33 results about "Lipopeptide" patented technology

The present invention belongs to the technical field of daily chemical industry, and specifically relates to an emulsion and a preparation method thereof. The emulsion provided by the present invention comprises an emulsifier, glycerol, an oil-phase active substance, pentaerythrityl tetra(ethylhexanoate) and water; wherein the emulsifier is a lipopeptide; the weight percentage of the lipopeptide relative to glycerol is not less than 3%; the weight ratio of the oil-phase active substance to pentaerythrityl tetra(ethylhexanoate) is 1:(3 to 13); the ratio of the total weight of the lipopeptide and glycerol to the total weight of the oil-phase active substance and pentaerythrityl tetra(ethylhexanoate) is 1:(3 to 10); and the ratio of the total weight of the lipopeptide, glycerol, oil-phase active substance and pentaerythrityl tetra(ethylhexanoate) to the weight of water is 1:(1 to 4). By compounding sodium surfactin with glycerol and pentaerythrityl tetra(ethylhexanoate), the emulsion can effectively inhibit the diffusion of the oil-phase active substance at an emulsion interface, reduce the oxidative degradation of the oil-phase active substance, and greatly prolong the effective action time of the oil-phase active substance in the emulsion.

This invention belongs to the field of environmental disinfectant technology, specifically relating to a disinfectant for animal husbandry environments and its preparation method. This invention induces Bacillus subtilis fermentation through a compound extract of hawthorn, codonopsis, and astragalus. The three extracts synergistically upregulate the expression of genes related to antibacterial lipopeptide synthesis, significantly enhancing the antibacterial potency of the fermentation broth. The highly active antibacterial fermentation broth is then incorporated into a sodium alginate-xanthan gum composite gel network. Utilizing the slow oxidation properties of ascorbic acid under environmental humidity and oxygen, the gel network gradually undergoes proton dissociation, achieving a stable and long-lasting release of the antibacterial active ingredients. Simultaneously, xanthan gum swells upon contact with water to form a highly viscoelastic gel film, significantly enhancing the disinfectant's adhesion and retention capacity on humid surfaces. The resulting disinfectant possesses comprehensive properties including high antibacterial activity, long-lasting action, and strong environmental adhesion. The preparation process is simple and controllable, making it suitable for large-scale application in livestock farming environments.

The present invention provides a cosmetic composition comprising alkoxysalicylic acid or a salt thereof, which has a good feel and stability. [Solution] (A) Formula (a): JPEG2026101540000011.jpg4474 Alkoxysalicylic acid or a salt thereof, represented by the formula, (wherein R a (These are alkyl groups with 1 to 6 carbon atoms.) (B) Lipopeptide compounds having a cyclic structure with an alkyl chain having 6 or more carbon atoms, or a salt thereof, (C) Anionic surfactant or amphoteric surfactant A cosmetic product containing [the specified ingredient].

The application provides a kind of lipopeptide compound PW-1 and its preparation method and application in preventing and controlling bacterial wilt, belong to biological pesticide technical field;The PW-1 is prepared by fermentation, solvent extraction, acidification and reverse phase chromatography from Paenibacillus polymyxa (P. Paenibacillus polymyxa ) strain DW-7A;The core of the compound is that it plays a role through "anti-toxicity" mechanism, at the concentration without inhibiting the growth of Pseudomonas solanacearum (P. Ralstonia solanacearum ), can specifically interfere with the biosynthesis of pathogenic bacteria flagellum, cause its motility, chemotaxis and early infection ability to decrease significantly;Pot experiment proves that the relative control efficiency of PW-1 to tobacco bacterial wilt reaches 62.2%, and has no effect on the growth of soil bacillus at the concentration of up to 1000 μg / mL, shows efficient, targeted and environment compatible characteristics;The application provides a new candidate compound and key technical support for the development of green pesticide targeting flagellum.

This invention relates to the field of lipopeptide production technology, and discloses a high-lipopeptide-producing Bacillus subtilis engineered strain, its construction method, and its applications. The engineered strain of this invention is constructed using Bacillus subtilis 168 as the substrate bacterium. The construction method involves: inserting exogenous genes ilvBN and sfp into the substrate bacterium genome; replacing the promoters of endogenous genes bcd, fabHB, BKDAB, lipA, lipM, leuC, and leuA with strong promoters to enhance expression; and knocking out the endogenous gene ilvA. Through the above gene editing, this invention achieves synergistic enhancement of the supply of specific amino acids while maintaining overall cellular metabolic homeostasis, effectively improving the efficiency of NRPS assembly of surfactantin lipopeptides. Fermentation using the above-mentioned Bacillus subtilis engineered strain of this invention can yield high-yield lipopeptides.

The application discloses a kind of lipopeptide class for preventing and treating walnut rot disease and preparation method thereof, it is related to plant disease control technical field, including the nanocarrier core of lipopeptide class loading;And the responsive material shell of the carrier core outside is covered;Wherein, the responsive material shell can be degraded under the microenvironment of walnut tree disease site, the microenvironment characteristics include pH value is lower than 6.0 and there is pectinase activity.The present application realizes the precise and efficient prevention and treatment of walnut rot disease by constructing the intelligent nanometer drug delivery system of "nuclear shell structure", realizes the targeting and on-demand release of lipopeptide in lesion site using the shell with double response to disease microenvironment, greatly improves drug utilization and prolongs the effective period;Nanocarrier core effectively protects lipopeptide activity, and its 50-200 nanometer optimized particle size and surface modification promote drug delivery and enrichment in tree body.

The application of ceramide in the control of rice blast fungus falls within the field of biological control. This invention relates to the application of ceramide in the control of rice blast fungus: a combination of ceramide and lipopeptides is used for the control of rice blast fungus. Exogenous addition of ceramide significantly enhances the stress effect of lipopeptides on rice blast fungus. It also enhances the abnormal accumulation of glycogen and lipid droplets in conidia, promotes the bursting of ROS, and increases intracellular ceramide and calcium... 2+ The invention increases the content of malondialdehyde (MDA) in mycelium, enhances membrane lipid peroxidation, and decreases ergosterol content. Furthermore, it inhibits the expression of ergosterol synthesis genes, UPR target genes, and sphingolipid metabolism-related genes. This invention develops a highly efficient natural agent against rice blast by combining exogenous Cer with lipopeptides. This agent effectively reduces the pathogenicity of rice blast fungus and significantly inhibits its infection of rice, laying the foundation for the development of novel biological control agents.

Provided herein are compounds of Formula (I-A), or salts thereof, including any isomer of the above, wherein the variables are as defined herein. Such compounds can be useful in methods of treating an eosinophilic condition, disease, or disorder. Such compounds can be administered orally. Compositions described herein can be used to reduce eosinophil effector function.

The invention relates to a pest control composition comprising a phenol, such as thymol, an aldehyde, such as vanillin, one or more of the following: a free fatty acid, a fatty acid salt, and a lipopeptide, and a carrier and / or solvent. The invention further relates to a pest control composition comprising thymol, an oil selected from the group consisting of rapeseed oil, soybean oil, and diesel fuel, a stabilizer, and a solvent. The invention also relates to methods for preparing these pest control compositions and to uses of these compositions.

This invention discloses a Bacillus bellis strain overexpressing a lipopeptide synthase gene, its construction method, and its application in fruit preservation. It belongs to the fields of microbial genetic engineering and postharvest fruit preservation technology. Using Bacillus bellis QB250122 as the starting strain, two rounds of genome editing were performed using a dual-plasmid temperature-sensitive CRISPR-Cas9n system. In the first round, the srfA promoter was replaced with the PHpaII superpromoter. In the second round, a complete expression cassette was inserted at the bacA site. The engineered strain produced 900-1200 mg / L of surfactantin. Applying a bacterial suspension to the surface of fruits such as mangoes can extend shelf life by 10-15 days.

This application provides a novel lipopeptide molecule, its preparation method, and its applications. The novel lipopeptide molecule of this application possesses ultra-low interfacial tension, surface tension, and extremely strong wetting reversal properties. Compared with naturally derived lipopeptide compounds, it exhibits stronger stability and higher surface activity, and has broad application prospects in the field of petroleum extraction.

The application discloses a kind of production lipopeptide surfactant and utilize the remediation method of the surfactant to oil hydrocarbon contaminated soil.The preparation method of the surfactant includes: to Bacillus amyloliquefaciens A3 strain is carried out fermentation culture, obtain the fermentation broth containing surfactant;Bacillus amyloliquefaciens A3 strain, preservation number is CGMCC No.6676.The optimal conditions for producing surfactant are: carbon source glycerol, nitrogen source urea, temperature is 25 DEG C, pH is 8.0, carbon source content is 40g / L.The maximum yield of surfactant can be obtained at 150rpm, 72h cultivation is 1.4114g / L.The product of the application has good emulsifying activity and surface activity, and the stability under harsh conditions is also very high, and has very good leaching effect in simulated in-situ remediation soil column leaching experiment compared with control group, and promotes the biodegradation of in-situ soil, and can be used for the remediation of oil hydrocarbon contaminated soil.

This invention belongs to the field of cosmetic raw material technology, and particularly relates to a preservative and antibacterial composition, its preparation method, and its application. The preservative and antibacterial composition comprises the following components in weight percentages: 10-30% antimicrobial lipopeptide, 0.1-1% lysozyme, 0.01-0.05% capryloyl hydroxamic acid, 0.2-0.6% p-hydroxyacetophenone, and the balance being water. This invention screens and extracts natural compounds with antibacterial and preservative activities—antimicrobial lipopeptides and natural antimicrobial substances—lysozyme from natural microbial sources, and combines them with two chemical preservatives, capryloyl hydroxamic acid and p-hydroxyacetophenone, to prepare a novel preservative and antibacterial composition. This compounding method significantly reduces the amount of chemical preservatives used, thereby ensuring the safety and low toxicity of the composition. Furthermore, the components work synergistically to further enhance the preservative and antibacterial properties of the composition.

The present application relates to a kind of oil displacement agent, in particular to a kind of hybrid modified multi-dimensional biological oil displacement agent and its preparation method.The hybrid polymeric biological surfactant of the present application is the product of esterification reaction of biological surfactant and polycarboxylic compound;The biological surfactant is at least one of lipopeptide, sophorolipid, rhamnolipid, sophorolipid modified derivative and rhamnolipid modified derivative;The polycarboxylic compound is at least one of oxalic acid, 1,3-propane dicarboxylic acid, butane tetracarboxylic acid, 1,3-butane dicarboxylic acid and polycarboxylic compound containing saturated alkyl chain with 18 carbon atoms or less between two carboxyl groups.The oil displacement agent provided by the present application has excellent temperature resistance, salt resistance, oil washing efficiency and imbibition oil displacement capacity.In particular, the oil displacement agent can be applied to extreme low permeability reservoir environment below 200 DEG C and below 200 g / L of salinity, and still maintain good chemical stability and interfacial activity under such harsh conditions.

The present application relates to the technical field of pet skin care, and specifically discloses a preparation for preventing or treating pet skin fungal infection and a preparation method thereof, which adopts an aerobic-anaerobic ladder type composite fermentation process, takes coix seed, mugwort leaf and purslane as substrates, inoculates bacillus amyloliquefaciens for aerobic fermentation, utilizes enzyme production of the bacillus amyloliquefaciens to break the wall of the herbal components and enriches lipopeptide substances, then inoculates lactobacillus rhamnosus and propionibacterium freudenreichii for anaerobic fermentation, releases free glycoside bases through mutual metabolism, synthesizes short-chain fatty acids such as propionic acid, and obtains the preparation after centrifugal sterilization. The present application realizes the organic integration of the herbal efficacy and the microecological metabolism, can effectively inhibit pathogenic fungi such as microsporum canis, has multiple effects of anti-inflammatory, antipruritic and repairing pet skin barrier, is safe and mild, and has a stable and controllable process.

This invention discloses a strain of *Bacillus amyloliquefaciens* DY1001 and its applications. This strain was obtained from plant rhizosphere soil through isolation culture and multiple rounds of pathogen antagonism screening. Molecular biological identification combined with morphological observation confirmed its amyloliquefaciens classification, and its microbial preservation number is CGMCC No. 37878. Experimental results show that the secondary metabolites of strain DY1001, such as extracellular crude lipopeptides, have superior antibacterial effects against the pathogens of maize leaf spot and maize scab. Furthermore, it also exhibits inhibitory effects against maize leaf spot, maize stem rot, wheat stem rot, wheat scab, and soybean root rot, demonstrating broad-spectrum antibacterial activity. It shows broad application prospects in plant disease control and provides a high-quality strain resource for the preparation and development of biocontrol agents.

The application provides an antibacterial peptide composition, a preparation method and application, and comprises a composite probiotic core layer, an antibacterial lipopeptide loading layer and a CaCO3 nanocrystal buffered alginate / pectin composite microcapsule coating; the composite probiotic layer comprises a bacteria slurry composed of Ruminococcus lactaris CY12 and S. salivarius 7247 at a ratio of 1:1 in terms of viable bacterial number, and the total viable bacterial number is greater than or equal to 5.0x10 9 CFU / g; and the antibacterial lipopeptide layer comprises SEPs candidate peptides mined from human intestinal metagenome, and the total addition amount is 0.1%-0.5%. The antibacterial peptide composition, the preparation method and the application are used, gastric acid tolerance and colon targeting accuracy better than traditional pH-dependent coating are realized, natural synergy of maintenance and defense is realized, and excellent efficacy can be achieved without genetic engineering modification.

This invention discloses a class of dendritic lipopeptide molecules that promote the oral absorption of macromolecular drugs, their preparation method, and applications. The dendritic lipopeptide molecules are formed by combining a hydrophilic head and a hydrophobic tail. The hydrophilic head consists of lysine and glutamic acid linked by amide bonds, while the hydrophobic tail is composed of organic carbon chains or fluorinated chains. The oral absorption enhancer used in this invention exhibits amphoteric characteristics, possessing excellent anti-protein adsorption capabilities, superior mucus penetration and small intestinal permeability, and good biocompatibility due to its amino acid element construction. The oral absorption enhancer used in this invention can cross multiple barriers, including the mucus layer and intestinal epithelial cells, without opening tight junctions, facilitating efficient and safe oral encapsulation and delivery of proteins.

The present application relates to the technical field of microbial culture and biological control, and particularly relates to a method for inducing high-efficiency synthesis of antibacterial lipopeptide by bacillus, wherein the high-efficiency synthesis of antibacterial lipopeptide is promoted by co-culture induction through always contacting metabolites of pathogenic fungi during the culture of bacillus; the bacillus is bacillus velezensis EM-1, and the preservation number is CGMCC No.21131; the pathogenic fungi is alternaria alternata XN01, and the preservation number is CGMCC No.42504. Through the physical isolation of bacillus velezensis EM-1 and alternaria alternata XN01, pure chemical communication between microorganisms is realized, the synthesis amount of lipopeptide antibacterial substances such as fengycin in bacillus velezensis EM-1 is significantly improved, and a new way for developing efficient and stable microbial biocontrol agents is provided.

The application relates to the technical field of enzymolysis treatment, in particular to an enzyme hydrolysis device for extracting bacteriostatic composite lipopeptide, which comprises a reactor and a top cover, the top cover is fixedly installed at the top of the reactor; a driving assembly is arranged in the interior of the reactor; a stirring assembly is arranged in the interior of the reactor and comprises a connecting assembly and a blade assembly. The inclination directions of the second paddle and the third paddle are adjusted, so that the stirring assembly synchronously pushes the mixed solution downward in the enzyme hydrolysis stage, the mixing uniformity is improved, the first paddle pushes upward, the second paddle and the third paddle push downward in the discharging stage, rapid discharging is realized, the residues in the tank are reduced, vortex gas entrainment and foam scum are inhibited, the reciprocating movement of the rotating piece three is used, so that in the enzyme hydrolysis stage, the stirring action is concentrated in the lower half area of the reactor, the bottom material disturbance is intensified, the substrate settlement and deposition are effectively inhibited, in the discharging stage, the upper liquid surface area is disturbed in a targeted manner, the scum and foam are broken, and the material blockage is avoided.

This disclosure relates to a method for improving a fermentation process for producing antifungal lipopeptides and itursin by supplementing the fermentation medium with glutamate using Bacillus amyloliquefaciens.

The present application belongs to the technical field of feed additive, and particularly relates to a feed additive for increasing the content of branched-chain fatty acids in milk and a preparation method thereof.The feed additive for increasing the content of branched-chain fatty acids in milk comprises the following raw materials in parts by weight: 1-4 parts of leucine, 1-4 parts of isoleucine, 1-4 parts of valine, 1-2.5 parts of Prinsepia uniflora extract and 0.3-0.6 parts of sodium subtilisin lipopeptide.The feed additive for increasing the content of branched-chain fatty acids in milk is prepared by using different types of amino acids and Prinsepia uniflora extract and sodium subtilisin lipopeptide in combination, and can significantly increase the content of branched-chain fatty acids in milk, improve the quality of milk and the milk production performance of dairy cows after being used in the daily ration of dairy cows.The feed additive is safe in composition and has no toxic side effects.

The present application belongs to the field of daily chemical products, and particularly relates to a full-biological private care gel composition and a preparation method thereof. The composition comprises a biosugar lipid active substance, a lipopeptide and a plant saponin. The formula of the present application is experimentally proved to have the functions of inhibiting Staphylococcus aureus, Escherichia coli and Aspergillus niger, and removing the biofilm formed by Staphylococcus aureus and Candida albicans.

The invention relates to the technical field of oilfield chemistry, in particular to bacillus subtilis zymophyte liquid, a microbial composite nano bactericide and a preparation method thereof, and the preparation method of the bacillus subtilis zymophyte liquid comprises the following steps: culturing a bacillus subtilis strain in a culture medium to obtain the bacillus subtilis zymophyte liquid; wherein the culture medium is a fermentation culture medium or a hydrocarbon degradation culture medium. Fermentation bacteria liquid of the bacillus subtilis strain contains lipopeptide biosurfactants, can play roles of dispersing agents, stabilizing agents, blocking agents, reducing agents and the like in metal nanometer synthesis, can inhibit excessive growth of nanometer particles and prevent the nanometer particles from being gathered / condensed in colloid synthesis, and has the capacity of inducing tiny changes of the nanometer particles. The microbial composite nano bactericide is prepared on the basis of the zymophyte liquid, AgNPs in the microbial composite nano bactericide is small in particle size and good in dispersity and stability, and harmful florae such as sulfate reducing bacteria in the oil field environment can be well inhibited.

The present application provides an anti-aging plant composition and a preparation method thereof, and belongs to the field of medical, dental or cosmetic formulations. The composition is compounded by Pseudomonas stutzeri fermented Coptis extract, Bacillus subtilis fermented Dendrobium officinale extract, Galangal extract and plant oil rich in polyunsaturated fatty acids. The lipopeptide biosurfactant produced in situ by directional fermentation of double bacteria is combined with high shear homogenization process to realize sub-micron self-emulsification of oil without adding chemical surfactants. The present application significantly improves the transdermal penetration of active components and the synergistic anti-aging effect, and has a refreshing skin feel and high safety, and is suitable for various anti-aging cosmetics.

This invention provides a full-aqueous phase extraction process for sodium lipopeptides based on molecular weight sieving and pH control coupling. The process includes the following steps: The fermentation broth is heated to denature proteins and co-precipitate them with bacterial cells; the precipitate is removed by high-speed centrifugation; the precipitate is washed with acidic brine as an "anti-solvent" to enhance impurity removal; an optimal solution pH is established before ultrafiltration using an alkaline aqueous solution to precisely retain the target lipopeptides and remove proteins, polysaccharides, etc. from the solution; after adjusting the solution pH to neutral, deep desalination and concentration are simultaneously completed using nanofiltration constant-volume washing technology; solid sodium lipopeptides are obtained by spray drying. This invention utilizes a multi-stage membrane filtration system of "microfiltration-ultrafiltration-nanofiltration" to achieve step-by-step, targeted removal of impurities; extraction is performed in a full-aqueous phase, eliminating organic solvents and avoiding organic solvent residues throughout the production process, making it more environmentally friendly and safer; the final product is sodium lipopeptide salt, improving the product's water solubility and stability, and preserving the product's bioactive structure to the greatest extent.

The present invention addresses the problem of providing: a new antigen that can also be effective against mutant viruses; and a new vaccine obtained by using said antigen. Said problem is solved by providing a lipopeptide which is obtained by conjugating a lipid and a peptide and in which the peptide has antigenicity and is represented by formula (1): a-b-c-d(-e-f)n. In the formula, n represents 0 or 1, and a-f include a specific amino acid.

The application provides a bacillus coagulans (Bacillus coagulans) Bacillus coagulans ) and application thereof. The bacillus coagulans of the application is preserved in the China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No. 36786. The bacillus coagulans of the application has multiple antimicrobial lipopeptide genes and ACC deaminase genes; has strong stress resistance, high fermentation activity, and the ability to produce antimicrobial lipopeptides and secrete plant growth hormones; can effectively prevent and control various crop diseases, and can also directly promote the growth and development of crops, improve the stress resistance of crops, and promote the yield increase of crops.

This invention provides a method for synthesizing adenosine-containing compositions via microbial fermentation, as well as the adenosine-containing compositions. By introducing a trace amount of Bacillus subtilis, which is capable of producing subtilis lipopeptides, into the later stages of fermentation, which is prone to problems such as low dissolved oxygen and low glycoside production, the subtilis lipopeptides produced by Bacillus subtilis act as a super biosurfactant, continuously emulsifying the culture medium and significantly reducing the surface tension of the culture medium. This prevents the crystallization of high concentrations of adenosine in the culture medium and increases its dissolved oxygen level, thus greatly improving the yield of adenosine. Furthermore, the resulting compositions can be directly used as cosmetic raw materials.