The invention is applicable to the technical field of clinical examination and auxiliary diagnosis, and provides an extreme behavior risk auxiliary diagnosis biomarker combination, a kit and a detection method, the biomarker combination is composed of IL-4, IL-12 and
IFN gamma, and the extreme behavior risk can be objectively identified by detecting the expression level of the combination in
peripheral blood of a subject. The matched kit comprises specific antibodies aiming at the three markers, a standard substance, a
buffer solution and other reagents, and according to the standardized detection method, through
peripheral blood sample treatment, incubation,
fluorescent staining, flow detection and result analysis, precise evaluation is achieved in combination with a clear judgment threshold value. According to the method, the defect that suicide
risk assessment depends on a subjective scale and doctor experience in the prior art is overcome, the operation is convenient, the result is objective, the combined detection AUC of the suicide
risk assessment, the subjective scale and the doctor experience can reach 0.726, a reliable extreme behavior risk auxiliary diagnosis means is provided for clinic, and the method is particularly suitable for
risk identification of depression patients and has important clinical application value and conversion prospect.