8 results about "Odorant-binding protein" patented technology

The invention provides an odorant binding protein sourced from cicada and application of the odorant binding protein in prevention and treatment of rice dwarf disease. The amino acid sequence of the odorant binding protein is SEQ ID NO: 1. According to the application disclosed by the invention, a fluorescent competitive binding experiment finds that the odorant binding protein NcinOBP8 of the black tail leafhopper and a volatile matter released by rice induced by a rice dwarf virus have high affinity; the RNAi experiment shows that the rice volatile matter induced by the rice dwarf virus has an attraction effect on the black-tail leafhopper nymphs injected with dsEGFP, but after the NcinOBP8 is silenced, the rice volatile matter does not attract the black-tail leafhopper nymphs after 2 hours. Therefore, the silent cicada NcinOBP8 can influence the perception of the cicada on the rice volatile matters.

The invention discloses an odorant binding protein OBP7 gene and application of dsRNA of the odorant binding protein OBP7 gene in prevention and treatment of solenopsis invicta. The invention proves that SiOPB7 can identify undecanal so as to trigger the corpse stacking behavior of solenopsis invicta, specifically, the corpse stacking behavior of solenopsis invicta triggered by undecanal is inhibited by feeding worker solenopsis invicta with dsOBP7, the pathogen transmission risk in a solenopsis invicta nest is increased, the social sexual behavior is interfered, the group adaptability is reduced, and the effect of controlling solenopsis invicta is achieved. The invention provides a theoretical basis for developing a preparation for preventing and controlling the solenopsis invicta by taking the SiOBP7 gene / protein as a target, and has important significance on green prevention and control of the solenopsis invicta.

The invention provides an aphid pest attractant. According to the aphid pest attractant, benzyl isobutyrate is used as a myzus persicae attractant. The invention finds that benzyl isobutyrate can be specifically bound with odorant binding protein of myzus persicae on a molecular level, so that the benzyl isobutyrate can be used as an effective active ingredient of the myzus persicae attractant. Benzyl isobutyrate is adopted as an active component of the attractant, so that the trend response to myzus persicae can be remarkably enhanced, and the attraction efficiency can be improved. Meanwhile, the compound is clear in structure, controllable in source, convenient to prepare and apply on a large scale, beneficial to reducing the use amount of chemical pesticides and reducing the risk of environmental pollution, suitable for green prevention and control of myzus persicae and good in application prospect.

The invention discloses a tomato leaf miner odorant binding protein TabsPBP1.3 and application of a coding gene thereof, and belongs to the technical field of molecular biology. The PBP1.3 gene is cloned from an important invasive pest tomato leaf miner gene for the first time, the amino acid sequence of the tomato leaf miner odorant binding protein is shown as SEQ ID NO.1, and the coding gene of the odorant binding protein is shown as SEQ ID NO.2. The protein and the gene provided by the invention lay a molecular foundation for analyzing the olfactory molecular mechanism of the phyllocnistis citrella, and can be used for identifying active components of reverse verification odor compounds; and a potential target gene is provided for developing olfactory behavior regulators, such as screening of attractants or repellents.

According to the odorant binding protein for the black-tailed leafhopper and the application of the odorant binding protein, by interfering the expression quantity of the binding protein in the black-tailed leafhopper, the perceptual ability of the black-tailed leafhopper to volatile matters induced by rice dwarf viruses can be effectively inhibited, so that the effect of preventing and treating the rice dwarf viruses is achieved. The amino acid sequence of the odorant binding protein is SEQ ID NO: 1. According to the application disclosed by the invention, a fluorescent competitive binding experiment finds that the odorant binding protein NcinOBP7 of the black tail leafhopper and a volatile matter released by rice induced by a rice dwarf virus have high affinity; an RNAi experiment shows that the rice volatile matter induced by the rice dwarf virus has an attraction effect on the black-tail leafhopper nymphs injected with dsEGFP, but after the NcinOBP7 is silenced, the rice volatile matter does not attract the black-tail leafhopper nymphs any more. Therefore, the silence of the black-tail leafhopper NcinOBP7 can influence the perception of the black-tail leafhopper on the rice volatile matters, and subsequently speculates to inhibit the feeding of the black-tail leafhopper on the virus-infected rice and influence the transmission of viruses.

The present application relates to the field of agricultural biotechnology, in particular to a tomato leafminer odor binding protein, an encoding gene thereof and application thereof. The amino acid sequence of the tomato leafminer odor binding protein is shown as SEQ ID No: 1. The tomato leafminer odor binding protein TabsOBP11 provided in the present application can bind with host plant volatiles indole, and indole has an obvious repelling effect on tomato leafminer larvae, thus the present application provides a reliable basis for preventing and treating tomato leafminer through biotechnological means.

Provided herein are compositions comprising an odor binding protein for non-aerosol formulations intended, to maintain hair and / or skin health of an individual and prolong time between application of such compositions. In some embodiments, the compositions may remove malodors. Also, provided herein are methods of making and using compositions described herein for non-aerosol formulations for maintaining hair and / or skin health.

The invention discloses application of a tomato leaf miner odorant binding protein and a coding gene thereof in screening behavior regulation reagents, and belongs to the technical field of molecular biology. The tomato leaf miner odorant binding protein provided by the invention comprises TabsPBP1.1 and TabsPBP3, the PBP1.1 gene and the PBP3 gene are cloned from an important invasive pest tomato leaf miner gene for the first time, and a recombinant protein is obtained according to the genes and is used for functional verification of the odorant binding protein. Results prove that the odorant binding protein of the phyllocnistis solani provided by the invention plays an important role in specific recognition of sex pheromone of the phyllocnistis solani. According to the technical scheme provided by the invention, a molecular foundation is laid for analyzing the olfaction molecular mechanism of the tomato leaf miner, and a potential target gene is provided for developing and screening a novel green olfaction behavior regulating agent.