The invention discloses a GC
fingerprint spectrum detection method for
traditional Chinese medicine, and belongs to the field of
traditional Chinese medicine detection. The
traditional Chinese medicine is prepared from nine medicinal materials including
pericarpium citri reticulatae, thunberg fritillary
bulb, selfheal,
oroxylum indicum, platycodon grandiflorum, radix scrophulariae,
semen coicis, biond magnolia flower and divaricate
saposhnikovia root, and is used for treating the child
adenoid hypertrophy, and the method comprises the following steps: preparing a
test solution, adopting an HP-5
capillary column, performing analysis under the chromatographic conditions that the split ratio is 30: 1, temperature
programming is performed, the temperature of a sample inlet is 290 DEG C, and the temperature of a
detector is 300 DEG C, and recording a chromatogram for 075 min. A standard
fingerprint spectrum containing 19 common peaks is established by measuring a plurality of batches of samples, the attribution of each peak is defined, and five characteristic components such as
eucalyptol and beta-
bisabolol are identified. The method is good in stability and high in reproducibility, the constructed
fingerprint spectrum is high in characteristic, the method can be used for quality consistency evaluation and production
process control of traditional Chinese
medicine, and safety and effectiveness of clinical medication are guaranteed.