154 results about "Ionone" patented technology

The invention discloses microchannel reaction equipment and a method for continuously synthesizing beta-ionone. The micro-channel reaction equipment comprises a low-temperature pipe section and a high-temperature pipe section communicated with the tail end of the low-temperature pipe section; the low-temperature pipe section and the high-temperature pipe section respectively comprise two wavy reaction pipes which are arranged in a staggered manner; the two reaction tubes of the low-temperature tube section accord with the trend of a cosine function curve along the axial extension direction, and the two reaction tubes of the high-temperature tube section accord with the trend of a sine function curve along the axial extension direction. By adopting the micro-channel reaction equipment to continuously react and synthesize beta-ionone, the generation of other isomers and tar substances can be controlled, so that the reaction is mainly carried out towards the generation direction of the beta-ionone, and the subsequent separation operation does not need rectification operation with a high theoretical plate number; therefore, a high-purity beta-ionone product can be obtained.

The invention belongs to the field of synthesis of organic matters, and particularly relates to a synthesis method of 4-oxo-beta-ionone, which comprises the following steps: S1, dissolving beta-ionone in a good solvent, and introducing sulfur dioxide; s2, adding water and a catalyst into the solvent, and then adding an oxidizing agent; s3, removing a water phase, and adding an alkaline solution; s4, separating a water phase, recovering the solvent, and distilling to obtain 4-oxo-beta-ionone; according to the method, the beta-ionone is oxidized into the 4-oxo-beta-ionone by using the oxidizing agent, and the sulfur dioxide is introduced to protect conjugated double bonds on a six-membered ring in the beta-ionone before the oxidizing agent is added, so that the oxidation selectivity of the oxidizing agent is improved, and the oxidation efficiency is high.

The invention belongs to the technical field of perfume synthesis, and particularly relates to a method for efficiently preparing chiral oxo-ionol by an enzymic method, which comprises the following steps: putting a substrate containing oxo-ionone and an auxiliary substrate into a buffer solution, and stirring at room temperature until complete dissolution to obtain a substrate solution; the method comprises the following steps: dispersing carbonyl reductase ChKRED20 or a mutant thereof and coenzyme into a substrate solution, and carrying out heat preservation and stirring; and after the reaction is finished, adding water to dilute, extracting with ethyl acetate, washing with water, drying, and concentrating the ethyl acetate to obtain the chiral oxo-ionol. The enzyme catalysis method disclosed by the invention is mild in reaction condition and high in selectivity, and a target product can be obtained at high yield at room temperature and normal pressure. The coenzyme is regenerated by adopting a substrate coupling method, the use amount of the coenzyme is reduced, and a substrate coupling strategy that an auxiliary substrate is used as a substrate is adopted, so that a reaction system is simple, products are easy to separate, and the cost is reduced. According to the technical scheme, the conversion rate is greater than 99%, the ee value of the product reaches 99% or above, and the industrial application value is achieved.

The invention relates to the technical field of carotenoid cleavage dioxygenase, and discloses a carotenoid cleavage dioxygenase mutant and application thereof. In order to improve the efficiency of converting carotenoid in tobacco into aroma molecule beta-ionone, specific mutation of characteristic sites is carried out on carotenoid cleavage dioxygenase (PhCCD1) from petunia, that is, serine (S) at the 428 site of the carotenoid cleavage dioxygenase is mutated into cysteine (C) or tyrosine (Y) to obtain a mutant S428C or S428Y, and the mutant S428C or S428Y is converted into beta-ionone. The yield of the beta-ionone converted from the carotenoid is greatly improved. Experiments prove that the yields of beta-ionone of S428C and S428Y respectively reach 54.2 mg / L and 50.6 mg / L, which are respectively increased by 69.7% and 58.5% compared with those of a wild strain WT.

The invention discloses bacillus subtilis and application thereof, and belongs to the technical field of microorganisms. According to the invention, a degradation strain capable of improving cellulose and lignin in cigar tobacco leaves is screened out, and after the strain is fermented, the activity of supernate cellulase, the activity of supernate laccase, the activity of supernate manganese peroxidase and the activity of supernate lignin peroxidase are respectively 11.21 U / mL, 26.82 U / mL, 6.31 U / mL and 3.78 U / mL. After fermentation optimization, the activity of cellulase, the activity of laccase, the activity of manganese peroxidase and the activity of lignin peroxide are 30.29 U / mL, 42.69 U / mL, 8.96 U / mL and 5.74 U / mL respectively. The strain Y-C4 is applied to tobacco leaf fermentation, fermented tobacco leaves generate substances including solanesol, acetophenone, geranyl acetone, beta-ionone, myristic aldehyde and the like, and the strain has an excellent application prospect in tobacco leaf fermentation.

The utility model belongs to the technical field of purification devices, and discloses an efficient ionone purification device which comprises a purification tank and a heating pad, a beaker is attached to the top end of the heating pad, a cavity is formed in the beaker, a condensation pipe is arranged in the cavity of the beaker in a penetrating mode, and a liquid outlet is formed in the outer side of the condensation pipe in a penetrating mode. A supporting sliding ring is connected to the outer side of the liquid outlet in a sleeving mode, a collecting box is arranged at the bottom end of the liquid outlet, a filter screen is slidably connected into the collecting box, and a circular groove is formed in the filter screen. And after the container is used, substances are adhered to the inner wall and are difficult to clean manually.

The invention discloses osmanthus transcription factors OfbHLH10 and OfWRKY78 for regulating synthesis of a flower fragrance component beta-ionone as well as a coding gene and application of the osmanthus transcription factors OfbHLH10 and OfWRKY78, and belongs to the field of plant molecular biology. The amino acid sequence of the osmanthus fragrans transcription factor OfbHLH10 provided by the invention is as shown in SEQ ID NO. 2, and the amino acid sequence of the transcription factor OfWRKY78 interacting with the osmanthus fragrans transcription factor OfbHLH10 is as shown in SEQ ID NO. 4. According to the invention, carriers constructed by CDs of OfWRKY78 and a promoter of OfCCD4 are jointly injected into Ben's tobacco leaves, a dual-luciferase experiment is carried out, and a result shows that OfbHLH10 has a promoting effect on expression of a synthetase gene OfCCD4 of beta-ionone, OfWRKY78 has an inhibiting effect on expression of OfCCD4, and OfbHLH10 and OfWRKY78 interact with each other and has an antagonistic effect on expression of OfCCD4. The gene provided by the invention can effectively regulate and control the content of beta-ionone in osmanthus fragrans fragrance, and has a good application prospect.

The invention provides an essence with a jasmine tea flavor. Comprising leaf alcohol, linalool, phenethyl alcohol, alpha-amyl cinnamic aldehyde, hexanal, benzyl acetate, jasmine absolute oil, neroli oil, leaf alcohol acetate, indole, geraniol, geranyl acetate, linalyl acetate, methyl dihydrojasmonate, farnerol, alpha terpilenol, damascenone B, ionone B, benzyl acetate, benzyl alcohol, leaf alcohol benzoate and ethyl maltol. The compound is one or more of methyl anthranilate, methyl benzoate and methyl salicylate. The jasmine tea beverage food added with the jasmine tea flavor essence disclosed by the invention can effectively modify and improve the flavor of the jasmine tea beverage, increase the tea fragrance and flower fragrance of the jasmine tea beverage, and enable the fragrance and taste to be richer and fuller.

The invention discloses tobacco carotenoid monooxygenase BCM for catalytic synthesis of beta-ionone and application of the tobacco carotenoid monooxygenase BCM in tobacco leaf quality improvement, and belongs to the technical field of genetic engineering. The amino acid sequences of the tobacco carotenoid monooxygenase BCM are as shown in SEQ ID NO. 1 to SEQ ID NO. 10. The carotenoid monooxygenase BCM disclosed by the invention has relatively good enzymatic activity and can be applied to catalyzing beta-carotene to generate beta-ionone, and the production capacity of an expression strain disclosed by the invention is stable and sustainable; when the recombinant engineering bacterium expressing the BCM is applied to tobacco leaf fermentation, the content of beta-ionone in tobacco leaves can be increased, the smoking quality of the tobacco leaves is improved, and the recombinant engineering bacterium shows a wide application prospect in the fields of tobacco leaf fermentation and quality improvement and is expected to promote transformation and upgrading of the tobacco industry.

An antifungal composition including a first essential oil, and a second essential oil, where the composition is configured to reduce disease onset, pathogen spread, or a combination thereof. Further, an antifungal compound including one or more aroma compounds selected from 4-(4-hydroxyphenyl)-2-butanone, furaneol, ethyl methylphenylglycidate, ethyl hexanoate, ethyl butanoate, beta-ionone, alpha-ionone, (R)-(+)-limonene, (S)-(-)-limonene, cis-3-Hexen-1-ol, nerol, linalool, 2-heptanone, alpha-terpineol, beta-damascenone, β-caryophyllene, ethyl 2-methylbutanoate, β-myrcene, gamma-octalactone, alpha-humulene, nerolidol, (E,E)-2,4-decadienal, (E,E)-α-farnesene, vanillin, phenylethyl acetate, citral, 3-methylbutan-1-ol, or a combination thereof.

The invention provides a method for regulating and controlling aroma of grape fruits through an exogenous plant growth regulator, which comprises the following steps of: spraying three exogenous plant hormones, namely methyl jasmonate, phenylalanine and strigolactones, on 'Hanxiangmi' grape fruits with strong rose aroma, and analyzing the aroma composition and content of the treated grape fruits. The result shows that the rose fragrance intensity of the'Hanxiangmi 'grapes is enhanced after the jasmonic acid methyl ester treatment; after strigolactones treatment, components such as alpha-terpilenol, beta-ionone, nonanoic acid, geraniol and the like in grape fruits are prominent, so that the treated grapes are converted into mixed fragrance type grapes with flower fragrance, fruit fragrance and grass fragrance, and the fragrance layers are rich; and the treatment effect of phenylalanine is not obvious. The invention provides a reference for the research of strigolactone in fruit quality.

The invention discloses a transcription factor OfMYB187 for regulating synthesis of beta-ionone of a sweet osmanthus fragrance component, an interaction protein OfCLpP of the transcription factor OfMYB187 as well as coding genes and application of the transcription factor OfMYB187 and the interaction protein OfCLpP, and belongs to the field of plant molecular biology. The amino acid sequence of the transcription factor OfMYB187 provided by the invention is as shown in SEQ ID NO. 2, and the amino acid sequence of the interaction protein OfCLpP of the transcription factor OfMYB187 is as shown in SEQ ID NO. 4. According to the application disclosed by the invention, a carrier constructed by promoters of OfMYB187, OfCLpP and OfCCD4 is jointly injected into a Ben's tobacco leaf, firefly and renilla determination is carried out, and a result shows that the OfMYB187 has an inhibition effect on a synthetase gene OfCCD4 of beta-ionone, but after interaction of the OfMYB187 and the OfCLpP, the OfMYB187 protein can be hydrolyzed, and the inhibition effect of the OfMYB187 protein on the OfCCD4 is relieved. The gene provided by the invention can directly or indirectly regulate and control the content of beta-ionone in osmanthus fragrans fragrance, and has a good application prospect.

The present invention addresses the problem of improving the change in smell of stearyl glycyrrhetinate,. This composition is characterized by containing components (A)–(C). (A) Stearyl glycyrrhetinate, (B) niacinamide or tranexamic acid, and (C) at least one compound selected from the group that consists of anisyl alcohol, farnesol, eugenol, citronellol, α-pinene, 1-hexanol (hexen-1-ol), linalool, cedryl acetate, Iso E Super, Sandalore, Ambroxan, γ-terpinene, ambrettolide, β-ionone, borneol, isobutyl quinoline, citral, guaiacol, patchouli alcohol, cedrol, ethyl vanillin, maltol, linalyl acetate, α-terpineol, benzyl benzoate, damascenone, anisaldehyde, benzaldehyde, Magnolan, Mayol, phenylacetaldehyde dimethyl acetal, nerol, dimethyl benzyl carbinol, Bacdanol, methyl anthranilate, Muscenone, benzyl acetate, phenylethyl alcohol, phenylethyl acetate, and heliotropine.

The invention discloses a high-beta-ionone tobacco leaf as well as a preparation method and application thereof. According to the high beta-ionone tobacco leaf overexpression gene NtCCD4a, the nucleotide sequence of the gene NtCCD4a comprises a sequence as shown in SEQ ID NO. 1, and the nucleotide sequence of the gene NtCCD4a comprises a sequence as shown in SEQ ID NO. 2. The content of beta-ionone in the transgenic tobacco leaf obtained by the method is increased by more than 5 times compared with that of a wild tobacco plant, and the transgenic tobacco leaf has a great application prospect in the aspect of cultivating tobacco with high beta-ionone content.

The invention relates to the technical field of microcapsules, in particular to watermelon essence and a preparation method thereof. The watermelon essence is composed of a composite core material and a wall material, the composite core material comprises a watermelon aldehyde pre-clathration core material, cis-3-hexenol, trans-2-hexenal, beta-ionone, leaf acetate and other flavor substances, and the wall material is coated with a pre-clathration layer and a composite nano layer from inside to outside in sequence. The three-stage process of reducing high-temperature volatilization loss in the preparation process through watermelon aldehyde pre-clathration, realizing precise cladding through nano-emulsification and preparing micro powder through spray drying and curing is adopted, so that the three problems of thermal instability, poor slow release property and high cost existing in the field of watermelon essence for a long time are solved, and the watermelon essence has the advantages of high embedding rate, uniform particle size distribution, low cost and the like. The effects of resisting high-temperature processing and prolonging the slow release property are achieved.

The invention discloses a composition for enhancing Chinese chestnut fragrance of a tea beverage as well as a preparation method and application of the composition, and belongs to the technical field of food additives. The composition is prepared from the following raw materials in parts by weight: 0.1 to 0.9 part of furanone, 0.01 to 0.2 part of linalool, 0.001 to 0.5 part of 2, 5-dimethylpyrazine, 0.02 to 0.1 part of geraniol, 0.03 to 0.05 part of beta-ionone and 0.01 to 0.25 part of geranyl acetone. According to the invention, through a unique ratio design, the scorched nut smell of pyrazine compounds, the rich caramel sweet taste of furanone, the stir-fried smoke and fire smell of 2-acetylthiazole and the freshness of geraniol and other terpene alcohols are ingeniously fused, and the complex level of Chinese chestnut fragrance is accurately reproduced. According to the present invention, the edible-grade propylene glycol is adopted as the solvent, such that the solubility, the dispersibility and the stability of the aroma composition in the tea beverage can be effectively improved, the persistence and the uniformity of the aroma release can be significantly improved, the shelf life of the tea beverage product can be easily prolonged, and the market competitiveness of the product can be enhanced.

The invention discloses a tobacco carotenoid dioxygenase NtCCD10 mutant as well as a genetically engineered bacterium and application thereof, and belongs to the technical field of oxidation aroma production of carrots. The thermal stability of the mutant NtCCD10-G189P-K279R disclosed by the invention is obviously improved compared with that of the NtCCD10, and the residual enzyme activity of the NtCCD10-G189P-K279R is 1.25 times that of the NtCCD10 under the condition that the mutant NtCCD10-G189P-K279R is incubated for 30 minutes at the temperature of 50 DEG C. A recombinant strain is constructed based on different carotenoid dioxygenase, and beta-carotene can be cracked to produce an aroma substance beta-ionone; the beta-carotene degradation rate of the constructed recombinant strain reaches 54.59% or above within 24 h, and the yield of beta-ionone reaches 20.721 mg / L or above; after the recombinant strain is sprayed to fermented tobacco shreds, the tobacco shred fragrance can be obviously improved, and the tobacco leaf quality is improved.

The present invention discloses a method for biocatalytic conversion of β-ionol to prepare dihydro-β-ionone, belonging to the technical field of bioengineering. In the present invention, β-ionol is used as a substrate, and short-chain alcohol dehydrogenase NaSDR and enoate reductase AaDBR1 are added for reaction. The reaction temperature is 25-60 °C, the pH is 4.0-11.0, and the reaction time is 0.5-3 h to obtain β-ionone. The present invention uses short-chain alcohol dehydrogenase and enoate reductase for cascade reaction, establishes a process for in vitro one-pot biopreparation of dihydro-β-ionone using β-ionol as a substrate, and realizes the regeneration and self-circulation of NADPH, thus creating a new method for highly efficient and low-cost biosynthesis of dihydro-β-ionone.

The application belongs to the technical field of biological medicine, and particularly relates to a lactobacillus paracasei and application thereof. The lactobacillus paracasei ZY101 is preserved in the China General Microbiological Culture Collection Center, has a preservation number of CGMCC No. 32360, a preservation date of October 25, 2024, and an address of No. 3, Beichen West Road, Chaoyang District, Beijing. Compared with a commercial bacillus acidophilus, the apricot vinegar prepared by using the lactobacillus paracasei ZY101 has new substances including linalool, alpha-farnesene, 2-phenylacetic acid ethyl ester and geranylgeraniol, and further promotes the aroma of the apricot vinegar. Compared with the commercial bacillus acidophilus, the existing substances are obviously improved, and specifically include ethyl acetate, isoamyl acetate, ethyl octanoate, ethyl hexanoate, acetoin, methyl palmitate, gamma-deca-lactone, ethyl dodecanoate, delta-deca-lactone, trans-nerolidol and dihydro-beta-ionone.

The invention relates to the technical field of genetic engineering, and discloses a genetically engineered bacterium for producing beta-ionone at high yield and application of the genetically engineered bacterium. A synthetic biological plasmid modularization technology is adopted, coding genes of carotenoid cleavage dioxygenase NtCCD1 in tobacco are excavated, and specific soluble protein tags including a thioredoxin tag, a maltose binding protein tag and a glutathione transferase tag are screened, so that the content of the carotenoid cleavage dioxygenase NtCCD1 in the tobacco is determined. The NtCCD1 is subjected to single-point mutation modification of mutation of 431st-site histidine into tyrosine, lysine or glutamine, and the fermentation enzyme and the gene engineering bacterium which can efficiently catalyze splitting decomposition of beta-carotene in tobacco to produce beta-ionone are constructed. The biosynthesis of the tobacco aroma component beta-ionone is realized, and the method has high efficiency.

A liquid tobacco extract is provided, including: a non-aqueous solvent; at least 0.2 percent by weight of nicotine based on a weight of the liquid tobacco extract; and a ratio by weight of (β-ionone+β-damascenone) to (phenol) of greater than 0.25.

The invention discloses a method for detecting sweet-scented osmanthus aroma components in sweet-scented osmanthus wine, which comprises the following steps: diluting a wine sample, adding salt until the wine sample is saturated, and then carrying out liquid-liquid micro-extraction by using a mixed solvent of absolute ether and ethyl acetate in a specific ratio, the volume fraction of the ethyl acetate being 3-5%; after oscillation, ultrasonic-assisted extraction and centrifugal separation, an organic phase is taken for dehydration, a gas chromatograph-mass spectrometer is used for analysis, and an internal standard method is adopted for quantification. By optimizing the polarity of the solvent, various aroma components (such as leaf alcohol, linalool oxide, linalool, ionone, tea aroma spirane and the like) from strong polarity to weak polarity can be effectively covered, and balanced and efficient extraction under a single extraction step is realized. The method is easy and convenient to operate, high in recovery rate, good in reproducibility and suitable for quality evaluation and production process control of the osmanthus fragrans wine.

The invention discloses a plant-derived repellent and its application in repelling tropical bed bugs and fire ants. The invention screens out three active ingredients of Chinese herbal essential oils, namely, α-ionone, citronellyl acetate and lilyal, wherein α-ionone and lilyal are derived from sandalwood, and citronellyl acetate is derived from citronella, and it is confirmed that the three substances have significant repellent effects on tropical bed bugs and fire ants. α-ionone, citronellyl acetate and lilyal are pure natural active substances of plant origin, have no toxic side effects on human body and environment, and are particularly suitable for preventing and controlling tropical bed bugs and fire ants in places where people live or often move.

The invention provides an alpha ionone composition and a preparation method thereof. The alpha ionone composition comprises ionone, an isomer and 0.0001-0.01 wt% of an impurity A, wherein the structure of the impurity A is as shown in a formula 1: # imgabs0 #. The generation of the key impurity A is controlled by limiting the content of the preisomer impurity of the pseudoionone in the reaction raw materials, the method is simple to operate, a typical violet fragrance product can be obtained, the fragrance quality of the product is superior to that of a competitive product in the market, and the product is applied to high-end cosmetics and daily cosmetics.

The invention discloses a regulator for improving grape fruit fragrance and a preparation method thereof, and belongs to the technical field of grape cultivation, each liter of regulator comprises 50 mg-120 mg of chitosan oligosaccharide, 20 mg-50 mg of sodium selenite, 10 mg-20 mg of jasmonic acid methyl ester, 100 mg-250 mg of green tea polyphenol, 10 mg-20 mg of trehalose, 1 mL-5 mL of thymol nanoemulsion, 10 mg-20 mg of beta-cyclodextrin and 0.8 g-1. 0 g of ethyl alcohol, and water is used as a solvent. The content of strawberry ketone, ester and norisoprene compounds in the fruits to which the regulator is applied is obviously increased, nerol and beta-ionone are detected, and the variety of terpene aroma substances is enriched; after the fruit is stored in a high-temperature and high-humidity environment for 3 days, the aroma component change of the fruit to which the regulator is applied is relatively insignificant, and the aroma of the fruit is more stable than that of a reference substance and a blank reference substance.

The application discloses a method for improving the separation yield of pseudo-isomethyl ionone, which comprises the following steps: condensation reaction of butanone and citral in a solvent under the action of an alkaline catalyst; after neutralization of the reaction solution, part of the solvent and unreacted raw materials are removed through a first desolventizing tower; the material in the first desolventizing tower is mixed with water and then enters a second desolventizing tower; and the liquid in the second desolventizing tower enters an oil-water separation tank to obtain a crude pseudo-isomethyl ionone product and waste brine. The method adopts double-tower continuous separation to remove the reaction solvent of pseudo-isomethyl ionone, and water is added between the two towers, so that water washing and salt removal are realized during the separation, and the separation yield of the crude product is greater than 99%. The method is simple in operation and is an economical separation method of pseudo-isomethyl ionone.

The invention provides a method for high-selectivity synthesis of alpha-ionone, and the high-selectivity alpha-ionone can be stably obtained by controlling the ratio of cis-isomer to trans-isomer of pseudoionone to (E) / (Z) to be more than or equal to 1 and less than or equal to 4. The method is suitable for stable industrial production of alpha-ionone.

The invention provides a laver seasoning base material as well as a preparation method and application thereof. In the preparation method, pectinase, cellulase and amylase are used for extraction in enzyme-assisted extraction, and protease and flavourzyme are used for conversion in flavor conversion. The laver seasoning base material prepared by the preparation method contains GGGTIGHPD, IGY, SFY, FYA, IAGF and IGGIF which are formed by enzymatic conversion and have small molecule peptides capable of enhancing flavor and improving oxidation resistance; the laver beverage is rich in laver oligosaccharide, algal peptide, flavor amino acid and taurine; the invention relates to the technical field of chemical engineering, in particular to a limonene and thiophene-3-ketone compound which is rich in limonene, thiophene-3-ketone, ethyl acetate, 2-hexenol, butyl acetate, dihydro-2-methyl-3-(2H)-furanone, 2, 6-dimethylpyrazine, 2-pentylfuran, 2-hexenal, benzaldehyde, 2, 3-octanedione and beta-ionone. According to the preparation method disclosed by the invention, full-effect utilization of nutrition and flavor components of the laver can be realized, and the prepared laver seasoning base material is delicious in taste, rich in nutrition, strong in flavor and clear in solution and can be quickly dissolved in water; the product can be used as various instant soup bases, cooking seasonings and even making base materials of various foods, and has good market application prospects.

An agent containing at least one aroma compound selected from the group consisting of E-beta-damascone, S-(2-methyl-3-furyl)ethanethioate, beta-caryophyllene oxide, beta-ionone, 2,5-dihydroxy-1,4-dithiane, methyl anthranilate, S-furfuryl thioformate, 1-isothiocyanate-3-(methylthio)propane, nootkatone, 1,4-dioxacycloheptadecane-5,17-dione, sclareol, and sclareolide is effective for masking an unpleasant smell and for making oral products in which an unpleasant smell is masked.

To suppress fading of food and drink colored with an anthocyanin-based pigment.SOLUTION: An effective amount of at least one kind selected from the group consisting of furaneol, vanillin, maltol, ethyl butyrate, hexyl acetate, trans-2-hexenal, isoamyl acetate, methyl hexanoate, ethyl 2-methyl butyrate, ethyl 3-hydroxyhexanoate, alpha-ionone and damascenone is added to food and drink colored with an anthocyanin-based pigment. In addition, a pigment preparation and a food and drink composition containing the same components are provided.SELECTED DRAWING: None