66 results about "Bacillus mucilaginosus" patented technology

The invention discloses a preparation method and application of artificial soil prepared from coal gangue, 100 parts by weight of raw materials comprise: 1-10 parts by weight of a bacillus complex microbial inoculant, 5-10 parts by weight of natural soil, 5-10 parts by weight of aluminosilicate, 5-10 parts by weight of urea, 57-82 parts by weight of modified coal gangue, and 2-3 parts by weight of moss biological crust. The preparation method of the bacillus complex microbial inoculant comprises the step of adsorbing bacillus megatherium, bacillus subtilis and bacillus mucilaginosus into biomass microspheres, so as to obtain the bacillus complex microbial inoculant. The artificial soil prepared from the coal gangue is used as a matrix, and is compounded with the matrix under the action of microorganisms, so that nutrients in the coal gangue are released, the structure and water holding performance of the coal gangue are effectively improved, heavy metal pollution is reduced, the plant growth environment is improved, and the prepared artificial soil has the characteristics of high nutrients, water retention, air permeability and the like; the ecological restoration problem of the coal gangue mountain is effectively solved, and the method has large popularization conditions in the ecological restoration direction of the coal gangue mountain.

The invention relates to the field of microorganisms, and discloses a complex microbial inoculant for preventing and treating clubroot of rape. Comprising the following raw materials in parts by weight: 10 to 12 parts of acid-resistant bacillus, 12 to 15 parts of pseudomonas fluorescens PF-5, 8 to 10 parts of bacillus mucilaginosus, 5 to 7 parts of halophilic bacillus, 8 to 10 parts of bacillus amyloliquefaciens, 12 to 15 parts of trichoderma atroviride ReTv2, 6 to 8 parts of azotobacter chroococcum, 10 to 12 parts of chitosan-gamma-polyglutamic acid microspheres, 12 to 15 parts of diatomite and 8 to 12 parts of humic acid. And 2-4 parts of algal polysaccharides. The living space of plasmodiophora brassicae is preempted by the acid-resistant bacillus; the pseudomonas fluorescens PF-5 directly strikes pathogenic bacteria and stimulates the defense system of the oilseed rape; the bacillus amyloliquefaciens releases a plurality of antibacterial substances; the trichoderma atroviride ReTv2 rapidly destroys the structure of the plasmodiophora brassicae in a manner of hyperparasitism and exoenzyme secretion.

The invention discloses a magnetic carbon-loaded slow-release microbial agent which comprises an activated carbon carrier containing ferroferric oxide nanoparticles and a compound microbial bacterium composed of bacillus megatherium, bacillus mucilaginosus, white-rot fungi and pseudomonas, and the mass ratio of the compound microbial bacterium to the activated carbon carrier is (3-4): 30. The invention further discloses a preparation method and application of the magnetic carbon-loaded slow-release microbial agent. According to the magnetic carbon-loaded slow-release microbial agent disclosed by the invention, the activated carbon containing the ferroferric oxide nano-particles is used as a carrier to load the compound bacteria, so that the stability of the microbial bacteria is improved, the microbial bacteria can better resist the impact of the environment, the service life of the microbial bacteria is prolonged, and the slow-release effect of the microbial agent in the remediation of vegetable planting soil is achieved; the nutrient absorption of the vegetables is promoted.

The invention relates to a composite microbial agent suitable for saline-alkali soil remediation of sorghum production in northeast alpine regions, and a preparation method and application thereof. The composite microbial agent comprises bacillus subtilis, bacillus mucilaginosus and pseudomonas fluorescens. Bacillus mucilaginosus capable of efficiently producing polyglutamic acid and potassium and pseudomonas fluorescens capable of secreting chitinase are separated and screened from obstacle soil in northeast alpine regions, and field trials are carried out in the obstacle soil (coastal saline-alkali soil) in the northeast alpine regions. The low-temperature slow-release fertilizer has the unique characteristics of secreting polyglutamic acid, potassium and chitinase under the low-temperature condition, is low in cost, and can effectively improve the physical and chemical properties of soil, increase the seed emergence rate, stimulate the root growth and enhance the salt resistance and alkali resistance of seedlings after being applied.

The invention provides a soil conditioner for improving soil hardening and a preparation method thereof, and relates to the technical field of soil conditioning. The soil conditioner is prepared by combining nano silicon dioxide, a straw retting material, a straw composite fermentation material, biochar, a water-retaining agent and modified starch. The straw composite fermented material is prepared by the following steps: crushing straws, mixing with sweet water, stirring, drying, carrying out microwave treatment, mixing with bacillus thuringiensis, fermenting, sterilizing, subsequently adding a composite microbial agent of bacillus subtilis, trichoderma and bacillus mucilaginosus, continuously fermenting, and drying. The modified starch is prepared by semi-gelatinizing corn starch and then carrying out microwave drying and shaping. According to the present invention, the defects in the prior art are overcome, the soil hardening phenomenon can be rapidly improved with the small addition amount, the soil porosity is increased, the activity and the diversity of the soil microorganisms are ensured, and the soil fertility is improved.

The invention discloses a compound microbial agent formula for a corn seedling stage, and relates to the technical field of agricultural microorganisms. The microbial agent comprises bacillus subtilis and bacillus mucilaginosus, auxin hormone sodium naphthalene acetate, chelated zinc and a pH regulator potassium fulvate are added, and humic acid powder is used as a carrier. The dry powder preparation with the particle size being smaller than or equal to 200 meshes and the suspension rate being larger than or equal to 90% is prepared through a specific fermentation technology and a bacterial liquid mixed adsorption and spray drying technology. Field trials show that the composition can increase the emergence rate of corn from 85% to 90%-93%, increase the dry weight of the root system by 30%, and reduce the incidence rate of white seedling disease by 70% or above. Microbe, chemistry and agronomy principles are fused, and the corn production problem in the arid region is systematically solved; chemical fertilizer dependence is reduced, degraded soil is restored, and green agricultural policy guidance is met; the average cost per mu is less than 100 yuan, and the method is suitable for large-scale planting and precise irrigation systems.

The invention relates to the field of fertilizers, and discloses a microbial agent for replacing a chemical fertilizer and a preparation method thereof, and the microbial agent comprises the following components: trichoderma harzianum with a specific dosage of 0.5-1 billion CFU / g, bacillus mucilaginosus with a specific dosage of 10-20 billion CFU / g, and bacillus subtilis with a specific dosage of 21-20 billion CFU / g. The microbial agent and a carrier are prepared into a proper application concentration, the mixture is mixed with farmyard manure according to a weight ratio of 1: 1250-2500, and the mixture is applied to crops, so that more excellent growth promoting and yield increasing effects can be achieved compared with the mixture of a compound fertilizer and the farmyard manure, an unexpected technical effect is achieved, and the microbial agent is suitable for large-scale popularization and application.

The present invention belongs to the technical field of iron-containing solid waste exploitation and microorganisms and specifically provides a method for using composite microorganisms to improve the gelling performance of iron-containing solid waste by. The provided method involves treating iron-containing solid waste with iron reducing bacteria, Bacillus aceticus, and Bacillus mucilaginosus. Under the combined action of the iron reducing bacteria, Bacillus aceticus, and Bacillus mucilaginosus, the dissolution of ions such as iron, calcium, and magnesium from the iron-containing solid waste can be accelerated, and the carbonization strength of the iron-containing solid waste is improved, so that the strength of the iron-containing solid waste as a gel material is significantly improved. It can be seen from the disclosure of the embodiments that by means of the provided method for improving the gelling performance of iron-containing solid waste, the demolding strength and the carbonization strength of iron-containing solid waste such as steel slag, stainless steel slag, and nickel slag are significantly higher than those in the prior art.

The application discloses a kind of fermentation complex microbial inoculant and preparation method and application thereof, the fermentation complex microbial inoculant includes the following weight parts of component: bacillus subtilis microbial inoculant 2-5 parts, bacillus mucilaginosus microbial inoculant 5-10 parts, yeast microbial inoculant 2-10 parts, trichoderma harzianum microbial inoculant 10-20 parts, aspergillus niger microbial inoculant 10-20 parts and streptococcus thermophilus microbial inoculant 2-5 parts;The fermentation complex microbial inoculant of the application is prepared by selecting each strain and amount, is added to pigeon manure and is fermented to prepare organic fertilizer, can better decompose macromolecular substance, significantly improve the organic matter and total nitrogen content in preparation organic fertilizer, and can better improve seed germination rate;In addition, the preparation method of pigeon manure organic fertilizer of the application is simple in process, easy to operate, and convenient for production.

The invention belongs to the field of microorganisms, and particularly discloses paenibacillus mucilaginosus JZ1 and application thereof. According to the invention, paenibacillus mucilaginosus with multifunctional characteristics is screened from biological crust. The paenibacillus mucilaginosus JZ1 strain obtained by the invention has multiple functions of dissolving phosphorus, dissolving potassium, fixing nitrogen, and producing IAA, ACC deaminase, siderophore and extracellular polymeric substance (ESP), is an environment-friendly biological material, and does not cause environmental pollution. The bacterial strain is combined with the crust algae for application, so that the effects of promoting plant growth and improving soil can be stably exerted. The method has a relatively great application prospect.

The invention discloses an acid soil conditioner containing microalgae, and belongs to the technical field of agriculture and environmental restoration. The acid soil conditioner containing the microalgae is prepared from the following components in parts by weight: 100 to 130 parts of biomass charcoal, 30 to 40 parts of vermiculite, 1 to 2 parts of brachyristichum brachyristichum, 0.1 to 0.5 part of a bacillus mucilaginosus microbial agent, 0.1 to 0.5 part of a bacillus subtilis microbial agent and 0.1 to 0.5 part of a bacillus megatherium microbial agent. According to the method disclosed by the invention, the brachyristichum brachyristichum which can be planted under an acidic condition and can generate alkaline metabolites to increase the pH value of acidic soil is selected as a main functional component, and is matched with other components with functions of loosening soil, helping the brachyristichum brachyristichum to proliferate, providing organic matters, helping the soil to release nutrients and the like; and compounding to obtain the soil conditioner capable of effectively improving acid soil.

The application discloses a method for promoting growth of cymbidium on a steel slag flat bed based on microbial organic matrix, and belongs to the technical field of steel slag resource utilization. The application inoculates eight kinds of bacillus agents with different concentrations on the bacterial residue to prepare the organic matrix, and then covers the matrix on the converter slag to promote the growth of cymbidium on the converter slag, and selects the bacillus strain with the best growth promotion effect and the most suitable inoculation concentration. The results show that after being inoculated with bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus, the sucrose activity of the soil is enhanced; the hydrogen peroxide activity of the soil in the four groups of treatments inoculated with the bacillus agent is stronger than that in the control group; the application of the bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus enhances the urease activity in the soil; the inoculation of the bacillus megaterium promotes the release of Ca and P elements in the soil and the absorption of Ca, P, Mg, Mn and Si elements by the plant.

The invention discloses a self-propagating microbial soil conditioning agent and a preparation process thereof, and relates to the technical field of soil treatment. The invention relates to a self-propagating microbial soil conditioning agent. Comprising the following components in parts by mass: 13 to 21 parts of Orson's bacteria, 11 to 22 parts of bacillus licheniformis, 10 to 15 parts of bacillus mucilaginosus, 20 to 23 parts of bacillus megatherium, 15 to 25 parts of bacillus thuringiensis, 15 to 20 parts of bacillus laterosporus, 12 to 25 parts of bacillus mucilaginosus and 240 to 365 parts of plant organic matters. The colonic microorganism soil conditioning fungicide prepared from the components can remarkably improve the soil structure, improve the soil fertility and promote crop growth. Meanwhile, due to the fact that microorganisms have the self-breeding capacity, beneficial microbial communities can be continuously provided for the soil, and the ecological balance of the soil is maintained. In addition, the microbial agent also has the advantages of preventing and treating soil diseases, reducing the use amount of chemical fertilizers and the like, and has important significance for promoting agricultural sustainable development.

The application provides an island reef coral filler compaction pile and a construction method thereof. The construction method of the island reef coral filler compaction pile comprises the following steps: (1) using a weight to hammer the ground soil body to form a cylindrical hole; (2) first filling mixed sand and stone into the hole, the mixed sand and stone is composed of coral reef gravel and calcareous sand, then pouring a microbial curing liquid and a composite additive, the microbial curing liquid is composed of a composite bacterial liquid and seawater, the composite bacterial liquid is prepared from bacillus pasteurii, bacillus cereus and bacillus mucilaginosus, and the composite additive is composed of hydroxyl citronellal and eucalyptol; then using the weight to ram the filler; (3) repeating step (2) to ram and fill the pile hole to the ground to form the compaction pile. The construction method can significantly improve the quality of the compaction pile, significantly improve the single pile bearing capacity, and effectively reduce the relative usage amount of the microbial curing liquid.

The invention relates to the technical field of soil improvement, and particularly discloses a compound microbial agent and application thereof. The compound microbial agent is prepared from the following viable bacteria: bacillus subtilis, bacillus licheniformis, bacillus circulans, bacillus mucilaginosus, nitrogen-fixing bacteria (Azobacter vinelandii), phosphate solubilizing bacteria (Acinetobacter sp.), potassium solubilizing bacteria (Sptotrophomonas cidaminiphila) and the like, and the compound microbial agent is prepared from the following active bacteria: bacillus subtilis, bacillus licheniformis, bacillus circulans, bacillus mucilaginosus, nitrogen-fixing bacteria (Azobacter vinelandii, phosphorus solubilizing bacteria (Acinetobacter sp.), potassium solubilizing bacteria (Sptotrophomonas cidaminiphila) and the like. The compound microbial agent provided by the invention can effectively improve soil ecology, promote crop growth and development, and improve crop yield and disease resistance.

The invention belongs to the technical field of soil remediation, and relates to a microalgae-microorganism compound preparation for improving saline-alkali soil and application. The microalgae-microorganism compound preparation is prepared from spirulina, chlorella, nostoc, scenedesmus obliquus, bacillus mucilaginosus, bacillus mucilaginosus and pseudomonas. The microalgae-microorganism compound preparation for improving the saline-alkali soil can improve the available phosphorus content of the soil, increase the available potassium content of the soil and effectively improve the soil; soil bulk density is reduced, and soil hardening is improved; therefore, the fertilizer retention property of the soil is enhanced, the growth and development of crop roots are facilitated, the crop yield is remarkably improved, and the application prospect is wide.

The invention belongs to the technical field of biological bacterial fertilizers, and particularly relates to a high-salt-tolerance microbial liquid complex microbial inoculant suitable for saline-alkali desertification land and a biological bacterial fertilizer thereof. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation of three stages: salt-tolerant repair bacteria Nocardia halophila, salt-tolerant functional bacteria Bacillus subtilis and Azospirillum brasilense, nitrogen-fixing functional bacteria Azotobacter chroococcum, phosphorus-solubilizing functional bacteria Pseudomonas fluorescens, and nitrogen-solubilizing functional bacteria Pseudomonas fluorescens. The bacillus mucilaginosus is used as a potassium-dissolving functional bacterium, the trichoderma asperellum is used as a biocontrol growth-promoting bacterium, and the trichoderma harzianum is used as a rhizosphere optimizing bacterium. The biological bacterial fertilizer is prepared from the liquid complex microbial inoculant. Through scientific compatibility of different strains provided by the invention, efficient improvement and crop yield increase of the saline-alkali desertification land are realized, and the strain has important economic and social values.

According to the agricultural liquid fertilizer for tomato planting, bacillus licheniformis, bacillus amyloliquefaciens, bacillus mucilaginosus and bacillus pumilus are subjected to liquid fermentation and then mixed to prepare the novel agricultural liquid fertilizer for tomato planting. According to the present invention, the tomato growth promotion effect is significant, the prevention and control effects on tomato early blight, tomato black spot pathogen, tomato fusarium wilt, tomato gray mold and tomato anthracnose are significant, the tomato survival rate can be improved, the promotion effect is provided, the fruit quality is improved, the important significance is provided for tomato planting, and the economic benefit is significant. The liquid fertilizer can change the soil flora structure, increases the soil fertility and permeability, and plays an important role in soil improvement.

The invention relates to the technical field of seed coating, in particular to a coating agent and a preparation method thereof. The coating agent comprises compound microorganisms, an antibacterial enhancer, a film-forming agent and a dispersing agent; the compound microorganisms comprise azospirillum, pseudomonas fluorescens, bacillus mucilaginosus and trichoderma harzianum; the antibacterial reinforcing agent is prepared from chitosan and epsilon-polylysine; the mass ratio of the compound microorganisms to the antibacterial enhancer is (1-3): (0.5-1). According to the coating agent and the preparation method thereof provided by the invention, the double effects of seed storage and preservation and growth promotion can be taken into account, and meanwhile, the environmental adaptability can be further improved.

The invention discloses a fermentation complex microbial inoculant as well as a preparation method and application thereof. The fermentation complex microbial inoculant comprises the following components in parts by weight: 2-5 parts of a bacillus subtilis inoculant, 5-10 parts of a bacillus mucilaginosus inoculant, 2-10 parts of a saccharomycetes inoculant, 10-20 parts of a trichoderma harzianum inoculant, 10-20 parts of an aspergillus niger inoculant and 2-5 parts of a streptococcus thermophilus inoculant. According to the fermentation complex microbial inoculant disclosed by the invention, various strains and dosages are selected, and the fermentation complex microbial inoculant is added into the pigeon droppings for fermentation to prepare the organic fertilizer, so that macromolecular substances can be better decomposed, the content of organic matters and total nitrogen in the prepared organic fertilizer is remarkably increased, and the germination rate of seeds can be better increased; in addition, the preparation method of the pigeon droppings organic fertilizer is simple in process, easy to operate and convenient to produce.

The present application relates to the technical field of bio-organic fertilizer preparation, and particularly relates to a bio-organic fertilizer prepared by microbial fermentation and a preparation method thereof, which is prepared by fermentation of raw materials and compound microbial strains, wherein the raw materials include livestock and poultry manure, crop straw, industrial organic waste residue and a humification agent, and the compound microbial strains are a ternary combination of bacillus subtilis, bacillus mycoides and saccharomyces cerevisiae, or a quaternary combination of bacillus subtilis, bacillus mycoides, saccharomyces cerevisiae and bacillus mucilaginosus. The present application adopts a multi-element compound strain combination and optimizes the ratio, and the synergistic effect of each strain is significant, which greatly improves the organic matter degradation efficiency and effective nutrient activation capacity, and compared with single strain, the microbial activity is stronger, and the nutrient supply capacity of the fertilizer is better.

The invention provides a combined biological agent as well as a preparation method and application thereof, and relates to the technical field of soil remediation. The combined biological agent is prepared from a penicillium oxalicum fermentation solution, a chaetophyllum fermentum fermentation solution, a thiamine-solubilizing clostridium fermentum fermentation solution, a nitrogen-fixing functional bacterium fermentation solution, a phosphorus-solubilizing bacillus megaterium fermentation solution, a bacillus mucilaginosus fermentation solution and a pseudomonas aeruginosa fermentation solution. According to the combined biological agent, through the synergistic effect of multiple functional strains, the effective state concentration of heavy metal in soil is effectively reduced, meanwhile, the soil fertility is improved, the survival rate and colonization ability of the agent in an extreme environment are enhanced, the vegetation coverage rate is remarkably increased, and the soil quality is improved. And an efficient solution is provided for ecological restoration of the solid waste storage yard of the nonferrous metal mining area in the northwest arid region.

The invention discloses a soil conditioner for synergistically passivating heavy metals based on pH-responsive humic acid-functional bacteria and a preparation method of the soil conditioner, and relates to the technical field of soil remediation. The preparation method comprises the following steps: mixing bacillus megatherium, bacillus mucilaginosus and azotobacter chroococcum to prepare a compound microbial agent, and activating humic acid by using acid or alkali as an activating agent to obtain pretreated humic acid; and preparing the soil conditioner by taking the compound microbial agent, the pretreated humic acid, the porous mineral matrix and the nutrient as raw materials. According to the prepared soil conditioner, passivation of various heavy metals in soil within a larger pH range is achieved through cooperation of three aspects of chemical passivation, biological mineralization and physical adsorption, and the micro-ecology of the soil can be improved.

The biological organic bacterial fertilizer is prepared from the following raw materials in parts by weight: 300 to 400 parts of fermented livestock and poultry manure, 300 to 400 parts of humic acid, 50 to 100 parts of zinc sulfate, 50 to 100 parts of boric acid, 5 to 10 parts of tetrasodium iminodisuccinate, 6 to 10 parts of compound microbial strains, 3 to 5 parts of nano activated carbon, 3 to 5 parts of polyaspartic acid and 3 to 5 parts of polyglutamic acid. The compound microbial strains comprise bacillus subtilis, bacillus licheniformis, bacillus mucilaginosus and bacillus megatherium.

The present application relates to a kind of microbial inoculant, specifically to a kind of microbial inoculant for improving subtropical secondary forest soil quality, belong to the field of biotechnology.The microbial inoculant includes A component, B component and C component;The A component includes Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus mucilaginosus;The B component includes Shewanella putrefaciens, Podospora sp. And Burkholderia sp.;The C component includes superphosphate, adhesive, soil bulking agent and water.The microbial inoculant is generated by the microbial growth-metabolism-death process of microbial residue carbon and nutrient, green, efficiently improve subtropical secondary forest soil organic matter content, phosphorus-solubilizing nitrogen-fixing, improve soil microbial community structure, promote secondary forest positive succession.

The invention discloses bacillus mucilaginosus JZ-235 as well as a preparation method, a microbial agent and application thereof, the preservation number of the bacillus mucilaginosus JZ-235 is CGMCC No.32962, the bacillus mucilaginosus JZ-235 has the advantages of high yield of urease and indoleacetic acid, efficient utilization of ammonium nitrogen and no denitrification effect, crop growth can be remarkably promoted, the yield is increased, CO3 < 2-> is generated while nitrogen (NH4 < + >) is provided for crops, and the bacillus mucilaginosus JZ-235 has the advantages of high yield of urease and indoleacetic acid and no denitrification effect. According to the present invention, with the application of the nitrogen fertilizer, the nitrogen fertilizer efficiency can be easily improved, the influence of the ammonia emission on the environment can be reduced, the nitrogen fertilizer efficiency can be easily improved through the growth and the propagation at the crop rhizosphere, the ratio of the non-denitrification functional bacteria in the soil can be increased, the soil denitrification effect can be reduced, and the nitrogen fertilizer efficiency can be improved; therefore, emission of nitrous oxide is reduced, and environmental protection is facilitated.

The invention provides a soil composition, soil and a preparation method thereof. The soil composition comprises the following components in parts by weight: 60-70 parts of coal gangue, 20-30 parts of fly ash, 2-3 parts of organic solid waste, 2-3 parts of desert sand, 2-4 parts of sludge and 1-3 parts of a biological agent, the biological agent is bacillus megatherium and / or bacillus mucilaginosus. The bacillus megaterium and / or the bacillus mucilaginosus can accelerate the decomposition of organic matters, promote the conversion of inorganic nutrients, secrete plant growth stimulant and enzyme substances, enhance the resistance of plants to diseases and promote the growth of crops. Especially, the composition can also remove pathogenic bacteria, improve the soil microbial environment and accelerate the soil ripening process. The soil formed by adopting the soil composition is relatively low in cost, the formed soil is more required by the environment, and the existing substances which are difficult to treat can be treated, so that the soil composition is more practical.

The invention relates to the technical field of cotton planting, in particular to a liquid fertilizer for cotton planting in high-salt soil and a preparation method of the liquid fertilizer, and the liquid fertilizer is prepared from the following components in percentage by volume: 35-40% of nitrohumic acid solution, 25-30% of nitrohumic acid potassium solution, 15-20% of compound microbial agent, 10-15% of plant enzyme and 3-5% of synergist. Wherein the compound microbial agent comprises one or more of bacillus subtilis, bacillus mucilaginosus and saccharomyces cerevisiae. According to the method, a nitrohumic acid-bio-organic fertilizer-complex microbial inoculant compound fertilizer system is created for the first time, the salt content of the high-salinity land is reduced in a stepped mode within 1-2 years, the planting environment of the high-salinity land is restored, and cotton planting is facilitated.

The invention relates to the technical field of soil improvement. The invention provides a saline-alkali soil improving microbial agent and a preparation method thereof. The saline-alkali soil improving microbial agent is prepared from the following raw materials in parts by weight: 15 to 20 parts of bacillus megatherium, 10 to 15 parts of bacillus subtilis, 8 to 12 parts of bacillus mucilaginosus, 5 to 8 parts of pseudomonas fluorescens, 6 to 10 parts of trichoderma harzianum, 5 to 8 parts of arbuscular mycorrhizal fungi and 3 to 5 parts of rhizobium. Through cooperative use of the strains, the salt content of the coastal salinized soil can be effectively reduced, the pH value can be adjusted, the nutrient index of the salinized soil can be improved, the content of microorganisms in the soil can be improved, and the root development of crops can be promoted. Meanwhile, the sectional fermentation process can solve the problem of strain antagonism, and the salt tolerance and viable count of the strain are improved in cooperation with a special composite culture medium, so that the saline-alkali soil improvement effect is further enhanced.

A mixed bacterial agent for microbial oil washing and its application, the present invention relates to the field of microbial technology, in particular to the technical field of a microbial oil washing agent and its application. The mixed bacterial agent for microbial oil washing of the present invention is composed of Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) ZZ‑11 and Bacillus jelly-like ( Bacillus mucilaginosus The mixed bacterial agent for microbial oil washing of the present invention has excellent emulsification performance, and its fermentation liquid has low surface tension, which can greatly reduce the interfacial tension level between the oil and the crude oil, and the oil washing efficiency can reach more than 80%.