4216 results about "Fluorescence" patented technology

A federated distributed computational system enables secure oncological therapy optimization through robotic integration. The system establishes a distributed graph architecture with secure communication channels connecting computational nodes, implementing encryption protocols for cross-institutional data exchange. Each node contains processing capabilities for fluorescence-guided imaging, uncertainty quantification, and expert knowledge integration while maintaining hierarchical knowledge graphs of oncological biomarkers, interventions, and outcomes. The system coordinates domain-specific knowledge through token-space communication and implements an advanced robotic integration system for surgical interventions using spatiotemporal tumor mapping, multi-modal fluorescence imaging, surgical robot coordination, and space-time stabilized mesh management. Key capabilities include wavelength-specific multi-modal fluorescence detection, combined epistemic and aleatoric uncertainty estimation, tensor-based data integration with adaptive dimensionality control, and light cone search for adaptive treatment optimization—all while maintaining strict privacy controls.

The invention relates to a saline intrusion measurement method, system and equipment based on dynamic ship motion conditions and a medium, and aims to solve the technical problem that the salinity inversion precision is reduced due to spatial-temporal dislocation between particle image velocity measurement flow field data and plane laser-induced fluorescence concentration field data caused by ship six-degree-of-freedom motion. According to the method, future poses are predicted by collecting ship motion parameters and environment disturbance parameters, and space-time compensation parameters are generated in combination with a static calibration matrix; performing three-dimensional coordinate transformation and time synchronization on synchronously acquired original flow field and concentration field data by applying compensation parameters, and eliminating space-time deviation caused by ship movement; a three-dimensional salinity distribution field is calculated based on registration data fusion flow field diffusion characteristics and concentration field distribution; and meanwhile, the pose of the measuring equipment is dynamically adjusted through the closed-loop controller so as to optimize the data acquisition quality. According to the method, ship motion interference is effectively inhibited, and accurate characterization of saline invasion frontal surface spatial characteristics is guaranteed.

The invention discloses a detection method of an ocean magnetic field sensor based on a diamond NV color center. The detection method comprises the following steps: S1, calibrating a base line; s2, alternately measuring fluorescence between two single quantum resonance frequencies, and obtaining a difference to realize DQ reading; s3, three-axis compensation current is adjusted based on closed-loop control, and the NV working point is maintained at zero magnetization bias; s4, obtaining background magnetic field change through digital demodulation and adaptive filtering; and S5, calculating a gradient based on the spatial difference of the multi-point light spots, judging the existence and trajectory of the underwater vehicle in combination with a threshold value and a spectrum matching method, calibrating the threshold value according to the magnetic field characteristics of the existing underwater vehicle, adopting short-time Fourier transform signal spectrum, comparing the threshold value with known submarine characteristic spectrum data, and prompting if the similarity exceeds a set threshold value. According to the detection method, the measurement precision and the anti-interference capability can be improved, and the method is suitable for real-time monitoring of weak magnetic anomalies (such as magnetic moment disturbance of an underwater vehicle).

The invention provides a microscopic automatic focusing method and system based on image gray histogram characteristics, and the method comprises the steps: collecting an image sequence under different focal lengths, carrying out the fuzzy processing, extracting a gray histogram of each frame of image, and calculating the peak position and full width at half maximum of the histogram as the evaluation characteristics of the image definition; calculating the variance of each feature and automatically allocating a weight according to the relative response degree; and finally, comprehensively evaluating the image definition through a weighted definition scoring function, and selecting the focal length corresponding to the image with the optimal score as the optimal focusing position. The method is based on the global features of the gray histogram, is high in anti-noise capability, is adaptive to different imaging scenes through weight adaptive adjustment, is low in calculation complexity, supports real-time focusing, is especially suitable for high-noise fluorescence microscopic imaging scenes, is high in system portability, is low in operation threshold, and effectively improves the accuracy and stability of microscopic automatic focusing.

The invention discloses a transformer oil leakage detection method and device based on polarization-ultraviolet fusion imaging, and the device comprises a housing, a support, a camera, an environment induction device, an overall controller, a power supply, a polaroid rotating wheel assembly, a lens seat assembly, and an image processor. And the motor driver, the illumination intensity sensor, the ultraviolet irradiance sensor, the image processor and the power supply are electrically connected with the integral controller respectively. According to the invention, a multi-angle polarization image and a non-polarization image in the same field of view can be acquired, and then polarization characteristics contained in an oil stain fluorescence signal are extracted and effectively distinguished from non-polarization characteristics of background light; in the daytime mode, the transformer oil leakage detection device can identify a target area with relatively high polarization intensity, and through image fusion enhancement processing, non-target background signals are significantly suppressed, and the fluorescence significance of an oil leakage characteristic area is improved.

Methods and devices for analyzing a sample from a subject are provided. A device includes one or more coherent light sources and one or more partially coherent light sources. The light sources are configured to emit first and second wavelengths of electromagnetic radiation towards an imaging chamber configured to hold blood cells. The light sources are aligned to illuminate a single common area of the imaging chamber. The device includes optics positioned to receive light from the imaging chamber, and a detector in optical communication with the optics. The detector is configured to detect fluorescence emission and backscatter from blood cells when present in the imaging chamber. The device includes mixers / unmixers configured to replicate and mix first and second signals representative of the responses to the first and second wavelengths after interacting with the plurality of blood cells to generate spectro-spatial responses.

The utility model relates to the field of microfluidic detection and fluorescence immunoassay, in particular to a portable biochemical blood coagulation and fluorescence immunoassay integrated analysis device, which comprises a shell, a power supply arranged in the shell, two support plates arranged in the shell, a fluorescent bracket fixedly connected to the upper ends of the support plates, a support arranged in the shell, a lamp source arranged at the upper end of the support, and a light source arranged at the lower end of the lamp source. A microfluidic detection module is mounted in the support, a fluorescence immunodetection module is mounted at the upper end of the fluorescence bracket, an optical module is arranged in the fluorescence immunodetection module, and a bar code scanner for confirming information of a detection chip or a reagent strip is arranged on one side of the optical module; compared with the traditional detection equipment with a single function, the double detection functions of biochemistry and fluorescence immunity are realized, the detection efficiency and comprehensiveness are greatly improved, meanwhile, the kit also supports the simultaneous detection of three reagent strips with different specifications, and the flexibility and convenience of the detection are further enhanced.

A neurosurgery system for probing brain tissue of a patient for tumorous tissue. The system including a suction tool, an excitation source, an optical instrument, and a controller. The suction tool including a suction cannula defining a lumen, an optical fiber configured to transmit fluorescence emitted by the brain tissue; and an indicator configured to selectively emit visible light. An excitation source is configured to emit an excitation light having a wavelength to induce the fluorescence in the tumorous tissue. The optical instrument is coupled to the optical fiber. The optical instrument configured to convert the fluorescence emitted by the brain tissue and transmitted by the optical and configured to determine that the brain tissue is tumorous based on the electrical signal and activate the indicator based on the determination.

The invention discloses an all-optical three-dimensional scanning confocal fluorescent microscopic imaging device and an implementation method thereof. According to the invention, through a deep learning driven adaptive regulation and control method, CNN is adopted to process spatial distribution data and dynamically regulate and control a phase hologram and the light intensity and phase compensation of a laser, so that the focal point of exciting light is subjected to aberration-free axial displacement, a bidirectional parallel optical scanning track of a two-dimensional scanning system is optimized, and all-optical three-dimensional scanning is realized; lSTM and TCN are combined to obtain a time sequence dependency relationship, a laser light source, an adjustable diaphragm, an electric focus-adjustable lens and a photoelectric detector are integrally controlled, efficient synchronization and automatic operation and high-speed axial focusing adjustment are realized, errors and time sequence mismatch are eliminated, optical characteristics of different samples and environmental interference are automatically adapted, and high-quality imaging is kept. The robustness and the applicable scene range of the system are improved; the method is used for model biological embryo real-time tracking, intracellular signal molecule dynamic visualization, cell membrane protein migration and aggregation observation and intracellular organelle interaction tracking.

The invention discloses an intelligent sorting method and device for a positive electrode material of a retired lithium ion battery based on a spectrum method, and the device is connected in series with a pretreatment module, a cleaning module, an identification module and a sorting module through a conveying belt in a supporting module to form an automatic sorting assembly line. Pre-treating the retired battery to obtain a clean positive plate; an X-ray fluorescence spectrophotometer in the recognition module collects the spectrum of the positive plate and transmits the spectrum to an industrial personal computer, and the spectrum is preprocessed and then input into a classifier library; the classifier library comprises a main classifier and an increment classifier based on element and spectral shape dual channels, if the output confidence reaches a preset threshold value, the material category is judged, otherwise, the spectrum is temporarily stored as an unknown spectrum; after unknown spectrums are accumulated to a set number, new categories are identified through a clustering algorithm, and a lightweight dichotomy increment classifier is trained and added into a classifier library; and the sorting module drives the hydraulic pushing hand to sort the positive plates according to the identification result. Through cooperative evolution of the main classifier and the increment classifier, the sorting precision is effectively improved.

The utility model discloses a detection device and a control system for settlement of a lap joint surface of a new roadbed and an old roadbed, and relates to the technical field of road engineering monitoring, the detection device comprises a first support plate, a second support plate, two solar panels, two vibration sensors and two storage batteries, a placing box is mounted on the supporting rod, a laser displacement sensor and an industrial camera are mounted in the placing box, a detection plate is mounted on the second supporting plate, and a fluorescent graduated scale is mounted on the detection plate; according to the utility model, the traditional threaded rod and threaded cylinder adjusting mode is replaced by optimizing the adjusting mechanisms, the problems that a threaded structure is easy to wear and block and the manual operation efficiency is low are effectively solved, and a composite monitoring mode of the laser displacement sensor, the industrial camera and the fluorescent graduated scale is adopted, so that the monitoring precision is improved. And a multi-dimensional auxiliary assembly is combined to improve the reliability of the system.

The invention relates to the technical field of oil stain detection, and discloses an oil-filled equipment oil stain detection method based on fluorescence reflection. The method comprises the following steps: carrying out cleaning pretreatment on a to-be-detected surface; an ultraviolet light source is utilized to excite the oil stain to generate fluorescence; collecting a fluorescence image through an imaging device with a filter; performing noise reduction and edge processing on the image to extract an oil stain area; and finally judging the existence and type of oil stain and estimating the thickness according to image features. According to the invention, the fluorescence characteristic of the oil stain is utilized, no fluorescent agent needs to be added, and pollution-free, high-precision and high-efficiency non-contact detection is realized.

The invention discloses an underwater magnetic field measuring device based on a diamond NV color center. The underwater magnetic field measuring device comprises a diamond NV color center module, an incident laser excitation module, a multi-path optical coupling module, a microwave excitation module, a fluorescence acquisition and photoelectric conversion module, a signal processing and control module and a magnetic field module, the multi-path optical coupling module is used for dividing an optical fiber into multiple paths and collecting fluorescence generated by the diamond block; the microwave excitation module is used for switching between two single-quantum resonance frequencies to drive an NV color center to realize double-quantum DQ reading; and the signal processing and control module comprises an FPGA (Field Programmable Gate Array) processing unit of a field programmable logic array chip. According to the invention, a dual-quantum DQ working point of a diamond NV color center is used as a measurement channel, common-mode influence of D is removed through differential measurement, the problem of D drift caused by temperature drift, stress and material aging is physically and obviously inhibited, and the device can still realize ultrahigh-sensitivity measurement in a complex underwater environment.

The invention provides a portable micro-beam X-ray fluorescence rapid detection method and system based on digital signal processing. The method comprises the following steps: firstly, constructing a micro-beam excitation parameter adaptive to a field environment, secondly, driving a micro-beam X-ray to irradiate a to-be-detected sample by using the micro-beam excitation parameter, synchronously obtaining an original fluorescence signal disturbed by the environment, then taking a dynamic adjustment amount as a calibration reference, and fusing the calibration reference and the original fluorescence signal to generate a combined data stream; then carrying out cooperative processing on the combined data stream through a digital signal processing technology, separating an environment disturbance component from a target fluorescence component, and finally generating a sample surface element distribution map based on the target fluorescence component. According to the technical scheme provided by the invention, the problems of morphology misalignment and dynamic noise interference caused by passive noise reduction design are solved, and the element detection reliability of complex morphology samples (such as soil blocks and cultural relic sections) is also improved.

The invention discloses a wide-dynamic-range diamond NV color center magnetic sensing method and system, and the method comprises the steps: (1) calibrating the initial resonant frequency and magnetic sensing coefficient of a diamond NV color center in an initial magnetic field environment; (2) in a to-be-measured magnetic field environment, a microwave generator applies frequency-modulated microwaves to the diamond NV color center with the current resonant frequency as the center frequency; demodulating the fluorescence signal in real time by using a phase-locked detection technology to obtain an error signal; calculating the detuning amount of the center frequency in real time according to the error signal and the magnetic sensing coefficient, and further calculating the variable quantity of the magnetic field intensity; meanwhile, the detuning amount is fed back to a microwave generator to track the resonance frequency of the diamond NV color center; and (3) continuously and repeatedly executing the step (2) to realize wide-dynamic-range magnetic field measurement. The method has the advantages of wide dynamic range, high detection capability on strong and weak field mixed signals in a complex application scene and the like while giving consideration to the magnetic sensing sensitivity.

A geological analysis system, device, and method using x-ray fluorescence and spectroscopy are provided. The geological analysis system includes a sample tray which holds the geological sample materials, and sensors including an X-ray fluorescence (XRF) unit and spectrometer. The sample tray includes chambers formed in an upper surface, ports, and passages, each providing communication between an interior of a chamber and an interior of a port. The ports are configured to be attachable to vials. The system positions the sample tray with respect to the sensors for sensing one or more properties of geological sample materials in the sample tray.

The invention discloses a multi-scale performance evaluation and optimization method and device for well cementation cement stone, and relates to the technical field of oil exploitation, the well cementation cement stone is subjected to nanoindentation test and scanning electron microscope microstructure test, and a mechanical property-microstructure test result is obtained; performing electronic computer tomography and mercury injection test on the well cementation cement stone, and constructing a pore network three-dimensional model; performing triaxial mechanical property test, thermogravimetric analysis and thermal expansion test on the well cementation cement stone to obtain a thermal-mechanical coupling test result; performing X-ray diffraction and X-ray fluorescence spectrum analysis on the well cementation cement stone to obtain mineral phase information and element information of the cement stone; according to the method, a mechanical property-microstructure test result, topological parameters, a thermal-mechanical coupling test result, cement stone mineral phase information and element information are fused, an optimal design scheme for target well conditions is generated, multi-dimensional performance testing and characterization are achieved, and an accurate basis is provided for well cementation cement stone optimal design and quality control.

The invention relates to the technical field of quantum sensing, and discloses a quantum magnetometer device based on diamond NV color center photoelectric detection magnetic resonance. Wherein the optical path module performs optical excitation and imaging positioning on a diamond sample through a laser and an objective lens. The circuit module integrates a microwave source, a lock-in amplifier, a low-noise current amplifier and a programmable power supply, applies a microwave field and bias voltage to a microwave antenna and an interdigital electrode on the surface of a sample respectively, and collects sample dependency photocurrent signals. And the operation module realizes automatic control and data processing of the system. According to the device, NV color center spin state information is converted into a photocurrent signal through a photoelectric detection magnetic resonance mechanism, an amplitude or frequency modulated signal is demodulated by using a lock-in amplification technology, information of a detection magnetic field is obtained through a high signal-to-noise ratio photoelectric detection magnetic resonance spectrum, and the sensitivity of the magnetic field is evaluated. Magnetic resonance signals are detected in an all-electricity mode, a complex optical system of traditional fluorescence detection is simplified, and the device has the advantages of being compact in structure, easy to integrate, high in measurement sensitivity and the like.

The invention provides a colorimetric / fluorescent dual-mode nano-enzyme sensor, a preparation method thereof and application of the colorimetric / fluorescent dual-mode nano-enzyme sensor in formaldehyde detection. The sensor comprises a metal organic framework nano-enzyme AIE-MOF coated PCN-222 loaded with an AIE-MOF material, hydrogen peroxide and a chromogenic substrate o-phenylenediamine OPD; the metal organic framework nano enzyme PCN-222 in the AIE-MOF-coated PCN-222 can oxidize colorless OPD into yellow 2, 3-diaminophenazine DAP, an ultraviolet absorption peak is generated at the position of 450 nm, a strong fluorescence emission peak is generated at the position of 560 nm, the AIE-MOF-coated PCN-222 has a strong fluorescence emission peak at the position of 446 nm, and the AIE-MOF-coated PCN-222 and a fluorescence signal of the DAP can form a ratio fluorescence sensor. According to the invention, the excellent catalytic ability of the nano-enzyme and the luminescence characteristic of AIE-MOF are integrated in one system, so that high-sensitivity formaldehyde detection is realized.

The invention discloses a temperature control system and method of a micro-fluidic chip, and relates to the technical field of micro-fluidic temperature control, and the method comprises the following steps: in the manufacturing process of the micro-fluidic chip, embedding topological insulator nanowires into a substrate in a directional arrangement mode to form a pre-locking array, and meanwhile, generating an anisotropic heat conduction network under the continuous action of a sound-magnetic field, a topological vortex invariant is calculated, and a heat conduction weight coefficient is generated; arranging a fluorescent layer on the surface of a micro-reaction cavity of the anisotropic heat conduction network, collecting a fluorescence lifetime attenuation curve in real time, and calculating a transient temperature value and a temperature change rate according to a heat conduction constraint variation model and a heat conduction weight system; nonlinear feed-forward compensation power is calculated according to the transient temperature value and the temperature change rate, feedback compensation power is calculated in combination with a deviation field of the target temperature and the real-time temperature, and a compensation power instruction is generated; according to the method, the thermal pilot frequency domain filtering function is constructed through the compensation power instruction, so that the uniformity and response speed of thermal response are improved.

The invention relates to the technical field of medical image information processing, discloses an ICG fluorescence image and MRCP three-dimensional model registration fusion display method and system, effectively solves the problems of deep biliary tract structure blurring and form distortion caused by liver tissue scattering effect in intraoperative fluorescence imaging, uses a preoperative model as anatomical prior, and improves the accuracy of the intraoperative fluorescence imaging. A viewpoint correlation optical path depth field is constructed through reverse ray tracing, a spatial variation point spread function is generated accordingly, physical reverse recovery of scattering halo in a fluorescence image is achieved, non-specific background noise such as vascular leakage is effectively inhibited in combination with topological gravitational constraint of a central skeleton, and the detection accuracy is improved. The real pipe diameter and the center line position of the deep bile duct are accurately recovered, non-rigid registration correction is performed on the three-dimensional model based on the recovered high-fidelity image, and projection errors caused by respiratory movement and soft tissue deformation are eliminated.

The invention provides a light source multiplexing diamond NV color center wide field detection system and an illumination detection method. The system comprises a diamond containing an NV color center, an exciting light module, a first double-color sheet, an objective lens, a second double-color sheet, a wide field imaging module, an illumination imaging module and a microwave module, exciting light generated by the exciting light module irradiates the first double-color piece, is guided to the objective lens, and irradiates the diamond after being transmitted by the objective lens so as to excite the NV color center of the diamond to generate fluorescent light; the objective lens is of an infinity type; the exciting light is irradiated to a sample to be detected after being transmitted by the diamond; fluorescent light and exciting light from a sample to be detected are collected by the objective lens, then transmitted to the first double-color piece and guided to the second double-color piece through the first double-color piece, the second double-color piece guides the fluorescent light to the wide-field imaging module for imaging, and the exciting light in the second double-color piece is guided to the illumination imaging module for imaging. Illumination can be achieved in the exciting light working state, exciting light switching operation in the prior art is avoided, and efficiency can be remarkably improved.

The invention relates to the technical field of biomass fibers, in particular to a modified biomass fiber for a resin anticorrosive coating as well as a preparation method and application of the modified biomass fiber, and the modified biomass fiber is a biomass fiber with a rare earth complex anchored on the surface; the rare earth complex is formed by combining rare earth metal ions serving as central ions with organic ligands through coordinate bonds; rare earth ions and organic ligands are grafted to the surfaces of the pretreated biomass fibers through chemical reaction, so that the binding capacity of the biomass fibers in resin can be improved, and the biomass fibers are endowed with fluorescence characteristics; and moreover, the biomass fibers subjected to fluorescence modification are doped into the resin coating, so that the integrity of the coating can be self-detected under ultraviolet light, and the effect of monitoring the resin coating in real time can be achieved.

The invention relates to the technical field of analysis and detection, in particular to a fluorescent switch sensor based on quantum dot-nano porphyrin and application. The specific technical scheme is as follows: the fluorescent switch sensor is constructed by utilizing cadmium telluride quantum dots modified by mercaptosuccinic acid and a 5, 10, 15, 20-tetra (4-nitrophenyl) porphyrin nano material modified by a cationic surfactant hexadecyl trimethyl ammonium bromide. And the method has a good enrichment effect on zearalenone. The sensor has high sensitivity, high selectivity and anti-interference performance, can accurately analyze trace zearalenone in actual grain samples (corn, rice, soybean and the like), and has the advantages of simplicity in operation, low detection limit, high sensitivity, low cost and the like.

The embodiment of the invention provides a switch cabinet partial discharge quantum sensing monitoring device. The device is applied to the technical field of power equipment monitoring, and comprises a light path regulation and excitation module which is used for generating and beam-splitting detection laser of a quantum sensor coupled to each Rydberg atom; the quantum electromagnetic induction module acts on Rydberg atoms and a partial discharge electromagnetic field, and fluorescence or absorption spectrum characteristics of atomic energy level transition are changed; extracting a weak discharge signal, and converting the weak discharge signal into an analyzable electric signal; the signal processing and diagnosis module is combined with spatial information acquired by a quantum sensor of doydberg atoms in a distributed manner, and a time-frequency analysis and pattern recognition algorithm is adopted to extract discharge characteristic quantity; partial discharge positioning, type identification and insulation state evaluation are realized based on the parameters, and a real-time monitoring result and early warning information are output. In this way, through deep fusion of quantum sensing and advanced signal processing, a high-precision and high-reliability technical solution is provided for power equipment state monitoring.

The invention discloses a magnetic sensor, a sensing measurement system and a magnetic field measurement method, and relates to the technical field of magnetic field measurement, the magnetic sensor comprises an excitation light source, a light path collection assembly, a fluorescence detector and a shielding shell, and the excitation light source, the light path collection assembly and the fluorescence detector are fixedly arranged in the shielding shell in sequence; the light path collection assembly comprises an NV color center diamond, a magnetic field bias module and a fluorescence convergence assembly, the magnetic field bias module can provide a bias magnetic field for the NV color center diamond, the NV color center diamond can emit fluorescence under the action of the exciting light and the bias magnetic field, and the fluorescence convergence assembly can converge the fluorescence emitted by the NV color center diamond to collect the fluorescence emitted by the NV color center diamond. The fluorescence detector can receive the fluorescence converged by the fluorescence convergence assembly, and can transmit a light current signal obtained by detecting the fluorescence to the signal acquisition system; according to the invention, volume miniaturization can be realized conveniently, scene adaptability, anti-interference capability, precision stability and reliability are improved, and use cost is reduced.

The invention discloses a living cell super-resolution imaging method and system based on self-supervised learning. The method comprises the following steps: S1, obtaining a fluorescence microscopic image through a standard fluorescence microscopic system; s2, for the acquired single noise image, generating positive and negative sample pairs of self-supervised training data through an autonomously designed and optimized self-supervised strategy; s3, constructing a front denoising neural network and a rear deconvolution network, and performing network training; and S4, inputting a newly obtained noise image into the trained front denoising network to obtain a denoised image, and inputting the denoised image into the rear deconvolution network to obtain a final super-resolution reconstructed image. According to the invention, the standard fluorescence microscopic system and the self-supervised denoising processing module are combined, so that high-quality denoising and super-resolution reconstruction of the fluorescence microscopic image can be realized in a low-photon signal scene.

The embodiment of the invention discloses a scanning NV probe microscope which comprises an atomic force microscopy system used for detecting surface topography information of a sample and an optical detection magnetic resonance system used for detecting surface magnetic field distribution information of the sample, and the atomic force microscopy system comprises a vibration arm and a diamond probe. The optical detection magnetic resonance system comprises an NV color center, a transition excitation light path and a fluorescence detection light path; a sample to be scanned and the transition excitation light path are respectively positioned on two opposite sides of the diamond probe, and the transition excitation light path and the fluorescence detection light path are positioned on the same side of the diamond probe; a first metal coating film is arranged on one side, close to the sample, of the diamond probe and is used for blocking surface fluorescence of the sample. According to the scanning NV probe microscope provided by the embodiment of the invention, the metal coating is arranged at the bottom of the diamond probe to block stray fluorescence from the surface of the sample, so that the problems of low magnetic field imaging signal-to-noise ratio and limited measurement precision of the existing scanning NV probe microscope are solved.

The invention provides a linear optical tweezer astigmatism field regulation and control method based on an optical transmission matrix. The design method comprises the following steps: establishing a 4 * 4 optical matrix for generating a linear optical tweezer astigmatism field and a transmission equation; analyzing the regulation and control rule of the parameters of the optical element on the astigmatism characteristics; collecting a fluorescence slice image of the initial astigmatic light field through an axial tomography scanning method; reconstructing the spatial distribution of the initial image light field; judging whether the light field accords with a preset astigmatism characteristic or not, if not, determining the astigmatism characteristic to be optimized, and adjusting element parameters according to a regulation and control rule; and acquiring the adjusted image of the astigmatism light field again, reconstructing the image, and continuously performing judgment iteration until the image accords with the preset astigmatism characteristic, thereby realizing regulation and control of the astigmatism light field. Matrix optics is used in optimization of a linear optical tweezer image dispersion light field optical system, the limitation of traditional trial and error design is avoided, the method can be applied to the fields of light control technology, light sheet fluorescence microscopic imaging and the like, and the performance of the optical system is improved.

The invention discloses an air microorganism aerosol alarm sampling system and method and an unmanned aerial vehicle system. A main controller receives a current position positioned by a GPS positioning module, and when the current position is in a to-be-detected area, an air pump and an alarm sampling optical module are started to pump air into the alarm sampling optical module; the alarm sampling optical module samples microbial particles in the air to form a fluorescence signal, the main controller analyzes the aerosol concentration according to the fluorescence signal and identifies whether the aerosol is a biological aerosol, when the aerosol concentration exceeds a set threshold value or the aerosol is identified as the biological aerosol, the alarm is controlled, and meanwhile, the sampling module is controlled to be started; the main controller controls the liquid supplementing peristaltic pump to start, the sampling liquid in the sampling liquid bottle is pumped into the sampler, then the motor driver is controlled to drive the fan to start to enable air to enter the sampler for sampling, only the sampling peristaltic pump is controlled to start after sampling is completed, and the sample liquid in the sampler is conveyed into the sample bottle through the three-way pipe. The collection of the microbial aerosol particles is completed.