The invention discloses a
facultative anaerobic
bacillus altitudinis strain and an application thereof in tobacco, the
facultative anaerobic
bacillus altitudinis strain ZY02-1 is classified and named as
Bacillus altitudinis, the strain is preserved in the China General
Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33916. The invention further discloses a preparation method of the
facultative anaerobic
bacillus altitudinis strain. The strain is a facultative
anaerobic bacterium, can be fermented under two conditions of an aerobic environment and an anaerobic environment, can promote combined
nicotine to be converted into free
nicotine in a low-
oxygen environment, increases the
nicotine content of tobacco stems, effectively accelerates
decomposition of
aroma precursors such as polyphenols and terpenes in the tobacco stems, effectively increases
aroma substances of the tobacco stems, and has the advantages of high yield, high yield and the like before re-
drying. The bacillus altitudinis ZY02-1 is applied to
moisture regaining tobacco stems for stacking
fermentation, offensive
odor of the fermented tobacco stems is reduced,
aroma quality, aroma quantity and concentration are improved, nicotine, megastigmatrienone,
geraniol and alpha-
pinene are remarkably increased, and aroma components such as alpha-cedrene and
ocimene are added.