274 results about "Marker gene" patented technology

The invention belongs to the technical field of biology, and discloses a reagent combination or kit for constructing intestinal organs and application of the reagent combination or kit. According to the reagent combination or the kit, intestinal organs can be obtained from cell-derived epithelial cells obtained from a donor in a non-invasive manner, and the obtained intestinal organs can be cryopreserved and recovered and can be amplified in vitro for a long time; transcriptome characteristics are similar to those of real human small intestine tissues, and typical marker genes of various small intestine pedigree cell types are highly expressed; compared with intestinal organs obtained through induction of pluripotent stem cells, the intestinal organs have more intestinal pedigree characteristics, and the intestinal function development is more mature; after being promoted to be mature, the intestinal organ also highly expresses genes related to drug absorption and metabolism, has drug absorption capability similar to that of an immortalized intestinal cell line, but more prominently shows intestinal cell lineage characteristics, is closer to an intestinal environment in a real human body, and can be used for screening intestinal disease drugs; the intestinal barrier function is realized.

The invention provides an enhancer RNA (Ribonucleic Acid) molecule MZGAe1 and application thereof, and relates to the technical field of biology. The invention provides an enhancer RNA (Ribonucleic Acid) molecule MZGAe1. The nucleotide sequence of the enhancer RNA molecule MZGAe1 is as shown in SEQ ID NO: 1; meanwhile, the invention further provides a specific sgRNA sequence for activating the molecule in a targeted manner and a recombinant vector of the specific sgRNA sequence. Expression of endogenous MZGAe1 of cells is specifically activated by adopting a CRISPR activation technology, and conversion of mouse embryonic stem cells to bicellular-like cells can be efficiently promoted. By providing a brand-new endogenous targeted MZGAe1 accurate activation tool, the efficient transformation of wild mouse embryonic stem cells to bicellular cells can be realized only under the condition of endogenous activation of single enhancer RNA molecule MZGAe1, the proportion is at least increased by 5%, the expression of a totipotent marker gene ZSCAN4 is activated, and the expression of the totipotent marker gene ZSCAN4 is promoted. And an efficient and specific brand-new research tool and scheme are provided for researching zygote genome activation, embryonic development early events and cell reprogramming.

The present invention relates to the field of tissue-derived cell identification, in particular to a method for identifying tissue-derived cells in the body fluid based on single-cell sequencing technology. In the present invention, on the basis of high-throughput single-cell RNA sequencing data of cell samples obtained from body fluids, by means of reference component analysis (RCA), expression of known tissue-related marker genes, and a tissue-derived cell prediction model constructed based on logistic regression, the specific identification of tissue-derived cells in body fluids is achieved.

The invention provides a fluorescent protein selection marker staygold-ynaMr gene and application thereof, and belongs to the technical field of biological engineering. The method comprises the following steps: replacing codons of L-isoleucine in nucleotide sequences of a fluorescent protein gene staygold and a gene ynaM by using a rare codon ATA to respectively obtain the fluorescent protein gene staygold after codon replacement and the gene ynaMr after codon replacement, and connecting the two segments of genes after codon replacement by using a flexible protein peptide, and the fluorescent protein selection marker staygold-ynaMr gene is obtained. Experiments prove that the gene can obviously improve the screening efficiency of L-isoleucine high-yield strains.

The invention discloses a key marker gene-based intelligent cell type annotation method, which comprises the following steps of: constructing a static knowledge base by using known marker genes in a reference database, and endowing the marker genes with cell specific weights by using a TF-IDF method, so that the annotation accuracy and interpretability are improved. Meanwhile, under the condition that static matching is insufficient, the literature is understood through a large language model, mark information is extracted, dynamic completion of the knowledge base is achieved, the defect that updating of a traditional knowledge base is lagged is overcome, and good adaptability and expansibility are achieved. Besides, static and dynamic matching scores are fused in the annotation process, so that more robust cell type identification is realized, annotation requirements of multi-tissue, multi-species and novel cell states are adapted, high-precision and extensible cell type annotation can be realized in a scene with insufficient reference knowledge or a fuzzy sample, and the annotation efficiency is improved. And the method has good universality and practicability.

The invention discloses an oat KASP marker, a fingerprint spectrum and application, and relates to the technical field of molecular breeding. The KASP marker comprises 96 SNP (Single Nucleotide Polymorphism) sites, and the position information and the polymorphism of the KASP marker are shown in a table 1. The invention also provides a gene chip for analyzing the KASP marker and an oat variety characteristic fingerprint spectrum constructed by using the KASP marker, and the KASP marker, the gene chip and the oat variety characteristic fingerprint spectrum provided by the invention can be used for screening, identifying and tracing oat quality. The method can be used for identifying the authenticity of the seeds and the genetic relationship between the parent and offspring of the oat, and has important significance for protecting and improving the germplasm resources of the oat.

The invention discloses application of an overexpressed carp lpla gene in regulation and control of expression of cyprinid fish tissue-related genes, and relates to the field of gene engineering, in particular to application of the overexpressed carp lpla gene in regulation and control of cyprinid fish tissue-related genes. The overexpressed carp lpla gene is applied to regulation and control of contents of lipid indexes of total cholesterol TC and triglyceride TG of different tissues of cyprinid fishes. The invention further discloses application of the overexpressed carp lpla gene in regulation and control of fat marker genes lpla, ppar gamma, Fabp3, fast and acaca of livers and muscle tissues of cyprinid fishes. The invention further discloses application of the overexpressed carp lpla gene in regulating and controlling the relative expression quantity of carp liver cell fat metabolism related genes lpla, ppar gamma, Fabp3, fast, acaca, hsl and pnpla2. The overexpressed carp lpla gene can regulate and control lipid deposition in different tissues in zebra fish, and a research model is provided for lpla to regulate and control fat deposition and differentiation. According to the overexpressed carp lpla gene disclosed by the invention, lpla high-expression carp hepatocytes are obtained through a liposome transfection technology, so that fat key gene expression in the carp hepatocytes is changed.

The invention discloses application of a KASP molecular marker in identification or auxiliary identification of soybean hundred-grain weight character, and belongs to the technical field of soybean agronomic character molecular marker development and molecular marker auxiliary breeding. According to the application of the KASP molecular marker in identification or auxiliary identification of the soybean hundred-grain weight character, the genotype of the KASP molecular marker is G or A, and the KASP molecular marker is located at the 7703700bp position of the No.6 chromosome of a soybean genome Wm82. A2. V1. The obtained KASP marker for regulating and controlling the hundred-grain weight character gene of the soybean is suitable for rapid screening and molecular marker-assisted breeding of the relative height of the hundred-grain weight of the soybean. The molecular marker can detect genotypes of materials with different hundred-grain weights in natural populations, can also be used for molecular-assisted selective breeding of soybean materials with different hundred-grain weights, replaces large-scale phenotypic screening, reduces field workload, and greatly improves screening accuracy and efficiency.

The invention discloses a structural variation molecular marker located on a pig chromosome 7 and related to the lactation ability of a sow. The structural variation molecular marker is a DNA (deoxyribonucleic acid) fragment which is inserted between the 112024896 bp site and the 112024896 bp site on a chromosome 7 of an international pig reference genome 11.1 version, wherein the nucleotide sequence of the DNA fragment is shown as SEQ ID NO: 1; the genotype of the gene is T / T, T / INS and INS / INS. The structural variation molecular marker provided by the invention obviously influences the total weight gain character and the lactation ability character of the 7-day-old sow, and by breeding the pig with the structural variation molecular marker genotype of T / T, the lactation ability of the sow can be obviously improved, and the survival rate and the weaning weight of piglets are increased.

The invention relates to the technical field of biological detection, and discloses a reagent composition and a kit for detecting cervical cancer and precancerous lesions of cervical cancer and application of the reagent composition and the kit. The reagent composition provided by the invention is used for detecting the methylation degree of a specific marker gene, and the detection sensitivity and specificity of cervical cancer and high-grade cervical cancer precancerous lesions are at a relatively high level. When the reagent composition is used for screening cervical cancer and precancerous lesions of HPV positive people, compared with conventional cytological detection, the colposcope referral rate is greatly reduced, meanwhile, the detection times for definite diagnosis of high-grade cervical lesions or cervical cancer are reduced, and the waste of medical resources and the examination burden of patients are reduced.

The invention discloses application of a compound with a benzo-aza structure in preparation of a medicine for inhibiting osteoclast formation, the structure of the compound is shown as a formula (1), and R substituent is one of H, OCH3, Br, F and i-Pr. Cell experiments prove that the compound with the benzo-aza structure has a remarkable inhibiting effect on RANKL-induced osteoclast differentiation and marker gene expression, can be used as a candidate medicine which is novel in structure and is used for inhibiting osteoclast formation, and is used for targeting osteoclast to treat osteoporosis related diseases.

The invention discloses a primer composition and a kit for identifying testudinate species and application of the primer composition and the kit. The primer composition comprises primers (SEQ ID NO.1-6) for targeted amplification of specific marker genes 16S, COI and Cytb of testudinate mitochondrial species and an enrichment universal primer (SEQ ID NO.7). According to the invention, full lengths of three mitochondrial genes 16S, COI and Cytb are taken as targets, a specific primer group added with an enrichment tag and an enrichment primer are designed, and a targeted nanopore sequencing method capable of realizing testudinate species identification is established and optimized, and the method can simultaneously capture testudinate mitochondrial species specific marker gene information of a family species level, and can be used for identifying testudinate species. The problem that the existing DNA bar code is difficult to accurately identify the testudinate species due to the lack of database information is effectively solved; and each marker gene can obtain a complete coding sequence, so that genetic information is richer, and the method can be used for geographical traceability analysis of testudinate species.

The present application relates to the field of agricultural biotechnology, and specifically discloses a kind of corn ear roughness related molecular marker and its application.The present application provides the application of corn ear roughness molecular marker in ear roughness corn molecular marker assisted breeding, the molecular marker is Zm00001d020000 gene, the nucleotide sequence of the Zm00001d020000 gene is as shown in SEQ ID NO:1, the expression amount of the gene is positively correlated with the corn ear roughness trait, multiple SNP sites related to ear roughness are screened out by GWAS analysis, and simultaneously, QTL point analysis is used to be commonly positioned to SNP_84054216 located on the 7th chromosome, and the marker gene Zm00001d020000 for regulating ear roughness is excavated from the site, and the results of the present application provide technical support for molecular marker breeding of rough ear corn varieties.

The present application relates to the field of genetic engineering, and in particular to a vector for analyzing plant promoter expression specificity, a preparation method and application thereof. The T-DNA region of the vector comprises, from 5' end to 3' end, a callus-specific promoter-driven selection marker gene expression cassette and a reporter gene expression cassette with a multiple cloning site; the callus-specific promoter-driven selection marker gene expression cassette comprises a callus-specific promoter, a selection marker gene coding sequence and a terminator. The vector of the present application utilizes a rice callus-specific promoter to drive the expression of a selection marker gene, can greatly reduce the non-specific interaction with the promoter of a target gene, improve the specificity of the expression of the target gene, and effectively reduce the biological safety risk caused by the selection marker gene in a transgenic plant, and has important application value in basic theoretical research and molecular breeding.

The invention belongs to the technical field of biomolecular markers, and particularly relates to a cross-animal general skeleton-derived hematopoietic stem cell marker gene set and a screening method thereof. According to the invention, a bone-derived hematopoietic stem cell marker gene set universal across animal categories is constructed for the first time, and the bone-derived hematopoietic stem cell marker gene set comprises at least five of Cdc42, Cbx, Tfam, Denr, Mcts1, Ak2, Ruvbl, Ahcy, Nna and Vdac; the hematopoietic stem cell marker gene set is obtained through cross-species homologous gene screening, the defect that a traditional vertebrate marker has no orthohomology in invertebrates is overcome, accurate recognition of HSC in shells is achieved, the hematopoietic stem cell marker gene set has species universality and cell specificity, the immune state of aquatic animals can be evaluated, disease-resistant breeding can be guided, and the application prospect is wide. And molecular evidence is provided for analyzing an evolution path of a hematopoietic system from invertebrates to vertebrates.

The invention discloses application of a PRTFDC1 gene in promoting porcine skeletal muscle satellite cell differentiation, myotube formation and skeletal muscle development. According to the invention, overexpression and knock-down experiments of the PRTFDC1 are respectively carried out in pig MuSCs, and immunofluorescence, qRT-PCR and Western blot analysis are carried out on cells subjected to induced differentiation, so that the PRTFDC1 is effectively shown to play a positive regulation role in a pig MuSCs differentiation process, and a new marker gene which can be applied to pig molecular breeding and growth performance evaluation is provided; and a novel molecular target and a novel detection tool are provided for muscle development regulation and biological breeding.

The invention relates to the technical field of biology, in particular to a gene co-expression network-based sepsis marker screening method and application. The screening method comprises the following steps: screening differential expression genes; performing hierarchical clustering analysis on each differential expression gene to combine each differential expression gene into a plurality of target gene modules; and screening out hub genes from each target gene module. According to the method, gene expression data and clinical feature data are combined, the accuracy of screening sepsis marker genes is improved, possible abnormal genes irrelevant to the pathophysiological process of sepsis are avoided, gene modules are divided more finely through primary clustering analysis and secondary clustering analysis, GO function analysis is combined, and the accuracy of screening the sepsis marker genes is improved. According to an analysis result, selection of a GO term subset is optimized, and a step length set by primary clustering is corrected, so that the fine granularity and accuracy of analysis are improved, division of gene modules is optimized, and more refined treatment and screening of markers of sepsis are realized.

To provide a method for evaluating the quality of neuroretina for transplantation and neuroretina sheets for transplantation selected by said method. [Solution] The present invention provides a method for evaluating the quality of neuroretina for transplantation, comprising: extracting a part or all of a cell aggregate containing a neuroretina having an epithelial structure derived from pluripotent stem cells as a quality evaluation sample; detecting the expression of neuroretinal cell-related genes and non-neuroretinal cell-related genes in the quality evaluation sample; and determining, if the expression of neuroretinal cell-related genes is observed and the expression of non-neuroretinal cell-related genes is not observed, that (1) a neuroretina (neuroretina for transplantation) from the same cell aggregate as the cell aggregate containing a part of the quality evaluation sample, (2) a neuroretina (neuroretina for transplantation) from the same lot as the cell aggregate containing a part of the quality evaluation sample, or (3) a neuroretina (neuroretina for transplantation) from the same lot as the cell aggregate of the entire quality evaluation sample, wherein the non-neuroretinal cell-related genes include one or more genes selected from the group consisting of brain and spinal cord tissue marker genes and eyeball-related tissue marker genes.

This invention provides a method for creating a transgenic papaya strain highly resistant to papaya ringspot virus and papaya mosaic virus. The method involves using PRSV and PLDMV of papaya... CP The fusion gene fragment expression cassette and the selection marker gene expression cassette were placed in two separate T-DNA domains and inserted into a plant expression vector. This vector was then introduced into papaya to obtain a papaya line without the selection marker; alternatively, PRSV and PLDMV were used. CP A fusion gene expression cassette and its reverse sequence were tandemly inserted into a plant expression vector, which was then introduced into papaya to obtain... CP A papaya strain with inversely linked gene expression cassettes. The marker-free papaya of this invention does not contain selection markers, effectively improving safety; CP Papaya with inversely linked gene expression cassettes does not require further homozygosity to enhance disease resistance.

The invention discloses application of a wheat disease-resistant factor TatrxH4 in prevention and treatment of gibberellic disease, and belongs to the technical field of genetic engineering, and a coding gene CDS sequence of the wheat disease-resistant factor TatrxH4 is shown as SEQ ID NO: 1; the amino acid sequence of the wheat disease-resistant factor TatrxH4 is as shown in SEQ ID NO: 2; the wheat disease-resistant factor TatrxH4 plays a positive regulation role in immune response of wheat to gibberellic disease, and over-expression of the wheat disease-resistant factor TatrxH4 improves the resistance of wheat to gibberellic disease. According to the invention, an agrobacterium tumefaciens-mediated method and an RNA interference technology are adopted to obtain a wheat strain of an overexpressed disease-resistant factor TatrxH4, and the disease-resistant factor TatrxH4 is identified according to the morbidity condition, disease index and expression condition of disease-resistant related marker genes of an overexpressed plant and common Fielder wheat infected with fusarium graminearum PH-1, so that the disease-resistant factor TatrxH4 is identified. The wheat disease-resistant factor TatrxH4 is known to play a positive regulation role in the immune response of wheat scab resistance, so that the wheat disease-resistant factor TatrxH4 can be used for creating a scab-resistant wheat germplasm material and plays a practical role in prevention and treatment of the wheat scab on the basis of the positive regulation role of the wheat disease-resistant factor TatrxH4.

The invention relates to a marker gene screening method and device, computer equipment, a storage medium and a computer program product. The method comprises the following steps: counting the positive rate of each candidate gene in each cell type according to a to-be-analyzed single cell gene expression matrix and cell classification information; performing data conversion on the gene expression quantity in the single cell gene expression matrix and the positive rate of each candidate gene in each cell type to obtain a feature vector of each candidate gene in each cell type; calculating a gene score value of each candidate gene in each cell type on the basis of the feature vectors; and screening each candidate gene according to the gene score value to obtain the high-specificity marker gene of each cell type. By adopting the method, the marker gene with high specific expression can be screened out, and the accuracy of marker gene screening is improved.

The invention discloses an application of an SNP (Single Nucleotide Polymorphism) molecular marker related to porcine scrotal hernia on a porcine chromosome 18, and the locus of the SNP molecular marker is rs332175156 and corresponds to Cgt at the 15224983 bp position on the chromosome 18 of an international porcine reference genome version 11.1; t mutation; the genotype of the gene is CC, CT or TT. The SNP molecular marker provided by the invention is remarkably related to the scrotal hernia morbidity of pigs, and the scrotal hernia morbidity of pigs with the genotype of TT of the SNP molecular marker is lower than that of pigs with the genotype of CT or CC. By breeding the pig with the SNP molecular marker genotype being TT, the improvement progress of the genetic defect of the pig scrotal hernia can be accelerated, and the morbidity of the scrotal hernia is reduced.

The invention relates to the technical field of cosmetics, in particular to a compound active peptide with functions of resisting aging and whitening skin and application of the compound active peptide. Comprising acetyl hexapeptide-1, nonapeptide-1, acetyl tetrapeptide-11, hexapeptide-9 and palmitoyl hexapeptide-12. The compound peptide disclosed by the invention can play a synergistic anti-aging and whitening role on an epidermal layer and a corium layer, and has multiple advantages; the compound peptide acts on epidermal layer keratinocytes, dermal layer fibroblasts and B16F10 cells, and can be used for detecting the senescence of the cells by influencing the expression quantity of cell senescence marker genes, influencing the cell cycle, influencing the expression quantity of extracellular matrix related proteins, influencing melanin synthesis and influencing the secretion quantity of acetylcholine in the cells. Multiple skin anti-aging and whitening effects synergistically exerted on the epidermal layer and the corium layer are achieved.

The invention belongs to the technical field of biological medicines, and particularly relates to application of miR-206-3p in regulation and control of skeletal muscle atrophy, and the change condition of the miR-206-3p level is measured by constructing an in-vitro cancer cachexia myotubular atrophy model; it is verified that in-vitro addition of miR-206-3p mimics relieves in-vitro myotube atrophy induced by a cancer conditioned culture medium, inhibits expression of a myotube marker gene and inhibits proteolysis and metabolism pathways and cell apoptosis of myotubes, so that myotube atrophy induced by cancer cachexia is inhibited. The invention opens up a new thought for the research of cachexia pathogenesis, and also provides a potential new target for the research and development of cachexia treatment and / or cachexia prevention drugs.

The invention belongs to the technical field of biological medicine, and particularly discloses a small molecule compound SR4370 for promoting hair follicle growth and regeneration and application thereof. High-throughput screening finds that SR4370 can induce dermal fibroblasts to be reprogrammed into DP-like cells with characteristics of hair papilla cells and up-regulate ALP, WNT5A and other marker genes, and in-vivo and in-vitro experiments prove that SR4370 can activate resting hair follicles, promote in-vitro hair follicle growth and enhance the regeneration capacity of dermal stem cells. The invention provides a safe and efficient hair follicle regeneration strategy, and provides a new tool for alopecia treatment and tissue engineering.

The invention belongs to the technical field of agricultural bioengineering, and relates to application of a wheat disease-resistant factor TaPKS26 in prevention and treatment of leaf rust. The CDS sequence of the encoding gene of the wheat disease-resistant factor TaPKS26 is shown as SEQ ID NO: 1, a transgenic wheat strain of the overexpressed gene TaPKS26 is obtained by adopting an agrobacterium-mediated method, and the transgenic wheat strain of the overexpressed gene TaPKS26 is proved to have enhanced resistance to wheat leaf rust compared with Fielder wheat after being infected with physiological races THT and PHT, and the wheat leaf rust resistance is improved. The expression quantity of the disease-resistant related marker gene is up-regulated, which indicates that the wheat disease-resistant factor TaPKS26 plays a positive regulation role in wheat leaf rust-resistant defensive reaction. The invention provides new gene resources and technical thoughts for the cultivation of leaf rust resistant varieties.

The invention discloses a filamentous fungus genetic transformation system taking an endogenous KatG1 gene as a selection marker, a method and application. The method comprises the following steps: carrying out linear DNA fragment construction on an endogenous Pgpd promoter, an endogenous KatG1 gene, a target promoter and a target gene of filamentous fungi, then transforming the constructed linear DNA fragment into filamentous fungi cells, and finally screening through a menadione-containing culture medium to obtain the directionally improved filamentous fungi strain containing the target gene. According to the invention, the endogenous KatG1 gene of the fungus is used as a selection marker gene and menadione is used as screening resistance to construct a novel selection marker based on an endogenous gene and a genetic transformation system without exogenous DNA (Deoxyribose Nucleic Acid) such as a carrier and the like, so that the method has the advantages of universality, high efficiency and safety; an environment-friendly solution is provided for directional genetic improvement of filamentous fungi, and the method has important commercial application potential in directional breeding of fungus strains in the fields of agriculture, industry and the like.

The invention discloses application of an SNP molecular marker located on a pig chromosome 9 and related to scrotal hernia. The locus of the SNP molecular marker is rs318420719 and corresponds to Ggt at the 129354398 bp position on a chromosome 9 of an international pig reference genome version 11.1; a mutation; the genotype of the gene is GG, GA or AA. The SNP molecular marker provided by the invention is remarkably related to the scrotal hernia morbidity of pigs, the scrotal hernia morbidity of the pigs with the genotype of GA of the SNP molecular marker is lower than that of the pigs with the genotype of GG or AA, and by breeding the pigs with the genotype of GA of the SNP molecular marker, the scrotal hernia morbidity can be reduced, and the improvement progress of pig genetic defects can be accelerated.