469 results about "Gene sequence" patented technology

The invention discloses L-arabinose isomerase, engineering bacteria and application, the amino acid sequence of the L-arabinose isomerase is shown as SEQ ID NO: 3, and the gene sequence for coding the L-arabinose isomerase is shown as SEQ ID NO: 2. On the basis of a genome of a Peribacillussp.S4 strain, a gene for coding the L-arabinose isomerase is excavated, and a stable prokaryotic expression system is constructed by performing codon optimization on the gene, so that the large-scale controllable production of the L-arabinose isomerase is realized, the yield of the enzyme is remarkably increased, the production cost of the enzyme is reduced, and the method is suitable for industrial production. A stable and economical enzyme source is provided for the production of D-tagatose; the L-arabinose isomerase expressed by the constructed engineering bacteria can significantly improve the yield of D-tagatose, and industrialization of D-tagatose is facilitated.

The invention belongs to the technical field of essential gene prediction, and particularly relates to an essential gene prediction method based on DNA large model and time-frequency domain deep learning fusion, and the method comprises the steps: taking a domain DNA large model as a core representation layer, and obtaining special gene representation through cross-species corpus pre-training and task fine tuning; a T-Block and F-Block dual-channel time-frequency fusion structure is adopted, and the local dependence and long-range regulation relation of a gene sequence is synchronously captured by expanding DFT (Discrete Fourier Transform), complex value attention and iDFT (Initial Discrete Fourier Transform) conversion; designing an efficient modeling reasoning scheme of sliding window slices and gene-level aggregation aiming at an ultra-long sequence; in combination with class imbalance and a noise robust training strategy, cross-cell line / cross-platform transferable threshold output is realized through temperature scaling calibration, an uncertainty quantization and structured interface is matched, and drug target screening and experimental design decision are supported. The system supports the realization of multiple programming languages, and can complete low-delay end-to-end reasoning in a conventional hardware environment.

The invention provides a valine production strain and a construction method and application thereof, a designed acetohydroxy acid synthase mutant is that the 88th basic group of an ilvB gene is changed from a to c, the 382nd basic group is changed from a to g, the 413th basic group is changed from c to t, the gene sequence of a designed artificial operon comprises a promoter, an ilvB (A138V) gene or ilvB (Q30K, S128G, A138V) gene of coding mutated acetohydroxy acid synthase, and an ilvN (G20D, I21D, I21D, I21D, I21D, I21D, I21D, I21D) gene. I22F) gene, a pyk gene for coding pyruvate kinase, and a terminator; by designing a specific acetohydroxyacid synthase mutant and related biological materials and artificial operon, the strain constructed by directional modification of the strain by using a pK18mobsacB system gene editing technology based on allele exchange has the advantages of good genetic stability, high fermentation yield and the like, and valine can be stably produced.

The invention discloses a crop ralstonia solanacearum disease prediction method based on an integrated learning model, and the method comprises the following steps: S1, collecting a published 16s rRNA gene sequence related to solanaceae crop bacterial wilt, and carrying out the preprocessing of original sequencing data based on an EasyAmplicon standardized process; s2, performing data dimension reduction by using a principal component analysis algorithm, and retaining 95% of variance; s3, performing hyper-parameter search based on 5-fold cross validation and grid search on the Light GBM model, the CatBoost model and the XGBoost model respectively, and selecting three groups of optimal hyper-parameters of each model; s4, constructing a model according to three groups of optimal hyper-parameters of each selected model, performing prediction, analyzing model result difference based on a Pearson correlation coefficient, and retaining Pearson correlation coefficient mean < lt > with other eight model prediction values; a model of 0.8; and S5, inputting the screened model prediction result into a second-layer element learner RF for integrated learning to obtain a final prediction result.

The invention relates to a recombinant expression cat interferon-omega gene and a preparation method and application thereof, the nucleotide sequence of the cat interferon-omega gene is as shown in SEQ ID NO.1, and the cat interferon-omega gene is obtained by introducing an Fc fusion fragment to the C terminal and / or N terminal of the natural gene sequence of the cat interferon-omega. XTEN or PAS is introduced to prolong a peptide fragment, and original glycosylation modification sites on a natural sequence are reserved. The method comprises the following steps: cloning a cat interferon-omega gene into an expression vector containing double promoters, further transfecting into a cell, carrying out stable cloning and screening, establishing a high-expression cell strain, and carrying out fermentation culture in a bioreactor. According to the invention, the cat interferon-omega gene is subjected to multiple modification and is efficiently expressed in a CHO-K1GS system, so that the protein yield, the stability and the half-life period are remarkably improved; the obtained fusion protein is high in purity and strong in activity, can obviously enhance immune response and protection effect when being matched with cat vaccines, and is good in safety.

The invention discloses an allergen specific IgE and IgG antibody composite quality control product or calibration product as well as a preparation method and application thereof, and belongs to the technical field of biological detection. The preparation method comprises the following steps: obtaining a whole-genome sequence comprising a human IgE constant region gene sequence, a human IgG4 constant region gene sequence and a human IgG1 constant region gene sequence, and preparing a fusion protein simultaneously containing a human IgE Fc segment and a human IgG Fc segment; immunizing a healthy animal by using the allergen to obtain a purified antibody; and carrying out chemical coupling on the purified antibody and the fusion protein to obtain allergen specific IgE and IgG antibodies. By constructing allergen specific IgE and IgG antibodies, the kit can be simultaneously applied to detection of allergens IgE and IgG, has the advantages of high titer, high purity, good dilution linearity and the like, and is beneficial to improvement of diagnosis accuracy and treatment effectiveness.

The invention provides application of Ustilaginoidea virens secretory protein UvWMT and a coding gene thereof in prevention and control of plant diseases. The gene sequence of the Ustilaginoidea virens secretory protein UvWMT is researched, found and cloned for the first time, and the new application of the Ustilaginoidea virens secretory protein UvWMT in disease-resistant breeding of crops such as rice is developed. Research finds that the protein is a key pathogenic factor in the process that rice is infected by ustilaginoidea virens, heterologous expression experiments further find that heterologous expression of UvWMT in rice can significantly improve the resistance of crops to various pathogenic bacteria such as ustilaginoidea virens, magnaporthe oryzae and xanthomonas oryzae, and has no significant difference in key agronomic traits compared with a wild type. An important theoretical basis is provided for rice disease-resistant breeding, crops can be safely and effectively endowed with broad-spectrum disease resistance by heterologous expression of the gene, and the gene has great application prospects and economic values in the aspects of crop breeding disease resistance improvement, rice disease prevention and control and crop yield increase and pesticide reduction.

The invention discloses a primer for diagnosis of early pregnancy of sows. The nucleotide sequence of the primer is shown as follows: SRY-O-F: 5 '-CAGCAAAATATTCTCGCCTTGG-3', and the nucleotide sequence of the primer is shown as follows. And SRY-O-R: 5 '-CATCCTCTCTCTACGC-3', and SRY-O-R: 5 '- SRY-I-F: 5 '-ATATTCTCGCCTTGGGG-3', SRY-I-F: 5 '- And SRY-I-R is 5 '-GCTTTCGGCTTCTGTA-3', and the formula is shown in the description. Two pairs of primers with high sensitivity and strong specificity are designed through comparative analysis of pig SRY gene sequences, fetal free DNA in peripheral blood of sows is used as a template, and the specificity and sensitivity of PCR are enhanced through amplification of the two pairs of primers. Through two rounds of PCR amplification, whether the sow is pregnant or not can be detected on the 18th day of pregnancy, and the accuracy of the detection result is extremely high and reaches 100%. Compared with the prior art that the ZFY gene is used for detecting the sows on the twentieth breeding day, the method has the advantages that the detection time can be advanced to the eighteenth breeding day, and the detection accuracy is greatly improved (100% vs 10%). According to the method, powerful technical support is provided for early and accurate identification of the non-pregnant sows, reduction of non-production days and feeding cost and smooth execution of a production plan.

The invention discloses a vector system and method capable of converting mouse embryonic stem cells into embryonic-like cells in a 2-cell period, the vector system comprises a mouse Zfp352 gene sequence, and the nucleotide sequence of the mouse Zfp352 gene is shown as SEQ ID No.1. The invention also discloses a method for converting the mouse embryonic stem cells into embryonic-like cells in a 2-cell period. The invention creatively discovers that through overexpression of the Zfp352 in the mouse embryonic stem cells, the efficiency of converting the mouse pluripotent embryonic stem cells into the totipotent embryonic-like cells in the 2-cell period can be remarkably improved.

The invention belongs to the technical field of bioengineering, and discloses a genetic engineering strain for enhancing schizochytrium limacinum DHA oil production based on carbon flux guidance and fatty acid transport, a method and application thereof. The gene engineering strain is obtained by introducing an optimized coding gene ACLY and / or Fat1p coding gene related to guiding carbon flux and fatty acid transport into a schizochytrium limacinum body; wherein the gene sequence of the gene ACLY is SEQ ID No. 9, and the gene sequence of the gene Fat1p is SEQ ID No. 10. According to the invention, a schizochytrium limacinum engineering bacterium for enhancing DHA oil production based on carbon flux guidance and fatty acid transfer is constructed by means of metabolic engineering. The schizochytrium limacinum engineering bacterium is combined with fatty acid transfer based on carbon flux guiding, can be used for grease synthesis by guiding carbon flux, and can prevent grease from being degraded through fatty acid transfer.

The invention relates to the technical field of interface calling, and provides an interface scheduling optimization method and device, equipment, a storage medium and a program product, and the method comprises the steps: carrying out the gene coding of each to-be-called application interface, and obtaining the gene sequence of each application interface; the gene sequence corresponds to a scheduling scheme of the application interface; generating a population based on the gene sequence, and constructing a fitness function according to the evaluation index of the application interface; the evaluation indexes comprise a bandwidth utilization rate, a calling success rate and response duration; performing iterative optimization on the population based on the fitness function by adopting an improved genetic programming algorithm to obtain an optimal individual; and performing scheduling optimization on the application interface based on the target scheduling scheme corresponding to the optimal individual. Through gene coding and an improved genetic programming algorithm, intelligent optimization of an interface scheduling scheme is realized, manual intervention is reduced, optimization efficiency and accuracy are improved, and an optimization effect is ensured.

This invention belongs to the fields of locust biotechnology and gene editing technology, specifically relating to a method for preparing locusts. Crz Methods using gene mutants and their application in locust control. This invention discovers that inhibiting gene mutants in locusts... Crz Gene expression can be used to control locusts. Crz The gene sequence is shown in SEQ ID NO:3. This invention successfully established a homozygous Crz mutant strain of the migratory locust using gene editing technology. It was found that the albino migratory locusts obtained after Crz deletion were more easily preyed upon by natural enemies than the wild-type locusts, exhibited significantly lower levels of the aggregation pheromone 4VA, and showed weaker adaptability to low temperatures compared to the wild type. This provides a new strategy for the biological control of migratory locusts and can also serve as a reference for the biological control of other pests.

This invention relates to a rapid identification method Lactobacillus plantarum The method for identifying strains, belonging to the field of species and strain identification, is based on the differences in three DNA barcode sequences. Lactobacillus plantarum CGMCC NO: 26171 strain from Lactobacillusplantarum This method allows for rapid identification from other strains of the same species. Compared to traditional morphological identification methods, the standard gene sequence obtained is beneficial for the molecular identification of *Lactobacillus plantarum* strains, effectively shortening the identification time. Three pairs of DNA barcoding primers enable specific amplification of the test strain, allowing for rapid identification. Lactobacillus plantarum strains.

The application belongs to the technical field of biotechnology, and particularly relates to a porcine epidemic diarrhea virus recombinant strain, a construction method and application. The S gene sequence of the existing epidemic porcine epidemic diarrhea virus PEDV CH / SX / 2016 strain is replaced by the S2015 gene sequence shown in SEQ ID NO. 1 to construct a PEDV CH / SX / 2016 strain infectious clone. A porcine epidemic diarrhea virus recombinant strain rCH / SX / 2016-S 2015 is successfully rescued by transfecting cells with a plasmid containing the infectious cDNA shown in SEQ ID NO. 4. The problem that the original strain cannot be successfully rescued by reverse genetics engineering is solved, and the recombinant virus strain rCH / SX / 2016-S 2015 has an activity comparable to that of the original strain, and can be used for preparing a PEDV diagnostic reagent and / or vaccine.

This invention belongs to the field of genetic engineering technology, specifically involving GmDREB2A2 Application of genes in regulating fatty acid content in plants. Overexpression. GmDREB2A2 Gene regulation of fatty acid content in plants. This invention utilizes an exogenous gene sequence expression vector to express the coding sequence shown in SEQ ID NO.1. GmDREB2A2 Gene sequences are introduced into plants to enable GmDREB2A2 Gene overexpression. Under these conditions, the plant's linolenic acid, linoleic acid, and palmitic acid content were significantly higher than the control, while the content of eicosatrienoic acid and erucic acid was significantly reduced.

The application discloses two kinds of small RNA compositions for improving biological control effect of termites, and microRNA-like RNA (milRNA) sequences are milR-32 and milR-48 respectively. Double-stranded RNA can enhance the effect of the beetle green fungus on killing termites. The milR-32 and the milR-48 belong to the milRNA coded by the green fungus, and the gene sequences are derived from the transcriptome database of the beetle green fungus. The milR-32 and the milR-48 mimics are designed and synthesized based on the milR-32 and the milR-48 sequences respectively. After the beetle green fungus is used to infect the termites taking the milR-32 or the milR-48 mimic, the infection mortality of the termites is significantly increased. The mimic designed based on the milR-32 and the milR-48 sequences can significantly improve the biological control effect of the termites.

Nucleotide compositions, recombinant vectors, and recombinant viral genomes are disclosed that include a modified horseradish peroxidase (HRP) gene sequence, as well as recombinant viruses that have the modified HRP gene sequence incorporated into the viral genome thereof. The recombinant viruses are utilized in an HRP-based assay to analyze the neutralization potential of candidate antiviral agents, such as antiviral antibodies.

The invention discloses a protein capable of inducing a plant immune activation function and application of the protein in disease control, the gene sequence of the protein capable of inducing the plant immune activation function is shown as SEQ ID NO: 1, and the amino acid sequence of the protein is shown as SEQ ID NO: 2. According to the invention, a novel plant immune activator protein CgSsp16 secreted by colletotrichum gloeosporioides is identified, can be identified by plants, stimulates a plant immune response mechanism, triggers broad-spectrum resistance of host plants to various pathogens and improves disease resistance of various plants; the compound is expected to be developed into a novel biopesticide to be applied to green and sustainable production of agriculture.

The present application relates to the field of molecular biology, in particular to a primer probe composition, kit and detection method for 23 human papilloma virus genotyping detection, wherein the hydrolysis probe and molecular beacon probe are respectively designed in two different regions of HPV gene sequence E6 and L1, and the fluorescence collection point in the amplification stage is set in the high-temperature denaturation stage, so that the detection in the amplification stage and the melting curve stage is independent and does not interfere with each other, thereby realizing the combined use of real-time fluorescent PCR amplification detection technology and melting curve technology; the technology improves the single-well detection target flux of fluorescent PCR analysis technology, reduces the detection cost, and provides a more optimal detection method for HPV multiplex fluorescent PCR genotyping detection.

A glucosyltransferase catalyzing RA to produce RM and a use thereof are provided. The glucosyltransferases derived from Cornus florida and Juglans regia L. are screened and obtained. It was found that the glucosyltransferase derived from the Cornus florida exhibited a relatively high enzymatic activity in catalyzing the RA to produce the RD, while the glucosyltransferase derived from the Juglans regia L. exhibited a relatively high enzymatic activity in catalyzing the RD to produce the RM. When these two enzymes were used together in catalyzing the RA to produce the RM, a relatively high conversion rate was achieved. The use of the glucosyltransferases derived from the Cornus florida and the Juglans regia L. for preparing the RM of SGs, has not been reported yet. The glucosyltransferase provides an excellent candidate protein resource and its corresponding gene sequence for the enzymatic conversion process of the SGs.

The invention relates to the technical field of plant genetic engineering, and discloses a strawberry stolon regulation gene ZFP6 and application thereof, and the nucleotide sequence of the gene is as shown in SEQ ID NO.1. Researches prove that the ZFP6 gene is specifically expressed in axillary meristem of strawberry in the adult-plant stage, and the GA20ox4 gene can be directly or indirectly activated to start a biosynthesis pathway of GA and drive axillary buds to develop towards stolons. Through genetic interaction analysis of the ZFP6 gene, the GA20ox4 gene and the RGA1 gene, the function of the ZFP6 gene as an axillary bud fate decision upstream regulation factor is further verified, the blank of an endogenous regulation mechanism of the strawberry stolons is filled, and the ZFP6 gene is proved to be a molecular target for specifically regulating formation of the strawberry stolons. The gene sequence, the primer, the gene knockout expression vector and the matched technical system provided by the invention lay a foundation for strawberry molecular breeding and industrial application.

The invention relates to the technical field of genetic engineering and molecular breeding, in particular to a ramie MYB transcription factor and application thereof. The MYB transcription factor is BnMYB6, the amino acid sequence of the MYB transcription factor is as shown in SEQ ID NO.1, and the gene sequence for coding the amino acid of the MYB transcription factor is as shown in SEQ ID NO.2. The transcription factor protein BnMYB6 can specifically and negatively regulate the biosynthesis of plant lignin, and has application value in the aspects of inhibiting the synthesis of plant lignin, promoting the growth and development of a root system and maintaining related traits such as the activity of the root system under drought stress.

This invention provides a method and kit for enhancing the yield and quality of gene synthesis products, comprising the following steps: providing a total primer mixture, which includes multiple oligonucleotide single strands encoding a target gene sequence / encoding an operational sequence and sterile water; using the primer mixture as a template, performing the first... PCR The reaction yielded the primary spliced ​​product; a second reaction was then performed using start and end primers. PCR The reaction yielded the crude gene synthesis product; nested primers were then used for a third reaction. PCR The reaction yields a nested structure. PCR Products; nested PCR The product was subjected to agarose gel electrophoresis, and the target band was excised and recovered. This application improves upon traditional gene synthesis methods by adding operation sequences to both ends of the synthesized gene sequence and utilizing highly specific nested primers for nested gene synthesis. PCR It can synthesize more than 2000bp in one go. DNA Double-stranded sequences significantly improve the yield and quality of gene synthesis products.

The invention relates to a RAA-CRISPRCas13a method for detecting iridovirus fluorescence of micropterus salmoides, and relates to the technical field of biological detection.The method comprises the following steps that an RAA primer pair and guide crRNA are designed and synthesized based on a virus specific gene sequence; the method comprises the following steps: constructing a single-tube reaction system containing an RAA amplification component, Cas13a protein, crRNA and a fluorescent reporter molecule; after sample nucleic acid is added, constant-temperature incubation is carried out for 15-30 minutes at 37-42 DEG C, an RAA amplification product activates trans-cleavage activity of Cas13a, and a cleavage reporter molecule generates a fluorescence signal; and virus judgment is realized by detecting a fluorescence signal. According to the method, isothermal amplification and CRISPR detection are efficiently integrated through a single-tube reaction system, and the method has the advantages of being easy and convenient to operate, rapid in detection, high in sensitivity and high in specificity and is suitable for on-site high-throughput screening.

The application discloses a primer probe set for simultaneously detecting multiple frog viruses, a kit and application thereof, and belongs to the technical field of animal virus detection.The primer probe set for detecting frog viruses comprises a primer group with the sequence shown in SEQ ID NO.1-2 and a probe with the sequence shown in SEQ ID NO.3.According to the D5family NTPase gene sequence of the Ranavirus member, the primer and the probe are designed, and through optimization of reaction conditions, a method for detecting frog viruses based on qPCR is finally established.The method has the characteristics of high specificity, good repeatability, high sensitivity, simple operation and reliability, and has good market application prospect and popularization and application value.

The invention belongs to the technical field of biology, and provides an application of alpha-amylase protein derived from nephroplasmosis in culture of nephroplasmosis, and the alpha-amylase protein is obtained by synthesizing a gene sequence of alpha-amylase as shown in SEQ ID NO.2 after codon optimization and then transfecting the gene sequence to an expression vector. And carrying out bacterial transformation, culture, induction and crushing. The invention also provides a method for inducing the formation of the reproductive cyst of the reniform worm. It is found for the first time that alpha-amylase protein specifically induces reproductive cysts to be formed, the biomass of the nephroplasmosis is increased, the growth generation of the nephroplasmosis is shortened, and the method is suitable for species such as distended nephroplasmosis and nephroplasmosis henslouianum and fits natural ecological scenes; complex equipment is not needed, the cost is low, large-scale production is easy, the stability is high, and the method can be widely applied to soil ecological restoration, agricultural microbial resource development and basic research related to nephroplasmosis.

The application discloses a bocachito Nat10 antibacterial peptide, a coding sequence and use and a preparation method thereof, and relates to a method based on multiomics, a biological big data based on multiomics data of bocachito, screening and identifying an antibacterial peptide precursor gene sequence, and obtaining an amino acid sequence of the bocachito Nat10 antibacterial peptide through translation design, as shown in SEQ ID NO: 3. Through activity simulation prediction and in-vitro experiment verification, the bocachito Nat10 antibacterial peptide shows inhibitory effects on the growth activities of Streptococcus agalactiae, Salmonella enteritidis, Staphylococcus aureus, Vibrio anguillarum and Escherichia coli, especially on Staphylococcus aureus, and shows the best antibacterial effect at a concentration of 2.5 micromoles per liter. The bocachito Nat10 antibacterial peptide can be used for preparing various bactericidal preparations, including bacteriostatic agents, medicines or additives, and has good practical value and social ecological benefits.

The invention discloses an RNAi (Ribonucleic Acid Interfere) target gene for efficiently killing water borers and application of the RNAi target gene. According to the application, a recombinant expression vector capable of expressing and targeting dsRNA of the gene and recombinant escherichia coli containing the recombinant expression vector are constructed based on a Br-C gene sequence of the water borers. The dsRNA aiming at the target gene of the chilona arenaria is expressed in Escherichia coli, RNAi reaction in the chilona arenaria is induced after feeding, the target gene is down-regulated, so that the high fatality rate of the chilona arenaria can be achieved, the insecticidal method is environment-friendly and low in price, the problem that the chilona arenaria is lack of insecticidal dsRNA at home and abroad at present is solved, and the method has a wide application prospect. The method can be subsequently used for research and development of novel insecticides.

The invention provides a primer, a probe and a kit for identifying coreius guichenoti based on environmental DNA, and a specific primer and a probe designed according to a COI gene sequence in coreius guichenoti mtDNA, namely the primer and the probe can specifically identify coreius guichenoti in numerous fishes, so that the detection time is greatly shortened, the detection can be completed within 6 hours, and the detection efficiency is greatly improved. The method is simple in operation, accurate in monitoring result and high in sensitivity, the lowest DNA concentration of real-time fluorescence PCR detection can reach the pg level, and the method better meets the actual requirements of eDNA field detection in the upstream of the Yangtze River.

This invention discloses a gene sequencing pipetting assembly, including a pipetting module and a cleaning assembly. The pipetting module includes a reagent compartment for storing reagents, a reagent needle assembly for drawing or injecting reagents, and a pipetting drive assembly for driving the movement of the reagent needle assembly. The reagent needle assembly includes a reagent needle and a pipetting pump connected to the reagent needle. The reagent needle draws reagents from the reagent compartment and injects the reagents into a flow cell assembly under the action of the pipetting pump. The cleaning assembly includes a cleaning tank for storing cleaning solution, an inner wall cleaning unit for cleaning the inner wall of the reagent needle, and an outer wall cleaning unit for cleaning the outer wall of the reagent needle. This invention also proposes a gene sequencing system and a gene sequencing pipetting method. The gene sequencing pipetting assembly, pipetting method, and gene sequencing system of this invention utilize a reagent needle to transfer all reagents, which not only effectively simplifies the structure but also facilitates cleaning.