222 results about "Lipopeptide" patented technology

The invention discloses a complex microbial inoculant for promoting growth of leguminous plants and application of the complex microbial inoculant, and relates to the technical field of fertilizers and biologication.The complex microbial inoculant is prepared from 25-35 parts of a microbial inoculant, 10-20 parts of a biological active agent, 10-15 parts of a biological activator and 30-55 parts of water. The endophyte 3G1 which is screened and separated from rice seeds and can promote the growth of leguminous roots can be completely and successfully planted in plants by utilizing the effects of preventing and treating plant diseases and / or promoting plant growth of microorganisms, and the growth of the plants is directly influenced; in combination with the functions of low temperature resistance, plant disease resistance and the like of other complex microbial inoculants, the compound microbial inoculants have growth promoting and disease preventing effects on leguminous plants; the biological active agent is lipopeptide, so that the spreading performance of plant leaves can be improved, the absorption of the complex microbial inoculant is promoted, the activity of the microbial inoculant is improved, and the crop yield is promoted to be increased by 3%; the biological activator can provide trace elements in the plant growth process, and is matched with the complex microbial inoculants for use, so that the growth of crop seedlings can be effectively promoted, the stress resistance of the crop seedlings is improved, and the transplanting survival rate of the seedlings reaches up to 98%.

The application belongs to the technical field of daily chemical industry, and particularly relates to an emulsion and a preparation method thereof. The emulsion provided in the application comprises an emulsifier, glycerol, oil-phase active substance, pentaerythritol tetra(ethylhexanoate) and water; the emulsifier is a lipopeptide; the weight percentage of the lipopeptide and the glycerol is not less than 3%; the weight ratio of the oil-phase active substance and the pentaerythritol tetra(ethylhexanoate) is 1:3-10; the ratio of the sum of the weights of the lipopeptide and the glycerol to the sum of the weights of the oil-phase active substance and the pentaerythritol tetra(ethylhexanoate) is 1:3-10; and the ratio of the sum of the weights of the lipopeptide, the glycerol, the oil-phase active substance and the pentaerythritol tetra(ethylhexanoate) to the weight of the water is 1:1-4. The emulsion is prepared by using sodium bacillomycin, glycerol and pentaerythritol tetra(ethylhexanoate), can effectively inhibit the diffusion of the oil-phase active substance at the interface of the emulsion, reduce the oxidative degradation of the oil-phase active substance, and greatly prolong the effective action time of the oil-phase active substance in the emulsion.

Optimization of the formula of a culture medium for industrially producing a Bacillus velezensis TCS001 fermentation broth and culture process conditions therefor. By using the culture medium and the culture process conditions, not only can the fermentation production cost be reduced, but also the effective viable bacterium count and the spore count in the Bacillus velezensis TCS001 fermentation broth can be improved, so that the fermentation broth can be used for industrial production and market promotion and application. Provided is a lipopeptide-compound-containing mixture separated from a Bacillus velezensis TCS001 fermentation broth. By further optimizing the formula of the culture medium for the Bacillus velezensis TCS001 fermentation broth and the culture fermentation process conditions, the output and yield of lipopeptide compounds in the fermentation broth are successfully improved, providing an efficient and cost-effective solution for the control of plant diseases.

The invention provides a synthetic anti-cancer lipopeptide and application thereof, and particularly provides a non-natural polypeptide comprising domains X1, X2 and X3, in which X1 is selected from C6-C20 fatty acid residues, X2 is selected from C6-C20 fatty acid residues, X3 is selected from C6-C20 fatty acid residues, and X3 is selected from C6-C20 fatty acid residues; x2 is selected from a fragment of a cell penetrating peptide (CPPs); x3 is selected from fragments of peptides that specifically bind to protein kinase 2 (CK2). Compared with the prior art, the polypeptide can more effectively inhibit CK2, has higher targeting property on RPS6 protein, has better tumor inhibition activity and anti-metastasis effect and higher safety, and can realize large-scale production.

In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, the disclosure, in one aspect, relates to lipid nanoparticles for use as drug delivery agents. The lipid nanoparticles are composed of lipopeptide conjugates, where a peptide is bonded to a lipid. The lipid nanoparticles described herein represent a new approach in drug delivery, addressing critical challenges in balancing biodegradability, biocompatibility, and organspecific targeting. The lipid nanoparticles can selectively target and deliver bioactive agents to specific tissues in a subject by modifying specific amino acids and ratios thereof in the peptide of the lipopeptide conjugate.

The invention discloses a preparation method of composite lipopeptide capable of preventing high-temperature inactivation, and belongs to the technical field of bioactive peptide preparation. The preparation method comprises the following steps: mixing and dissolving a modified phospholipid compound, cholesterol and chloroform, and performing rotary evaporation to obtain a lipid film; mixing and shaking the lipid film, the lipopeptide mixture and a phosphate buffer solution, and performing ultrasonic treatment to obtain a composite lipopeptide liposome suspension; and carrying out spray drying on the composite lipopeptide liposome suspension to obtain composite lipopeptide powder. A lipopeptide mixture obtained through fermentation is mixed with a modified phospholipid compound and cholesterol, a high-temperature-resistant composite lipopeptide liposome suspension is constructed, and after high-temperature spray drying, the obtained composite lipopeptide still has good antibacterial activity.

The invention relates to bacillus for producing lipopeptide, bacteriostatic lipopeptide of the bacillus and application of the bacillus to aquatic products, and belongs to the technical field of microorganisms. The bacillus velezensis is a bacillus velezensis strain FJAT-57093, is preserved in the China General Microbiological Culture Collection Center on April 1, 2025, and has a preservation number of CGMCC No. 34063, and the bacillus velezensis strain FJAT-57093 is a bacillus velezensis strain FJAT-57093, is preserved in the China General Microbiological Culture Collection Center on April 1, 2025, and has a preservation number of CGMCC No. 34063. The bacteriostatic lipopeptide is prepared from three types of lipopeptides, namely, iturin, fengycin and surfactin. The invention further discloses a preparation method of the bacteriostatic lipopeptide. The bacillus and the bacteriostatic lipopeptide have a broad-spectrum bacteriostatic effect on aquatic pathogenic bacteria, can be used for aquaculture, and have a wide application prospect.

The invention discloses bacillus tequilensis P61 and application thereof in prevention and treatment of kiwi fruit soft rot, and belongs to the field of biological prevention and treatment. The bacillus tequilensis P61 provided by the invention is preserved in the China Center for Type Culture Collection on September 5, 2024, and the preservation number of the bacillus tequilensis P61 is CCTCC (China Center for Type Culture Collection) NO: M 20241916. According to the invention, the lipopeptide antibacterial substance is prepared according to the bacillus tequilensis P61. Experimental results show that the bacillus tequilensis P61 and lipopeptide antibacterial substances have an outstanding prevention and treatment effect on the soft rot of the kiwi fruits, have a remarkable bacteriostasis effect on pathogenic bacteria of the soft rot of the kiwi fruits such as botryosphaeria dothidea, alternaria alternata and phomopsis, remarkably reduce the damage rate of the kiwi fruits, are green and safe, are harmless to people and livestock, and are worthy of popularization and application. No pollution is caused to the environment. The invention provides a new biocontrol strain for biological prevention and control of kiwi fruit diseases, and has a wide application prospect.

A mixture containing lipopeptide compounds is separated from bacillus velezensis TCS001 fermentation liquor, and it is found that the mixture containing the lipopeptide compounds and the five lipopeptide compounds have the activity of inhibiting plant pathogenic fungi and are more stable compared with the bacillus velezensis TCS001. And the method has wide application value in field disease prevention and control. By optimizing the formula of the bacillus velezensis TCS001 culture medium and the culture and fermentation process conditions, the yield and yield of lipopeptide compounds in the bacillus velezensis TCS001 fermentation liquor are increased.

The invention relates to the technical field of microorganisms, in particular to bacillus velezensis NEAU-L05, application of the bacillus velezensis NEAU-L05, antibacterial cyclic lipopeptide Iturin A and application of the antibacterial cyclic lipopeptide Iturin A. The bacillus velezensis NEAU-L05 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center, the address is Institute of Microbiology, Chinese Academy of Sciences, 3 #, 1 # yard, West Beichen Road, Chaoyang District, Beijing, the preservation date is December 19, 2024, and the preservation number is CGMCC No.33130. The bacillus velezensis NEAU-L05 disclosed by the invention can be used for preparing the bacillus velezensis NEAU-L05. The bacteriostasis rate of the bacterial strain to botrytis cinerea of phytopathogen is 67.2%. The bacteriostatic cyclic lipopeptide ItuinA separated from the strain can improve the storage performance of berries, and a basis is provided for development of multiple biocontrol microbial agents with excellent performance.

Described are lipopeptides that inhibit coronavirus fusion to a host cell. Thus a therapeutic for treating or preventing the common cold is described along with methods of inhibiting and / or treating an alphacoronavirus infection. The lipopeptides comprise a peptide unit comprising an amino acid sequence having a high degree of sequence identity to a sequence from the C-terminal heptad repeat of a betacoronavirus S protein, such as that of SARS-CoV-2.

The invention discloses a bacterial strain C5A-1 capable of producing lipopeptide and having a plant growth promoting function and application of the bacterial strain C5A-1, solves the technical problem of promoting plant growth, especially root length, plant height, stem diameter, leaf number, leaf area, underground dry weight and overground dry weight, and particularly discloses bacillus velezensis C5A-1 or a variant or a progeny thereof. The bacillus velezensis C5A-1 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.32880. When the bacteria or the fermented supernate thereof or the combination thereof is applied to plants, the growth of the plants can be promoted, and the bacteria can be used for industrial production.

The invention discloses an antibacterial composite lipopeptide mixed fermentation process, and belongs to the technical field of microbial cultivation, fermentation equipment adopted by the fermentation process comprises a fermentation tank and a motor, a rotating shaft is rotatably connected to the interior of the fermentation tank, a first gear is arranged at the bottom of the rotating shaft, the output end of the motor is connected with a second gear, and the second gear is meshed with the first gear; scraping strips are arranged on the side wall of the rotating shaft, a prism shaft is sleeved with the rotating shaft, mixing blades are arranged on the side wall of the prism shaft, and a transmission shaft is arranged on the surfaces of the mixing blades. When the mixing blades rotate around the axis of the rotating shaft for stirring, the mixing blades can do reciprocating motion in the vertical direction along the axis of the rotating shaft, so that the action stirring range of the mixing blades can be widened, dead corners and blind areas are avoided, the turbulence generation frequency in the lifting rotating process is increased, and the exchange and mixing effects of a culture solution are improved; a boundary layer generated by accumulation of fermentation metabolites of a single flora under the action of centrifugal force or gravity in the fermentation process of multiple florae can be broken.

The invention relates to a method for improving fermentation production efficiency of bacillus subtilis lipopeptide sodium by controlling threonine flow acceleration rate based on fermentation process oxygen uptake rate curve feedback. Bacillus subtilis is used as a production strain, and lipopeptide sodium is produced through deep liquid state fermentation. In the fermentation process, the oxygen uptake rate of the process is detected, a threonine and ammonium sulfate continuous feeding control process based on oxygen uptake rate curve parameters is implemented, the oxygen uptake rate in the fermentation process is stably maintained to be 60-65 mmo1 / L / h, the ammonium ion concentration is maintained to be 15-20 mmol / L, and rapid growth of hyphae and rapid increase of the lipopeptide sodium synthesis rate can be achieved. The threonine and ammonium sulfate fed-batch control process based on oxygen uptake rate feedback control is established, the production period is shortened, and the fermentation production level is stably improved.

The invention discloses recombinant bacillus subtilis with high yield of surfactin as well as a construction method and application of the recombinant bacillus subtilis, and belongs to the technical field of genetic engineering. According to the invention, non-essential genes are determined through analysis and identification, a simplified engineering strain is obtained, and the highest fermentation yield reaches 11.2 g / L; then, a PTS part sugar phosphorylase gene obtained through screening is transferred into the simplified engineering strain, the metabolism efficiency of carbohydrates is improved, transportation and utilization of the carbohydrates are optimized, a fatty acid transmembrane transporter gene is further transferred, fatty acid uptake is assisted, synthesis of surfactin is facilitated, and the yield of the surfactin is improved. The combination of the two realizes optimization of synthesis of surfactin through a composite carbon source. Compared with a simplified engineering strain, the genetically engineered bacterium obtained by the invention has the advantages that the yield of surfactin is increased by 57.1%, the yield is increased by 2.3 times compared with that of an unsimplified engineering strain EX, and the final shake-flask fermentation yield can reach 17.6 g / L. As an industrial chassis cell of high-yield lipopeptide, the cell has a good industrial application prospect.

The invention provides a high-temperature-resistant lipopeptide reinforced foam flooding system and a screening method and application, and relates to the technical field of microbial oil recovery, the high-temperature-resistant lipopeptide reinforced foam flooding system comprises the following steps: S1, screening applied oil reservoirs; s2, based on the oil reservoir temperature and the crude oil viscosity selected in S1, screening a high-temperature-resistant lipopeptide reinforced foam flooding system; the high-temperature-resistant lipopeptide reinforced foam oil displacement system comprises a foaming agent, a foam stabilizer, gas and water, the foaming agent comprises one or more of surfactin (C12-18), scabrin (C14-18), iturin (C14-17), deocytin, serratia marcescens and mucin fermentation liquor, and the foaming agent comprises one or more of lipopeptide, a foaming agent and a foaming agent. The foam stabilizer comprises one or more of biosynthetic nano-silver, nano-iron, nano-copper, nano-zinc, nano-zirconium and nano-nickel. The method has the beneficial effects that the oil reservoir application range is wide, the pertinence is high, the field test effect is good, and a high-temperature-resistant lipopeptide enhanced foam oil displacement system is specifically constructed according to the temperature-resistant stability characteristics of lipopeptide surfactants and biosynthetic nano materials of different structures and types.

The present invention discloses a cyclic lipopeptide carrier for encapsulating nucleic acid drugs, comprising a cyclic lactone structure formed by a heptapeptide R1R2R2R3R1R2R2 and a β-hydroxy fatty acid with a carbon chain length of 6 to 44, and its general structural formula is as follows: #imgabs0# wherein R1 is a basic amino acid, including one of histidine His, lysine Lys or arginine Arg, R2 is one of the hydrophobic amino acids leucine Leu, isoleucine Ile or valine Val, and R3 is one of any amino acid; n is an integer of 2 to 40; the positively charged basic amino acids in the cyclic lipopeptide bind to the negatively charged nucleic acid drug through electrostatic interaction to form cyclic lipopeptide-nucleic acid particles. The cyclic lipopeptide carrier for encapsulating nucleic acid drugs provided by the present invention has high safety, can effectively protect nucleic acid drugs, improves their stability, and has good transfection efficiency for a variety of cells, and has broad application prospects in the field of nucleic acid drug delivery.

The invention relates to the technical field of oil displacement, in particular to an anti-calcium bio-based surfactant for oil displacement, which comprises the following components in parts by mass: 2-3 parts of nano biological particles; 4 to 6 parts of lipopeptide; 12 to 15 parts of octadecyl dimethyl hydroxypropyl sulphobetaine; 0.5 to 1 part of a coupling agent; 92 to 97 parts of deionized water; the preparation method comprises the following steps: S1, dissolving lipopeptide and octadecyl dimethyl hydroxypropyl sulfobetaine which are subjected to ultrasonic sensitization in deionized water to obtain a mixed solution; s2, mixing the nano biological particles subjected to ultrasonic activation, a coupling agent and the mixed solution to obtain a bio-based surfactant; the bio-based surfactant prepared by the invention effectively resists interference of calcium on oil displacement performance, and maintains relatively low oil-water interfacial tension, proper system viscosity and good flowability, so that the oil displacement efficiency and calcium resistance are improved.

This application discloses a thickening composition and an amino acid surfactant, including a lipopeptide biosurfactant. The lipopeptide biosurfactant possesses the basic cleaning and foaming properties of surfactants while thickening the amino acid surfactant and its compound system, synergistically improving cleaning ability without affecting the system's appearance or transparency. The lipopeptides in this application are produced by bacteria, are a green source of raw materials, are mild and non-irritating, harmless to humans and environmentally friendly, biodegradable, and pose no harm with long-term use.

The invention relates to a pest control composition comprising a phenol, such as thymol, an aldehyde, such as vanillin, any one or more of the following: a free fatty acid, a fatty acid salt, and a lipopeptide, and a carrier and / or a solvent. The invention further relates to a pest control composition comprising thymol, an oil selected from the group consisting of rapeseed oil, soybean oil and diesel oil, a stabilizer and a solvent. The invention further relates to methods of preparing these pest control compositions and to uses of these compositions.

The invention relates to the technical field of cosmetics, in particular to a composition containing PQQ, application of the composition and a skin care product. The composition of pyrroloquinoline quinone or sodium salt thereof and sodium surfactin provided by the invention has good permeability and stability, and meanwhile, has a remarkable synergistic interaction effect in the aspects of oxidation resistance, whitening, bacteriostasis and the like. Wide application prospects are realized in the field of cosmetics. The preparation method of the composition is simple in steps, easy to operate and suitable for industrial production.

The present invention relates to a cyclic peptide, a conjugate comprising said cyclic peptide and a lipopeptide building block, a bundle of said conjugates, a synthetic virus-like particle comprising at least one bundle of conjugates and pharmaceutical compositions comprising the same. The present invention further relates to said cyclic peptide, said conjugate said bundle of conjugates, said synthetic virus-like particle and said pharmaceutical compositions for use as a medicament, preferably for use in a method for preventing of an infectious disease or reducing the risk of an infectious disease, more preferably for use in a method for preventing or reducing the risk of an infectious disease associated with or caused by a respiratory syncytial virus.

The invention belongs to the technical field of biological extracts and cosmetics, and discloses a leukocyte extract composition as well as a preparation method and application thereof. The leukocyte extract composition comprises the following components in percentage by mass: 5-20% of a leukocyte extract; 3-10% of sodium surfactin; 10-20% of a humectant; and 50-82% of water. The preparation method of the leukocyte extract composition comprises the following preparation steps: adding the sodium surfactin into hot water at 50-80 DEG C for dissolving, then adding the humectant, homogenizing, stirring and uniformly mixing, cooling to room temperature, then adding the leukocyte extract, stirring and uniformly dissolving to obtain the leukocyte extract composition. According to the composition, a specific cyclic lipopeptide compound, namely, sodium surfactin, is adopted as a synergistic stable synergist of the leukocyte extract, so that the activity stability of the leukocyte extract can be remarkably improved, and better and more stable anti-aging, repairing and whitening effects can be achieved under the condition of smaller addition amount.

The present invention belongs to the technical field of daily chemical industry, and specifically relates to an emulsion and a preparation method thereof. The emulsion provided by the present invention comprises an emulsifier, glycerol, an oil-phase active substance, pentaerythrityl tetra(ethylhexanoate) and water; wherein the emulsifier is a lipopeptide; the weight percentage of the lipopeptide relative to glycerol is not less than 3%; the weight ratio of the oil-phase active substance to pentaerythrityl tetra(ethylhexanoate) is 1:(3 to 13); the ratio of the total weight of the lipopeptide and glycerol to the total weight of the oil-phase active substance and pentaerythrityl tetra(ethylhexanoate) is 1:(3 to 10); and the ratio of the total weight of the lipopeptide, glycerol, oil-phase active substance and pentaerythrityl tetra(ethylhexanoate) to the weight of water is 1:(1 to 4). By compounding sodium surfactin with glycerol and pentaerythrityl tetra(ethylhexanoate), the emulsion can effectively inhibit the diffusion of the oil-phase active substance at an emulsion interface, reduce the oxidative degradation of the oil-phase active substance, and greatly prolong the effective action time of the oil-phase active substance in the emulsion.

The application discloses a floating composite microbial preparation for removing marine oil pollution and a preparation method thereof. The floating composite microbial preparation contains bacillus thuringiensis NH26 strain, hollow glass microbeads and a lipopeptide surfactant purified from a fermentation liquor of the NH26 strain, has floating characteristics, can rapidly activate and efficiently degrade oil hydrocarbon, has good degradation capacity for alkanes and polycyclic aromatic hydrocarbons in seawater, and still has good degradation capacity for alkanes in seawater containing high-concentration oil hydrocarbon. The floating composite microbial preparation can be used for removing marine oil pollution and repairing marine environment. In addition, the application further provides a preparation method of the floating composite microbial preparation. The preparation method has simple preparation process, the obtained floating composite microbial preparation has uniform particle size, has high embedding rates for nitrogen and phosphorus, and can be prepared on a large scale.

The invention provides a composite quality control product of an AIDS antibody, a syphilis antibody and a hepatitis C antibody as well as a preparation method and application of the composite quality control product. The composite quality control product comprises an AIDS (Acquired Immune Deficiency Syndrome) antibody, a syphilis antibody, a hepatitis C antibody and the following components: a matrix solution, 1.5 to 3 g / L of Pluronic F68, 0.05 to 0.15% v / v of ProClin 300, 0.1 to 1 g / L of sodium surfactin, 1 to 10 g / L of bovine serum albumin, 1 g / L of alanine, 2 to 5 g / L of trehalose, 0.5 to 1.0 g / L of EDTA (Ethylene Diamine Tetraacetic Acid) and 2 to 5 mmol / L of benzamidine. The matrix liquid comprises a buffer solution and 20-30% v / v human serum. According to the invention, the aggregation and inactivation risk of the antibody in the freezing and thawing process is effectively reduced, the antibody can be repeatedly frozen and thawed for more than three times, the degradation of the antibody at high temperature can be effectively prevented, and the reliability of the quality control product in the transportation and clinical use process is ensured.

The invention discloses bacillus subtilis for producing lipopeptide sodium and application of the bacillus subtilis, and belongs to the technical field of microbial engineering. The preservation number of the bacillus subtilis is CGMCC (China General Microbiological Culture Collection Center) No.34998 The strain is only subjected to basic fermentation, the yield of the lipopeptide sodium can reach 19.3 g / L, four lipopeptide sodium analogues can be synthesized in the fermentation process, the abundance of products is improved, and meanwhile, a new biological resource is provided for industrial and efficient production of the lipopeptide sodium. When the bacillus subtilis 2KR-18.1.13-26 provided by the invention is used for producing the lipopeptide sodium, the production efficiency of the lipopeptide sodium can be remarkably improved by optimizing a fermentation process, the economic benefit is considerable, and the bacillus subtilis 2KR-18.1.13-26 has relatively high implementation, popularization and application values.

This invention discloses a cyclic lipopeptide delivery system, comprising a cyclic lipopeptide carrier and nucleic acid; the head of the cyclic lipopeptide carrier is composed of heptapeptides R1R2R2R3R4R2R2 linked together by amide bonds to form a ring, and the hydrophobic tail of the cyclic lipopeptide carrier is composed of a fatty acid with a carbon chain length of 6-44 forming an ester bond with the hydroxyl group on the head amino acid R3, with the following general structural formula: where R1 is a basic amino acid, including one of histidine (His), lysine (Lys), or arginine (Arg); R2 is a hydrophobic amino acid, including one of leucine (Leu), isoleucine (Ile), or valine (Val); R3 is a hydrophilic amino acid, including one of tyrosine (Tyr), serine (Ser), or threonine (Thr); R4 is one of glutamate (Glu) or glutamine (Gln); n is an integer from 5 to 43; the cyclic lipopeptide carrier encapsulates the nucleic acid drug to form cyclic lipopeptide-nucleic acid nanoparticles.

This invention discloses a method for preparing a composite spray that improves hemostasis at wound sites. The composite spray includes solution A and solution B, where solution B is an aqueous solution of sodium alginate. The preparation method of solution A includes: 1. Dissolving a lipopeptide conjugate, cholesterol, distearylphosphatidylcholine, distearylphosphatidylserine, and dimethyl sulfoxide in a chloroform-methanol mixed solution to obtain a mixed system; 2. Preparing a precursor solution from the mixed system using a thin-film hydration method; 3. Adding calcium chloride to the precursor solution and reacting it in the dark to obtain solution A. This invention designs the precursor solution based on the function of natural platelets, and its function is equivalent to that of nanoplatelets. Combined with sodium alginate, it can specifically alleviate the adverse effects on the coagulation system caused by blood loss, such as fibrin degradation and reduced platelet activation, at wound sites. It has the characteristics of rapid hemostasis, good bioactivity, and rapid wound healing.

Provided herein are a lipopeptide building block comprising a lipid moiety, an amphipathic domain, a hydrophilic polymer block, a protease cleavage site, and lipopeptide constructs, lipopeptide aggregates and immunogenic compositions comprising the same.