1819 results about "Pseudomonas aeruginosa" patented technology

The present invention relates to nanosilver-containing antibacterial and antifungal granules ("NAGs"). The NAGs have longlasting inhibitory effect on a broad-spectrum of bacteria and fungi, which include, but are not limited to, Escherichia coli, Methicillin resistant Staphylococcus aureus, Chlamydia trachomatis, Providencia stuartii, Vibrio vulnificus, Pneumobacillus, Nitrate-negative bacillus, Staphylococcus aureus, Candida albicans, Bacillus cloacae, Bacillus allantoides, Morgan's bacillus (Salmonella morgani), Pseudomonas maltophila, Pseudomonas aeruginosa, Neisseria gonorrhoeae, Bacillus subtilis, Bacillus foecalis alkaligenes, Streptococcus hemolyticus B, Citrobacter, and Salmonella paratyphi C. The NAGs contain ground stalk marrow of the plant Juncus effusus L. which has been dispersed with nanosilver particles. The nanosilver particles are about 1-100 nm in diameter. Each of the nanosilver particles contain a metallic silver core which is surrounded by silver oxide. The present invention also provides a process for making the NAGs. The NAGs can be used in a variety of healthcare and industrial products. Examples of the healthcare products include, but are not limited to, ointments or lotions to treat skin trauma, soaking solutions or cleansing solutions for dental or women hygiene, medications for treating gastrointestinal bacteria infections, sexual related diseases, and eye diseases. Examples of industrial products include, but are not limited to, food preservatives, water disinfectants, paper disinfectants, construction filling materials (to prevent mold formation).

It has been discovered that fluids viscosified with viscoelastic surfactants (VESs) may have their viscosities reduced (gels broken) by the direct or indirect action of a biochemical agent, such as bacteria, fungi, and/or enzymes. The biochemical agent may directly attack the VES itself, or some other component in the fluid that produces a by-product that then causes viscosity reduction. The biochemical agent may disaggregate or otherwise attack the micellar structure of the VES-gelled fluid. The biochemical agent may produce an enzyme that reduces viscosity by one of these mechanisms. A single biochemical agent may operate simultaneously by two different mechanisms, such as by degrading the VES directly, as well as another component, such as a glycol, the latter mechanism in turn producing a by-product (e.g. an alcohol) that causes viscosity reduction. Alternatively, two or more different biochemical agents may be used simultaneously. In a specific, non-limiting instance, a brine fluid gelled with an amine oxide surfactant can have its viscosity broken with bacteria such as Enterobacter cloacae, Pseudomonas fluorescens, Pseudomonas aeruginosa, and the like.

The invention relates to a compound microbial agent which comprises compound bacteria for decomposing kitchen wastes and auxiliary materials for solid fermentation, wherein the compound bacteria comprise 10-15 parts of cellulose degradation bacteria, 15-30 parts of starch degradation bacteria, 15-20 parts of protein degradation bacteria, 15-20 parts of grease degradation bacteria, 10-15 parts of bacillus subtilis and 5-15 parts of pseudomonas aeruginosa; the auxiliary materials comprise 50-60 kg of wheat bran, 15-20 kg of bean pulp, 0.4-0.6 kg of magnesium sulfate, 0.4-0.6 kg of potassium dihydrogen phosphate, 0.1-0.2 kg of disodium hydrogen phosphate and 8-10 kg of glucose. The inoculation amount of the compound bacteria in the auxiliary materials is 0.8%-1%, and the compound bacteria is subjected to solid fermentation to obtain the compound microbial agent. The compound microbial agent has a good decomposing effect on greases, proteins and fiber substances and can be tolerant to salt with a certain concentration; optimally, the degradation rate can reach more than 90%.

Described are synergistic antimicrobial-fragrance compositions including broad spectrum antimicrobial compositions containing salicylaldehyde and at least one organoleptically-compatible antimicrobial synergism cofactor substance. The weight ratio range of salicylaldehyde:synergism cofactors substance is from 1:10 up to 10:1. The cofactor substance is such that the degree of synergism of the resultant mixture is defined according to the IFF Antimicrobial Synergism Test wherein the difference between the actual and expected antimicrobial values of the mixture is greater than or equal to a multiple of (i) 0.05 and (ii) the expected antimicrobial value of the mixture. Cofactor substances include phenolics such as cresol, caravacrol and thymol; ethyl vanillin; benzyl alcohol; indol; beta-orcinol; and terpinenol-4. Microorganisms against which the synergistic compositions are effective include:Escherichia coli;Enterococcus hirae;Pseudomonas aeruginosa;Staphylococcus aureus; andSaccharomyces cerevisae.The compositions have application in all-purpose cleaning compositions, gel-type toilet rim articles, liquid-type toilet rim articles, personal shower cleaning compositions, and body and hair care products including shower gel compositions, shampoo compositions and foam bath compositions.

The invention relates to an andrographolide derivative, which has the structure as shown in a general formula I: wherein, R1, R2 and R3 are the same or different hydrogen, substituted or non-substituted organic acid radical, inorganic acid radical, alkyl, aryl or heteroaryl, while at least one of R1, R2 and R3 is R-lipoic acid or S-lipoic acid or the mixture of R-lipoic acid and S-lipoic acid or the corresponding dihydrolipoic acid or acetylcysteine radical of R-lipoic acid or S-lipoic acid; the derivative has good anti-tumor effect, and the derivative can cause apoptosis of tumor cells, directly eliminate gram positive bacteria, staphylococcus aureus and sensitivities MRSA5676 and MRSA5677, inhibit the QS system of gram negative bacteria and pseudomonas aeruginosa and inhibit and damage the formation of the bio-film of pseudomonas aeruginosa; the product has prominent hypoplycemic effect and is suitable for the preparation of the medicines that can cure cancers, inflammations, diabetes mellitus and bacterial and viral infection.

The invention relates to nano-silver solution with the nano-silver concentration between 250 and 3,200ppm and the average sliver grain diameter between 2 and 12nm. The nano-silver solution also comprises medicine accessory level dispersing agents and medicine level reducing agents. The preparation method mainly comprises the following steps of: dissolving the medicine accessory level dispersing agents into deionized water to prepare dispersing agent solution; adding and dissolving silver nitrate to obtain solution I; taking and adding the medicine accessory level reducing agent solution into the solution I; mixing the medicine accessory level reducing agent solution with the solution I to obtain solution II; placing the solution II under an ultraviolet lamp to be irradiated; and stirring the solution II for reaction to obtain the nano-silver solution. The nano-silver solution has stronger antibiosis performance. When the nano-silver solution is diluted to 10ppm to detect and evaluate the antibiosis effect on staphylococcus aureus, colibacillus and pseudomonas aeruginosa, the antibiosis efficiency reaches higher than 99.9 percent in two minutes. The preparation method has simple process, can avoid the use of various strong acid, strong base and strong reducing agents and has good medical safety.

The invention relates to a method for degrading the oily sludge of a well site by using microorganisms. The method comprises the following steps of: obtaining four degrading bacteria namely pseudomonas aeruginosa, micrococcus luteus, bacillus subtilis and acinetobacter johnsonii from the oily sludge of the well site in the loess tableland region through culturing, separation, screening, domestication and mutation, and mixing the four degrading bacteria according to the weight ratio of 1:1:1:1 to obtain a microorganism flora for degrading the oily sludge, wherein the initial pH value equals to6, the weight ratio of carbon to nitrogen equals to 100:3, and the weight ratio of carbon to phosphorus equals to 100:0.6, the mass concentration of oils in a treatment system added with mixed bacteria is reduced from 17214 mg/kg to 1257 mg/kg within 72 h in a laboratory, and the degradation rate is 92.7%. After the oily sludge with 10.55% initial oil content is experimentally treated for 56 daysin the well site, the removal rate of oil matters can reach 89.1%, and the treatment effect is obvious.

A formulation comprising molecular arrangements capable of penetrating pores in a barrier, owing to penetrant adaptability, despite the fact that the average diameter of said pores is smaller than the average penetrant diameter, provided that the prenetrants can transport agents or else enable agent permeation through the pores after penetrants have entered pores, characterized in that the formulation comprises at least one consistency builder in an amount that increases the formulation to maximally 5 Nm/s so that spreading over, and retention at, the application area is enabled and/or at least one antioxidant in an amount that reduces the increase of oxidation index to less than 100% per 6 months and/or at least one microbiocide in an amount that reduces the bacterial count of 1 million germs added per g of total mass of the formulation to less than 100 in the case of aerobic bacteria, to less than 10 in the case of entero-bacteria, and to less than 1 in the case of Pseudomonas aeruginosa or Staphilococcus aureus, after a period of 4 days.

The invention comprises a substrate for detection of micro-organisms, wherein said substrate comprises a set of molecular markers linked, optionally with linker molecules or moeieties, to a di-, or tripeptide consisting of amino acids X1 and X2, or X1, X2 and X3, in which one of them, for example X1, is a D-amino acid and the others, for example X2 and X3, may be any D- or L-amino acid. Said substrate preferably is used for the detection of Bacillus anthracis. Alternatively, the invention is directed to a substrate for detection of micro-organisms, more specifically P. aeruginosa, wherein said substrate comprises a set of molecular markers linked, optionally with linker molecules or moeities to a tri- tetra or pentapeptide consisting of glycine amino acids. The invention further comprises methods for detection of micro-organisms, specifically Bacillus anthracis and Pseudomonas aeruginosa, with the substrates of the invention and use of the substrate(s) in such a method.

The invention provides a Bacillus coagulans HEW-B379 with a probiotic effect. The above strain is named as HEW-B379, and the preservation number of the strain is CGMCC No.12553. The Bacillus coagulans HEW-B379 has a substantial probiotic property, and can effectively inhibit growth breeding of enteropathogenic Escherichia coli, Staphylococcus aureus, Salmonella typhi, salmonella, Shigella, Proteus species, Shewanella putrefaciens and Pseudomonas aeruginosa. The Bacillus coagulans HEW-B379 has strong stress resistance, can resist high temperature and simulated gastric juice and simulate bile salt environment, can keep the survival rate of 99-100%, and can effectively adjust microbial balance of animal intestinal tracts, inhibit growth of harmful microbes, promote nutrition absorption of animals, improve the conversion rate of a feed and improve the productivity of the animals.

The invention relates to a preparation method of rhamnolipid biological fermentation liquid. The method comprises the following steps: extracting and purifying Pseudomonas aeruginosa obtained from petroleum-contaminated soil, and obtaining Pseudomonas aeruginosa VTS-1 (Pseudomonas sp. -1) The preservation number is: CGMCC2200, and the specific fermentation and cultivation steps are as follows: A. Strain VTS-1 is cultured in shake flasks; B. Primary fermentation cultivation; C. Secondary fermentation cultivation; D. Fermentation cultivation. The process of the method has the advantages of high yield, high efficiency, high loading coefficient, short fermentation cycle, simple process, reduced overall product cost, and is completely suitable for industrial production, and has played a role in the promotion and large-scale application of rhamnolipid biosurfactants. important role.

The invention relates to a biological agent for processing industrial sewage, a preparation method and application. The biological agent is prepared by the following raw materials in percentage by weight; 25% of rhodococcus, 20% of bacillus subtilis, 15% of pseudomonas aeruginosa, 10% of saccharomyces cerevisiae, 10% of nitrosobacteria, 10% of bacillus megatherium, 5% of aspergillus niger and 5% of paracoccus denitrificans. According to the biological agent provided by the invention, the strains are reasonably matched, symbiotic and coordinative and out of antagonism; after being added to a sewage treatment system, the trains are good in effect of degrading macromolecular toxic and harmful substances which are hardly degraded, such as phenols, aromatichydrocarbons, ammonia nitrogen and phosphorus; and the biological agent has unique effect of processing high-concentration organic sewage and high-ammonia nitrogen sewage which are hardly processed by conventional activated sludge process.

The present invention provides pharmaceutical compositions in the form of relatively high molecular weight biocompatible polymers such as polyethylene glycol, optionally supplemented with a protective polymer such ad dextran and/or essential pathogen nutrients such as L-glutantine. Also provided are methods for preventing or treating gut-derived sepsis attributable to intestinal pathogens such as Pseudomonas aeruginosa by administering high molecular weight polyethylene glycol as well as methods for monitoring the administration of high molecular weight polyethylene glycol, such as in methods of preventing, ameliorating or treating microbe-induced epithelial disorders, as exemplified by gut-derived sepsis. Frequently, gut-derived sepsis arises as a complication in mammals recovering from surgical intervention or suffering from a disease or disorder, providing indications of suitable animals to receive preventative treatment. Finally, the invention provides a composition comprising infant formula and polyethylene glycol and methods for using that composition.

The invention belongs to the treatment field of heavy metal polluted wastewater and particularly discloses immobilization pseudomonas aeruginosa. The immobilization pseudomonas aeruginosa comprises pseudomonas aeruginosa live bacteria and a mixing carrier used for fixing the bacteria, wherein the mixing carrier is a spherical object embedded with the live bacteria and mainly comprises the following compositions of polyvinyl alcohol, calcium alginate and multi-wall carbon nano-tube in the mass ratio of 50-60:30-40:1.6-6. The preparation method of the immobilization pseudomonas aeruginosa comprises the following steps of: firstly, preparing a mixed solution by mixing the polyvinyl alcohol, the calcium alginate, the multi-wall carbon nano-tube and water, uniformly heating and stirring, and obtaining an immobilization reagent by standing; secondly, adding a pseudomonas aeruginosa into an immobilization reagent to obtain a bacteria solution, and dripping the bacteria solution into a calcium nitration solution to obtain an immobilization pseudomonas aeruginosa microsphere; and finally, obtaining the immobilization pseudomonas aeruginosa through frozen-thawed treatment. The immobilization pseudomonas aeruginosa can reduce hexavalent chromium to trivalent chromium, has the advantages of high efficiency, favorable effect, also simple operation and no secondary pollution.

The invention provides a method for synergistically degrading oil producing wastewater by using petroleum degrading bacteria. The method comprises the following steps: (1) inoculating purified single pseudomonas aeruginosa, bacillus subtilis, pseudomonas mendocina and acinetobacter baumannii strains into a beef extract-peptone medium, and culturing for 18 hours so as to obtain seed bacterium liquid; (2) preparing an acclimation culture solution of the seed bacterium liquid; (3) adding the prepared acclimation culture solution to a fermentation medium according to a single strain bacterium liquid isopyknic ratio, and culturing; (4) putting fermented bacterium liquid in an aeration tank so as to treat the oil producing wastewater. The composite high-efficiency oil removal bacterial strain can be used for degrading more than 94% of hydrocarbon compounds in the oil producing wastewater; the selected strains are respectively high in crude oil degradation rate and have synergistic effect; the combined degradation efficiency of several bacteria for crude oil is considerably higher than the simple superimposed effect of the single effect of the bacteria.