53 results about "Bacillus mucilaginosus" patented technology

The invention relates to a composite microbial agent suitable for saline-alkali soil remediation of sorghum production in northeast alpine regions, and a preparation method and application thereof. The composite microbial agent comprises bacillus subtilis, bacillus mucilaginosus and pseudomonas fluorescens. Bacillus mucilaginosus capable of efficiently producing polyglutamic acid and potassium and pseudomonas fluorescens capable of secreting chitinase are separated and screened from obstacle soil in northeast alpine regions, and field trials are carried out in the obstacle soil (coastal saline-alkali soil) in the northeast alpine regions. The low-temperature slow-release fertilizer has the unique characteristics of secreting polyglutamic acid, potassium and chitinase under the low-temperature condition, is low in cost, and can effectively improve the physical and chemical properties of soil, increase the seed emergence rate, stimulate the root growth and enhance the salt resistance and alkali resistance of seedlings after being applied.

The invention provides a soil conditioner for improving soil hardening and a preparation method thereof, and relates to the technical field of soil conditioning. The soil conditioner is prepared by combining nano silicon dioxide, a straw retting material, a straw composite fermentation material, biochar, a water-retaining agent and modified starch. The straw composite fermented material is prepared by the following steps: crushing straws, mixing with sweet water, stirring, drying, carrying out microwave treatment, mixing with bacillus thuringiensis, fermenting, sterilizing, subsequently adding a composite microbial agent of bacillus subtilis, trichoderma and bacillus mucilaginosus, continuously fermenting, and drying. The modified starch is prepared by semi-gelatinizing corn starch and then carrying out microwave drying and shaping. According to the present invention, the defects in the prior art are overcome, the soil hardening phenomenon can be rapidly improved with the small addition amount, the soil porosity is increased, the activity and the diversity of the soil microorganisms are ensured, and the soil fertility is improved.

The application discloses a kind of fermentation complex microbial inoculant and preparation method and application thereof, the fermentation complex microbial inoculant includes the following weight parts of component: bacillus subtilis microbial inoculant 2-5 parts, bacillus mucilaginosus microbial inoculant 5-10 parts, yeast microbial inoculant 2-10 parts, trichoderma harzianum microbial inoculant 10-20 parts, aspergillus niger microbial inoculant 10-20 parts and streptococcus thermophilus microbial inoculant 2-5 parts;The fermentation complex microbial inoculant of the application is prepared by selecting each strain and amount, is added to pigeon manure and is fermented to prepare organic fertilizer, can better decompose macromolecular substance, significantly improve the organic matter and total nitrogen content in preparation organic fertilizer, and can better improve seed germination rate;In addition, the preparation method of pigeon manure organic fertilizer of the application is simple in process, easy to operate, and convenient for production.

The invention belongs to the field of microorganisms, and particularly discloses paenibacillus mucilaginosus JZ1 and application thereof. According to the invention, paenibacillus mucilaginosus with multifunctional characteristics is screened from biological crust. The paenibacillus mucilaginosus JZ1 strain obtained by the invention has multiple functions of dissolving phosphorus, dissolving potassium, fixing nitrogen, and producing IAA, ACC deaminase, siderophore and extracellular polymeric substance (ESP), is an environment-friendly biological material, and does not cause environmental pollution. The bacterial strain is combined with the crust algae for application, so that the effects of promoting plant growth and improving soil can be stably exerted. The method has a relatively great application prospect.

The invention discloses an acid soil conditioner containing microalgae, and belongs to the technical field of agriculture and environmental restoration. The acid soil conditioner containing the microalgae is prepared from the following components in parts by weight: 100 to 130 parts of biomass charcoal, 30 to 40 parts of vermiculite, 1 to 2 parts of brachyristichum brachyristichum, 0.1 to 0.5 part of a bacillus mucilaginosus microbial agent, 0.1 to 0.5 part of a bacillus subtilis microbial agent and 0.1 to 0.5 part of a bacillus megatherium microbial agent. According to the method disclosed by the invention, the brachyristichum brachyristichum which can be planted under an acidic condition and can generate alkaline metabolites to increase the pH value of acidic soil is selected as a main functional component, and is matched with other components with functions of loosening soil, helping the brachyristichum brachyristichum to proliferate, providing organic matters, helping the soil to release nutrients and the like; and compounding to obtain the soil conditioner capable of effectively improving acid soil.

The application discloses a method for promoting growth of cymbidium on a steel slag flat bed based on microbial organic matrix, and belongs to the technical field of steel slag resource utilization. The application inoculates eight kinds of bacillus agents with different concentrations on the bacterial residue to prepare the organic matrix, and then covers the matrix on the converter slag to promote the growth of cymbidium on the converter slag, and selects the bacillus strain with the best growth promotion effect and the most suitable inoculation concentration. The results show that after being inoculated with bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus, the sucrose activity of the soil is enhanced; the hydrogen peroxide activity of the soil in the four groups of treatments inoculated with the bacillus agent is stronger than that in the control group; the application of the bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus enhances the urease activity in the soil; the inoculation of the bacillus megaterium promotes the release of Ca and P elements in the soil and the absorption of Ca, P, Mg, Mn and Si elements by the plant.

The invention discloses a self-propagating microbial soil conditioning agent and a preparation process thereof, and relates to the technical field of soil treatment. The invention relates to a self-propagating microbial soil conditioning agent. Comprising the following components in parts by mass: 13 to 21 parts of Orson's bacteria, 11 to 22 parts of bacillus licheniformis, 10 to 15 parts of bacillus mucilaginosus, 20 to 23 parts of bacillus megatherium, 15 to 25 parts of bacillus thuringiensis, 15 to 20 parts of bacillus laterosporus, 12 to 25 parts of bacillus mucilaginosus and 240 to 365 parts of plant organic matters. The colonic microorganism soil conditioning fungicide prepared from the components can remarkably improve the soil structure, improve the soil fertility and promote crop growth. Meanwhile, due to the fact that microorganisms have the self-breeding capacity, beneficial microbial communities can be continuously provided for the soil, and the ecological balance of the soil is maintained. In addition, the microbial agent also has the advantages of preventing and treating soil diseases, reducing the use amount of chemical fertilizers and the like, and has important significance for promoting agricultural sustainable development.

The application provides an island reef coral filler compaction pile and a construction method thereof. The construction method of the island reef coral filler compaction pile comprises the following steps: (1) using a weight to hammer the ground soil body to form a cylindrical hole; (2) first filling mixed sand and stone into the hole, the mixed sand and stone is composed of coral reef gravel and calcareous sand, then pouring a microbial curing liquid and a composite additive, the microbial curing liquid is composed of a composite bacterial liquid and seawater, the composite bacterial liquid is prepared from bacillus pasteurii, bacillus cereus and bacillus mucilaginosus, and the composite additive is composed of hydroxyl citronellal and eucalyptol; then using the weight to ram the filler; (3) repeating step (2) to ram and fill the pile hole to the ground to form the compaction pile. The construction method can significantly improve the quality of the compaction pile, significantly improve the single pile bearing capacity, and effectively reduce the relative usage amount of the microbial curing liquid.

The invention relates to the technical field of soil improvement, and particularly discloses a compound microbial agent and application thereof. The compound microbial agent is prepared from the following viable bacteria: bacillus subtilis, bacillus licheniformis, bacillus circulans, bacillus mucilaginosus, nitrogen-fixing bacteria (Azobacter vinelandii), phosphate solubilizing bacteria (Acinetobacter sp.), potassium solubilizing bacteria (Sptotrophomonas cidaminiphila) and the like, and the compound microbial agent is prepared from the following active bacteria: bacillus subtilis, bacillus licheniformis, bacillus circulans, bacillus mucilaginosus, nitrogen-fixing bacteria (Azobacter vinelandii, phosphorus solubilizing bacteria (Acinetobacter sp.), potassium solubilizing bacteria (Sptotrophomonas cidaminiphila) and the like. The compound microbial agent provided by the invention can effectively improve soil ecology, promote crop growth and development, and improve crop yield and disease resistance.

The invention belongs to the technical field of soil remediation, and relates to a microalgae-microorganism compound preparation for improving saline-alkali soil and application. The microalgae-microorganism compound preparation is prepared from spirulina, chlorella, nostoc, scenedesmus obliquus, bacillus mucilaginosus, bacillus mucilaginosus and pseudomonas. The microalgae-microorganism compound preparation for improving the saline-alkali soil can improve the available phosphorus content of the soil, increase the available potassium content of the soil and effectively improve the soil; soil bulk density is reduced, and soil hardening is improved; therefore, the fertilizer retention property of the soil is enhanced, the growth and development of crop roots are facilitated, the crop yield is remarkably improved, and the application prospect is wide.

The invention belongs to the technical field of biological bacterial fertilizers, and particularly relates to a high-salt-tolerance microbial liquid complex microbial inoculant suitable for saline-alkali desertification land and a biological bacterial fertilizer thereof. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation of three stages: salt-tolerant repair bacteria Nocardia halophila, salt-tolerant functional bacteria Bacillus subtilis and Azospirillum brasilense, nitrogen-fixing functional bacteria Azotobacter chroococcum, phosphorus-solubilizing functional bacteria Pseudomonas fluorescens, and nitrogen-solubilizing functional bacteria Pseudomonas fluorescens. The bacillus mucilaginosus is used as a potassium-dissolving functional bacterium, the trichoderma asperellum is used as a biocontrol growth-promoting bacterium, and the trichoderma harzianum is used as a rhizosphere optimizing bacterium. The biological bacterial fertilizer is prepared from the liquid complex microbial inoculant. Through scientific compatibility of different strains provided by the invention, efficient improvement and crop yield increase of the saline-alkali desertification land are realized, and the strain has important economic and social values.

According to the agricultural liquid fertilizer for tomato planting, bacillus licheniformis, bacillus amyloliquefaciens, bacillus mucilaginosus and bacillus pumilus are subjected to liquid fermentation and then mixed to prepare the novel agricultural liquid fertilizer for tomato planting. According to the present invention, the tomato growth promotion effect is significant, the prevention and control effects on tomato early blight, tomato black spot pathogen, tomato fusarium wilt, tomato gray mold and tomato anthracnose are significant, the tomato survival rate can be improved, the promotion effect is provided, the fruit quality is improved, the important significance is provided for tomato planting, and the economic benefit is significant. The liquid fertilizer can change the soil flora structure, increases the soil fertility and permeability, and plays an important role in soil improvement.

The invention relates to the technical field of seed coating, in particular to a coating agent and a preparation method thereof. The coating agent comprises compound microorganisms, an antibacterial enhancer, a film-forming agent and a dispersing agent; the compound microorganisms comprise azospirillum, pseudomonas fluorescens, bacillus mucilaginosus and trichoderma harzianum; the antibacterial reinforcing agent is prepared from chitosan and epsilon-polylysine; the mass ratio of the compound microorganisms to the antibacterial enhancer is (1-3): (0.5-1). According to the coating agent and the preparation method thereof provided by the invention, the double effects of seed storage and preservation and growth promotion can be taken into account, and meanwhile, the environmental adaptability can be further improved.

The present application relates to the technical field of bio-organic fertilizer preparation, and particularly relates to a bio-organic fertilizer prepared by microbial fermentation and a preparation method thereof, which is prepared by fermentation of raw materials and compound microbial strains, wherein the raw materials include livestock and poultry manure, crop straw, industrial organic waste residue and a humification agent, and the compound microbial strains are a ternary combination of bacillus subtilis, bacillus mycoides and saccharomyces cerevisiae, or a quaternary combination of bacillus subtilis, bacillus mycoides, saccharomyces cerevisiae and bacillus mucilaginosus. The present application adopts a multi-element compound strain combination and optimizes the ratio, and the synergistic effect of each strain is significant, which greatly improves the organic matter degradation efficiency and effective nutrient activation capacity, and compared with single strain, the microbial activity is stronger, and the nutrient supply capacity of the fertilizer is better.

The invention provides a combined biological agent as well as a preparation method and application thereof, and relates to the technical field of soil remediation. The combined biological agent is prepared from a penicillium oxalicum fermentation solution, a chaetophyllum fermentum fermentation solution, a thiamine-solubilizing clostridium fermentum fermentation solution, a nitrogen-fixing functional bacterium fermentation solution, a phosphorus-solubilizing bacillus megaterium fermentation solution, a bacillus mucilaginosus fermentation solution and a pseudomonas aeruginosa fermentation solution. According to the combined biological agent, through the synergistic effect of multiple functional strains, the effective state concentration of heavy metal in soil is effectively reduced, meanwhile, the soil fertility is improved, the survival rate and colonization ability of the agent in an extreme environment are enhanced, the vegetation coverage rate is remarkably increased, and the soil quality is improved. And an efficient solution is provided for ecological restoration of the solid waste storage yard of the nonferrous metal mining area in the northwest arid region.

The invention discloses a soil conditioner for synergistically passivating heavy metals based on pH-responsive humic acid-functional bacteria and a preparation method of the soil conditioner, and relates to the technical field of soil remediation. The preparation method comprises the following steps: mixing bacillus megatherium, bacillus mucilaginosus and azotobacter chroococcum to prepare a compound microbial agent, and activating humic acid by using acid or alkali as an activating agent to obtain pretreated humic acid; and preparing the soil conditioner by taking the compound microbial agent, the pretreated humic acid, the porous mineral matrix and the nutrient as raw materials. According to the prepared soil conditioner, passivation of various heavy metals in soil within a larger pH range is achieved through cooperation of three aspects of chemical passivation, biological mineralization and physical adsorption, and the micro-ecology of the soil can be improved.

The present application relates to a kind of microbial inoculant, specifically to a kind of microbial inoculant for improving subtropical secondary forest soil quality, belong to the field of biotechnology.The microbial inoculant includes A component, B component and C component;The A component includes Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus mucilaginosus;The B component includes Shewanella putrefaciens, Podospora sp. And Burkholderia sp.;The C component includes superphosphate, adhesive, soil bulking agent and water.The microbial inoculant is generated by the microbial growth-metabolism-death process of microbial residue carbon and nutrient, green, efficiently improve subtropical secondary forest soil organic matter content, phosphorus-solubilizing nitrogen-fixing, improve soil microbial community structure, promote secondary forest positive succession.

The invention discloses bacillus mucilaginosus JZ-235 as well as a preparation method, a microbial agent and application thereof, the preservation number of the bacillus mucilaginosus JZ-235 is CGMCC No.32962, the bacillus mucilaginosus JZ-235 has the advantages of high yield of urease and indoleacetic acid, efficient utilization of ammonium nitrogen and no denitrification effect, crop growth can be remarkably promoted, the yield is increased, CO3 < 2-> is generated while nitrogen (NH4 < + >) is provided for crops, and the bacillus mucilaginosus JZ-235 has the advantages of high yield of urease and indoleacetic acid and no denitrification effect. According to the present invention, with the application of the nitrogen fertilizer, the nitrogen fertilizer efficiency can be easily improved, the influence of the ammonia emission on the environment can be reduced, the nitrogen fertilizer efficiency can be easily improved through the growth and the propagation at the crop rhizosphere, the ratio of the non-denitrification functional bacteria in the soil can be increased, the soil denitrification effect can be reduced, and the nitrogen fertilizer efficiency can be improved; therefore, emission of nitrous oxide is reduced, and environmental protection is facilitated.

The invention provides a soil composition, soil and a preparation method thereof. The soil composition comprises the following components in parts by weight: 60-70 parts of coal gangue, 20-30 parts of fly ash, 2-3 parts of organic solid waste, 2-3 parts of desert sand, 2-4 parts of sludge and 1-3 parts of a biological agent, the biological agent is bacillus megatherium and / or bacillus mucilaginosus. The bacillus megaterium and / or the bacillus mucilaginosus can accelerate the decomposition of organic matters, promote the conversion of inorganic nutrients, secrete plant growth stimulant and enzyme substances, enhance the resistance of plants to diseases and promote the growth of crops. Especially, the composition can also remove pathogenic bacteria, improve the soil microbial environment and accelerate the soil ripening process. The soil formed by adopting the soil composition is relatively low in cost, the formed soil is more required by the environment, and the existing substances which are difficult to treat can be treated, so that the soil composition is more practical.

The invention relates to the technical field of cotton planting, in particular to a liquid fertilizer for cotton planting in high-salt soil and a preparation method of the liquid fertilizer, and the liquid fertilizer is prepared from the following components in percentage by volume: 35-40% of nitrohumic acid solution, 25-30% of nitrohumic acid potassium solution, 15-20% of compound microbial agent, 10-15% of plant enzyme and 3-5% of synergist. Wherein the compound microbial agent comprises one or more of bacillus subtilis, bacillus mucilaginosus and saccharomyces cerevisiae. According to the method, a nitrohumic acid-bio-organic fertilizer-complex microbial inoculant compound fertilizer system is created for the first time, the salt content of the high-salinity land is reduced in a stepped mode within 1-2 years, the planting environment of the high-salinity land is restored, and cotton planting is facilitated.

The invention relates to the technical field of soil improvement. The invention provides a saline-alkali soil improving microbial agent and a preparation method thereof. The saline-alkali soil improving microbial agent is prepared from the following raw materials in parts by weight: 15 to 20 parts of bacillus megatherium, 10 to 15 parts of bacillus subtilis, 8 to 12 parts of bacillus mucilaginosus, 5 to 8 parts of pseudomonas fluorescens, 6 to 10 parts of trichoderma harzianum, 5 to 8 parts of arbuscular mycorrhizal fungi and 3 to 5 parts of rhizobium. Through cooperative use of the strains, the salt content of the coastal salinized soil can be effectively reduced, the pH value can be adjusted, the nutrient index of the salinized soil can be improved, the content of microorganisms in the soil can be improved, and the root development of crops can be promoted. Meanwhile, the sectional fermentation process can solve the problem of strain antagonism, and the salt tolerance and viable count of the strain are improved in cooperation with a special composite culture medium, so that the saline-alkali soil improvement effect is further enhanced.

The invention discloses a compound preparation containing nitrogen-fixing bacteria and application of the compound preparation in sugarcane yield increase, and belongs to the technical field of microorganisms. Comprising a complex microbial inoculant and biochar loaded with the complex microbial inoculant, and the complex microbial inoculant is composed of the following strains: Azospirillum brasilense ACCC 04138, Bacillus mucilaginosus ACCC 19749 and Pseudomonas fluorescens CICC 23919. Azospirillum brasilense, bacillus mucilaginosus and pseudomonas fluorescens are compounded to prepare a complex microbial inoculant, and the complex microbial inoculant is specifically combined with biochar prepared from bagasse and mushroom residues, so that the acid soil structure can be remarkably improved, and the sugarcane yield and sugar content can be increased. The invention provides a novel complex microbial inoculant for sugarcane planting, and provides a scientific basis for improvement of sugarcane yield and quality and improvement of acid soil.

The invention discloses a selenium-rich compound microbial fertilizer regenerated by waste and a preparation method thereof. The selenium-rich compound microbial fertilizer comprises a basic raw material, a compound flora, a selenium element gradient conversion and solid-liquid two-phase fermentation system and a selenium element gradient loading process, wherein the basic raw material comprises agricultural waste and industrial by-products; the composite flora is prepared by the following steps: preparing bacillus mucilaginosus, bacillus subtilis and bacillus amyloliquefaciens into a mixed bacteria solution; the step of gradient conversion of the selenium element comprises the steps that zymophyte and biochar are added into the basic raw materials, and preliminary fermentation is conducted for 5-7 days in a fermentation tank. The invention relates to the technical field of selenium-rich bacterial fertilizers. The composite flora of bacillus mucilaginosus, bacillus subtilis and bacillus amyloliquefaciens is used; efficient degradation of the lignocellulose and in-situ synthesis of the organic selenium are realized through metabolism complementation of the three. Through compounding and fermentation processes of sodium selenite and selenium ore powder, inorganic selenium is converted into bio-available nano-selenium and seleno-amino acid.

The invention discloses a process for producing enzyme selenoprotein by liquid compound fermentation of compound strains, and relates to the technical field of biological fermentation, enterococcus faecalis, aspergillus niger and bacillus mucilaginosus are adopted to obtain a mixed strain according to a specific proportion, the mixed strain is added into a breeding culture medium, sodium selenite is added after amplification, and the enzyme selenoprotein is obtained. Sodium selenite is absorbed by mixed strains and converted into enzyme selenoprotein, repeated fermentation is adopted, the mixed strains and sodium selenite are supplemented, and along with increase of the number of times of fermentation, the metabolic capacity of the strains is gradually enhanced, the content of metabolites is increased, and the content of selenoprotein is gradually increased and tends to be stable; according to the present invention, the stopping of the fermentation process can be timely determined according to the requirement on the product enzyme selenoprotein, after the repeated fermentation, the molecular weight of the enzyme selenoprotein is from 3000 Dalton to 18000 Dalton, the fermentation frequency can be selected according to the requirement, the larger the molecular weight of the enzyme selenoprotein is, the better the absorption is, and the wide application prospect is provided.

The application provides a kind of enzyme-containing leaf fertilizer for promoting yield increase of grouting period crop and a preparation method thereof, comprising: nitrogen fertilizer, phosphate fertilizer, potassium fertilizer, mixed bacteria powder and complex enzyme preparation are mixed to obtain a compound fertilizer, the mixed bacteria powder comprises Bacillus subtilis, Bacillus licheniformis, Bacillus mucilaginosus, Bacillus megaterium and lactic acid bacteria; The complex enzyme preparation comprises pectinase, cellulase and xylanase; The compound fertilizer is dispersed in a Tween 20 solution to obtain a fertilizer solution; The fertilizer solution is added to a cyclodextrin solution, and a coated modified compound fertilizer is obtained after freeze-drying; Chitosan, sodium alginate and deionized water are mixed to obtain a mixed solution; The coated modified compound fertilizer and the modified bentonite are added to obtain a composite sol; The composite sol is added dropwise into a calcium salt solution to obtain a hydrogel microbead precursor; The hydrogel microbead precursor is taken out and soaked in a sulfate solution to obtain a composite hydrogel microbead; And the enzyme-containing leaf fertilizer is obtained after freeze-drying of the composite hydrogel microbead.

This invention discloses a mushroom residue immobilized Bacillus mucilaginosus encapsulation agent and its application in pharmaceutical wastewater treatment. Specifically, the preparation method of the immobilized microbial strain of this invention includes three steps: mushroom residue carrier pretreatment and modification, Bacillus mucilaginosus activation and cultivation, and immobilization reaction. This invention uses widely available and low-cost mushroom residue as the raw material for nano-biochar. The selected solid waste mushroom residue is pretreated with an inorganic salt culture solution before drying. The carrier performance is further optimized through material modification, and then adsorbed and immobilized with a high-concentration Bacillus mucilaginosus bacterial solution. Specific excipients and processes are selected to improve the survival rate and performance stability of the immobilized agent.

The invention belongs to the field of agricultural microbial technology and agricultural product safety, and particularly relates to application of a method for preparing a functional biological flora in a targeted biological flora and host original flora coupling mode in rice cultivation. According to the method, bacillus subtilis, bacillus mucilaginosus, brevibacillus laterosporus and host original flora are subjected to coupled symbiotic culture to form specific functional flora, and the specific functional flora is compounded with nutrient carriers such as humic acid potassium fulvate and amino acid chelated trace elements to prepare the targeted biological bacterium water-soluble fertilizer. The microbial agent effectively reduces the bioavailability of heavy metals such as cadmium, lead and arsenic in soil and migration of the heavy metals to rice; meanwhile, the functional flora secretes hydrolase, oxidoreductase and the like, degrades pesticide residues such as organophosphorus and pyrethroid and decomposes the pesticide residues into non-toxic or low-toxicity substances, the method is specially used for solving the problem that heavy metal and pesticide residues in the rice in the filling stage of the rice exceed the standard, and the quality and edible safety of the rice are guaranteed from the source.

The invention relates to the technical field of biological leavening agents, in particular to a biological leavening agent and a preparation method thereof. The biological fermentation agent is prepared from the following raw materials in parts by mass: 20 to 40 parts of soybean meal, 10 to 15 parts of egg shells, 20 to 40 parts of vegetable leaves, 10 to 15 parts of plant rhizomes, 1 to 5 parts of alfalfa extract, 0.5 to 3 parts of pectinase, 0.5 to 1.5 parts of acinetobacter luffii, 0.5 to 1.5 parts of bacillus mucilaginosus, 0.5 to 1.5 parts of bacillus cereus, 6 to 8 parts of ergothioneine, 1 to 5 parts of brown sugar, 0.5 to 1.5 parts of bacillus subtilis and 15 parts of nitrification inhibitor. According to the method, the storage stability of the product is remarkably improved, long-term effective preservation is achieved, the yield of the obtained product mushroom dregs is extremely low, the product mushroom dregs can be directly used as a functional microbial agent, the total amount of organic waste can be rapidly reduced, and the comprehensive utilization efficiency of the organic waste is greatly improved.

The invention relates to the technical field of microbial agents. The invention provides a microbial agent. The microbial agent is prepared from the following components: 14 to 18 parts of bacillus megaterium, 5 to 10 parts of bacillus coagulans, 8 to 13 parts of penicillium baylii fungi, 6 to 11 parts of penicillium oxalicum fungi, 3 to 5 parts of arbuscular mycorrhizal fungi, 2 to 6 parts of bacillus mucilaginosus, 7 to 10 parts of aspergillus niger and 4 to 6 parts of paecilomyces lilacinus. According to the microbial agent disclosed by the invention, various bacteria are matched for use, so that phosphides which cannot be utilized by crops in soil can be effectively promoted to be converted into soluble phosphides which can be utilized, the content of available phosphorus in the soil is increased, the effectiveness of phosphorus is improved, absorption and utilization of the crops are ensured, and the growth and development of the crops are promoted.

The invention discloses a preparation method of an active bacterium enzyme for strong rooting and seedling strengthening of crops, and relates to the technical field of crops. Comprising the following steps: screening to obtain a compound strain with rooting promoting activity, wherein the compound strain comprises bacillus subtilis, bacillus mucilaginosus and trichoderma harzianum; respectively carrying out expanding culture on the compound strains to obtain seed solutions of the strains; according to the application, the bacillus subtilis secretes plant growth regulators such as indoleacetic acid and the like to directly stimulate division and elongation of root system cells, meanwhile, antibacterial substances are generated to inhibit soil-borne diseases, the bacillus mucilaginosus can efficiently dissolve phosphorus and potassium, insoluble nutrients in soil are converted into available nutrients which can be absorbed by crops, nutritional support is provided for growth of seedlings, and the growth of the seedlings is promoted. Trichoderma harzianum can antagonize pathogenic bacteria, can generate chitinase and other enzymes, promotes thickening of root cell walls, and enhances the stress resistance of seedlings.