51results about How to "Reduce mortality" patented technology

The invention discloses a lactobacillus brevis with broad-spectrum bacteriostatic activity and a separation method and application thereof, and belongs to the technical field of microorganisms, in particular to a lactobacillus brevis AW04 with broad-spectrum bacteriostatic activity, which is characterized in that the lactobacillus brevis AW04 is preserved in the China Center for Type Culture Collection, the lactobacillus brevis AW04 is preserved in the China Center for Type Culture Collection, the lactobacillus brevis AW04 is preserved in the China Center for Type Culture Collection, and the lactobacillus brevis AW04 is preserved in the China Center for Type Culture Collection. The address is Wuhan University Campus, Wuchang District, Wuhan City, Hubei Province; the postcode is 430072; the preservation number of the strain is CCTCC M 20241970; the preservation date is September 13, 2024. The strain provides a novel, safe and efficient strain resource for developing microecological preparations, feed additives or veterinary drugs for preventing and treating bacterial diarrhea of animals such as calves and the like, and has important value for reducing the use of antibiotics in the breeding industry and coping with bacterial drug resistance crisis. The strain can be produced on a large scale by using the existing fermentation technology and is convenient to apply.

This invention provides a method for the artificial breeding of bullfrogs, including bullfrog selection, pairing, breeding, artificial spawning induction, artificial insemination, fertilized egg hatching, and tadpole rearing. This invention uses precise control of water temperature and quality in the breeding water to stimulate the gonadal development of the bullfrogs. It utilizes artificial spawning induction, insemination, fertilized egg hatching, and seedling rearing techniques to solve the problems of existing bullfrog germplasm degradation and difficulties in artificial breeding. By adding *Lactobacillus rhamnosus* to the feed, the mortality rate of bullfrogs is effectively reduced, the hatching rate of fertilized eggs is high, and the seedling growth rate is fast. This allows for a stable and continuous supply of high-quality bullfrog stock, meeting the effective demand for bullfrog seedlings throughout the year, and providing a technical foundation for the artificial breeding of bullfrogs.

This utility model relates to the field of aquaculture technology, specifically a buffer-type salmon and trout transfer device, including a conveying chamber with a fish storage box fixedly connected inside. This utility model features a first transport trough, a second transport trough, a rotating shaft, and a turntable. When the operator pulls the second transport trough outward, a slider inside the trough moves outward, extending the second transport trough so that its length contacts the water surface, reducing salmon and trout mortality due to height limitations. Pressing down on the second transport trough causes the first transport trough to rotate downward, which in turn causes the rotating shaft to rotate, allowing the second transport trough to contact the water surface. Pulling up the lifting block raises the gate, allowing the salmon and trout to flow from the fish storage box into the first transport trough. The cushioning effect of the rubber pad reduces salmon and trout mortality and improves the device's efficiency.

The invention discloses a Ruditapes philippinarum miR-263b inhibitor and application thereof, and finds that Ruditapes philippinarum miR-263b can regulate and control innate immune response of a host by targeting RpSC2, negatively regulates activation of an immune-related signal channel in a vibrio infection process, and further, on the basis of a molecular regulation and control mechanism, the Ruditapes philippinarum miR-263b inhibitor can be used for inhibiting the infection of vibrio. The invention discloses a specific inhibitor which is completely complementary with a miR-263b mature body sequence. Experimental data show that after Ruditapes philippinarum is injected with the miR-263b inhibitor, the expression level of miR-263b in vivo is reduced, and the inhibition effect of miR-263b on RpSC2 is effectively relieved, so that the expression level of a target gene RpSC2 is up-regulated, an NF-kappa B immune signal channel is activated, the immune defense capability of the Ruditapes philippinarum on vibrio anguillarum is enhanced, the death rate after infection is remarkably reduced, and the Ruditapes philippinarum is prevented from being infected. The in-vivo bacterium loading amount is obviously reduced, and the pathological injury of hepatopancreas tissue is obviously relieved.

The application discloses a potential molecular marker for assisting in diagnosing cancer. The application provides application of a methylation CD44 gene as a marker in preparation of a product; the product is used for at least one of the following: assisting in diagnosing cancer or predicting a cancer morbidity risk; assisting in distinguishing a benign nodule and cancer; assisting in distinguishing different subtypes of cancer; assisting in distinguishing different stages of cancer; assisting in distinguishing different cancers; determining whether a to-be-tested substance hinders or promotes occurrence of cancer; and the cancer can be lung cancer or breast cancer. The application finds a low methylation phenomenon of the CD44 gene in blood of lung cancer and breast cancer patients, and has important scientific significance and clinical application value for improving early diagnosis and treatment effect of lung cancer and breast cancer and reducing mortality.

The present application relates to the technical field of microorganism, and especially relates to Enterococcus hirae C01, probiotic agent and application thereof.The present application provides the Enterococcus hirae C01, and the preservation number is CGMCC No.33994.The strain has broad-spectrum bacteriostatic activity, can inhibit Salmonella enteritidis, Salmonella pullorum and Salmonella typhimurium, has no hemolytic activity, has good stress resistance, can survive in gastrointestinal environment, and has high biological safety, and can be used for preventing and controlling mixed Salmonella infection in clinic.

The application provides a mucosal lactobacillus with anti-feline pestivirus pathogenic effect and application thereof, and relates to the technical field of probiotics, veterinary medicine and pet health care. The mucosal lactobacillus with anti-feline pestivirus pathogenic effect is named mucosal lactobacillus WH-3221, and is preserved in the China Center for Type Culture Collection with a preservation number of CCTCC NO: M 2026683. The mucosal lactobacillus WH-3221 can realize protection of intestinal barrier by regulating intestinal metabolic product spectrum, and has an anti-feline pestivirus effect, thereby significantly reducing the mortality of cats infected with feline pestivirus and relieving clinical symptoms. The mucosal lactobacillus WH-3221 or a probiotic preparation containing the mucosal lactobacillus WH-3221 can be used for preparing products for preventing and / or treating diseases or symptoms caused by feline pestivirus infection, and for preparing products for regulating the intestinal metabolic product spectrum of cats, and has a wide application prospect.

The invention belongs to the technical field of biological medicines, and discloses an artificially synthesized antibacterial peptide, a derivative thereof and an application of the artificially synthesized antibacterial peptide in resisting escherichia coli. The antibacterial peptide provided by the invention has a strong inhibition effect on escherichia coli and comprises multiple drug-resistant strains. Experiments show that the antibacterial peptide has high in-vitro antibacterial activity, excellent thermal stability, acid-base stability and no hemolytic activity, has no obvious toxicity to mammalian cells, and shows a remarkable treatment effect in a chicken escherichia coli infection model. The invention provides a novel antibacterial preparation for coping with escherichia coli, especially infection of drug-resistant strains.

The present application relates to the technical field of aquaculture net cage control, and particularly relates to a net cage suspension depth fixing information sensing monitoring and adjusting system, which comprises the following steps: collecting multi-source sensor data to provide comprehensive data support for net cage state monitoring; generating a net cage real-time suspension state image through data fusion processing to provide a reliable basis for decision making; generating a multi-dimensional adjusting scheme through judging the suspension state to ensure that the net cage is quickly restored to the set state; executing adjusting instructions through multiple adjusting units to ensure the safe operation of the net cage; and establishing a historical strategy retrieval and optimization mechanism to optimize control parameters and improve the long-term operation efficiency of the system. The present application is used to solve the problems of traditional net cages, such as lagging in suspension state regulation and control under complex sea conditions and poor stability, and can provide a continuous and stable optimal growth water depth and posture for high-value aquaculture organisms sensitive to the environment, effectively improve the survival rate and breeding efficiency of good strains, and has significant industrial application value.

The present invention relates to high purity cangrelor, pharmaceutical formulations comprising high purity cangrelor as an active ingredient, methods for preparing such compounds and formulations, and methods for using the pharmaceutical formulations in the inhibition of platelet activation and aggregation.

This invention relates to the field of biomedical technology, and more particularly to the application of an isoflavone compound, specifically Corylifol A, in the preparation of drugs for the prevention and / or treatment of inflammatory diseases. When used to prepare drugs for the prevention and / or treatment of inflammatory diseases, this isoflavone compound can directly bind to the IκBα protein and specifically inhibit its phosphorylation, thereby blocking the phosphorylation and nuclear translocation of the NF-κB p65 subunit, ultimately inhibiting the overactivation of the NF-κB signaling pathway. Furthermore, the isoflavone compound can significantly reduce the mortality rate of lipopolysaccharide-induced septicemia in mice, inhibit the levels of inflammatory factors in serum and lung tissue, and effectively alleviate LPS-induced acute lung injury in mice. Simultaneously, when the isoflavone compound is controlled within an effective concentration range, it exhibits no significant cytotoxicity to mouse peritoneal macrophages and can significantly inhibit the production and release of lipopolysaccharide-induced inflammatory factors IL-1β, TNF-α, and IL-6.

The present invention relates to high purity cangrelor, pharmaceutical formulations comprising high purity cangrelor as an active ingredient, methods for preparing such compounds and formulations, and methods for using the pharmaceutical formulations in the inhibition of platelet activation and aggregation.

This invention relates to the field of medical immunology, and particularly to the application of CD39+ regulatory T cells as a biomarker in products for prognostic assessment of childhood sepsis. This invention is based on flow cytometry detection of CD39+ T cells in peripheral blood of patients. + Treg levels, and dynamically assess patient prognosis based on changes in their expression. CD39 + Treg expression levels were significantly negatively correlated with childhood sepsis scores (Phoenix and pSOFA scores) and negatively correlated with mortality rates in children with sepsis. When CD39... + When Treg expression is below 12.45%, it indicates a higher risk of death in the patient. The method of this invention can provide accurate prognostic assessment in the early screening of childhood sepsis, with high specificity and sensitivity. This method can also be used for dynamic monitoring of patients, helping doctors to adjust treatment plans in a timely manner and optimize personalized treatment, demonstrating significant practical application value.

This invention discloses the application of a Trichinella spiralis cysteine ​​protease inhibitor in acute myocardial infarction and subsequent ventricular remodeling, belonging to the field of medicinal chemistry. This invention provides a drug that can prevent, alleviate, and / or treat acute myocardial infarction and subsequent ventricular remodeling. The active ingredient of this drug is the Trichinella spiralis cysteine ​​protease inhibitor cystatin, abbreviated as rTs-Cys. rTs-Cys can effectively delay acute myocardial infarction and inflammatory response in mice, reduce the infarct area, improve cardiac function deterioration, and reverse organ pathological damage, providing a new approach for the clinical treatment of acute myocardial infarction and subsequent ventricular remodeling.

This specification provides a system and apparatus for constructing an animal model of spinal glioma, relating to the field of intelligent medicine. The system, executed by a robot, includes: a puncture point localization module configured to locate the highest point of the spine in its natural state as the puncture point; a spine exposure module configured to locate points at a first distance above and below the highest point of the spine along the spinal direction, forming a line segment; controlling the cutting of the dorsal skin of the animal sample along the line segment to expose the spine; a puncture needle insertion module configured to control the puncture needle to penetrate the puncture point perpendicular to the vertebral interface at a first speed until the puncture needle penetrates the spinal cord tissue; controlling the puncture needle to retract a third distance and pause for a first time; a cell suspension injection module; and a puncture needle withdrawal module configured to control the withdrawal of the puncture needle, thus obtaining the animal model. This application provides insights for clinical research on the molecular characteristics of spinal glioma and the identification of potential therapeutic targets.

The invention belongs to the technical field of biological medicines, and particularly relates to a method for promoting proliferation of immature bone marrow-derived macrophages and polarization of the immature bone marrow-derived macrophages to M1 through stress application culture and application of the method. The in-vitro stress application culture method of the macrophages from the immature bone marrow has the characteristics of high stability, high amplification efficiency, polarization controllability, high purity, flexibility in application, low production cost and the like; the problems that a traditional cell factor induction method is low in amplification efficiency, poor in polarization controllability, insufficient in purity and the like are solved, the purity of the obtained M1 type macrophage reaches 90% or above, stability is high, and cost is low. The method can be applied to preparation of macrophage drugs for immunoregulation, anti-fibrosis, metabolism regulation, anti-tumor CAR-M and the like, tissue regeneration biological materials, anti-inflammatory drugs, in-vitro diagnosis products and the like, dosage forms are diversified, and a standardized cell preparation scheme is provided for the related biological medicine field.

The present application relates to the technical field of disease risk prediction, and particularly relates to a ten-year risk prediction method, system and device for sudden cardiac death, the method comprising: obtaining 48-hour electrocardiogram data of an individual sample and a risk classification evaluation result based on a China-PAR risk assessment model; splitting and combining the electrocardiogram data to obtain a preset multi-dimensional waveform graph; constructing a convolutional neural network model, taking the waveform graph as input and the corresponding risk classification evaluation result as output to train the model and obtain a sudden cardiac death risk prediction model; inputting the processed waveform graph of 48-hour electrocardiogram data of a subject into the prediction model, and outputting the result as a ten-year sudden cardiac death risk prediction level result. The present application can create a non-linear, high-precision, and iterative sudden cardiac death risk deep learning model for the general population and doctors, improve the detection rate of sudden cardiac death, and reduce the recurrence rate and mortality.

The invention discloses an application of FKBP12B in improving the survival rate of silkworms and the quality of silk. According to the invention, it is found for the first time that after the FKBP12B DNA is given to silkworms, the survival rate, the cocoon shell weight, the cocoon shell rate and the silk quality of the silkworms can be improved, which indicates that FKBP12B has a potential application value of improving the silk quality.

This application relates to a method and apparatus for emergency resource matching and rescue decision-making based on injury situation. The method includes: acquiring real-time data from the disaster site; constructing a dynamic situational awareness model based on the real-time data and generating a multi-objective, multi-constraint rescue task model; solving the rescue task model using an intelligent matching algorithm and a hybrid multi-objective evolutionary algorithm to generate an initial casualty-resource matching scheme and a rescue route planning scheme; continuously monitoring situational indicators during the execution of the initial scheme, and performing a replanning operation to adjust resource matching and rescue routes when any indicator triggers a preset dynamic grading threshold. This method can significantly shorten the waiting time for critically injured patients, improve resource utilization, and reduce the expected mortality rate. It achieves efficient, robust, and adaptive rescue decision-making in large-scale emergency scenarios.

The utility model relates to agricultural waste treatment equipment technical field especially based on light and heat dual drive's black soldier fly larvae and insect sand separation system, include: frame, upper layer separation bin and upper layer separation bin. Upper layer separation bin sets up in the frame top, including the netted conveyer belt of horizontal operation, the flattening scraping thin device of feed end, the light and heat source above netted conveyer belt and the screen mesh anti -blocking device on the return path, the lower layer collection bin sets up in the lower layer separation bin below, including the entity conveyer belt of horizontal operation. The flattening scraping thin device will viscous or too thick insect sand even spread thin, promote light and heat penetrability and larvae drill out efficiency, the screen mesh anti -blocking device passes through the brush penetration and cleans the conveyer belt screen mesh hole, avoids the screen mesh blockage and leads to the conveyer belt shutdown. The above based on light and heat dual drive's black soldier fly larvae and insect sand separation system can realize the efficient and stable separation of larvae and insect sand.

The application discloses a chimeric antigen receptor with a CD81 costimulatory domain and application thereof. The chimeric antigen receptor with the CD81 costimulatory domain comprises, from N-terminus to C-terminus: (i) an extracellular antigen binding domain; (ii) a transmembrane domain; (iii) a costimulatory domain, wherein the costimulatory domain is a CD81 costimulatory domain; and (iv) a signaling domain. The application connects the C-terminal intracellular domain of the CD81 molecule as the costimulatory domain of the CAR with the signaling domain, successfully constructs a novel CD19-CAR-T cell (81z) with the CD81 as the costimulatory signal. Compared with the existing CD28 or 4-1BB costimulatory domain CAR-T, the CAR-T cell with the CD81 costimulatory domain obviously reduces the release of toxic cytokines after tumor cell stimulation, reduces the release of mononuclear cell inflammatory factors IL-1beta and IL-6 mediated, and has good targeted killing CD19 + tumor cell ability.

The invention relates to the technical field of medicines, and discloses application of gastrodin in preparation of a medicine for preventing and treating radioactive liver injury, and the radioactive liver injury is liver injury caused by ionizing radiation exposure. The gastrodin is found to be capable of effectively intervening a specific mechanism of the radiation-induced liver injury, so that the death rate of mice with the radiation-induced liver injury is remarkably reduced, meanwhile, the core pathological characteristics of hepatic vein occlusion and hepatic fibrosis of the radiation-induced liver injury are reversed, and the radiation-induced liver injury is treated in a more targeted manner. On the other hand, gastrodin is used before radiation exposure, so that serious radioactive liver injury after radiation exposure can be prevented.

This application relates to the field of bio-agricultural breeding technology, and in particular to a method for off-season scallop seedling cultivation and its application. This application utilizes an indoor low-temperature circulating water system and employs a comprehensive environmental and nutritional control strategy involving "artificial low-temperature dormancy – gradient warming for maturation – off-season induced spawning – low-temperature acclimatization transition" to disrupt the natural seasonal rhythm of scallop reproduction. This not only ensures the survival rate of scallops released into the sea but also allows for staggered release times, shortens the cultivation cycle, and reduces summer mortality.

The invention belongs to the technical field of camellia oleifera forest planting ecological intelligent management, and particularly discloses and provides an internet-based camellia oleifera forest oil shaving interaction planting ecological intelligent management platform. The system is based on a constructed camellia oleifera afforestation monitoring module, a camellia oleifera afforestation analysis module, a historical pest and disease information extraction module, a specified period analysis module, a susceptible pest and disease type analysis module, a display terminal, a pest and disease monitoring module, a pest and disease coincidence analysis module, a pest and disease grade analysis module, a management center and a cloud database. According to the method, the survival rate of camellia oleifera forest seedlings is increased by analyzing camellia oleifera forest afforestation environmental conditions, disease and insect pest prevention, actual disease and insect pest conditions and the like, so that farmers can prevent the camellia oleifera forest seedlings in time before the camellia oleifera forest has diseases and insect pests, and the prediction accuracy before the camellia oleifera forest has diseases and insect pests is improved; the economic loss of farmers is further reduced, and resource waste and environmental pollution are reduced.

The invention discloses an acute respiratory distress syndrome (ARDS) prediction method and system based on multi-modal data and deep learning, relates to the technical field of computer-aided diagnosis, and provides a computer algorithm for intelligently predicting the severity of an ARDS patient based on computed tomography (CT) imaging and clinical indexes. Therefore, doctors can be assisted in early intervention and treatment. According to the technical key points, inflammation can be effectively segmented by using a 2.5 D U-Net model and a sub-visual recognition algorithm; according to the method, the ARDS is predicted by introducing an Extreme Graduent Boosting (XGBoost) algorithm after the segmented inflammation features are extracted, so that the ARDS prediction based on the imaging features is realized; the system can shorten the diagnosis time and reduce the death rate, and is of great significance to early diagnosis of ARDS. Experimental results show that the method provided by the invention has high accuracy in predicting the severity of the ARDS, the Dice coefficient of lung inflammation segmentation is 90.2%, and the accuracy of predicting the severity of the ARDS is 86.5%.

This invention discloses an intracellular imaging system for simultaneously detecting UDG and miR-375 expression levels and its applications. The intracellular imaging system uses a tetrahedral DNA nanostructure as a carrier, loading a HAT-UDG probe with an on / off conformation, and coupling it with a split CRISPR / Cas12a detection component for recognizing miR-375. The split CRISPR / Cas12a detection component includes at least Cas12a protein, scaffold RNA, spacer RNA / equivalent split crRNA components, and a fluorescent reporter substrate. This invention is the first to perform a logical AND operation between two key indicators: UDG enzyme activity and miR-375 expression level. The system can only ultimately trigger a complete fluorescence signal when both are simultaneously highly expressed. This multi-target synergistic activation design constructs a precise molecular recognition logic gate, enabling extremely accurate differentiation between prostate cancer cells (RV-1) and normal cells (293T), as well as other cancer cells (5637) that highly express UDG, fundamentally avoiding misjudgments caused by fluctuations in a single indicator or non-specific expression, resulting in extremely high imaging specificity.

The invention belongs to the technical field of liquid fertilizers, and particularly relates to a method for producing a vegetarian liquid fertilizer by using livestock and poultry manure sewage and a field returning process, the method for producing the vegetarian liquid fertilizer by using the livestock and poultry manure sewage comprises the following steps: preparing a refined base solution from the livestock and poultry manure sewage, selecting at least one planting pioneer strain with a rhizosphere planting advantage marker, and carrying out field planting on the selected planting pioneer strain; and forming a synthetic microbiome with 2-4 functional strains, inoculating the synthetic microbiome into the refined base solution in the step 1, and carrying out gradient adaptive fermentation. The phytohormone liquid fertilizer with extremely high targeting property can be prepared, the fertilizer efficiency accuracy is improved, the phytohormone liquid fertilizer directly acts on a plant root system, nutrient waste is reduced, the phenomenon that functional thalli are ineffectively consumed or die in a non-rhizosphere environment is reduced, the functional thalli are intensively released and planted in a rhizosphere area, the rhizosphere colonization number of target flora is greatly increased, and the yield of the target flora is increased. The influence on normal metabolism of non-target microbial communities is reduced, and the damage to soil native micro-ecology is reduced.

A traditional Chinese medicine composition, a pharmaceutical preparation and a use for treating and / or preventing sepsis. The traditional Chinese medicine composition is made of the following traditional Chinese medicine raw materials: honeysuckle 10-20 parts, forsythia 10-20 parts, red peony root 10-20 parts, salvia miltiorrhiza 7-13 parts, cortex moutan 7-13 parts and licorice 4-8 parts by weight. The traditional Chinese medicine composition can significantly reduce the mortality of sepsis mice, prolong the survival time of sepsis mice, reduce the expression level of inflammatory factors TNF-alpha, IL-6 and IL-1 beta in the serum of sepsis mice, reduce the damage degree of target organs lung, liver and kidney, and protect the organ function of lung, liver and kidney by inhibiting the levels of MPO, ALT, AST and CRE.

This invention relates to the field of livestock breeding equipment technology, specifically to an automatic nursing machine for suckling rabbits. It includes a frame, several assemblies of brooding boxes, a milk supply and nursing assembly, and a drive assembly. The frame includes left and right uprights, with several half-shafts fixed to the inner sides of each upright. Each half-shaft has a rotating sprocket fixed to it, and rotating chains are mounted on the rotating sprockets on the left and right uprights. The brooding box assembly includes a brooding box body and a rotating shaft, with both ends of the rotating shaft rotatably connected to the rotating chains on the left and right sides, respectively. The milk supply and nursing assembly includes a milk delivery pipe, a milk supply pipe, and a sucking nipple. The drive assembly includes a motor reducer and a power shaft. The application of this automatic nursing machine can greatly save labor in the management and operation of suckling rabbits, reduce the cost of raising and managing rabbits, and significantly improve the production efficiency of rabbit farming.