1231 results about "Reference genome" patented technology

The invention relates to the technical field of molecular biology and molecular markers, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker located on a porcine chromosome 14 and related to porcine reproductive traits and application. The SNP site of the SNP molecular marker located on the No.14 chromosome of the pig and related to the reproductive traits of the pig corresponds to the Cgt at the 105043272th site on the No.14 chromosome of the international pig reference genome version 11.1; t mutation is carried out, and the polymorphism of the basic group at the site affects the porcine reproductive traits; wherein the reproductive performance of pigs with CC and TC genotypes is superior to that of pigs with TT genotypes. By optimizing the dominant alleles of the SNP molecular marker, the frequency of the dominant alleles can be increased generation by generation, the reproductive performance of pigs is improved, and the genetic improvement progress of the pigs is accelerated, so that the economic benefit of pig breeding is effectively improved.

The invention discloses an SNP molecular marker related to the conception rate of young cows, a detection product and application of the SNP molecular marker, and belongs to the technical field of dairy cow breeding markers. The SNP molecular marker is chr6: g. 103355581Cgt, and the SNP molecular marker is a molecular marker. The method comprises the following steps of: taking a bovine reference genome version UMD3.1 as a standard for a coordinate position, numbering Assessment in NCBI (National Center of Biotechnology Information) as GCF000003055.6, and numbering a chromosome as AC 000163.1; the young cows are cows which are 12-24 months old and do not lay calves. By detecting the genotype of the SNP site, the conception rate condition can be effectively judged, parent individuals with the higher young cow conception rate can be screened, early selection of genotype individuals with the high young cow conception rate is achieved, the frequency of the genotype with the high young cow conception rate in a dairy cow group is increased, and the reproduction rate of the dairy cow group is increased; the service life of cows is prolonged, the treatment cost is saved, the breeding cost is reduced, and the pasture income is increased.

The invention belongs to the field of molecular biological identification, and particularly relates to a specific SNP (Single Nucleotide Polymorphism) site combination for identifying a Wuzhishan pig variety and application. The specific SNP site combination for identifying the Wuzhishan pig variety comprises 77 SNP sites, and the physical positions of the SNP sites are determined by sequence comparison based on a pig reference genome Sscrofa11.1. The specific SNP site combination is screened based on a method of combining whole genome association analysis with selection signal analysis so as to ensure the accuracy of site selection. The selected specific SNP site combination can rapidly realize accurate identification of the Wuzhishan pig variety on the gene level, and has significant application value in the aspects of genetic resource accurate protection and variety utilization of Hainan Wuzhishan pigs.

The invention discloses a KASP molecular marker for identifying soybean hundred-grain weight and application thereof, and relates to the technical field of biology and application of an SNP locus, the SNP locus takes a soybean Wm82. A2. V1 genome sequence as a reference genome, the SNP is the 16321243th SNP on a soybean 11 # chromosome, and the SNP is the 16321243th SNP on the soybean 11 # chromosome. Corresponding to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, when the locus is CC homozygosis, the corresponding genotype is A; when the locus is TT homozygous, the corresponding genotype is B; according to the application, soybeans with different hundred-grain weights are screened or screened in an auxiliary mode, and the different hundred-grain weights of the soybeans are as follows: the homozygous soybeans with the genotype A are higher than or candidate to be higher than the homozygous soybeans with the genotype B. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding progeny materials with high hundred-grain weight.

The invention relates to the technical field of crop genetic breeding and plant molecular design breeding, in particular to a pakchoi whole genome liquid phase chip and application. The pakchoi breeding gene chip disclosed by the invention comprises a target capture probe which is designed by taking 1000 core SNP (Single Nucleotide Polymorphism) molecular markers positioned on 40KSNP sites on a pakchoi reference genome version V4.0 as templates. The pakchoi whole genome liquid phase chip provided by the invention comprises a 40K site targeted capture probe mixed solution and a hybridization capture reagent. The pakchoi 40KSNP targeted capture probe can rapidly and effectively track genetic materials of pakchoi, and is suitable for different application scenes such as accurate identification of pakchoi germplasm resources, genetic relationship analysis between materials, variety authenticity identification, QTL gene positioning and mining, molecular marker-assisted breeding, variety improvement and whole genome selective breeding application. Therefore, the method has good application prospect and important social value.

The invention discloses an SNP molecular marker related to the backfat thickness character of a pig living body with the weight of 100 kg and application of the SNP molecular marker. SNP typing data is obtained by integrating re-sequencing data of the pig and comparing the re-sequencing data of the pig to a pig reference genome Sscrofa11.1, whole genome analysis is conducted through a mixed linear model, 5053 SNP sites related to the backfat thickness of the pig living body with the weight of 100 kg are screened out, and the SNP molecular marker related to the backfat thickness character of the pig living body with the weight of 100 kg is obtained. The correlation between the SNP sites and the backfat thickness character of the pig living body with the weight of 100 kg reaches a remarkable level, a new molecular marker is provided for auxiliary selection and improvement of the backfat thickness character of the pig living body with the weight of 100 kg, and the molecular marker has important significance for auxiliary breeding of pigs.

The invention relates to the technical field of molecular biology, and particularly provides an InDel molecular marker related to the size of a lychee kernel and application of the InDel molecular marker. The InDel molecular marker is located at a site 25610680 of a fifth chromosome of a reference genome GeneBank: GCA019925255.1 of the litchi, and the InDel molecular marker is used for detecting the DNA of the litchi. The genotype of the marker in a pyrokaryotic litchi material is homozygous' GAAAAA / GAAAAA ', and the genotype of the marker in a macrokaryotic litchi material is heterozygous' G---- / GAAAAA 'or homozygous' G---- / G----'. The invention further provides a primer group for detecting the molecular marker, an effective tool is provided for molecular marker-assisted breeding of the size of the lychee kernel, and the method is expected to assist in shortening the breeding period, improving the breeding efficiency and promoting the improvement work of the lychee variety.

The invention discloses a tetraploid crassostrea gigas parent-child relationship identification method based on SNP sites, and belongs to the technical field of tetraploid crassostrea gigas molecular assisted breeding. The method for identifying the parent-child relationship of tetraploid crassostrea gigas comprises the following steps: (1) sequencing adductor muscles of tetraploid crassostrea gigas to be detected; (2) comparing the reference genome of the crassostrea gigas to obtain the genotype of the specified SNP site of the tetraploid crassostrea gigas; (3) calculating IBS and LOD of the to-be-detected parents and the to-be-detected offspring according to an SNP typing result; and (4) identifying the parent-child relationship between the to-be-detected parent and the filial generation by combining IBS and LOD. The SNP loci have the advantages that 1000 SNP loci for identifying the parent-child relationship of the tetraploid crassostrea gigas are disclosed for the first time, data support is provided for identifying the parent-child relationship of the tetraploid crassostrea gigas, a foundation is laid for developing a liquid phase chip for identifying the parent-child relationship of the tetraploid crassostrea gigas, and the SNP loci have good application prospects.

Systems and methods for outbreak analysis by performing taxonomic classification of the first LRS data to assign one or more taxonomic identifiers to each record in the first LRS data; identifying a plurality of species-specific candidate genomes from a reference genome database based on the assigned taxonomic identifiers; aligning the first LRS data with each of the plurality of species-specific candidate genomes to determine a first species identity of a microorganism species present in the sample; identifying a first nearest strain of the identified microorganism species present in the first sample by aligning the first LRS data with a plurality of strain-specific candidate genome dataset.

The invention belongs to the technical field of pepper planting, and discloses a pepper multi-branch gene CaBr1 linked KASP molecular marker as well as a primer, a kit and application thereof, the KASP molecular marker takes pepper S8 (zhangshuang) as a reference genome, and the single nucleotide polymorphism at the 103175415th basic group on the No.8 chromosome is subjected to basic group G-to-A replacement. A chromosome region closely linked with multiple branches of the pepper is obtained by using a BSA (Bovine Serum Albumin) population positioning method, a molecular marker is developed in a candidate region to determine a candidate gene CaBr1, a KASP molecular marker is screened according to base mutation of the candidate gene, and the marker is used for carrying out genotype identification on 90 randomly sampled single plants of an F2 population, and the coincidence rate reaches 100%. The result not only contributes to pepper branch phenotype identification and assistant breeding, but also lays a foundation for map-based cloning of the branch gene and analysis of a molecular mechanism of pepper plant type regulation.

The invention relates to a molecular marker closely linked with a pepper fruit length regulation gene CaFL1 and application of the molecular marker. The molecular marker is insertion / deletion of three basic groups GAC at 223149123-223149125th positions of chromosome 10 of a pepper genome, and the position of the molecular marker in the genome is determined according to a Qieme reference genome. Specifically, when the genotype of the molecular marker is InGAC: InGAC, the pepper has a long fruit phenotype, when the genotype is InGAC: DelGAC, the pepper has an intermediate phenotype, and when the genotype is DelGAC: DelGAC, the pepper has a short fruit phenotype. Through verification, the accuracy rate of the molecular marker reaches 100%, early, massive and accurate prediction and directional improvement of the pepper fruit length can be realized, and a new way is provided for screening and identifying the pepper fruit length and breeding the pepper fruit length.

The invention discloses a biological complex trait key gene identification method, device and equipment and a medium, and the method comprises the following steps: obtaining biological reference genome data, and carrying out orthologous gene class group division on a protein sequence coded by the biological reference genome data; determining a characteristic value data set according to the number of the subgroup sequence and the gene number of the subgroup sequence obtained after division; obtaining each piece of biological phenotype data; utilizing a machine algorithm model to construct a phenotype matrix according to the biological phenotype data and the characteristic value data set so as to train a phenotype prediction model, and determining the influence weight of each subgroup sequence on the biological phenotype according to the phenotype prediction model; and according to each influence weight, determining a key gene for regulating and controlling the biological complexity. The method provided by the invention can realize more accurate, efficient and comprehensive identification of key genes of prokaryotes and high biological complexity characters.

The invention relates to the technical field of crop molecular genetic markers, and discloses an InDel molecular marker of avena nuda and application thereof.The molecular marker is a group of 314bp deletion variation and 2bp insertion variation in the 610439484-610440026 interval of the 5C chromosome position of a reference genome of an avena nuda variety OT3098, the nucleotide sequence of the deletion variation is shown as SEQ ID NO.1, the nucleotide sequence of the insertion variation is shown as SEQ ID NO.2, and the nucleotide sequence of the insertion variation is shown as SEQ ID NO.3. The nucleotide sequence of the insertion variation is 5 '-AA-3'. The molecular marker provided by the invention can realize stable detection in agarose gel electrophoresis by combining a specific primer pair (SEQ ID NO.2-3) with an optimized touchdown PCR (Polymerase Chain Reaction) program, has the advantage of being not influenced by a tissue part and a growth period, and provides an efficient and reliable technical means for oat germplasm identification, seed purity detection and molecular breeding.

The invention discloses a KASP molecular marker for identifying soybean protein content and application, and relates to the technical field of biology and application of an SNP locus, the SNP locus takes a soybean Wm82.a2.v1 genome sequence as a reference genome, is the 42148495th SNP on a soybean chromosome 9 and corresponds to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, and when the locus is TT homozygosis, the corresponding genotype is A; when the site is AA homozygous, the corresponding genotype is B; according to the application, different protein contents of soybeans are screened or screened in an auxiliary mode, and the different protein contents of the soybeans are as follows: the homozygous soybeans of the genotype A are higher than or candidate to be higher than the homozygous soybeans of the genotype B. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding offspring materials with high protein content.

The invention discloses a KASP molecular marker for identifying the soybean oil content and application, and relates to the technical field of biology and application of an SNP locus, the SNP locus takes a soybean Wm82. A2. V1 genome sequence as a reference genome, the SNP is the 6562032th SNP on a soybean 20 # chromosome, and the SNP is the 6562032th SNP on the soybean 20 # chromosome. Corresponding to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, when the site is GG homozygosis, the corresponding genotype is A; when the site is AA homozygous, the corresponding genotype is B; according to the application, soybeans with different oil contents are screened or screened in an auxiliary mode, and the different oil contents of the soybeans are as follows: the soybean with the homozygous genotype A is higher than the soybean with the homozygous genotype B or the soybean with the homozygous genotype B in a candidate mode. The invention has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding progeny materials with high oil content.

The invention relates to the technical field of cancer risk prediction, in particular to a deep learning cancer risk prediction method and system based on a methylation sequence. The method comprises the following steps: acquiring DNA methylation sequencing data and reference genome annotation data in a blood sample of a cancer patient; performing functional region classification processing on the DNA methylation sequencing data according to the reference genome annotation data to obtain promoter subregion methylation data, enhancement subregion methylation data and transcription factor binding site methylation data; performing dynamic sequence similarity calculation processing on the methylation data of the promoter region to obtain a first feature vector containing importance weight of the promoter region of the cancer-related gene; the methylation sites are classified according to the functions of the methylation sites in the transcriptional regulation pathway, so that the biological interpretation of the characteristics is improved, and the synergistic effect among different functional regions can be captured.

The invention relates to a single nucleotide polymorphism (SNP) primer of a DSTN gene related to musk ketone content in natural musk and application of the single nucleotide polymorphism (SNP) primer, and belongs to the technical field of biological detection. The invention provides a single nucleotide polymorphism (SNP) primer of a DSTN gene related to the musk ketone content in natural musk, a molecular marker corresponding to the SNP primer is located at a 682371th basic group of a chromosome 23 of a forest musk deer reference genome, the basic group is mutated into A or C, the genotypes are AA and AC, and deoxyribonucleotide sequences of the SNP primer are shown as SEQ ID NO: 1 and SEQ ID NO: 2. According to a breeding target, by eliminating A / A genotype individuals and retaining A / C genotype individuals, the method has the beneficial effects that the relative content character of musk ketone in musk of the forest musk deer is efficiently and quickly identified, and a scientific basis is provided for early breeding of high-quality forest musk deer. The detection method disclosed by the invention is simple and easy to operate, can be carried out in a laboratory, and can also be applied to a genome breeding technology.

The invention provides an ecDNA structure prediction method based on scATAC-seq, a method for identifying cells carrying ecDNA and a medium, and relates to the technical field of biological information. According to the ecDNA structure prediction method based on scATAC-seq, inconsistent readings of ecDNA breakpoint sources are extracted through comparison with a reference genome, enriched peak regions are called in sequence and converted into a graph, a breakpoint graph is combined with a Bayesian model, and accurate prediction of structural information such as ecDNA breakpoints, ecDNA regions and connection modes is achieved. The invention also provides a method for identifying cells carrying the ecDNA, and the method is used for accurately acquiring the cells carrying the ecDNA through standardization treatment, formation of a gene activity matrix and non-single-peak test on the ecDNA structure based on the scATAC-seq. According to the present invention, the ecDNA structure can be predicted, the cells carrying the ecDNA can be accurately identified, and the method can be used for the accurate analysis and research of the tumor heterogeneity, evolution and drug resistance process of the ecDNA.

The invention belongs to the field of molecular biology, and discloses acquisition of a waterlogging-resistant major QTL qWT1.1 in a corn seedling stage and development and application of a molecular marker primer of the waterlogging-resistant major QTL qWT1.1. The invention provides a major QTL qWT1.1 for controlling the waterlogging survival rate in the corn seedling stage, the major QTL qWT1.1 is located at the fifth version physical position of a reference genome in the first staining of the corn, the fifth version physical position of the reference genome is Chr1: 25904794-27039204, the major QTL site is used for controlling waterlogging-resistant phenotypic variation, an excellent haplotype SNP marker closely linked with the major QTL site is located at the 27039148th basic group of a first chromosome of a reference genome of the corn B73, and the excellent haplotype SNP marker is located at the 27039148th basic group of a second chromosome of the reference genome of the corn B73. The explained waterlogging survival rate phenotypic contribution rate is 10.03%, the PARMS marker designed by the SNP is used for detecting a corn inbred line group, the operation is simple and convenient, the typing is clear, the good selection effect on characters such as root length, root number, plant height, branch number and yield under the stress of corn waterlogging is realized, and the application prospect is wide. The molecular marker can be used for molecular marker-assisted selective breeding of the waterlogging resistance character in the corn seedling stage.

The invention discloses a method for assisting in identifying the feed conversion ratio of pigs and a molecular marker thereof, and belongs to the technical field of biology. The method comprises the following steps: detecting the genotype of an SNP (Single Nucleotide Polymorphism) site in a pig genome to be detected, and according to the feed conversion ratio of the genotype pig, the SNP site is located at the 3714623rd site of a No.17 chromosome in a pig reference genome (Susscrofa.Sscrofa11.1), and the No.17 chromosome in the pig reference genome (Susscrofa.Sscrofa11.1) is shown as SEQ ID NO: 1. The method is of great significance to selection of pig breeds with low feed conversion ratio.

The invention relates to application of a molecular marker related to daily gain of pigs at the stage of 30kg-120kg. The invention discloses a method for identifying the daily gain of pigs at the stage of 30 kg to 120 kg and a breeding method for breeding the pigs with the daily gain at the stage of 30 kg to 120 kg. According to the method, by detecting the genotype of a to-be-detected pig at the 161610871th site of a chromosome 1 in a pig reference genome Susscrofa.Sscrofa11.1, the daily gain of the pig at the stage of 30 kg to 120 kg is identified, the pig with the TT genotype is higher than the pig with the CT genotype, and the pig with the CT genotype is higher than the pig with the CC genotype. In actual breeding work, a to-be-detected pig with the genotype of the C161610871T site being TT can be selected as a parent for breeding, and the method has important significance on selection of pigs with high feed intake and high feed utilization efficiency.

The invention belongs to the technical field of molecular markers, and discloses a molecular marker located in No.18 chromosome and related to the salt tolerance of soybean seeds in the germination period and application of the molecular marker. According to the present invention, the salt tolerance index of the soybean seed germination stage is determined, the stable and reliable molecular marker GmChr1856968562 closely linked with the salt tolerance ability of the seed germination stage is obtained through GWAS correlation analysis, the molecular marker is located at the 56968562 site on the No.18 chromosome of the soybean reference genome Glycine max Wm82. A2. V1, and the marker for KASP detection is developed based on the site, the invention also designs a primer group for amplifying the KASP marker. The invention further provides a method for rapidly identifying the salt tolerance of the soybean seeds in the germination period by using the primer group of the molecular marker, and the method is simple, convenient and rapid, accurate in identification result and good in popularization and application prospect.

The invention discloses a low-abundance pathogen detection method and system based on metagenome sequencing data, and belongs to the technical field of bioinformatics. The system comprises a species specificity sequence screening module (BactAlert2SSRs), a reference genome database construction module (BactAlert2Lib), a sequence alignment reference genome module (BactAlert2Map), a sequence alignment result analysis module (BactAlert2ID), and an SSRs posterior probability correction module (BactAlert2Rec). The detection system realizes efficient, accurate and reliable detection of low-abundance pathogens, and is suitable for analyzing third-generation metagenome sequencing data.

The invention relates to the technical field of molecular detection, and particularly discloses a liquid chip for identifying opisthopapus taihangensis and Hebei province as well as a preparation method and application of the liquid chip. Genotyping sites of the liquid chip comprise 1140 SNP sites; the physical positions of the 1140 SNP loci are determined based on comparison of a whole genome sequence of a bovine reference genome, and the version number of the whole genome sequence of the bovine reference genome is ARSUCD 1.2; wherein the physical position information of the 1140 SNP loci is as shown in a table 1. The liquid chip can realize genetic typing of the opisthopapus taihangensis and the Hebei province cattle, has the advantage of high detection rate, and can obviously cluster the opisthopapus taihangensis and the Hebei province cattle into two classes when clustering analysis is carried out on the opisthopapus taihangensis and the Hebei province cattle, so that a marker combination of the liquid chip can represent whole genome re-sequencing genes, assists in distinguishing the opisthopapus taihangensis and the Hebei province cattle, and is high in accuracy and high in accuracy. Therefore, the variety identification of the opisthopapus taihangensis and the Hebei Hebei is realized.

The invention relates to an SNP molecular marker related to litchi fruit weight and application thereof, and belongs to the technical field of molecular biology. The SNP molecular marker related to the litchi fruit weight is based on a seedless litchi reference genome, the SNP molecular marker is located in a litchi SL05G0138630 gene coding region, and is located at the 27564818th basic group of a litchi chromosome 5, and the basic group is A or G. The application finds that a non-synonymous SNP site in a coding region of the litchi SL05G0138630 gene is closely associated with the litchi fruit weight phenotype, the variety with the weight of the litchi fruit can be efficiently identified by detecting the polymorphic site, the invention further discloses an identification method for the molecular level of the variety with the weight of the litchi fruit, and the molecular level of the variety with the weight of the litchi fruit can be identified by an SNP-based typing method. And cheap and accurate gene SNP typing can be achieved, so that the purpose of efficiently and accurately identifying the weight variety of the litchi fruit is achieved.

The invention relates to the technical field of bioinformatics data processing, in particular to a genome sequence compression method and system. The method comprises the following steps: acquiring genome sequencing data; comparing the sequencing data with a reference genome to determine a difference site; differentiating the difference sites as sequencing errors or real variations through a time sequence difference neural network model; performing differential compression coding according to an identification result; a friendly variation detection format is constructed, and rapid variation query is supported through a multi-level index structure and a variation metadata table. According to the method provided by the invention, the sequencing error and the real variation can be accurately distinguished through the time sequence differential neural network model, and important biological variation information is protected while the compression efficiency is improved by adopting the differential compression coding strategy.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker closely linked with a sweet corn kernel weight major QTL (Quantitative Trait Loci) and application of the SNP molecular marker, and belongs to the technical field of SNP molecular markers. The nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO.1, the mutation site is the base of the 242797157th site on the first chromosome of a B73 reference genome (RefGenv4), the base of the site is T, and the sweet corn with the genotype of TT shows the high grain weight character; the basic group of the site is C, and the sweet corn with the genotype of CC shows the character of low grain weight. Based on the SNP molecular marker, a method for identifying the sweet corn grain weight character and a method for screening the sweet corn with the high grain weight character are provided, and the methods are high in accuracy, high in detection speed, easy in result interpretation and low in cost and have important significance in improvement of corn breeding efficiency and large-scale high-yield planting of corn.

The invention discloses a muskmelon fruit length molecular marker and application thereof. According to the method, two methods of BSA-seq and GPS-seq are utilized to perform preliminary positioning on the muskmelon fruit length related QTL, a reference genome Mel (DHL92) gene 3.6. 1 and a re-sequencing result are utilized to perform comparative analysis, two algorithms of SNP-index and InDel-index are comprehensively utilized to perform correlation analysis, the results of the two analysis methods are respectively counted, and the results of the two analysis methods are compared with those of the QTL. The larger segment is used as a candidate region associated with the length of the melon fruit, KASP06-3880 and KASP06-3606 are obtained, and the KASP06-3880 is located at the 34305438th basic group of the No.6 chromosome; the KASP06-3606 is located at the base at the 18936169th site of the chromosome 11; the two KASP markers KASP06-3880 and KASP11-3606 are subjected to genotype detection in extreme materials of F2 and RIL populations, and the two KASP markers determine the length of the muskmelon fruits, so that a new way is provided for breeding of the muskmelon fruits with different lengths.

The invention discloses an SNP (Single Nucleotide Polymorphism) and InDel site combination for identifying variety of Inner Mongolia cattle, a 5K targeted capture gene chip and application of the 5K targeted capture gene chip. The SNP and InDel site combination for identifying the variety of the Inner Mongolia cattle is firstly disclosed and comprises 5000 SNP and InDel sites, and the physical position information of a cattle reference genome with the version number of AR-UCD1.2 is shown in a table 1. The invention further discloses a 5K targeting capture gene chip comprising the probe targeting the SNP and InDel site combination as well as a synthesis method and application of the 5K targeting capture gene chip. The 5K targeted capture gene chip completes genetic typing through a targeted capture probe by means of a high-throughput sequencing technology, has the characteristics of low cost, high efficiency and high accuracy, and can realize the variety identification function of Mongolian cattle, Sanhue cattle and Sunitt cattle by utilizing genotype data; important technical support and specific means are provided for identification and evaluation of Inner Mongolia cattle germplasm resources.

The invention discloses an InDel marker closely linked with the sweet-glutinous homograin character of corn ears, a corn B73-V4 genome is used as a reference genome, the marker is a <-> / insertion sequence of 73 bp located at the position of 12126328 bp of a No.9 chromosome, and the nucleotide sequence of the insertion sequence is as shown in SEQ ID NO.1. The InDel marker closely linked with the sweet-glutinous homograin character of the corn ears has the advantages that the InDel marker closely linked with the sweet-glutinous homograin character of the corn ears is obtained; the InDel marker provided by the invention can be used for rapidly screening out a corn material containing a sweet-waxy homograin gene in the early growth stage of corn, and can also be used for molecular marker-assisted breeding of fresh corn. By adopting the InDel marker and the screening method provided by the invention, the screening accuracy is high, and by using the marker and the screening method, the production cost is saved, and the breeding efficiency is improved.