36 results about "Odorant-binding protein" patented technology

The invention discloses an apparatus and a method for detecting a combination process of odorant-binding protein and pheromone. The apparatus comprises a sample introduction system, a measure transition system, a counter electrode, a reference electrode, a working electrode, a measure groove, an oscillator, a liquid-outlet peristaltic pump, a waste liquid groove and a computer; the sample introduction system consists of a first peristaltic pump, a second peristaltic pump, a third peristaltic pump, a first injector, a second injector, a third injector and a controller; and the measure transition system consists of a measure circuit, a high-resistance electrometer and a measure circuit. The method comprises preparing honeybee pheromone isoamyl-acetate standard solutions with different concentration, an apis-cerana-ceraca odorant-binding protein (Acer-ASP2) standard solution, and mixed solutions of apis-cerana-ceraca odorant-binding protein (Acer-ASP2) and honeybee pheromone isoamyl acetate and with different concentration, and detecting variation of electrochemical impedance of the three kinds of solutions along with time, so as to obtain corresponding electrochemical impedance spectrograms, and further to obtain the combination of odorant-binding protein and pheromone.

The present invention discloses a method of expressing recombinant odorant binding protein (OBP) of insect in prokaryotic cell, and vector containing target nucleic acid sequence is transformed to competent prokaryotic cell. The present invention also discloses new OBP-coding nucleic acid sequence obtained from Locusta migratoria antenna.

The invention discloses a method for detecting expression of an odorant-binding protein gene OBP6 of Italian bees by using fluorescent RT-PCR technology, and particularly relates to the technical field of biology. The method for detecting the expression of the odorant-binding protein gene OBP6 of the Italian bees by using the fluorescent RT-PCR technology comprises the following steps that (1), OBP6 genes are detected by using primers; (2), total RNA extraction is performed; (3), cDNA synthesis is performed; (4), real-time fluorescent PCR is performed; and (5), the relative expression quantityof OBP6 of the Italian bees is calculated before and after benomyl treatment. The real-time fluorescent RT-PCR adopted by the method for detecting the expression of the odorant-binding protein gene OBP6 of the Italian bees by using the fluorescent RT-PCR technology is compared with conventional PCR, and the real-time fluorescent PCR technology collects fluorescent signals by automated instruments, the subjectivity of visual judgment is avoided, and the sensitivity is further improved; and at the same time, the real-time fluorescent RT-PCR technology reacts in a fully closed mode without post-treatment of PCR, pollution is avoided, and the reliability and repeatability of the results are ensured. At the same time, the following tedious steps such as electrophoresis and quantitative scanning in routine PCR are eliminated, and the experiment time is greatly shortened.

The invention discloses a citrus fruit fly odorant binding protein-based attractant screening method belonging to the technical field of bioengineering. The citrus fruit fly odorant binding protein-based attractant screening method comprises the following steps of: collecting the total RNA (Ribonucleic Acid) of antennae of a citrus fruit fly; obtaining the overall length of a citrus fruit fly odorant binding protein through RT-PCR (Reverse Transcription-Polymerase Chain Reaction); constructing a prokaryotic expression vector of the citrus fruit fly odorant binding protein; inducing the expression of a citrus fruit fly recombinant odorant binding protein through IPTG (isopropyl-beta-d-thiogalactoside), and purifying the citrus fruit fly recombinant odorant binding protein through a nickel sepharose gel affinity column; obtaining a conjugation reaction spectrum of the citrus fruit fly recombinant odorant binding protein and a host fruit odor volatile matter through a competitive fluorescence combing method, wherein a dissociation constant (KD) is lower than below 10 mu mol / L host fruit smell; and the IC 50 value of fluorescence competition is less than 30 mu mol / L, determining a host fruit smell attractant suitable for the citrus fruit fly. The invention provides a new strategy for screening and designing a citrus fruit fly odorant host fruit smell odor information attractant formula.