400 results about "Carotenoid" patented technology

The invention belongs to the technical field of hyperspectral imaging and machine learning, and discloses a plant carotenoid rapid determination method based on hyperspectrum and machine learning. The measuring system comprises a hyperspectral imaging device, a spectrum preprocessing module, a characteristic wavelength extraction module, a machine learning modeling module and a carotenoid content prediction module which are sequentially connected through a data processing unit. According to the method, spectral data of a plant sample is obtained in combination with a hyperspectral imaging technology, key wavelengths are screened through spectrum preprocessing and a feature extraction algorithm, and a carotenoid content prediction model is established by using machine learning models such as random forest regression, partial least squares regression or a gradient elevator. According to the method, rapid and nondestructive detection of the plant carotenoid can be realized without a chemical reagent, the determination precision and efficiency are improved, and the defects of complex operation, long time consumption and strong destructiveness of a traditional detection method are overcome.

The invention relates to the technical field of microorganisms, and provides heteromorpha sp. RXH-HY13 and application of the heteromorpha sp. RXH-HY13. The invention provides a dysococcus globosum RXH-HY13, which is preserved in the China Center for Type Culture Collection on July 11, 2024, the preservation number of the dysococcus globosum RXH-HY13 is CCTCC NO: M 20241535, and the preservation number of the dysococcus globosum RXH-HY13 is CCTCC NO: M 20241535. The invention also provides an application of the special-shaped algae RXH-HY13. The special-shaped algae RXH-HY13 is used for enriching at least one of organic selenium and carotenoid in a selenium ion stress environment; the invention also provides another application of the heteromorphic algae RXH-HY13. The application comprises the step that the heteromorphic algae RXH-HY13 is used for preparing a product rich in at least one of organic selenium and carotenoid. The special-shaped algae RXH-HY13 provided by the invention has certain tolerance to a selenium ion stress environment, so that the special-shaped algae RXH-HY13 not only can enrich organic selenium in the selenium ion stress environment, but also can promote accumulation of various carotenoids, and has the effect of synergistically enriching the organic selenium and the various carotenoids.

The invention discloses an apple anthracnose leaf blight recognition five-dimensional data fusion method based on a patrol robot, relates to the technical field of anthracnose leaf blight recognition, and solves the problems of limited sensing dimension, large illumination interference and inaccurate spatial positioning in the prior art. Comprising the following steps: S1, acquiring surface curvature characteristics of apple leaves, collecting RGB-D images of the leaves, constructing a three-dimensional topological structure of the leaves, and positioning scab space distribution; s2, acquiring spectral reflection information of apple leaves, quantifying biochemical components of chlorophyll a and carotenoid, and constructing a vegetation index feature set sensitive to diseases; s3, apple tree canopy temperature information is collected, a transpiration anomaly detection model is constructed, and multi-modal fusion of thermal infrared and point cloud data is realized; and S4, integrating spatial distribution, spectral reflection and thermal radiation information, constructing a five-dimensional phenotypic characteristic matrix model, and comprehensively analyzing apple phenotypic characteristics. According to the method, the recognition accuracy under the conditions of blade shielding, uneven illumination, blade posture change and environment interference can be remarkably improved.

The invention discloses pseudomonas TB392 and application thereof in degradation of pectin and generation of flavor substances in tobacco leaves, and belongs to the technical field of biology. The strain is preserved in the China General Microbiological Culture Collection Center on October 16, 2024, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.32230. The strain can produce pectinase at high yield, has the capability of reducing nicotine, can degrade macromolecular compounds such as carotenoid, starch, pectin, cellulose and the like in tobacco leaves, and can remarkably improve the content of flavor substances in the tobacco leaves. After the strain or the prepared microbial agent is applied to tobacco leaves, the quality of the tobacco leaves can be improved, and the use value of the tobacco leaves is improved.

The invention discloses an EMS-ARTP compound mutation chlorella pyrenoidosa mutant strain and an application thereof. The preparation method comprises the following steps: mutagenizing a chlorella pyrenoidosa mutant strain CX41 by adopting an ethyl methanesulfonate (EMS) and normal-pressure and room-temperature plasma (ARTP) composite technology, screening and verifying to obtain a golden yellow chlorella pyrenoidosa mutant strain, naming the golden yellow chlorella pyrenoidosa mutant strain as chlorella pyrenoidosa Y11, and screening and verifying the golden yellow chlorella pyrenoidosa Y11 to obtain the chlorella pyrenoidosa Y11. The strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on the fifth floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou on October 23, 2024, and the preservation number is GDMCC No: 65324. The chlorella pyrenoidosa mutant strain Y11 has the characteristics of high yield of protein and rich carotenoids, and can be used for fermentation production of microalgae protein and carotenoids.

The invention discloses a chlorella pyrenoidosa white mutant strain FZU65-W1 and application of the chlorella pyrenoidosa white mutant The classification name of the chlorella pyrenoidosa white mutant strain FZU65-W1 is chlorella pyrenoidosa, the chlorella pyrenoidosa white mutant strain is preserved in the China General Microbiological Culture Collection Center on September 11, 2024, the preservation number is CGMCC N0.46151, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. Compared with an original strain FZU65, the chlorella pyrenoidosa white mutant strain FZU65-W1 has the advantages that the growth speed of algae cells under the heterotrophic condition is higher, and meanwhile, the cells are white due to the fact that chlorophyll and carotenoid are not synthesized in the cells. The chlorella pyrenoidosa white mutant strain FZU65-W1 can be used for producing high-quality protein through heterotrophic fermentation, so that not only can a later-stage protein extraction and preparation process be simplified, but also the sensory acceptance of algae powder can be improved, and a high-quality germplasm resource is provided for industrial production and application of microalgae protein.

Provided herein are pharmaceutical compositions comprising carotenoids, including liposomes that encapsulate carotenoids including ionizable carotenoids such as trans-crocetin. The provided compositions have uses in treating diseases, disorders and conditions associated with, but not limited to, infection, endotoxemia, inflammation, sepsis, ischemia, hypoxia, shock, stroke, lung injury, wound healing, traumatic injury, reperfusion injury, cardiovascular disease, kidney disease, liver disease, inflammatory disease, metabolic disease, pulmonary disorders, blood related disorders and hyperproliferative diseases such as cancer. Methods of making, delivering, and using the pharmaceutical compositions are also provided.

The invention relates to the technical field of carotenoid cleavage dioxygenase, and discloses a carotenoid cleavage dioxygenase mutant and application thereof. In order to improve the efficiency of converting carotenoid in tobacco into aroma molecule beta-ionone, specific mutation of characteristic sites is carried out on carotenoid cleavage dioxygenase (PhCCD1) from petunia, that is, serine (S) at the 428 site of the carotenoid cleavage dioxygenase is mutated into cysteine (C) or tyrosine (Y) to obtain a mutant S428C or S428Y, and the mutant S428C or S428Y is converted into beta-ionone. The yield of the beta-ionone converted from the carotenoid is greatly improved. Experiments prove that the yields of beta-ionone of S428C and S428Y respectively reach 54.2 mg / L and 50.6 mg / L, which are respectively increased by 69.7% and 58.5% compared with those of a wild strain WT.

The present invention belongs to the technical field of microbial fermentation, and discloses a method for co-producing DHA and carotenoids by schizochytrium fermentation, wherein the method uses schizochytrium as a production strain to produce polyunsaturated fatty acids and carotenoids, adds an NADPH oxidase inhibitor to the fermentation medium, and adopts a fermentation method with staged temperature control. The present invention combines staged temperature-changing fermentation with the addition of an NADPH oxidase inhibitor during microbial fermentation, thereby achieving the co-production of DHA and carotenoids during schizochytrium fermentation, thereby obtaining microbial oils with high DHA and carotenoid content.

The utility model relates to a microwave-assisted device for preparing a carotenoid microcapsule preparation. The microwave-assisted device comprises an emulsifying tank, a microwave cover and a forming tower, the emulsifying tank comprises a feeding bin, a stirring device and a heating device; the emulsifying tank is sleeved with the microwave cover, microwave generators are further arranged on the two sides of the inner wall of the microwave cover, one side of each microwave generator is fixed to the microwave cover, and the other side of each microwave generator is fixed to the outer wall of the emulsifying tank; the microwave generating device is matched with the heating device in the emulsifying tank, so that the emulsifying efficiency of the prepared raw materials can be improved in a relatively short time. The bottom of the emulsifying tank is communicated with the forming tower through a heating pipeline, a discharge port is formed below the forming tower, and a stock bin trolley is arranged under the discharge port; the fully emulsified preparation raw materials are atomized and dried by the forming tower, and are gathered into the bin vehicle through the discharge port, so that the integration of mixing and emulsifying of the raw materials and preparation of the microcapsule preparation is realized.

The present disclosure relates to the field of microbial technology and provides a Haematococcus pluvialis KM2-1 and its applications. The present disclosure provides a Haematococcus pluvialis ( Haematococcus lacustris ) KM2-1, which was deposited at the China Center for Type Culture Collection on April 3, 2025, and its deposit number is CCTCC NO: M 2025704; the present disclosure also provides an application of Haematococcus pluvialis KM2-1 in enriching carotenoids; the present disclosure also provides an application of Haematococcus pluvialis KM2-1 in preparing products rich in carotenoids. The Haematococcus pluvialis KM2-1 provided by the present disclosure has good tolerance to light stress and can promote the accumulation of carotenoids under high-intensity light stress conditions, thereby better enriching carotenoids.

The invention relates to the technical field of gene engineering, in particular to a tomato SlHXK1 gene and application of the tomato SlHXK1 gene to improvement of weak light tolerance of tomato seedlings. The nucleotide sequence of the tomato SlHXK1 gene is as shown in SEQ ID No. 1. Research finds that the overexpression of the SlHXK1 gene can improve the activity of sucrose anabolic enzymes in tomato stems and increase distribution of dry substances to leaves, so that the content of non-structural carbohydrates in tomato leaves under weak light is increased, the content of carotenoids and soluble proteins in the leaves is increased, and the photosynthetic ability of the leaves is enhanced; therefore, the adverse effect of weak light on dry matter accumulation of tomato seedlings is reduced, the accumulation rate of dry matter of leaves after illumination recovery is promoted, and the weak light tolerance of tomato plants is improved. Therefore, the invention provides a new gene and method for improving the weak light tolerance of the tomatoes, and has important significance for guaranteeing high yield, high quality and annual supply of the tomatoes in China.

The invention discloses a method for increasing the content of carotenoid in cucumbers, a related biological material and application. The invention discloses a method for increasing the content of carotenoid in cucumbers. The content of carotenoid in cucumbers is increased by knocking out CsNCED4 genes. The CsNCED4 gene is as shown in SEQ ID No.1 (Sequence Identifier Number 1). The CsNCED4 gene is knocked out through a CRISPR / Cas9 (clustered regularly interspaced short palindromic repeats / CRISPR associated protein 9) technology, and the used target sequences are shown as SEQ ID NO.3 and SEQ ID NO.4. The recombinant expression vector comprises a complementary sequence of the target spot sequence in the method. The recombinant microorganism comprises the target sequence in the method or the recombinant expression vector. The invention provides a method for rapidly creating a cucumber material with high carotenoid content, and provides a theoretical basis and a practice mode for improving the nutritional quality of cucumbers.

The invention discloses a stress-resistant growth-promoting stable fertilizer and a preparation method thereof, and belongs to the technical field of bio-organic fertilizers. The bio-organic fertilizer is prepared by taking soybean meal leavening, pomegranate peel residues, eucommia ulmoides leaf residues, a compound microorganism bacterium solution and a microorganism activating agent as raw materials, two high-salt-tolerance growth-promoting microorganism strains are screened to form a functional microorganism, madura anactinomyces activates the enzyme production activity of salt-tolerant bacillus by secreting metabolite, and the biological organic fertilizer is prepared. And organic matters decomposed by the salt-tolerant bacillus reversely provide a growth substrate for the madura anactinomycetes to form a metabolism reciprocal relationship. The fertilizer provided by the invention can effectively regulate and stabilize soil activity and promote crop growth, can effectively improve plant stress resistance related enzyme activity, root activity and carotenoid content in leaves under salt stress, improves resistance of soybean plants to salt stress adversity conditions, and has wide development potential and application value.

The invention relates to the technical field of crop detection, in particular to a crop drought stress degree evaluation method and device and electronic equipment, and the method comprises the following steps: collecting Raman spectrum data of a crop leaf sample at a plurality of point locations; according to the Raman spectrum data of each point, obtaining a plurality of Raman spectrum peaks of the crop leaf sample on each point; obtaining a Raman spectrum characteristic peak corresponding to each drought stress marker from the plurality of Raman spectrum peaks; the drought stress marker comprises carotenoid and anthocyanin; according to the area of the Raman spectrum characteristic peak of each drought stress marker, obtaining a drought stress Raman spectrum index of the crop leaf sample at each point, and further obtaining a drought stress degree evaluation result of the crop leaf sample. Through the method, the problems of low sensitivity and poor accuracy of early drought stress identification of crops in related technologies are solved.

The invention discloses a plant leaf physicochemical parameter inversion method based on spectrum two-dimensional characterization and multi-task deep learning, and belongs to the technical field of plant physiological detection. The method comprises the following steps: constructing a hyperspectral plant leaf data set; preprocessing a hyperspectral plant leaf data set; reconstructing the preprocessed one-dimensional spectral vector into a two-dimensional image; taking the generated two-dimensional image as input, constructing a multi-task learning model, and performing training and evaluation; and applying the trained model to a test set to obtain an inversion result of the physical and chemical parameters of the leaf. The method solves the problem of parameter inversion interference caused by the same-spectrum foreign matter phenomenon, achieves the precise modeling of local fine feature extraction and global context relation, solves the inversion precision bottleneck caused by spectral response overlapping among parameters, improves the distinguishing capability of the model for easily confused parameters such as chlorophyll and carotenoid, and improves the accuracy of the model. And finally, high-precision and robust synchronous inversion of the physical and chemical parameters of the plant leaves is realized.

The invention relates to the technical field of scenic spot plant growth monitoring, and particularly discloses a scenic spot plant growth intelligent monitoring method and system, and the method comprises the steps: carrying out the multi-band image data collection of ornamental vegetation, and obtaining leaf spectrum purification data; extracting wave band response curves of anthocyanin, carotenoid and chlorophyll according to the spectral purification data to obtain pigment wave band characteristic data; performing spectrum aliasing sensitivity analysis on the pigment wave band characteristic data to obtain spectrum aliasing nonlinear characteristic data; carrying out differentiation attribution calculation on a wave band aliasing region between the healthy purple leaves and the iron-deficient yellowing to obtain aliasing difference attribution data; and finally obtaining vegetation pigment unmixing mapping data. Performing physiological status difference identification on the pigment unmixing mapping data to obtain a vegetation health monitoring result; the precision, high efficiency and foresight of scenic spot vegetation monitoring are technically guaranteed, the technical requirements of modern scenic spot vegetation management are met, and losses caused by vegetation health problems are effectively reduced.

The invention provides application of riboflavin in inhibition of postharvest yellowing of broccoli, discovers that yellowing and aging of broccoli can be effectively inhibited by soaking broccoli with a riboflavin-containing solution, and the mechanism of riboflavin in inhibition of broccoli yellowing is deeply studied. The riboflavin is found to be capable of inhibiting expression of chlorophyll degradation genes and chlorophyll aging genes in the broccoli and delaying synthesis of carotenoids by the broccoli, so that yellowing and aging of the broccoli are effectively inhibited. The broccoli treated by the method disclosed by the invention has an excellent treatment effect, and yellowing and aging of the broccoli can be obviously delayed and the shelf life can be prolonged when the broccoli is stored at normal temperature, so that the method has great significance in storage of the broccoli and is also greatly helpful for production and sales of the broccoli.

The invention discloses application of an SlBRL2 gene in improvement of tomato fruit quality, and belongs to the technical field of plant genetic engineering. According to the invention, the gene SlBRL2 related to the tomato fruit quality is found, and the CDS sequence of the gene SlBRL2 is as shown in SEQ ID NO. 1. It is found for the first time that the knockout of the SlBRL2 gene can significantly improve the tomato fruit shape index, the fruit hardness, the soluble solid content, the total sugar content and the carotenoid content, the taste and the nutritional quality of tomatoes are improved while the storage resistance and the transportation resistance of the tomatoes are improved, and a new direction is provided for tomato germplasm breeding.

The invention discloses tobacco carotenoid monooxygenase BCM for catalytic synthesis of beta-ionone and application of the tobacco carotenoid monooxygenase BCM in tobacco leaf quality improvement, and belongs to the technical field of genetic engineering. The amino acid sequences of the tobacco carotenoid monooxygenase BCM are as shown in SEQ ID NO. 1 to SEQ ID NO. 10. The carotenoid monooxygenase BCM disclosed by the invention has relatively good enzymatic activity and can be applied to catalyzing beta-carotene to generate beta-ionone, and the production capacity of an expression strain disclosed by the invention is stable and sustainable; when the recombinant engineering bacterium expressing the BCM is applied to tobacco leaf fermentation, the content of beta-ionone in tobacco leaves can be increased, the smoking quality of the tobacco leaves is improved, and the recombinant engineering bacterium shows a wide application prospect in the fields of tobacco leaf fermentation and quality improvement and is expected to promote transformation and upgrading of the tobacco industry.

The invention provides a non-destructive detection method for the content of chlorophyll and carotenoid in tobacco leaves, and belongs to the technical field of image data process.The method comprises the steps that a standardized image collection environment is constructed, and RGB images are obtained through an industrial area-array camera; performing threshold segmentation, contour extraction and rotation correction by using an HSV color space to obtain a standardized leaf image; a double-branch CNN network structure is constructed, and three attention mechanisms of channel attention, space attention and cross attention are integrated; training the network through an Adam optimizer, and evaluating the performance of the model by using a decision coefficient, a root-mean-square error and a relative analysis error; and finally, carrying out visual analysis by adopting a Grad-CAM technology, verifying the recognition effect of the model on pigment distribution, and realizing high-precision non-destructive rapid detection from image data to pigment content.

The invention provides an edible coating fresh-keeping liquid suitable for low-temperature refrigeration of harvested tropical fruits and a storage and fresh-keeping method. The low-temperature cold storage edible film coating fresh-keeping liquid is soybean oil. When bananas or mangoes are coated with the edible coating liquid for low-temperature refrigeration, the occurrence of fruits under low-temperature refrigeration can be effectively inhibited, and the sensory quality and shelf life of the fruits can be maintained. Bananas or mangoes are subjected to soybean oil coating treatment, so that the relative conductivity of fruit tissues can be effectively reduced, and the integrity of fruit cell membranes is maintained; according to the present invention, the production of H2O2, O2 <-> and MDA can be significantly reduced, the activity of POD can be inhibited, and the activity of PPO, SOD and CAT can be enhanced so as to activate the anti-oxidation defense system of fruits, reduce the oxidative damage caused by cold injury, and enhance the accumulation of osmotic regulation substances; the content of antioxidant carotenoid can be increased, so that the stress resistance of the fruits is improved, and the cold injury of the fruits in the low-temperature storage process is reduced.

The invention relates to the technical field of gene engineering, in particular to a cynanchum thesioides CtPDS gene as well as a VIGS virus vector and a silencing method of the cynanchum thesioides CtPDS gene. The invention provides phytoene dehydrogenase of cynanchum thesioides. The phytoene dehydrogenase has an amino acid sequence as shown in SEQ ID NO. 1. The invention discloses an amino acid sequence of cynanchum thesioides phytoene dehydrogenase and a sequence of a coding gene CtPDS of the cynanchum thesioides phytoene dehydrogenase, and provides a target nucleic acid molecule for silencing the CtPDS gene, a VIGS silencing system and a silencing method on the basis of the amino acid sequence of the cynanchum thesioides phytoene dehydrogenase. The leaf CtPDS gene expression level of a CtPDS gene silent plant constructed by the method provided by the invention is remarkably reduced, the silence efficiency reaches 56-65%, the chlorophyll and carotenoid contents are remarkably reduced, the leaf has a whitening phenotype, and a technical support is provided for developing functional research of cynanchum thesioides key genes.

To develop a molecular marker related to carotenoid accumulation for the Patinopecten yessoensis variety "Haidu Jinbei" and to provide a method for maintaining population quality.SOLUTION: An InDel molecular marker related to carotenoid accumulation of Patinopecten yessoensis and an application thereof are disclosed. A base sequence of the InDel molecular marker in carotenoid accumulating Patinopecten yessoensis and a base sequence of the InDel molecular marker in common Patinopecten yessoensis are disclosed. Furthermore, an upstream primer sequence and a downstream primer sequence of a primer pair for amplifying the InDel molecular marker related to carotenoid accumulation of Patinopecten yessoensis are disclosed. By detecting the InDel molecular marker, homozygous Patinopecten yessoensis individuals capable of accumulating carotenoids can be conveniently and rapidly screened in a seedling stage and from parents, and effective technical support is provided for breeding of "Haidu Jinbei" and optimization of characters.SELECTED DRAWING: Figure 2

Embodiments of the present disclosure provide molecular markers for germplasm identification and selection of carotenoid-rich bay scallops. The molecular markers include SNP1, SNP2, SNP3 and SNP4. Embodiments of the present disclosure also provide a group of primers as shown in SEQ ID NO. 5, SEQ ID NO. 6 and SEQ ID NO. 7, SEQ ID NO. 8 for the molecular markers. The disclosure provides a rapid, simple, and batch method for the identification and screening of carotenoid-rich bay scallops at a seedling stage and an adult scallop stage, which can effectively reduce the cultivation cost of carotenoid-rich scallops, improve the efficiency of farming and breeding, and has a broad market application prospect.

The invention discloses a method for regulating the body color of ornamental fish, and belongs to the technical field of aquaculture. According to the method disclosed by the invention, the aquarium fishes in the fry stage and the juvenile stage are soaked with thyroid hormone, so that the excessive thyroid hormone can obviously reduce the number of haematochrome cells and the size of pigment particles of the aquarium fishes in different stages, and reduce the content of carotenoid; when the synthesis of thyroid hormone is inhibited, gene expression of melanocytes and related synthetic pathways can be promoted, and meanwhile, the carotenoid level is increased. The inhibition effect of thyroid hormone on scatophagus argus haematochrome cell development and carotenoid accumulation is determined for the first time, the knowledge blank in the field is filled, and important guidance information is provided for body color regulation and genetic improvement of aquarium fish and other aquatic animals in the future.

The invention discloses a method for increasing the esterified carotenoid content of Chinese wolfberry. The method comprises the following steps: S1, picking Chinese wolfberry fruits; s2, on the day when the Chinese wolfberry fruits are picked, the Chinese wolfberry fruits are soaked in a melatonin aqueous solution for 15 min to 30 min, and the concentration of melatonin in the aqueous solution is 40 [mu] M to 60 [mu] M; s3, draining water after soaking, and performing breathable packaging and storage. The melatonin solution with proper concentration is adopted to soak the medlar fruits and then the medlar fruits are refrigerated and stored, the expression quantity of carotenoid esterifying enzyme genes in the medlar fruits is obviously higher, and the content of esterified carotenoids is also obviously higher, so that the loss of main bioactive components in the low-temperature storage period of the medlar fruits is avoided; the nutritional quality of the Chinese wolfberry fruits is guaranteed, the bright color of the Chinese wolfberry fruits can be maintained, and the commodity value of the Chinese wolfberry fruits is guaranteed.

The present disclosure relates to compositions and methods for producing carotenoids. The present disclosure provides genetically modified cells (e.g., microbes) that comprise a transgene encoding a ferredoxin protein, and methods of making and using said genetically modified cells. The present disclosure also provides genetically modified cells (e.g., microbes) that comprise a transgene encoding a CBP or a genetic modification capable of increasing the expression of a CBP, and methods of making and using said genetically modified cells.

The invention discloses a tobacco carotenoid dioxygenase NtCCD10 mutant as well as a genetically engineered bacterium and application thereof, and belongs to the technical field of oxidation aroma production of carrots. The thermal stability of the mutant NtCCD10-G189P-K279R disclosed by the invention is obviously improved compared with that of the NtCCD10, and the residual enzyme activity of the NtCCD10-G189P-K279R is 1.25 times that of the NtCCD10 under the condition that the mutant NtCCD10-G189P-K279R is incubated for 30 minutes at the temperature of 50 DEG C. A recombinant strain is constructed based on different carotenoid dioxygenase, and beta-carotene can be cracked to produce an aroma substance beta-ionone; the beta-carotene degradation rate of the constructed recombinant strain reaches 54.59% or above within 24 h, and the yield of beta-ionone reaches 20.721 mg / L or above; after the recombinant strain is sprayed to fermented tobacco shreds, the tobacco shred fragrance can be obviously improved, and the tobacco leaf quality is improved.

The invention relates to a feed additive for producing high-nutrition low-cholesterol eggs. The feed additive comprises natural grains, microalgae and a microalgae extract, and is prepared from the following raw materials in percentage by weight: 20-70% of corn, 5-10% of wheat bran, 5-10% of soybean meal, 5-20% of spirulina powder, 5-20% of chlorella powder and 5-20% of haematococcus pluvialis. The feed additive is added into laying fowl feed according to the proportion of 1-2wt%, the feed additive and the laying fowl feed are uniformly mixed and then fed, low-cholesterol poultry eggs rich in protein, algae source carotenoid, calcium, magnesium, zinc, iron and other nutritional ingredients can be stably obtained after the laying fowl feed is fed for 2 weeks, compared with common poultry eggs, the cholesterol content can be reduced by 26%, and the poultry eggs are fine and smooth in taste, free of fishy smell and good in taste. The nutrition and quality of poultry eggs are remarkably improved, and economic benefits are improved.