5 results about "Biochemical function" patented technology

The invention discloses a polypeptide compound and application thereof in preparation of a product for inhibiting ATP enzyme hydrolysis activity. The invention provides a polypeptide which is any one of the following: A1, the amino acid sequence of the polypeptide is sequence 1; a2, tag protein is added to the tail end of the polypeptide shown in A1, and the fused polypeptide is obtained. According to the method, multi-round progressive sequence optimization is performed by focusing a prototype peptide targeting 2C, simulating comprehensive utilization of a virtual neural network training model and actually-measured biochemical function evaluation in multiple aspects such as enzymatic inhibition and binding force evaluation, and a series of candidate peptides with relatively high comprehensive evaluation and shortened sequences are obtained; and a structural basis is provided for the design of a new round of antiviral peptidomimetic small molecules. Finally, candidate peptides are obtained through the research, and theoretical basis and technical support are provided for development of ATP enzyme inhibitors.

The application provides a self-shrinking hydrogel as well as a preparation method and application thereof, wherein the modified chitosan derivative has a structure of formula I. After the chitosan is modified in the application, the solubility of the chitosan is effectively improved, so that the chitosan can be uniformly dispersed in PBS or ultrapure water, the application prospect is widened, and the modified chitosan derivative is more suitable for accelerating wound repair in biochemical functions. The self-shrinking hydrogel containing the modified chitosan derivative is sensitive to temperature in the application, can produce deformation and shrinkage with the increase of temperature, can generate centripetal force on the edge tissue of a wound when applied to the wound, and is beneficial to wound healing.

This invention relates to the field of surface engineering technology, and discloses a temperature-sensitive and wear-resistant dual-phase high-entropy ceramic particle coating and its preparation method. The coating consists of transition metal main components, osteogenic active elements, broad-spectrum antibacterial elements, carbon elements, and nitrogen elements, forming a dual-phase high-entropy solid solution containing a carbide main phase and a nitride secondary phase. During preparation, the powders are mixed and ball-milled, then deposited under vacuum plasma spraying. The chamber pressure is adjusted to suppress the volatilization of biochemical elements, allowing them to dissolve in the crystal lattice. This coating utilizes the stress field of the dual-phase structure to anchor the biochemical functional elements, suppressing temperature-sensitive stress cracking and element loss, achieving structural stability and long-term synergistic effects of osteogenic and antibacterial properties.

The invention discloses an ATM gene knockout THP-1 cell line as well as a construction method and application thereof, and belongs to the technical field of biology. The gRNA for targeted knockout of the ATM gene is provided, the ATM gene of the THP-1 cell line is knocked out in combination with a CRISPR / Cas9 gene editing technology, and the THP-1 cell line with the knocked-out ATM gene is successfully obtained. The cell line stably lacks an ATM gene, can be normally differentiated into macrophages, and can be further polarized into M1 and M2 macrophages. The THP-1 cell line with the ATM gene knocked out, which is constructed by the invention, can be widely applied to research on in-vitro and macrophage physiological and biochemical functions of the ATM gene and immune response drug targets.

This invention provides an application of the camptothecin oxygen methyltransferase CaOMT3 in catalyzing the oxygen methylation modification of hydroxycamptothecin and quercetin. Through transcriptomic and genomic analysis, four oxygen methyltransferases (CaOMT3) were identified. BECAUSE OF Genes were found to be clustered together; further analysis of metabolite and transcriptome co-expression revealed a gene significantly positively correlated with rhamnine accumulation. BECAUSE OF Genes, that is CaOMT3 Biochemical functional verification showed that CaOMT1, CaOMT2, and CaOMT3 can all catalyze the reaction of 9- and 10-hydroxycamptothecin. O Methylation yields the corresponding methoxycamptothecin. When quercetin is used as a substrate, CaOMT1 and CaOMT3 exhibit bifunctional catalytic activity, capable of simultaneously catalyzing the methylation of the 4'- and 7'-hydroxyl sites. O α-methylation yields rhamnosin, tamarindin, and phytoflavin; while CaOMT2 only catalyzes the 4'-hydroxyl site. Enzymatic kinetic analysis showed that CaOMT3's catalytic efficiency for quercetin ( k cat / K (m) is the highest. This invention provides a new enzyme tool and technological foundation for the synthetic biology research and industrial fermentation production of rhamnine and methoxycamptothecin.