12 results about "Biochemical function" patented technology

The present invention relates to methods and products for assessing autophagic flux, and more specifically provides a biochemical functional measure used to quantify autophagic flux in sub-populations of blood cells. The present invention also provides lysosomal system markers for use in the methods described herein, systems for assessing autophagic flux, methods of identifying markers indicative of autophagic flux, and methods of treating subjects found to be suffering from, or susceptible to, a disease, condition or state associated with autophagic flux dysfunction using the methods described herein.

The invention discloses a polypeptide compound and application thereof in preparation of a product for inhibiting ATP enzyme hydrolysis activity. The invention provides a polypeptide which is any one of the following: A1, the amino acid sequence of the polypeptide is sequence 1; a2, tag protein is added to the tail end of the polypeptide shown in A1, and the fused polypeptide is obtained. According to the method, multi-round progressive sequence optimization is performed by focusing a prototype peptide targeting 2C, simulating comprehensive utilization of a virtual neural network training model and actually-measured biochemical function evaluation in multiple aspects such as enzymatic inhibition and binding force evaluation, and a series of candidate peptides with relatively high comprehensive evaluation and shortened sequences are obtained; and a structural basis is provided for the design of a new round of antiviral peptidomimetic small molecules. Finally, candidate peptides are obtained through the research, and theoretical basis and technical support are provided for development of ATP enzyme inhibitors.

The application discloses a sewage treatment method, system and equipment based on AOA transformation, relates to the related field of sewage treatment, and comprises the following steps: according to the pollution sensitive attribute, a distributed deployment of a multi-modal sensor is carried out; a control center receives three-dimensional pollutant migration dynamic data returned by a biochemical function sensor array group, and constructs a three-dimensional space-time graph; a pollution factor degradation kinetics path fitting is carried out to obtain a carbon-nitrogen-phosphorus synergistic degradation model; the model is projected to a biochemical reaction area group, short-period pollutant degradation efficiency tracking is carried out after edge fraction partition isolation parameter adjustment compensation is performed, and a three-dimensional process efficiency thermal diagram is obtained; multi-objective optimization instruction sets are output through pool linkage regulation and control optimization; long-period sewage treatment is carried out until the pollutant concentration distribution evolution exceeds the preset threshold boundary, and AOA collaborative parameter adjustment updating is triggered. The technical problems of insufficient precision and adaptability of the existing sewage treatment are solved, and the technical effect of self-adaptive and precise optimization control of the AOA process is achieved.

The invention discloses a sewage treatment method, system and equipment based on AOA transformation, and relates to the related field of sewage treatment.The sewage treatment method comprises the steps that according to pollution sensitive attributes, distributed deployment of multi-modal sensors is conducted; the control middle station receives the three-dimensional pollutant migration dynamic data returned by the biochemical function sensing array group and constructs a three-dimensional space-time atlas; carrying out pollution factor degradation dynamic path fitting to obtain a carbon-nitrogen-phosphorus cooperative digestion model; projecting to a biochemical reaction zone group, executing edge-level partition isolation parameter adjustment compensation, and then carrying out short-period pollutant digestion efficiency tracking to obtain a three-dimensional process efficiency thermodynamic diagram; pool-divided linkage regulation and control optimization is carried out to output a multi-target optimization instruction set; and executing long-period sewage treatment until the pollutant concentration distribution evolution exceeds a preset threshold boundary, and triggering AOA coordinated parameter adjustment updating. The technical problem that existing sewage treatment is insufficient in accuracy and adaptability is solved, and the technical effect of self-adaptive accurate optimization control over the AOA process is achieved.

The application provides a self-shrinking hydrogel as well as a preparation method and application thereof, wherein the modified chitosan derivative has a structure of formula I. After the chitosan is modified in the application, the solubility of the chitosan is effectively improved, so that the chitosan can be uniformly dispersed in PBS or ultrapure water, the application prospect is widened, and the modified chitosan derivative is more suitable for accelerating wound repair in biochemical functions. The self-shrinking hydrogel containing the modified chitosan derivative is sensitive to temperature in the application, can produce deformation and shrinkage with the increase of temperature, can generate centripetal force on the edge tissue of a wound when applied to the wound, and is beneficial to wound healing.

This invention relates to the field of surface engineering technology, and discloses a temperature-sensitive and wear-resistant dual-phase high-entropy ceramic particle coating and its preparation method. The coating consists of transition metal main components, osteogenic active elements, broad-spectrum antibacterial elements, carbon elements, and nitrogen elements, forming a dual-phase high-entropy solid solution containing a carbide main phase and a nitride secondary phase. During preparation, the powders are mixed and ball-milled, then deposited under vacuum plasma spraying. The chamber pressure is adjusted to suppress the volatilization of biochemical elements, allowing them to dissolve in the crystal lattice. This coating utilizes the stress field of the dual-phase structure to anchor the biochemical functional elements, suppressing temperature-sensitive stress cracking and element loss, achieving structural stability and long-term synergistic effects of osteogenic and antibacterial properties.

The invention discloses an ATM gene knockout THP-1 cell line as well as a construction method and application thereof, and belongs to the technical field of biology. The gRNA for targeted knockout of the ATM gene is provided, the ATM gene of the THP-1 cell line is knocked out in combination with a CRISPR / Cas9 gene editing technology, and the THP-1 cell line with the knocked-out ATM gene is successfully obtained. The cell line stably lacks an ATM gene, can be normally differentiated into macrophages, and can be further polarized into M1 and M2 macrophages. The THP-1 cell line with the ATM gene knocked out, which is constructed by the invention, can be widely applied to research on in-vitro and macrophage physiological and biochemical functions of the ATM gene and immune response drug targets.

The present invention provides a microneedle moxibustion device comprising a microneedle layer, a skin-attaching layer and a moxibustion material layer, wherein the microneedle layer and the moxibustion material layer are respectively located on two sides of the skin-attaching layer that are arranged opposite to each other, a plurality of microneedles are arranged on the side of the microneedle layer away from the skin-attaching layer, and the moxibustion material layer comprises a combustible material; with the above structure, when the skin-attaching layer is attached to human skin, the microneedles on the microneedle layer penetrate into the human skin layer, causing slight damage to the human skin, without causing skin bleeding, and bringing painlessness to the human body, thereby reducing the patient's pain level; and the moxibustion material layer of another layer of the attachment layer contains a combustible material, and the heat and light produced by burning the combustible material stimulate the human acupuncture points or specific parts, and adjust the disordered physiological and biochemical functions of the human body by stimulating the activity of meridian qi, thereby achieving the purpose of preventing and curing diseases, and the inflammatory effect caused by the microneedle insertion and the stimulating effect of moxibustion are superimposed on each other, effectively filling the defect of indirect moxibustion lacking moxibustion-induced inflammation.

The present invention relates to methods and products for assessing autophagic flux, and more specifically provides a biochemical functional measure used to quantify autophagic flux in sub-populations of blood cells. The present invention also provides lysosomal system markers for use in the methods described herein, systems for assessing autophagic flux, methods of identifying markers indicative of autophagic flux, and methods of treating subjects found to be suffering from, or susceptible to, a disease, condition or state associated with autophagic flux dysfunction using the methods described herein.

The application provides a long-chain non-coding RNA MSTRG.5970.28 and application thereof, a product and a method for goose ovary development, relates to the technical field of biology, and provides a new long-chain non-coding RNA MSTRG.5970.28; the lncRNA MSTRG.5970.28 is related to the proliferation and apoptosis of goose follicular granulosa cells; by regulating the expression of the lncRNA MSTRG.5970.28, the related miRNA and gene of the proliferation and apoptosis of the follicular granulosa cells can be correspondingly regulated. By applying the lncRNA MSTRG.5970.28 and the corresponding product, the research on the proliferation and apoptosis of the follicular granulosa cells and the physiological and biochemical functions can be effectively guaranteed. In addition, by regulating the proliferation and apoptosis of the follicular granulosa cells through the lncRNA MSTRG.5970.28, the population reproductive capacity can be improved while the excellent characteristics of the goose germplasm resources are maintained, so that the industrialization development of geese is promoted, and the economic benefits are improved.

This invention provides an application of the camptothecin oxygen methyltransferase CaOMT3 in catalyzing the oxygen methylation modification of hydroxycamptothecin and quercetin. Through transcriptomic and genomic analysis, four oxygen methyltransferases (CaOMT3) were identified. BECAUSE OF Genes were found to be clustered together; further analysis of metabolite and transcriptome co-expression revealed a gene significantly positively correlated with rhamnine accumulation. BECAUSE OF Genes, that is CaOMT3 Biochemical functional verification showed that CaOMT1, CaOMT2, and CaOMT3 can all catalyze the reaction of 9- and 10-hydroxycamptothecin. O Methylation yields the corresponding methoxycamptothecin. When quercetin is used as a substrate, CaOMT1 and CaOMT3 exhibit bifunctional catalytic activity, capable of simultaneously catalyzing the methylation of the 4'- and 7'-hydroxyl sites. O α-methylation yields rhamnosin, tamarindin, and phytoflavin; while CaOMT2 only catalyzes the 4'-hydroxyl site. Enzymatic kinetic analysis showed that CaOMT3's catalytic efficiency for quercetin ( k cat / K (m) is the highest. This invention provides a new enzyme tool and technological foundation for the synthetic biology research and industrial fermentation production of rhamnine and methoxycamptothecin.

The invention discloses a nutritional therapy-based functional potion for benefiting qi and nourishing blood and a preparation process thereof, and belongs to the technical field of functional foods.The preparation process comprises the following steps: preparing beta-cyclodextrin, ergothioneine and an iron source into an inclusion compound; the inclusion compound, the colloid, the red ginseng, the red date concentrated juice, the lemon concentrated juice and the like are added into a reaction container to form a synergistic effect mechanism of tonifying qi, generating blood and promoting absorption, and the traditional Chinese medicine composition has a more remarkable hematopoietic function improvement effect compared with a single iron agent or a traditional blood replenishing traditional Chinese medicine; the ergothioneine can remarkably promote absorption of iron, and the iron source and the ergothioneine are prepared into the inclusion compound, so that the stability of the ergothioneine and the iron is improved, the damage of gastric acid is avoided, and the bioavailability of the potion is improved; through synergistic compatibility of the active component of red ginseng and iron, the multiplication capacity of bone marrow stroma cells can be remarkably enhanced, and the qi and blood biochemical function of the body is improved; ginsenoside contained in the red ginseng has the effect of promoting hematopoiesis and can activate proliferation and differentiation of hematopoietic stem cells.