773 results about "Reference genome" patented technology

The invention belongs to the field of molecular biological identification, and particularly relates to a specific SNP (Single Nucleotide Polymorphism) site combination for identifying a Wuzhishan pig variety and application. The specific SNP site combination for identifying the Wuzhishan pig variety comprises 77 SNP sites, and the physical positions of the SNP sites are determined by sequence comparison based on a pig reference genome Sscrofa11.1. The specific SNP site combination is screened based on a method of combining whole genome association analysis with selection signal analysis so as to ensure the accuracy of site selection. The selected specific SNP site combination can rapidly realize accurate identification of the Wuzhishan pig variety on the gene level, and has significant application value in the aspects of genetic resource accurate protection and variety utilization of Hainan Wuzhishan pigs.

The invention belongs to the technical field of pepper planting, and discloses a pepper multi-branch gene CaBr1 linked KASP molecular marker as well as a primer, a kit and application thereof, the KASP molecular marker takes pepper S8 (zhangshuang) as a reference genome, and the single nucleotide polymorphism at the 103175415th basic group on the No.8 chromosome is subjected to basic group G-to-A replacement. A chromosome region closely linked with multiple branches of the pepper is obtained by using a BSA (Bovine Serum Albumin) population positioning method, a molecular marker is developed in a candidate region to determine a candidate gene CaBr1, a KASP molecular marker is screened according to base mutation of the candidate gene, and the marker is used for carrying out genotype identification on 90 randomly sampled single plants of an F2 population, and the coincidence rate reaches 100%. The result not only contributes to pepper branch phenotype identification and assistant breeding, but also lays a foundation for map-based cloning of the branch gene and analysis of a molecular mechanism of pepper plant type regulation.

The invention belongs to the technical field of molecular markers, and discloses a molecular marker located in No.18 chromosome and related to the salt tolerance of soybean seeds in the germination period and application of the molecular marker. According to the present invention, the salt tolerance index of the soybean seed germination stage is determined, the stable and reliable molecular marker GmChr1856968562 closely linked with the salt tolerance ability of the seed germination stage is obtained through GWAS correlation analysis, the molecular marker is located at the 56968562 site on the No.18 chromosome of the soybean reference genome Glycine max Wm82. A2. V1, and the marker for KASP detection is developed based on the site, the invention also designs a primer group for amplifying the KASP marker. The invention further provides a method for rapidly identifying the salt tolerance of the soybean seeds in the germination period by using the primer group of the molecular marker, and the method is simple, convenient and rapid, accurate in identification result and good in popularization and application prospect.

The invention relates to the technical field of molecular detection, and particularly discloses a liquid chip for identifying opisthopapus taihangensis and Hebei province as well as a preparation method and application of the liquid chip. Genotyping sites of the liquid chip comprise 1140 SNP sites; the physical positions of the 1140 SNP loci are determined based on comparison of a whole genome sequence of a bovine reference genome, and the version number of the whole genome sequence of the bovine reference genome is ARSUCD 1.2; wherein the physical position information of the 1140 SNP loci is as shown in a table 1. The liquid chip can realize genetic typing of the opisthopapus taihangensis and the Hebei province cattle, has the advantage of high detection rate, and can obviously cluster the opisthopapus taihangensis and the Hebei province cattle into two classes when clustering analysis is carried out on the opisthopapus taihangensis and the Hebei province cattle, so that a marker combination of the liquid chip can represent whole genome re-sequencing genes, assists in distinguishing the opisthopapus taihangensis and the Hebei province cattle, and is high in accuracy and high in accuracy. Therefore, the variety identification of the opisthopapus taihangensis and the Hebei Hebei is realized.

The invention relates to the technical field of bioinformatics data processing, in particular to a genome sequence compression method and system. The method comprises the following steps: acquiring genome sequencing data; comparing the sequencing data with a reference genome to determine a difference site; differentiating the difference sites as sequencing errors or real variations through a time sequence difference neural network model; performing differential compression coding according to an identification result; a friendly variation detection format is constructed, and rapid variation query is supported through a multi-level index structure and a variation metadata table. According to the method provided by the invention, the sequencing error and the real variation can be accurately distinguished through the time sequence differential neural network model, and important biological variation information is protected while the compression efficiency is improved by adopting the differential compression coding strategy.

The invention discloses an indel molecular marker for identifying color and luster of citrus peel in a seedling stage and application of the indel molecular marker. The corresponding sites of the indel molecular marker of the citrus are positioned in chr7: 18, 398, 798-18, 399 and 289 intervals of a chromosome 7 of an SWO3.0 reference genome; the genome DNA of the citrus seedling stage plant is used as a template, the Indel molecular marker is amplified through PCR, the citrus seedling stage plant can be divided into a homozygous insertion type, a homozygous deletion type and a heterozygous type according to a PCR amplification result, and the three types are associated with the color of the citrus fruit for identifying the color of the citrus peel, so that the early screening of the seedling stage target traits of the citrus breeding population is realized, and the early screening of the seedling stage target traits of the citrus breeding population is realized. The breeding period is remarkably shortened, the breeding cost is reduced, and important technical support is provided for citrus fruit color improvement breeding.

The invention belongs to the technical field of molecular biology and genetic breeding, and discloses a molecular marker closely linked with soybean plant height QTL qPH1.01 and application of the molecular marker. According to the present invention, the major QTL qPH1.01 for regulating corn plant height variation is identified by using linkage analysis, the significantly associated SNP is located at the 27th, 318th and 904th bases of the first chromosome of the reference genome Glycinemaxv2.1, 6.28-11.14% phenotypic variation can be explained in the single environment, the PARMS marker developed by using the SNP is used to detect 96 soybean varieties, the typing is clear, the operation is simple and convenient, the method is suitable for soybean plant polymer breeding, and the application prospect is broad. And a new high-yield soybean variety with proper plant height and lodging resistance is cultivated.

The invention provides a beet genetic diversity analysis method based on whole genome re-sequencing. The method comprises the steps of PacBio HiFi library construction, computer sequencing, data information analysis, assembly quality evaluation and Hi-C auxiliary genome assembly. The invention further provides application, and the high-continuity beet reference genome is used for analyzing beet intraspecific genetic diversity and developing high-density molecular markers. According to the method, the high precision of HiFi, the super-long read length of Nanopore and Hi-C space interaction data are combined for the first time, the problems of repeated sequence and complex SV analysis are solved, and all-round breakthrough of genome continuity, annotation integrity, genetic diversity coverage and epigenetic integration is achieved.

The invention belongs to the technical field of molecular biology and genetic breeding, and discloses a molecular marker of a locus qSGC.A8-3 remarkably associated with glucosinolate in brassica napus seeds and application of the molecular marker. A locus qSGC.A8-3 remarkably related to the glucosinolate content of the rape seeds and a peak SNP marker Bn-A08-p12638473 of the locus qSGC.A8-3 are located at 10th, 557th and 348th basic groups of a reference genome A08 chromosome of the rape DarmorV4.1, the average additive effect is 9.6 mu mol / g, and 7.5% of phenotypic variance can be explained. When the PARMS marker designed by the SNP is used for detecting rape germplasm resources, the PARMS marker is simple and convenient to operate and clear in typing, and the average thioglycoside content of AA genotype material seeds is higher than that of GG genotype material seeds, so that the PARMS marker has a good selection effect on the thioglycoside of the rape seeds.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to sheep fecundity and application of the SNP molecular marker. The SNP molecular marker comprises an SNP1 (Single Nucleotide Polymorphism) and / or an SNP2; the SNP1 is located at a base at the 115747587 site of a chromosome 6 of a sheep reference genome AR-UIRamV2.0 version, and the polymorphism of the SNP1 is T / G; the SNP2 is located at a basic group at the 41419566th site of a chromosome 22 of a sheep reference genome AR-UIRamV2.0 version, and the polymorphism of the SNP2 is A / G. According to the scheme, the SNP molecular marker related to sheep fecundity is provided, sheep with the high fecundity character can be efficiently and accurately identified and screened out, and the marker is high in typing quality, single in copy and high in polymorphism and can be used for sheep fecundity molecular marker assisted breeding.

The invention belongs to the technical field of animal molecular markers, and particularly relates to an SNP molecular marker for beef cattle flavor character breeding and application of the SNP molecular marker. The invention proposes that genotype polymorphism of 84181318bp and 84181699bp on chromosome 3 of an international bovine reference genome AR-UCD1.2 version is related to the character of the content of the beef arachidonic acid for the first time, the genotype polymorphism can be used for evaluating the content of the beef arachidonic acid, and beef cattle with specific genotypes at the two sites has relatively high content of the beef arachidonic acid. The site can be used as a molecular marker for evaluating the content of the arachidonic acid in the beef cattle, early prediction and auxiliary screening of beef cattle individuals with excellent meat quality and flavor characters, and a theoretical basis and a technical support are provided for breeding of the beef cattle with high content of the arachidonic acid in the beef cattle.

The invention belongs to the technical field of plant molecular markers, and particularly discloses an Indel molecular marker related to the salt tolerance of brassica napus and application of the Indel molecular marker. The marker is located at the position of an A01 chromosome scafoldA0133077947 of a brassica napus reference genome ZS11.v10, and the genotype of a polymorphic site is CTAA or C. The invention further discloses a method for identifying the marker of the brassica napus. Large-scale group phenotypic association analysis proves that the molecular marker is remarkably associated with the salt tolerance of the brassica napus in the germination period, and when the polymorphic site genotype is CTAA, the salt tolerance in the germination period is high; when the genotype of the polymorphic site is C, low salt tolerance in the germination period is shown. The invention finds that the polymorphic site is obviously associated with the salt tolerance of the brassica napus for the first time, provides a powerful molecular tool for solving the problems of difficulty in phenotype identification, long period and low efficiency in salt tolerance breeding of the brassica napus, can accurately screen salt-tolerant materials in the early stage, and greatly accelerates the breeding process of new varieties of the salt-tolerant brassica napus.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to a Hu sheep body height character and application thereof, and belongs to the field of gene engineering. The SNP molecular marker is located at the nucleotide at the 71034104th site of the No.17 chromosome of the sheep reference genome AR-UIRambv3.0 version, the polymorphism of the site is T or C, and the genotype is TT, TC or CC. Correlation analysis verifies that the body height of a TT genotype Hu sheep individual is obviously higher than that of TC genotype and CC genotype individuals. The invention further provides a primer for detecting the SNP molecular marker and application of the primer in Hu sheep body height character auxiliary screening. By utilizing the molecular marker, dominant genotype individuals can be quickly and accurately identified in the young Hu sheep period without waiting for the Hu sheep to grow to the body size for determining the age, the breeding efficiency of the Hu sheep body height can be greatly improved, and the molecular marker has important significance for breeding large Hu sheep strains and improving the breeding economic benefits.

The invention is suitable for the technical field of soybean breeding, and provides a KASP molecular marker for soybean plant height identification and application of the KASP molecular marker. The KASP molecular marker is based on an SNP variation site of a Glyma. 19G206100 gene between two parents of Chinese flat stem soybean and F02, the mutation position of the Glyma. 19G206100 gene is the 312th basic group on a CDS sequence, C of a reference genome is mutated into G, the 104th amino acid on a protein sequence is mutated into lysine from asparagine, and the 104th amino acid on the protein sequence is mutated into lysine from asparagine. The primer of the KASP molecular marker comprises a forward primer with a nucleotide sequence as shown in SEQ ID NO.1-2 and a reverse primer with a nucleotide sequence as shown in SEQ ID NO.3. The invention further discloses a kit for detecting the KASP molecular marker. The KASP molecular marker is developed on the basis of the polymorphic site of Gm19G206100 in germplasm resources, the marker has the advantages of being high in resolution ratio and easy to detect, can be applied to plant height selection of early generations of soybean cross breeding, has the effects of reducing the breeding scale, reducing the workload and improving the breeding efficiency, and has important application value in production.

The invention belongs to the technical field of biology, and discloses a megalobrama amblycephala hypoxia-resistant snp molecular marker and detection and application thereof, the number of SNP sites of the SNP molecular marker related to megalobrama amblycephala hypoxia-resistant characters is three, the SNP sites are located at the 14321966 nucleotide site, the 14323457 nucleotide site and the 14323789 nucleotide site of a megalobrama amblycephala reference genome CM032046.1 respectively, the polymorphisms are G / A, T / C and C / A respectively, and the SNP sites are named as SNP1, SNP2 and SNP3 respectively. Based on the SNP molecular marker, the hypoxia-resistant character of megalobrama amblycephala can be identified by detecting the genotype of the SNP site, and the hypoxia-resistant megalobrama amblycephala can be screened, so that the breeding of the hypoxia-resistant variety of megalobrama amblycephala is accelerated, and the stable development of the megalobrama amblycephala culture industry is promoted.

The invention relates to the technical field of genomics, molecular biology, bioinformatics and whole-genome selective breeding, in particular to a whole-genome liquid chip for apostichopus japonicus and application of the whole-genome liquid chip. The liquid phase chip contains sequences of background SNP loci and functional SNP loci which are used for gene analysis and are positioned on an apostichopus japonicus reference genome; wherein the functional SNP sites are associated with important economic characters of the apostichopus japonicus. The invention also discloses application of the liquid chip in apostichopus japonicus genome selective breeding, important economic character gene positioning, genetic diversity analysis, germplasm resource improvement and protection.

The invention discloses a structural variation molecular marker located on a pig chromosome 7 and related to the lactation ability of a sow. The structural variation molecular marker is a DNA (deoxyribonucleic acid) fragment which is inserted between the 112024896 bp site and the 112024896 bp site on a chromosome 7 of an international pig reference genome 11.1 version, wherein the nucleotide sequence of the DNA fragment is shown as SEQ ID NO: 1; the genotype of the gene is T / T, T / INS and INS / INS. The structural variation molecular marker provided by the invention obviously influences the total weight gain character and the lactation ability character of the 7-day-old sow, and by breeding the pig with the structural variation molecular marker genotype of T / T, the lactation ability of the sow can be obviously improved, and the survival rate and the weaning weight of piglets are increased.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker positioned on a No.15 chromosome of a sow and related to the lactation ability of the sow. The SNP site of the SNP molecular marker corresponds to the Agt at the 31884611bp position on the No.15 chromosome of an international pig reference genome version 11.1; g mutation; the genotype of the gene is AA, AG or GG. The SNP molecular marker provided by the invention is remarkably related to the sow lactation ability character, the identification of the sow lactation ability character can be realized by identifying the single nucleotide polymorphism of the SNP molecular marker and / or the genotype of the SNP molecular marker, and the dominant allele frequency can be increased generation by generation and the sow lactation ability can be improved by preferably selecting the dominant allele G of the SNP provided by the invention. The pig genetic improvement progress is accelerated, so that the economic benefit of breeding pig breeding is effectively improved.

The invention provides a microbial ecological interaction mechanism determination method and device, and relates to the technical field of species correlation research. The method comprises the following steps: determining N microorganisms and abundance thereof based on metagenome data of a target environment sample; determining the abundance grade and correlation based on the abundance of the ith microorganism and the jth microorganism; carrying out metabolic function annotation on the i microorganism and the j microorganism based on a reference genome, and calculating a function overlapping ratio; determining a community construction mechanism based on the abundance correlation and the function overlapping ratio; based on the function overlapping difference, determining a function overlapping grade change; and determining the ecological interaction mechanism of the i and j microorganisms according to the information. The method can be used for systematically revealing a species coexistence mechanism and a metabolic interaction relationship thereof in the microbial community.

The application relates to the technical field of gene chips, in particular to a SNP molecular marker combination of Pinus massoniana and application thereof. The SNP molecular marker combination is composed of 113,709 SNP molecular markers, wherein the physical position of the SNP molecular markers is determined by sequence alignment based on a reference genome Pinus.tabuliformis V1.0 of Pinus tabulaeformis. The application provides a whole genome chip of Pinus massoniana containing 113,709 SNP sites for the first time, and the chip has the advantages of high efficiency, low cost, good genetic stability and the like, and can be widely applied to different application scenarios such as identification of Pinus massoniana germplasm resources, genetic background analysis of breeding materials, gene positioning, whole genome association analysis, whole genome selection breeding and intelligent design breeding.

The invention belongs to the technical field of soybean molecular genetic breeding, and discloses a KASP marker for detecting an SNP site related to a soybean petiole included angle and application of the KASP marker. The SNP site is located at the 4589285th site on a chromosome 10 of a soybean reference genome Glycine max Wm82. A4. V1, and the site is subjected to C / T basic group replacement. Three KASP primers as shown in SEQ ID NO.2-4 are developed according to the SNP site, PCR amplification and genetic typing are performed on to-be-detected soybeans by using the primer group, and if a detection result shows that the genetic typing of the marker position is 1 / 1, it is judged that the petiole angle of the soybean variety is large; and if the detection result is 0 / 0, judging that the petiole angle is small. The SNP molecular marker can be used for molecular marker-assisted selection of soybean petiole included angle characters, and has important theoretical and practical guiding significance for accelerating genetic improvement of soybean compact plant types and improving breeding selection efficiency. The marker can rapidly identify the soybean petiole included angle, the method is simple and rapid, the identification result is accurate, and the marker has good popularization and application prospects.

The invention discloses a GmGID1-2 gene molecular marker obviously associated with soybean plant height, yield, grease content and protein content and application of the GmGID1-2 gene molecular marker. The molecular marker contains an SNP (Single Nucleotide Polymorphism) site obviously associated with soybean plant height, yield, grease content and protein content, the site is located in a GmGID1-2 gene promoter region, Wm82. A2. V1 is taken as a reference genome, the site is located at 36395036bp of a soybean No.3 chromosome, allelic variation is carried out to obtain a basic group C or T, and the nucleotide sequence is shown in SEQ ID NO: 1. Compared with the soybean with the genotype T, the soybean with the genotype C has the advantages of lower plant height, lower seed protein content, higher yield and higher seed oil content. The dCAPS molecular marker developed according to the SNP can be used for molecular marker-assisted selection, the soybean breeding efficiency is improved, and the soybean breeding process is accelerated.

The invention relates to the technical field of molecular markers, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker related to immune traits of male semi-fine wool sheep and application of the SNP molecular marker. The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to immune traits of elephant male semi-fine wool sheep. The SNP molecular marker is positioned at the 24648039th basic group on the fifth chromosome of a Lanblet sheep reference genome ARS-UIRambv3.0, GCF016772045.2, and the SNP molecular marker is positioned at the 24648039th basic group on the fifth chromosome of the Lanblet sheep reference genome ARS-UIRambv3.0; the mutant basic group is G or T. By detecting the basic group of the 24648039th nucleotide site on the fifth chromosome of the elephant male semi-fine wool sheep, the content of immune globulins IgA, IgG and IgM of an elephant male semi-fine wool sheep individual can be judged, and a new SNP molecular marker resource is provided for non-diagnostic elephant male semi-fine wool sheep immune character marker-assisted selection.

The invention provides a goat whole genome SNP locus combination which is composed of 30000 SNP loci, the physical positions of the SNP loci are determined through sequence alignment based on a goat reference genome ARS1, and locus information is shown in a table 1 in the specification. The invention also provides a liquid phase chip relating to the SNP site combination and an application thereof. The liquid chip has the following advantages: the liquid chip supports flexible screening and updating of original sites, is low in detection cost, is more suitable for genotype screening and typing work of large-scale farms, and helps to improve the breeding efficiency; the liquid-phase chip is excellent in performance, high in average detection rate of SNP sites, stable in typing effect and high in gene consistency; the method can be accurately applied to multi-character breeding practice; according to the method, the integrity of whole genome detection is remarkably improved in design, the covered functional sites are more comprehensive, the marking density and the resolution ratio are higher, the universality is enhanced, and the method is suitable for germplasm resource improvement and protection work of various goats.

The invention belongs to the technical field of molecular biology, bioinformatics, sorghum genetics and whole-genome gene chips, and particularly relates to a sorghum whole-genome SNP molecular marker combination developed based on a targeted sequencing technology, a 10K liquid chip and application of the sorghum whole-genome SNP molecular marker combination and the 10K liquid chip. The SNP molecular marker combination comprises 10,000 SNP loci, and the specific information of the 10,000 SNP loci is as shown in a table 1; the physical positions of the 10000 SNP loci are determined by performing sequence alignment based on a reference genome of the wine red tassel sorghum variety HYZ-T2T with the version number of v1.0. A liquid chip developed based on the SNP molecular marker combination provided by the invention can be effectively applied to genetic diversity analysis, whole genome association analysis, genetic typing, molecular marker-assisted selective breeding, whole genome selective breeding and other applications of sorghum germplasm resources.

The invention belongs to the technical field of crop molecular markers, and particularly discloses a molecular marker for identifying the salt tolerance of brassica napus in the germination period and application of the molecular marker. The molecular marker is located at the position of an A02 chromosome scafoldA028707654 of a brassica napus reference genome ZS11.v10, and the allelotype of the molecular marker is wild type C or mutant type CATATT insertion. The invention also provides a specific primer group for detecting the molecular marker, and the salt tolerance (sensitive type or tolerance type) of the brassica napus line can be quickly identified according to the existence of 411bp and 416bp bands through double PCR amplification and electrophoresis detection. The molecular marker is significantly associated with salt tolerance phenotypes, the detection method is rapid, accurate and low in cost, the molecular marker can be used for brassica napus salt tolerance germplasm resource screening and molecular marker-assisted breeding, and the breeding efficiency is significantly improved.

The application belongs to the technical field of molecular breeding of Brassica napus, and particularly relates to a SNP molecular marker related to the thousand seed weight of Brassica napus and application thereof. The SNP molecular marker is located at the position of 43,480,336 of the scaffold C05 chromosome of Brassica napus reference genome ZS11 v0. The site has T / G polymorphism, wherein the T allele is a favorable allele variation for increasing the thousand seed weight of Brassica napus. The SNP molecular marker can be used for screening high thousand seed weight potential materials at the seedling stage or early stage through DNA detection, improving the high yield breeding efficiency of Brassica napus, and can be used for screening high thousand seed weight materials of Brassica napus, molecular assisted selection breeding, evaluation of germplasm resources and improvement of yield-related traits.

This invention relates to soybean pod-resistant trait loci. qPD08‑1 This invention relates to the soybean pod-bomb resistance gene locus and its applications, belonging to the field of plant molecular biotechnology. qPD08‑1 Located in soybeans Glyma The reference genome of .Wm82.a2.v1, located between 35983663 and 36106921 bp on chromosome 8, has a total physical interval length of 123.258 kb. The molecular marker detection method of this invention can be used for identification of soybean pod-resistant germplasm resources, screening and functional studies of pod-resistant genes, marker-assisted selection breeding for pod-resistant soybeans, and natural resource applications. It features convenient detection, stable amplification products, and high specificity, helping to broaden the sources of superior breeding parents and improve the efficiency of soybean breeding selection.

This invention relates to the fields of genomics, molecular biology, bioinformatics, and genome-wide selection breeding, specifically a 20k liquid-phase breeding chip for the whole genome of *S. esculenta* and its applications. The liquid-phase chip contains background SNPs and functional SNPs located on the *S. esculenta* reference genome; wherein the background SNPs are uniformly distributed within the genome; and the functional SNPs are associated with important economic traits of *S. esculenta*; these important economic traits include one or more of the following: saponin content, polysaccharide content, and heat tolerance. The chip can be applied to the assessment of genetic diversity in *S. esculenta*, identification of germplasm resources and phylogenetic relationships, genome-wide association analysis of important economic traits, and genome-wide selection breeding. This chip has advantages such as high throughput, high region coverage, high locus detection rate, and high flexibility, providing powerful tool support for molecular breeding of *S. esculenta*.

The invention relates to the technical field of animal genetic breeding and genomics, and discloses a reference genome of a chromosome level of a Zhaotong Wujin pig and application of the reference genome. The high-quality chromosome level reference genome of the Zhaotong Wujin pig is constructed for the first time. The total length of the genome is about 2.66 Gb, the chromosome anchoring rate is more than 98%, Contig N50 is about 102Mb, and the integrity evaluation of the genome is more than 98%; 21507 protein coding genes are annotated in total. Compared with the existing reference sequence, the genome disclosed by the invention contains specific sequence variation and gene family information of local pig breeds, and provides a more accurate and comprehensive genetic blueprint. The genome resource can be widely applied to molecular mechanism research related to traits such as meat quality, reproductive capacity, growth speed, disease resistance and the like, molecular marker development, breeding chip design, quantitative trait locus (QTL) positioning, genetic evaluation, gene editing, transgenic breeding, germplasm resource protection and the like. The method has important application value in genetic improvement of Chinese local pig breeds.