688 results about "Bacillus mucilaginosus" patented technology

The invention relates to a group of alive microbial preparations for preparing compound microbial fertilizer and the preparation method thereof. The alive microbial preparations include a microbial inoculant and an organic material degradation agent. The microbial inoculant includes Bacillus megaterium, Bacillus licheniformis, Bacillus mucilaginosus, Bacillus laterosporus, Azotobocter chroococcum, etc. The organic material degradation agent includes Bacillus megaterium, Bacillus licheniformis, Bacillus subtilis, Bacillus circulans, Saccharomyces cerevisiae, Candida lipolytica, Bacillus pumilus, Bacillus cereus, Streptomyces griseus, Pseudomonas alcaligenes, Pseudomonas cepacia, Streptococcus salivarius subsp. Thermophilus, Geobacillus stearothermophilus, Streptomyces Thermophilus, Geotrichum candidum and Aspergillus oryzae. The preparation method comprises the following steps: carrying out submerged fermentation, directly mixing the bacterial liquids and stirring to obtain a liquid preparation; or mixing with an absorbent to obtain a solid preparation. The alive microbial preparations have the advantages of improving soil fertility, promoting plant growth, enhancing anti-disease, anti-insect and anti-drought capacities of plants, improving quality of agricultural products and so on.

The invention relates to an inorganic-organic microbial compound fertilizer and a preparation method thereof. The inorganic-organic microbial compound fertilizer is characterized by comprising ordinary high-concentration three-element compound fertilizer, humic acid, fly ash, microbial compound bacteria powder and other elements, wherein the microbial compound bacterial powder is obtained by mixing bacterial powders which are prepared by respectively fermenting and culturing photosynthetic bacteria, yeast, lactic acid bacteria, bifidobacterium, bacillus subtilis, bacillus licheniformis, bacilluslaterosporus, bacillus megaterium, bacillus mucilaginosus, azotbacteria chroococum and actinomycetes. In the inorganic-organic microbial compound fertilizer, the microbial content of the yeast is no less than 2.0*108cfu/g, and the microbial contents of the other strains are all no less than 2.0*109cfu/g. The inorganic-organic microbial compound fertilizer can centralize the advantages of an organic fertilizer, an inorganic fertilizer and microorganisms, thereby enhancing the stress resistance and the disease resistance of crops, enhancing the fertilizer absorption ability of the crops, lowering the soil hardening degree and improving the soil ecological environment and the quality of the crops.

The invention relates to a compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof, and belongs to the field of biotechnology and environmental protection. Multi-thallus compound microbial powder is prepared from the compound microbial agent according to the weight percentage of living microbes to the total amount of compound microbial powder as follows: 10%-15% of bacillus subtilis, 10%-15% of aspergillus niger, 10%-15% of bacillus mucilaginosus, 10%-15% of enterococcus faecalis, 8%-12% of bacillus licheniformis, 8%-12% of bacillus megaterium, 8%-12% of pseudomonas fluorescens, 5%-8% of lactobacillus plantarum, 5%-8% of bacillus polymyxin and 6%-8% of streptococcus thermophiles. The compound microbial agent has the effects of degrading antibiotic and pesticide residues, fermenting and composting organic matter, acting as functional fertilizer and repairing the environment, and can solve the problems of secondary pollution caused by antibiotic residues in culture feces and resource utilization of organic waste and realizes biodegradation of the antibiotic and pesticide residues in soil when applied to the agricultural ecological environment, thereby being of great value and practical significance in restoration of agricultural ecological environment and protection of human health.

Disclosed are a tobacco composite microbial fertilizer and its preparation method. The tobacco composite microbial fertilizer contains an active functional bacteria ingredient, an organic fertilizer ingredient and an inorganic composite fertilizer ingredient, wherein the active functional bacteria ingredient contains phosphate solubilizing bacteria-Bacillus megaterium powder, potassium bacteria-Bacillus mucilaginosus powder, tobacco growth-promoting rhizobacteria-Bacillus amyloliquefaciens powder and tobacco endophytic antagonistic bacteria-Brevibacillus laterosporus powder; and the organic fertilizer ingredient contains decomposed manure, a rapeseed cake fertilizer and straws. The invention also relates to a preparation method of the tobacco composite microbial fertilizer. The fertilizerprovided by the invention has advantages of an organic fertilizer, an inorganic fertilizer and a microbial fertilizer. According to the invention, utilization rate of the fertilizer can be raised, soil organic matters can be increased, tobacco plant rhizosphere microbial proliferation can be promoted, soil physical and chemical property and biological activity can be improved, comprehensive and balanced nutrients can be provided for the early growth of tobacco seedling, the occurrence of soil borne disease can be decreased, and flue-cured tobacco output and quality can be substantially improved.

The invention discloses a method for repairing a crack of a cement-based material. According to the method, carbonic anhydrase microorganisms are adopted for generating carbonic anhydrase used for catching CO2, CO2 is promoted to be converted into CO3<2->, and the deposition of calcium carbonate within a surface area of the crack is accelerated; the method has the advantage that the repair speed is faster than that of other methods for repairing the crack through the microorganisms. The method comprises the following steps of: inoculating bacillus mucilaginosus to a culture medium; culturing; preparing bacillus mucilaginosus concentrated bacteria liquid; immobilizing bacteria on carriers; uniformly burying calcium sources into the cement-based material during forming the cement-based material; concentrating the carriers with the bacteria immobilized into a range of 5mm under a surface area of a test part; manufacturing the crack after a part to be tested is cured; transferring into a thermostatic waterbath; continuously charging air for maintaining and repairing. The repair test result shows that the osmotic coefficient is greatly reduced after repairing for 5 days, and the crack is repaired completely after continuously repairing for 30 days.

The invention discloses a composite microbial inoculum organic multielement compound fertilizer which is prepared from microbial inoculum, organic component and inorganic fertilizer. The microbial inoculum is a composite microbial inoculum which comprises the following three types of microbial inocula: resource type microbial inoculum, which contains Bacillus megaterium, thiobacillus thiooxidans, Bacillus mucilaginosus and Bacillus circulans; environment-friendly microbial inoculum, which contains Arthrobacter and Alcaligenes; and antibiotic microbial inoculum, which contains Streptomycs micuoflavus and Streptomyces jingyangensis. The organic component is one or more of cereal crop straw, subsidiary agricultural product dreg, sugar mill bagasse, bran coat, municipal and rural domestic waste, and livestock and poultry dung. The inorganic fertilizer is one or more of nitrogen fertilizer, phosphate fertilizer, potash fertilizer and medium/micro-nutrient element fertilizer. The weight proportion of microbial inoculum to organic constituent to inorganic fertilizer is (5-25):(18-28):(47-77).

The invention relates to a complex microbial inoculum 707 and a preparation method and application thereof, and belongs to the technical field of biological materials of the application of plant biological regulators to crop planting. The preparation method of the complex microbial inoculum 707 comprises the following step of: performing the cultivation of one-level strains and second-level and third-level fermentation on strains comprising 45 to 55 percent of bacillus subtilis, 5 to 10 percent of bacillus licheniformis, 5 to 10 percent of bacillus megaterium, 5 to 10 percent of candida utilis, 5 to 10 percent of rhizobium japonicum, 5 to 10 percent of bacillus mucilaginosus and 5 to 10 percent of lactobacillus plantarum, so that the microbial content of fermentation liquor reaches 6-10*10<9> per milliliter to obtain the complex microbial inoculum 707. Through long-term field verification, the complex microbial inoculum 707 has the advantages of reasonable formula and stable effect, can promote the growth of crops, improve fruit quality, promote early ripening, increase yield, improve the utilization rate of fertilizers and improve the ecological environment of soil, and has certain effect on disease prevention and disease resistance.

The invention discloses a compound probiotics preparation for biological organic fertilizers and a preparation method thereof. The preparation method comprises the following steps: performing activated culture of 14 kinds of probiotics, including Pseudomona putida, Streptomyces violovariabilis, Rhizobium sp., Azotobacter chroococcum, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus subtilis, Bacillus weihenstephanensis, Bacillus mucilaginosus, Bacillus thuringiensis, Saccharomyces rouxii, Streptomyces griseus var. ferrugineus, and Streptomyces microflavus; performing mixed inoculation at a proportion of 1% to 5% to a 500 mL liquid seed culture medium; then performing amplification culture; and finally delivering the strains to a 500 mL fermentor and fermenting for 3 to 5 days under such conditions that the temperature ranges from 28 DEG C to 30 DEG C and the rotational speed is 200 rpm, to obtain a liquid-state compound probiotics preparation. The compound probiotics preparation for biological organic fertilizers, provided by the invention, plays important roles of taking advantage of microbial ecology, improving soil, promoting plant growth, improving the yield and disease resistance of crops and reducing the consumption of fertilizers.

The invention relates to a quick-acting multi-microorganism composite water flush fertilizer and a preparation method thereof. The quick-acting multi-microorganism composite water flush fertilizer is characterized by being prepared by mixing bacterial powder which is prepared by fermenting and culturing photosynthetic bacteria, pichiafermentanslodder, lactic acid bacteria, bifidobacterium, bacillus subtilis, bacillus licheniformis, brevibacillus laterosporus, bacillus megaterium, bacillus mucilaginosus, azotobocter chroococcum and actinomycetes. The density of the pichiafermentanslodder ACCC20319T in the quick-acting multi-microorganism composite water flush fertilizer is more than or equal to 2.0*10<8>cfu/g, and the densities of other bacteria are more than or equal to 2.0*10<9>cfu/g. The quick-acting multi-microorganism composite water flush fertilizer can improve the quality and yield of crops and shorten growth period of crops.

The invention relates to a liquid compound biological fertilizer with long shelf life as well as a preparation method and application thereof. The liquid compound biological bacterial fertilizer comprises the following components in parts by weight: 2-5 parts of microbial thalli, 80-130 parts of compound nutrients, 10-30 parts of thalli protective agents and 0.5-3.0 parts of additives, wherein the microbial thalli is metered in terms of the wet weight of deposition obtained after fermentation liquor is centrifugalized, the microbial thalli include bacillus thuringiensis, bacillus megaterium, bacillus mucilaginosus and bacillus amyloliquefaciens, and the total viable count achieves 5*10<8>-5*10<9>/mL. The liquid compound biological bacterial fertilizer disclosed by the invention can be used as a biological pesticide, can be directly applied to the spraying of plant surfaces or applied to seed soaking, root irrigation, and the like, has the effects of insect resistance, phosphate solubilization, potassium solubilization, organic matter solubilization, pest resistance, and the like and is long in shelf life.

The invention relates to a compound microbial fertilizer and a preparation method thereof. The compound microbial fertilizer is prepared by mixing the following raw materials: yeast fermentation powder residues, an organic fertilizer, urea, K2SO4, NH4H2PO4, clay and a compound microbial agent; and the compound microbial agent is formed by fermentation of bacillus mucilaginosus, bacillus megaterium, bacillus cereus, bacillus thuringiensis, azotobacter chroococcum, pichia farinose and streptomyces microflavus. The compound microbial fertilizer disclosed by the invention has a variety of microbes with the effects of nitrogen fixation, phosphorus dissolution and potassium dissolution, can increase the nitrogen content in soil, improves the utilization rate of the chemical fertilizer, and is favorable for improving the structure of the soil and improving the yield and quality of crops; and simultaneously, the microbes in the fertilizer can produce a variety of metabolites, and the metabolites can effectively resist diseases and insect pests.

The invention provides a microbial composition comprising acetobacter aceti, beauveria bassiana, azospirllum brasilense, nocardia cellulans, bacillus mucilaginosus, metarhizium anisopliae, trichoderma aureoviride and the like. The microbial composition can be stably combined in a microbial agent, is used for improving soil/land and especially can be used for effectively improving soil polluted by heavy metals.

The invention discloses a biological fertilizer, comprising, by weight, 65-80 parts of a concentrated nmolasses fermentation broth, 7-14 parts of potassium humate, 0.5-2 parts of a chelated microelement, 1-5 parts of microbial strains and 5-10 parts of a surfactant, wherein the chelated microelement is a mixture of EDTA-Cu, EDTA-Fe, EDTA-Mn and EDTA-Zn; and the microbial strains are composed of paenibacillus polymyxa, bacillus subtilis and bacillus mucilaginosus. The biological fertilizer is an environment-friendly type fertilizer, can improve soil environment and increase crop output, can be widely applied in plants particularly crops, has a very significant yield-increasing effect, and can increase the output of food crops, vegetables, melons and fruits by 10%-20%.

The invention relates to a liquid microbial fertilizer with nitrogen-fixing, phosphorus-dissolving and potassium-dissolving effects, and a preparation method thereof. The liquid microbial fertilizer consists of the following microbial fermentation liquids in parts by volume: 1 to 10 parts of azotobacter chroococcum, 1 to 30 parts of bacillus mucilaginosus, 1 to 10 parts of phosphorus-dissolving bacillus megatherium, 1 to 10 parts of bacillus amyloliquefaciens, 1 to 10 parts of bacillus subtilis, 1 to 5 parts of photosynthetic bacteria and 1 to 10 parts of streptomyces microflavus. The functional bacteria serve as main functional flora of the microbial fertilizer, so that the obtained product has remarkable effects of fixing nitrogen, dissolving phosphorus, dissolving potassium and resisting disease, can enhance the total metabolic activity of soil bacterial community and the diversity of the bacterial community, improve the soil micro-ecological environment, promote growth and reproduction of beneficial microorganisms and inhibit the growth of pathogenic bacteria, is an ideal fertilizer in green agriculture and organic agriculture, and has a wide prospect in agricultural sustainable development.

The invention discloses a method for producing a multifunctional bioorganic fertilizer through solid fermentation of botanical cake dregs by utilizing bacillus and application of the multifunctional bioorganic fertilizer, relating to a bioorganic fertilizer technology. In the method, the bacillus comprises Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus laterosporus and/or Bacillus mucilaginosus; and the botanical cake dregs comprise bean cake dregs, peanut cake dregs, sunflower cake dregs, sesame cake dregs, vegetable seed cake dregs and/or cotton seed cake dregs. The bioorganic fertilizer is mainly applied to biological prevention and treatment of plant diseases and promotion of plant growth. According to the invention, the production process is simple and practicable and has the advantages of less energy consumption, low cost, no toxicity and no harmless to people and livestock, no environment pollution and the like, is environmentally-friendly, safe and efficient, has huge potentials of industrial production and wide application, and has a wide market prospect.

The invention relates to a phosphorus and potassium decomposing biological organic fertilizer prepared from bacillus megatherium and Bacillus mucilaginosus. The fertilizer comprises the following components in percentage by weight: 30-40% of animal dung, 20-30% of kitchen waste, 15-25% of humic acid, 15-20% of crop straw, 1-2% of microelements and 4-6% of culture. A preparation method of the fertilizer comprises the following steps of: 1, inoculating bacillus megatherium to a nutritional gravy culture medium and the bacillus mucilaginosus to a silicate bacteria culture medium, and spraying a seed liquid after primary and secondary cultures onto a carrier to prepare conidial powder; 2, uniformly mixing 30-40% of animal dung, 20-30% of kitchen waste, 15-25% of turf, 15-20% of straw powder, 1% of potassium dihydrogen phosphate, 1% of zeolite powder, 1% of lime, 3-5% of boric sludge and 1% of gypsum; adjusting water content to 50-60% and stacking the materials into a fermentation tank; adding enzyme microorganisms and turning over mechanically; and fermenting for 3-4 weeks and drying, and crushing to obtain a finished product and packaging. According to the biological organic fertilizer, not only is the soil capacity enhanced, but also nutritional elements which can be absorbed and utilized are provided for crops.

The invention discloses a cultivation method for improving corn yield in saline-alkali land. The method comprises the following steps: irrigating the saline-alkali land for flooding for once in the middle of March, wherein the irrigation volume for per mu is 60-100 m<3>, airing for 1-1.5 months until the surface of earth is dry enough to allow people to step on, applying 1000kg-1500kg of miscellaneous manure or 50kg-55kg of compound fertilizer per mu, then performing rotary tillage for 1-2 times to mix soil and the fertilizer, pressing by using a harrow and preserving soil moisture, and leveling the surface of earth; treating corn by using a seed coating agent before the corn is seeded, wherein the seed coating agent comprises actinomycin, bacillus mucilaginosus, azospirillum, diatomite, spicy powder, heteroauxin, potassium sulphate, magnesium sulfate, zinc sulfate, an alpha-pimacol growth regulator, an EDTA-2Na chelating agent, humic acid and turf; mixing bactericide and insecticide with seeds for treating the seeds, namely, firstly adding the insecticide, and then adding the bactericide after piling for 3-6 hours; and immediately seeding after the seed mixing treatment is finished.

The invention discloses an environmentally-friendly efficient microbial compound fertilizer and a preparation method thereof. The environmentally-friendly efficient microbial compound fertilizer is prepared from chicken manure, cow dung, dried pond sludge powder, ground phosphate rock, edible fungi residues, furfural residues, a microbial agent, wheat bran, bean cakes, urea, ammonium nitrate, potassium humate, wheat straws, charcoal powder and traditional Chinese medicine residues, wherein the microbial agent is prepared from bacillus licheniformis, bacillus subtilis, bacillus mucilaginosus, bacillus megatherium, pseudomonas, bacillus thuringiensis, bacillus amyloliquefaciens and nitrogen-fixing bacteria. The edible fungi residues and the furfural residues are smashed and mixed with water, the microbial agent is added for fermentation, the Chinese traditional medicine residues are added to perform fermentation, then the rest of the raw materials are added, and the microbial compound fertilizer can be obtained by performing full mixing, granulation and drying. The raw materials used for the microbial compound fertilizer are all natural fertilizers and contain rich mineral substances, the damage of a soil acid-base structure and a nutritional ingredient structure caused by inorganic fertilizer application is avoided, and the environmentally-friendly efficient microbial compound fertilizer is greener and more environmentally-friendly.

The invention is suitable for the technical field of recycling organic wastes, and discloses a flora for recycling treatment of organic wastes. The flora for recycling treatment of organic wastes is efficiently compounded from a variety of bacteria such as bacillus subtilis, aspergillus niger, saccharomycete and soil flora, wherein the soil flora is extracted from the natural world and comprises three or more of bacillus megatherium, bacillus mucilaginosus, bacillus licheniformis, bacillus thuringiensis, bacillus laterosporus, bacillus mucilaginosus, streptomyces jingyangensis, mycorrhiza fungi, azotobacter vinelandii, photosynthetic bacteria, bacillus coagulans, aspergillus oryzae, and paecilomyces lilacinus. According to the flora for recycling treatment of organic wastes, disclosed by the invention, by virtue of mutual promotion, mutual coordination and combined action of a variety of beneficial microorganisms, the organic wastes are recycled, environmental pollution is reduced, and wastes are changed into valuables; and the flora for recycling treatment of organic wastes can be used for producing industrial fermentation products and improving the health and growth conditions of animals, and can also improve the current situation of serious soil hardening at present and improve soil quality, thereby promoting the rapid development of agricultural economics.

The invention relates to the field of soil fertilizer and provides a biological organic repairing agent for soil pollution and its application and preparation method. The biological organic repairing agent of the invention is composed of, by weight, 1.5-3 parts of eymogenic bacterium, 1-1.5 parts of wheat bran, 1.5-2 parts of molasses meal, 45-50 parts of ammonium humate, 45-50 parts of hydrolase dregs, 150-200 parts of soybean meal, 100-150 parts of humic acid powder, 80-100 parts of zeolite powder, 25-40 parts of a calcium magnesium phosphate fertilizer, 150-200 parts of cattle and sheep manure, 150-204 parts of plant straw, 25-40 parts of potassium humate, 1.5-3 parts of bacillus mucilaginosus, 1-1.5 parts of Gliocladium roseum, 1.5-2 parts of Streptomycesvinaceus-drappus, 1-1.5 parts of trichoderma spp, 1-1.5 parts of Paecilomyces lilacinus, 1-1.5 parts of bacillus subtilis and 1-1.5 parts of micrococcus. According to the invention, soil pollution control, improvement and nutrition are integrated.

The invention provides an organic material decomposition agent, a biological organic fertilizer containing tobacco wastes, and a production method and application method of the biological organic fertilizer, wherein the organic material decomposition agent comprises the following raw materials in parts by weight: 15-20 parts of bacillus subtilis, 10-15 parts of aspergillus oryzae, 10-15 parts of aspergillus niger, 10-15 parts of trichoderma viride, 10-15 parts of bacillus mucilaginosus, 10-15 parts of saccharomyces cerevisiae, 5 parts of bacillus thermophilus, 5 parts of arthrobacter nicotinovorans and 10-25 parts of actinomycetes. The organic material decomposition agent of the tobacco wastes provided by the invention can be applied to decomposing organic matters in the tobacco wastes, so that nitrogen, phosphorus and potassium elements are degraded into the form which can be absorbed and utilized by a plant, and meanwhile, the nicotine content in the tobacco can be reduced, so as to reduce the damage to a farmland after tobacco wastes are returned to the farmland, so that the organic material decomposition agent can play a role of fertilizing the soil after being returned to the farmland.

The invention discloses a saline-alkali soil biological improved fertilizer based on high-sulfur gangue. The saline-alkali soil biological improved fertilizer is prepared from the following components in parts by weight: 50-60 parts of high-sulfur gangue microbial fermentation product, 20-30 parts of compost cow manure or chicken manure, 1-2 parts of citric acid, 1-2 parts of oxalic acid, 2-4 parts of urea, 2-4 parts of ammonium sulfate, 2-4 parts of monoammonium phosphate, 1-3 parts of potassium sulphate, 2-5 parts of ferrous sulfate, 1-2 parts of zinc sulfate, 1-2 parts of manganese sulfate and 1-10 parts of bentonite, wherein the high-sulfur gangue microbial fermentation product is a product obtained by performing activating treatment on the high-sulfur gangue by a compound microorganism bacterium solution composed of thiobacillus thiooxidans, bacillus subtilis, bacillus megatherium, bacillus mucilaginosus and enterococcus faecalis. The resource utilization of the high-sulfur gangue is realized, the improvement of the inland saline land can be promoted, the crop yield can be increased and the entironment can be improved.