significantly enhanced. The prepared nano-enzyme has excellent catalase-like activity and oxygenase-like activity at the same time under the weak acid condition, the activity of the two enzymes can be efficiently cascaded, and the catalytic activity is remarkably improved compared with that of the nano-enzyme without Pd doping.

This invention provides an application of catalase and its encoding gene in improving the salt stress resistance of cucumber, belonging to the field of plant genetic engineering technology. In this invention, the gene CmoCAT2 from pumpkin is selected. An overexpression vector is constructed, and a pumpkin rootstock overexpressing the CmoCAT2 gene is created using Agrobacterium-mediated transformation. Using cucumber as the scion, it is verified that the mRNA of this gene can be transported long distances from the rootstock to the scion and can be translated locally within the scion, thus improving the salt stress resistance of cucumber. This method can cultivate salt-tolerant cucumber plants, adapting to the needs of cucurbit cultivation in saline-alkali land and saline-alkali soils in protected cultivation facilities.

This invention discloses a method for enhancing drought resistance and improving growth quality of Chinese cabbage based on nitrogen regulation, comprising the following steps: S1, selection of test materials and seedling preparation; S2, seedling cultivation and establishment treatment; S3, nitrogen regulation treatment; S4, drought stress response and field management; S5, harvesting and quality maintenance. Ammonium nitrogen is prepared from 99% analytical grade ammonium sulfate, and glycine is prepared from 99% analytical grade reagent. This invention uses 70% NH4+. + A nitrogen ratio of -N + 30% Gly can effectively enhance the photosynthetic characteristics and antioxidant capacity of pak choi under drought stress. This ratio can increase the transpiration rate of pak choi leaves to over 7.93 mmol·m⁻²·s⁻¹, and the net photosynthetic rate reaches 14.09 µmol·m⁻²·s⁻¹, which is significantly higher than other nitrogen ratio treatments. At the same time, it can increase the activity of root catalase (CAT) and peroxidase (POD), reduce malondialdehyde (MDA) content, alleviate drought-induced membrane lipid peroxidation damage, and enhance the plant's tolerance to drought stress.

This invention provides a method for detecting non-lactic acid bacteria in *Lactobacillus rhamnosus*. Specifically, the detection method includes: 1) adding the sample to a non-selective culture medium for enrichment to obtain an enriched sample; 2) streaking the enriched sample onto a non-lactic acid bacteria selective culture medium plate and culturing it; 3) performing a catalase test to confirm the type of microorganisms in the non-lactic acid bacteria selective culture medium plate cultured in step 2).

This invention provides iron-based nanoparticles for ultrasound sensitization and their application in sonodynamic therapy of solid tumors. The nanoparticles have a core-shell structure, with the core consisting of dihydroporphyrin e6 (Ce6) molecules and Fe. 3+ The ions are formed through coordination self-assembly, with a shell consisting of cell membrane nanovesicles derived from engineered probiotics (such as E. coli Nissle 1917) and overexpressing catalase on their surface. These nanoparticles can target and accumulate in the solid tumor microenvironment, improving hypoxia through catalytic oxygen production, inducing ferroptosis in tumor cells, and significantly sensitizing ultrasound-mediated sonodynamic therapy. They can also be combined with PD-1 monoclonal antibodies to produce synergistic immunotherapeutic effects, effectively inhibiting the growth of various solid tumors such as renal cell carcinoma and melanoma.

This application provides a tumor phototherapy formulation, its preparation method, and its application, belonging to the field of biomedical technology. The tumor phototherapy formulation includes a carrier, a pH-responsive polymer chain grafted onto the surface of the carrier, and catalase and indocyanine green loaded onto the pH-responsive polymer chain and the surface of the carrier. The carrier comprises polydopamine nanoparticles with a mesoporous cup-like structure. This formulation not only possesses targeted motility, achieving efficient accumulation at the tumor site by utilizing the hydrogen peroxide concentration gradient and pH-responsive motility behavior between the tumor microenvironment and the normal physiological environment, but also, through effective combination and synergistic effects with the suitable photosensitizer indocyanine green, combines and significantly enhances the photothermal and photodynamic therapeutic effects, achieving highly efficient tumor killing.

The application discloses a kind of nano-carriers for co-loading PD-1 antibody and catalase. Specifically, the nano-carriers contain: 30~60 wt% neutral phospholipid, 20~50 wt% anionic phospholipid, 1~20 wt% apolipoprotein and / or its mimetic peptide, 0.1~10 wt% protamine, 0.01~5 wt% hyaluronic acid, 1~10 wt% aPD-1 antibody and 1~10 wt% catalase, based on the total weight of the nano-carriers being 100 wt%. The nano-carriers of the application can synergistically enhance the therapeutic effect on breast cancer by co-encapsulating PD-1 antibody and catalase.

This invention pertains to genetic engineering and relates to an orally administered engineered microbial system and its applications. Through genetic manipulation techniques such as genome integration, a dominant mutant of the nicotine-degrading enzyme Nox is displayed on the surface of Bacillus subtilis spores. This mutant is then coupled with catalase or peroxidase, and genes related to spore germination and the system's own recombinant enzymes are knocked out to construct a strain with self-eliminating resistance genes. This invention utilizes orally administered engineered bacteria to colonize the intestines. The "molecular degradation machine and metabolic siphon" effect produced by the engineered bacteria continuously degrades nicotine diffused from the bloodstream, thereby significantly reducing the concentration of nicotine binding to central nicotine receptors. This fundamentally weakens the dopamine reward effect induced by smoking, effectively breaking the positive feedback loop of "smoking-pleasure," and providing a new method for treating nicotine dependence. As a "plug-and-play" platform technology, displaying different degradation enzymes on the surface of Bacillus subtilis spores can also degrade other harmful substances.

This invention provides a reduced single-atom nanomedicine, its preparation method, and its application, belonging to the field of nanobiomedicine technology. The single-atom nanomedicine prepared by this invention is a cerium single-atom-anchored phosphomolybdic acid nanocluster, namely Ce-POM. The preparation method includes using activated carbon as a substrate, adding a phosphomolybdic acid solution for loading, subsequently introducing cerium(III) acetylacetone hydrate for mixing and stirring, and finally calcining and reducing with hydrogen in a tubular furnace to obtain the Ce-POM single-atom nanomedicine. The Ce-POM nanomedicine of this invention possesses peroxidase-like and catalase-like activities. Through highly efficient multi-enzyme activity and charge migration, it promotes the consumption of reactive oxygen species (ROS), reduces cellular oxidative stress damage, inhibits ferroptosis, improves the microenvironment of neural tissue in cerebral hemorrhage, and promotes neural tissue repair. Simultaneously, the decrease in ROS levels further inhibits the activation of the intracellular immune signaling pathway cGAS-STING, regulates immune system function, protects neural cell repair, and achieves the ideal therapeutic effect for cerebral hemorrhage.

This invention discloses an enzyme-catalyzed stabilized teeth whitening and bleaching composite composition and its preparation method, relating to the field of teeth whitening technology. The composition comprises three components: a whitening gel, a catalyst, and a light-cured gingival barrier resin, which synergistically achieve teeth whitening and bleaching. The whitening gel contains hydrogen peroxide, carbomer, propylene glycol, a stabilizer, water, and sodium hydroxide. The catalyst contains propylene glycol, catalase, carbomer, glycerol, sodium hydroxide, sodium benzoate, purified water, and fragrance. The resin contains polyether polyurethane methacrylate, 1,6-hexanediol dimethacrylate, triethylene glycol dimethacrylate, camphorquinone, ethyl p-dimethylaminobenzoate, tert-butylhydroquinone, fumed silica, pigment, fragrance, and MIG-type silica. This invention utilizes enzyme catalysis to achieve natural-looking whitening; the whitening gel is stable during storage and does not easily dilute or release water; the resin cures gently and has a flexible texture; and the process is controllable, making it suitable for industrial production and clinical teeth whitening treatment.