235 results about "Carotenoid" patented technology

The invention discloses an apple anthracnose leaf blight recognition five-dimensional data fusion method based on a patrol robot, relates to the technical field of anthracnose leaf blight recognition, and solves the problems of limited sensing dimension, large illumination interference and inaccurate spatial positioning in the prior art. Comprising the following steps: S1, acquiring surface curvature characteristics of apple leaves, collecting RGB-D images of the leaves, constructing a three-dimensional topological structure of the leaves, and positioning scab space distribution; s2, acquiring spectral reflection information of apple leaves, quantifying biochemical components of chlorophyll a and carotenoid, and constructing a vegetation index feature set sensitive to diseases; s3, apple tree canopy temperature information is collected, a transpiration anomaly detection model is constructed, and multi-modal fusion of thermal infrared and point cloud data is realized; and S4, integrating spatial distribution, spectral reflection and thermal radiation information, constructing a five-dimensional phenotypic characteristic matrix model, and comprehensively analyzing apple phenotypic characteristics. According to the method, the recognition accuracy under the conditions of blade shielding, uneven illumination, blade posture change and environment interference can be remarkably improved.

Provided herein are pharmaceutical compositions comprising carotenoids, including liposomes that encapsulate carotenoids including ionizable carotenoids such as trans-crocetin. The provided compositions have uses in treating diseases, disorders and conditions associated with, but not limited to, infection, endotoxemia, inflammation, sepsis, ischemia, hypoxia, shock, stroke, lung injury, wound healing, traumatic injury, reperfusion injury, cardiovascular disease, kidney disease, liver disease, inflammatory disease, metabolic disease, pulmonary disorders, blood related disorders and hyperproliferative diseases such as cancer. Methods of making, delivering, and using the pharmaceutical compositions are also provided.

The invention relates to the technical field of carotenoid cleavage dioxygenase, and discloses a carotenoid cleavage dioxygenase mutant and application thereof. In order to improve the efficiency of converting carotenoid in tobacco into aroma molecule beta-ionone, specific mutation of characteristic sites is carried out on carotenoid cleavage dioxygenase (PhCCD1) from petunia, that is, serine (S) at the 428 site of the carotenoid cleavage dioxygenase is mutated into cysteine (C) or tyrosine (Y) to obtain a mutant S428C or S428Y, and the mutant S428C or S428Y is converted into beta-ionone. The yield of the beta-ionone converted from the carotenoid is greatly improved. Experiments prove that the yields of beta-ionone of S428C and S428Y respectively reach 54.2 mg / L and 50.6 mg / L, which are respectively increased by 69.7% and 58.5% compared with those of a wild strain WT.

The invention discloses a plant leaf physicochemical parameter inversion method based on spectrum two-dimensional characterization and multi-task deep learning, and belongs to the technical field of plant physiological detection. The method comprises the following steps: constructing a hyperspectral plant leaf data set; preprocessing a hyperspectral plant leaf data set; reconstructing the preprocessed one-dimensional spectral vector into a two-dimensional image; taking the generated two-dimensional image as input, constructing a multi-task learning model, and performing training and evaluation; and applying the trained model to a test set to obtain an inversion result of the physical and chemical parameters of the leaf. The method solves the problem of parameter inversion interference caused by the same-spectrum foreign matter phenomenon, achieves the precise modeling of local fine feature extraction and global context relation, solves the inversion precision bottleneck caused by spectral response overlapping among parameters, improves the distinguishing capability of the model for easily confused parameters such as chlorophyll and carotenoid, and improves the accuracy of the model. And finally, high-precision and robust synchronous inversion of the physical and chemical parameters of the plant leaves is realized.

The invention relates to the technical field of scenic spot plant growth monitoring, and particularly discloses a scenic spot plant growth intelligent monitoring method and system, and the method comprises the steps: carrying out the multi-band image data collection of ornamental vegetation, and obtaining leaf spectrum purification data; extracting wave band response curves of anthocyanin, carotenoid and chlorophyll according to the spectral purification data to obtain pigment wave band characteristic data; performing spectrum aliasing sensitivity analysis on the pigment wave band characteristic data to obtain spectrum aliasing nonlinear characteristic data; carrying out differentiation attribution calculation on a wave band aliasing region between the healthy purple leaves and the iron-deficient yellowing to obtain aliasing difference attribution data; and finally obtaining vegetation pigment unmixing mapping data. Performing physiological status difference identification on the pigment unmixing mapping data to obtain a vegetation health monitoring result; the precision, high efficiency and foresight of scenic spot vegetation monitoring are technically guaranteed, the technical requirements of modern scenic spot vegetation management are met, and losses caused by vegetation health problems are effectively reduced.

The invention provides application of riboflavin in inhibition of postharvest yellowing of broccoli, discovers that yellowing and aging of broccoli can be effectively inhibited by soaking broccoli with a riboflavin-containing solution, and the mechanism of riboflavin in inhibition of broccoli yellowing is deeply studied. The riboflavin is found to be capable of inhibiting expression of chlorophyll degradation genes and chlorophyll aging genes in the broccoli and delaying synthesis of carotenoids by the broccoli, so that yellowing and aging of the broccoli are effectively inhibited. The broccoli treated by the method disclosed by the invention has an excellent treatment effect, and yellowing and aging of the broccoli can be obviously delayed and the shelf life can be prolonged when the broccoli is stored at normal temperature, so that the method has great significance in storage of the broccoli and is also greatly helpful for production and sales of the broccoli.

The invention discloses application of an SlBRL2 gene in improvement of tomato fruit quality, and belongs to the technical field of plant genetic engineering. According to the invention, the gene SlBRL2 related to the tomato fruit quality is found, and the CDS sequence of the gene SlBRL2 is as shown in SEQ ID NO. 1. It is found for the first time that the knockout of the SlBRL2 gene can significantly improve the tomato fruit shape index, the fruit hardness, the soluble solid content, the total sugar content and the carotenoid content, the taste and the nutritional quality of tomatoes are improved while the storage resistance and the transportation resistance of the tomatoes are improved, and a new direction is provided for tomato germplasm breeding.

The invention provides an edible coating fresh-keeping liquid suitable for low-temperature refrigeration of harvested tropical fruits and a storage and fresh-keeping method. The low-temperature cold storage edible film coating fresh-keeping liquid is soybean oil. When bananas or mangoes are coated with the edible coating liquid for low-temperature refrigeration, the occurrence of fruits under low-temperature refrigeration can be effectively inhibited, and the sensory quality and shelf life of the fruits can be maintained. Bananas or mangoes are subjected to soybean oil coating treatment, so that the relative conductivity of fruit tissues can be effectively reduced, and the integrity of fruit cell membranes is maintained; according to the present invention, the production of H2O2, O2 <-> and MDA can be significantly reduced, the activity of POD can be inhibited, and the activity of PPO, SOD and CAT can be enhanced so as to activate the anti-oxidation defense system of fruits, reduce the oxidative damage caused by cold injury, and enhance the accumulation of osmotic regulation substances; the content of antioxidant carotenoid can be increased, so that the stress resistance of the fruits is improved, and the cold injury of the fruits in the low-temperature storage process is reduced.

To develop a molecular marker related to carotenoid accumulation for the Patinopecten yessoensis variety "Haidu Jinbei" and to provide a method for maintaining population quality.SOLUTION: An InDel molecular marker related to carotenoid accumulation of Patinopecten yessoensis and an application thereof are disclosed. A base sequence of the InDel molecular marker in carotenoid accumulating Patinopecten yessoensis and a base sequence of the InDel molecular marker in common Patinopecten yessoensis are disclosed. Furthermore, an upstream primer sequence and a downstream primer sequence of a primer pair for amplifying the InDel molecular marker related to carotenoid accumulation of Patinopecten yessoensis are disclosed. By detecting the InDel molecular marker, homozygous Patinopecten yessoensis individuals capable of accumulating carotenoids can be conveniently and rapidly screened in a seedling stage and from parents, and effective technical support is provided for breeding of "Haidu Jinbei" and optimization of characters.SELECTED DRAWING: Figure 2

The present disclosure relates to compositions and methods for producing carotenoids. The present disclosure provides genetically modified cells (e.g., microbes) that comprise a transgene encoding a ferredoxin protein, and methods of making and using said genetically modified cells. The present disclosure also provides genetically modified cells (e.g., microbes) that comprise a transgene encoding a CBP or a genetic modification capable of increasing the expression of a CBP, and methods of making and using said genetically modified cells.

The invention relates to a feed additive for producing high-nutrition low-cholesterol eggs. The feed additive comprises natural grains, microalgae and a microalgae extract, and is prepared from the following raw materials in percentage by weight: 20-70% of corn, 5-10% of wheat bran, 5-10% of soybean meal, 5-20% of spirulina powder, 5-20% of chlorella powder and 5-20% of haematococcus pluvialis. The feed additive is added into laying fowl feed according to the proportion of 1-2wt%, the feed additive and the laying fowl feed are uniformly mixed and then fed, low-cholesterol poultry eggs rich in protein, algae source carotenoid, calcium, magnesium, zinc, iron and other nutritional ingredients can be stably obtained after the laying fowl feed is fed for 2 weeks, compared with common poultry eggs, the cholesterol content can be reduced by 26%, and the poultry eggs are fine and smooth in taste, free of fishy smell and good in taste. The nutrition and quality of poultry eggs are remarkably improved, and economic benefits are improved.

The application discloses a high-absorption carrot juice processing control method based on multi-stage quality detection, and relates to the technical field of food processing. The method comprises the following steps: raw material acceptance and initial physical property detection; low-temperature color protection pretreatment; dynamic pressing and initial filtration; enzyme solubilization treatment to release embedded carotenoids; three-stage high-pressure homogenization to reduce the D[4,3] particle size to below 380 nanometers; instantaneous high-temperature sterilization to promote interface membrane reconstruction; multi-stage quality online detection is implemented at four key nodes; closed-loop parameter feedback adjustment is realized based on detection data through a multivariate regression model; and finally, sterile cold filling and light-proof aging are performed. Through the technical scheme, the application significantly improves the carotenoid retention rate, oxidation stability and in-vitro gastrointestinal simulation absorption rate, the final product absorption rate is stably above 42%, the retention rate is above 92%, and no organic solvent or non-original juice processing means is needed, so the application is suitable for industrialized production of high-value-added pure carrot juice.

Based on co-drugs representing two different chemical entities and the co- and sequential administration of the two chemical entities, novel therapies for macular degeneration and TTR amyloidosis are provided. The first component (“selective TTR ligand”) is a chemical entity that binds to TTR in the RBP4 (retinol-binding protein 4)-TTR (transthyretin) complex, which participates in the delivery of retinol to the retina. This component reduces retinol transport from circulation to the retina and provides stabilization of the TTR tetramer. The second component (“C20-D3-visual chromophore-generating compound”) is a C20-D3 modified retinoid or carotenoid that, when metabolized in mammals, ultimately produces a C20-D3 visual chromophore, which is presented in the retina as C20-D3-9-cis-retinal or C20-D3-11-cis-retinal. Deuteration at C20 reduces the formation of lipofuscin biretinol, but other functions (such as providing a precursor for the synthesis of the visual chromophore 11-cis-retinaldehyde in vivo) are not reduced.

To provide a urine inspection method which allows detection of a carotenoid concentration in urine in a simple manner.SOLUTION: Provided is a urine inspection method for detecting a carotenoid concentration in urine, by using a urine specimen to which an alkaline additive agent has been added.SELECTED DRAWING: Figure 1

The invention discloses application of a lycium barbarum Lb03G03224 gene in catalysis of esterification of free carotenoids, the nucleotide sequence of the lycium barbarum Lb03G03224 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of encoded protein is as shown in SEQ ID NO: 2. According to the present invention, it is found that the zeaxanthin dipalmitate and the isomer 9 (Z)-zeaxanthin dipalmitate thereof can be generated when the Lb03G03224 gene is expressed in the engineering escherichia coli for generating beta-carotene, beta-cryptoxanthin and zeaxanthin, such that the zeaxanthin dipalmitate and the isomer 9 (Z)-zeaxanthin dipalmitate can be produced, the protein coded by the Lb03G03224 has the function of catalyzing esterification reaction of carotenoid containing free hydroxyl and fatty acid acyl donor, and has important application value in in-vitro biosynthesis of esterified carotenoid.

The invention discloses a dCAPS molecular marker for identifying sweet potato pulp color characters and application of the dCAPS molecular marker, and belongs to the technical field of sweet potato breeding. According to the dCAPS molecular marker provided by the invention, on the basis of SNP variation sites, mismatched bases are introduced to form restriction endonuclease restriction enzyme cutting sites, the sweet potato pulp color can be judged by utilizing PCR-restriction enzyme cutting-polyacrylamide gel electrophoretic separation, and then sweet potato varieties with high and low carotenoid contents can be identified according to the sweet potato pulp color. The method has important guiding significance and application value for assisting sweet potato carotenoid content related molecular breeding research.

This invention discloses a method for enhancing the color and aroma of whole-component fruit juice through synergistic fermentation with lactic acid bacteria, belonging to the field of food processing technology. The method uses ripe mangoes / pineapples as raw materials. After washing, pitting (peeling), and low-temperature jet milling to retain all components, the juice undergoes compound enzymatic hydrolysis and optimization of its uniformity. A compound strain composed of *Lactobacillus plantarum*, *Lactobacillus fermentum*, and *Lactobacillus helveticus* is then inoculated. The mixture undergoes constant-temperature anaerobic fermentation. The process is optimized using fuzzy mathematical sensory evaluation and response surface methodology, resulting in a sensory score of 91.67 for mango juice and 90.83 for pineapple juice. The retention rate of carotenoids is increased by 25%-30%, the lactic acid content reaches 2.97-26.45 mg / g, and the relative content of esters in volatile aroma components increases to over 35%. Simultaneously, the total sugar content decreases by 3.7%-24.0%. This method solves the problems of dull color, weak aroma, and nutrient loss in traditional whole-component fruit juices. The product combines natural functionality with a unique fermented flavor, making it suitable for industrial production.

The invention relates to the technical field of genetic engineering, and discloses a genetically engineered bacterium for producing beta-ionone at high yield and application of the genetically engineered bacterium. A synthetic biological plasmid modularization technology is adopted, coding genes of carotenoid cleavage dioxygenase NtCCD1 in tobacco are excavated, and specific soluble protein tags including a thioredoxin tag, a maltose binding protein tag and a glutathione transferase tag are screened, so that the content of the carotenoid cleavage dioxygenase NtCCD1 in the tobacco is determined. The NtCCD1 is subjected to single-point mutation modification of mutation of 431st-site histidine into tyrosine, lysine or glutamine, and the fermentation enzyme and the gene engineering bacterium which can efficiently catalyze splitting decomposition of beta-carotene in tobacco to produce beta-ionone are constructed. The biosynthesis of the tobacco aroma component beta-ionone is realized, and the method has high efficiency.

The invention discloses seed pumpkin magnetic biochar as well as a preparation method and application thereof, and belongs to the technical field of biochar materials. The method comprises the following steps: removing seeds from seed pumpkins, cleaning, slicing, drying, crushing and sieving to obtain seed pumpkin powder with the particle size of less than 212 microns and seed pumpkin residues 1 with the particle size of more than 212 microns; carrying out ultrasonic extraction on the obtained seed pumpkin powder by adopting a deep eutectic solvent, and carrying out solid-liquid separation to obtain a solid, namely seed pumpkin residues 2; mixing the seed pumpkin residue 1 with the obtained seed pumpkin residue 2, and drying; mixing and stirring the obtained solid with an iron salt solution, standing, carrying out solid-liquid separation, taking a solid phase, and drying to obtain pumpkin residue powder; under a protective atmosphere, pumpkin residue powder is pyrolyzed, washed and dried to obtain the seed pumpkin magnetic biochar (PMB). The PMB and carotenoid in the pumpkin seed oil have relatively strong interaction, and the PMB has relatively good reutilization efficiency and can be effectively applied to adsorption of pigments in crude oil of the pumpkin seed oil.

The present application relates to the technical field of synthetic biology, in particular to a multi-module synergistic optimization of brown cyanidin and brevifolin production of saccharomyces cerevisiae engineering bacteria and its construction method and application. The saccharomyces cerevisiae engineering bacteria includes naringenin synthesis pathway, optimization pathway of shikimic acid, synergistic pathway of phenylalanine, 6-OH luteolin synthesis pathway, brown cyanidin and brevifolin synthesis pathway, multiple copies of SbF6H and MpalOMT2, NADPH optimization pathway, and SAM cycle optimization pathway. The saccharomyces cerevisiae engineering bacteria is used for producing brown cyanidin and brevifolin, and improving the yield of the two.

The invention relates to the technical field of biological medicines, in particular to application of canthaxanthin extract and a feed additive containing the canthaxanthin extract in silkworm breeding, and finds that cocoon shell amount, whole cocoon amount and pupa weight of silkworms can be remarkably increased and egg laying amount of single moths can be increased by adding canthaxanthin extract to silkworms for the first time. Meanwhile, the silkworm variety with functional carotenoid binding protein can also be induced to produce orange red natural colored cocoons. The invention further provides a compound feed additive containing the canthaxanthin extract and the sericin-derived small peptide and / or gamma-aminobutyric acid. Experiments prove that the canthaxanthin extract and the sericin-derived small peptide have a synergistic effect in the aspect of increasing the cocoon shell amount. According to the technical scheme, on the premise that normal growth and development of silkworms are not affected, the multiple purposes of yield increasing, propagation promoting and color increasing are achieved at the same time, and the method has good application prospects and industrial value.

The application discloses a cucumber CsCCD1 gene and application thereof in regulating fruit flesh color of cucumber. A CsCCD1 gene is cloned from cucumber, and it is first disclosed that the cucumber CsCCD1 gene has the function of regulating fruit flesh color. The coding region of the CsCCD1 gene is edited by using a CRISPR-Cas9 technology, and it is found that after the CsCCD1 gene is mutated, the fruit flesh color of the cucumber is significantly yellow, and the carotenoid content of the fruit is significantly improved. The application not only lays an important foundation for revealing the genetic and biological mechanism of the fruit flesh color of the cucumber, but also provides a new gene resource for directional improvement and cultivation of new varieties with high quality of the cucumber, lays a theoretical foundation for the later molecular breeding work, and has important application value.

The invention relates to the field of tobacco, in particular to an expression cassette, an expression vector and a preparation for improving tobacco quality and application thereof. The present invention provides an expression cassette comprising: a gene encoding carotenoid cleavage dioxygenase and a promoter. Probiotic escherichia coli E. coli Nissle 1917 is used as an expression host, genetically engineered bacteria for heterologously expressing carotenoid cleavage dioxygenase are constructed, endotoxin is prevented from being introduced in the tobacco leaf fermentation process, and a biological preparation capable of increasing aroma substances of tobacco leaves can be obtained through simple cell disruption. Meanwhile, the content of aroma substances such as geranyl acetone and ionone in the tobacco leaves can be remarkably increased through tobacco leaf fermentation.

The invention discloses a photoisomerization method of carotenoid, which comprises the following steps: dispersing high cis carotenoid in an organic solvent, carrying out photoisomerization in an illumination kettle until the proportion of all-trans carotenoid is not obviously increased, cooling, crystallizing, and filtering to obtain carotenoid solid with high all-trans proportion, and the filtrate sleeve is used as a solvent for next batch of isomerization. The isomerization method has the characteristics of mild conditions, high efficiency, controllability, cleanness and the like, accords with green chemistry and sustainable development concepts, and is simple in isomerization extraction operation, high in extraction yield and suitable for large-scale implementation.

The invention provides a senescent muscle repairing composition and application, and belongs to the technical field of cosmetics. Comprising polyunsaturated fatty acid, carotenoid, peptides, polydeoxyribonucleotide PDRN and at least one algae extract selected from chlorella, spirulina, brown algae and red algae. According to the senescent muscle repairing composition prepared by the invention, through a carefully selected natural active component system, the composition has a synergistic effect from two major directions of promoting synthesis of ECM key components and inhibiting excessive degradation of ECM, and improves the structural quality of ECM, so that the steady state of skin ECM is effectively recovered, and the purposes of improving wrinkles and improving compactness and elasticity are achieved.

The application discloses a method for regulating body color of ornamental fish and belongs to the technical field of aquaculture. The application finds that, by using thyroid hormone to soak ornamental fish in the fry period and the juvenile fish period, excessive thyroid hormone can significantly reduce the number of red pigment cells and the size of pigment granules of ornamental fish in different periods, and reduce the content of carotenoids; when the synthesis of thyroid hormone is inhibited, the expression of melanin cells and related synthesis pathway genes is promoted, and the carotenoid level is increased. The application first clearly defines the inhibitory effect of thyroid hormone on the development of red pigment cells and the accumulation of carotenoids of gourami, fills the knowledge gap in the field, and provides important guiding information for future regulation of body color and genetic improvement of ornamental fish and other aquatic animals.

The invention is applicable to the technical field of agricultural planting, and provides a universal water-soluble fertilizer for agricultural planting and a preparation method of the universal water-soluble fertilizer. The water-soluble fertilizer comprises the following components in parts by weight: 10-20 parts of monopotassium phosphate, 8-14 parts of monoammonium phosphate, 6-12 parts of calcium nitrate, 5-12 parts of amino acid calcium, 7-16 parts of carotenoid, 3-8 parts of yeast nucleotide, 2-4 parts of glucan, 5-10 parts of a seaweed extract, 9-21 parts of fish meal, 6-14 parts of fish protein, 1.5-3.5 parts of a chelating agent, 0.5-3 parts of a penetrant, 0.4-2 parts of an auxiliary agent and 1-3 parts of trace elements. The water-soluble fertilizer product prepared by the invention not only meets macroelements required by crop growth, but also improves the comprehensive efficiency of the fertilizer by adding bioactive substances such as amino acid calcium, carotenoid, yeast nucleotide and the like; the universal water-soluble fertilizer can flexibly meet the nutritional requirements of different crops in different growth stages and different use environments, and effectively solves the problem that the existing water-soluble fertilizer is difficult to flexibly adjust.

The present application provides a kind of whole fruit fruit wine, it is by fruit, 50 degrees of liquor base liquor, yellow sugar, honey proportion mixed soak and is made into.The fruit is unique plant in Wudang Mountain of Hubei, has the effect of generating fluid to quench thirst, clearing spirit and reducing qi, is respected as holy fruit in ancient times, Ming Dynasty emperor Zhu Di also lists fruit as tribute to the court.Fresh fruit contains rich phosphorus, iron, calcium, vitamin C, carotene and other nutrients and seventeen kinds of amino acids necessary for human body, has the effect of helping digestion, resolving phlegm, tonifying kidney, refreshing brain, invigorating blood, strengthening body, tranquilizing pain, reducing pressure and reducing lipid, also has the health care effect of anti-inflammatory analgesic, eliminating fatigue, detoxification, enhancing immunity, etc.The present application not only improves the utilization value of fruit, but also greatly improves the nutritional and health care function of fruit wine, with low cost, improves economic benefit, and creates good social benefit.

The invention belongs to the technical field of Chinese wolfberry processing, and particularly relates to fresh Chinese wolfberry primary pulp rich in high-value active ingredients and a production method of the fresh Chinese wolfberry primary pulp. The method is characterized in that grinding-pulsed electric field circulation treatment is carried out, so that the retention rate of heat-sensitive active ingredients such as lycium barbarum polysaccharide, carotenoid and vitamins is increased, the flavone content is 0.41-0.45 g / 100 g, the lycium barbarum polysaccharide content is 0.54-0.57 g / 100 g, the beta-carotene content is 0.55-0.60 mg / g, the zeaxanthine content is 1.28-1.35 mg / g, the betaine content is 0.340-0.366 g / 100 g, and the linoleic acid content is 0.052-0.057 g / 100 g. The content of all nutrient elements in the Chinese wolfberry primary pulp in the prior art is far higher, and the nutrition and the efficacy value of the fresh Chinese wolfberry fruits are maximized.