1096 results about "Reference genome" patented technology

The invention discloses an SNP molecular marker related to the conception rate of young cows, a detection product and application of the SNP molecular marker, and belongs to the technical field of dairy cow breeding markers. The SNP molecular marker is chr6: g. 103355581Cgt, and the SNP molecular marker is a molecular marker. The method comprises the following steps of: taking a bovine reference genome version UMD3.1 as a standard for a coordinate position, numbering Assessment in NCBI (National Center of Biotechnology Information) as GCF000003055.6, and numbering a chromosome as AC 000163.1; the young cows are cows which are 12-24 months old and do not lay calves. By detecting the genotype of the SNP site, the conception rate condition can be effectively judged, parent individuals with the higher young cow conception rate can be screened, early selection of genotype individuals with the high young cow conception rate is achieved, the frequency of the genotype with the high young cow conception rate in a dairy cow group is increased, and the reproduction rate of the dairy cow group is increased; the service life of cows is prolonged, the treatment cost is saved, the breeding cost is reduced, and the pasture income is increased.

The invention belongs to the field of molecular biological identification, and particularly relates to a specific SNP (Single Nucleotide Polymorphism) site combination for identifying a Wuzhishan pig variety and application. The specific SNP site combination for identifying the Wuzhishan pig variety comprises 77 SNP sites, and the physical positions of the SNP sites are determined by sequence comparison based on a pig reference genome Sscrofa11.1. The specific SNP site combination is screened based on a method of combining whole genome association analysis with selection signal analysis so as to ensure the accuracy of site selection. The selected specific SNP site combination can rapidly realize accurate identification of the Wuzhishan pig variety on the gene level, and has significant application value in the aspects of genetic resource accurate protection and variety utilization of Hainan Wuzhishan pigs.

The invention discloses a KASP molecular marker for identifying soybean hundred-grain weight and application thereof, and relates to the technical field of biology and application of an SNP locus, the SNP locus takes a soybean Wm82. A2. V1 genome sequence as a reference genome, the SNP is the 16321243th SNP on a soybean 11 # chromosome, and the SNP is the 16321243th SNP on the soybean 11 # chromosome. Corresponding to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, when the locus is CC homozygosis, the corresponding genotype is A; when the locus is TT homozygous, the corresponding genotype is B; according to the application, soybeans with different hundred-grain weights are screened or screened in an auxiliary mode, and the different hundred-grain weights of the soybeans are as follows: the homozygous soybeans with the genotype A are higher than or candidate to be higher than the homozygous soybeans with the genotype B. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding progeny materials with high hundred-grain weight.

The invention relates to the technical field of crop genetic breeding and plant molecular design breeding, in particular to a pakchoi whole genome liquid phase chip and application. The pakchoi breeding gene chip disclosed by the invention comprises a target capture probe which is designed by taking 1000 core SNP (Single Nucleotide Polymorphism) molecular markers positioned on 40KSNP sites on a pakchoi reference genome version V4.0 as templates. The pakchoi whole genome liquid phase chip provided by the invention comprises a 40K site targeted capture probe mixed solution and a hybridization capture reagent. The pakchoi 40KSNP targeted capture probe can rapidly and effectively track genetic materials of pakchoi, and is suitable for different application scenes such as accurate identification of pakchoi germplasm resources, genetic relationship analysis between materials, variety authenticity identification, QTL gene positioning and mining, molecular marker-assisted breeding, variety improvement and whole genome selective breeding application. Therefore, the method has good application prospect and important social value.

The invention relates to the technical field of molecular biology, and particularly provides an InDel molecular marker related to the size of a lychee kernel and application of the InDel molecular marker. The InDel molecular marker is located at a site 25610680 of a fifth chromosome of a reference genome GeneBank: GCA019925255.1 of the litchi, and the InDel molecular marker is used for detecting the DNA of the litchi. The genotype of the marker in a pyrokaryotic litchi material is homozygous' GAAAAA / GAAAAA ', and the genotype of the marker in a macrokaryotic litchi material is heterozygous' G---- / GAAAAA 'or homozygous' G---- / G----'. The invention further provides a primer group for detecting the molecular marker, an effective tool is provided for molecular marker-assisted breeding of the size of the lychee kernel, and the method is expected to assist in shortening the breeding period, improving the breeding efficiency and promoting the improvement work of the lychee variety.

The invention discloses a tetraploid crassostrea gigas parent-child relationship identification method based on SNP sites, and belongs to the technical field of tetraploid crassostrea gigas molecular assisted breeding. The method for identifying the parent-child relationship of tetraploid crassostrea gigas comprises the following steps: (1) sequencing adductor muscles of tetraploid crassostrea gigas to be detected; (2) comparing the reference genome of the crassostrea gigas to obtain the genotype of the specified SNP site of the tetraploid crassostrea gigas; (3) calculating IBS and LOD of the to-be-detected parents and the to-be-detected offspring according to an SNP typing result; and (4) identifying the parent-child relationship between the to-be-detected parent and the filial generation by combining IBS and LOD. The SNP loci have the advantages that 1000 SNP loci for identifying the parent-child relationship of the tetraploid crassostrea gigas are disclosed for the first time, data support is provided for identifying the parent-child relationship of the tetraploid crassostrea gigas, a foundation is laid for developing a liquid phase chip for identifying the parent-child relationship of the tetraploid crassostrea gigas, and the SNP loci have good application prospects.

The invention belongs to the technical field of pepper planting, and discloses a pepper multi-branch gene CaBr1 linked KASP molecular marker as well as a primer, a kit and application thereof, the KASP molecular marker takes pepper S8 (zhangshuang) as a reference genome, and the single nucleotide polymorphism at the 103175415th basic group on the No.8 chromosome is subjected to basic group G-to-A replacement. A chromosome region closely linked with multiple branches of the pepper is obtained by using a BSA (Bovine Serum Albumin) population positioning method, a molecular marker is developed in a candidate region to determine a candidate gene CaBr1, a KASP molecular marker is screened according to base mutation of the candidate gene, and the marker is used for carrying out genotype identification on 90 randomly sampled single plants of an F2 population, and the coincidence rate reaches 100%. The result not only contributes to pepper branch phenotype identification and assistant breeding, but also lays a foundation for map-based cloning of the branch gene and analysis of a molecular mechanism of pepper plant type regulation.

The invention relates to a molecular marker closely linked with a pepper fruit length regulation gene CaFL1 and application of the molecular marker. The molecular marker is insertion / deletion of three basic groups GAC at 223149123-223149125th positions of chromosome 10 of a pepper genome, and the position of the molecular marker in the genome is determined according to a Qieme reference genome. Specifically, when the genotype of the molecular marker is InGAC: InGAC, the pepper has a long fruit phenotype, when the genotype is InGAC: DelGAC, the pepper has an intermediate phenotype, and when the genotype is DelGAC: DelGAC, the pepper has a short fruit phenotype. Through verification, the accuracy rate of the molecular marker reaches 100%, early, massive and accurate prediction and directional improvement of the pepper fruit length can be realized, and a new way is provided for screening and identifying the pepper fruit length and breeding the pepper fruit length.

The invention relates to the technical field of crop molecular genetic markers, and discloses an InDel molecular marker of avena nuda and application thereof.The molecular marker is a group of 314bp deletion variation and 2bp insertion variation in the 610439484-610440026 interval of the 5C chromosome position of a reference genome of an avena nuda variety OT3098, the nucleotide sequence of the deletion variation is shown as SEQ ID NO.1, the nucleotide sequence of the insertion variation is shown as SEQ ID NO.2, and the nucleotide sequence of the insertion variation is shown as SEQ ID NO.3. The nucleotide sequence of the insertion variation is 5 '-AA-3'. The molecular marker provided by the invention can realize stable detection in agarose gel electrophoresis by combining a specific primer pair (SEQ ID NO.2-3) with an optimized touchdown PCR (Polymerase Chain Reaction) program, has the advantage of being not influenced by a tissue part and a growth period, and provides an efficient and reliable technical means for oat germplasm identification, seed purity detection and molecular breeding.

The invention discloses a KASP molecular marker for identifying soybean protein content and application, and relates to the technical field of biology and application of an SNP locus, the SNP locus takes a soybean Wm82.a2.v1 genome sequence as a reference genome, is the 42148495th SNP on a soybean chromosome 9 and corresponds to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, and when the locus is TT homozygosis, the corresponding genotype is A; when the site is AA homozygous, the corresponding genotype is B; according to the application, different protein contents of soybeans are screened or screened in an auxiliary mode, and the different protein contents of the soybeans are as follows: the homozygous soybeans of the genotype A are higher than or candidate to be higher than the homozygous soybeans of the genotype B. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding offspring materials with high protein content.

The invention discloses a KASP molecular marker for identifying the soybean oil content and application, and relates to the technical field of biology and application of an SNP locus, the SNP locus takes a soybean Wm82. A2. V1 genome sequence as a reference genome, the SNP is the 6562032th SNP on a soybean 20 # chromosome, and the SNP is the 6562032th SNP on the soybean 20 # chromosome. Corresponding to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, when the site is GG homozygosis, the corresponding genotype is A; when the site is AA homozygous, the corresponding genotype is B; according to the application, soybeans with different oil contents are screened or screened in an auxiliary mode, and the different oil contents of the soybeans are as follows: the soybean with the homozygous genotype A is higher than the soybean with the homozygous genotype B or the soybean with the homozygous genotype B in a candidate mode. The invention has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding progeny materials with high oil content.

The invention provides an ecDNA structure prediction method based on scATAC-seq, a method for identifying cells carrying ecDNA and a medium, and relates to the technical field of biological information. According to the ecDNA structure prediction method based on scATAC-seq, inconsistent readings of ecDNA breakpoint sources are extracted through comparison with a reference genome, enriched peak regions are called in sequence and converted into a graph, a breakpoint graph is combined with a Bayesian model, and accurate prediction of structural information such as ecDNA breakpoints, ecDNA regions and connection modes is achieved. The invention also provides a method for identifying cells carrying the ecDNA, and the method is used for accurately acquiring the cells carrying the ecDNA through standardization treatment, formation of a gene activity matrix and non-single-peak test on the ecDNA structure based on the scATAC-seq. According to the present invention, the ecDNA structure can be predicted, the cells carrying the ecDNA can be accurately identified, and the method can be used for the accurate analysis and research of the tumor heterogeneity, evolution and drug resistance process of the ecDNA.

The invention belongs to the field of molecular biology, and discloses acquisition of a waterlogging-resistant major QTL qWT1.1 in a corn seedling stage and development and application of a molecular marker primer of the waterlogging-resistant major QTL qWT1.1. The invention provides a major QTL qWT1.1 for controlling the waterlogging survival rate in the corn seedling stage, the major QTL qWT1.1 is located at the fifth version physical position of a reference genome in the first staining of the corn, the fifth version physical position of the reference genome is Chr1: 25904794-27039204, the major QTL site is used for controlling waterlogging-resistant phenotypic variation, an excellent haplotype SNP marker closely linked with the major QTL site is located at the 27039148th basic group of a first chromosome of a reference genome of the corn B73, and the excellent haplotype SNP marker is located at the 27039148th basic group of a second chromosome of the reference genome of the corn B73. The explained waterlogging survival rate phenotypic contribution rate is 10.03%, the PARMS marker designed by the SNP is used for detecting a corn inbred line group, the operation is simple and convenient, the typing is clear, the good selection effect on characters such as root length, root number, plant height, branch number and yield under the stress of corn waterlogging is realized, and the application prospect is wide. The molecular marker can be used for molecular marker-assisted selective breeding of the waterlogging resistance character in the corn seedling stage.

The invention relates to application of a molecular marker related to daily gain of pigs at the stage of 30kg-120kg. The invention discloses a method for identifying the daily gain of pigs at the stage of 30 kg to 120 kg and a breeding method for breeding the pigs with the daily gain at the stage of 30 kg to 120 kg. According to the method, by detecting the genotype of a to-be-detected pig at the 161610871th site of a chromosome 1 in a pig reference genome Susscrofa.Sscrofa11.1, the daily gain of the pig at the stage of 30 kg to 120 kg is identified, the pig with the TT genotype is higher than the pig with the CT genotype, and the pig with the CT genotype is higher than the pig with the CC genotype. In actual breeding work, a to-be-detected pig with the genotype of the C161610871T site being TT can be selected as a parent for breeding, and the method has important significance on selection of pigs with high feed intake and high feed utilization efficiency.

The invention belongs to the technical field of molecular markers, and discloses a molecular marker located in No.18 chromosome and related to the salt tolerance of soybean seeds in the germination period and application of the molecular marker. According to the present invention, the salt tolerance index of the soybean seed germination stage is determined, the stable and reliable molecular marker GmChr1856968562 closely linked with the salt tolerance ability of the seed germination stage is obtained through GWAS correlation analysis, the molecular marker is located at the 56968562 site on the No.18 chromosome of the soybean reference genome Glycine max Wm82. A2. V1, and the marker for KASP detection is developed based on the site, the invention also designs a primer group for amplifying the KASP marker. The invention further provides a method for rapidly identifying the salt tolerance of the soybean seeds in the germination period by using the primer group of the molecular marker, and the method is simple, convenient and rapid, accurate in identification result and good in popularization and application prospect.

The invention relates to the technical field of molecular detection, and particularly discloses a liquid chip for identifying opisthopapus taihangensis and Hebei province as well as a preparation method and application of the liquid chip. Genotyping sites of the liquid chip comprise 1140 SNP sites; the physical positions of the 1140 SNP loci are determined based on comparison of a whole genome sequence of a bovine reference genome, and the version number of the whole genome sequence of the bovine reference genome is ARSUCD 1.2; wherein the physical position information of the 1140 SNP loci is as shown in a table 1. The liquid chip can realize genetic typing of the opisthopapus taihangensis and the Hebei province cattle, has the advantage of high detection rate, and can obviously cluster the opisthopapus taihangensis and the Hebei province cattle into two classes when clustering analysis is carried out on the opisthopapus taihangensis and the Hebei province cattle, so that a marker combination of the liquid chip can represent whole genome re-sequencing genes, assists in distinguishing the opisthopapus taihangensis and the Hebei province cattle, and is high in accuracy and high in accuracy. Therefore, the variety identification of the opisthopapus taihangensis and the Hebei Hebei is realized.

The invention relates to the technical field of bioinformatics data processing, in particular to a genome sequence compression method and system. The method comprises the following steps: acquiring genome sequencing data; comparing the sequencing data with a reference genome to determine a difference site; differentiating the difference sites as sequencing errors or real variations through a time sequence difference neural network model; performing differential compression coding according to an identification result; a friendly variation detection format is constructed, and rapid variation query is supported through a multi-level index structure and a variation metadata table. According to the method provided by the invention, the sequencing error and the real variation can be accurately distinguished through the time sequence differential neural network model, and important biological variation information is protected while the compression efficiency is improved by adopting the differential compression coding strategy.

The invention discloses a muskmelon fruit length molecular marker and application thereof. According to the method, two methods of BSA-seq and GPS-seq are utilized to perform preliminary positioning on the muskmelon fruit length related QTL, a reference genome Mel (DHL92) gene 3.6. 1 and a re-sequencing result are utilized to perform comparative analysis, two algorithms of SNP-index and InDel-index are comprehensively utilized to perform correlation analysis, the results of the two analysis methods are respectively counted, and the results of the two analysis methods are compared with those of the QTL. The larger segment is used as a candidate region associated with the length of the melon fruit, KASP06-3880 and KASP06-3606 are obtained, and the KASP06-3880 is located at the 34305438th basic group of the No.6 chromosome; the KASP06-3606 is located at the base at the 18936169th site of the chromosome 11; the two KASP markers KASP06-3880 and KASP11-3606 are subjected to genotype detection in extreme materials of F2 and RIL populations, and the two KASP markers determine the length of the muskmelon fruits, so that a new way is provided for breeding of the muskmelon fruits with different lengths.

The invention discloses an InDel marker closely linked with the sweet-glutinous homograin character of corn ears, a corn B73-V4 genome is used as a reference genome, the marker is a <-> / insertion sequence of 73 bp located at the position of 12126328 bp of a No.9 chromosome, and the nucleotide sequence of the insertion sequence is as shown in SEQ ID NO.1. The InDel marker closely linked with the sweet-glutinous homograin character of the corn ears has the advantages that the InDel marker closely linked with the sweet-glutinous homograin character of the corn ears is obtained; the InDel marker provided by the invention can be used for rapidly screening out a corn material containing a sweet-waxy homograin gene in the early growth stage of corn, and can also be used for molecular marker-assisted breeding of fresh corn. By adopting the InDel marker and the screening method provided by the invention, the screening accuracy is high, and by using the marker and the screening method, the production cost is saved, and the breeding efficiency is improved.

The invention discloses an indel molecular marker for identifying color and luster of citrus peel in a seedling stage and application of the indel molecular marker. The corresponding sites of the indel molecular marker of the citrus are positioned in chr7: 18, 398, 798-18, 399 and 289 intervals of a chromosome 7 of an SWO3.0 reference genome; the genome DNA of the citrus seedling stage plant is used as a template, the Indel molecular marker is amplified through PCR, the citrus seedling stage plant can be divided into a homozygous insertion type, a homozygous deletion type and a heterozygous type according to a PCR amplification result, and the three types are associated with the color of the citrus fruit for identifying the color of the citrus peel, so that the early screening of the seedling stage target traits of the citrus breeding population is realized, and the early screening of the seedling stage target traits of the citrus breeding population is realized. The breeding period is remarkably shortened, the breeding cost is reduced, and important technical support is provided for citrus fruit color improvement breeding.

The invention belongs to the technical field of molecular biology and genetic breeding, and discloses a molecular marker closely linked with soybean plant height QTL qPH1.01 and application of the molecular marker. According to the present invention, the major QTL qPH1.01 for regulating corn plant height variation is identified by using linkage analysis, the significantly associated SNP is located at the 27th, 318th and 904th bases of the first chromosome of the reference genome Glycinemaxv2.1, 6.28-11.14% phenotypic variation can be explained in the single environment, the PARMS marker developed by using the SNP is used to detect 96 soybean varieties, the typing is clear, the operation is simple and convenient, the method is suitable for soybean plant polymer breeding, and the application prospect is broad. And a new high-yield soybean variety with proper plant height and lodging resistance is cultivated.

The invention provides a beet genetic diversity analysis method based on whole genome re-sequencing. The method comprises the steps of PacBio HiFi library construction, computer sequencing, data information analysis, assembly quality evaluation and Hi-C auxiliary genome assembly. The invention further provides application, and the high-continuity beet reference genome is used for analyzing beet intraspecific genetic diversity and developing high-density molecular markers. According to the method, the high precision of HiFi, the super-long read length of Nanopore and Hi-C space interaction data are combined for the first time, the problems of repeated sequence and complex SV analysis are solved, and all-round breakthrough of genome continuity, annotation integrity, genetic diversity coverage and epigenetic integration is achieved.

The present disclosure relates to methods for detecting unique genetic signatures derived from markers such as, for example, mutations, somatic or germ-line, in nucleic acids obtained from biological samples. The sensitivity of the methods provides for detection of mutations associated with a disease, e.g., cancer mutations, or with inherited disease, e.g., an autosomal recessive disease, in a noninvasive manner at ultra-low proportions of sequences carrying mutations to sequences carrying normal, e.g., non-cancer sequences, or a reference sequence, e.g., a human reference genome.

The invention belongs to the technical field of molecular biology and genetic breeding, and discloses a molecular marker of a locus qSGC.A8-3 remarkably associated with glucosinolate in brassica napus seeds and application of the molecular marker. A locus qSGC.A8-3 remarkably related to the glucosinolate content of the rape seeds and a peak SNP marker Bn-A08-p12638473 of the locus qSGC.A8-3 are located at 10th, 557th and 348th basic groups of a reference genome A08 chromosome of the rape DarmorV4.1, the average additive effect is 9.6 mu mol / g, and 7.5% of phenotypic variance can be explained. When the PARMS marker designed by the SNP is used for detecting rape germplasm resources, the PARMS marker is simple and convenient to operate and clear in typing, and the average thioglycoside content of AA genotype material seeds is higher than that of GG genotype material seeds, so that the PARMS marker has a good selection effect on the thioglycoside of the rape seeds.

The invention discloses a method and a device for identifying conservative non-coding elements (CNEs) in a genome. The method comprises the following steps: respectively comparing other genomes in a plurality of high-quality genomes with a reference genome to obtain genome comparison results; identifying the comparison result of the species evolutionary relationship topological structure and the genome to obtain a conserved sequence position information gff file; the gff file is filtered, and a CNEs.gff file is obtained; a CNEs sequence is extracted from the CNEs.gff file; determining a CNEs differentiation sequence according to the CNEs sequence; and performing function enrichment analysis on the target gene of the CNEs differentiation sequence to obtain a plurality of identification results of the CNEs in the high-quality genome. According to the method and the device, the technical problem that identification of the CNEs is incomplete or misjudged due to the fact that identification of the CNEs is often influenced by genome structure variation in the prior art is solved.

The invention relates to a primer of an RBP4 gene molecular marker related to pig weak litter number character and application of the primer, and belongs to the technical field of biology. The RBP4 gene molecular marker is located at the 105043789th base of chromosome 14 of a pig reference genome Sscrofa11.1 version, the base is mutated into A or G, and the genotypes are A / A, A / G and G / G. The number of weak piglets of the G / G genotype pigs is lower than that of the A / G and A / A genotype individuals, and the number of weak piglets of the A / G genotype pigs is lower than that of the A / A genotype individuals. According to the present invention, the forward and reverse primer pair is used to amplify the fragment containing the molecular marker site, and the amplification product is subjected to Sanger sequencing so as to rapidly and accurately identify the genotype of the individual; the molecular marker is used as a genetic marker for pig breeding to breed pigs with less weak piglets; the method can efficiently and accurately detect the weak piglet number character, improves the survival rate of healthy piglets of a pig herd, and has an important value for breeding and production of pigs.

The invention provides a design method of a specific gene probe for detecting pathogenic microorganisms, and belongs to the technical field of microorganism detection. Comprising the following steps: acquiring reference genome data of target pathogenic bacteria, reference genome data of all species belonging to the same genus as the target pathogenic bacteria and genome data of all strains under the target pathogenic bacteria species; comparing the reference genome data of the target pathogenic bacteria with the reference genome data of all target pathogenic bacteria congeneric species by using MUMmer to obtain all fragments in the reference genome of the target pathogenic bacteria, wherein the base number of the fragments is greater than or equal to 20 when the fragments are continuously compared with the reference genomes of other congeneric microorganisms, and the base number of the fragments is greater than or equal to 20 when the fragments are continuously compared with the reference genomes of other congeneric microorganisms; recording starting base sites and ending base sites of all fragments; breaking a reference genome of the target pathogenic bacteria into a 50nt k-mer set, and filtering out non-specific fragments in the 50nt k-mer set to obtain an initial candidate probe set; filtering the initial candidate probe set to obtain a candidate probe set 3; and filtering the candidate probe set 3 to obtain a specific probe set. According to the design method, the specific probe is directly mined in the genomic data of the pathogenic microorganisms, large-scale probe design work can be competent, the designed probe has excellent resolution, the accuracy of pathogenic microorganism detection can be improved, and accurate detection of the pathogenic microorganisms is achieved.

The invention relates to a molecular marker primer related to the feed conversion rate character of Muscovy ducks and application of the molecular marker primer, and belongs to the technical field of molecular detection. According to the molecular marker SNP related to the feed conversion rate character of the muscovy ducks, nucleotide sequences of SNP primers are shown as SEQ ID NO: 1-SEQ ID NO: 2, a first molecular marker is located at the FSTL4 gene GWHBJBF00000015: 17461711 position on the 15 # chromosome in a muscovy duck reference genome KizCaiMos1.0, and a basic group at the position is T or G, and a second molecular marker is located at the position of the FSTL4 gene GWHBJBF00000015: 17461711 position of the 15 # chromosome in the muscovy duck reference genome KizCaiMos1.0. The second molecular marker is located at the FSTL4 gene GWHBJBF00000015: 17461712 position on the No.15 chromosome in the Muscovy duck reference genome KizCaiMos1.0, and the basic group at the position is G or T. The Muscovy duck feed conversion rate screening device has the advantages that Muscovy duck feed conversion rate screening can be achieved, the seed selection accuracy is improved, and the seed selection efficiency is improved.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to sheep fecundity and application of the SNP molecular marker. The SNP molecular marker comprises an SNP1 (Single Nucleotide Polymorphism) and / or an SNP2; the SNP1 is located at a base at the 115747587 site of a chromosome 6 of a sheep reference genome AR-UIRamV2.0 version, and the polymorphism of the SNP1 is T / G; the SNP2 is located at a basic group at the 41419566th site of a chromosome 22 of a sheep reference genome AR-UIRamV2.0 version, and the polymorphism of the SNP2 is A / G. According to the scheme, the SNP molecular marker related to sheep fecundity is provided, sheep with the high fecundity character can be efficiently and accurately identified and screened out, and the marker is high in typing quality, single in copy and high in polymorphism and can be used for sheep fecundity molecular marker assisted breeding.

The invention belongs to the technical field of animal molecular markers, and particularly relates to an SNP molecular marker for beef cattle flavor character breeding and application of the SNP molecular marker. The invention proposes that genotype polymorphism of 84181318bp and 84181699bp on chromosome 3 of an international bovine reference genome AR-UCD1.2 version is related to the character of the content of the beef arachidonic acid for the first time, the genotype polymorphism can be used for evaluating the content of the beef arachidonic acid, and beef cattle with specific genotypes at the two sites has relatively high content of the beef arachidonic acid. The site can be used as a molecular marker for evaluating the content of the arachidonic acid in the beef cattle, early prediction and auxiliary screening of beef cattle individuals with excellent meat quality and flavor characters, and a theoretical basis and a technical support are provided for breeding of the beef cattle with high content of the arachidonic acid in the beef cattle.