1120 results about "Transformation efficiency" patented technology

Methods and compositions are provided related to improved plants that are tolerant to more than one herbicide. Particularly, the invention provides plants that are tolerant of glyphosate and are tolerant to at least one ALS inhibitor, and methods of use thereof. The glyphosate / ALS inhibitor-tolerant plants comprise a polynucleotide that encodes a polypeptide that confers tolerance to glyphosate and a polynucleotide that encodes an ALS inhibitor-tolerant polypeptide. In specific embodiments, a plant of the invention expresses a GAT polypeptide and an HRA polypeptide. Methods to control weeds, improve plant yield, and increase transformation efficiencies are provided.

The invention relates to the field of biology breeding and in particular relates to an agrobacterium-mediated ustilago esculenta (Ustilago esculenta) transformant strain as well as a preparation method and application thereof. According to the agrobacterium-mediated ustilago esculenta transformant strain, an agrobacterium infected receptor of the transformant strain refers to ustilago esculenta (Ustilago esculenta). The method for preparing the agrobacterium-mediated ustilago esculenta transformant strain comprises the following steps: (1) preparing a fungal expression vector; (2) preparing agrobacterium competence; (3) performing electrotransformation on the agrobacterium; and (4) performing agrobacterium-mediated transformation, thereby obtaining the transformant. A complex protoplast is not needed, the receptor source is simple, and spores and hypha of the fungi can be directly used for transformation; and the transformation efficiency is high. In addition, the obtained transformant is large in single-copy ratio, and marker genes are conveniently obtained.

Reduced genome strains of E. coli MGl 655 are described. In various embodiments, the strains have one or more of equal or improved growth rate, transformation efficiency, protein expression, DNA production, DNA yield and / or DNA quality compared to the parental strain and commercially available strains.

The invention discloses an amino complex compound and a preparation method and application thereof. The components of the amino complex compound are MX (NH3)n, wherein, M is one or a plurality of Li, Mg, Al, Ca, Sc, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Sr, Ba, Sn; X is one or a plurality of F, Cl, Br, NO3, SO4; m is determined according to chemical valence, which is less than or equal to 5 and more than or equal to 1; n is determined according to coordination number, which is less than or equal to 15 and more than or equal to 1. The amino complex compound is used as an absorbent of amino after deamination and realizes separation, purification, storage and transporting of ammonia gas through low-temperature ammonia absorption and high-temperature ammonia discharge. The ammonia in circulating gas used in a synthetic ammonia technique of the invention has fast separation speed and the ammonia content entering into a tower can be reduced below 0.1 percent, thus remarkably promoting the ammonia net value and improving the transformation efficiency of the synthetic ammonia technique; furthermore, the invention can be suitable for different ammonia synthesis systems, in particular to a low-pressure ammonia synthesis system and realize the solidification of the ammonia gas during storage and transporting processes; the ammonia storage quantity can reach 40 to 70wt percent with safety and high efficiency.

The invention relates to a phosphorus gettering process of silicon chips in the manufacture of solar cells, which comprises the following steps: putting sueded silicon chips into a diffusion furnace for pre-deposition, removing a phosphorosilicate glass layer after diffusion, soaking the silicon chips through distributed processing in a hydrofluoric acid solution, and removing oxide layers from the surfaces of the silicon chips; then putting the silicon chips after washing into the diffusion furnace for secondary diffusion processing, taking the silicon chips out from the diffusion furnace after the processing of the secondary diffusion working procedure, cooling the silicon chips to room temperature, and measuring the square resistance of the silicon chips. The invention can effectively decrease heavily doped 'dead layers' and greatly prolong the average minority carrier lifetime of the silicon chips; after the processes including etching, PECVD, silk screen sintering and the like arefinished according to the normal process of a cell chip, the average transformation efficiency of the made cell chip is further improved, and the cell chip has better electrical performance parameters.

A process for producing lyophilized competent cells wherein competent cells are that can be stored or shipped as freeze-dried cells at temperatures between 0° C. and 8° C. and remain suitable for cloning genes or DNA fragments. The process includes culturing cells, rending the cells competent, and lyophilizing the cells. Once lyophilized, the cells can be stored or shipped as freeze-dried cells. The lyophilized cells are prepared for transformation protocols by being re-hydrated in a solution of dimethyl sulfoxide. Once re-hydrated, the transformation efficiency of the competent cells is at least 5x105 transformations per microgram of DNA.

The invention relates to the technical field of horizontal well fracturing, and discloses a new fracturing process suitable for the encrypted development of a shale gas well, which comprises the following steps of (1) collecting the data; (2) analyzing the stress and compressibility; (3) designing the interval of the segment clusters; (4) designing the number of clusters; (5) carrying out the temporary plugging design in the midway; (6) designing the construction displacement; (7) designing a fracturing scale; and (8) designing a sand adding mode. According to the essence of the method, the small-section multi-cluster segmented design of reducing the section cluster spacing is carried out on the target horizontal well by analyzing the single well ground stress characteristics and the compressibility of the shale gas well of encrypted development; meanwhile, the pumping design of the variable-density perforation, midway ball throwing temporary plugging and continuous sand adding is combined, and finally, the effect of improving the single well transformation complexity and the reservoir transformation efficiency is achieved, the volume transformation of the whole horizontal well isachieved, the crack complexity is improved, the transformation strength is improved to a larger extent through improvement of a sand adding mode and a pumping program, and meanwhile the single well construction cost is reduced.

Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.

The present invention provides a formula of high-yield flammulina velutipes culture medium and a producing technique thereof. The invention relates to the technical filed of biological strain, and specially to the formula of high-yield flammulina velutipes culture medium and producing technique thereof. The corncob is used as main ingredient for replacing fir sawdust. The invention adopts 40-45% of corncob, 10-12% of bagasse, 25-30% of rice bran, 8-10% of bran, 4-6% of soybean hull and 2-3% of corn flour as the raw materials of flammulina velutipes culture medium. The method comprises the techniques of dry mixing, wet mixing, etc. The pH value is controlled between 7.0 and 7.5. The cultured flammulina velutipes has the advantages of strong activity of mycelium, high production volume, ordered mushroom generation, excellent commodity valve, ordered growing of sporphore, uniform magnitude of bonnet, white color, and no infection of foreign fungus. The unit production obtains 286-293grams / bottle. The dry object of each bottle is 260grams. The biological transformation efficiency reaches up to 110-113% and is increased for 30-40% compared with the common formula. The economic benefit is excellent.

This invention relates to a synthesis method of fischer-tropsch. Its preparation includes (a) Synthesis gas material enters into the first fischer-tropsch synthesis reactor to conduct fischer-tropsch synthesis reaction under the action of accelerant, (b) Segregating the first grade reaction product and letting parts of end gas return to the first fischer-tropsch reactor for recycling, and then letting C1-C4 alkanes containing in other surplus end gas to transform into synthetic gas.(c) Mixing the end gas transformed at step (b) and the second fischer-tropsch synthesis reaction circulation gas, and then entering the second fischer-tropsch synthesis reactor to conduct fischer-tropsch synthesis reaction under accelerant. (d) Segregating the second fischer-tropsch synthesis reaction product, and most of the gas returning to the second fischer-tropsch synthesis reactor for circulating reaction, and then evacuating other end gas. The transformation efficiency of CO+H2 is higher than 96% and the yield of methyl hydride is lower than 3%, and the yield of unit space-time of accelerant reaches 185 g / kg*h.

The invention relates to a biomass vaporizing combustion coupled type cyclone boiler, solving the problems of low thermal efficiency, high tar content in vaporizing gas, easy scorification of a high-temperature heating surface and the like existing in the current biomass boiler, and comprising a jacketed type boiler body and a feeding device thereof, a primary air fan, a secondary air fan, a water circulating pump, an ash slurry pond and a flue water fog deduster. The invention has the technical points that the secondary air fan is connected with the jacket of the boiler body, and an atomizing nozzle is arranged on the inner side of an outer boiler wall; secondary air and water vapour tangential jet holes are distributed on the circumference of an inner boiler wall; one end of a small spiral feeder of the feeding device is connected with the inner cavity of a vaporizing combustion chamber, and the other end is connected with the secondary air fan by a feeding air valve; the vaporizing combustion chamber and a complete combustion chamber are communicated. The invention has reasonable structure design and stable operation and strengthens effects of heat transfer and fuel vaporization, which can increase the transformation efficiency of biomass energy and heat values of biomass fuel vaporizing products, and thus, the biomass fuel is completely and sufficiently burnt, thereby saving energy, increasing the heat efficiency of the boiler, protecting environment and reducing environmental pollution.

The invention belongs to the field of biotechnology, and in particular relates to a mutant of diketoreductase (DKR) that can be used as a catalyst for chiral alcohol synthesis; the invention discloses a mutant of diketoreductase, the diketoreductase The amino acid sequence of the mutant has more than 80% homology with any amino acid sequence shown in SEQ ID NO.1-SEQ ID NO.20. Compared with the wild-type double carbonyl reductase, the double carbonyl reductase mutant of the present invention has higher transformation efficiency and higher stereoselectivity.

Methods and compositions are provided related to improved plants that are tolerant to more than one herbicide. Particularly, the invention provides plants that are tolerant of glyphosate and are tolerant to at least one ALS inhibitor, and methods of use thereof. The glyphosate / ALS inhibitor-tolerant plants comprise a polynucleotide that encodes a polypeptide that confers tolerance to glyphosate and a polynucleotide that encodes an ALS inhibitor-tolerant polypeptide. In specific embodiments, a plant of the invention expresses a GAT polypeptide and an HRA polypeptide. Methods to control weeds, improve plant yield, and increase transformation efficiencies are provided.

The invention discloses a CdZnTe thin film solar energy cell comprising: a glass substrate where a transparent conductive oxide front electrode layer, a n type CdS window layer, a p type Cd<1-x>ZnxTe absorption layer, a p type ZnTe:Cu back contact electrode layer are deposited. The advantages of the invention lie in: the p type Cd<1-x>ZnxTe absorption layer is an absorption layer gradually changes with gap that expand the responding wave section of the solar energy cell by gradual changes with gap. When combined with the p type ZnTe:Cu back contact electrode layer, the p type Cd<1-x>ZnxTe absorption layer has a lower work function characteristic, which effectively solves the ohm contact problem to improve photoelectric transformation efficiency and stability of the cell.

The invention discloses an electrode structure for improving a crystal silicon solar battery photoelectric transformation efficiency characterized by arranging parallely set gate lines on a light interception face of the crystal silicon solar battery substrate, and arranging at least two solder joints between each gate line and the crystal solar battery substrate light interception face, wherein the gate lines are conductive gate lines. The invention cancels split-gate lines in a background technique, uses the solder points between the metal gate lines and the crystal solar battery substrate to gather light currents of the crystal silicon solar battery light interception face and guides the currents gathered by the solder points by using the metal gate lines. Because said structure, the invention not only reduces a lightproof area of the light interception face gate line of the crystal silicon solar battery, but also reduces a series resistance of the crystal silicon solar battery, thereby improving the conversion efficiency of the crystal silicon solar battery and facilitating the interconnect of multi-block single batteries.

Methods and compositions for improved bacterial-mediated plant transformation are provided. The methods generally allow plant transformation with reduced vector backbone integration and a high frequency of low-copy transformation events. Vectors for achieving these results are described, as are methods for their use.

The invention discloses a preparation method of a transparency film used for increasing photoelectric conversion efficiency of a solar photovoltaic cell. The transparency film used for increasing photoelectric conversion efficiency of the solar photovoltaic cell comprises: (1) at least one down conversion agent A; (2) at least one up conversion agent B; (3) at least one up and down conversion agent C; (4) at least three visible light arousing long afterglow light conversion agent D, E, F; (5) a matrix resin (polyethylene, polystyrene or polyvinyl chloride); (6) an ageing resistant consistent with above materials (1), (2), (3), (4) and (5). The transparency film used for increasing photoelectric conversion efficiency of the solar photovoltaic cell is provided with the characteristics of optical conversion and noctilucence, and is lasting and stable, can cause the common photovoltaic cell to make use of solar for longer time and more effectively, and mostly can improve the photoelectric conversion efficiency of the common photovoltaic cell more than 30%, namely can cause the photoelectric conversion efficiency of the common photovoltaic cell to achieve more than 22%.

The invention discloses an anti-reflecting film applied to a metallurgical silicon solar cell, which is composed of two layers of films, wherein the first layer film is arranged on the surface of a silicon slice substrate of an ultra-purity metallurgical-grade polysilicon solar battery, the thickness of the first layer film is 35-50nm, and the refracting index is 2.25-2.35; the second layer film is arranged on the surface of the first layer film, the thickness of the second layer film is 40-55nm, and the refracting index is 1.95-2.05; components of the two films are both silicon nitride; the comprehensive film thickness of the two layers of films is 82-89nm, and the comprehensive refracting index is 2.03-2.12. The anti-reflecting film can obviously reduce the refracting of the surface of the battery to light, and improves the photoelectric transformation efficiency of the ultra-purity metallurgical-grade polysilicon solar cell.

The invention provides a high-frequency isolation single-phase photovoltaic grid-connected system and a control method thereof. The high-frequency isolation single-phase photovoltaic grid-connected system consists of a photovoltaic array, a buffering capacitor, a high-frequency converter, a high-frequency transformer, an AC / AC converter, a filter and an electric power grid. A high-frequency chain-linked technology is utilized, so the electrical isolation between a photovoltaic power generation system and the electric power grid can be realized, the isolation transformer and the weight and the size of the entire system are reduced, only two-level power conversion is required, the topology is simplified, and the reliability of the entire system and the electric power transformation efficiency are improved. In addition, aiming at the characteristics of the topology and the photovoltaic system, an optimized control algorithm is proposed, so the unfavorable influence of the system from the fluctuation of the voltage outputted by the photovoltaic array can be overcome, the high quality of the electric power outputted by the entire system can be ensured, the distortions is small, the dynamic reaction is fast, the energy can flow in two directions, and reactive compensation can be supplied according to the requirement of the electric power grid.

The invention relates to a method for manufacturing a selective emitter junction of a multricrytalline silicon solar cell. The method comprises a step that slicon nitride is used as a masking film to perform dual diffusion and shallow diffusion so as to form dual diffusion region and shallow diffusion region on the surface of a silicon chip. Favorable to the improvement of the collection of photon-generated carrier, particularly the improvement of the collection rate of shortwave photon-generated carrier, the method can improve the open circuit voltage Voc, short-circuit current Isc and filling factor F.F. of the solar cell so as to make the cells gain high photoelectric transformation efficiency. The advantage results from the different doping concentrations and the different diffusion depth formed in the different regions of the solar cell.

Disclosed a high-accurate self-adapting ultrasonic energy converter driving power, comprises a processing circuit based on DSP, a multi-path high-speed / DA converter circuit, a multi-path high-speed A / D converter circuit, a digital-analogue phase-lock loop, a accumulator circuit, a high-accurate broad band voltage-controlled oscillator circuit, a phase detector circuit, a power amplifier circuit, a output current detecting circuit, a micro-current detecting circuit, a output voltage detecting circuit, and a interface circuit. The invention has the ability to control program with self-adapting servo-algorism, which can trace the working condition of energy converter in time, and process the feedback information to compensate and modify the energy of ultrasonic energy converter, which can meet the energy converters with different types without changing parameters of hardware, to realize best condition of transformation efficiency of ultrasonic energy converter.

The invention discloses a method for agrobacterium tumefaciens-mediated genetic transformation of sugarcane. The method comprises the steps: leading agrobacterium tumefaciens liquor carrying plant expression vectors to infect embryogenic callus of the sugarcane; and selecting and proliferating resistant calli to induce the resistant calli to obtain the resistant buds of the sugarcane, wherein matrix attachment region sequences are connected at two sides of a gene expression box of the plant expression vector. According to the method, MARs (metal artifact reduction) sequences are built at two sides of the gene expression box of the plant expression vector, thus greatly improving the exogenous gene transformation efficiency and seedling rate of the sugarcane, and being simple in process flow and low in cost.

The present invention discloses that a bacterium having a genome that is genetically engineered to be at least 10% smaller than the genome of its native parent strain has better transformation competence. Specific E. coli strains, having significantly reduced genome sizes, are disclosed which are highly transformation competent. A medium and methodology is taught which enables transformation efficiencies to be increased further.

The invention discloses a single-stage bidirectional isolation AC-DC converter, and belongs to the field of an electronic power electronic converter. The structures of three converters are each composed of single-phase AC sources, DC sources, Boost inductors, phase shift inductors, bus capacitors, transformers, primary power switch tubes, secondary power switch tubes and the like. The Boost inductors are connected to leg midpoints such that the integration of a bidirectional Boost PFC converter with a bridge type bidirectional converter is realized, through single-stage power transformation, two functions of PFC and isolation DC-DC transformation can be realized, and bidirectional flowing of energy is realized. The single-stage bidirectional isolation AC-DC converter, has the advantages of small power loss, high transformation efficiency, small switching loss, capability of realizing the bidirectional flowing of the energy, and the like, thereby being especially suitable for V2G and power network-storage battery energy storage systems.

The invention discloses a method for performing transient expression by introducing a foreign gene into a poplar bioplast. The method comprises the following steps of: introducing a transient expression vector containing a foreign gene into a poplar bioplast by using PEG (Polyethylene Glycol)-Ca<2+>; culturing; and expressing the foreign gene in the bioplast. A poplar bioplast transient expression system constructed with the method is easy to operate and realize, a place with broad prospects is opened up for the research of functional genes of a poplar, and the transformation efficiency of the method can be up to 70 percent.

The invention discloses a method for preparing lactic acid with bipolar membrane electrodialysis technology. Lactic acid fermentation liquid passes through an electrodialyzer, which transforms contained lactates into lactic acid. The membrane tank configuration of the adopted electrodialyzer can be of two-cell type including an acid cell and a salt cell divided by an anion membrane or of three-chamber type including an acid cell, a salt cell and a base cell divided by a cation membrane and an anion membrane. For preparation, lactic acid fermentation liquid is transmitted into the salt cell and water is transmitted into the acid cell or the base cell. Under the effect of a circulating pump, the materials flow circularly with a current density of 80-160mA / cm2 at an operation temperature of 25-60 DEG C. The invention can completely supersede the prior calcium lactate acidifying technology without addition of acids for H+ supply and without production of waste acid, salt or base liquid. The transformation efficiency of lactic acid is higher than 98 percent, the recovery of lactic acid is higher than 90 percent and the current efficiency is higher than 70 percent, so the method can reduce environment pollution and lower consumption of chemical raw materials and energy and has remarkable industrial application value and environmental benefit.

Methods and compositions to increase Agrobacterium transformation efficiency (frequencies) in both dicot and monocot host plants include adding histones to the host plant at most transiently, and using histones and L-cysteine at certain stages in monocot transformation.