128 results about "Lipopeptide" patented technology

The application belongs to the technical field of daily chemical industry, and particularly relates to an emulsion and a preparation method thereof. The emulsion provided in the application comprises an emulsifier, glycerol, oil-phase active substance, pentaerythritol tetra(ethylhexanoate) and water; the emulsifier is a lipopeptide; the weight percentage of the lipopeptide and the glycerol is not less than 3%; the weight ratio of the oil-phase active substance and the pentaerythritol tetra(ethylhexanoate) is 1:3-10; the ratio of the sum of the weights of the lipopeptide and the glycerol to the sum of the weights of the oil-phase active substance and the pentaerythritol tetra(ethylhexanoate) is 1:3-10; and the ratio of the sum of the weights of the lipopeptide, the glycerol, the oil-phase active substance and the pentaerythritol tetra(ethylhexanoate) to the weight of the water is 1:1-4. The emulsion is prepared by using sodium bacillomycin, glycerol and pentaerythritol tetra(ethylhexanoate), can effectively inhibit the diffusion of the oil-phase active substance at the interface of the emulsion, reduce the oxidative degradation of the oil-phase active substance, and greatly prolong the effective action time of the oil-phase active substance in the emulsion.

The invention relates to bacillus for producing lipopeptide, bacteriostatic lipopeptide of the bacillus and application of the bacillus to aquatic products, and belongs to the technical field of microorganisms. The bacillus velezensis is a bacillus velezensis strain FJAT-57093, is preserved in the China General Microbiological Culture Collection Center on April 1, 2025, and has a preservation number of CGMCC No. 34063, and the bacillus velezensis strain FJAT-57093 is a bacillus velezensis strain FJAT-57093, is preserved in the China General Microbiological Culture Collection Center on April 1, 2025, and has a preservation number of CGMCC No. 34063. The bacteriostatic lipopeptide is prepared from three types of lipopeptides, namely, iturin, fengycin and surfactin. The invention further discloses a preparation method of the bacteriostatic lipopeptide. The bacillus and the bacteriostatic lipopeptide have a broad-spectrum bacteriostatic effect on aquatic pathogenic bacteria, can be used for aquaculture, and have a wide application prospect.

The invention relates to a method for improving fermentation production efficiency of bacillus subtilis lipopeptide sodium by controlling threonine flow acceleration rate based on fermentation process oxygen uptake rate curve feedback. Bacillus subtilis is used as a production strain, and lipopeptide sodium is produced through deep liquid state fermentation. In the fermentation process, the oxygen uptake rate of the process is detected, a threonine and ammonium sulfate continuous feeding control process based on oxygen uptake rate curve parameters is implemented, the oxygen uptake rate in the fermentation process is stably maintained to be 60-65 mmo1 / L / h, the ammonium ion concentration is maintained to be 15-20 mmol / L, and rapid growth of hyphae and rapid increase of the lipopeptide sodium synthesis rate can be achieved. The threonine and ammonium sulfate fed-batch control process based on oxygen uptake rate feedback control is established, the production period is shortened, and the fermentation production level is stably improved.

The invention discloses recombinant bacillus subtilis with high yield of surfactin as well as a construction method and application of the recombinant bacillus subtilis, and belongs to the technical field of genetic engineering. According to the invention, non-essential genes are determined through analysis and identification, a simplified engineering strain is obtained, and the highest fermentation yield reaches 11.2 g / L; then, a PTS part sugar phosphorylase gene obtained through screening is transferred into the simplified engineering strain, the metabolism efficiency of carbohydrates is improved, transportation and utilization of the carbohydrates are optimized, a fatty acid transmembrane transporter gene is further transferred, fatty acid uptake is assisted, synthesis of surfactin is facilitated, and the yield of the surfactin is improved. The combination of the two realizes optimization of synthesis of surfactin through a composite carbon source. Compared with a simplified engineering strain, the genetically engineered bacterium obtained by the invention has the advantages that the yield of surfactin is increased by 57.1%, the yield is increased by 2.3 times compared with that of an unsimplified engineering strain EX, and the final shake-flask fermentation yield can reach 17.6 g / L. As an industrial chassis cell of high-yield lipopeptide, the cell has a good industrial application prospect.

The invention provides a high-temperature-resistant lipopeptide reinforced foam flooding system and a screening method and application, and relates to the technical field of microbial oil recovery, the high-temperature-resistant lipopeptide reinforced foam flooding system comprises the following steps: S1, screening applied oil reservoirs; s2, based on the oil reservoir temperature and the crude oil viscosity selected in S1, screening a high-temperature-resistant lipopeptide reinforced foam flooding system; the high-temperature-resistant lipopeptide reinforced foam oil displacement system comprises a foaming agent, a foam stabilizer, gas and water, the foaming agent comprises one or more of surfactin (C12-18), scabrin (C14-18), iturin (C14-17), deocytin, serratia marcescens and mucin fermentation liquor, and the foaming agent comprises one or more of lipopeptide, a foaming agent and a foaming agent. The foam stabilizer comprises one or more of biosynthetic nano-silver, nano-iron, nano-copper, nano-zinc, nano-zirconium and nano-nickel. The method has the beneficial effects that the oil reservoir application range is wide, the pertinence is high, the field test effect is good, and a high-temperature-resistant lipopeptide enhanced foam oil displacement system is specifically constructed according to the temperature-resistant stability characteristics of lipopeptide surfactants and biosynthetic nano materials of different structures and types.

This application discloses a thickening composition and an amino acid surfactant, including a lipopeptide biosurfactant. The lipopeptide biosurfactant possesses the basic cleaning and foaming properties of surfactants while thickening the amino acid surfactant and its compound system, synergistically improving cleaning ability without affecting the system's appearance or transparency. The lipopeptides in this application are produced by bacteria, are a green source of raw materials, are mild and non-irritating, harmless to humans and environmentally friendly, biodegradable, and pose no harm with long-term use.

The present invention relates to a cyclic peptide, a conjugate comprising said cyclic peptide and a lipopeptide building block, a bundle of said conjugates, a synthetic virus-like particle comprising at least one bundle of conjugates and pharmaceutical compositions comprising the same. The present invention further relates to said cyclic peptide, said conjugate said bundle of conjugates, said synthetic virus-like particle and said pharmaceutical compositions for use as a medicament, preferably for use in a method for preventing of an infectious disease or reducing the risk of an infectious disease, more preferably for use in a method for preventing or reducing the risk of an infectious disease associated with or caused by a respiratory syncytial virus.

The invention belongs to the technical field of biological extracts and cosmetics, and discloses a leukocyte extract composition as well as a preparation method and application thereof. The leukocyte extract composition comprises the following components in percentage by mass: 5-20% of a leukocyte extract; 3-10% of sodium surfactin; 10-20% of a humectant; and 50-82% of water. The preparation method of the leukocyte extract composition comprises the following preparation steps: adding the sodium surfactin into hot water at 50-80 DEG C for dissolving, then adding the humectant, homogenizing, stirring and uniformly mixing, cooling to room temperature, then adding the leukocyte extract, stirring and uniformly dissolving to obtain the leukocyte extract composition. According to the composition, a specific cyclic lipopeptide compound, namely, sodium surfactin, is adopted as a synergistic stable synergist of the leukocyte extract, so that the activity stability of the leukocyte extract can be remarkably improved, and better and more stable anti-aging, repairing and whitening effects can be achieved under the condition of smaller addition amount.

The present invention belongs to the technical field of daily chemical industry, and specifically relates to an emulsion and a preparation method thereof. The emulsion provided by the present invention comprises an emulsifier, glycerol, an oil-phase active substance, pentaerythrityl tetra(ethylhexanoate) and water; wherein the emulsifier is a lipopeptide; the weight percentage of the lipopeptide relative to glycerol is not less than 3%; the weight ratio of the oil-phase active substance to pentaerythrityl tetra(ethylhexanoate) is 1:(3 to 13); the ratio of the total weight of the lipopeptide and glycerol to the total weight of the oil-phase active substance and pentaerythrityl tetra(ethylhexanoate) is 1:(3 to 10); and the ratio of the total weight of the lipopeptide, glycerol, oil-phase active substance and pentaerythrityl tetra(ethylhexanoate) to the weight of water is 1:(1 to 4). By compounding sodium surfactin with glycerol and pentaerythrityl tetra(ethylhexanoate), the emulsion can effectively inhibit the diffusion of the oil-phase active substance at an emulsion interface, reduce the oxidative degradation of the oil-phase active substance, and greatly prolong the effective action time of the oil-phase active substance in the emulsion.

The application discloses a floating composite microbial preparation for removing marine oil pollution and a preparation method thereof. The floating composite microbial preparation contains bacillus thuringiensis NH26 strain, hollow glass microbeads and a lipopeptide surfactant purified from a fermentation liquor of the NH26 strain, has floating characteristics, can rapidly activate and efficiently degrade oil hydrocarbon, has good degradation capacity for alkanes and polycyclic aromatic hydrocarbons in seawater, and still has good degradation capacity for alkanes in seawater containing high-concentration oil hydrocarbon. The floating composite microbial preparation can be used for removing marine oil pollution and repairing marine environment. In addition, the application further provides a preparation method of the floating composite microbial preparation. The preparation method has simple preparation process, the obtained floating composite microbial preparation has uniform particle size, has high embedding rates for nitrogen and phosphorus, and can be prepared on a large scale.

The invention provides a composite quality control product of an AIDS antibody, a syphilis antibody and a hepatitis C antibody as well as a preparation method and application of the composite quality control product. The composite quality control product comprises an AIDS (Acquired Immune Deficiency Syndrome) antibody, a syphilis antibody, a hepatitis C antibody and the following components: a matrix solution, 1.5 to 3 g / L of Pluronic F68, 0.05 to 0.15% v / v of ProClin 300, 0.1 to 1 g / L of sodium surfactin, 1 to 10 g / L of bovine serum albumin, 1 g / L of alanine, 2 to 5 g / L of trehalose, 0.5 to 1.0 g / L of EDTA (Ethylene Diamine Tetraacetic Acid) and 2 to 5 mmol / L of benzamidine. The matrix liquid comprises a buffer solution and 20-30% v / v human serum. According to the invention, the aggregation and inactivation risk of the antibody in the freezing and thawing process is effectively reduced, the antibody can be repeatedly frozen and thawed for more than three times, the degradation of the antibody at high temperature can be effectively prevented, and the reliability of the quality control product in the transportation and clinical use process is ensured.

The invention discloses bacillus subtilis for producing lipopeptide sodium and application of the bacillus subtilis, and belongs to the technical field of microbial engineering. The preservation number of the bacillus subtilis is CGMCC (China General Microbiological Culture Collection Center) No.34998 The strain is only subjected to basic fermentation, the yield of the lipopeptide sodium can reach 19.3 g / L, four lipopeptide sodium analogues can be synthesized in the fermentation process, the abundance of products is improved, and meanwhile, a new biological resource is provided for industrial and efficient production of the lipopeptide sodium. When the bacillus subtilis 2KR-18.1.13-26 provided by the invention is used for producing the lipopeptide sodium, the production efficiency of the lipopeptide sodium can be remarkably improved by optimizing a fermentation process, the economic benefit is considerable, and the bacillus subtilis 2KR-18.1.13-26 has relatively high implementation, popularization and application values.

This invention discloses a cyclic lipopeptide delivery system, comprising a cyclic lipopeptide carrier and nucleic acid; the head of the cyclic lipopeptide carrier is composed of heptapeptides R1R2R2R3R4R2R2 linked together by amide bonds to form a ring, and the hydrophobic tail of the cyclic lipopeptide carrier is composed of a fatty acid with a carbon chain length of 6-44 forming an ester bond with the hydroxyl group on the head amino acid R3, with the following general structural formula: where R1 is a basic amino acid, including one of histidine (His), lysine (Lys), or arginine (Arg); R2 is a hydrophobic amino acid, including one of leucine (Leu), isoleucine (Ile), or valine (Val); R3 is a hydrophilic amino acid, including one of tyrosine (Tyr), serine (Ser), or threonine (Thr); R4 is one of glutamate (Glu) or glutamine (Gln); n is an integer from 5 to 43; the cyclic lipopeptide carrier encapsulates the nucleic acid drug to form cyclic lipopeptide-nucleic acid nanoparticles.

Provided herein are a lipopeptide building block comprising a lipid moiety, an amphipathic domain, a hydrophilic polymer block, a protease cleavage site, and lipopeptide constructs, lipopeptide aggregates and immunogenic compositions comprising the same.

The invention relates to a complex microbial inoculant and application thereof in prevention and control of tobacco black shank and tomato spotted wilt, and belongs to the technical field of prevention and control of tobacco diseases. The complex microbial inoculant comprises antibacterial lipopeptide and bacillus subtilis, wherein the mass ratio of the antibacterial lipopeptide to the bacillus subtilis is (0.5-1.2): 10; 0.08 wt% to 0.12 wt% of antibacterial lipopeptide powder is adopted as the antibacterial lipopeptide; the antibacterial lipopeptide comprises at least one of surfactin, iturin and abinogen; the viable count of the bacillus subtilis is greater than or equal to 2 * 10 < 9 > CFU / g. According to the invention, a combined prevention and control method taking antibacterial lipopeptide and bacillus subtilis as cores is adopted, so that the synergistic prevention and control of tobacco black shank and tomato spotted wilt are realized, and meanwhile, the agricultural and economic characters of tobacco are improved; due to the dual effects of the bacillus subtilis and the antibacterial lipopeptide, the lasting period can be prolonged, and the stability is high; the complex microbial inoculant can be completely biodegraded and is suitable for organic agriculture.

The invention belongs to the technical field of cosmetics, and particularly relates to a shea butter composition as well as a preparation method and application thereof. The shea butter composition is prepared from the following raw materials in percentage by weight: 0.1 to 35 percent of shea butter, 0.01 to 0.5 percent of ceramide, 0.01 to 0.3 percent of sodium bacillus subtilis lipopeptide, 10 to 50 percent of glycerol and the balance of water. The shea butter composition has the beneficial effects that the shea butter composition has better moisturizing and repairing effects on skin, a surfactant used in the preparation process of the shea butter composition is far lower than that used in a traditional emulsion, and the shea butter composition has better popularization advantages; when applied to cosmetics, the product hardly promotes transdermal absorption of harmful substances such as preservatives, and is especially suitable for pregnant women, infants, people with sensitive skin and the like.

This invention belongs to the field of biomedical technology and relates to a drug-loaded vesicle for promoting the healing of diabetic wounds. The drug-loaded vesicle has a core-shell structure. The core contains siRNA targeting ALKBH5 mRNA; the shell is a hybrid membrane of human umbilical vein endothelial cell membrane and milk-derived extracellular vesicle membrane, with its surface embedded with arginine-rich lipopeptides, and at least some arginine exposed outside the hybrid membrane. The core-shell structure effectively protects the siRNA from degradation and reduces off-target interference. The hybrid membrane shell has good biocompatibility, immunogenicity, and low cytotoxicity. The exposed arginine enhances tissue penetration and cellular uptake. In vitro and in vivo experiments have confirmed that this drug-loaded vesicle has good cellular uptake efficiency, effectively downregulates ALKBH5 expression, and restores mRNA levels. 6 A-modification increases the stability of C2orf69 mRNA, improves endothelial cell function, significantly promotes the healing of diabetic wounds, and has good biocompatibility and safety.

The invention provides a composition for preventing and treating plant diseases and application thereof. The composition comprises antibacterial lipopeptide and a protein plant immunity inducer. Synchronous and efficient prevention and control of fungal black shank and viral PVY and CMV diseases are achieved for the first time, the prevention effect reaches 88% or above, and the industrial problem that the prevention and control spectrum of a single agent is narrow is solved.

The invention relates to the technical field of microbial fermentation, and discloses bacillus subtilis, application of the bacillus subtilis in fermented soybean meal and a preparation method of the fermented soybean meal. The bacillus subtilis screened by the invention has strong anti-nutritional factor resistance (such as glycinin, beta-conglycinin, raffinose and stachyose), has a good growth effect in the soybean meal raw material, and is used for fermenting the soybean meal raw material; the content of anti-nutritional factors (such as glycinin, beta-conglycinin, raffinose and stachyose) in the soybean meal raw material can be obviously reduced, and meanwhile, the content of lipopeptide substances (such as surfactin and bacitracin D) in the fermented soybean meal product can be obviously improved; the prepared fermented soybean meal product has a good antibacterial effect on three common intestinal pathogens (escherichia coli, salmonella and staphylococcus) in animal bodies.

The invention discloses a bacillus amyloliquefaciens Jcc3 and application thereof in prevention and treatment of aquatic diseases, the classification name of the bacillus amyloliquefaciens Jcc3 is bacillus amyloliquefaciens, the bacillus amyloliquefaciens Jcc3 is preserved in China Center for Type Microbiological Culture Collection, the preservation time is January 25, 2024, and the preservation number is CCTCC NO: M 2024210. The bacillus amyloliquefaciens Jcc3 disclosed by the invention is identified as a gram positive bacterium. The strain can produce lipopeptides, proteins and other substances with bacteriostatic action, and has a significant inhibitory effect on aquatic disease aeromonas veronii. By inhibiting the growth and reproduction of the aeromonas veronii, aquatic diseases caused by the aeromonas veronii can be effectively prevented and controlled, so that the immunity of aquatic animals is enhanced, and the death rate of the aquatic animals is reduced. In conclusion, the bacillus amyloliquefaciens Jcc3 has great application potential in prevention and control of aquatic diseases, especially in prevention and control of diseases caused by aeromonas veronii.

The invention belongs to the technical field of microorganisms, and particularly discloses a bacillus aryabhattai strain and application thereof, the bacillus aryabhattai strain is identified as the bacillus aryabhattai strain through morphology, physiology, biochemistry and 16S rDNA (ribosomal deoxyribonucleic acid) molecules, is named as GR-6, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No.37494. The bacillus aryabhattai GR-6 disclosed by the invention has an in-vitro control effect of 67.58% and a living potting control effect of 38.67% on tomato gray leaf spot disease, and has good nitrogen fixation and potassium solubilization capabilities, and generated volatile substances have an obvious inhibition effect on various plant pathogenic fungi such as Stemphylium solani, Corynespora cassiicola, Botryis cinerea, fusarium oxysporum and Colletotrichum capsici; according to the present invention, the genome contains Surfactin, Macrolactin, Difficidin and other lipopeptide and polyketone antibiotic synthesis related genes, such that the biocontrol effect is significant, the growth promoting function and the broad-spectrum antibacterial ability are provided, the environmental protection is provided, the new microbial resource is provided for the green prevention and control of the tomato gray leaf spot disease, and the important application development value is provided.

This invention discloses a lipopeptide HIV membrane fusion inhibitor and its pharmaceutical use. The lipopeptide of this invention is of formula I: X1-EMTWEEWEK KVEELEKKIEELLK-X2-X3-X4-X5; or formula II: X1-ELTWEEWEKKVEELEKKIEELLK-X6-X7-X4-X5; wherein, X1 is an amino-terminal protecting group; X2 is a polypeptide sequence (EAAAK)n, A[(EAAAK)n]A, or (EP)n, where n represents the number of repetitions; X3 is Lys, Cys, Dap, Orn, Dab, Dah, or absent; X4 is a lipophilic compound group used to modify the C-terminus; X5 is a carboxyl-terminal protecting group; X6 is a polypeptide sequence KAEEQQKKNE; and X7 is Lys, Cys, Dap, Orn, Dab, or Dah. The lipopeptide of this invention has higher antiviral activity.

The invention relates to the technical field of agricultural seed coating, in particular to a seed coating material containing byproduct yeast and polyglutamic acid as well as a preparation method and application of the seed coating material. A protein extract from byproduct yeast is used as a main molecular skeleton, and the adhesive force, the structure supporting property and the nutrition slow-release function are excellent; gamma-polyglutamic acid is compounded to serve as a functional synergist, and the continuity, flexibility and water retention capacity of the film layer are enhanced by means of high hydrophilicity and cohesiveness of the gamma-polyglutamic acid; lipopeptide is also introduced into the system to improve the stress resistance of the seeds, and biogenic additives such as chitosan and glycerol are supplemented to jointly construct a compact, stable and multifunctional integrated seed coating material. The product is environment-friendly and sustainable, and the seed germination rate and the seedling survival rate can be remarkably increased.

The invention provides a resorcinol derivative phospholipid complex dispersion as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. A resorcinol derivative, an antioxidant, lecithin, sodium surfactin and water are used as raw materials, a strongly hydrophobic phospholipid compound is formed through interaction between hydrophilic ends of the lecithin and strong molecules of phenolic hydroxyl groups of the resorcinol derivative, then a hydrophilic cyclopeptide layer is modified on the surface of the phospholipid compound through the sodium surfactin surfactin, and the hydrophobic phospholipid compound is prepared. The dispersity of the phospholipid complex in water is improved, the stability of the resorcinol derivative can be effectively improved, and the problem of high-temperature discoloration is avoided. The particle size of the resorcinol derivative phospholipid complex dispersion provided by the invention is less than 200nm, and the resorcinol derivative phospholipid complex dispersion has good hydrophilicity and better transdermal permeation absorption effect.

The invention provides a novel antiviral polypeptide, a lipopeptide, a preparation method thereof, a pharmaceutical composition, a pharmaceutical composition and application. The lipopeptide provided by the invention is obtained by conjugating an antiviral polypeptide, an optional linking arm, an amino acid residue and a lipophilic compound. The lipopeptide provided by the invention has the effect of resisting novel coronavirus.

This invention belongs to the field of environmental disinfectant technology, specifically relating to a disinfectant for animal husbandry environments and its preparation method. This invention induces Bacillus subtilis fermentation through a compound extract of hawthorn, codonopsis, and astragalus. The three extracts synergistically upregulate the expression of genes related to antibacterial lipopeptide synthesis, significantly enhancing the antibacterial potency of the fermentation broth. The highly active antibacterial fermentation broth is then incorporated into a sodium alginate-xanthan gum composite gel network. Utilizing the slow oxidation properties of ascorbic acid under environmental humidity and oxygen, the gel network gradually undergoes proton dissociation, achieving a stable and long-lasting release of the antibacterial active ingredients. Simultaneously, xanthan gum swells upon contact with water to form a highly viscoelastic gel film, significantly enhancing the disinfectant's adhesion and retention capacity on humid surfaces. The resulting disinfectant possesses comprehensive properties including high antibacterial activity, long-lasting action, and strong environmental adhesion. The preparation process is simple and controllable, making it suitable for large-scale application in livestock farming environments.

This application belongs to the technical field of daily chemical industry, and specifically relates to emulsions and methods for preparing the same. The emulsion provided in this application contains an emulsifier, glycerin, an oil-phase active substance, pentaerythritol tetra(2-ethylhexanoic acid) ester, and water, wherein the emulsifier is a lipopeptide, the weight percentage of lipopeptide to glycerin is 3% or more, the weight ratio of the oil-phase active substance to pentaerythritol tetra(2-ethylhexanoic acid) ester is 1:3 to 13, the ratio of the total weight of lipopeptide and glycerin to the total weight of the oil-phase active substance and pentaerythritol tetra(2-ethylhexanoic acid) ester is 1:3 to 10, and the ratio of the total weight of lipopeptide, glycerin, oil-phase active substance, and pentaerythritol tetra(2-ethylhexanoic acid) ester to the weight of water is 1:1 to 4. By using this emulsion in combination with surfactant sodium, glycerin, and pentaerythritol tetra(2-ethylhexanoic acid) ester, the diffusion of oil-phase active substances at the emulsion interface is effectively suppressed, reducing oxidative degradation of oil-phase active substances and significantly extending the effective duration of the oil-phase active substances in the emulsion.

The invention relates to the technical field of biological medicine, in particular to functionalized lipopeptide and a preparation method thereof, a functionalized lipopeptide-assisted nucleic acid drug lipid nano delivery system and application thereof. The functionalized lipopeptide is a compound formed by functionalized polyethylene glycol-lipid and partially or completely exposed amino groups of the functionalized hybrid polypeptide through a click chemical reaction or a Michael addition reaction; the functionalized hybrid polypeptide is a compound formed by an esterification reaction of an N-hydroxysuccinimide-polyethylene glycol-group and partially or completely exposed amino groups of the functionalized polypeptide. The lipopeptide provided by the invention is simple in preparation process and low in toxicity, and the lipopeptide-assisted nucleic acid drug lipid nano delivery system can realize high-efficiency delivery of various nucleic acid molecules such as siRNA, mRNA and DNA in vitro and in vivo and has a treatment effect on various diseases.

The invention relates to the technical field of plant growth promotion, and particularly discloses a humic acid-fulvic acid composite plant growth promoter and application thereof. The accelerant is prepared from the following components in percentage by mass: 30 to 38 percent of fulvic acid compound, 4 to 8 percent of seaweed extract, 2 to 5 percent of marine animal source amino acid, 0.5 to 2 percent of chitosan oligosaccharide, 0.5 to 1.5 percent of microorganism source lipopeptide, 0.03 to 0.5 percent of plant growth composite regulator, 1.2 to 2.5 percent of osmotic regulator, 0.3 to 1.0 percent of salicylic acid, 2 to 5 percent of composite trace element and the balance of water. The fulvic acid, the seaweed extract, the amino acid, the chitosan oligosaccharide, the lipopeptide, the precisely compounded plant regulator and the nutrient elements generate a remarkable synergistic effect, the effect of the fertilizer is comprehensively superior to that of a single conventional fertilizer, crops can be effectively helped to cope with abiotic stress, the yield of the crops is increased, and the yield of the crops is increased. And the method has a wide popularization and application prospect.

A cleansing composition comprises one or more biosurfactants and one or more taurate surfactants. The biosurfactants may be selected from a glycolipid e.g. sophorolipids, a lipopeptide or a phospholi