172 results about "Lipopeptide" patented technology

The application belongs to the technical field of daily chemical industry, and particularly relates to an emulsion and a preparation method thereof. The emulsion provided in the application comprises an emulsifier, glycerol, oil-phase active substance, pentaerythritol tetra(ethylhexanoate) and water; the emulsifier is a lipopeptide; the weight percentage of the lipopeptide and the glycerol is not less than 3%; the weight ratio of the oil-phase active substance and the pentaerythritol tetra(ethylhexanoate) is 1:3-10; the ratio of the sum of the weights of the lipopeptide and the glycerol to the sum of the weights of the oil-phase active substance and the pentaerythritol tetra(ethylhexanoate) is 1:3-10; and the ratio of the sum of the weights of the lipopeptide, the glycerol, the oil-phase active substance and the pentaerythritol tetra(ethylhexanoate) to the weight of the water is 1:1-4. The emulsion is prepared by using sodium bacillomycin, glycerol and pentaerythritol tetra(ethylhexanoate), can effectively inhibit the diffusion of the oil-phase active substance at the interface of the emulsion, reduce the oxidative degradation of the oil-phase active substance, and greatly prolong the effective action time of the oil-phase active substance in the emulsion.

In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, the disclosure, in one aspect, relates to lipid nanoparticles for use as drug delivery agents. The lipid nanoparticles are composed of lipopeptide conjugates, where a peptide is bonded to a lipid. The lipid nanoparticles described herein represent a new approach in drug delivery, addressing critical challenges in balancing biodegradability, biocompatibility, and organspecific targeting. The lipid nanoparticles can selectively target and deliver bioactive agents to specific tissues in a subject by modifying specific amino acids and ratios thereof in the peptide of the lipopeptide conjugate.

The invention relates to bacillus for producing lipopeptide, bacteriostatic lipopeptide of the bacillus and application of the bacillus to aquatic products, and belongs to the technical field of microorganisms. The bacillus velezensis is a bacillus velezensis strain FJAT-57093, is preserved in the China General Microbiological Culture Collection Center on April 1, 2025, and has a preservation number of CGMCC No. 34063, and the bacillus velezensis strain FJAT-57093 is a bacillus velezensis strain FJAT-57093, is preserved in the China General Microbiological Culture Collection Center on April 1, 2025, and has a preservation number of CGMCC No. 34063. The bacteriostatic lipopeptide is prepared from three types of lipopeptides, namely, iturin, fengycin and surfactin. The invention further discloses a preparation method of the bacteriostatic lipopeptide. The bacillus and the bacteriostatic lipopeptide have a broad-spectrum bacteriostatic effect on aquatic pathogenic bacteria, can be used for aquaculture, and have a wide application prospect.

The invention relates to the technical field of microorganisms, in particular to bacillus velezensis NEAU-L05, application of the bacillus velezensis NEAU-L05, antibacterial cyclic lipopeptide Iturin A and application of the antibacterial cyclic lipopeptide Iturin A. The bacillus velezensis NEAU-L05 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center, the address is Institute of Microbiology, Chinese Academy of Sciences, 3 #, 1 # yard, West Beichen Road, Chaoyang District, Beijing, the preservation date is December 19, 2024, and the preservation number is CGMCC No.33130. The bacillus velezensis NEAU-L05 disclosed by the invention can be used for preparing the bacillus velezensis NEAU-L05. The bacteriostasis rate of the bacterial strain to botrytis cinerea of phytopathogen is 67.2%. The bacteriostatic cyclic lipopeptide ItuinA separated from the strain can improve the storage performance of berries, and a basis is provided for development of multiple biocontrol microbial agents with excellent performance.

Described are lipopeptides that inhibit coronavirus fusion to a host cell. Thus a therapeutic for treating or preventing the common cold is described along with methods of inhibiting and / or treating an alphacoronavirus infection. The lipopeptides comprise a peptide unit comprising an amino acid sequence having a high degree of sequence identity to a sequence from the C-terminal heptad repeat of a betacoronavirus S protein, such as that of SARS-CoV-2.

The invention discloses a bacterial strain C5A-1 capable of producing lipopeptide and having a plant growth promoting function and application of the bacterial strain C5A-1, solves the technical problem of promoting plant growth, especially root length, plant height, stem diameter, leaf number, leaf area, underground dry weight and overground dry weight, and particularly discloses bacillus velezensis C5A-1 or a variant or a progeny thereof. The bacillus velezensis C5A-1 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.32880. When the bacteria or the fermented supernate thereof or the combination thereof is applied to plants, the growth of the plants can be promoted, and the bacteria can be used for industrial production.

The invention relates to a method for improving fermentation production efficiency of bacillus subtilis lipopeptide sodium by controlling threonine flow acceleration rate based on fermentation process oxygen uptake rate curve feedback. Bacillus subtilis is used as a production strain, and lipopeptide sodium is produced through deep liquid state fermentation. In the fermentation process, the oxygen uptake rate of the process is detected, a threonine and ammonium sulfate continuous feeding control process based on oxygen uptake rate curve parameters is implemented, the oxygen uptake rate in the fermentation process is stably maintained to be 60-65 mmo1 / L / h, the ammonium ion concentration is maintained to be 15-20 mmol / L, and rapid growth of hyphae and rapid increase of the lipopeptide sodium synthesis rate can be achieved. The threonine and ammonium sulfate fed-batch control process based on oxygen uptake rate feedback control is established, the production period is shortened, and the fermentation production level is stably improved.

The invention discloses recombinant bacillus subtilis with high yield of surfactin as well as a construction method and application of the recombinant bacillus subtilis, and belongs to the technical field of genetic engineering. According to the invention, non-essential genes are determined through analysis and identification, a simplified engineering strain is obtained, and the highest fermentation yield reaches 11.2 g / L; then, a PTS part sugar phosphorylase gene obtained through screening is transferred into the simplified engineering strain, the metabolism efficiency of carbohydrates is improved, transportation and utilization of the carbohydrates are optimized, a fatty acid transmembrane transporter gene is further transferred, fatty acid uptake is assisted, synthesis of surfactin is facilitated, and the yield of the surfactin is improved. The combination of the two realizes optimization of synthesis of surfactin through a composite carbon source. Compared with a simplified engineering strain, the genetically engineered bacterium obtained by the invention has the advantages that the yield of surfactin is increased by 57.1%, the yield is increased by 2.3 times compared with that of an unsimplified engineering strain EX, and the final shake-flask fermentation yield can reach 17.6 g / L. As an industrial chassis cell of high-yield lipopeptide, the cell has a good industrial application prospect.

The invention provides a high-temperature-resistant lipopeptide reinforced foam flooding system and a screening method and application, and relates to the technical field of microbial oil recovery, the high-temperature-resistant lipopeptide reinforced foam flooding system comprises the following steps: S1, screening applied oil reservoirs; s2, based on the oil reservoir temperature and the crude oil viscosity selected in S1, screening a high-temperature-resistant lipopeptide reinforced foam flooding system; the high-temperature-resistant lipopeptide reinforced foam oil displacement system comprises a foaming agent, a foam stabilizer, gas and water, the foaming agent comprises one or more of surfactin (C12-18), scabrin (C14-18), iturin (C14-17), deocytin, serratia marcescens and mucin fermentation liquor, and the foaming agent comprises one or more of lipopeptide, a foaming agent and a foaming agent. The foam stabilizer comprises one or more of biosynthetic nano-silver, nano-iron, nano-copper, nano-zinc, nano-zirconium and nano-nickel. The method has the beneficial effects that the oil reservoir application range is wide, the pertinence is high, the field test effect is good, and a high-temperature-resistant lipopeptide enhanced foam oil displacement system is specifically constructed according to the temperature-resistant stability characteristics of lipopeptide surfactants and biosynthetic nano materials of different structures and types.

The present invention discloses a cyclic lipopeptide carrier for encapsulating nucleic acid drugs, comprising a cyclic lactone structure formed by a heptapeptide R1R2R2R3R1R2R2 and a β-hydroxy fatty acid with a carbon chain length of 6 to 44, and its general structural formula is as follows: #imgabs0# wherein R1 is a basic amino acid, including one of histidine His, lysine Lys or arginine Arg, R2 is one of the hydrophobic amino acids leucine Leu, isoleucine Ile or valine Val, and R3 is one of any amino acid; n is an integer of 2 to 40; the positively charged basic amino acids in the cyclic lipopeptide bind to the negatively charged nucleic acid drug through electrostatic interaction to form cyclic lipopeptide-nucleic acid particles. The cyclic lipopeptide carrier for encapsulating nucleic acid drugs provided by the present invention has high safety, can effectively protect nucleic acid drugs, improves their stability, and has good transfection efficiency for a variety of cells, and has broad application prospects in the field of nucleic acid drug delivery.

This application discloses a thickening composition and an amino acid surfactant, including a lipopeptide biosurfactant. The lipopeptide biosurfactant possesses the basic cleaning and foaming properties of surfactants while thickening the amino acid surfactant and its compound system, synergistically improving cleaning ability without affecting the system's appearance or transparency. The lipopeptides in this application are produced by bacteria, are a green source of raw materials, are mild and non-irritating, harmless to humans and environmentally friendly, biodegradable, and pose no harm with long-term use.

The present invention relates to a cyclic peptide, a conjugate comprising said cyclic peptide and a lipopeptide building block, a bundle of said conjugates, a synthetic virus-like particle comprising at least one bundle of conjugates and pharmaceutical compositions comprising the same. The present invention further relates to said cyclic peptide, said conjugate said bundle of conjugates, said synthetic virus-like particle and said pharmaceutical compositions for use as a medicament, preferably for use in a method for preventing of an infectious disease or reducing the risk of an infectious disease, more preferably for use in a method for preventing or reducing the risk of an infectious disease associated with or caused by a respiratory syncytial virus.

The invention belongs to the technical field of biological extracts and cosmetics, and discloses a leukocyte extract composition as well as a preparation method and application thereof. The leukocyte extract composition comprises the following components in percentage by mass: 5-20% of a leukocyte extract; 3-10% of sodium surfactin; 10-20% of a humectant; and 50-82% of water. The preparation method of the leukocyte extract composition comprises the following preparation steps: adding the sodium surfactin into hot water at 50-80 DEG C for dissolving, then adding the humectant, homogenizing, stirring and uniformly mixing, cooling to room temperature, then adding the leukocyte extract, stirring and uniformly dissolving to obtain the leukocyte extract composition. According to the composition, a specific cyclic lipopeptide compound, namely, sodium surfactin, is adopted as a synergistic stable synergist of the leukocyte extract, so that the activity stability of the leukocyte extract can be remarkably improved, and better and more stable anti-aging, repairing and whitening effects can be achieved under the condition of smaller addition amount.

The present invention belongs to the technical field of daily chemical industry, and specifically relates to an emulsion and a preparation method thereof. The emulsion provided by the present invention comprises an emulsifier, glycerol, an oil-phase active substance, pentaerythrityl tetra(ethylhexanoate) and water; wherein the emulsifier is a lipopeptide; the weight percentage of the lipopeptide relative to glycerol is not less than 3%; the weight ratio of the oil-phase active substance to pentaerythrityl tetra(ethylhexanoate) is 1:(3 to 13); the ratio of the total weight of the lipopeptide and glycerol to the total weight of the oil-phase active substance and pentaerythrityl tetra(ethylhexanoate) is 1:(3 to 10); and the ratio of the total weight of the lipopeptide, glycerol, oil-phase active substance and pentaerythrityl tetra(ethylhexanoate) to the weight of water is 1:(1 to 4). By compounding sodium surfactin with glycerol and pentaerythrityl tetra(ethylhexanoate), the emulsion can effectively inhibit the diffusion of the oil-phase active substance at an emulsion interface, reduce the oxidative degradation of the oil-phase active substance, and greatly prolong the effective action time of the oil-phase active substance in the emulsion.

The application discloses a floating composite microbial preparation for removing marine oil pollution and a preparation method thereof. The floating composite microbial preparation contains bacillus thuringiensis NH26 strain, hollow glass microbeads and a lipopeptide surfactant purified from a fermentation liquor of the NH26 strain, has floating characteristics, can rapidly activate and efficiently degrade oil hydrocarbon, has good degradation capacity for alkanes and polycyclic aromatic hydrocarbons in seawater, and still has good degradation capacity for alkanes in seawater containing high-concentration oil hydrocarbon. The floating composite microbial preparation can be used for removing marine oil pollution and repairing marine environment. In addition, the application further provides a preparation method of the floating composite microbial preparation. The preparation method has simple preparation process, the obtained floating composite microbial preparation has uniform particle size, has high embedding rates for nitrogen and phosphorus, and can be prepared on a large scale.

The invention provides a composite quality control product of an AIDS antibody, a syphilis antibody and a hepatitis C antibody as well as a preparation method and application of the composite quality control product. The composite quality control product comprises an AIDS (Acquired Immune Deficiency Syndrome) antibody, a syphilis antibody, a hepatitis C antibody and the following components: a matrix solution, 1.5 to 3 g / L of Pluronic F68, 0.05 to 0.15% v / v of ProClin 300, 0.1 to 1 g / L of sodium surfactin, 1 to 10 g / L of bovine serum albumin, 1 g / L of alanine, 2 to 5 g / L of trehalose, 0.5 to 1.0 g / L of EDTA (Ethylene Diamine Tetraacetic Acid) and 2 to 5 mmol / L of benzamidine. The matrix liquid comprises a buffer solution and 20-30% v / v human serum. According to the invention, the aggregation and inactivation risk of the antibody in the freezing and thawing process is effectively reduced, the antibody can be repeatedly frozen and thawed for more than three times, the degradation of the antibody at high temperature can be effectively prevented, and the reliability of the quality control product in the transportation and clinical use process is ensured.

The invention discloses bacillus subtilis for producing lipopeptide sodium and application of the bacillus subtilis, and belongs to the technical field of microbial engineering. The preservation number of the bacillus subtilis is CGMCC (China General Microbiological Culture Collection Center) No.34998 The strain is only subjected to basic fermentation, the yield of the lipopeptide sodium can reach 19.3 g / L, four lipopeptide sodium analogues can be synthesized in the fermentation process, the abundance of products is improved, and meanwhile, a new biological resource is provided for industrial and efficient production of the lipopeptide sodium. When the bacillus subtilis 2KR-18.1.13-26 provided by the invention is used for producing the lipopeptide sodium, the production efficiency of the lipopeptide sodium can be remarkably improved by optimizing a fermentation process, the economic benefit is considerable, and the bacillus subtilis 2KR-18.1.13-26 has relatively high implementation, popularization and application values.

This invention discloses a cyclic lipopeptide delivery system, comprising a cyclic lipopeptide carrier and nucleic acid; the head of the cyclic lipopeptide carrier is composed of heptapeptides R1R2R2R3R4R2R2 linked together by amide bonds to form a ring, and the hydrophobic tail of the cyclic lipopeptide carrier is composed of a fatty acid with a carbon chain length of 6-44 forming an ester bond with the hydroxyl group on the head amino acid R3, with the following general structural formula: where R1 is a basic amino acid, including one of histidine (His), lysine (Lys), or arginine (Arg); R2 is a hydrophobic amino acid, including one of leucine (Leu), isoleucine (Ile), or valine (Val); R3 is a hydrophilic amino acid, including one of tyrosine (Tyr), serine (Ser), or threonine (Thr); R4 is one of glutamate (Glu) or glutamine (Gln); n is an integer from 5 to 43; the cyclic lipopeptide carrier encapsulates the nucleic acid drug to form cyclic lipopeptide-nucleic acid nanoparticles.

This invention discloses a method for preparing a composite spray that improves hemostasis at wound sites. The composite spray includes solution A and solution B, where solution B is an aqueous solution of sodium alginate. The preparation method of solution A includes: 1. Dissolving a lipopeptide conjugate, cholesterol, distearylphosphatidylcholine, distearylphosphatidylserine, and dimethyl sulfoxide in a chloroform-methanol mixed solution to obtain a mixed system; 2. Preparing a precursor solution from the mixed system using a thin-film hydration method; 3. Adding calcium chloride to the precursor solution and reacting it in the dark to obtain solution A. This invention designs the precursor solution based on the function of natural platelets, and its function is equivalent to that of nanoplatelets. Combined with sodium alginate, it can specifically alleviate the adverse effects on the coagulation system caused by blood loss, such as fibrin degradation and reduced platelet activation, at wound sites. It has the characteristics of rapid hemostasis, good bioactivity, and rapid wound healing.

Provided herein are a lipopeptide building block comprising a lipid moiety, an amphipathic domain, a hydrophilic polymer block, a protease cleavage site, and lipopeptide constructs, lipopeptide aggregates and immunogenic compositions comprising the same.

The invention relates to a binary composite oil displacement system containing a bio-based surfactant and a biological surfactant and application of the binary composite oil displacement system. The binary composite oil displacement system comprises the bio-based surfactant and the biological surfactant, the bio-based surfactant is any one of a methylphenyl octadecanamide ethyl hydroxypropyl sulfonic acid quaternary ammonium salt, a benzene methoxy octadecanamide propyl amine hydroxy propanesulfonic acid quaternary ammonium salt, a phenyl octadecanamide propyl amine hydroxy propanesulfonic acid quaternary ammonium salt or a phenyl octadecanamide propyl carboxylic acid quaternary ammonium salt; the biosurfactant is a bacillus subtilis lipopeptide fermentation broth or a lipopeptide extract obtained from the lipopeptide fermentation broth. Compared with a traditional chemical surfactant oil displacement system, the oil displacement system has the advantages of reproducibility, simplicity in preparation and the like, has good temperature resistance, NaCl resistance and Ca < 2 + > resistance, is suitable for high-temperature and hypersalinity oil reservoirs, and has great application potential in tertiary oil recovery.

The invention relates to a complex microbial inoculant and application thereof in prevention and control of tobacco black shank and tomato spotted wilt, and belongs to the technical field of prevention and control of tobacco diseases. The complex microbial inoculant comprises antibacterial lipopeptide and bacillus subtilis, wherein the mass ratio of the antibacterial lipopeptide to the bacillus subtilis is (0.5-1.2): 10; 0.08 wt% to 0.12 wt% of antibacterial lipopeptide powder is adopted as the antibacterial lipopeptide; the antibacterial lipopeptide comprises at least one of surfactin, iturin and abinogen; the viable count of the bacillus subtilis is greater than or equal to 2 * 10 < 9 > CFU / g. According to the invention, a combined prevention and control method taking antibacterial lipopeptide and bacillus subtilis as cores is adopted, so that the synergistic prevention and control of tobacco black shank and tomato spotted wilt are realized, and meanwhile, the agricultural and economic characters of tobacco are improved; due to the dual effects of the bacillus subtilis and the antibacterial lipopeptide, the lasting period can be prolonged, and the stability is high; the complex microbial inoculant can be completely biodegraded and is suitable for organic agriculture.

The invention discloses a method for preparing egg yolk lipopeptide-curcumin composite nanoparticles, comprising the steps of: hydrolyzing fresh egg yolk with protease, centrifuging and taking the supernatant for spray drying, and extracting egg yolk lipopeptide using supercritical carbon dioxide; then adding the egg yolk lipopeptide to deionized water and stirring to obtain an egg yolk lipopeptide solution, dissolving curcumin powder in a NaOH solution, mixing the alkaline curcumin solution with the egg yolk lipopeptide solution, stirring at room temperature in the dark, adjusting the pH value of the mixed solution using an HCl solution, centrifuging the sample solution, taking the supernatant and freeze-drying, and obtaining egg yolk lipopeptide-curcumin composite nanoparticles. The prepared egg yolk lipopeptide-curcumin composite nanoparticles are more stable and have higher bioavailability than free curcumin.

The invention belongs to the technical field of cosmetics, and particularly relates to a shea butter composition as well as a preparation method and application thereof. The shea butter composition is prepared from the following raw materials in percentage by weight: 0.1 to 35 percent of shea butter, 0.01 to 0.5 percent of ceramide, 0.01 to 0.3 percent of sodium bacillus subtilis lipopeptide, 10 to 50 percent of glycerol and the balance of water. The shea butter composition has the beneficial effects that the shea butter composition has better moisturizing and repairing effects on skin, a surfactant used in the preparation process of the shea butter composition is far lower than that used in a traditional emulsion, and the shea butter composition has better popularization advantages; when applied to cosmetics, the product hardly promotes transdermal absorption of harmful substances such as preservatives, and is especially suitable for pregnant women, infants, people with sensitive skin and the like.

This invention belongs to the field of biomedical technology and relates to a drug-loaded vesicle for promoting the healing of diabetic wounds. The drug-loaded vesicle has a core-shell structure. The core contains siRNA targeting ALKBH5 mRNA; the shell is a hybrid membrane of human umbilical vein endothelial cell membrane and milk-derived extracellular vesicle membrane, with its surface embedded with arginine-rich lipopeptides, and at least some arginine exposed outside the hybrid membrane. The core-shell structure effectively protects the siRNA from degradation and reduces off-target interference. The hybrid membrane shell has good biocompatibility, immunogenicity, and low cytotoxicity. The exposed arginine enhances tissue penetration and cellular uptake. In vitro and in vivo experiments have confirmed that this drug-loaded vesicle has good cellular uptake efficiency, effectively downregulates ALKBH5 expression, and restores mRNA levels. 6 A-modification increases the stability of C2orf69 mRNA, improves endothelial cell function, significantly promotes the healing of diabetic wounds, and has good biocompatibility and safety.

The invention provides a composition for preventing and treating plant diseases and application thereof. The composition comprises antibacterial lipopeptide and a protein plant immunity inducer. Synchronous and efficient prevention and control of fungal black shank and viral PVY and CMV diseases are achieved for the first time, the prevention effect reaches 88% or above, and the industrial problem that the prevention and control spectrum of a single agent is narrow is solved.

The present invention relates to the use of apabicin in a method for treating bacterial infections associated with biofilms containing Staphylococcus bacteria, wherein the method comprises administering apabicin in combination with daptomycin and / or one or more additional agents selected from lipopeptides, glycopeptides, and lincosamides, or vancomycin to the patient.

The present invention provides a method for increasing the fermentation yield of Bacillomycin D, using Bacillus subtilis fmbJ as the starting strain and adding the growth factor VC to increase the yield of Bacillomycin D. In the method of the present invention, adding VC to the fermentation medium can increase the relative expression of the Bacillomycin D synthase gene, promote the transcription of key enzyme genes in the amino acid precursor synthesis pathway, and promote degQ 、 comA 、 comP 、 comQ 、 sigM and spo0A The expression of positive regulatory signal factors involved in the synthesis of secondary metabolites was upregulated, while some negative regulatory factors such as rapC and April , which increased the yield of Bacillomycin D to 1.44 times that of the control group; at the same time, the method of the present invention has low cost, simple and convenient operation, can effectively reduce the production cost of lipopeptides, improve production efficiency, and is expected to be used for large-scale production applications.

The invention relates to the technical field of microbial fermentation, and discloses bacillus subtilis, application of the bacillus subtilis in fermented soybean meal and a preparation method of the fermented soybean meal. The bacillus subtilis screened by the invention has strong anti-nutritional factor resistance (such as glycinin, beta-conglycinin, raffinose and stachyose), has a good growth effect in the soybean meal raw material, and is used for fermenting the soybean meal raw material; the content of anti-nutritional factors (such as glycinin, beta-conglycinin, raffinose and stachyose) in the soybean meal raw material can be obviously reduced, and meanwhile, the content of lipopeptide substances (such as surfactin and bacitracin D) in the fermented soybean meal product can be obviously improved; the prepared fermented soybean meal product has a good antibacterial effect on three common intestinal pathogens (escherichia coli, salmonella and staphylococcus) in animal bodies.

The invention discloses a bacillus amyloliquefaciens Jcc3 and application thereof in prevention and treatment of aquatic diseases, the classification name of the bacillus amyloliquefaciens Jcc3 is bacillus amyloliquefaciens, the bacillus amyloliquefaciens Jcc3 is preserved in China Center for Type Microbiological Culture Collection, the preservation time is January 25, 2024, and the preservation number is CCTCC NO: M 2024210. The bacillus amyloliquefaciens Jcc3 disclosed by the invention is identified as a gram positive bacterium. The strain can produce lipopeptides, proteins and other substances with bacteriostatic action, and has a significant inhibitory effect on aquatic disease aeromonas veronii. By inhibiting the growth and reproduction of the aeromonas veronii, aquatic diseases caused by the aeromonas veronii can be effectively prevented and controlled, so that the immunity of aquatic animals is enhanced, and the death rate of the aquatic animals is reduced. In conclusion, the bacillus amyloliquefaciens Jcc3 has great application potential in prevention and control of aquatic diseases, especially in prevention and control of diseases caused by aeromonas veronii.