813 results about "Biological tissue" patented technology

A robotic surgical system includes one or more robotic actuators configured to interact with biological tissue during a surgical procedure. A plurality of sensors include at least one of fiber Bragg grating sensors, piezoelectric strain sensors, or magnetostrictive sensors to capture real-time mechanical, elasticity, or deformation data from biological tissues. deep learning engine trained on a dataset comprising tissue mechanical responses across multiple tissue types, pathological states, and patient demographics. Pre-contact predictive adjustment profiles are generated for anticipated tissue interactions using preoperative imaging data registered to intraoperative coordinates. Intraoperative deviations are detected from predicted mechanical behavior and autonomously recalibrate actuator forces. Upcoming surgical maneuvers are anticipated based on prior task sequences and adjust actuator stiffness or damping properties in preparation for anticipated contact. An emergency override of actuator forces is provided via an anomaly detection module when real-time sensor data deviates beyond a threshold from the predicted safe mechanical response range. A feedback loop iteratively refines the deep learning engine during the procedure using supervised learning updates, anomaly detection, and reinforcement learning strategies. The reinforcement learning model is optionally shared across procedures to optimize distributed actuator force patterns for minimizing localized and cumulative tissue stress.

The invention discloses a multi-frame photoacoustic image reconstruction method based on optical flow alignment and depth feature fusion. The method comprises the following steps: S1, obtaining photoacoustic signal data; s2, reconstructing a photoacoustic cross-sectional image; s3, performing optical flow calculation and image alignment; and S4, training the deep feature fusion network. According to the method, through optical flow motion correction and a potential space learning mechanism, space-time information and complementary features in the aligned multiple frames of images are dynamically integrated, complementary information in the multiple frames of images is adaptively fused, noise is suppressed, and finally reconstruction of high signal-to-noise ratio and high spatial resolution images of biological tissues is achieved. Experimental results show that the method can significantly improve image quality, recover image distortion and detail loss caused by motion and noise, and provide a new effective scheme for promoting robust clinical application of a photoacoustic imaging technology.

The invention relates to a three-dimensional spiral high-density neural electrode and a preparation method and application thereof. The three-dimensional spiral high-density neural electrode comprises a probe structure and a plurality of electrode sites. The probe structure is formed by curling a planar flexible electrode precursor, and at least one end of the probe structure is provided with a spiral outer surface of a three-dimensional spiral line structure. Electrode sites are distributed along a spiral path, have a size of 5-1000 [mu] m, and are used for contacting biological tissues. On the planar flexible electrode precursor, electrode sites are arranged on one or more straight lines forming an inclined angle alpha with the axial direction of the probe, and the spatial distribution is matched with the edge. Compared with the prior art, the method has the advantages that the constraint of a traditional wiring mode is broken through, the integration of high-density three-dimensional channels is realized under a micro size, and a new generation of solution is provided for a high-precision brain-computer interface, deep brain stimulation and a three-dimensional electroencephalogram.

The invention belongs to the technical field of biomedical tissue engineering materials, and particularly relates to injectable composite plastic gel and a preparation method thereof. The injectable plastic gel provided by the invention is formed by suspending and compounding the cross-linked sodium hyaluronate gel particles and the biological tissue extracellular matrix particles, the two material particles in the injectable composite plastic gel are uniform in size and controllable in size, other toxic catalysts do not need to be added in the preparation process, and the injectable plastic gel is easy to prepare. The collagen / elastin composite material is rich in collagen and elastin, and shows good biomechanical properties. Meanwhile, the composite plastic gel contains a plurality of bioactive factors, and after the composite plastic gel is injected and filled into a body, the composite plastic gel is slowly degraded and can promote the generation and ingrowth of autologous fat cells and microvessels, so that the balance of degradation and remodeling is achieved. The preparation method of the injectable plastic gel provided by the invention can be widely applied to the fields of plastic surgery, medical beauty and the like.

The utility model relates to the technical field of fixation and preservation of biological tissue samples, and provides an embedding mold for multiple frozen tissues, which comprises a base, a plurality of clamping pieces, a plurality of clamping pieces and a plurality of clamping pieces, and is characterized in that the top surface is provided with a pair of centrosymmetric supporting blocks; the sleeve is detachably arranged outside the pair of supporting blocks in a sleeving manner; wherein the pair of supporting blocks are arranged at an interval of 180 degrees, and the outer side walls of the supporting blocks are attached to the inner side wall of the sleeve in a transition fit mode; the sleeve is as high as the supporting block, and an accommodating cavity is defined by the inner side wall of the sleeve and is used for embedding the plurality of strip-shaped frozen tissue blocks in a vertical form. The containing cavity formed in the sleeve is changed into a columnar shape with enough depth from an existing flat shape with limited depth, the problems that the tissue structure is incomplete and an OCT embedding medium overflows are directly solved, the edge of a tissue combined block is smooth, slicing is facilitated, meanwhile, a plurality of strip-shaped frozen tissue blocks are embedded in a vertical mode, and the working efficiency is improved. The height of the tissue block is unified, the size of the tissue block is fixed, and the problems that sample loss is large and tissue arrangement is too close to the edge are solved.

The application provides application of SEBS in implanting a biological electrode base. The biological electrode based on the SEBS base can be implanted in biological tissues, and is used for continuously or discontinuously analyzing physiological parameters of the biological body. Compared with other flexible bases, the SEBS base has the characteristics of being thinner and more flexible, can reduce the interference of body movement on the electrical signal, reduce the volume range of the implanted tissue and reduce the inflammatory reaction, thereby improving the measurement accuracy and long-term stability, is expected to meet more implantation site requirements when used for implanting a sensor, and has a good application prospect.

The invention discloses a single cell space transcriptome data analysis method, device and system, and a storage medium. The method comprises the following steps: acquiring single cell space transcriptome data; the spatial transcriptome data comprises a gene expression map and spatial site coordinates; constructing a graph structure considering gene expression similarity and spatial position continuity on the basis of the data, and performing data representation on a gene expression graph by using an adversarial auto-encoder; in combination with a graph neural network, robust potential characterization is learned, training loss is constructed through reconstruction of a gene expression map, and meanwhile, a clustering prediction result of spatial sites is obtained in combination with mcluster clustering. By adopting the technical scheme of the invention, the spatial clustering analysis with higher precision can be realized, and the key spatial functional region in the biological tissue can be identified.

The invention discloses a biological scaffold viscoelasticity evaluation method and related equipment, and aims to overcome the defects of an existing biological scaffold viscoelasticity test method and biological tissue suitability quantitative evaluation. The method comprises the following steps: determining a target implantation part, and acquiring force relaxation experimental data of a biological scaffold by using an atomic force microscope; judging a contact point of the probe and the bracket based on the data; determining the moment when the force signal is stable after the support is compressed to the preset deformation according to the contact point as a force relaxation starting point; calculating the characteristic time when the force value is attenuated to half by taking the starting point as a timing starting point as a microscopic viscoelasticity index; and finally, realizing quantitative evaluation of viscoelasticity suitability by comparing the characteristic time of the stent and the target tissue.

The invention provides a tissue high-resolution mass spectrum imaging technology based on light-driven expansion hydrogel, and belongs to the field of mass spectrum imaging. The technology comprises preparation of light-driven hydrogel, preparation of a light-driven gelation biological sample, digestion and expansion of the gelation biological sample and mass spectrum imaging of the expanded gelation biological sample. The gelation process of the light-driven expansion hydrogel is not inhibited by oxygen, the gelation time is short (only 3 minutes), the gel thickness can be controlled by controlling the addition amount of a gel monomer solution, and the adaptability to a mass spectrum imaging technology is higher; according to the method, 6 times of linear expansion of the hydrogel is successfully realized, and spatial distribution characterization of lipid in liver lobules is realized under the spatial resolution of 100 microns. The method is low in cost and simple and convenient to operate, and has a wide application prospect in popularization of a high-resolution mass spectrum imaging technology at a biological tissue molecule level.

The invention discloses a dynamic test method and system for tissue frequency domain impedance characteristics, and belongs to the technical field of medical detection. The system comprises an energy generator, a bipolar scalpel, a signal generator, a radio frequency power amplifier module, a control unit, two loop switches, a sampling probe and a test unit. An electrode of the bipolar scalpel is used for being in contact with a biological tissue to be subjected to frequency domain impedance characteristic testing; the energy generator is connected with the first loop switch and is used for outputting an electric resection / electrocoagulation signal to the biological tissue so as to dynamically reproduce the state change of the biological tissue; and the signal generator inputs a broadband excitation voltage signal to the biological tissue through the radio frequency power amplifier module and the second loop switch. The control unit is used for coordinating on-off of the two loop switches. The sampling probe is used for collecting voltage and current signals of the double electrodes in real time and feeding back the signals to the testing unit. The test unit generates a real-time impedance characteristic spectrogram of the biological tissue along with frequency change. The tissue impedance change can be monitored on line in real time.

The invention discloses a tissue broadband domain impedance characteristic test system and method, and belongs to the technical field of medical detection. The system comprises a signal acquisition and control unit, a radio frequency power amplifier module, a protective resistor, a bipolar scalpel, two voltage probes and a sampling resistor module, an electrode of the bipolar scalpel is in contact with a biological tissue to be subjected to impedance characteristic test; the signal acquisition and control unit outputs a broadband excitation voltage signal, the broadband excitation voltage signal is amplified by the radio frequency power amplifier module, and the amplified signal sequentially passes through the protective resistor, the sampling resistor module and the electrode to be input into biological tissue; the two voltage probes are respectively used for sampling voltage signals of the electrode and the sampling resistor module in real time and feeding back the voltage signals to the signal acquisition and control unit; and the signal acquisition and control unit obtains impedance amplitudes and impedance phases of the biological tissue under various frequencies through Fourier transform, and impedance characteristic testing is completed. Data support can be provided for the design of the energy surgical instrument.

The invention provides a guiding device, an implantation system and a guiding method for a flexible electrode. The guiding device comprises a guiding needle, the guiding needle comprises a needle body and a needle tip located at the front end of the needle body, a concave part comprising a first side surface and a second side surface which are opposite is arranged at the position, close to the front end of the needle body, of the needle body, and the first side surface is closer to the front end of the needle body; the second side surface is provided with a protruding part which extends towards the first side surface and can penetrate through the through hole of the flexible electrode. And the needle sleeve is provided with a hollow structure to provide a channel in the needle sleeve so as to allow the guide needle to penetrate through the channel to move, a first opening is formed in the front end of the needle sleeve so as to allow the joint part of the flexible electrode to slide into the concave part of the guide needle through the first opening, and the convex part of the guide needle can penetrate through the through hole of the flexible electrode so as to carry the flexible electrode to move. According to the invention, the combination and separation of the guide needle and the flexible electrode can be conveniently realized through the cooperation of the guide needle and the needle sleeve, and the damage probability to the flexible electrode and the mechanical disturbance to biological tissues are reduced.

The present disclosure relates to a surgical system comprising: a control circuit; and an output circuit coupled to the control circuit and configured to deliver energy to an output terminal for delivery to a patient, the output terminal configured to be coupled to an electrosurgical apparatus having two jaws with corresponding electrodes, wherein the control circuit is configured to: deliver a first therapeutic electrosurgical energy pulse to biological tissue in electrical communication with the two electrodes of the electrosurgical apparatus, wherein the first therapeutic electrosurgical energy pulse is followed by a first dwell time; determine an amount of energy delivered to the jaws during the first therapeutic electrosurgical energy pulse; calculate a second dwell time based on the determined amount of energy delivered during the first therapeutic electrosurgical energy pulse, wherein the second dwell time is less than the first dwell time; and deliver a second therapeutic electrosurgical energy to the biological tissue.

Embodiments of the present invention include methods of targeting acidosis (low pH) within the tumor microenvironment (TME) through the use of cathodic electrochemical reactions (CER). Low pH is oncogenic by supporting immunosuppression. Electrochemical reactions create local pH effects when a current passes through an electrolytic substrate such as biological tissue. Electrolysis has been used with electroporation (destabilization of the lipid bilayer via an applied electric potential) to increase cell death areas. However, the regulated increase of pH through only the cathode electrode has been ignored as a possible method to alleviate TME acidosis, which could provide substantial immunotherapeutic benefits. Here, ex vivo modeling shows that CERs can intentionally elevate pH to an anti-tumor level and that increased alkalinity promotes activation of naïve macrophages. Embodiments of the invention include pairing CER treatment protocols with existing electric field-based cancer therapies or use as a stand-alone therapy.

The invention relates to a biomedical image feature fusion method based on a multi-scale heterogeneous hypergraph. The method comprises the following steps: S1, constructing a cell-level hypergraph model; s2, constructing a region-level hypergraph model; s3, constructing a sample-level hypergraph model; s4, transmission and fusion of cross-level hypergraph features: through a level graph neural network HGCN and a dynamic attention mechanism, transmission of cell-level hypergraph features-region-level hypergraph features-sample-level hypergraph features is carried out, global alignment is carried out, and global hypergraph features are obtained; and S5, constructing a histomorphological classification model by using the global hypergraph features, and outputting sample feature representation. According to the method, through multi-scale heterogeneous hypergraph modeling and cross-level dynamic feature fusion, the problems of single-scale characterization limitation and heterogeneous data high-order interaction bottleneck are solved, the cross-scale characterization capability of the biological tissue microenvironment is remarkably improved, the adaptability, generalization performance and analysis precision of a classification model to complex data scenes are enhanced, and the method is suitable for being popularized and applied. And a general framework is provided for complex biomedical analysis tasks.

The invention relates to a sample pretreatment method for desorption electrospray ionization mass spectrometry imaging of a biological tissue frozen section, and the method comprises the following steps: pre-spraying the surface of the frozen biological tissue section, a pre-spraying solvent being a histocompatible solvent, and being used for enhancing the extraction of components in biological tissue cells, the detection sensitivity of components in biological tissues, especially in cells, is improved.

The invention relates to a biological tissue full-waveform inversion ultrasonic tomography method which comprises the following steps: placing ultrasonic transducers in a pure water medium with known sound velocity, and recording ultrasonic signal data of each transducer array element; setting a virtual source array in each transducer array element, constructing an optimization problem of sound source directivity and receiver response, and performing iterative optimization to obtain a final response coefficient and a virtual source signal of each transducer array element; the method comprises the following steps: fixing a biological tissue to be detected in an ultrasonic transducer, and collecting experimental observation signals received by each transducer array element; setting a sound velocity model to carry out forward simulation, taking the obtained virtual source signal as a sound source output, interacting with the sound velocity model, and multiplying by a response coefficient to obtain a forward simulation signal; and performing iterative updating on the sound velocity model according to the experimental observation signal and the forward simulation signal to obtain an ultrasonic tomography result. Compared with the prior art, the method has good flexibility and anti-noise capability, and the quality of the reconstructed image is remarkably improved.

The first aspect of this invention provides a polarized illumination system for providing illumination light to biological tissue, comprising at least one laser, a lens, and a polarization-maintaining fiber. The second aspect provides a polarized imaging system for receiving light reflected or scattered by biological tissue after illumination, comprising an analyzer, an imaging lens, and a photosensitive device. The third aspect provides another polarized imaging system, comprising an imaging lens, a polarizing beam splitter, and a photosensitive device. The fourth aspect provides an endoscope system comprising the aforementioned polarized illumination system and polarized imaging system. This invention utilizes the different depolarization characteristics of scattered light at different depths to achieve separate imaging of specular reflection light, surface scattered light, and deep scattered light, greatly improving the visibility of mucosal surface and deep mucosal information, and significantly enhancing the visibility of various mucosal lesions.

An ultrasonic elasticity measurement device (100) includes an ultrasonic probe (101), which includes a vibrator (1012) and a transducer (1011). The transducer includes multiple array elements. The ultrasonic elasticity measurement device also includes a vibration control module (103), a transmit / receive controller (104), and a data processor (107). The vibration control module generates a vibration sequence and outputs it to the vibrator in a mode that simultaneously performs strain detection and vibration elasticity detection. The ultrasonic probe vibrates under the drive of the vibrator, emitting ultrasonic waves to biological tissue or receiving ultrasonic echoes to obtain ultrasonic echoes for strain detection and ultrasonic echoes for vibration elasticity detection. The data processor calculates strain-type elasticity results based on the ultrasonic echoes of strain detection and calculates vibration elasticity results based on the ultrasonic echoes of vibration elasticity detection, thereby realizing simultaneous strain elasticity imaging measurement and vibration elasticity measurement.

The invention discloses a preparation method of engineered bacteria for releasing CO gas under initiation of ultrasonic waves to kill tumor cells, and relates to a preparation method of CO carrier engineered bacteria. The invention aims to solve the problems that the existing CO carrier material is low in delivery efficiency, the CO is difficult to release efficiently and controllably in space and time at the same time, and the existing metal CO carrier material is high in toxicity. Meanwhile, the problems that the penetrating power of light waves to biological tissues is limited, and the CO release efficiency is limited due to a tumor hypoxic microenvironment are solved. The preparation method comprises the following steps: 1, modifying the surface of escherichia coli with 3-hydroxyflavone-D-alanine; and 2, continuously modifying the surface of the escherichia coli with CaO2 (at) polymyxin B. The method is used for preparing the engineered bacteria for releasing the CO gas through ultrasonic initiation to kill the tumor cells.

The application discloses a terahertz biological tissue imaging system and method based on a metasurface antenna. The terahertz biological tissue imaging system comprises a terahertz signal generator, an intelligent system control device, a terahertz signal transmitter and a data acquisition module. The terahertz signal generator is used for generating an ultra-wideband terahertz wave signal. The intelligent system control device controls the terahertz signal generator to continuously generate the ultra-wideband terahertz wave signal. The terahertz signal transmitter is used for transmitting the ultra-wideband terahertz wave signal after programmable modulation to a biological tissue organ. The terahertz signal receiver is used for receiving terahertz biological signals scattered inside and around the biological tissue organ, and modulating the received terahertz biological signals. The data acquisition module is used for acquiring the terahertz biological signals. The intelligent sensing signal processing module is used for processing the terahertz biological signals to obtain a terahertz image of the biological tissue organ. The application solves the problems of narrow bandwidth and low gain in the corresponding technology.

A device for interfacing at least one filamentous structure, with real or simulated biological tissue, comprising at least one body (10, 20) for anchoring the filamentous structure, said device being characterized in that said body (10, 20) comprises: at least one capstan (12, 22, 22', 22'), said capstan (12, 22, 22') being configured for wrapping the filamentous structure; at least one porous portion (13, 13', 24) having a trabecular structure (300); and system for regeneration, or repair, or replacement, or simulation of tendon and / or ligamentous tissue comprising said device and at least one filamentous structure comprising a plurality of nanofiber assemblies obtained by electrospinning, said plurality of assemblies being arranged to form a single bundle; said bundle being wrapped to the capstan (12).

This invention provides a feeding mechanism for a biological tissue sample staining device, including a translational feeding assembly, a sample slide feeding lifting assembly, a sample slide feeding heating chamber, and a microscope slide vertical support. The translational feeding assembly has a translational feeding platform capable of holding multiple microscope slide vertical supports. Multiple microscope slides can be vertically fixed in an array within each microscope slide vertical support, with each slide spaced apart. The sample slide feeding lifting assembly cooperates with the translational feeding platform. The sample slide feeding heating chamber is positioned above the sample slide feeding lifting assembly, and the sample slide feeding lifting assembly can reciprocate between the translational feeding platform and the sample slide feeding heating chamber. The beneficial effect of this invention is that it can avoid the risk of cross-contamination between microscope slides.

The invention discloses a rapid degreasing method for a biological tissue sample, which comprises the following steps: sequentially carrying out fixing, tissue dehydration, azeotropic degreasing, tissue rehydration and other treatments on the sample, and the degreasing solution is composed of alcohols and hydrocarbons according to a mass ratio of 1: 1-1: 2. According to the rapid degreasing method provided by the invention, the lipid in the tissue is efficiently extracted through mixed azeotropy of the alcohol and hydrocarbon organic reagents, so that the tissue is transparent and transparent; the problem of poor degreasing in tissues is solved by utilizing the high permeability characteristic of the micromolecular organic reagent; the dissolving capacity to phospholipid and cholesterol is improved by mixing organic reagents; through azeotropy, the degreasing efficiency is improved, the degreasing time is shortened, the internal permeability effect of the tissue is improved, and internal and external uniform permeability of the tissue is realized; by adjusting the components of the organic reagent, boiling rapid degreasing at low temperature is realized, and the retention of a fluorescence signal is improved; the tissue treated by the method is transparent and small in deformation, a fluorescent protein signal can be maintained, and the immunofluorescence staining effect can be improved.

The utility model relates to the technical field of cell culture devices, in particular to a biological tissue chopper which comprises a main machine, a cutter assembly and a chopping box, the cutter assembly is connected to the main machine and the chopping box, the cutter assembly is connected to the chopping box, the cutter assembly and the chopping box are connected in a sealed mode, and the chopping box is connected to the main machine. The main machine is used for providing power for working of the cutter assembly and controlling the cutter assembly to conduct up-down vibration chopping work. The biological tissue cutting device solves the technical problems that in the prior art, the biological tissue cutting efficiency is low, and sterile operation is difficult to guarantee, the biological tissue cutting effect is improved, and the sterility and reliability in the biological tissue cutting process are guaranteed.

The invention provides an end-to-end real-time analysis method for an endoscope image fluorescence brightness area based on double deep learning model joint training and a self-group iteration upgrading device. The end-to-end real-time analysis method comprises the following steps: acquiring an image of a biological tissue and processing the image by using a deep learning algorithm to obtain a training data set; sequentially constructing and training an image preprocessing deep learning model and an unsupervised image segmentation deep learning model based on the training data set; inputting image data acquired by the endoscope into an image preprocessing deep learning model to obtain a first output image; inputting the first output image into an unsupervised image segmentation deep learning model to obtain a second output fluorescence image; and carrying out brightness analysis on the second output fluorescence image, and visually outputting and storing the second output fluorescence image. The real-time performance and the accuracy of fluorescence region analysis can be improved. According to the self-group iteration upgrading device, model training iteration upgrading can be carried out in multiple modes, and the performance of the device is continuously improved.

This application belongs to the field of fluorescence microscopy imaging, and more specifically, relates to a dual-gel system suitable for dilatational microscopy of large-volume biological tissues. This dual-gel system achieves uniform expansion and morphological stability of tissue samples through a dilatational gel, and then imparts high mechanical strength using a stabilizing gel. This allows millimeter-scale large-volume biological tissue samples to possess both high expansion ratio and excellent mechanical stability, achieving 3-5 times linear expansion with a Young's modulus of not less than 300 kPa. It can stably adapt to vibrational cutting operations and support cutting of 100-500 μm thick layers, with intact cut surfaces and good interlayer consistency, greatly improving the efficiency and quality of three-dimensional data acquisition. Furthermore, the hydrogel sections obtained from the cutting have intact morphology and can be used as independent slides for multidimensional analysis. This application overcomes the limitations of traditional dilatational microscopy techniques in terms of sample volume and mechanical stability, providing an efficient and reliable solution for the three-dimensional reconstruction of millimeter-scale biological tissues.

This invention relates to the field of medical ablation technology, and discloses a method and system for controlling the temperature of an ablation electrode. The method includes: during the ablation process, acquiring in real time the output power of the ablation host and the biological tissue impedance between the ablation electrode and the lesion tissue monitored by the ablation host; calculating the real-time heat generation power of the ablation electrode acting on the lesion tissue; acquiring the inlet temperature, outlet temperature, and instantaneous flow rate of the cooling medium flowing through the internal cooling channel of the ablation electrode, and quantifying the real-time heat dissipation power of the cooling medium according to the heat transfer relationship of the medium; determining thermal state parameters based on the real-time difference between the real-time heat generation power and the real-time heat dissipation power, the thermal state parameters reflecting the heat accumulation trend; dynamically adjusting the flow rate of the cooling medium using a feedforward method; and in the feedforward dynamic adjustment, collecting and associating successful ablation parameter combinations of biological tissue to construct a self-learning parameter library for the ablation electrode. This invention can improve the accuracy of ablation electrode temperature control.

The invention discloses an all-fiber three-dimensional tomography system, which is used for performing three-dimensional tomography on an object to be observed at a high speed and comprises a light source, a first optical coupler, a first optical circulator, a first optical collimator, a first convex lens, an electric control attenuator, a second optical circulator, a second optical collimator, a second convex lens, a second optical coupler and a balance detector. The light source included in the all-fiber three-dimensional tomography system can quickly perform line or plane scanning on optical scattering media such as biological tissues and the like by utilizing a technology of capturing optical signals to obtain images and adopting an all-fiber line architecture, so as to obtain high-resolution three-dimensional images.

The present invention relates to recellularization of xenogeneic tissue. Specifically, the present invention relates to recellularization of xenogeneic tissue, which enables transplantation of non-immunogenic biological tissue with improved durability and regenerative capacity by generating xenogeneic tissue having regenerative capacity through human cell recellularization after removal of galactose-alpha-1,3-galactose (α-Gal) xenoantigens and non-galactose (non-Gal) xenoantigens.