In scanned optical systems such as
confocal laser microscopes wherein a beam of light is focused to a spot in a specimen to excite a fluorescent species or other excitable species in the spot, the
effective size of the excitation is made smaller than the size of the spot by providing a beam of light of
wavelength adapted to quench the excitation of the excitable species, shaping this second beam into a pattern with a central intensity minimum, and overlapping this central minimum with the central intensity maximum of the focused spot, so that within the spot the intensity of
quenching light increases with distance from the center of the spot, thereby preferentially
quenching excitation in the
peripheral parts of the spot, and thereby reducing the
effective size of the excitation and thus improving the resolution of the
system. In the preferred embodiment of the present invention, the central minimum of
quenching light is narrowed further by creating the pattern of quenching
radiation in the specimen by imaging onto the focal plane a plurality of pairs of sources of quenching light, arrayed at the vertices of a regular, even-sided polygon, the center of which is imaged in the specimen on the central maximum of exciting
radiation, and such that the two members of each pair are on opposite vertices of the polygon and emit light mutually coherent and out-of-phase, and the light emitted by different pairs is incoherent with respect to each other. Optical fibers conduct both excitation light and quenching light to the
microscope body, preventing transmission of vibration from the
laser apparatus to the
microscope, thereby avoiding degradation of resolution.