The invention relates to a perpendicular magnetic anisotropic multi-layered film, which comprises a substrate, a bottom layer, a lower magnetic layer, a middle layer, an upper magnetic layer and a covering layer, wherein at least one of the lower magnetic layer and the upper magnetic layer is a composite magnetic layer which consists of a main layer and a transitional layer, the main layer is made of perpendicular magnetic anisotropic material, and the transitional layer is made of magnetic metal material with spin polarization higher than the spin polarization of the perpendicular magnetic anisotropic material, and is positioned between the main layer and the middle layer. When the middle layer is a barrier layer, the composite magnetic layer also can be made of metal material with a spin diffusion length larger than 3nm. Under the premise of guaranteeing excellent perpendicular magnetic anisotropy, the invention can enhance the magnetoresistance property, reduce mutual magnetostatic reaction and decrease the reverse field or reverse current of a corresponding device. The invention is applicable to giant magnetoresistance devices or tunneling magnetoresistance devices, such as magnetic sensors, magnetic random-access memorys and magnetic logic devices.