The invention discloses
pancreatic lipase immobilized composite nano magnetic beads, a preparation method and application, ferroferric
oxide is adopted as a
magnetic core,
dopamine-mediated growth is performed to form a core-shell structure, then the periphery of the core-shell structure is wrapped with a
metal organic framework to form the composite nano magnetic beads, and
pancreatic lipase is immobilized by using the composite nano magnetic beads as an adsorption carrier. The
enzyme loading amount, the repeated
utilization rate and the storage stability of the ferroferric
oxide nano-particles are remarkably improved, the
enzyme immobilization efficiency and the
enzyme activity are improved, and meanwhile, the prepared
pancreatic lipase immobilized composite nano-magnetic beads have excellent stability and
biocompatibility, are green and
pollution-free and can be repeatedly used; the composite nano magnetic beads are synthesized by adopting a solvothermal method, the product is easy to separate, the synthesis process is simple, the enzyme immobilization efficiency can be regulated and controlled by regulating and controlling the synthesis process, the structure of an active ligand can be screened and analyzed from natural products with numerous and jumbled components, and the active ligand has a certain lipid-lowering potential and can be used for preparing the nano magnetic beads. The compound can be used as a potential
lead compound of lipid-lowering drugs.