879 results about "Gene sequence" patented technology

The application provides a novel DNA methylation marker TAGMe-3 for tumor identification and use thereof. The TAGMe-3 gene sequence region has significant methylation difference between cancer tissues and paracancer tissues. As long as abnormal high methylation state of the TAGMe-3 gene sequence region is detected, the subject is determined to belong to a tumor high-risk population. Moreover, the significant difference of the TAGMe-3 presented between tumor tissues and non-tumor tissues exists in different types of tumors (Pan-cancer) in a wide range.

The present application relates to a virus of reverse genetic operation construction of NADC34-like PRRSV2. Specifically, the full gene sequence of NADC34-like PRRSV2 BJ1805-2 isolated strain is segmented and connected to the vector by using PCR amplification, enzyme digestion and other methods with pACY177 plasmid as the carrier, and the BJ1805-2 full-length infectious clone recombinant plasmid (rBJ1805-2) is obtained. The infectious clone virus rBJ1805-2 is rescued in vitro, and the first NADC34-like PRRSV2 strain infectious clone platform in China is successfully built. The present application also relates to a modification method for constructing NADC34-like PRRSV2 strain which can adapt to Marc-145 cell passage culture. The infectious clone virus constructed by the present application has good in vitro and in vivo proliferation efficiency, and can cause specific viremia after inoculation in pigs; it has good safety, does not cause fever after inoculation in pigs, and will not cause piglet death. The modified strain can be used as a candidate strain for developing the first NADC34-like PRRSV2 specific vaccine in China, and is conducive to the prevention and control of PRRSV epidemic in China.

The invention discloses L-arabinose isomerase, engineering bacteria and application, the amino acid sequence of the L-arabinose isomerase is shown as SEQ ID NO: 3, and the gene sequence for coding the L-arabinose isomerase is shown as SEQ ID NO: 2. On the basis of a genome of a Peribacillussp.S4 strain, a gene for coding the L-arabinose isomerase is excavated, and a stable prokaryotic expression system is constructed by performing codon optimization on the gene, so that the large-scale controllable production of the L-arabinose isomerase is realized, the yield of the enzyme is remarkably increased, the production cost of the enzyme is reduced, and the method is suitable for industrial production. A stable and economical enzyme source is provided for the production of D-tagatose; the L-arabinose isomerase expressed by the constructed engineering bacteria can significantly improve the yield of D-tagatose, and industrialization of D-tagatose is facilitated.

The application discloses an NLR plug-in gene for improving plant disease resistance and application thereof, and belongs to the field of plant molecular biology and biotechnology.The NLR plug-in gene StEM1 has a whole gene sequence as shown in SEQ ID NO.1 and a CDS sequence as shown in SEQ ID NO.2, and the amino acid sequence of the encoded protein is as shown in SEQ ID NO.3.The gene plays a key role in improving plant disease resistance, especially resistance to field late blight variant bacteria.In the NLR background, overexpression of the gene significantly promotes the disease resistance of tobacco and potato to pathogenic pythium, and the gene is a gene for enhancing plant disease resistance by improving ETI immunity.Overexpression of the gene in the NLR background is obtained by a genetic transformation method, and a strain line is obtained to resist field variant strains, so as to cope with field diseases of crops.The application provides an excellent gene resource for green prevention and control of late blight and disease-resistant genetic engineering breeding.

The invention belongs to the technical field of essential gene prediction, and particularly relates to an essential gene prediction method based on DNA large model and time-frequency domain deep learning fusion, and the method comprises the steps: taking a domain DNA large model as a core representation layer, and obtaining special gene representation through cross-species corpus pre-training and task fine tuning; a T-Block and F-Block dual-channel time-frequency fusion structure is adopted, and the local dependence and long-range regulation relation of a gene sequence is synchronously captured by expanding DFT (Discrete Fourier Transform), complex value attention and iDFT (Initial Discrete Fourier Transform) conversion; designing an efficient modeling reasoning scheme of sliding window slices and gene-level aggregation aiming at an ultra-long sequence; in combination with class imbalance and a noise robust training strategy, cross-cell line / cross-platform transferable threshold output is realized through temperature scaling calibration, an uncertainty quantization and structured interface is matched, and drug target screening and experimental design decision are supported. The system supports the realization of multiple programming languages, and can complete low-delay end-to-end reasoning in a conventional hardware environment.

The invention provides a structure, a sequence and a construction method of a plant virus gene editing vector and application of the plant virus gene editing vector in plant gene editing. The method is characterized in that a TRV2 genome of a tobacco embrittlement virus (TRV) is used for carrying and expressing a small Cas gene AsCas12f and gRNA of the small Cas gene AsCas12f. Preferably, the AsCas12f and the gRNA of the AsCas12f are subjected to tandem expression by utilizing the TRV2 to form an expression cassette structure of the AsCas12f-tRNA-gRNA, the expression cassette substitutes a part of 2b gene sequence of the TRV2 on a TRV2 genome to form a plant gene editing vector pTRV2-Cas12f-gRNA based on the TRV virus, and long-time stable expression and gene editing can be performed after wild type nicotiana benthamiana is inoculated. The invention also provides a method for carrying out gene editing in a wild type plant by utilizing the plant virus.

The invention provides a full-length gene sequence modeling method and system based on a neural network, and the method comprises the steps: constructing a first expression matrix for initial single-cell RNA sequencing data, and carrying out the quality control transformation of the first expression matrix to obtain a second expression matrix; inputting the second expression matrix into a preset binning embedding module to obtain a binning embedding matrix; maintaining and loading a gene pathway set through a knowledge base and a mapping module to obtain a binary mask matrix, and performing mask processing on the binning embedded matrix based on the binary mask matrix to obtain a pathway mask matrix; the path mask matrix is input into a preset attention state space model, the attention state space model comprises an encoder module, a jump connection module and a decoder module which are arranged in sequence, and a reconstruction tensor is output through the decoder module. According to the scheme, an efficient and extensible whole-gene annotation method is provided, and whole-gene expression input can be processed while the calculation efficiency is kept.

The invention discloses a gene sequencing sample data matching method based on a micro-fluidic chip, relates to the technical field of gene sequencing sample matching, and aims to solve the problem that the matching rate is reduced due to inaccurate analysis of gene sequencing samples. According to the method, multi-strategy comparison and candidate set screening are adopted, multi-dimensional comparison of sequences, variation and functions is combined, the accuracy is improved, the adaptability to complex samples is enhanced through quantitative index and biological verification evaluation and dynamic optimization of comparison strategies, the matching reliability and practical value are remarkably improved, the clinical and scientific research diversified requirements are met, and the method is worthy of popularization and application. Sample types such as blood and cells are adapted through a differential lysis strategy, impurities such as proteins and salts are removed through stepped purification, and the nucleic acid concentration and the fragment state are unified in combination with standardized treatment, so that interference is reduced from the source.

The present disclosure, in some aspects, relates to antisense oligonucleotides (ASO) for use in the prevention or treatment of a disease or a condition associated with low- density lipoprotein (LDL) in a subject. In some embodiments, the ASO effects site-directed adenosine-to-inosine (A-to-l) editing of a target adenosine in a target RNA sequence derived from a sequence of an endogenous low-density lipoprotein receptor (LDLR) gene such that: a) the modified LDLR protein has: (i) reduced binding to the inducible degrader of the LDLR protein (IDOL); (ii) increased stability; (iii) improved resistance to IDOL-mediated degradation; (iv) increased LDLR protein expression; and / or (v) increased activity or function to take up LDL; and / or b) editing of the 3'-untranslated region (UTR) of the target RNA leads to an increase in LDLR protein expression and / or stability.

The invention belongs to the technical field of gene editing, and particularly relates to optimized omega RNA and gene editing application thereof. According to the optimized omega RNA, base mutation is carried out on the basis of wild type omega RNA with a gene sequence shown as SEQ ID NO.1, and by taking the 5'end of the wild type omega RNA, the 36th paired base UA is replaced with GC, the 37th paired base UA is replaced with AU, the 38th paired base UG is replaced with AU, the 50th paired base UG is replaced with CG, and the 51st paired base UA is replaced with GC. Compared with wild type omega RNA, the omega RNA has the advantages that the maximum enhancement multiple of the insertion and deletion activity is 16.7 times, 15 pairs of paired bases from the 40th site to the 54th site of the omega RNA are further deleted, and the editing efficiency is further improved.

The invention relates to the technical field of gene engineering, in particular to an L-rhamnose isomerase mutant, a recombinant plasmid, a mutant strain and application. The amino acid sequence of the mutant is as shown in SEQ ID NO.4, and the nucleotide sequence of the coding gene is as shown in SEQ ID NO.3. A specific mutant gene sequence is designed, a recombinant plasmid pET28a (+)-L-Rhi is successfully constructed, the gene sequence is connected between enzyme cutting sites BamH I and Hind III of an escherichia coli expression vector pET-28a (+) to construct a mutant expression vector (the recombinant plasmid pET-28a (+)-L-Rhi), the mutant expression vector is transformed into an escherichia coli BL21 competent cell, a mutant strain is obtained, and the recombinant plasmid pET-28a (+)-L-Rhi is obtained. The obtained mutant strain can overexpress L-rhamnose isomerase, the conversion rate of D-allose is remarkably improved, the thermal stability of the enzyme is remarkably improved through the design of mutation sites, and the half-life period is effectively prolonged.

The invention provides a valine production strain and a construction method and application thereof, a designed acetohydroxy acid synthase mutant is that the 88th basic group of an ilvB gene is changed from a to c, the 382nd basic group is changed from a to g, the 413th basic group is changed from c to t, the gene sequence of a designed artificial operon comprises a promoter, an ilvB (A138V) gene or ilvB (Q30K, S128G, A138V) gene of coding mutated acetohydroxy acid synthase, and an ilvN (G20D, I21D, I21D, I21D, I21D, I21D, I21D, I21D) gene. I22F) gene, a pyk gene for coding pyruvate kinase, and a terminator; by designing a specific acetohydroxyacid synthase mutant and related biological materials and artificial operon, the strain constructed by directional modification of the strain by using a pK18mobsacB system gene editing technology based on allele exchange has the advantages of good genetic stability, high fermentation yield and the like, and valine can be stably produced.

The invention provides an engineered ketoreductase mutant, which comprises a polypeptide sequence, a gene sequence, a recombinant expression vector containing the gene, an engineering strain, a protein expression method of the engineering strain, and a reaction process for selectively synthesizing (R)-5, 7-difluorochroman-4-ol by using the engineered ketoreductase mutant.

The invention discloses a crop ralstonia solanacearum disease prediction method based on an integrated learning model, and the method comprises the following steps: S1, collecting a published 16s rRNA gene sequence related to solanaceae crop bacterial wilt, and carrying out the preprocessing of original sequencing data based on an EasyAmplicon standardized process; s2, performing data dimension reduction by using a principal component analysis algorithm, and retaining 95% of variance; s3, performing hyper-parameter search based on 5-fold cross validation and grid search on the Light GBM model, the CatBoost model and the XGBoost model respectively, and selecting three groups of optimal hyper-parameters of each model; s4, constructing a model according to three groups of optimal hyper-parameters of each selected model, performing prediction, analyzing model result difference based on a Pearson correlation coefficient, and retaining Pearson correlation coefficient mean < lt > with other eight model prediction values; a model of 0.8; and S5, inputting the screened model prediction result into a second-layer element learner RF for integrated learning to obtain a final prediction result.

The invention relates to a recombinant expression cat interferon-omega gene and a preparation method and application thereof, the nucleotide sequence of the cat interferon-omega gene is as shown in SEQ ID NO.1, and the cat interferon-omega gene is obtained by introducing an Fc fusion fragment to the C terminal and / or N terminal of the natural gene sequence of the cat interferon-omega. XTEN or PAS is introduced to prolong a peptide fragment, and original glycosylation modification sites on a natural sequence are reserved. The method comprises the following steps: cloning a cat interferon-omega gene into an expression vector containing double promoters, further transfecting into a cell, carrying out stable cloning and screening, establishing a high-expression cell strain, and carrying out fermentation culture in a bioreactor. According to the invention, the cat interferon-omega gene is subjected to multiple modification and is efficiently expressed in a CHO-K1GS system, so that the protein yield, the stability and the half-life period are remarkably improved; the obtained fusion protein is high in purity and strong in activity, can obviously enhance immune response and protection effect when being matched with cat vaccines, and is good in safety.

The present invention provides a quality control analysis method for third-generation sequencing, which includes performing raw sequencing on the extracted gene sequence to obtain a POD5 text of the initial data. The POD5 text is then converted into a FASTQ file, and adapter data is removed. Quality assessment is then performed to obtain read data. The read data is then aligned and analyzed with the target base sequence of theoretical length, impurities are filtered, and finally, visual detection results are obtained. The present invention also provides a gene extraction method, which forms a highly optimized and reliable bioinformatics analysis process from raw data acquisition, preprocessing, quality assessment, alignment analysis, to final report generation.

The invention discloses an SNP molecular marker for increasing the milk yield of Holstein cattle and application of the SNP molecular marker, the SNP molecular marker is located at a mutation site of a Holstein cattle genome Leptin gene, the gene number of the Leptin gene in an NCBI database is NC037333.1, the SNP molecular marker is located at the 1761th basic group of a cDNA sequence of the Leptin gene, and the mutation basic group is A or G. When the basic group of the SNP molecular marker is A, the genotype is AA or AG, and the milk fat rate and the milk protein rate of Holstein cow milk production are high; when the basic group of the SNP molecular marker is G, the genotype is GG, the milk yield of Holstein cattle in 305 days is high, and the milk quality is not obviously reduced. The marker is applied to genetic improvement of dairy cows, and the frequency of dominant alleles can be improved generation by generation by selecting individuals carrying the dominant alleles, so that the lactation performance of the dairy cows is effectively improved, the breeding efficiency is optimized, and the competitive advantage in the field of dairy cow breeding is enhanced.

The invention discloses an allergen specific IgE and IgG antibody composite quality control product or calibration product as well as a preparation method and application thereof, and belongs to the technical field of biological detection. The preparation method comprises the following steps: obtaining a whole-genome sequence comprising a human IgE constant region gene sequence, a human IgG4 constant region gene sequence and a human IgG1 constant region gene sequence, and preparing a fusion protein simultaneously containing a human IgE Fc segment and a human IgG Fc segment; immunizing a healthy animal by using the allergen to obtain a purified antibody; and carrying out chemical coupling on the purified antibody and the fusion protein to obtain allergen specific IgE and IgG antibodies. By constructing allergen specific IgE and IgG antibodies, the kit can be simultaneously applied to detection of allergens IgE and IgG, has the advantages of high titer, high purity, good dilution linearity and the like, and is beneficial to improvement of diagnosis accuracy and treatment effectiveness.

The invention provides a multi-stream fusion gene sequence prediction method based on Mama and a double-coding strategy, and aims to improve semantic understanding and modeling capability of DNA sequences, so as to improve accuracy and generalization performance of protein expression value prediction. The method comprises the following steps: synchronously processing a forward chain and a reverse complementary chain of a DNA sequence: carrying out word segmentation and vectorization on the reverse complementary chain by adopting BPE coding to capture a semantic fragment; and the forward chain is subjected to one-hot coding and is linearly mapped into dense representation. Deep features of the two features are extracted through Mama Block and then are spliced and fused, then LSTM is input to model context dependence, and finally an expression value is output through a full connection layer. According to the method, the problems that biological semantic fragments are difficult to capture by One-Hot coding and functional domains are likely to be split by BPE coding are effectively solved. On the basis of a Mama framework, a local structure and global semantics are effectively combined, and the method is good at capturing long-distance dependence while low calculation complexity is kept, and is suitable for tasks such as gene design and protein expression regulation and control.

The invention provides application of Ustilaginoidea virens secretory protein UvWMT and a coding gene thereof in prevention and control of plant diseases. The gene sequence of the Ustilaginoidea virens secretory protein UvWMT is researched, found and cloned for the first time, and the new application of the Ustilaginoidea virens secretory protein UvWMT in disease-resistant breeding of crops such as rice is developed. Research finds that the protein is a key pathogenic factor in the process that rice is infected by ustilaginoidea virens, heterologous expression experiments further find that heterologous expression of UvWMT in rice can significantly improve the resistance of crops to various pathogenic bacteria such as ustilaginoidea virens, magnaporthe oryzae and xanthomonas oryzae, and has no significant difference in key agronomic traits compared with a wild type. An important theoretical basis is provided for rice disease-resistant breeding, crops can be safely and effectively endowed with broad-spectrum disease resistance by heterologous expression of the gene, and the gene has great application prospects and economic values in the aspects of crop breeding disease resistance improvement, rice disease prevention and control and crop yield increase and pesticide reduction.

The invention provides a pathogenic driver gene prediction method based on biological network fusion and related equipment. The method comprises the following steps: acquiring data of various driver genes for training; constructing an initial gene relationship map based on protein interaction, gene sequence similarity, KEGG pathway co-occurrence, a gene co-expression mode and semantic similarity of a gene ontology, and embedding various human driven gene data for training into each node in the initial gene relationship map to obtain various gene relationship maps; performing dynamic adjustment on each gene relationship map through edge discarding, feature discarding and difficult sample recognition enhancement to obtain an adjusted gene relationship map for training the constructed pathogenic driving gene prediction model to obtain a trained pathogenic driving gene prediction model; inputting the target driver gene data into the trained pathogenic driver gene prediction model for prediction to obtain a prediction result; and the accuracy and robustness of pathogenic driver gene prediction are improved.

The invention relates to the technical field of biotechnology and plant genetic engineering, in particular to a rice single-base editor and application thereof. The gene sequence of the rice single-base editor at least comprises: (1) a nucleotide sequence as shown in SEQ ID NO.1; (2) a nucleotide sequence which replaces one or more nucleotide sequences in the nucleotide sequence as shown in SEQ ID NO.1 and can be used for rice genome shearing; (3) a nucleotide sequence which is obtained by adding one or more nucleotide sequences into the nucleotide sequence as shown in SEQ ID NO.1 and can be used for rice genome shearing; and (4) a nucleotide sequence which is obtained by deleting one or more nucleotide sequences in the nucleotide sequence as shown in SEQ ID NO.1 and can be used for rice genome shearing. Compared with a traditional single-base editor, the rice single-base editor provided by the invention has the advantage that the editing efficiency is improved when single-base replacement of rice specific gene loci is carried out.

The invention discloses an efficient gene editing system for streptomyces as well as a construction method and application of the efficient gene editing system. The system comprises a TnpB nuclease which can be expressed in streptomyces and a guide RNA, wherein the guide RNA comprises an RNA skeleton, a gene targeting section and a gene sequence of hepatitis delta virus (HDV) ribozyme. Wherein the gene targeting segment is located at the 3'end of the RNA skeleton and is a nucleic acid fragment with the length of 12-40bp after a TAM sequence (5 'TTGAT) on a target gene; the hepatitis D virus (HDV) ribozyme is used for stabilizing the RNA skeleton-gene targeting section structure. According to the streptomyces mini-gene editing tool provided by the invention, in the presence of a homologous repair template, the gene editing efficiency can reach 70-100% in streptomyces, and the gene editing efficiency under the action of the guide RNA after rational design can reach 100%.

The invention belongs to the technical field of microorganisms, and discloses saline-alkaline tolerant halomonas and application thereof. The Franzmannia qiaohouensis is separated from rhizosphere soil of broom corn millet in saline-alkali soil, the strain number is STM-122, the preservation number is CGMCC No.36349, the classification status is clear through 16S rRNA (ribosomal ribonucleic acid) gene sequence identification and phylogenetic analysis, and the Franzmannia qiaohouensis has the functions of dissolving phosphorus and producing EPS (expandable polystyrene), auxin and siderophore. Through bacterial suspension seed soaking treatment, the germination potential and germination rate of broom corn millet seeds under saline-alkali stress can be remarkably improved, and the indexes of seedling root length, plant height and the like are promoted to be improved; meanwhile, the invention provides a complete preparation method of the bacterial strain fermented bacterial fertilizer, and the viable count of the obtained fermented bacterial fertilizer is greater than or equal to 1 * 10 < 8 > CFU / g. The method can enrich salt-tolerant microbial resources, and provides technical support for planting broom corn millet and other crops in saline-alkali soil.

The invention discloses a primer for diagnosis of early pregnancy of sows. The nucleotide sequence of the primer is shown as follows: SRY-O-F: 5 '-CAGCAAAATATTCTCGCCTTGG-3', and the nucleotide sequence of the primer is shown as follows. And SRY-O-R: 5 '-CATCCTCTCTCTACGC-3', and SRY-O-R: 5 '- SRY-I-F: 5 '-ATATTCTCGCCTTGGGG-3', SRY-I-F: 5 '- And SRY-I-R is 5 '-GCTTTCGGCTTCTGTA-3', and the formula is shown in the description. Two pairs of primers with high sensitivity and strong specificity are designed through comparative analysis of pig SRY gene sequences, fetal free DNA in peripheral blood of sows is used as a template, and the specificity and sensitivity of PCR are enhanced through amplification of the two pairs of primers. Through two rounds of PCR amplification, whether the sow is pregnant or not can be detected on the 18th day of pregnancy, and the accuracy of the detection result is extremely high and reaches 100%. Compared with the prior art that the ZFY gene is used for detecting the sows on the twentieth breeding day, the method has the advantages that the detection time can be advanced to the eighteenth breeding day, and the detection accuracy is greatly improved (100% vs 10%). According to the method, powerful technical support is provided for early and accurate identification of the non-pregnant sows, reduction of non-production days and feeding cost and smooth execution of a production plan.

The invention discloses a virus gene sequence host prediction method and system, and belongs to the technical field of biological information analysis, and the method comprises the steps: dividing a virus gene sequence into ordered k-mer word sequences, and carrying out the vectorization of the k-mer word sequences through a pre-trained BERT language model, and obtaining an embedded vector; mapping the embedded vector of the high-dimensional space into a low-dimensional space with a fixed dimension by adopting an average pooling method, and reducing the embedded dimension to obtain a feature vector representing an original gene sequence; the feature vectors are input into a pre-trained classification network model to predict the likelihood that the sequence is infected with a particular host. According to the method, the complete virus gene sequence is directly used as input, so that information loss possibly caused by dependence on statistical characteristics is avoided, and the accuracy of characteristic extraction is remarkably improved.

The invention belongs to the technical field of biomedical engineering, and particularly relates to a polypeptide preparation method for preparing a GLP-1 analogue through tandem expression. According to the method, a coding gene sequence of the GLP-1 analogue is repeatedly connected in series for a plurality of times, and then expression preparation is carried out on the basis of a genetic engineering technology. The inventor optimizes a series-connected polypeptide structure by adding a section of key peptide sequence. The key peptide serving as a leading peptide can greatly improve the expression quantity of the strain in the fermentation expression process; as a linked peptide, the key peptide sequence can effectively improve the recognition capability of enzyme digestion sites on one hand and can adjust the overall isoelectric point of the sequence on the other hand, so that the solubility, enzyme digestion yield and purity of a target product can be improved. Preliminary experiment results show that the expression quantity of the polypeptide prepared through expression is extremely high, the enzyme digestion efficiency is good, the good enzyme digestion efficiency can be achieved under the condition that the enzyme dosage is low, and the yield and purity of the final GLP-1 analogue are high.

The present application relates to the technical field of genetic engineering, and in particular to a high-yield sophorolipid gene knockout engineering bacterium, a construction method thereof and an application, the engineering bacterium taking Bombus bornensis Candida as a starting strain, and using a gF-Hph-gR nucleotide sequence to replace a gene sequence seq2 in a sophorolipid synthesis path for regulating a lactonization reaction ， The gF-Hph-gR nucleotide sequence is SEQ ID No. 1, and the seq2 gene sequence is SEQ ID No. 2. The method provided by the present application uses fusion PCR to construct a seq2 gene knockout box with a resistance screening marker, and the shortest time required is 1 day, which provides a new way for efficiently constructing a sophorolipid production engineering strain. The high-yield sophorolipid gene knockout strain provided by the present application lays a foundation for large-scale industrial production of sophorolipid and meets market demand, and has a wide application prospect, economic value and social benefit.

The invention provides a rapid processing method for HPV (human papillomavirus) gene sequence data. The method relates to the technical field of data processing, and comprises the following steps: acquiring and integrating HPV sequence data, host transcriptome data and clinical association data to form a comprehensive input matrix; performing feature screening on the comprehensive input matrix by adopting a bee colony optimization algorithm based on reinforcement learning to obtain an optimal feature subset; according to the bee colony optimization algorithm, global search of the bee colony algorithm is combined with a dynamic decision-making mechanism of reinforcement learning, and a feature selection strategy is dynamically adjusted; and constructing an HPV subtype accurate typing and variation detection model based on multi-task deep learning, performing deep coding and weighted fusion on the optimal feature subset through an unstable attention mechanism, and outputting two key prediction results of subtype probability and mutation site probability. According to the method, the accuracy and biological interpretability of virus typing and variation detection are improved, and efficient extraction and multi-task accurate recognition of high-dimensional features are realized.

The invention discloses a self-assembled ferritin nano antigen particle and application thereof in preparation of a duck hepatitis A vaccine. The VP1 part in the duck hepatitis A virus P1 protein is connected with the N end of a self-assembled ferritin nanoparticle subunit through a connecting sequence to obtain the fusion protein, and the amino acid sequence of the fusion protein is shown as SEQ ID NO.1; in order to improve the expression quantity or expression efficiency of the fusion protein in bombyx mori, a nucleotide sequence of a coding gene of the fusion protein is optimized and modified according to the codon preference of bombyx mori, and an optimized gene sequence with the nucleotide sequence shown as SEQ ID NO.2 is obtained; the fusion protein is subjected to single-site or multi-site mutation, the mutant with obviously improved titer is obtained through screening, and the soluble expression quantity of the mutant is obviously improved. According to the invention, a silkworm or insect cell eukaryotic expression system is used for expressing recombinant protein, and an expression product causes a neutralizing anti-duck hepatitis A virus antibody.