1502 results about "Reference genome" patented technology

The invention relates to the technical field of molecular markers, and discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the litter size character of pigs and application of the SNP molecular marker. The SNP molecular marker is located at a 34977540bp site of a chromosome 2 of an international pig reference genome Sscrofa11.1 version, and the nucleotide sequence of the site is G or A and is located in an exon region of an LUZP2 gene. The invention also discloses an application of the SNP molecular marker in breeding of the litter size character of the boar, and provides a method for breeding or auxiliary breeding of the boar with high / low litter size based on the marker. The invention also provides a specific primer for identifying the SNP site. By applying the technologies, accurate prediction and efficient screening of the litter size of the large white pigs can be achieved, the breeding efficiency of excellent breeding pigs is remarkably improved, and the population improvement process is accelerated. The invention also provides important technical support for sustainable development of pig raising industry, also provides theoretical basis for research in related fields, and is expected to promote further development of pig genetic improvement work.

The invention relates to the technical field of molecular biology and molecular markers, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker located on a porcine chromosome 14 and related to porcine reproductive traits and application. The SNP site of the SNP molecular marker located on the No.14 chromosome of the pig and related to the reproductive traits of the pig corresponds to the Cgt at the 105043272th site on the No.14 chromosome of the international pig reference genome version 11.1; t mutation is carried out, and the polymorphism of the basic group at the site affects the porcine reproductive traits; wherein the reproductive performance of pigs with CC and TC genotypes is superior to that of pigs with TT genotypes. By optimizing the dominant alleles of the SNP molecular marker, the frequency of the dominant alleles can be increased generation by generation, the reproductive performance of pigs is improved, and the genetic improvement progress of the pigs is accelerated, so that the economic benefit of pig breeding is effectively improved.

The invention belongs to the technical field of molecular biotechnology and molecular markers, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker located on a chromosome 2 and related to abnormal delivery traits of sows and application of the SNP molecular marker. An SNP site of the SNP molecular marker corresponds to Cgt at the 23330829bp position on a chromosome 2 of an international pig reference genome version 11.1; and the polymorphism of the basic group at the site affects the abnormal delivery character of the sow. The method solves the problem of genetic improvement of abnormal delivery of sows by means of molecular breeding, and can increase the frequency of dominant alleles generation by generation, reduce the occurrence rate of abnormal delivery of sows and improve the reproductive performance of sows by optimizing the dominant alleles of variation sites. The pig genetic improvement progress is accelerated, so that the economic benefit of breeding pig breeding is effectively improved.

The invention relates to an STON1 gene molecular marker primer related to chicken double-yolk egg traits and application of the STON1 gene molecular marker primer, and belongs to the technical field of biology. The STON1 gene molecular marker is a 7867409th basic group of a chicken reference genome GRCg7b version chromosome 3, the basic group is mutated into A or G, and the genotypes are A / A, A / G and G / G. The double-yolk egg ratio of the G / G genotype chicken is higher than that of the A / G and A / A genotype individuals, and the double-yolk egg ratio of the A / G genotype chicken is higher than that of the A / A genotype individuals. A forward and reverse primer pair is used for amplifying a fragment containing the molecular marker site, Sanger sequencing is carried out on an amplification product, the genotype of an individual is rapidly and accurately identified to serve as a genetic marker for chicken breeding, and the chicken with the higher proportion of double-yolk eggs is bred; the double-yolk egg traits can be efficiently and accurately detected, the breeding accuracy and breeding efficiency of the chicken double-yolk egg proportion are improved, and the method has important value for chicken breeding and production.

The invention discloses an SNP molecular marker related to the conception rate of young cows, a detection product and application of the SNP molecular marker, and belongs to the technical field of dairy cow breeding markers. The SNP molecular marker is chr6: g. 103355581Cgt, and the SNP molecular marker is a molecular marker. The method comprises the following steps of: taking a bovine reference genome version UMD3.1 as a standard for a coordinate position, numbering Assessment in NCBI (National Center of Biotechnology Information) as GCF000003055.6, and numbering a chromosome as AC 000163.1; the young cows are cows which are 12-24 months old and do not lay calves. By detecting the genotype of the SNP site, the conception rate condition can be effectively judged, parent individuals with the higher young cow conception rate can be screened, early selection of genotype individuals with the high young cow conception rate is achieved, the frequency of the genotype with the high young cow conception rate in a dairy cow group is increased, and the reproduction rate of the dairy cow group is increased; the service life of cows is prolonged, the treatment cost is saved, the breeding cost is reduced, and the pasture income is increased.

The invention belongs to the field of molecular biological identification, and particularly relates to a specific SNP (Single Nucleotide Polymorphism) site combination for identifying a Wuzhishan pig variety and application. The specific SNP site combination for identifying the Wuzhishan pig variety comprises 77 SNP sites, and the physical positions of the SNP sites are determined by sequence comparison based on a pig reference genome Sscrofa11.1. The specific SNP site combination is screened based on a method of combining whole genome association analysis with selection signal analysis so as to ensure the accuracy of site selection. The selected specific SNP site combination can rapidly realize accurate identification of the Wuzhishan pig variety on the gene level, and has significant application value in the aspects of genetic resource accurate protection and variety utilization of Hainan Wuzhishan pigs.

The invention discloses a KASP molecular marker for identifying soybean hundred-grain weight and application thereof, and relates to the technical field of biology and application of an SNP locus, the SNP locus takes a soybean Wm82. A2. V1 genome sequence as a reference genome, the SNP is the 16321243th SNP on a soybean 11 # chromosome, and the SNP is the 16321243th SNP on the soybean 11 # chromosome. Corresponding to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, when the locus is CC homozygosis, the corresponding genotype is A; when the locus is TT homozygous, the corresponding genotype is B; according to the application, soybeans with different hundred-grain weights are screened or screened in an auxiliary mode, and the different hundred-grain weights of the soybeans are as follows: the homozygous soybeans with the genotype A are higher than or candidate to be higher than the homozygous soybeans with the genotype B. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding progeny materials with high hundred-grain weight.

The invention provides an SNP molecular marker combination for pop corn whole genome typing, a detection chip and application, and relates to the technical field of molecular breeding. The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker combination for genotyping of the whole genome of the pop corn, a genotyping object comprises 6419 SNP loci, the SNP markers uniformly cover the whole corn reference genome, and a high-quality molecular marker can be provided for map-based cloning of a pop corn specific agronomic trait control gene. Meanwhile, different types of corn can also be subjected to genotyping. Burst corn germplasm resources are narrower than other types of corn germplasm resources, and the chip can overcome the defect that an existing chip developed based on grain corn diversity is low in diversity in pop corn genotyping.

The invention belongs to the technical field of molecular biomedicine, and provides a colorectal cancer early screening method based on multi-omics sequencing, which comprises the following steps: collecting a plasma sample to be detected, collecting sequencing data to be detected, and classifying a colorectal tumor early screening model. The training process of the colorectal tumor early screening model comprises the steps of original sequencing data acquisition, quality control and human reference genome comparison, reference methylation data acquisition, tumor specific region screening, plasma cfDNA sequencing data acquisition, coordinate determination sequencing data acquisition, methylation feature set screening and terminal motif feature screening. And carrying out breakpoint motif feature screening, feature merging and model training. By constructing the colorectal tumor early screening model, the non-invasive colorectal cancer detection method with high cost effectiveness is realized, the economic cost of sequencing is reduced, and the accuracy and sensitivity of detection are remarkably improved.

The invention relates to the technical field of crop genetic breeding and plant molecular design breeding, in particular to a pakchoi whole genome liquid phase chip and application. The pakchoi breeding gene chip disclosed by the invention comprises a target capture probe which is designed by taking 1000 core SNP (Single Nucleotide Polymorphism) molecular markers positioned on 40KSNP sites on a pakchoi reference genome version V4.0 as templates. The pakchoi whole genome liquid phase chip provided by the invention comprises a 40K site targeted capture probe mixed solution and a hybridization capture reagent. The pakchoi 40KSNP targeted capture probe can rapidly and effectively track genetic materials of pakchoi, and is suitable for different application scenes such as accurate identification of pakchoi germplasm resources, genetic relationship analysis between materials, variety authenticity identification, QTL gene positioning and mining, molecular marker-assisted breeding, variety improvement and whole genome selective breeding application. Therefore, the method has good application prospect and important social value.

The invention discloses an SNP molecular marker related to the backfat thickness character of a pig living body with the weight of 100 kg and application of the SNP molecular marker. SNP typing data is obtained by integrating re-sequencing data of the pig and comparing the re-sequencing data of the pig to a pig reference genome Sscrofa11.1, whole genome analysis is conducted through a mixed linear model, 5053 SNP sites related to the backfat thickness of the pig living body with the weight of 100 kg are screened out, and the SNP molecular marker related to the backfat thickness character of the pig living body with the weight of 100 kg is obtained. The correlation between the SNP sites and the backfat thickness character of the pig living body with the weight of 100 kg reaches a remarkable level, a new molecular marker is provided for auxiliary selection and improvement of the backfat thickness character of the pig living body with the weight of 100 kg, and the molecular marker has important significance for auxiliary breeding of pigs.

The invention discloses an SNP molecular marker related to drought resistance of corn and application of the SNP molecular marker, and relates to the field of agricultural molecular biology. The SNP molecular marker comprises at least one of a first SNP molecular marker and a second SNP molecular marker; the first SNP molecular marker is located at a basic group at a 97605381 site of a chromosome 9 of a corn reference genome Zeamas.AGPv4, and the polymorphism of the first SNP molecular marker is C / T; the second SNP molecular marker is located at the basic group of the 97604011 site of the No.9 chromosome of the corn reference genome Zeamas.AGPv4, and the polymorphism is C / T. The SNP molecular marker disclosed by the invention has the characteristics of high specificity, high sensitivity, high resolution and high typing quality; the drought resistance of the corn material can be quickly judged according to the genotype, and the method has wide application universality and can be used for corn breeding.

The invention relates to the technical field of molecular biology, and particularly provides an InDel molecular marker related to the size of a lychee kernel and application of the InDel molecular marker. The InDel molecular marker is located at a site 25610680 of a fifth chromosome of a reference genome GeneBank: GCA019925255.1 of the litchi, and the InDel molecular marker is used for detecting the DNA of the litchi. The genotype of the marker in a pyrokaryotic litchi material is homozygous' GAAAAA / GAAAAA ', and the genotype of the marker in a macrokaryotic litchi material is heterozygous' G---- / GAAAAA 'or homozygous' G---- / G----'. The invention further provides a primer group for detecting the molecular marker, an effective tool is provided for molecular marker-assisted breeding of the size of the lychee kernel, and the method is expected to assist in shortening the breeding period, improving the breeding efficiency and promoting the improvement work of the lychee variety.

The invention discloses a whole-genome low-density chip for breeding high-yield and long-life characters of dairy cows and application of the whole-genome low-density chip. The invention firstly discloses a whole-genome low-density chip for breeding the high-yield and long-life character of the dairy cow, the whole-genome low-density chip comprises 10000 SNP (Single Nucleotide Polymorphism) loci, ARS-UCD1.2 is used as a reference genome, and the information of the 10000 SNP loci is shown in a table 6. The invention further discloses application of the whole-genome low-density chip. The 10,000 SNP loci contained in the whole genome low-density chip are uniformly distributed on chromosomes, cover the whole genome and important character loci, can effectively improve the longevity and the accuracy of milk yield breeding, and can be applied to gene screening, gene positioning, marker-assisted breeding and the like. In addition, the detection cost of the low-density chip is greatly reduced, and the low-density chip is more suitable for commercialized dairy farms and has important significance on dairy cow breeding and dairy cow group improvement.

The invention discloses a tetraploid crassostrea gigas parent-child relationship identification method based on SNP sites, and belongs to the technical field of tetraploid crassostrea gigas molecular assisted breeding. The method for identifying the parent-child relationship of tetraploid crassostrea gigas comprises the following steps: (1) sequencing adductor muscles of tetraploid crassostrea gigas to be detected; (2) comparing the reference genome of the crassostrea gigas to obtain the genotype of the specified SNP site of the tetraploid crassostrea gigas; (3) calculating IBS and LOD of the to-be-detected parents and the to-be-detected offspring according to an SNP typing result; and (4) identifying the parent-child relationship between the to-be-detected parent and the filial generation by combining IBS and LOD. The SNP loci have the advantages that 1000 SNP loci for identifying the parent-child relationship of the tetraploid crassostrea gigas are disclosed for the first time, data support is provided for identifying the parent-child relationship of the tetraploid crassostrea gigas, a foundation is laid for developing a liquid phase chip for identifying the parent-child relationship of the tetraploid crassostrea gigas, and the SNP loci have good application prospects.

The invention discloses an SNP (Single Nucleotide Polymorphism) site and a KASP primer group for identifying or assisting in identifying clematis maple and application of the SNP site and the KASP primer group. The SNP site is located at the 2688th site of SEQ ID No.1 (sequence identifier number 1). The KASP primer group is composed of single-stranded DNA (deoxyribonucleic acid) as shown in SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 4. The method comprises the following steps: carrying out next-generation sequencing and assembling on clematis maple for the first time, taking formed ultra-long scaffold as a reference genome ultra-long fragment, carrying out simplified genome sequencing on 15 clematis plants, and comparing the simplified genome sequencing with the reference genome ultra-long fragment to obtain difference sites, finally, the KASP molecular marker CA24547 is developed on the basis of the difference sites, and the KASP molecular marker CA24547 is verified. The KASP molecular marker developed by the invention can realize accurate identification of the clematis nepalensis in the seedling stage, and has important significance for protecting the clematis nepalensis.

The invention belongs to the technical field of molecular biotechnology and molecular markers, and particularly relates to an SNP (Single Nucleotide Polymorphism) site related to pig intramuscular fat content, a related molecular marker and application of the SNP site. The SNP site corresponds to the 151330919bp site of a No.6 chromosome of an international pig reference genome version 11.1, the polymorphism of a base of the site affects the intramuscular fat content of the pig, the related index of a TT genotype is greater than that of a pig individual with TG and GG genotypes, and the related index of the TG genotype is greater than that of a pig individual with GG genotype. According to the invention, a molecular breeding means is utilized to solve the problem of improving the character expression of the intramuscular fat content of the pig, and by optimizing the dominant alleles of the SNP, the frequency of the dominant alleles can be increased generation by generation, the intramuscular fat content of the pig can be improved, the pork quality can be improved, the genetic modification breeding of the pig can be accelerated, and the economic benefit of a pig farm can be improved.

Systems and methods for outbreak analysis by performing taxonomic classification of the first LRS data to assign one or more taxonomic identifiers to each record in the first LRS data; identifying a plurality of species-specific candidate genomes from a reference genome database based on the assigned taxonomic identifiers; aligning the first LRS data with each of the plurality of species-specific candidate genomes to determine a first species identity of a microorganism species present in the sample; identifying a first nearest strain of the identified microorganism species present in the first sample by aligning the first LRS data with a plurality of strain-specific candidate genome dataset.

The invention belongs to the technical field of pepper planting, and discloses a pepper multi-branch gene CaBr1 linked KASP molecular marker as well as a primer, a kit and application thereof, the KASP molecular marker takes pepper S8 (zhangshuang) as a reference genome, and the single nucleotide polymorphism at the 103175415th basic group on the No.8 chromosome is subjected to basic group G-to-A replacement. A chromosome region closely linked with multiple branches of the pepper is obtained by using a BSA (Bovine Serum Albumin) population positioning method, a molecular marker is developed in a candidate region to determine a candidate gene CaBr1, a KASP molecular marker is screened according to base mutation of the candidate gene, and the marker is used for carrying out genotype identification on 90 randomly sampled single plants of an F2 population, and the coincidence rate reaches 100%. The result not only contributes to pepper branch phenotype identification and assistant breeding, but also lays a foundation for map-based cloning of the branch gene and analysis of a molecular mechanism of pepper plant type regulation.

The invention relates to a molecular marker closely linked with a pepper fruit length regulation gene CaFL1 and application of the molecular marker. The molecular marker is insertion / deletion of three basic groups GAC at 223149123-223149125th positions of chromosome 10 of a pepper genome, and the position of the molecular marker in the genome is determined according to a Qieme reference genome. Specifically, when the genotype of the molecular marker is InGAC: InGAC, the pepper has a long fruit phenotype, when the genotype is InGAC: DelGAC, the pepper has an intermediate phenotype, and when the genotype is DelGAC: DelGAC, the pepper has a short fruit phenotype. Through verification, the accuracy rate of the molecular marker reaches 100%, early, massive and accurate prediction and directional improvement of the pepper fruit length can be realized, and a new way is provided for screening and identifying the pepper fruit length and breeding the pepper fruit length.

The invention discloses a biological complex trait key gene identification method, device and equipment and a medium, and the method comprises the following steps: obtaining biological reference genome data, and carrying out orthologous gene class group division on a protein sequence coded by the biological reference genome data; determining a characteristic value data set according to the number of the subgroup sequence and the gene number of the subgroup sequence obtained after division; obtaining each piece of biological phenotype data; utilizing a machine algorithm model to construct a phenotype matrix according to the biological phenotype data and the characteristic value data set so as to train a phenotype prediction model, and determining the influence weight of each subgroup sequence on the biological phenotype according to the phenotype prediction model; and according to each influence weight, determining a key gene for regulating and controlling the biological complexity. The method provided by the invention can realize more accurate, efficient and comprehensive identification of key genes of prokaryotes and high biological complexity characters.

The invention discloses a method for identifying fish genome structure variation based on a graph genome and application, and the method comprises the following steps: 1) respectively comparing fish individual genome data of different germplasm with a reference genome to determine variation information between each individual genome sequence and the reference genome; 2) constructing a graphical generic genome by using variation information; and 3) comparing the genome data of the fish individuals to be identified with the graphical generic genome to generate a genome structure variation data set of the fish individuals to be identified. The method can comprehensively and accurately identify fish genome structure variation, can identify specific structure variation, provides a scientific basis for fish genetic improvement, breeding and resource protection, and is suitable for popularization and application.

The invention relates to the technical field of crop molecular genetic markers, and discloses an InDel molecular marker of avena nuda and application thereof.The molecular marker is a group of 314bp deletion variation and 2bp insertion variation in the 610439484-610440026 interval of the 5C chromosome position of a reference genome of an avena nuda variety OT3098, the nucleotide sequence of the deletion variation is shown as SEQ ID NO.1, the nucleotide sequence of the insertion variation is shown as SEQ ID NO.2, and the nucleotide sequence of the insertion variation is shown as SEQ ID NO.3. The nucleotide sequence of the insertion variation is 5 '-AA-3'. The molecular marker provided by the invention can realize stable detection in agarose gel electrophoresis by combining a specific primer pair (SEQ ID NO.2-3) with an optimized touchdown PCR (Polymerase Chain Reaction) program, has the advantage of being not influenced by a tissue part and a growth period, and provides an efficient and reliable technical means for oat germplasm identification, seed purity detection and molecular breeding.

The invention discloses a KASP molecular marker for identifying soybean protein content and application, and relates to the technical field of biology and application of an SNP locus, the SNP locus takes a soybean Wm82.a2.v1 genome sequence as a reference genome, is the 42148495th SNP on a soybean chromosome 9 and corresponds to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, and when the locus is TT homozygosis, the corresponding genotype is A; when the site is AA homozygous, the corresponding genotype is B; according to the application, different protein contents of soybeans are screened or screened in an auxiliary mode, and the different protein contents of the soybeans are as follows: the homozygous soybeans of the genotype A are higher than or candidate to be higher than the homozygous soybeans of the genotype B. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding offspring materials with high protein content.

The invention discloses a KASP molecular marker for identifying the soybean oil content and application, and relates to the technical field of biology and application of an SNP locus, the SNP locus takes a soybean Wm82. A2. V1 genome sequence as a reference genome, the SNP is the 6562032th SNP on a soybean 20 # chromosome, and the SNP is the 6562032th SNP on the soybean 20 # chromosome. Corresponding to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, when the site is GG homozygosis, the corresponding genotype is A; when the site is AA homozygous, the corresponding genotype is B; according to the application, soybeans with different oil contents are screened or screened in an auxiliary mode, and the different oil contents of the soybeans are as follows: the soybean with the homozygous genotype A is higher than the soybean with the homozygous genotype B or the soybean with the homozygous genotype B in a candidate mode. The invention has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding progeny materials with high oil content.

The invention relates to a CCDC171 gene molecular marker primer related to egg weight character and application of the CCDC171 gene molecular marker primer, and belongs to the technical field of biology. The CCDC171 gene molecular marker is located at the 32551904 base of a Z chromosome of a chicken reference genome GRCg7b version, the base is mutated into C or T, and the genotypes are C / C and T / T. And the egg weight of the C / C genotype chicken is higher than that of the T / T genotype individual. According to the present invention, the forward and reverse primer pair is used to amplify the fragment containing the molecular marker site, and the amplification product is subjected to Sanger sequencing so as to rapidly and accurately identify the genotype of the individual; the molecular marker can be used as a genetic marker for chicken breeding to breed chicken with proper egg weight; the egg weight character can be efficiently and accurately detected, the uniformity of the egg weight of chicken flocks is improved, and the method has important value for chicken breeding and production.

The invention relates to an SNP molecular marker related to duck cervical vertebra length and application thereof, and belongs to the technical field of molecular biology. According to the research, the SNP locus related to the cervical vertebra length character of the meat duck exists on the NC040050.1 chromosome of the reference genome PBH1.5 version of the melanmen duck for the first time by utilizing whole genome association analysis, the locus obviously influences the cervical vertebra length of the 10-week-old melanmen duck, and the CC genotype shows an excellent genotype. Therefore, the invention provides a pair of upstream and downstream primers for amplifying the flanking sequence of the molecular marker, performing genetic typing on the molecular marker through the flanking sequence, and implementing molecular marker-assisted selection according to the typing result. A reliable way is provided for breeding or cultivating local sheldrake varieties with longer cervical vertebra length, and the breeding process of meat ducks is accelerated.

The invention relates to the technical field of cancer risk prediction, in particular to a deep learning cancer risk prediction method and system based on a methylation sequence. The method comprises the following steps: acquiring DNA methylation sequencing data and reference genome annotation data in a blood sample of a cancer patient; performing functional region classification processing on the DNA methylation sequencing data according to the reference genome annotation data to obtain promoter subregion methylation data, enhancement subregion methylation data and transcription factor binding site methylation data; performing dynamic sequence similarity calculation processing on the methylation data of the promoter region to obtain a first feature vector containing importance weight of the promoter region of the cancer-related gene; the methylation sites are classified according to the functions of the methylation sites in the transcriptional regulation pathway, so that the biological interpretation of the characteristics is improved, and the synergistic effect among different functional regions can be captured.

The invention relates to a single nucleotide polymorphism (SNP) primer of a DSTN gene related to musk ketone content in natural musk and application of the single nucleotide polymorphism (SNP) primer, and belongs to the technical field of biological detection. The invention provides a single nucleotide polymorphism (SNP) primer of a DSTN gene related to the musk ketone content in natural musk, a molecular marker corresponding to the SNP primer is located at a 682371th basic group of a chromosome 23 of a forest musk deer reference genome, the basic group is mutated into A or C, the genotypes are AA and AC, and deoxyribonucleotide sequences of the SNP primer are shown as SEQ ID NO: 1 and SEQ ID NO: 2. According to a breeding target, by eliminating A / A genotype individuals and retaining A / C genotype individuals, the method has the beneficial effects that the relative content character of musk ketone in musk of the forest musk deer is efficiently and quickly identified, and a scientific basis is provided for early breeding of high-quality forest musk deer. The detection method disclosed by the invention is simple and easy to operate, can be carried out in a laboratory, and can also be applied to a genome breeding technology.

The invention provides an ecDNA structure prediction method based on scATAC-seq, a method for identifying cells carrying ecDNA and a medium, and relates to the technical field of biological information. According to the ecDNA structure prediction method based on scATAC-seq, inconsistent readings of ecDNA breakpoint sources are extracted through comparison with a reference genome, enriched peak regions are called in sequence and converted into a graph, a breakpoint graph is combined with a Bayesian model, and accurate prediction of structural information such as ecDNA breakpoints, ecDNA regions and connection modes is achieved. The invention also provides a method for identifying cells carrying the ecDNA, and the method is used for accurately acquiring the cells carrying the ecDNA through standardization treatment, formation of a gene activity matrix and non-single-peak test on the ecDNA structure based on the scATAC-seq. According to the present invention, the ecDNA structure can be predicted, the cells carrying the ecDNA can be accurately identified, and the method can be used for the accurate analysis and research of the tumor heterogeneity, evolution and drug resistance process of the ecDNA.