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1113 results about "Transformation efficiency" patented technology

Transformation efficiency is the efficiency by which cells can take up extracellular DNA and express genes encoded by it. This is based on the competence of the cells. It can be calculated by dividing the number of successful transformants by the amount of DNA used during a transformation procedure. Transformants are cells that have taken up DNA (foreign, artificial or modified) and which can express genes on the introduced DNA.

Amido complex compound and preparation method and usage

The invention discloses an amino complex compound and a preparation method and application thereof. The components of the amino complex compound are MX (NH3)n, wherein, M is one or a plurality of Li, Mg, Al, Ca, Sc, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Sr, Ba, Sn; X is one or a plurality of F, Cl, Br, NO3, SO4; m is determined according to chemical valence, which is less than or equal to 5 and more than or equal to 1; n is determined according to coordination number, which is less than or equal to 15 and more than or equal to 1. The amino complex compound is used as an absorbent of amino after deamination and realizes separation, purification, storage and transporting of ammonia gas through low-temperature ammonia absorption and high-temperature ammonia discharge. The ammonia in circulating gas used in a synthetic ammonia technique of the invention has fast separation speed and the ammonia content entering into a tower can be reduced below 0.1 percent, thus remarkably promoting the ammonia net value and improving the transformation efficiency of the synthetic ammonia technique; furthermore, the invention can be suitable for different ammonia synthesis systems, in particular to a low-pressure ammonia synthesis system and realize the solidification of the ammonia gas during storage and transporting processes; the ammonia storage quantity can reach 40 to 70wt percent with safety and high efficiency.
Owner:ZHEJIANG UNIV

New fracturing process suitable for encrypted development of shale gas well

ActiveCN109977612AIncrease the complexity of the transformationImprove the efficiency of transformationData processing applicationsFluid removalMulti clusterTransformation efficiency
The invention relates to the technical field of horizontal well fracturing, and discloses a new fracturing process suitable for the encrypted development of a shale gas well, which comprises the following steps of (1) collecting the data; (2) analyzing the stress and compressibility; (3) designing the interval of the segment clusters; (4) designing the number of clusters; (5) carrying out the temporary plugging design in the midway; (6) designing the construction displacement; (7) designing a fracturing scale; and (8) designing a sand adding mode. According to the essence of the method, the small-section multi-cluster segmented design of reducing the section cluster spacing is carried out on the target horizontal well by analyzing the single well ground stress characteristics and the compressibility of the shale gas well of encrypted development; meanwhile, the pumping design of the variable-density perforation, midway ball throwing temporary plugging and continuous sand adding is combined, and finally, the effect of improving the single well transformation complexity and the reservoir transformation efficiency is achieved, the volume transformation of the whole horizontal well isachieved, the crack complexity is improved, the transformation strength is improved to a larger extent through improvement of a sand adding mode and a pumping program, and meanwhile the single well construction cost is reduced.
Owner:CHINA PETROLEUM & CHEM CORP +1

Biomass vaporizing combustion coupled type cyclone boiler

InactiveCN101625117ABurn fullyGuaranteed gasification effectChemical industrySolid fuel combustionCycloneCombustion chamber
The invention relates to a biomass vaporizing combustion coupled type cyclone boiler, solving the problems of low thermal efficiency, high tar content in vaporizing gas, easy scorification of a high-temperature heating surface and the like existing in the current biomass boiler, and comprising a jacketed type boiler body and a feeding device thereof, a primary air fan, a secondary air fan, a water circulating pump, an ash slurry pond and a flue water fog deduster. The invention has the technical points that the secondary air fan is connected with the jacket of the boiler body, and an atomizing nozzle is arranged on the inner side of an outer boiler wall; secondary air and water vapour tangential jet holes are distributed on the circumference of an inner boiler wall; one end of a small spiral feeder of the feeding device is connected with the inner cavity of a vaporizing combustion chamber, and the other end is connected with the secondary air fan by a feeding air valve; the vaporizing combustion chamber and a complete combustion chamber are communicated. The invention has reasonable structure design and stable operation and strengthens effects of heat transfer and fuel vaporization, which can increase the transformation efficiency of biomass energy and heat values of biomass fuel vaporizing products, and thus, the biomass fuel is completely and sufficiently burnt, thereby saving energy, increasing the heat efficiency of the boiler, protecting environment and reducing environmental pollution.
Owner:辽宁玺丰新能源科技有限公司

High-frequency isolation single-phase photovoltaic grid-connected system and control method thereof

The invention provides a high-frequency isolation single-phase photovoltaic grid-connected system and a control method thereof. The high-frequency isolation single-phase photovoltaic grid-connected system consists of a photovoltaic array, a buffering capacitor, a high-frequency converter, a high-frequency transformer, an AC / AC converter, a filter and an electric power grid. A high-frequency chain-linked technology is utilized, so the electrical isolation between a photovoltaic power generation system and the electric power grid can be realized, the isolation transformer and the weight and the size of the entire system are reduced, only two-level power conversion is required, the topology is simplified, and the reliability of the entire system and the electric power transformation efficiency are improved. In addition, aiming at the characteristics of the topology and the photovoltaic system, an optimized control algorithm is proposed, so the unfavorable influence of the system from the fluctuation of the voltage outputted by the photovoltaic array can be overcome, the high quality of the electric power outputted by the entire system can be ensured, the distortions is small, the dynamic reaction is fast, the energy can flow in two directions, and reactive compensation can be supplied according to the requirement of the electric power grid.
Owner:SOUTHEAST UNIV

Method for preparing lactic acid by applying double pole film electrodialysis technique

The invention discloses a method for preparing lactic acid with bipolar membrane electrodialysis technology. Lactic acid fermentation liquid passes through an electrodialyzer, which transforms contained lactates into lactic acid. The membrane tank configuration of the adopted electrodialyzer can be of two-cell type including an acid cell and a salt cell divided by an anion membrane or of three-chamber type including an acid cell, a salt cell and a base cell divided by a cation membrane and an anion membrane. For preparation, lactic acid fermentation liquid is transmitted into the salt cell and water is transmitted into the acid cell or the base cell. Under the effect of a circulating pump, the materials flow circularly with a current density of 80-160mA/cm2 at an operation temperature of 25-60 DEG C. The invention can completely supersede the prior calcium lactate acidifying technology without addition of acids for H+ supply and without production of waste acid, salt or base liquid. The transformation efficiency of lactic acid is higher than 98 percent, the recovery of lactic acid is higher than 90 percent and the current efficiency is higher than 70 percent, so the method can reduce environment pollution and lower consumption of chemical raw materials and energy and has remarkable industrial application value and environmental benefit.
Owner:江苏道森生物化学有限公司
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