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305 results about "Methyltransferase" patented technology
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Methyltransferases are a large group of enzymes that all methylate their substrates but can be split into several subclasses based on their structural features. The most common class of methyltransferases is class I, all of which contain a Rossman fold for binding S-Adenosyl methionine (SAM). Class II methyltransferases contain a SET domain, which are exemplified by SET domain histone methyltransferases, and class III methyltransferases, which are membrane associated. Methyltransferases can also be grouped as different types utilizing different substrates in methyl transfer reactions. These types include protein methyltransferases, DNA/RNA methyltransferases, natural product methyltransferases, and non-SAM dependent methyltransferases. SAM is the classical methyl donor for methyltrasferases, however, examples of other methyl donors are seen in nature. The general mechanism for methyl transfer is a SN2-like nucleophilic attack where the methionine sulfur serves as the nucleophile that transfers the methyl group to the enzyme substrate. SAM is converted to S-Adenosyl homocysteine (SAH) during this process. The breaking of the SAM-methyl bond and the formation of the substrate-methyl bond happen nearly simultaneously. These enzymatic reactions are found in many pathways and are implicated in genetic diseases, cancer, and metabolic diseases.
Recombinant escherichia coli with high yield of ergothioneine and method for preparing ergothioneine by using recombinant escherichia coli
The invention provides recombinant escherichia coli with high yield of ergothioneine and a method for preparing ergothioneine, the recombinant escherichia coli co-expresses ergothioneine anabolic enzymes Egt1 and Egt2, the methyltransferase structural domain of the Egt1 is derived from trichoderma reesei, mycobacterium smegmatis EgtD or rhizopus nigricans, and the methyltransferase structural domain of the Egt2 is derived from the trichoderma reesei, the mycobacterium smegmatis EgtD or the rhizopus nigricans. The histidine sulfoxide synthase structural domain is derived from trichoderma reesei, schizosaccharomyces pombe or rhizopus nigricans, and the Egt2 is derived from claviceps purpurea, neurospora crassa or aspergillus niger. After the recombinant escherichia coli obtained by screening is cultured for 68 hours in a 5L fermentation tank, the yield of ergothioneine can reach 5g / L or above; wherein after recombinant escherichia coli co-expressing Egt1 enzyme (the structural domain of methyltransferase is derived from mycobacterium smegmatis EgtD, and the structural domain of histidine sulfoxide synthase is derived from trichoderma reesei) and Egt2 enzyme (derived from neurospora crassa) are cultured in a 5L fermentation tank for 68 hours, the yield of ergothioneine can reach 8.7 g / L, and the production intensity reaches 127.9 mg / (L.h).
Owner:THE UNITED BIO-TECH (HENGQIN) CO LTD
Biosynthesis inhibitor of migratory locust aggregation pheromone 4-vinyl anisole and application thereof
The invention provides a biosynthesis inhibitor of migratory locust aggregation pheromone 4-vinyl anisole and application of the biosynthesis inhibitor. The biosynthesis inhibitor of migratory locust aggregation pheromone 4-vinylanisole reduces the activity of 4-vinylphenol methyltransferase, and the 4-vinylphenol methyltransferase contains an amino acid sequence selected from the following sequences: (a) a sequence as shown in any one of SEQ ID No.1 and SEQ ID No.3; and (b) a sequence which is obtained by deleting, inserting and / or substituting one or more amino acid residues in the sequence shown in any one of SEQ ID No.1 and SEQ ID No.3 and has the activity of catalyzing generation of migratory locust aggregation pheromone 4-vinyl anisole. The inhibitor can be applied to biological prevention and control of migratory locusts, can keep locusts in a harmless scattered state instead of killing the locusts, provides a new method for biological prevention and control of migratory locusts, and has a wide application prospect.
Owner:INST OF ZOOLOGY CHINESE ACAD OF SCI +1
Biosynthesis method for de novo synthesis of glabridin and strain
The invention relates to the field of biological engineering and technology, in particular to an enzyme for synthesizing glabridin, a biological synthesis method of glabridin and a bacterial strain. The invention discloses a series of glabridin synthesizing enzymes (phenylalanine ammonialyase, cinnamic acid-4-hydrogenase, tyrosine ammonialyase, p-coumaric acid coenzyme A ligase, chalcone synthase, chalcone reductase, chalcone isomerase, isoflavone synthase, 2-hydroxyisoflavone dehydratase, 4 '-oxymethyltransferase, 4'-hydroxymethyltransferase and 4 '-hydroxymethyltransferase) derived from glycyrrhiza glabra for the first time. ) can be used in the preparation of a compound (e.g., isoflavone 2 '-hydrogenase, isoflavone reductase, Vissisoketoreductase, isoprenyltransferase, pterocarpin reductase, oxidative cyclase, demethylase, cytochrome P450 reductase, and the like). The de novo synthesis of the glabridin in a microbial cell factory is realized for the first time, and the application prospect of the glabridin biological manufacturing industry is greatly promoted.
Owner:TSINGHUA UNIVERSITY
Methyltransferase mutant, recombinant vector, recombinant bacterium and application of methyltransferase mutant in production of pterostilbene
The invention discloses a methyltransferase mutant, a recombinant vector, a recombinant bacterium and application of the methyltransferase mutant in production of pterostilbene, and relates to the technical field of bioengineering. According to the methyltransferase mutant provided by the invention, resveratrol generates pterostilbene under the catalytic action of methyltransferase, and compared with the unmutated methyltransferase, the mutated methyltransferase has the advantages that the affinity of enzyme and a substrate is improved, and the resveratrol is promoted to be converted into pterostilbene. The coding gene of the methyltransferase mutant is transferred into a chassis cell or a chassis strain and is fermented, so that the yield of the pterostilbene can be greatly increased, the efficient synthesis of the plant-derived antioxidant pterostilbene in the microbial chassis cell is realized, and the ever-increasing market demand of the pterostilbene can be met.
Owner:GUANGDONG TECHNION ISRAEL INST OF TECH
TRMT61A and application of TRMT61A inhibitor tetramethylthiuram disulfide in tumor immunotherapy
PendingCN120796478AOrganic active ingredientsMicrobiological testing/measurementTumor therapyMethyltransferase
The invention provides application of TRMT61A and its inhibitor tetramethylthiuram disulfide in tumor immunotherapy, and relates to the technical field of biological medicine, clinical research proves that RNA m1A methyltransferase TRMT61A is highly expressed in tumor tissues and is related to poor prognosis of tumor patients, and can be used as one of tumor therapy targets. Meanwhile, high expression of the TRMT61A is related to poor prognosis of a tumor patient receiving anti-PD-1 immunotherapy, and after anti-PD-1 antibody immunotherapy is carried out on the patient with low TRMT61A expression level in the tumor of the patient, pathological complete relief is easier. Tetramethylthiuram disulfide inhibits TRMT61A, inhibits m1A modification regulation of TRMT61A on PD-L1 mRNA, increases PD-L1 protein expression, and is combined with a PD-1 antibody drug to significantly enhance the immunotherapy effect of the anti-PD-1 immune checkpoint drug in treating malignant tumors.
Owner:THE THIRD AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIVERSITY (GUANGZHOU SEVERE MATERNAL TREATMENT CENTER GUANGZHOU ROUJI HOSPITAL)
Protein with dihydrofolate reductase activity and application thereof
The invention provides a protein with dihydrofolate reductase activity and application thereof. The protein provided by the invention is a mutant of dihydrofolate reductase based on a Streptococcus thermophilus source, and the amino acid sequence of the protein is as shown in SEQ ID NO: 1. The protein provided by the invention can catalyze folic acid to be converted into L-tetrahydrofolic acid. Based on the characteristic, the glucose dehydrogenase and the tetrahydrofolate methyltransferase are combined to realize synthesis of the L-5-methyltetrahydrofolate, so that the problems of low yield and environmental pollution of a chemical method for producing the L-5-methyltetrahydrofolate and complex reaction and high substrate cost of a biological method are solved, the production cost of the L-5-methyltetrahydrofolate is reduced, and the yield of the L-5-methyltetrahydrofolate is improved. The synthesis efficiency and the synthesis quality of the L-5-methyltetrahydrofolic acid are improved, and the method has a relatively good industrialization prospect.
Owner:EFFEPHARM (SHANGHAI) CO LTD
Modified o-methyltransferases useful for production of o-methylated phenolic natural products
Disclosed are modified Type 1 O-methyltransferases (OMTs) and methods of using such modified OMTs. In some forms of the modified OMTs, the amino acid corresponding to Phe337 of Sorghum bicolor stilbene OMT (SbSOMT) is substituted with a polar amino acid. In some forms of the modified OMTs, the amino acid corresponding to Asn323 of Sorghum bicolor caffeic acid OMT (SbCOMT) is substituted with a hydrophobic amino acid. In some forms of the modified OMTs, the amino acid corresponding to Ile144 of SbSOMT is substituted with a polar amino acid. In some forms of the modified OMTs, the amino acid corresponding to Asn128 of SbCOMT is substituted with a hydrophobic amino acid.
Owner:THE UNIVERSITY OF HONG KONG
METTL3 inhibitory compounds
The present invention relates to compounds of formula (I) that function as inhibitors of METTL3 (N6-adenosine-methyltransferase 70 kDa subunit) enzyme activity: X—Y—Z5 (I) wherein X, Y and Z are each as defined herein. The present invention also relates to processes for the preparation of these compounds, to pharmaceutical compositions comprising them, and to their use in the treatment of proliferative disorders, such as cancer, and autoimmune diseases, as well as other diseases or conditions in which METTL3 activity 10 is implicated.
Owner:STORM THERAPEUTICS LIMITED
Amide substituted tricyclic guanidino compounds as PRMT5 inhibitors
PCT designated stageWO2025166260A1Organic active ingredientsOrganic chemistryArginineMethyltransferase
Provided herein are compounds of Formula (I) or a pharmaceutically acceptable salt thereof. The provided compounds are useful Protein arginine N-methyltransferase 5 (PRMT5) inhibitors. Also, disclosed are pharmaceutical compositions comprising such compounds and methods of making such compounds. Additional utilities and advantages are described herein.
Owner:IDEAYA BIOSCIENCES INC
Triheterocyclic guanidino compounds as PRMT5 inhibitors
PCT designated stageWO2025166215A1Organic active ingredientsOrganic chemistryArginineMethyltransferase
Provided herein are compounds of Formula (I) or a pharmaceutically acceptable salt thereof. The provided compounds are useful Protein arginine N-methyltransferase 5 (PRMT5) inhibitors. Also, disclosed are pharmaceutical compositions comprising such compounds and methods of making such compounds. Additional utilities and advantages are described herein.
Owner:IDEAYA BIOSCIENCES INC
Synthesis of 1n-methylpseudouridine and 1n-methylpseudouridine phosphates
A method of synthesising a 1N-alkyl derivative of pseudouridine comprises contacting a substrate with a methyltransferase in the presence of a cofactor to produce the 1N-alkyl derivative of pseudouridine. The substrate has a structure of: Formula (I). The 1N-methyl derivative of pseudouridine has a structure of: Formula (II). R1 is a hydroxyl group, a monophosphate group, a diphosphate group, or a triphosphate group. R2 is H or a hydroxyl group. R3 is a methyl group or an ethyl group. The cofactor is a source of a methyl group or an ethyl group. The methyltransferase is configured to transfer the methyl group or ethyl group from the cofactor to the substrate. Enzymatic synthesis of 1N-methyl derivatives of pseudouridine may be more efficient and produce less waste than chemical syntheses. Also provided are methyltransferases and buffers useful in the method; and polynucleotides, expression vectors, and microorganisms useful for producing the methyltransferases.
Owner:UAB BIOMATTER DESIGNS
Amide substituted triheterocyclic guanidino compounds as PRMT5 inhibitors
PCT designated stageWO2025166257A1Organic active ingredientsOrganic chemistryArginineMethyltransferase
Provided herein are compounds of Formula (I) or a pharmaceutically acceptable salt thereof. The provided compounds are useful Protein arginine N-methyltransferase 5 (PRMT5) inhibitors. Also, disclosed are pharmaceutical compositions comprising such compounds and methods of making such compounds. Additional utilities and advantages are described herein.
Owner:IDEAYA BIOSCIENCES INC
Application of MY-1B in the preparation of drugs for improving ovarian senescence
The present invention discloses the application of MY-1B in the preparation of drugs for improving ovarian senescence, belonging to the technical field of biomedicine, aiming to solve the problem of how to effectively delay ovarian senescence and improve ovarian function. Experiments have proved that during ovarian senescence, the mRNA m<supgt;5< / supgt;C modification level and the expression level of methyltransferase in ovarian granulosa cells increase. Supplementation with MY-1B can improve the condition caused by mRNA m Nsun2 expression.
Owner:INST OF ZOOLOGY CHINESE ACAD OF SCI
Use of snip1 as a target in the preparation or screening of antitumor drugs
The application belongs to the technical field of biotechnology and gene therapy, and particularly relates to application of SNIP1 protein as a target in preparation or screening of an antitumor drug. The application finds that the SNIP1 protein is a non-histone substrate of lysine methyltransferase KMT5A, which promotes breast cancer cell growth, invasion and lung metastasis through KMT5A-mediated K301 monomethylation; therefore, the SNIP1 protein can be used as a target for screening of an antitumor drug, and a drug obtained by screening and inhibiting methylation of the K301 site of the SNIP1 protein can be used for antitumor; and the application provides a polypeptide specifically combined with the K301 site of the SNIP1 protein, the polypeptide is combined with the K301 site of the SNIP1 protein, inhibits methylation of the SNIP1 protein, and finally can inhibit tumor growth, proliferation, invasion and metastasis, and can be used as a new targeted antitumor drug.
Owner:SUZHOU QINGRUI BIOTECHNOLOGY CO LTD
Tricyclic amidino compounds as PRMT5 inhibitors
PCT designated stageWO2025166229A1Organic active ingredientsOrganic chemistryArginineMethyltransferase
Provided herein are compounds of Formula (I) or a pharmaceutically acceptable salt thereof. The provided compounds are useful Protein arginine N-methyltransferase 5 (PRMT5) inhibitors. Also, disclosed are pharmaceutical compositions comprising such compounds and methods of making such compounds. Additional utilities and advantages are described herein.
Owner:IDEAYA BIOSCIENCES INC
Application of caffeic acid-O-methyltransferase in regulating and controlling salt tolerance of rice
The invention discloses an application of caffeic acid-O-methyltransferase in regulation and control of salt tolerance of rice. The overexpressed OsCOMT gene disclosed by the invention can remarkably improve the melatonin content and antioxidant level of rice seedlings, so that the tolerance of plants to salt stress is enhanced, and the plants can maintain normal growth under the condition of salt stress; the deletion of the gene OsCOMT leads to the increase of the sensitivity of seedling-stage rice to salt stress, and exogenous melatonin can recover the salt-sensitive phenotype of a mutant-knocked-out plant and improve the photosynthetic rate of the mutant-knocked-out plant. The OsCOMT gene and the encoding protein thereof have important theoretical and practical significance on research of a plant stress tolerance mechanism and improvement of stress tolerance and related traits of plants, can play an important role in plant stress tolerance gene engineering improvement, and are wide in application prospect.
Owner:YANGZHOU UNIV
Tricyclic guanidino compounds as PRMT5 inhibitors
PCT designated stageWO2025166274A1Organic active ingredientsOrganic chemistryArgininePharmaceutical drug
Provided herein are compounds of Formula (I) or a pharmaceutically acceptable salt thereof. The provided compounds are useful Protein arginine N-methyltransferase 5 (PRMT5) inhibitors. Also, disclosed are pharmaceutical compositions comprising such compounds and methods of making such compounds. Additional utilities and advantages are described herein.
Owner:IDEAYA BIOSCIENCES INC
Application of CsNOMT gene and sakuranetin in prevention and treatment of tea tree anthracnose
The invention discloses application of a CsNOMT gene and sakuranetin in prevention and treatment of tea tree anthracnose. It is found for the first time that methyltransferase encoded by the tea tree-derived CsNOMT gene can efficiently catalyze naringenin to be converted into sakuranetin, the endogenous phytoalexin level of a tea tree is remarkably improved, and then the disease resistance of the tea tree-derived CsNOMT gene to Colletotrichum carolinae is enhanced. The silence of the CsNOMT gene can reduce the sakuranetin content by 30-40%, resulting in the increase of the scab area; and by overexpressing the gene, the sakuranetin content is increased by 30%, and the scab area is remarkably reduced. In addition, the growth of colletotrichum can be effectively inhibited by exogenously spraying 0.05-0.3 mM of sakuranetin, and the optimal concentration is 0.1-0.3 mM. The invention provides a new strategy for green prevention and control of tea tree anthracnose, and has the characteristics of high efficiency, safety and environmental friendliness.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
CuCCoAOMT1 gene of Chimonobambusa auriculata, and protein and application of CuCCoAOMT1 gene
The invention discloses a Chimonobambusa chensinensis CuCCoAOMT1 gene as well as a protein and application thereof, belongs to the technical field of plant genetic engineering, and particularly relates to a Chimonobambusa chensinensis caffeoyl-coenzyme A-O-methyltransferase CuCCoAOMT1 gene, and a nucleotide sequence of the CuCCoAOMT1 gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the CuCCoAOMT1 gene. The invention further discloses application of the CuCCoAOMT1 gene in regulation and control of the drought resistance of gramineous plants. By overexpressing the caffeoyl-coenzyme A-O-methyltransferase CuCCoAOMT1 gene in the rice, the lignin content in the rice can be effectively increased, the thickening of rice cell walls is promoted, the drought resistance of rice plants is improved, and a new gene resource is provided for plant material property improvement gene engineering.
Owner:INT CENT FOR BAMBOO & RATTAN
Serine hydroxymethyltransferase mutant and application thereof in production of L-serine
The invention discloses a serine hydroxymethyltransferase mutant and an application thereof in production of L-serin.The serine hydroxymethyltransferase mutant with the specific enzyme activity remarkably improved is obtained by conducting saturated mutation on serine hydroxymethyltransferase from escherichia coli and conducting screening, and the serine hydroxymethyltransferase mutant is applied to synthesis of L-serine. The serine hydroxymethyltransferase disclosed by the invention can be used for effectively improving the efficiency of catalyzing glycine and formaldehyde to synthesize L-serine by using the serine hydroxymethyltransferase, reducing the enzyme dosage, remarkably reducing the production cost and reducing the separation difficulty of downstream products.
Owner:HENAN ZHONGYUAN YUZE BIOTECHNOLOGY CO LTD
Microorganism for efficiently producing ergothioneine as well as preparation method and application thereof
The invention belongs to the field of industrial application of microbial fermentation, and relates to a microorganism for efficiently producing ergothioneine as well as a preparation method and application of the microorganism. Specifically, the method comprises the following steps: firstly, artificially creating a promoter with a group response property by utilizing saturation mutation and screening of a fluorescence microscope; then, the promoter is used for mediating host bacterium heterologous histidine methyltransferase, sulfoxide synthase and PLP dependent C-S lyase to improve the yield of ergothioneine; on the basis, an endogenous promoter HisJ of host bacteria is replaced, and endogenous cysteine lyase yhaM is knocked out, so that the yield of the ergothioneine is increased again. According to combination of the above strategies, a heuristic and commercialized production thought is provided for efficient synthesis of ergothioneine, and a foundation is laid for reduction of the synthesis cost of ergothioneine.
Owner:BEIJING TECH & BUSINESS UNIV
3 '-O-methyltransferase mutant and application thereof
The invention relates to a 3 '-O-methyltransferase mutant and application thereof. The 3 '-O-methyltransferase mutant is obtained by mutating tryptophan W at the 284th position of wild type plant oxygen methyltransferase derived from rice into alanine A, the amino acid sequence of the wild type plant oxygen methyltransferase is as shown in SEQ ID NO: 1, and the amino acid sequence of the 3'-O-methyltransferase mutant is as shown in SEQ ID NO: 2. According to the application, a key amino acid site of the catalytic activity of the flavonoid 3 '-O-methyltransferase is determined through a semi-rationally designed enzyme engineering strategy, a dominant mutant is obtained, and the dominant effect of oriented modification on the synthesis of methylated flavonoid compounds by catalyzing flavonoid compounds through the flavonoid 3'-O-methyltransferase is defined; the obtained 3 '-O-methyltransferase mutant has excellent catalytic performance, and meanwhile, the yield of methylated flavonoid compounds is further increased by optimizing and strengthening a metabolic pathway.
Owner:SHAANXI UNIV OF SCI & TECH
Saccharomyces cerevisiae with high yield of salidroside as well as construction method and application of saccharomyces cerevisiae
The invention discloses saccharomyces cerevisiae with high yield of salidroside as well as a construction method and application of the saccharomyces cerevisiae. According to the recombinant saccharomyces cerevisiae, ribulose-5-phosphate isomerase RKI1 and transketolase TKL1 are expressed in host bacteria, so that precursor supply is increased; reverse methyltransferase ARO2 and phenylalanine decarboxylase ARO10 are expressed at the same time, and a tyrosine branch pathway is adjusted; then, UDP-glycosyl transferase of different sources is expressed, and iterative site-specific mutagenesis is carried out on the UDP-glycosyl transferase to enhance conversion of hydroxytyrosol to salidroside; the salidroside production performance of the recombinant strain is verified on the basis of a precursor supply pathway, a tyrosine regulation pathway and key enzyme screening, and the salidroside production capacity of the saccharomyces cerevisiae is further improved.
Owner:NANJING TECH UNIV
Ergothioneine-producing recombinant engineering bacterium as well as construction method and application thereof
The invention discloses an ergothioneine-producing recombinant engineering bacterium as well as a construction method and application thereof, and belongs to the technical field of gene recombination fermentation. The method comprises the following steps: by taking escherichia coli as a starting bacterium, carrying out recombinant expression on a histidine trimethyl inner salt cysteine sulfoxide synthetase egt1 gene, an L-histidine methyltransferase egtD gene, a histidine trimethyl inner salt cysteine sulfoxide lyase egt2 gene, a methionine adenosine transferase metK gene, an adenylate kinase adK gene and an adenine phosphoribose transferase apt gene; on this basis, supply of precursor substances including histidine, cysteine and methionine is further improved through metabolic transformation, the yield of the finally obtained recombinant engineering bacterium for producing ergothioneine reaches 2.54 g / L after 48 h of shake-flask culture, the yield of a 5L fermentation tank reaches 18 g / L after further enlarged culture, the yield of ergothioneine is guaranteed while energy consumption and cost are reduced, and the method is suitable for industrial production. The method is suitable for practical popularization.
Owner:SHANGHAI RECOM BIOTECHNOLOGY CO LTD
Artificial efficient nitrogen fixation system as well as preparation method and application thereof
The invention relates to an artificial efficient nitrogen fixation system and a preparation method and application thereof. The artificial efficient nitrogen fixation system contains recombinant engineering bacteria constructed by an artificial combination module and nitrogen fixation chassis microorganisms; wherein the artificial combination module sequentially comprises a constitutive promoter element Pc and a coding gene of methyltransferase selected from nitrogen-fixing pseudomonas stutzeri A1501 in the direction from upstream to downstream. According to the recombinant engineering bacteria constructed by introducing the artificial combination module into the nitrogen-fixing chassis microorganism, the nitrogenase activity of the nitrogen-fixing chassis microorganism can be remarkably improved, the nitrogen-fixing capacity of the nitrogen-fixing chassis microorganism is remarkably improved, and the recombinant engineering bacteria have a relatively good application prospect in the aspect of promoting plant growth.
Owner:THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
Extraction method and application of ananas comosus exosome
The present application relates to a kind of anaphalis contorta exosome extraction method and its application.The exosome is obtained by ultra-high speed centrifugation method from fresh anaphalis contorta, particle size is 160-200 nm, Zeta potential is-21.63±1.34 mV, with double membrane structure, mainly containing protein (such as resveratrol O-methyltransferase etc.) and phospholipid, polysaccharide content is only 12.5% of anaphalis contorta fresh medicine, and does not contain traditional active ingredient anaphalis contorta glycoside.Experiments show that anaphalis contorta exosome can be targeted to enrich in colorectal tissue, significantly improve AOM / DSS induced colorectal cancer model mouse tumor microenvironment, reduce tumor number, regulate T cell and macrophage ratio, and relieve pathological damage, better than anaphalis contorta fresh medicine.The mechanism is related to protein component, not polysaccharide or small molecule component.The anaphalis contorta exosome provided in the present application has simple preparation process, high safety, and has significant anti-colorectal cancer application potential.
Owner:ZHEJIANG CHINESE MEDICAL UNIVERSITY
Methyltransferase mutant and its application in production of icariin
The application provides a methyltransferase mutant and application thereof in production of icariin, and belongs to the technical field of bioengineering. The application solves the problem of low icariin yield in the prior art. The methyltransferase mutant is obtained by mutating any one of the 76th position, the 146th position, the 250th position and the 290th position of the amino acid shown in SEQ ID NO. 2. The methyltransferase mutant is mainly used for efficient methyltransferase and application thereof in synthesis of icariin.
Owner:QINHUANGDAO HUIEN BIOTECHNOLOGY CO LTD
Flavone-4 '-O-methyltransferase mutant as well as preparation method and application thereof
The invention belongs to the technical field of synthetic biology and enzyme engineering, and particularly relates to a flavone 4 '-O-methyltransferase mutant as well as a preparation method and application thereof. Specifically, wild type methyltransferase as shown in SEQ ID NO.1 is subjected to mutation modification, and a series of mutants with improved enzyme activity are obtained. The mutant enzyme is obtained by respectively performing point mutation and superposition mutation on a plurality of amino acid sites of a wild-type methyltransferase amino acid sequence, so that the capability of synthesizing icaritin by the methyltransferase is remarkably enhanced, and the highest yield is 11.4 times of that of the wild-type methyltransferase. Certain support is provided for industrial fermentation production of icaritin, and therefore the icaritin has good practical application value.
Owner:SHANDONG UNIV
Polypeptide inhibitor of targeted RNA methyltransferase dimer and application of polypeptide inhibitor
The invention belongs to the technical field of biological medicine, and discloses a polypeptide inhibitor of a targeted RNA methyltransferase dimer and application of the polypeptide inhibitor. The polypeptide inhibitor provided by the invention effectively inhibits the formation and functional activity of the heterodimer by targeting a binding site required by the assembly of the METTL3-METTL14 heterodimer, for example, the polypeptide inhibitor can significantly reduce the total m6A modification level in acute myelogenous leukemia (AML) cells, thereby inhibiting cell proliferation and inducing apoptosis. Experimental results show that the polypeptide inhibitor disclosed by the invention shows good anti-tumor activity in an AML (acute myeloid leukemia) cell line, and the level of marker protein related to AML can be remarkably reduced.
Owner:CHINA PHARM UNIV
A method for producing creatine by fermenting carbohydrate raw materials using microorganisms
The present invention discloses a method for producing creatine by fermenting saccharide raw materials using microorganisms, and belongs to the field of genetic engineering technology. L-arginine-glycine amidinotransferase and guanidineacetic acid N-methyltransferase with high enzyme activity are overexpressed in Corynebacterium glutamicum var.CCTCC AB 2021051 using the pXMJ19 plasmid to construct a creatine synthesis pathway. The degradation of SAH is promoted by integrating the expression of the SAHase enzyme, thereby relieving the inhibitory effect of SAH on the key enzymes in creatine production and promoting the regeneration of SAH substrate SAM. The engineered bacteria are scaled up in a 5L tank, fermented for 60 hours, and the creatine yield reaches 14.2g / L. The production method of creatine by the present invention has the advantages of low production cost and high efficiency, which is conducive to scaled-up production.
Owner:JIANGNAN UNIV