153 results about "Jasmonic acid methyl ester" patented technology

The invention discloses a method for increasing the content of mogroside V. The method comprises the following steps: step I, dissolving jasmonic acid methyl ester into an ethanol solution of which the volume percentage is 20%, and preparing a 50mmol/L ethanol solution of the jasmonic acid methyl ester; dissolving the jasmonic acid methyl ester into ethanol, and preparing a 50-400[mu] mol/L jasmonic acid methyl ester solution; step II, inoculating a momordica grosvenori tissue culture seedling into a solid culture medium, wherein ethanol mother liquor of the jasmonic acid methyl ester is added into the solid culture medium when the solid culture medium is prepared, and thus the final concentration of the jasmonic acid methyl ester is 50-400[mu] mol/L; step III, transplanting the tissue culture seedling into a field, 50-60 days after pollination of momordica grosvenori, spraying the jasmonic acid methyl ester solution once in the morning, at noon and at night every day till water drops off from leaves, and continuously spraying for 10 days. The method is capable of effectively increasing the yield of the mogroside V and has the characteristics of being rapid in effect taking, low in cost, simple to operate, convenient to implement, free of toxin or residue in products and the like, and large-scale production of the mogroside V can be met.

The invention relates to an environment-friendly prevention and control method of panax notoginseng diseases and belongs to the technical fields of plant disease prevention and control and plant cultivation. The prevention and control method comprises land selecting and soil preparation, panax notoginseng planting and seed handling and growing period management, wherein thoroughly-decomposed cow dung, begasses, compound fertilizers and plant ash are mixed according to the volume ratio of 5:4:1:1; 6000-10000kg/hm<2> of improved soil is adopted; xiandi, 1cm of marigold limbs and trichoderma harzianum are applied after ditching and less xiandi is used for mixing with roots before transplanting; crofton weed and the marigold limbs are covered on a moisture face after transplanting, bottom-ventilated row covers are constructed, two sides of the bottom-ventilated row covers are vertical to the ground, and the bottom-ventilated row covers are 15cm in length, 1.8m in width and 1.2m in arc height; the trichoderma harzianum and the marigold infusion are applied in a sprayed mode according to the ratio of 1:200 after emergence of seedling to irrigate roots, and 100g/L of jasmonic acid methyl ester are applied to leaf faces in a sprayed mode. The environment-friendly prevention and control method has the advantages of being simple in operation, low in cost and easy to popularize, protecting the environment, being environment-friendly and the like, lays a good foundation to pollution-free planting of the panax notoginseng and has good economic benefits, ecological benefits and social benefits.

The invention discloses a method for increasing the content of cucurbitadienol in momordica grosvenori. Jasmonic acid methyl ester is added in the tissue culture process and/or planting process of the momordica grosvenori. The method is capable of effectively increasing the yield of the cucurbitadienol in the momordica grosvenori, has the advantages of being rapid in effect taking, low in cost, simple to operate, convenient to implement, free of toxin or residue and the like, is capable of meeting large-scale production of the cucurbitadienol in the momordica grosvenori, and has relatively good practicability and high popularization values.

The invention provides a method for improving the yield of saponin in panax notoginseng tissue culture seedlings by applying jasmonic acid methyl ester. The method comprises the following steps: establishing a fast breeding system of panax notoginseng tissue culture seedlings, applying jasmonic acid methyl ester to pseudo-ginseng tissue culture seedlings, cultivating pseudo-ginseng tissue culture seedlings and applying and spraying jasmonic acid methyl ester to the leaf surfaces of pseudo-ginseng tissue culture seedlings. The method is simple, convenient and easy to implement, can enable a great number of good pseudo-ginseng fast breeding seedlings to be formed in a short time, and has the advantages of high breeding speed and short period and the like; the externally applied jasmonic acid methyl ester can effectively improve the yield of total panax notoginseng saponins by 15 times within 5-10 days, and can improve the yield of main monomer ginsenoside by 2-5 times. According to the method for improving yield of saponin in panax notoginseng tissue culture seedlings, the yield of notoginsenoside can be effectively improved, the panax notoginseng tissue culture seedlings are bred by adopting a solid cultivation method, expensive fermentation equipment is not needed, the investment is reduced, the culture method is simple to operate, the breeding coefficient is high, the breeding speed is high, the contamination rate is low, seasonal limitation is avoided, the production cost of notoginsenoside is effectively reduced, and a practical guiding significance is provided for mass production of notoginsenoside.

The invention discloses a method for cultivating fritillaria przewalskii test-tube bulblet and aims to solve the problems of low induction rate, long cultivation cycle and complicated steps of a conventional fritillaria cultivation method. The method comprises the following steps: selecting an explant to break fritillaria into cuiller and scale folus, sterilizing the explant, and then inducing the explant. The specific inducing method comprises the following steps: inoculating the sterilized explant into an inducing medium, cultivating under the dark condition that the temperature is 16 to 25 DEG C, and inducing protocorm at the base of the scale folus or the cuiller, wherein the inducing medium for the scale folus comprises the following formula: 50 to 80g/L of 1/2MS basic culture solution and sucrose, 2 to 5g/L of activated carbon, 5 to 6g/L of agar, 500 to 2,000mg/L of arginine, 1 to 5mg/L of zeatin (ZT), 0.1 to 0.5mg/L of brassinolide (BR) and 1 to 5mg/L of jasmonic acid methyl ester (MEJA), the hormone formula for the cuiller is different from that for the scale folus, and the pH value of the medium is 5.5 to 6.5. The method has the characteristics of high breeding speed, short cultivation cycle, high propagation rate and convenient transplantation, can be used for cultivating seed bulb, and can be used as a commodity.

The invention relates to the field of fruit and vegetable preservation and in particular relates to a biological preservation method for blueberry fruits. The method mainly comprises the steps of fumigating pre-cooled blueberries by jasmonic acid methyl ester. The method can be used for improving the resistance of the blueberry fruits, effectively preventing the rot of the fruits and prolonging the storage period of the fruits, is safe and efficient, and meets the food safety requirements; moreover, the blueberry fruits do not contain any preservative or chemical residues.

The invention discloses a method for increasing the content of mogroside IV. The method comprises the following steps: I, preparing a solid culture medium containing jasmonic acid methyl ester, wherein the concentration of the jasmonic acid methyl ester is 50-400 [mu] mol/L before the solid culture medium is cured; and II, inoculating momordica grosvenoritissue culture seedlings into the solid culture medium, culturing for 28-35 days, and transplanting the momordica grosvenori culture seedlings into fields. The method has the characteristics of being rapid in effect taking, low in cost, simple to operate, convenient to implement, free of toxin or reside in products, and the like, and by adopting the method, the yield of the mogroside IV can be effectively increased, and large-scale production of the mogroside IV can be achieved.

The invention discloses a compound spraying agent and application thereof in promotion of crop kernel filling and seed viability. The compound spraying agent is prepared from a component A and a component B according to the volume ratio of 1:(1-100), wherein the component A takes water as a solvent and contains 1-50 mM of spermidine, 1-15 mM of jasmonic acid methyl ester and 10-50 mM of ethanol, and the component B is a sweet corn young ear extracting solution. According to the compound spraying agent, spermidine and jasmonic acid methyl ester are taken as effective ingredients of the spraying agent and mixed with the sweet corn young ear extracting solution, and by spraying the spraying agent to leaf surfaces or female ears of crops at the appropriate concentration, dry matter accumulation of sweet corn kernels is effectively promoted, the thousand kernel weight of sweet corn is increased, and the harvested seed viability is improved.

The invention discloses a method for increasing content of ginsenoside Rb1 during pseudo-ginseng cultivation. The method comprises the following steps: utilizing a jasmonic acid methyl ester solution and a salicylic acid solution to spray pseudo-ginseng plants in an alternating mode 2-10 days before pseudo-ginseng harvest. By spraying the jasmonic acid methyl ester solution and the salicylic acid solution on pseudo-ginseng leaves in an alternating mode before pseudo-ginseng harvest, high expressions of key enzyme genes of metabolism of ginsenoside Rb1 in cultured pseudo-ginseng are induced, thereby rapidly increasing the content of ginsenoside Rb1 in the short time.

The invention discloses a fresh-keeping agent for preventing and treating tomato fruit postharvest early blight and an application method of the fresh-keeping agent. The method comprises the steps of (1) carrying out cleaning and sterilization on picked fresh tomato fruits; (2) preparing the fresh-keeping agent for preventing and treating tomato fruit postharvest early blight, wherein the fresh-keeping agent comprises jasmonic acid methyl ester, beta-aminobutyric acid, chitosan, citric acid, calcium chloride and water; (3) soaking the cleaned and sterilized tomato fruits in the fresh-keeping agent for preventing and treating tomato fruit postharvest early blight for 5-15 minutes; (4) taking out the soaked tomato fruits in a room-temperature condition and carrying out short-wave ultraviolet irradiation on the surfaces of the dried tomato fruits; and (5) storing the tomato fruits subjected to short-wave ultraviolet irradiation at room temperature. According to the fresh-keeping agent for preventing and treating tomato fruit postharvest early blight and the application method of the fresh-keeping agent, the early blight symptoms of the tomato fruits in the storage and transportation processes can be effectively relieved and the postharvest storage period is significantly prolonged.

The invention discloses a method for increasing the content of siamenoside I in momordica grosvenori. According to the method, jasmonic acid methyl ester is applied in a tissue culture or cultivation process of the momordica grosvenori. By virtue of the method, the yield of the momordica grosvenori siamenoside in the can be effectively increased. The method has the characteristics of fast effectiveness, low cost, simplicity in operation, and convenience in implementation, and the product has no toxicity or residue and the like, can meet the large-scale requirement of the momordica grosvenori siamenoside and has relatively high practicability and promotional values.

The invention discloses an inducible promoter PCHI which is derived from lilium regale. The nucleotide sequence of the inducible promoter PCHI is shown as SEQ ID NO: 1. According to the invention, technical research related to molecular biology and genetic engineering proves that the lilium regale promoter PCHI responds to jasmonic acid methyl ester and biological stress. An expression cassette constructed by the lilium regale promoter PCHI and a beta-glucosidase gene is transferred into tobacco for expression, and the glucosidase activity of the obtained transgenic tobacco is quantitatively detected through a fluorescence method; and results show that after the transgenic tobacco is treated with jasmonic acid methyl ester, fusarium oxysporum, fusarium solani and sporotrichum oryzae treatment, the glucosidase activity of the transgenic tobacco is obviously enhanced. The lilium regale promoter PCHI is induced by jasmonic acid methyl ester and biological stress factors and can be used in disease-resistant gene engineering of plants.

The invention relates to the technical field of fruit and vegetable fresh keeping, in particular to a postharvest storage and fresh keeping method for pitaya fruits. The method comprises the step that the harvested pitaya fruits are soaked in a jasmonic acid methyl ester solution and a methyl salicylate solution in sequence, and therefore the phenomena that matured fruits are rotten, softened and the like are obviously reduced. By the adoption of the method, the pitaya fruit fresh keeping time can be remarkably prolonged, and storage quality can be improved. After the pitaya fruits are stored at normal temperature for 15 days and stored at low temperature for 25 days, the rotting rate of the pitaya fruits is reduced to below 15%. The storage period of the fruits at normal temperature and low temperature is effectively prolonged, the flavor and quality of the pitaya fruits are not affected, and the method has the advantages that the comprehensive utilization rate is high, the pitaya fruits are safe to eat, the quality is high, and the production is environmentally friendly.

The invention discloses a composite water-retaining coated fertilizer for flowers and plants. The composite water-retaining coated fertilizer is prepared from the following raw materials in parts by weight: 3.4-3.6 parts of cyclodextrin, 36-38 parts of manioc waste, 24-26 parts of dregs of tea-oil tree fruit, 12-14 parts of chestnut shell powder, 7-9 parts of calcium nitrate, 3-4 parts of ammonium sulfate, 2-4 parts of lentinan, 2-3 parts of xanthan gum, 43-46 parts of tangelo fruitlet, 1.0-1.2 parts of dimethyl formamide, 2.4-2.8 parts of jasmonic acid methyl ester, 0.9-1.1 parts of polyving alcohol, a proper amount of a 50% ethanol solution, a proper amount of a 70% methanol solution and a proper amount of water. Since organic matters are used as additives of the coated fertilizer disclosed by the invention, structural improvement of a flower cultivation medium is achieved, and the surface tension of the medium is improved; due to addition of cyclodextrin, xanthan gum and the like, the fertilizer is relativelygood in water absorption property; compared with a common high-molecular compound water-retaining agent, the composite water-retaining coated fertilizer has the advantages of high degradation speed and low cost and is applicable to flower planting in drought regions.

The invention discloses a method for effectively delaying sprout tumbling at high temperature and raising yield and alkaloid content of pinellia ternate. During the growing process of pinellia ternate, 0.03-0.3mmol/L jasmonic acid methyl ester aqueous solution is sprayed from an external source, so that the sprout tumbling of pinellia ternate can be delayed by at most 9 days, the yield of pinellia ternate can be raised by 2%-5%, and the alkaloid content of pinellia ternate can be raised by 6%-38%. The method is easy to implement, convenient to operate, easy for pesticide farmers to master, convenient to promote, low in investment and high in profit.

The invention belongs to the field of crop fertilizer, in particular relates to synergistic ecological fertilizer special for radix astragali and a preparation method of the synergistic ecological fertilizer. The synergistic ecological fertilizer special for the radix astragali is prepared from the following components by a certain weight proportion: sorghum halepense stalks, Chinese pennisetum, palm leaves, wormcast, a starter culture, charcoal, amino-bentonite, phosphorite powder, urea, potassium sulphate, microelements, fructo-oligosaccharide and jasmonic acid methyl ester. The ecological fertilized provided by the invention takes natural organic matters as main materials, green and environmental protection is achieved, crops can be promoted to rapidly grow, meanwhile the fructo-oligosaccharide and the jasmonic acid methyl ester are added in the mixed fertilizer, a secondary metabolism process of the radix astragali is promoted, the content of astragalus polysaccharide is increased, and the quality of the radix astragali is improved. The synergistic ecological fertilizer is reasonable in formula, and overall and balanced in nutrition, the utilization rate of fertilizer effect can be obviously improved, the yield and quality of the radix astragali can be greatly improved, the soil environment can be improved by long-term application of the ecological fertilizer, the capacities of fertilizer conservation and fertilizer supply and the buffer capacity of the soil can be enhanced, the occurrence of damage by disease and insect can be reduced, and the economic benefit of the radix astragali can be improved.

The invention provides a method for simulating a biological stress reaction to induce gingkoes to quickly accumulate bilobalide, and belongs to the technical field of biological techniques and botanytechniques. Gingko plants are cultured through simulation of natural growth conditions, jasmonic acid methyl ester (MeJA), namely a plant hormone, is adopted for inducing, and a high-performance liquid chromatography-evaporation light scattering method is adopted for detecting and analyzing the dynamic change condition of accumulation of the bilobalide. The change conditions of the bilobalide content are detected under different inducing concentration and time conditions, and therefore the concentration and inducing time of MeJA are determined, so that the optimum inducing condition of the MeJA for inducing fast accumulation of the bilobalide is screened out, really effective bases are provided for increasing the yield of the bilobalide, and a scientific basis is provided for treatment before harvesting of ginkgo leaves.

The invention provides an accelerant for glume opening of a photo-thermo-sensitive genic male sterile rice line under high-temperature stress and a preparing method of the accelerant. The accelerant is prepared from citric acid, ethyl alcohol and a plant growth regulating substance, namely, jasmonic acid methyl ester, the three substances have the supplementary effect after being prepared into a solution, the solution is sprayed in the heading stage and can effectively promote glume opening of the photo-thermo-sensitive genic male sterile rice line under high-temperature stress, and the setting percentage and the seed yield after hybridization of the photo-thermo-sensitive genic male sterile rice line and a restoring line are increased.

The invention discloses an oil-resistant aging-resistant natural rubber cable material, which is prepared from the following components: natural rubber, cis-1,4-polyisoprene rubber, epoxidized natural rubber, poly 2, 6-dimethyl-1,4-phenylate, soyabean protein, propylene glycol alginate, chitosan, zinc oxide, stearic acid, sulphur, zinc methacrylate, microcrystalline cellulose, white carbon black, montmorillonoid, talcum powder, magnesium carbonate, nanometer titania, a mesoporous molecular sieve, chromium hemitrioxide, phosphomolybdic acid, methyl 2,2-dithienyl glycolate, a jasmonic acid methyl ester, an anti-aging agent, an accelerant, and 2,4-dinitrophenylhydrazine. The oil-resistant aging-resistant natural rubber cable material provided by the invention is high in strength, good in heat resistance and oil resistivity, and excellent in ageing resistance and flame retardant property.

The invention discloses a cold-resistant anti-corrosion preservation technology for cold-sensitive fruits and vegetables after being harvested, and particularly relates to a cold-resistant preservative for cold-sensitive fruits and vegetables after being harvested and a preservation method thereof. The preservative disclosed by the invention comprises methyl jasmonate (2.0-10.0mg/L) and a mixed solution, wherein the mixed solution is an ice water solution containing salicylic acid, ascorbic acid, CaCl2 and an active agent Tween 80. The preservation method comprises the steps of firstly, carrying out sealed fumigation on the cold-sensitive fruits and vegetables for 12 hours by jasmonic acid methyl ester at room temperature, soaking and carrying out cold shock on the fruits and vegetables for 1 hour in the ice water mixed solution, taking out the fruits and vegetables and airing the surface water. The cold-resistant preservative does not generate obvious symptoms of chilling injury after being placed for two weeks in an environment at 0-4 DEG C; corruption mildew and quality drop of the fruits and vegetables stored in the environment at room temperature and proper low temperature can be effectively restrained; and the refreshing time is obviously prolonged.

The invention discloses a method for increasing the content of mogrol in momordica grosvenori. The method is characterized in that jasmonic acid methyl ester is added in the tissue culture process or planting process of the momordica grosvenori. The method is capable of effectively increasing the yield of the mogrol, has the characteristics of being rapid in effect taking, low in cost, simple to operate, convenient to implement, free of toxin or residue in products and the like, is capable of meeting large-scale production of the mogrol, and has relatively good practicability and high popularization values.

The invention discloses a preparation method for selenium-rich astaxanthin chlorella powder. The method comprises the following steps: selecting fine chlorella strain bred with ultraviolet ray and performing irradiation mutagenic treatment, thereby acquiring a fine new strain; culturing in a creative optimized BG11 culture medium; using a series of parallel 40.0W red light tubes and 1.2m long 28.0W blue light tubes for irradiating the chlorella liquid, continuously culturing till reaching the logarithmic phase and determining D600nm; inducing the chlorella to generate selenium-rich protein; adopting 1.2m long blue light tubes, high luminous intensity, nitrogen starvation, 51.8g/L glucose and 20.0g/L xylose for performing biomass fermentation and inducing a part of astaxanthin; increasing the illumination intensity in steps in a wide inducing stage; adopting jasmonic acid methyl ester, salicylic acid and ethephon for inducing, thereby acquiring astaxanthin selenium chlorella strain; lastly, adopting a creative combined enzymic method and the optimized high-pressure homogeneous wall breaking for acquiring the chlorella powder and adopting novel shrimp head endogenous enzyme for assisting in extracting astaxanthin.

The invention discloses a coated flower fertilizer with the bactericidal and insecticidal effects. The coated fertilizer is prepared from, by weight, 3-4 parts of dioscin, 46-48 parts of guano, 12-14 parts of fresh orange peel, 9-10 parts of leaf mold, 4-5 parts of trisodium phosphate, 7-9 parts of ammonuim humate, 2-3 parts of glucolactone, 1-2 parts of oxidized starch, 39-43 parts of young tangelo fruits, 0.6-0.8 part of dimethylformamide, 1.8-2.0 parts of jasmonic acid methyl ester, 1.1-1.4 parts of polyvinyl alcohol, an appropriate amount of a 50% ethanol solution, an appropriate amount of a 70% methanol solution and an appropriate amount of water. According to the coated fertilizer, the nutrient content is high, the added active components such as dioscin have the good insect expelling effect, and the blended fermented materials of the fresh orange peel, the leaf mold and the like can promote reproduction of an effective microbial community of a root system and prevent proliferation and infection of pathogenic bacteria; meanwhile, the adopted raw materials are all easy to degrade, residual pollutants cannot be caused, the pesticide resistance is not prone to generate, and an ideal usage effect on flower disease prevention and inhibition is achieved.