210 results about "Aeromonas veronii" patented technology

The invention relates to a novel antibiotic and a nucleotide sequence, a preparation method and an application thereof, belonging to the biopharmaceutical field. The novel antibiotic comprises colicinallosteric polypeptide and staphylococcus pheromone peptide chain which are capable of specifically killing pathogenic bacteria combined by the specificity of the antibiotic without damaging the normal cells of human body and are linearly linked with each other to generate novel antibiotics resisting staphylococcus aureus, staphylococcus epidermidis and pseudomonas aeruginosa, has the antisepticeffect as thousand times as the conventional antibiotics, difficult generation of drug resistance, and safer use through overcoming the shortcoming of causing the hypersensitivity of human body of wild colicin.

The invention discloses a bacillus amyloliquefaciens strain as well as a preparation method and application of a strain powder preparation of the bacillus amyloliquefaciens strain, and belongs to the technical field of microorganisms. The bacillus amyloliquefaciens JSSW-LA, which is obtained through screening, is preserved in China Center for Type Culture Collection with preservation number of CCTCC NO: M2015602. The strain powder preparation of the bacillus amyloliquefaciens, which is prepared through freeze-dried culture activation, fermentation culture and strain powder preparation, is applicable to an additive of fishery breeding feed or a water quality improver of aquaculture; the bacillus amyloliquefaciens JSSW-LA provided by the invention is capable of effectively inhibiting the growth of A.sobria, A.hydrophila, A.caviae, aeromonas veronii and edwardsiella tarda, and an inhibitory effect on the A.sobria, A.hydrophila and A.caviae is more excellent than that on other pathogenic bacteria. Therefore, the JSSW-LA strain powder, applied to aquaculture, is capable of effectively preventing and controlling diseases in aquaculture animals caused by pathogenic bacteria, in particular bacterial bleeding disease, so as to protect the healthy growth of aquatic animals.

The invention relates to a separating and screening method for a lytic aquatic pathogenic bacterium phage. The name of the phage is deltaA8-29, the systematic name is Aeromonas veronii phage, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.9152; the name of Aeromonas veronii is A8-29, the systematic name is Aeromonas veronii phage, the preservation number is CGMCC No.9093, the preservation unit is CGMCC, the address is #3, yard 1, West Beichen Road, Chaoyang District, Beijing, and the preservation date is April 25, 2014. The invention provides the screening and identification method of the aquatic pathogenic bacterium phage. The separated phage can be used for preventing and controlling infection of aquatic pathogenic bacteria and avoiding or reducing use of antibiotics in aquaculture so as to develop the green mode of aquaculture.

The present invention is red sea bream antibacterial peptide gene and recombinant yeast expression vector and their preparation process. Through library screening and EST sequence analysis to clone red sea bream antibacterial peptide (hepcidin) gene, recombining the gene to yeast expression vector pPICZalphaA to constitute antibacterial peptide gene expression plasmid pPICZ-rsbHEPC1, and transforming Pichia yeast, yeast strain with stable expression of red sea bream antibacterial peptide is obtained. Bacterium growth inhibiting experiment shows that the recombinant antibacterial peptide has dose dependent inhibiting activity on colibacillus, bacillus pyocyaneus and other two kinds of bacteria. The present invention is suitable for cloning various fish antibacterial peptide gene and expression vector constitution, and the produced antibacterial peptide is used as feed additive for the disease prevention and treatment of fishes and other aquatic animal.

The invention relates to a lactobacillus pentosus strain and a fermentation product of the same. The preservation number of the lactobacillus pentosus LPEM818 in the invention is CGMCC No. 4583 in the China General Microbiological Culture Collection Center. The fermentation product of the lactobacillus pentosus in the invention can inhibit common pathogenic escherichia coli, pseudomonas aeruginosa and staphylococcus aureus in food, and particularly has a strong inhibiting effect on listeria monocytogenes which can cause serious food poisoning. In addition, the metabolite of the strain also can inhibit the growth of aspergillus flavus and cotton and tomato blight pathogens. Consequently, the lactobacillus pentosus strain and the fermentation product thereof play an active role in the fields of food preservation and plant protection.

The invention provides a pharmaceutical composition for inhibiting bacteria and a preparation method thereof, and the pharmaceutical composition is prepared from the following pharmaceutical raw materials in parts by weight: 1 part of Chinese gall, 1-5 parts of garden burnet root, 4-12 parts of humifuse euphorbia herb and 1-8 parts of fructus forsythiae. The composition can effectively inhibit pathogenic aeromonas veronii and be further used for preventing and treating bacterial diseases of fish caused by the bacteria. The invention further provides an application of the pharmaceutical composition in the preparation of drug for inhibiting the aeromonas veronii.

The present invention provides a kind of Chinese medicine composition for treating avian flu and belongs to the field of avian disease treating Chinese medicine composition. The Chinese medicine composition is prepared with honeysuckle powder, forsythia powder, notopterygium powder, balloonflower root powder, red peony root powder and other Chinese medicinal materials in certain proportion. It has high and fast curative effect on avian flu, no medicine residue and no resistance. It is used in preventing and treating avian flu, and may be also used in treating other avian diseases caused by various kinds of pathogens.

The invention discloses a plant composition for an air purifying agent. The plant composition is characterized by consisting of aloe, bracket-plant, sansevieria, orchis cyclochila, monstera deliciosa, hedera helix, cycas, chrysanthemum, kumquat, pomegranate, sun plant, China rose, camellia, aglaia odorata, daisy, calyx canthus, marigold, orchid, sweet osmanthus, caladium bicolor, excoecaria cochinchinensis, lichen, seaweed, rose, violet, jasmine, lemon, rosebush, crape myrtle, carnation, lily of the valley, cactus, sansevieria trifasciata, agave, opuntia basilaris, laciniate kalanchoe herb, stonecrop, bryophyllum and cultivated pineapple. The product can imitate the principle of natural forest plant purification, quickly decompose various toxic and harmful gases indoors through strong chelate reaction and high-order complexing action, can kill colon bacillus, pseudomonas aeruginosa, staphylococcus aureus and the like with sterilizing rate reaching 99.7 percent, can effectively decompose various toxic and harmful gases, and remove off flavor.

A disinfectant and its disinfecting wet tissue for cleaning and disinfecting human face, hand, skin and mucose are disclosed. Said disinfectant contains antibacterial peptide GK, detergent, and skin-moistening agent. Its advantages are high bactericiding effect (over 99%) to colibacillus, staphylococcus aureus and Pseudomonas aeruginosa, and no irritation.

The invention discloses compound fresh-keeping liquid for chilled duck meat as well as a preparation and the application of the compound fresh-keeping liquid. Per 100 mL of compound fresh-keeping liquid for chilled duck meat comprises nisin accounting for 0.05 to 0.1g, chitosan mother liquor accounting for 5 to 10g, sodium lactate accounting for 4 to 4.8g, clove leaching solution accounting for 4.2 to 6g, cassia bark leaching solution accounting for 3.6 to 6g, pH conditioning agents accounting for proper quantities, and the rest is water. Secondary fresh-keeping treatment comprising soaking and spraying is adopted, the compound fresh-keeping liquid is applied to antisepsis and fresh preservation of chilled duck meat, and the synergistic action among different antiseptic bacteriostatic agents can be exerted; meanwhile, the double fresh-keeping treatment can inhibit the growth of special putrefying bacteria in chilled duck meat more effectively, such as pseudomonas, lactic acid bacteria, aeromonas veronii, brochothrix thermosphacta, enterobacterium and staphylococcus; and in addition, original quality and color of duck meat can be kept, and the conventional shelf life for chilled duck meat can be prolonged to reach about 10 days.

The invention discloses a Pseudomonas aeruginosa and propolis inactivated vaccine for minks, which is prepared from two Pseudomonas aeruginosa strains. The Pseudomonas aeruginosa and propolis divalent inactivated vaccine for the minks is prepared by the following steps of: performing aerobic amplification culturing on a Pseudomonas aeruginosa strain inoculation culture medium; inactivating harvested cultures; then mixing the inactivated cultures in the proportion of 1:1; and adding propolis. The Pseudomonas aeruginosa and propolis inactivated vaccine disclosed by the invention is good in immune effect of preventing hemorrhagic pneumonia of the minks; the protection period of at least five months can be obtained; and the protection rate is at least 80 percent. The Pseudomonas aeruginosa and propolis inactivated vaccine for the minks is good in application prospect.

The invention discloses a method and a kit for detecting aeromonas veronii and relates to a detection technology of aeromonas veronii. The kit for detecting fish pathogenic bacteria, disclosed by the invention, comprises primer pairs of which the sequence is shown as SEQ ID NO: 1-2, and genes from aeromonas veronii are amplified. The detection kit and detection method provided by the invention can accurately and effectively detect the aeromonas veronii, are high in sensitivity, high in specificity, low in time consumption and fast in detection and can be used for rapidly detecting and predicting fish diseases, preventing fish diseases and improving economic benefits.

Gene engineering strain of expression recombinant prawn peptide Pen24 and its use are disclosed. It has excellent antibacterial activity for G+ bacterium, negative bacterium, colicine of antiampicillin and strong pathogenic bacterium, excellent antiviral activity. It can be used to prevent and treat animal and plant virus and as antiseptic agent in use of cosmetics, food and animal forage.

The invention belongs to the technical field of microorganisms and discloses a preparation method and application of a compound microbial substrate modifier with an aquatic pathogen antagonistic characteristic. Two strains namely bacillus subtilis AB-90008-15 and lactobacillus gasseri 88 are provided, and the compound microbial substrate modifier is obtained by strain activation, fermentation culture, centrifugal strain collection and vacuum drying and grinding. The compound microbial substrate modifier has an antagonistic action on aquatic pathogens such as aeromonas hydrophila, aeromonas sobria, aeromonas veronii, aeromonas caviae, Edwardsiella tarda, listonella anguillarum, vibrio parahaemolyticus and vibrio alginolyticus and is capable of degrading carbonaceous organics and nitrogenousorganics in pond sediment, promoting sediment mineralization, reducing contents of total nitrogen, total phosphorus, nitrite, ammonia nitrogen and COD of aquaculture water, promoting virtuous cycle of waternutrients, preventing accumulation of toxic and harmful substances and improving an aquaculture substrate ecological environment.

The invention relates to defensin cathelicidin-PP of polypedates puerensis as well as a gene and application thereof, and belongs to the field of biomedicine. The defensin cathelicidin-PP is a cyclic polypeptide encoded by a gene of defensin of Chinese amphibian polypedates puerensis, and has a molecular weight of 3,366.08 daltons and an isoelectric point of 10.05; the amino acid sequence of the defensin cathelicidin-PP is as shown by SEQ ID NO: 1. A gene GenBank Accession KY610282 for encoding a precursor of the defensin cathelicidin-PP of the polypedates puerensis consists of 615 nucleotide sequences; the nucleotide sequence of the gene GenBank Accession KY610282 is as shown by SEQ ID NO: 2, wherein nucleotides at No. 346 to No. 441 sites are encoding genes of defensin cathelicidin-PP of mature polypedates puerensis. The invention relates to the application of the defensin cathelicidin-PP of the polypedates puerensis to the preparation of a therapeutic medicine for infectious diseases caused by escherichia coli, salmonella paratyphi A, pseudomonas aeruginosa and candida glabrata. The defensin cathelicidin-PP has the obvious effects of inhibiting the growth of a bacterium and a fungus for the escherichia coli, the salmonella paratyphi A, the pseudomonas aeruginosa and the candida glabrata.

The invention specifically discloses a microbial inoculant, in particular to a microbial inoculant prepared from swine borne bacillus licheniformis YHSH-02 and the application thereof in livestock breeding. The culture is preserved in CGMCC, 3#, Yard #1, West Beichen Road, Chaoyang District, Beijing, with the preservation number of CGMCC NO. 15454 and the preservation date of 15th, March, 2018. The bacillus licheniformis YHSH-02 is separated from a swine intestinal tract, can generate rich lipopeptides bacilli, antifungal proteins and the like, and can inhibit escherichia coli, pseudomonas aeruginosa, enterococcus faecalis, enterobacter cloacae, pseudomonas aeruginosa, proteusbacillus vulgaris, staphylococcus aureus, salmonella and clostridium. The bacillus licheniformis YHSH-02 obtained from the swine intestinal tract is a biological control potential culture which is high in preventing and treating efficiency and wide in preventing and treating range and can promote growth obviously,and has a good application and development prospect in non-resistant breeding.

The invention relates to a kit for detecting presence of pathogen pseudomonas aeruginosa in patient samples such as clinical bacterial pneumonia, corneal ulcer, urinary tract infection, septicemia and the like and samples such as cosmetics, environmental monitoring objects and the like, in particular to a kit for early and quickly diagnosing pseudomonas aeruginosa infection by a real-time fluorescence quantitative polymerase chain reaction technique.

The invention discloses an aeromonas veronii rapid detection primer, a kit and application. The aeromonas veronii rapid detection primer is characterized by comprising an outer primer and an inner primer; the outer primer is composed of an outer primer upstream primer shown in SEQ ID NO.1 and an outer primer downstream primer shown in SEQ ID NO.2; the inner primer is composed of an inner primer upstream primer shown in SEQ ID NO.3 and an inner primer downstream primer shown in SEQ ID NO.4. According to the method, the period for detecting aeromonas veronii is short, the detection cost is low, sequencing and standardization of the detection process are achieved, operation is standard, and errors are not prone to being caused. The specificity is good. The dye built-in method is adopted, a reaction tube does not need to be opened after a reaction is finished, results can be observed directly with the naked eyes after the reaction tube is taken out, a follow-up to-be-detected sample is prevented from being polluted by amplified products, bacterial culture does not need to be carried out, and detection can be carried out only through fewer samples such as fish blood.

The invention provides a multiplex PCR (Polymerase Chain Reaction) primer group for avian pathogenic escherichia coli, pasteurella multocida, proteus mirabilis, pseudomonas aeruginosa, salmonella andstaphylococcus aureus, a multiplex PCR detection kit comprising the primer group, and a multiplex PCR detection method using the primer group. The primer group comprises primer pairs PhoA-F and PhoA-Rfor specifically amplifying a gene phoA of the avian escherichia coli, primer pairs KMT-F and KMT-R for specifically amplifying a gene KMT1 of the pasteurella multocida, primer pairs AtpD-F and AtpD-R for specifically amplifying a gene AtpD of the proteus mirabilis, primer pairs PETA-F and PETA-R for specifically amplifying a gene PETA of the pseudomonas aeruginosa, primer pairs InvA-F and InvA-Rfor specifically amplifying a gene InvA of the salmonella, and prier pairs NuC-F and NuC-R for specifically amplifying a gene nuc of the staphylococcus aureus.

The invention provides an aeromonas hydrophila and aeromonas veronii duplex oral sustained-release microsphere vaccine and a preparation method thereof, and belongs to the field of biological pharmacy. With sodium alga acid as a wall material, and inactivated whole bacteria of aeromonas hydrophila and aeromonas veronii as a core material, the aeromonas hydrophila and aeromonas veronii duplex oral sustained-release microsphere vaccine is prepared by an emulsification method. Prussian carp is immunized by the aeromonas hydrophila and aeromonas veronii-calcium alginate microcapsule oral vaccine, so that the serum enzyme activity and the leukocyte phagocytic activity can be significantly improved; the relative protection rate for the prussian carp is 46.7%; the microcapsule is stable in a simulated gastrointestinal condition; relatively good sudden release and slow release are displayed; the bacteria antigenicity in the microcapsule is kept well; and the vaccine safety and the storage stability are relatively good.

The invention provides a pichia pastoris-optimized oncorhynchus mykiss hepcidin gene sequence. Four recombinant plasmids pGAPZAlpha A / pPICZAlphaA-hep and pGAPZAlphaA / pPICZAlphaA-mhep are constructed by utilizing hepcidin genes or segments thereof, and are respectively electrotransformed into pichia pastoris GS115 and KM71H host cells to obtain a recombinant pichia pastoris strain, the recombinant pichia pastoris strain is subjected to culture expression to obtain expression polypeptide with the multi-bacteria resistance activity. The expression polypeptide has stronger bacteriostatic activity on escherichia coli, staphylococcus aureus and a part of fish pathogenic bacteria (vibrio parahaemolyticus 1.2164, aeromonas veronii X-1-06909, aeromonas hydrophila HA-336, and aeromonas veronii CL0901).

The invention provides enterococcus faecium R8 that is screened and separated from a crucian intestinal tract and has safe and prebiotic characteristics. The enterococcus faecium is collected in ChinaGeneral Microbiological Culture Collection Center (CGMCC) of China Committee for Culture Collection of Microorganisms on January 17, 2018 and has a collection number of CGMCC NO. 15229. The enterococcus faecium is high in heat resistance, acid-base resistance and stress resistance and more significant in probiotic performance, has a relatively obvious inhibiting effect on common aquatic pathogenic bacteria such as aeromonas veronii, staphylococcus haemolyticus, vibrio parahaemolyticus and vibrio vulnificus and can be widely applied to aquaculture as a feed additive.

The invention discloses a preparation method of drug-resistant pseudomonas aeruginosa broad-host range phage, which relates to a preparation method of phage and solves the problem of poor separation and purification effect in the traditional separation method of the pseudomonas aeruginosa phage. The method comprises the following steps: 1, selecting a sewage sample; 2, preparing a lysis solution; 3, carrying out mixed cultivation on the lysis solution and a pseudomonas aeruginosa host cell suspension to obtain a mixed solution; 4, preparing a single phage plaque; and 5, inoculating the phage into a pseudomonas aeruginosa-containing culture medium, culturing until the pseudomonas aeruginosa is completely dissolved, and finally filtering. The preparation method of pseudomonas aeruginosa phage has the advantages of simple operation, good separation and purification effect and no phage breakage. The prepared drug-resistant pseudomonas aeruginosa broad-host range phage can be used for manufacturing autoimmune (vaccines) biological agents for treating diseases caused by the infection of drug-resistant pseudomonas aeruginosa, can be especially used as a topical spray for treating epidermal infectious diseases, and can avoid the drug-resistant pseudomonas aeruginosa produced by the use of antibiotics.

The invention provides application of a medicine containing catechin shown in formula (I) or catechin polymorphs to preparation of antibacterial medicines. Through experiment research, the catechin is wide in antibacterial spectrum and has exact inhibiting effect on various pathogenic microorganisms such as staphylococcus aureus, escherichia coli, candida albicans, pseudomonas aeruginosa and hemolytic streptococcus; meanwhile, the catechin has good selectivity and has no irritation to normal flora and vagina mucosa, thereby being promising in application prospect on preparation of the antibacterial medicines. Formula (I).

Disclosed is an externally-used Mongolian medicament for treating skin diseases, which is prepared from equal weight amount of cape jasmine, toosendan fruit and chebula fruit through alcohol extraction and water extraction. Test results show that, the medicament can be used for eradicating fungus and bacteria that cause the infectious skin diseases, thus can be used for treating tinea corporis.

The invention discloses a marine fungi penicillium thomii, an extract and application thereof. A marine fungi penicillium thomii (penicillium thomii) FS77 was preserved at China Center for Type Culture Collection (CCTCC) at Wuhan University of Wuhan City of China on November 24th, 2011 and the preserving number is CCTCC M201414. The fermentation extract has restraining effects on staphyloccocus aureus, bacillus subtilis and pseu domonas aeruginosa. Simultaneously, the fermentation extract has resisting effects on plant pathogen fungi alternaria alternate, colletotrichum gloeosporioides and cylindrocladium scoparium. Accordingly, the marine fungi penicillium thomii provides selectable chemicals for studying and developing new antibacterial chemicals and biopesticides, and provides scientific evidences for developing natural active materials of a marine fungi source.

Antimicrobial peptide LF-TH and its Escherichia coli recombinant preparation method. It solves the problem of low antibacterial activity of the existing antibacterial peptides. The amino acid sequence of the antimicrobial peptide LF-TH includes 33 amino acids, its amino acid sequence is shown in SEQ ID No.1, and its coding gene sequence is shown in SEQ ID No.2. Methods: 1. Design the antimicrobial peptide LF-TH and the coding gene of the antimicrobial peptide LF-TH; 2. Link the coding gene of the antimicrobial peptide LF-TH to the plasmid and construct a recombinant Escherichia coli expression vector, transform E.coliBL21(DE3), and The fusion protein is obtained after induced expression and purification, the fusion protein is cleaved by enterokinase to release the antimicrobial peptide LF-TH, and the antimicrobial peptide LF-TH is obtained after purification. The antibacterial peptide LF-TH of the present invention has high antibacterial activity, and has obvious inhibitory effect on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Salmonella typhimurium.

An antiepidemic bactericide in the form of liquid, solid, or combustible fragrant refreshing agent for killing the pathogenic bacteria including dysentery bacillus, Staphylococcus aures, anthrax bacillus, etc and refreshing indoor air is prepared from dahurian angelica root, etc.

Streptomyces amritsarensis N1-32, a micro-ecological preparation thereof and a preparing method thereof are provided. The Streptomyces amritsarensis N1-32 is deposited in the China Center for Type Culture Collection with an accession number of CCTCCNO:M 2019381. The Streptomyces amritsarensis N1-32 can effectively suppress growth of common fish pathogenic bacteria, especially Aeromonas veronii. Application of the micro-ecological preparation for grass carp liver cells proves that the Streptomyces amritsarensis N1-32 is harmless and nontoxic to fish. The micro-ecological preparation is utilizedas feed to feed grass carp, crucian, and other types of freshwater fish, and a result shows that the growth rate and the feed efficiency of the grass carp are increased, and immunity of freshwater fish is boosted, and therefore, resistance of freshwater fish, such as grass carp, to fish pathogenic bacteria can be significantly improved.