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Compound microbial agent as well as processing method and application thereof

A composite bacterial agent and treatment method technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of reducing the decomposition effect and achieve a good decomposition effect

Active Publication Date: 2014-04-30
BEIJING GREEN ENERGY ENVIRONMENTAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

Due to the different characteristics of food waste in my country and foreign countries, the mature microbial strains used in the treatment of food waste in foreign countries cannot give full play to their original biological characteristics during the treatment of food waste, which greatly reduces the decomposition effect. Therefore, microbial strains imported from abroad cannot be directly applied, and independent innovation of microbial compound bacterial agents is required to achieve better treatment effects and resource utilization effects

Method used

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  • Compound microbial agent as well as processing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1 The preparation embodiment of microbial composite bacterial agent of the present invention

[0017] Culture of various strains

[0018] (1) Cellulolytic flavinogenic Cellulomonas with the preservation number CICC23686, the amylase-producing Bacillus subtilis with the preservation number CICC10066, and the protease-producing Bacillus subtilis with the preservation number CICC10071 were deposited in the China Industrial Microorganism Culture Collection Center. The lipase-producing Bacillus licheniformis with the number CICC21085, the Bacillus subtilis with the preservation number CICC10210, and the Pseudomonas aeruginosa with the preservation number CICC21100 were inoculated in LB medium, shaken at 37°C for 20 to 24 hours, and then the proportion was 15:15. :20:20:11:9 mixed.

[0019] (2) Add 800mL of the above-mentioned mixed bacteria liquid to the solid fermentation auxiliary material, the inoculum volume to weight ratio is 0.8%-1%, and the humidity is adj...

Embodiment 2

[0021] Embodiment 2 The waste degradation rate test of compound microbial strain of the present invention

[0022] This example is the degradation test of the composite microbial bacterial agent prepared in Example 1 to kitchen waste, and the degradation treatment effect of the bacterial agent is reflected by weight loss efficiency:

[0023]

[0024] in,

[0025] A: The total weight of garbage, auxiliary materials and bacterial agents put into the biochemical processor before treatment, kg;

[0026] B: The total weight of the remaining materials in the biochemical processor after treatment, kg;

[0027] C: The total weight of garbage input into the biochemical processor, kg.

[0028] In this example, a biochemical treatment machine (LSNL-FW-500A) with a daily waste treatment capacity of 500kg manufactured by green energy is used. Add 25kg of the composite microbial agent prepared in Example 1 and 2.5kg of glucose into a kitchen waste disposer pre-added with 350kg of auxi...

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Abstract

The invention relates to a compound microbial agent which comprises compound bacteria for decomposing kitchen wastes and auxiliary materials for solid fermentation, wherein the compound bacteria comprise 10-15 parts of cellulose degradation bacteria, 15-30 parts of starch degradation bacteria, 15-20 parts of protein degradation bacteria, 15-20 parts of grease degradation bacteria, 10-15 parts of bacillus subtilis and 5-15 parts of pseudomonas aeruginosa; the auxiliary materials comprise 50-60 kg of wheat bran, 15-20 kg of bean pulp, 0.4-0.6 kg of magnesium sulfate, 0.4-0.6 kg of potassium dihydrogen phosphate, 0.1-0.2 kg of disodium hydrogen phosphate and 8-10 kg of glucose. The inoculation amount of the compound bacteria in the auxiliary materials is 0.8%-1%, and the compound bacteria is subjected to solid fermentation to obtain the compound microbial agent. The compound microbial agent has a good decomposing effect on greases, proteins and fiber substances and can be tolerant to salt with a certain concentration; optimally, the degradation rate can reach more than 90%.

Description

technical field [0001] The invention relates to a microbial composite bacterial agent, in particular to a microbial composite bacterial agent for decomposing kitchen waste, as well as its treatment method and application. Background technique [0002] At present, a considerable proportion of food waste is illegally collected and transported to feed pigs or enter underground workshops to extract "gutter oil". After being mixed with other domestic waste, the "mixed waste" produced hinders the effective recycling and recycling of domestic waste. use. my country's food waste has the characteristics of "four excesses and one miscellaneous", that is, large amount, high water, high oil, high salt and complex components. It is one of the most difficult food waste to deal with. Due to the different characteristics of food waste in my country and foreign countries, the mature microbial strains used in the treatment of food waste in foreign countries cannot give full play to their ori...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09B3/00C05F11/08C12R1/01C12R1/125C12R1/10C12R1/385
Inventor 宋建国
Owner BEIJING GREEN ENERGY ENVIRONMENTAL ENG
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