2 results about "Biochemical function" patented technology

This invention relates to the field of surface engineering technology, and discloses a temperature-sensitive and wear-resistant dual-phase high-entropy ceramic particle coating and its preparation method. The coating consists of transition metal main components, osteogenic active elements, broad-spectrum antibacterial elements, carbon elements, and nitrogen elements, forming a dual-phase high-entropy solid solution containing a carbide main phase and a nitride secondary phase. During preparation, the powders are mixed and ball-milled, then deposited under vacuum plasma spraying. The chamber pressure is adjusted to suppress the volatilization of biochemical elements, allowing them to dissolve in the crystal lattice. This coating utilizes the stress field of the dual-phase structure to anchor the biochemical functional elements, suppressing temperature-sensitive stress cracking and element loss, achieving structural stability and long-term synergistic effects of osteogenic and antibacterial properties.

This invention provides an application of the camptothecin oxygen methyltransferase CaOMT3 in catalyzing the oxygen methylation modification of hydroxycamptothecin and quercetin. Through transcriptomic and genomic analysis, four oxygen methyltransferases (CaOMT3) were identified. BECAUSE OF Genes were found to be clustered together; further analysis of metabolite and transcriptome co-expression revealed a gene significantly positively correlated with rhamnine accumulation. BECAUSE OF Genes, that is CaOMT3 Biochemical functional verification showed that CaOMT1, CaOMT2, and CaOMT3 can all catalyze the reaction of 9- and 10-hydroxycamptothecin. O Methylation yields the corresponding methoxycamptothecin. When quercetin is used as a substrate, CaOMT1 and CaOMT3 exhibit bifunctional catalytic activity, capable of simultaneously catalyzing the methylation of the 4'- and 7'-hydroxyl sites. O α-methylation yields rhamnosin, tamarindin, and phytoflavin; while CaOMT2 only catalyzes the 4'-hydroxyl site. Enzymatic kinetic analysis showed that CaOMT3's catalytic efficiency for quercetin ( k cat / K (m) is the highest. This invention provides a new enzyme tool and technological foundation for the synthetic biology research and industrial fermentation production of rhamnine and methoxycamptothecin.