36 results about "Odorant-binding protein" patented technology

The invention discloses a method for detecting the self-mutilation rate of insects by using an odorant binding protein 15 gene. The invention provides an application of a substance for detecting the expression level of an odorant binding protein 15 coding gene in insect populations fed with different substances in detection of the self-mutilation rate of the insect populations fed with different substances; and the amino acid sequence of an odorant binding protein 15 is sequence 2 in a sequence table. According to the method disclosed by the invention, experiments prove that the detection method provided by the invention can be used for detecting the self-mutilation rate of the insects, in particular to the self-mutilation rate of arma chinensis with different nutrient sources; and the detection of the self-mutilation rate of the insects by the detection method disclosed by the invention only requires 2-3 days. However, the use of a traditional biological method requires about 30-60 days. The detection method disclosed by the invention has the advantages of wide range of involved material sources, easiness in purchase, simplicity in operation, and time-saving and labor-saving properties, and is suitable for popularization and application in commodity inspection and detection of insects.

The invention discloses an apparatus and a method for detecting a combination process of odorant-binding protein and pheromone. The apparatus comprises a sample introduction system, a measure transition system, a counter electrode, a reference electrode, a working electrode, a measure groove, an oscillator, a liquid-outlet peristaltic pump, a waste liquid groove and a computer; the sample introduction system consists of a first peristaltic pump, a second peristaltic pump, a third peristaltic pump, a first injector, a second injector, a third injector and a controller; and the measure transition system consists of a measure circuit, a high-resistance electrometer and a measure circuit. The method comprises preparing honeybee pheromone isoamyl-acetate standard solutions with different concentration, an apis-cerana-ceraca odorant-binding protein (Acer-ASP2) standard solution, and mixed solutions of apis-cerana-ceraca odorant-binding protein (Acer-ASP2) and honeybee pheromone isoamyl acetate and with different concentration, and detecting variation of electrochemical impedance of the three kinds of solutions along with time, so as to obtain corresponding electrochemical impedance spectrograms, and further to obtain the combination of odorant-binding protein and pheromone.

The invention relates to a separated vegetable leafminer odorant-binding protein, its encoding protein and an application thereof. The protein can bind to odors matters of 4-ethyl benzaldehyde, azulene, 3,4-dimethyl acetophenone and p-ethylacetophenone, and combination of the protein and the odors matters can be used for preventing and treating vegetable leafminer.

The invention discloses a preparation method of a nano cup array biosensor for ionone detection. The method comprises the steps that a nanoscale cup-shaped structure is constructed by a nano impressing technology; gold nanoparticles are deposited on a side wall of a nano cup; odorant binding protein Acer-ASP2 is fixed on the side wall of the nano cup by electrostatic adsorption of the gold nanoparticles; during detection, a target molecule ionone is specifically bound with the odorant binding protein to result in change of an optical refraction characteristic of the side wall of the nano cup; and a spectrum of transmission light passing through the biosensor is changed correspondingly, so that the detection of the target molecule ionone is realized. Compared with the traditional ionone detection means, the nano cup array biosensor avoids a complicated light path required by optical detection and is simple, convenient and quick to operate based on the advantages of high sensitivity and specificity of the optical detection means.

The present invention discloses a method of expressing recombinant odorant binding protein (OBP) of insect in prokaryotic cell, and vector containing target nucleic acid sequence is transformed to competent prokaryotic cell. The present invention also discloses new OBP-coding nucleic acid sequence obtained from Locusta migratoria antenna.

The invention discloses a method for detecting expression of an odorant-binding protein gene OBP6 of Italian bees by using fluorescent RT-PCR technology, and particularly relates to the technical field of biology. The method for detecting the expression of the odorant-binding protein gene OBP6 of the Italian bees by using the fluorescent RT-PCR technology comprises the following steps that (1), OBP6 genes are detected by using primers; (2), total RNA extraction is performed; (3), cDNA synthesis is performed; (4), real-time fluorescent PCR is performed; and (5), the relative expression quantityof OBP6 of the Italian bees is calculated before and after benomyl treatment. The real-time fluorescent RT-PCR adopted by the method for detecting the expression of the odorant-binding protein gene OBP6 of the Italian bees by using the fluorescent RT-PCR technology is compared with conventional PCR, and the real-time fluorescent PCR technology collects fluorescent signals by automated instruments, the subjectivity of visual judgment is avoided, and the sensitivity is further improved; and at the same time, the real-time fluorescent RT-PCR technology reacts in a fully closed mode without post-treatment of PCR, pollution is avoided, and the reliability and repeatability of the results are ensured. At the same time, the following tedious steps such as electrophoresis and quantitative scanning in routine PCR are eliminated, and the experiment time is greatly shortened.

The invention discloses a tea geometrid odorant binding protein-based plant attractant screening method and belongs to the technical field of bioengineering. The tea geometrid odorant binding protein-based plant attractant screening method comprises the following processes of collecting tea geometrid antenna total RNA, acquiring total length of a tea geometrid odorant binding protein by RT-PCR, constructing a prokaryotic expression vector of the tea geometrid odorant binding protein, inducing the expression of the recombinant tea geometrid odorant binding protein by IPTG, carrying out the purification of the recombinant tea geometrid odorant binding protein by nickel sepharose gel affinity column, acquiring a binding reaction spectrum of the recombinant tea geometrid odorant binding protein and tea leaf smell volatiles by a competitive fluorescent combination method, and determining that the tea leaf smell volatile reducing relative fluorescence intensity of 1-NPN to less than 50% and having the binding constant of 13-45 micromoles per liter is the plant attractant of tea geometrid. The tea geometrid odorant binding protein-based plant attractant screening method provides a novel means for screening and designing a formula of a plant tea geometrid smell information attractant.

The invention discloses a citrus fruit fly odorant binding protein-based attractant screening method belonging to the technical field of bioengineering. The citrus fruit fly odorant binding protein-based attractant screening method comprises the following steps of: collecting the total RNA (Ribonucleic Acid) of antennae of a citrus fruit fly; obtaining the overall length of a citrus fruit fly odorant binding protein through RT-PCR (Reverse Transcription-Polymerase Chain Reaction); constructing a prokaryotic expression vector of the citrus fruit fly odorant binding protein; inducing the expression of a citrus fruit fly recombinant odorant binding protein through IPTG (isopropyl-beta-d-thiogalactoside), and purifying the citrus fruit fly recombinant odorant binding protein through a nickel sepharose gel affinity column; obtaining a conjugation reaction spectrum of the citrus fruit fly recombinant odorant binding protein and a host fruit odor volatile matter through a competitive fluorescence combing method, wherein a dissociation constant (KD) is lower than below 10 mu mol / L host fruit smell; and the IC 50 value of fluorescence competition is less than 30 mu mol / L, determining a host fruit smell attractant suitable for the citrus fruit fly. The invention provides a new strategy for screening and designing a citrus fruit fly odorant host fruit smell odor information attractant formula.