41 results about "Dust mites" patented technology

The invention relates to the technical field of allergy detection, in particular to an inhalation allergen specific IgE antibody detection kit and application thereof. The kit is a colloidal gold detection kit. The detection objects are pollen, dust mites and animal scurf; the kit comprises a colloidal gold labeled mouse anti-human IgE antibody arranged on a test strip combination pad; the pollen antigens (Art v1, Amb a1 and Bet v2), the dust mite antigens (Der f1 and Der p1) and the animal scurf antigen (Can f1) are arranged on a nitrocellulose membrane of the test strip. The kit provided by the invention can be used for rapidly identifying the allergen of the inhalation allergy symptom and classifying the severity of the allergy symptom, and the identification result has relatively high accuracy.

The invention relates to the field of antibodies, in particular to an anti-Der p 1 antibody and application thereof. The invention provides an anti-Der p 1 antibody, the anti-Der p 1 antibody comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region comprises an amino acid sequence of a complementarity determining region, and the amino acid sequence of the complementarity determining region comprises CDR1 as shown in SEQ ID No.1, CDR2 as shown in SEQ ID No.2 and CDR3 as shown in SEQ ID No.3; or the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No.8. The anti-Der p1 antibody provided by the invention is a monoclonal antibody with high affinity and high sensitivity, after the monoclonal antibody is subjected to humanized IgE transformation, the monoclonal antibody is applied to a Dermatophagoides pteronyssinus allergen component Derp1 specificity IgE magnetic particle chemiluminescence quantitative detection reagent, and a standard curve independently calibrated by the Dermatophagoides pteronyssinus allergen component Derp1 can be obtained; real, accurate and repeatable absolute quantification is realized, the Dermatophagoides pteronyssinus allergen component Derp 1 specificity IgE with the concentration of 0.1 IU / mL can be detected, and the method has clinical diagnosis significance in allergic reaction identification.

This invention discloses a method, system, equipment, and medium for real-time detection of the overall performance of a mite remover, specifically relating to the field of performance testing technology. The method includes sealing and connecting the mite remover under test with a test sample chamber containing a built-in standard dust mite test sample block; continuously acquiring instantaneous input power and instantaneous wind speed through a power acquisition unit and a hot-wire anemometer, and calculating the instantaneous intake flow rate based on the cross-sectional area of ​​the duct; continuously acquiring a sequence of microscopic images of the upper surface of the fiber fabric layer within a set detection period; extracting individual dust mite displacement events using the inter-frame difference method, counting the total number of events, and calculating the average displacement velocity value; and using the average intake power, the total number of individual dust mite displacement events, and the average displacement velocity value as input variables, generating a comprehensive evaluation value of the overall machine's performance through a performance judgment model. This invention integrates power, flow rate, and the dynamic response parameters of live dust mites, achieving real-time and accurate quantitative evaluation of the overall performance of the mite remover.

The invention discloses a construction method and application of a machin allergic rhinitis animal model, and belongs to the field of disease animal model construction. According to the method, house dust mites are firstly used for sensitization, then 10% hydrogen peroxide and house dust mites are combined for excitation modeling, the 10% hydrogen peroxide destroys nasal mucosa and accelerates occurrence of allergic rhinitis, and the monkeys with allergic rhinitis are obtained after 13 periods of modeling. According to the method, the animal model which is highly similar to human allergic rhinitis in behavior, inflammatory symptom and phenotype is successfully constructed. The model provides guarantee for observing nasal symptoms and rhinitis behaviors of allergic rhinitis, inducing an organism to generate allergy-related immune response, establishing a sensitization basis, simulating a pathogenesis process, researching pathogenesis of allergic rhinitis, screening allergic rhinitis drugs and the like.

The application relates to a dust removal structure and a cleaning device, which comprises a shell and a brush head assembly. The shell has a receiving space and an opening communicating with the receiving space. The brush head assembly is arranged in the receiving space and rotationally connected with the shell. The brush head assembly can rotate around the axial direction of the receiving space, and at least part of the brush head assembly extends out of the receiving space through the opening and is arranged to intersect the plane of the opening in the axial direction. The brush head assembly has a guide channel extending in the axial direction of the receiving space. The shell also has a dust suction port. The dust suction port and the opening are located at the two ends of the axial direction of the receiving space. The guide channel is configured to guide the medium on the side of the opening to the dust suction port when the brush head assembly rotates. In this way, the brush head assembly pushes the medium, such as dust and dust mites, on the surface to be cleaned to the side of the dust suction port far away from the opening, so that dust suction can be facilitated, and the dust removal efficiency is improved.

The invention discloses a construction method of an animal model for combined induction and exacerbation of asthma. The method comprises the following steps: injecting ovalbumin or dermatophagoides pteronyssinus extract into an intraperitoneal cavity for sensitization; aerosol inhalation of allergen is carried out for airway re-exposure; in the aggravating stage, pathogenic microorganisms such as pseudomonas aeruginosa, aspergillus fumigatus or respiratory syncytial virus are given and exposed to 500 mu g / m PM2.5 aerosol; lung tissue and bronchoalveolar lavage fluid are then detected. The established model shows that the airway resistance is obviously enhanced and persistent, the mucous thrombus formation rate is greater than 50%, the smooth muscle is obviously thickened, and the model is accompanied by eosinophilic granulocyte and neutrophil infiltration and high expression of various inflammatory factors. The model is good in stability and high in repeatability, can truly simulate clinical asthma acute exacerbation and steroid insensitive characteristics, and provides a platform for asthma mechanism research and new drug screening.

The invention relates to the field of allergen detection, in particular to an anti-Der p 2 antibody and application thereof. The invention provides an anti-Der p 2 antibody. A heavy chain variable region of the anti-Der p 2 antibody comprises an amino acid sequence of a complementarity determining region: CDR1 as shown in SEQ ID No.1, CDR2 as shown in SEQ ID No.2, and CDR3 as shown in SEQ ID No.3; or the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No.8. According to the protein, a New Zealand rabbit is immunized by utilizing Der p 2 protein of a main allergen of Dermatophagoides pteronyssinus, and a group of monoclonal antibodies with high affinity and high sensitivity to Der p 2 are obtained through screening. The antibody is further subjected to humanized IgE modification and is applied to a magnetic particle chemiluminescence quantitative detection reagent of Der p 2 specific IgE. According to the reagent, a standard curve for Der p 2 independent calibration can be established, so that accurate and repeatable absolute quantitative analysis is realized, and the detection sensitivity can reach 0.1 IU / mL. The detection method formed on the basis of the anti-Der p 2 antibody provided by the invention has important value for clinical diagnosis of anaphylactic reaction.

To provide a decorative sheet having an allergen reducing performance exhibiting an allergen reducing effect to mite and cedar, while maintaining surface performance of a surface protective layer.SOLUTION: A decorative sheet comprises a crosslink-cured resin layer on the outermost surface. (1) The crosslink-cured resin layer contains a cured product of a crosslink-cured resin and an allergen-reducing agent. (2) The allergen-reducing agent is an allergen-reducing agent having an allergen-reducing performance on mites and cedar, contains a carboxylic acid derivative and a styrene polymer derivative, and contains all of triethylamine, N,N-dimethylallylamine, dimethylpyrrole, tetramethyl-1,3-propanediamine, and N,N,2,2-tetramethyl-1,3-propanediamine as components of at least the carboxylic acid derivative. (3) The crosslink-cured resin layer further contains two different types of fine particles.SELECTED DRAWING: None

The application relates to the technical field of allergy detection, in particular to an inhaled allergen specific IgE antibody detection kit and application thereof. The kit is a colloidal gold detection kit; the detection object is pollen, dust mites and animal dander; the kit comprises colloidal gold labeled mouse anti-human lgE antibodies arranged on a test strip combination pad; and pollen antigens (Art v1, Amb a1, Bet v2), dust mite antigens (Der f1, Der p1) and animal dander antigens (Can f1) arranged on a test strip nitrocellulose membrane. The kit provided by the application can quickly identify the allergen of inhaled allergic symptoms, classify the severity of allergic symptoms, and the identification result has a high accuracy.

The invention relates to a method for obtaining a probiotic product for neutralizing allergens, released by house dust mites, product composition and method of use, which is from the technical field of providing a healthy microclimate based on a probiotic product. The product concerns the maintenance of household stuff in immediate and direct use by humans, such as shoes, blankets, upholstered furniture, clothes, carpets and mats, the air in domestic and office premises, as well as in bathrooms, toilets and vehicles. The product composition consists of a spore concentrate, containing minimum of 1x109 CFU / ml fermentation bacteria class 1 (one), ethanol, hypoallergenic perfume mixture, preservative and water, softened, processed by reverse osmosis and cavitation, ultra-filtered, and microbiologically clean in terms of bacteria and viruses.

The utility model discloses a filtering device with multiple filtering layers for an FFU fan, and relates to the technical field of filtering devices. The fan comprises a fan body, an air inlet cavity is fixedly connected to the top face of the fan body, a filter cavity is formed in the fan body, a first filter element and a second filter element are arranged in the filter cavity, plugging assemblies are arranged in the filter cavity and located on the upper sides and the lower sides of the first filter element and the second filter element, and each plugging assembly comprises a sealing disc. Wherein one sealing disc corresponds to and is matched with a cavity opening of the air inlet cavity, the other sealing disc corresponds to and is matched with the air outlet end of the fan body, and an ozone generator is arranged on the upper portion of the inner side wall of the filtering cavity. According to the air purifier, tiny particles such as bacteria, viruses and dust mites in air can be filtered out, the bacteria on the first filter element and the second filter element can be killed, and the cleanliness of the filtered air is prevented from being affected.

The invention discloses portable equipment for improving and preventing rhinitis with small-particle-size negative ions and a use method, and relates to the technical field of rhinitis prevention, the portable equipment comprises a product front shell, the equipment can release small-particle-size and high-activity negative ions by selecting an ecological negative ion generation chip technology, and the negative ions enter the nasal cavity through breathing and act on blood capillaries and nerve endings, so that the rhinitis is improved and prevented. The traditional Chinese medicine composition can regulate vasomotor, improve nasal cavity blood circulation, relieve mucous membrane hyperemia and swelling and relieve nasal obstruction, has the mucous membrane repairing function, promotes nasal mucous membrane epithelial cell regeneration, recovers ciliary movement and secretion functions, enhances the self-cleaning capacity of the nasal cavity, and can regulate whole body immunity, reduce the sensitivity of nerves to mechanical stimulation and reduce anaphylactic reactions such as sneezing. The medicine can activate the reticuloendothelial system, enhance the disease resistance of the organism, reduce rhinitis recurrence and remove environmental allergens, small-particle-size negative ions can adsorb and settle allergens such as dust mites and pollen in air, nasal cavity stimulation is reduced from the source, and double barriers for treatment and prevention are formed.

The invention relates to the technical field of textile, and discloses a high-density professional anti-mite dustproof pure cotton tribute silk fabric which comprises a surface layer and an inner layer, and the surface layer and the inner layer are integrally formed through a weaving process; the surface layer is formed by weaving 80S pure cotton yarns, the warp-wise density of the surface layer is 235 pieces per inch, the weft-wise density of the surface layer is 200 pieces per inch, and the aperture range of the surface layer is 0.14 mu m-0. 5 mu m; the inner layer is formed by weaving 60S pure cotton yarns, the warp-wise density of the inner layer is 205 pieces per inch, the weft-wise density of the inner layer is 180 pieces per inch, and the aperture range of the inner layer is 0.5-1 [mu] m. Through the innovative design of the double-layer gradient aperture structure, accurate partition and synergistic effect of fabric functions are achieved, the compact structure of the outer layer effectively blocks dust mites and fine particles, and the relative loose structure of the inner layer remarkably improves the breathability and comfort level; the long-standing core contradiction that the protection performance and the breathable comfort of the high-density anti-mite fabric are difficult to consider at the same time is successfully solved.

The application relates to a brush head assembly, a dust removal structure and cleaning equipment. The brush head assembly comprises a main body, a guide part and a dust lifting part. The guide part is arranged on the main body, and the guide part and the main body define a guide channel extending spirally along the axial direction of the main body. The dust lifting part is arranged on the main body. The brush head assembly has a working state. In the working state, at least part of the dust lifting part is in contact with a surface to be cleaned and forms a dust lifting area. The dust lifting area is in communication with the entrance of the guide channel, and the medium in the dust lifting area can flow along the extension direction of the guide channel. The medium is guided along the axial direction of the main body, so that the dust, dust mites and other media on the surface to be cleaned can be pushed to a far position for dust collection, thereby improving the dust removal efficiency.

The present application relates to a kind of acarid removing instrument, acarid removing instrument includes shell and is arranged in the steam component of shell, steam outlet piece, roll brush component and dust suction component;Steam component has the steam outlet of can output steam;Steam outlet piece is configured with steam passage in, steam passage is communicated with the steam outlet of steam component, and steam passage is opened with the exhaust hole of communication with outside on, the steam flowing out from steam outlet can be discharged from exhaust hole;Exhaust hole is opened towards the surface to be cleaned;Roll brush component includes roll brush, shell is configured with first accommodating cavity, roll brush is rotatably arranged in first accommodating cavity, roll brush can be rolled contact with the surface to be cleaned;Dust suction component has the dust suction port of communication with first accommodating cavity, dust suction port is used to be rolled contact with the surface to be cleaned and the dust mite brought up by roll brush is inhaled.The acarid removing effect of the present application is good.

1. The name of the design product: dust mite suction cleaner (base station). 2. The use of the design product: for dust removal and mite removal. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The utility model provides a dust cup structure and acarus killing instrument, wherein the dust cup structure comprises: a main body, the main body has an opening, the opening is provided with a cover body, the main body is internally and detachably provided with a separator, the separator divides the inner cavity of the main body into a first chamber and a second chamber, the second chamber is internally provided with a separating assembly, and the separating assembly is provided with an opening. And the first chamber is communicated with the second chamber. According to the dust cup, the technical problem that in the prior art, the dust cup of the acarus killing instrument needs to be cleaned through an external tool, and cleaning operation is troublesome can be solved.

The invention provides bacillus amyloliquefaciens capable of degrading allergens and application of the bacillus amyloliquefaciens. The bacillus amyloliquefaciens is preserved in the China General Microbiological Culture Collection Center (CGMCC) on October 9, 2025, the preservation number is CGMCC No.36139, and the bacillus amyloliquefaciens can be used for degrading dust mite allergens, cat allergens and dog allergens.

The invention relates to the technical field of filters, and particularly discloses a filter material for filtering cooking fume and a preparation method of the filter material. The filter material comprises: an aggregate fiber layer as a base material; the activated carbon layer comprises modified activated carbon mixture particles, and the modified activated carbon mixture particles are prepared by mixing activated carbon particles and a phosphoric acid catalyst and then drying; the biological functional layer comprises a non-woven fabric, and the non-woven fabric contains polyphenol substance particles; the aggregate fiber layer, the activated carbon layer and the biological functional layer are sequentially laminated and bonded together from top to bottom to form the filter material. According to the scheme, the filter material is formed by compounding the aggregate fiber layer, the activated carbon layer and the biological functional layer, and has a good adsorption and removal effect on fishy smell and other peculiar smells, pollen and dust mites.

The invention provides modified hydroxyapatite for adsorbing dermatophagoides pteronyssinus allergens as well as a synthesis method and application of the modified hydroxyapatite. Dermatophagoides pteronyssinus allergen in the air is mainly suspended in the form of protein, and when exposed in the environment, a human body passively inhales or contacts with the dermatophagoides pteronyssinus allergen to induce allergic rhinitis, asthma and other diseases. The modified hydroxyapatite has a unique porous crystal structure, the content of the main component Ca10 (PO4) 6 (OH) 2 is larger than or equal to 99 wt%, the surface of the modified hydroxyapatite is rich in hydroxyl (-OH), functional groups such as amino (-NH2) and carboxyl (-COOH) can be introduced through targeted modification, and the modified hydroxyapatite is in a sheet-shaped or rod-shaped crystal shape. The dust mite allergens in air are removed through the synergistic effect of physical adsorption and chemical adsorption, and the removal rate of the dust mite allergens Der p 1 and Der f 1 is increased under the room temperature condition. The invention further discloses a synthesis method of the modified hydroxyapatite and application of the modified hydroxyapatite in the field of air purification, and an efficient solution is provided for indoor allergen prevention and control.

The invention relates to the field of immunology and cell therapy, in particular to application of rTh1 cells in preparation of drugs for preventing or treating allergic diseases or inflammatory diseases, the phenotypes of the rTh1 cells are CD3 (+), CD4 (+), CD25 (-), Foxp3 (-), CXCR5 (-), CCR6 (-), CXCR3 (+), CD73 (+) and cMaf (+), and FR4, PD1 and ICOS are highly expressed. The novel T cell subset rTh1 cell has an immunoregulation function, and has an important value in prevention and treatment of allergic diseases such as food allergy and dust mite allergy, atopic dermatitis, allergic asthma, chronic obstructive pulmonary disease and other Th2-type inflammation related skin diseases such as pemphigus bullosa and urticaria.

The application discloses a preparation method of an inhaled mixed allergen storage solution. The storage solution is used for storing mixed allergens composed of house dust mites, dust mites, cat dander, horse dander, dog hair, timothy grass, cladosporium, common birch, olive, artemisia and medicinal wall grass, and the storage solution comprises the following components in the form of mass fractions: (1) 5mM-50mM PBS buffer solution, pH 7.0-8.0, 0.5%-5% salt, 5%-30% human serum albumin, 0.05%-0.2% preservative, and 0.1%-1% protein stabilizer. The preparation method and application of the inhaled mixed allergen storage solution effectively solve the cross reaction between various proteins, and realize more sensitive detection of common inhaled allergens in samples.

The invention discloses application of Der p sIgE and / or Der f sIgE in nasal secretion in preparation of a kit or a detection platform for diagnosing local allergic rhinitis, and relates to the technical field of in vitro diagnosis and immunoassay. The invention relates to application of Der p sIgE and / or Der f sIgE in nasal secretion in preparation of a kit or a detection platform for diagnosing local allergic rhinitis. The invention proposes and verifies that the dust mite sIgE in the nasal secretion can be used as an effective biomarker for diagnosing LAR for the first time, and solves the technical problem of difficulty in typing serum sIgE negative rhinitis patients clinically. Detection can be completed only by collecting nasal secretions, operation is easy and convenient, safety is high, and clinical popularization is easy. According to the method, diagnosis is objective and accurate, a diagnosis critical value is scientifically established through research on healthy people, a quantitative objective diagnosis standard is provided, it is verified through the cell factor level that the diagnosis result is highly consistent with the intrinsic biological mechanism of allergic inflammation, and the accuracy is high.

The present invention relates to a pharmaceutical composition for preventing, treating or ameliorating respiratory diseases, comprising an Agrimonia pilosa extract as an active ingredient. The pharmaceutical composition can alleviate inflammatory responses occurring in lung tissues and reduce allergic responses, and thus can be effectively used for preventing, ameliorating or treating various respiratory diseases, particularly lung diseases caused by house dust mites.

The application discloses a kind of construction methods of combined induction aggravation asthma animal model.The method includes: abdominal cavity injection egg white protein or house dust mite extract is sensitized;Allergen is inhaled by atomization and is exposed to airway;In aggravation stage, give pseudomonas aeruginosa, lung fumagine or respiratory syncytial virus and expose to 500 μg / m 3 PM2.5 aerosol;Subsequently, lung tissue and bronchoalveolar lavage fluid are detected.The model shows that airway resistance is significantly enhanced and continues, the rate of mucus plug formation is greater than 50%, smooth muscle is significantly thickened, accompanied by eosinophil and neutrophil infiltration and high expression of various inflammatory factors.The model of the application has good stability, strong repeatability, can truly simulate clinical asthma acute exacerbation and steroid-insensitive characteristics, and provides a platform for asthma mechanism research and new drug screening.

The invention relates to an IgG4 antibody specifically combined with dermatophagoides pteronyssinus extract and a preparation method thereof. The screening method comprises the following steps: 1) stimulating a lymph node organ with an antigen; 2) analyzing the stimulated lymph node organoid by combining single cell sequencing and an immune repertoire, and screening a potential IgG4 sequence; (3) preparing the potential IgG4 sequence into potential antibody protein in an in-vitro expression manner; and 4) respectively carrying out ELISA detection and biological layer interference technology verification on the potential antibody protein, wherein the antibody which can be specifically combined with the antigen is the IgG4 antibody. An antigen binding fragment of the obtained IgG4 antibody is formed by connecting a heavy chain fragment and a light chain through a disulfide bond, the nucleotide sequence of the heavy chain fragment is shown as any one of SEQ ID No: 1 to SEQ ID No: 3, and the nucleotide sequence of the light chain is shown as any one of SEQ ID No: 4 to SEQ ID No: 6.

The present invention relates to a method for producing an aging model skin cell line by using house dust mites and, more specifically, to a method for producing an aging model skin cell line, and an aging model skin cell line produced using same, the method comprises the steps of: culturing isolated skin cells in a medium comprising fetal bovine serum; and adding a purified house dust mite extract at a concentration of 10 to 300 μg / ml to the cultured skin cells so as to induce aging of the cultured skin cells. The present invention provides the aging model skin cell line, which can be used as an alternative to animal experiments and has the effect of not causing a decrease in copy number even upon subculture.

The present application relates to the technical field of air purification, and discloses a mite-removing air purifier and a control method, a control device and a storage medium thereof. The mite-removing air purifier comprises: a purification body having a purification air inlet, an air outlet and a purification air duct connecting the purification air inlet and the air outlet; and a mite-removing device arranged in the air purification body, wherein the mite-removing device has a mite-removing air duct, one end of the mite-removing air duct is formed into a mite-removing inlet matched with an article to be mite-removed, the other end of the mite-removing air duct is communicated with the air outlet, and the mite-removing device has an unfolded state and a contracted state. The mite-removing air purifier has a purification air mode and a mite-removing mode. When the mite-removing air purifier is in the purification air mode, the mite-removing device is in the contracted state. When the mite-removing air purifier is in the mite-removing mode, the mite-removing device is in the unfolded state, and the mite-removing device is in contact with the article to be mite-removed after being unfolded, so as to remove mites from the article to be mite-removed. The mite-removing air purifier has the functions of purification and mite removal, and has a high utilization rate.

The invention relates to the technical field of rhinitis fumigation treatment, in particular to an allergic rhinitis fumigation device which comprises a movable fumigation machine and a medicine steam conveying guide pipe connected to the fumigation machine and further comprises a double-helix nasal cavity fumigation module connected to the medicine steam conveying guide pipe. The double-helix nasal cavity fumigation module is used for removing nasal mucus secreted in the nasal cavity of a patient and allergens attached to the nasal mucus and conducting fumigation treatment, a fixing belt is arranged on the double-helix nasal cavity fumigation module, and the problems that in the prior art, devices only have the one-way medicine steam conveying capacity, mucus, nasal mucus and the like on the nasal mucosa surface and cilia cannot be removed, and fumigation treatment is affected are solved. Due to the fact that allergens such as dust mites and pollen particles adhere to snot and stay in the nasal cavity of a patient, symptoms cannot be quickly and effectively relieved, nasal cavity secretions can physically block medicine steam, the fumigation treatment effect is remarkably reduced, meanwhile, the air tightness and comfort are poor, the nasal cavity of the patient is prone to being damaged, and the problem that the nasal cavity of the patient is prone to being damaged is solved. And the use safety cannot be effectively guaranteed.