171 results about "Marker gene" patented technology

The invention discloses a key marker gene-based intelligent cell type annotation method, which comprises the following steps of: constructing a static knowledge base by using known marker genes in a reference database, and endowing the marker genes with cell specific weights by using a TF-IDF method, so that the annotation accuracy and interpretability are improved. Meanwhile, under the condition that static matching is insufficient, the literature is understood through a large language model, mark information is extracted, dynamic completion of the knowledge base is achieved, the defect that updating of a traditional knowledge base is lagged is overcome, and good adaptability and expansibility are achieved. Besides, static and dynamic matching scores are fused in the annotation process, so that more robust cell type identification is realized, annotation requirements of multi-tissue, multi-species and novel cell states are adapted, high-precision and extensible cell type annotation can be realized in a scene with insufficient reference knowledge or a fuzzy sample, and the annotation efficiency is improved. And the method has good universality and practicability.

The invention discloses an oat KASP marker, a fingerprint spectrum and application, and relates to the technical field of molecular breeding. The KASP marker comprises 96 SNP (Single Nucleotide Polymorphism) sites, and the position information and the polymorphism of the KASP marker are shown in a table 1. The invention also provides a gene chip for analyzing the KASP marker and an oat variety characteristic fingerprint spectrum constructed by using the KASP marker, and the KASP marker, the gene chip and the oat variety characteristic fingerprint spectrum provided by the invention can be used for screening, identifying and tracing oat quality. The method can be used for identifying the authenticity of the seeds and the genetic relationship between the parent and offspring of the oat, and has important significance for protecting and improving the germplasm resources of the oat.

The invention discloses a structural variation molecular marker located on a pig chromosome 7 and related to the lactation ability of a sow. The structural variation molecular marker is a DNA (deoxyribonucleic acid) fragment which is inserted between the 112024896 bp site and the 112024896 bp site on a chromosome 7 of an international pig reference genome 11.1 version, wherein the nucleotide sequence of the DNA fragment is shown as SEQ ID NO: 1; the genotype of the gene is T / T, T / INS and INS / INS. The structural variation molecular marker provided by the invention obviously influences the total weight gain character and the lactation ability character of the 7-day-old sow, and by breeding the pig with the structural variation molecular marker genotype of T / T, the lactation ability of the sow can be obviously improved, and the survival rate and the weaning weight of piglets are increased.

The invention relates to the technical field of biological detection, and discloses a reagent composition and a kit for detecting cervical cancer and precancerous lesions of cervical cancer and application of the reagent composition and the kit. The reagent composition provided by the invention is used for detecting the methylation degree of a specific marker gene, and the detection sensitivity and specificity of cervical cancer and high-grade cervical cancer precancerous lesions are at a relatively high level. When the reagent composition is used for screening cervical cancer and precancerous lesions of HPV positive people, compared with conventional cytological detection, the colposcope referral rate is greatly reduced, meanwhile, the detection times for definite diagnosis of high-grade cervical lesions or cervical cancer are reduced, and the waste of medical resources and the examination burden of patients are reduced.

The invention discloses a primer composition and a kit for identifying testudinate species and application of the primer composition and the kit. The primer composition comprises primers (SEQ ID NO.1-6) for targeted amplification of specific marker genes 16S, COI and Cytb of testudinate mitochondrial species and an enrichment universal primer (SEQ ID NO.7). According to the invention, full lengths of three mitochondrial genes 16S, COI and Cytb are taken as targets, a specific primer group added with an enrichment tag and an enrichment primer are designed, and a targeted nanopore sequencing method capable of realizing testudinate species identification is established and optimized, and the method can simultaneously capture testudinate mitochondrial species specific marker gene information of a family species level, and can be used for identifying testudinate species. The problem that the existing DNA bar code is difficult to accurately identify the testudinate species due to the lack of database information is effectively solved; and each marker gene can obtain a complete coding sequence, so that genetic information is richer, and the method can be used for geographical traceability analysis of testudinate species.

The present application relates to the field of agricultural biotechnology, and specifically discloses a kind of corn ear roughness related molecular marker and its application.The present application provides the application of corn ear roughness molecular marker in ear roughness corn molecular marker assisted breeding, the molecular marker is Zm00001d020000 gene, the nucleotide sequence of the Zm00001d020000 gene is as shown in SEQ ID NO:1, the expression amount of the gene is positively correlated with the corn ear roughness trait, multiple SNP sites related to ear roughness are screened out by GWAS analysis, and simultaneously, QTL point analysis is used to be commonly positioned to SNP_84054216 located on the 7th chromosome, and the marker gene Zm00001d020000 for regulating ear roughness is excavated from the site, and the results of the present application provide technical support for molecular marker breeding of rough ear corn varieties.

The present application relates to the field of genetic engineering, and in particular to a vector for analyzing plant promoter expression specificity, a preparation method and application thereof. The T-DNA region of the vector comprises, from 5' end to 3' end, a callus-specific promoter-driven selection marker gene expression cassette and a reporter gene expression cassette with a multiple cloning site; the callus-specific promoter-driven selection marker gene expression cassette comprises a callus-specific promoter, a selection marker gene coding sequence and a terminator. The vector of the present application utilizes a rice callus-specific promoter to drive the expression of a selection marker gene, can greatly reduce the non-specific interaction with the promoter of a target gene, improve the specificity of the expression of the target gene, and effectively reduce the biological safety risk caused by the selection marker gene in a transgenic plant, and has important application value in basic theoretical research and molecular breeding.

The invention discloses application of a PRTFDC1 gene in promoting porcine skeletal muscle satellite cell differentiation, myotube formation and skeletal muscle development. According to the invention, overexpression and knock-down experiments of the PRTFDC1 are respectively carried out in pig MuSCs, and immunofluorescence, qRT-PCR and Western blot analysis are carried out on cells subjected to induced differentiation, so that the PRTFDC1 is effectively shown to play a positive regulation role in a pig MuSCs differentiation process, and a new marker gene which can be applied to pig molecular breeding and growth performance evaluation is provided; and a novel molecular target and a novel detection tool are provided for muscle development regulation and biological breeding.

To provide a method for evaluating the quality of neuroretina for transplantation and neuroretina sheets for transplantation selected by said method. [Solution] The present invention provides a method for evaluating the quality of neuroretina for transplantation, comprising: extracting a part or all of a cell aggregate containing a neuroretina having an epithelial structure derived from pluripotent stem cells as a quality evaluation sample; detecting the expression of neuroretinal cell-related genes and non-neuroretinal cell-related genes in the quality evaluation sample; and determining, if the expression of neuroretinal cell-related genes is observed and the expression of non-neuroretinal cell-related genes is not observed, that (1) a neuroretina (neuroretina for transplantation) from the same cell aggregate as the cell aggregate containing a part of the quality evaluation sample, (2) a neuroretina (neuroretina for transplantation) from the same lot as the cell aggregate containing a part of the quality evaluation sample, or (3) a neuroretina (neuroretina for transplantation) from the same lot as the cell aggregate of the entire quality evaluation sample, wherein the non-neuroretinal cell-related genes include one or more genes selected from the group consisting of brain and spinal cord tissue marker genes and eyeball-related tissue marker genes.

This invention provides a method for creating a transgenic papaya strain highly resistant to papaya ringspot virus and papaya mosaic virus. The method involves using PRSV and PLDMV of papaya... CP The fusion gene fragment expression cassette and the selection marker gene expression cassette were placed in two separate T-DNA domains and inserted into a plant expression vector. This vector was then introduced into papaya to obtain a papaya line without the selection marker; alternatively, PRSV and PLDMV were used. CP A fusion gene expression cassette and its reverse sequence were tandemly inserted into a plant expression vector, which was then introduced into papaya to obtain... CP A papaya strain with inversely linked gene expression cassettes. The marker-free papaya of this invention does not contain selection markers, effectively improving safety; CP Papaya with inversely linked gene expression cassettes does not require further homozygosity to enhance disease resistance.

The invention discloses application of a wheat disease-resistant factor TatrxH4 in prevention and treatment of gibberellic disease, and belongs to the technical field of genetic engineering, and a coding gene CDS sequence of the wheat disease-resistant factor TatrxH4 is shown as SEQ ID NO: 1; the amino acid sequence of the wheat disease-resistant factor TatrxH4 is as shown in SEQ ID NO: 2; the wheat disease-resistant factor TatrxH4 plays a positive regulation role in immune response of wheat to gibberellic disease, and over-expression of the wheat disease-resistant factor TatrxH4 improves the resistance of wheat to gibberellic disease. According to the invention, an agrobacterium tumefaciens-mediated method and an RNA interference technology are adopted to obtain a wheat strain of an overexpressed disease-resistant factor TatrxH4, and the disease-resistant factor TatrxH4 is identified according to the morbidity condition, disease index and expression condition of disease-resistant related marker genes of an overexpressed plant and common Fielder wheat infected with fusarium graminearum PH-1, so that the disease-resistant factor TatrxH4 is identified. The wheat disease-resistant factor TatrxH4 is known to play a positive regulation role in the immune response of wheat scab resistance, so that the wheat disease-resistant factor TatrxH4 can be used for creating a scab-resistant wheat germplasm material and plays a practical role in prevention and treatment of the wheat scab on the basis of the positive regulation role of the wheat disease-resistant factor TatrxH4.

The invention relates to a marker gene screening method and device, computer equipment, a storage medium and a computer program product. The method comprises the following steps: counting the positive rate of each candidate gene in each cell type according to a to-be-analyzed single cell gene expression matrix and cell classification information; performing data conversion on the gene expression quantity in the single cell gene expression matrix and the positive rate of each candidate gene in each cell type to obtain a feature vector of each candidate gene in each cell type; calculating a gene score value of each candidate gene in each cell type on the basis of the feature vectors; and screening each candidate gene according to the gene score value to obtain the high-specificity marker gene of each cell type. By adopting the method, the marker gene with high specific expression can be screened out, and the accuracy of marker gene screening is improved.

The invention discloses an application of an SNP (Single Nucleotide Polymorphism) molecular marker related to porcine scrotal hernia on a porcine chromosome 18, and the locus of the SNP molecular marker is rs332175156 and corresponds to Cgt at the 15224983 bp position on the chromosome 18 of an international porcine reference genome version 11.1; t mutation; the genotype of the gene is CC, CT or TT. The SNP molecular marker provided by the invention is remarkably related to the scrotal hernia morbidity of pigs, and the scrotal hernia morbidity of pigs with the genotype of TT of the SNP molecular marker is lower than that of pigs with the genotype of CT or CC. By breeding the pig with the SNP molecular marker genotype being TT, the improvement progress of the genetic defect of the pig scrotal hernia can be accelerated, and the morbidity of the scrotal hernia is reduced.

The invention relates to the technical field of cosmetics, in particular to a compound active peptide with functions of resisting aging and whitening skin and application of the compound active peptide. Comprising acetyl hexapeptide-1, nonapeptide-1, acetyl tetrapeptide-11, hexapeptide-9 and palmitoyl hexapeptide-12. The compound peptide disclosed by the invention can play a synergistic anti-aging and whitening role on an epidermal layer and a corium layer, and has multiple advantages; the compound peptide acts on epidermal layer keratinocytes, dermal layer fibroblasts and B16F10 cells, and can be used for detecting the senescence of the cells by influencing the expression quantity of cell senescence marker genes, influencing the cell cycle, influencing the expression quantity of extracellular matrix related proteins, influencing melanin synthesis and influencing the secretion quantity of acetylcholine in the cells. Multiple skin anti-aging and whitening effects synergistically exerted on the epidermal layer and the corium layer are achieved.

The invention belongs to the technical field of biological medicines, and particularly relates to application of miR-206-3p in regulation and control of skeletal muscle atrophy, and the change condition of the miR-206-3p level is measured by constructing an in-vitro cancer cachexia myotubular atrophy model; it is verified that in-vitro addition of miR-206-3p mimics relieves in-vitro myotube atrophy induced by a cancer conditioned culture medium, inhibits expression of a myotube marker gene and inhibits proteolysis and metabolism pathways and cell apoptosis of myotubes, so that myotube atrophy induced by cancer cachexia is inhibited. The invention opens up a new thought for the research of cachexia pathogenesis, and also provides a potential new target for the research and development of cachexia treatment and / or cachexia prevention drugs.

The invention discloses application of an SNP molecular marker located on a pig chromosome 9 and related to scrotal hernia. The locus of the SNP molecular marker is rs318420719 and corresponds to Ggt at the 129354398 bp position on a chromosome 9 of an international pig reference genome version 11.1; a mutation; the genotype of the gene is GG, GA or AA. The SNP molecular marker provided by the invention is remarkably related to the scrotal hernia morbidity of pigs, the scrotal hernia morbidity of the pigs with the genotype of GA of the SNP molecular marker is lower than that of the pigs with the genotype of GG or AA, and by breeding the pigs with the genotype of GA of the SNP molecular marker, the scrotal hernia morbidity can be reduced, and the improvement progress of pig genetic defects can be accelerated.

The invention discloses application of isobutyrate in construction of a heart failure cell model, and relates to the technical field of biology. By innovatively applying isobutyric acid or isobutyrate, a new heart failure cell model is constructed. The model treats myocardial cells by means of isobutyrate with the concentration of 0.5-5 mM, the heart failure phenotype can be efficiently induced, the effect of the concentration of 5 mM is optimal, expression of core heart failure marker genes such as Nppa, Nppb and Myh7 can be stably up-regulated, meanwhile, the area of the myocardial cells is remarkably increased, and the heart failure pathological characteristics are met. Compared with a traditional angiotensin II and other neurohormone induction model, the invention provides a non-neurohormone-dependent brand-new heart failure cell model construction path, focuses on metabolic disorder related heart failure inducements, makes up for the defect of single mechanism of the existing model, and provides a new heart failure cell model construction path. A unique tool is provided for researching myocardial damage caused by abnormal metabolism or intestinal flora metabolites.

This invention relates to the field of plant breeding. Particularly, the present invention relates to a diploid dicotyledonous crop plant comprising in its genome a variant of the DMP gene capable of inducing maternal haploid embryo formation and an endogenous dominant marker gene. The present invention further relates to a method for identifying and / or selecting maternal haploid dicotyledonous crop plants from a plurality of dicotyledonous crop plants comprising determining the absence of the phenotype associated with a dominant marker gene, a method for producing a maternal haploid plant comprising the step of pollinating a plant with the pollen of a diploid dicotyledonous crop plant according to the present invention, a method for producing a plant from the maternal haploid plant provided by the present invention and the use of the diploid dicotyledonous crop plant according to the present invention as a pollinator plant.

The invention relates to methods for predicting an outcome for a prostate cancer patient. In particular the method describes a model based on the expression levels of marker genes which can be used to predict the effectiveness of androgen deprivation therapy (ADT). The method allows for treatment suggestions, particularly for those patients where ADT is predicted not to be effective, and alternative treatment may be desirable. The invention further describes kits useful for use in the described methods, as well as composition for use in treatment of prostate cancer based on a treatment outcome prediction described herein.

The invention discloses a rumen fibroblast line as well as a construction method, a culture method and application thereof, the rumen fibroblast line G8 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: C2025332, the rumen fibroblast line G8 has a typical fibroblast-like form and can be stably passaged, and the rumen fibroblast line G8 can be used as a rumen fibroblast cell line. The cell culture medium also has the characteristic of highly expressing fibroblast marker genes and the function of tolerating and utilizing rumen core metabolite butyric acid, and the proliferation rate and metabolic activity of the cell culture medium under the condition that 15mmol / L butyric acid is used as a main energy source are obviously superior to those of glucose culture; according to the construction method of the rumen fibroblast line, the steps of function screening and molecular identification are introduced, so that the identity authenticity of the constructed cell line can be ensured; the culture method of the rumen fibroblast line is simple and effective, culture medium materials are easy to obtain, and culture conditions are easy to realize; the rumen fibroblast line can be used for rumen in-vitro research, and is beneficial to clarification of a mechanism for regulating rumen interstitial metabolism and tissue repair by butyric acid.

The invention discloses a luminescent cell for rapidly detecting drug allergy and / or anaphylactoid reaction as well as a construction method and application of the luminescent cell, and relates to the technical field of biology. The construction method comprises the following steps: transforming myeloid related cells by using a recombinant vector containing a marker gene and an HHEXA gene promoter to obtain recombinant cells; the recombinant cell is the luminescent cell. According to the invention, the HHEXA gene promoter and the marker gene are recombined and then transferred into myeloid related cells, and the occurrence of the allergy or anaphylactoid reaction can be intuitively reflected through a marker signal by utilizing the specific activation characteristic of the HHEXA gene in the allergy / anaphylactoid reaction. The luminescent cell has good stability, is suitable for multiple scenes such as drug allergy, anaphylactoid risk assessment, environmental sample analysis and the like, provides an efficient, sensitive and convenient technical tool for clinical rapid diagnosis, new drug research and development and scientific research and exploration, and can significantly reduce the detection cost and the operation threshold.

The present invention relates to a method for analysing a spermatozoon, comprising a step of extracting the nucleic acids from the spermatozoa contained in a first sample of spermatozoa previously obtained from a human subject; a step of measuring the level of expression of at least one marker gene chosen from the group consisting of AURKA, CFAP46, CCDC60, CCDC88B, HDAC4, CACNA1C, CACNA1H, CARHSP1, DNAH2 and SPATA18 from the extracted nucleic acids; and a step of determining the existence of an expression differential of the at least one marker gene in relation to a control. It also relates to a method for selecting spermatozoa and a method for evaluating the quality of a sperm.

The application discloses a food-like lactobacillus double-plasmid multi-gene editing system based on CRISPR / Cas9 technology and application thereof. The food-like lactobacillus double-plasmid multi-gene editing system comprises (1) a plasmid pCRI01 comprising the following elements: a Cas9 protein coding gene; a temperature-sensitive replicon pSC101; an sgRNA expression module targeting the replicon of the plasmid pCRI02, which is controlled by an inducible promoter Pnis; an ampicillin resistance; and (2) a plasmid pCRI02 comprising the following elements: at least one sgRNA expression module driven by a P23 promoter; a lambda-Red recombinase system comprising Exo, Beta and Gam genes; an erythromycin resistance marker gene; and a kanamycin resistance gene. The multi-gene editing system provided by the application is a small-size pCRI01 and pCRI02 double-plasmid system, and the number of transformed cells after resistance screening is significantly improved; and the gene editing efficiency mediated by the pCRI plasmid is greatly improved with the assistance of the lambda-Red recombinase system.

This application discloses a method, system, device, and medium for cell type detection. The method acquires whole-genome expression data, marker gene information, and images of a batch of cells to be detected; based on the whole-genome expression data and marker gene information, it determines the first expression level of the marker gene corresponding to each cell to be detected; based on the first expression level, it filters out a set of cells with significant expression, which includes several first cells; based on the whole-genome expression data and marker gene information of the first cells, it determines the cell type of the first cells; based on the cell types of each first cell and the image, it determines the cell type of a second cell using a K-nearest neighbor algorithm; the second cell is any other cell to be detected besides the first cells. This method can quickly and accurately determine the type information of a large number of cells and can be used in single-cell type labeling. This application can be widely applied in the field of bioinformatics.

This invention relates to the fields of synthetic biology and metabolic engineering, specifically to a Streptomyces promoter selection marker and its application. The selection marker gene is *Streptomyces lilacinus*. Streptomyces lavendulae Glutamine indigo synthase gene derived from CGMCC 4.1386 idgS This invention screens endogenous promoters of *Streptomyces freundii* using heterologously expressed idgS genes; and constructs a detection system for endogenous promoters of *Streptomyces freundii* using this scheme, which can rapidly detect endogenous promoters of *Streptomyces freundii* and the strength of each promoter.

The invention relates to the technical field of molecular biomedicine, in particular to a method for identifying enteropathogenic Escherichia coli, which comprises the following steps: screening virulence genes, determining a Cutom Markers gene, detecting mNGS of a clinical specimen, comparing off-machine data and identifying the enteropathogenic Escherichia coli. The specificity of the identification method reaches 99.9%, and the sensitivity reaches 96.8% when the detection sequence of escherichia coli is greater than or equal to 10k reads. By utilizing the method, the accuracy and applicability of enteropathogenic escherichia coli identification can be remarkably improved, and the method can be widely applied to the field of clinical microbiological detection.

The application discloses a rice OsNAR1 application of the gene or the coded protein in regulating rice resistance to Magnaporthe oryzae, and relates to the technical field of rice Magnaporthe oryzae prevention and treatment. OsNAR1 The CDS nucleotide sequence of the gene is shown as SEQ ID No. 2. OsNAR1 The application finds that the rice gene OsNAR1 plays an important role in the process of rice resistance to Magnaporthe oryzae, the number of leaf spots on the gene deletion mutant is less, the gene can be used for screening rice strains resistant to Magnaporthe oryzae, the expression amount of a basic resistance marker gene in the gene deletion mutant is significantly up-regulated, and it is indicated that OsNAR1 negatively regulates the expression of the basic resistance gene and can be used for preventing and treating Magnaporthe oryzae.

The invention belongs to the technical field of cell engineering and genetic engineering, and particularly relates to application of an FBXO32 gene in regulation and control of skeletal muscle satellite cell proliferation and feed utilization rate. According to overexpression of the FBXO32 gene in skeletal muscle satellite cells, it is found that the mRNA expression quantity of skeletal muscle satellite cell proliferation marker genes KI67 and CCND1 is remarkably increased, and the protein expression level is also remarkably improved. CCK-8 and EdU detection results also show that the FBXO32 gene or the encoded protein thereof also has a promotion effect on skeletal muscle satellite cell proliferation. The overexpressed FBXO32 gene or the encoded protein thereof can promote the proliferation of skeletal muscle satellite cells, and lays a foundation for improving meat traits of livestock and poultry, improving the feed utilization rate of the livestock and poultry and improving the meat traits of the livestock and poultry.