448 results about "Lipase" patented technology

The invention relates to the technical field of functional lipid preparation, in particular to high-purity nannochloropsis oculata polar lipid enriched by an enzymic method and a preparation method of the high-purity nannochloropsis oculata polar lipid. The method comprises the following steps: specifically and selectively hydrolyzing triglyceride by using immobilized Lip2 lipase to generate glycerol and free fatty acid, retaining and enriching polar lipid components, and carrying out centrifugal filtration and molecular distillation to obtain the high-purity nannochloropsis oculata polar lipid. The invention provides a method for preparing high-purity polar lipid by performing enzymolysis on nannochloropsis oculata oil by using lipase Lip2 produced by yarrowia lipolytica, which maximally retains the polar lipid and obtains a high-purity polar lipid product through the specific catalytic action of Lip2 lipase. The method which is mild in reaction condition, environment-friendly, simple in step and high in purity is provided for efficient enrichment of the nannochloropsis oculata polar lipid, the prepared polar lipid product is extremely high in purity, and the blank of efficient preparation of the nannochloropsis oculata polar lipid in the prior art is filled.

The invention relates to the technical field of active peptides, in particular to a myocardial active peptide capable of relieving heart failure and protecting heart and a preparation method thereof. The method takes pig hearts as raw materials, and sequentially comprises the following steps: pretreating the pig hearts, adding water, and homogenizing; adding alkaline protease to carry out first constant-temperature enzymolysis; adding neutral protease and flavourzyme to carry out second constant-temperature enzymolysis; then lipase is added for third-time constant-temperature enzymolysis, and enzyme deactivation and cooling are carried out; and finally, adding activated carbon into the enzymatic hydrolysate, filtering with diatomite, and spray-drying to obtain the myocardial active peptide. According to the method, the yield and the purity of the active peptide are remarkably improved through a synergistic process of three-step enzymolysis and activated carbon purification, the obtained product is rich in small molecule peptide with a definite heart protection function, and a new way is provided for high-value utilization of pig heart resources and development of functional products for preventing and treating heart failure.

The invention relates to the technical field of genetic engineering, in particular to a recombinant vector, an expression plasmid, a recombinant schizochytrium limacinum mutant and a method for increasing the DHA content of microorganisms. The LIP1 gene for coding lipase in the microbial lipid droplet protein is knocked out through a homologous recombination technology, and the production of lipase in the microbial lipid droplet can be effectively inhibited, so that the hydrolysis process of triglyceride of polyunsaturated fatty acid stored in the lipid droplet to glycerol and free fatty acid in the later stage of microbial culture is blocked, and DHA is prevented from being lost from a TAG repository; experimental verification shows that in microorganisms represented by schizochytrium limacinum, the DHA content can be increased by 21.8% compared with that of a wild type, the yield and purity of DHA are increased, the stability of the microbial fermentation process is guaranteed, and application and popularization are facilitated.

The invention relates to the technical field of food, in particular to geobacillus stearothermophilus cKM-Geo, application of the geobacillus stearothermophilus cKM-Geo, a shrimp shell oligosaccharide peptide compound and a preparation method of the shrimp shell oligosaccharide peptide compound. The invention provides geobacillus stearothermophilus cKM-Geo, the strain can grow in an oxygen-free environment, ventilation is not needed in the process, and proteolytic enzyme, cellulase, lipase and chitin deacetylase can be highly secreted and expressed; the invention further provides a preparation method for preparing the shrimp shell oligosaccharide peptide by utilizing geobacillus stearothermophilus cKM-Geo fermentation coupling steam explosion treatment, chitin and protein in the shrimp shell are converted into water-soluble oligopeptide and oligosaccharides, the water-soluble oligopeptide and oligosaccharides are separated from calcium carbonate in the shrimp shell, acid and alkali do not need to be used, in the process, waste water is little, pollution is little, and the method is suitable for industrial production. The resource utilization rate is high.

The invention discloses a degradable packaging material capable of controlling the release rate of fertilizer nutrients and a preparation method of the degradable packaging material, and relates to the technical field of agricultural packaging. The material is composed of a base material layer, a slow-release regulation and control layer and a degradation promotion layer, the base material layer provides mechanical support and barrier property, the slow-release regulation and control layer realizes accurate release of nutrients, and the inner surface of the degradation promotion layer is loaded with lipase and carboxymethyl cellulose sodium or a polyvinyl alcohol binder to accelerate degradation. The preparation adopts a multi-layer co-extrusion blow molding blank making and enzyme solution spraying post-treatment process, and the steps are clear and controllable. The material is adjustable in nutrient release, degradable and residue-free after use, suitable for various crop fertilization scenes, capable of improving the fertilizer utilization rate and reducing environmental pollution, and remarkable in practical value, and the mechanical property meets the packaging and transportation requirements.

The invention relates to the technical field of novel fertilizers, and particularly discloses an organic water-soluble fertilizer as well as a preparation method and application thereof. The organic water-soluble fertilizer provided by the invention is prepared from the following components: kitchen waste organic mother liquor, an anti-corrosion synergist, a stress-resistant synergist, a growth-promoting synergist, a dispersing agent and polyethylene glycol-400, the kitchen waste organic mother liquor is prepared by sequentially carrying out wet and hot hydrolysis, amylase enzymolysis, protease enzymolysis and lipase enzymolysis on a kitchen waste solid phase and a kitchen waste liquid phase; the preservative synergist is prepared by mixing a natamycin solution, tea polyphenol and chitosan oligosaccharide; the stress-resistant synergist is prepared by mixing complex enzyme glycoside, a plantain herb extract and gamma-aminobutyric acid; the growth promoting synergist is formed by mixing mineral source potassium fulvate and gluconic acid. The organic water-soluble fertilizer prepared by the invention has good corrosion resistance and product stability, has a good growth promoting effect on crops, and can reduce the number of diseased plants with successive cropping obstacles and improve the yield of the crops in a soil environment with successive cropping obstacles.

The invention belongs to the technical field of fermentation engineering, and particularly relates to a staphylococcus pseudoxylosus strain and application of the staphylococcus pseudoxylosus strain in enhancing the flavor of fermented food, and the preservation number of the staphylococcus pseudoxylosus strain is GDMCC NO: 66815. The strain can produce lipase and protease, and can significantly enhance the content of flavor substances such as ketones, alcohols, esters, pyrazine furans and the like in fermented food. And inoculating the staphylococcus pseudoxylosus E2 into food to be fermented, and fermenting at 25-40 DEG C for 5-35 days. Compared with natural fermentation, the content of flavor substances in fermented food can be remarkably increased, and the method is suitable for commercialization.

The invention relates to the technical field of separation and purification, in particular to a method for recovering cis-15-tetracosenoic acid from an acer truncatum seed oil byproduct, which comprises the following steps: selecting the acer truncatum seed oil byproduct, crushing, sieving, washing, adding modified attapulgite and beta-cyclodextrin, stirring and adsorbing at constant temperature, and filtering; preparing the modified attapulgite; adding a KOH-EtOH solution containing lipase and polyethylene glycol into a pretreated product for refluxing, and adding a saturated saline solution for refluxing; standing and layering the reaction liquid, and extracting, acidifying, washing, drying and distilling an organic phase to obtain mixed fatty acid; and carrying out composite purification to obtain a pure cis-15-tetracosenoic acid product. According to the method, the purity and the extraction rate of the cis-15-tetracosenoic acid are remarkably improved, the oxidation, heat and storage stability of the product is enhanced, the color is improved, and the comprehensive application performance is better.

The invention relates to the technical field of deep processing of vegetable fat, in particular to a preparation method and application of squalene-containing macadamia nut oil. Through the synergistic effect of directional enzymolysis conversion and low-temperature step-by-step extraction and the combined effect of the three enzymes (cellulase destroys cell walls, lipase releases grease and beta-glucosidase removes inhibitors, and the three enzymes form a wall breaking-oil releasing-synergistic synergistic reaction) by utilizing a compound enzyme which is fixedly matched and proportioned. The compound enzyme can be used for synchronously treating cellulose, lipid and glycoside substances, so that the release efficiency of squalene is improved; and step-by-step extraction is utilized to reduce extraction impurities, so that the content of squalene in the macadamia nut oil can be increased, and the extraction and enrichment of squalene in the macadamia nut meat can be realized.

The invention relates to the technical field of laundry detergents, and particularly discloses a concentrated laundry detergent capable of stably dispersing microcapsule essence and a preparation method of the concentrated laundry detergent. An organic polyurethane compound with a specific carbamate structure (the general formula is RNHCOOR ') is adopted as a suspending agent, the compound constructs a three-dimensional network structure through intermolecular hydrogen-bond interaction, the hydrogen bond donor / acceptor density of a carbamate unit is higher than that of existing suspension systems such as polyamide and hydrogenated castor oil, and the system is endowed with remarkable thixotropic characteristics. Under the action of shearing force, the network is dissociated, and the viscosity is reduced; and after the shearing force is removed, the hydrogen bond is quickly reconstructed, the viscosity rises again, rheological yield stress is generated, and stable anchoring of the high-load suspended matter is achieved. Meanwhile, due to the characteristic of high melting point of the organic polyurethane compound, the high-temperature processing condition can be tolerated, and the phenomenon of hot roughing is avoided. In addition, the hydrolysis resistance of the carbamate bond ensures the long-term stability of the suspension system in an environment with pH of 6-10 and in the presence of lipase.

A hard surface cleaning composition comprising: (a) 0.1 to 60 wt % of a surfactant system; (b) 0.1 to 40 wt % of a solvent, wherein the solvent is selected from the group consisting of a lower alkanol, a benzyl alcohol, a lower alkyl ether, a glycol, an aryl glycol ether, a lower alkyl glycol ether, a glycerol ketal, an ester, a hydrocarbon / ester blend, a dibasic ester, a linear alcohol ethoxylate, a branched alcohol, oxo alcohol ethoxylate, and combinations thereof; and (c) optionally 0.001 to 10 wt % of a lipase.

The invention relates to the technical field of plant genetic engineering, and particularly provides cloning of a GbGELP25D gene of gossypium barbadense and application of the GbGELP25D gene of gossypium barbadense. According to the invention, the GbGELP25D gene with a full length of 1092 bp is cloned from the sea island cotton variety Xinhai No. 7 for the first time, and the gene encodes a secretory lipase protein with a signal peptide and is positioned in an extracellular gap. Through bioinformatics analysis, phylogenetic classification, protein structure modeling and signal peptide function verification, it is clear that the gene belongs to a plant GELP family. Furthermore, a virus-induced gene silencing technology is utilized to prove that the silent GbGELP25D can obviously enhance the resistance of cotton to verticillium wilt, and the mechanism of the silent GbGELP25D is closely related to activation of ethylene synthesis and signal channels and induction of expression of disease-resistant related genes. The resistance gene provided by the invention enriches gene resources of cotton verticillium wilt resistance breeding, and has important theoretical significance and application value.

The invention discloses an esterification reaction lipase screening method based on covalent bond docking, and belongs to the technical field of protein docking simulation. According to the method, parameters of an acylase intermediate are constructed, and then covalent docking and energy screening are performed on the acylase intermediate, so that prediction of lipase reaction activity and substrate selectivity is realized, and the defects that carbonyl carbon cannot be automatically identified, Ser-C covalent bonds cannot be established, double bonds / bond angles cannot be managed, and the reaction efficiency is low in traditional docking are overcome. The problems that in a covalent system, simulation of a nucleophilic attack path lacks a unified distance, angle and dihedral angle constraint system, and automatic and high-throughput screening of multiple lipases and multiple substrates is difficult to achieve are solved.

The application discloses a biological enzyme degreasing treatment method for reducing down feather peculiar smell, which comprises the following steps: raw material down feather pre-finishing, pre-wetting, pulse negative pressure penetration, controlled protease pretreatment, lipase deep degreasing, intermittent low-energy ultrasonic assisted migration, warm water rinsing, cyclodextrin inclusion peculiar smell, weak acid recovery, limited fat supplementing and dehydration low-temperature drying. The application discharges the air in the down feather through pulse negative pressure and promotes the treatment liquid to enter the deep layer of the down feather, improves the contact ability of the lipase and the residual lipid through the protease pretreatment, promotes the oil hydrolysis and migration through the lipase and low-energy ultrasonic, and reduces the residual fat and the aftertaste risk through the cyclodextrin inclusion of volatile peculiar smell substances such as fatty acids, aldehydes and ketones, the weak acid recovery and the fat supplementing treatment, reduces the damage of strong alkali washing to the down feather, maintains the down feather fluffiness, softness and fiber integrity, and is suitable for industrial degreasing and peculiar smell removing treatment of the down feather.

The application discloses a thermophilic bacteria-derived esterase mutant and application thereof. The application provides a protein, wherein the amino acid residue at the 211th position in the amino acid sequence shown in SEQ ID NO: 4 is mutated, and the amino acid residues at other positions are not changed, so that a protein with PBAT hydrolytic enzyme activity is obtained; or the amino acid residues at the 211th and 256th positions in the amino acid sequence shown in SEQ ID NO: 4 are mutated, and the amino acid residues at other positions are not changed, so that a protein with PBAT hydrolytic enzyme activity is obtained; the wild type Tcur-lipase is mutated by using structure analysis and site-directed mutagenesis technology, and the Tcur-lipase mutants N211A and N211A / D256A are obtained; the Tcur-lipase mutant improves the degradation efficiency of PBAT, and has a good industrial application prospect.

The invention discloses a preparation method of brown coconut yoghurt and yoghurt. The preparation method comprises the following steps: adding lipase into coconut milk for enzymolysis; mixing milk with the coconut milk subjected to enzymolysis, and performing browning to obtain a base material; adding sugar, starch and citrus into the base material, and mixing to obtain a mixture; sterilizing the mixture; and fermenting the sterilized mixture to obtain the brown coconut yoghurt. Coconut milk after enzymolysis is mixed with milk, browning is carried out to a certain degree, fermentation is carried out, the taste of part of the coconut milk after fermentation is changed, after browning, the product is appropriate in fragrance, the scorch aroma is increased, the roasted coconut yogurt with the rich flavor is prepared, the yogurt is brighter in color and full in color, the bitter taste of the product is reduced, and the unpleasant fragrance of a plant-based product is improved; the taste is smooth, the texture is stable, the state is more uniform, the texture is more uniform, and no water is separated from a substrate.

The present invention relates to a method for obtaining a detergent composition comprising introducing (a) a variant of a parent lipase having at least 60% sequence identity with SEQ ID NO:2, having a substitution at a position D254 of the mature polypeptide corresponding to SEQ ID NO:2, and having lipase activity; and (b) an anionic surfactant, wherein the composition has increased stability compared to a corresponding composition containing the parent lipase.

This invention relates to the field of semiconductor cleaning technology and discloses a cleaning agent for semiconductor silicon wafers and its preparation method. This cleaning agent aims to solve the problem that traditional strong alkaline or phosphate-based wet electronic chemical cleaning agents easily corrode the silicon wafer surface, damage the crystal lattice, and thus affect electrical performance. This cleaning agent utilizes a multi-component synergistic effect: a surfactant system composed of alkyl glycosides and sophorolipids enhances particle wetting and stripping; lipase and silicon-pharmacoprotein precisely catalyze the removal of specific contaminants; sodium gluconate complexes metal ions; gelatin-polyacrylamide copolymer disperses contaminants to prevent secondary contamination; glycerol and trehalose maintain enzyme activity; and ultrapure water ensures the system meets the electronic-grade purity standards for wet electronic chemicals. Overall, it avoids strong alkaline corrosion, maintains the integrity of the silicon wafer lattice, and improves particle removal efficiency, meeting the high standards required for wet electronic chemical-based silicon wafer cleaning agents in advanced processes.

The invention provides a biological sewage treatment accelerant as well as a preparation method and application thereof, and belongs to the technical field of sewage treatment. According to the application, the fruit and vegetable fermentation liquor, the inorganic carbon source, the lipase, the protease, the amylase, the cellulase, the B vitamins, the cytochrome c, the coenzyme Q and the ethylene glycol are used as main components to prepare the accelerant, the accelerant is added into a biological sewage treatment reaction tank, and the biological sewage treatment effect is enhanced by increasing the microbial nitration reaction and denitrification reaction rate.

The utility model discloses a kind of esterification with stirring ripening device. The aforementioned stirring ripening device includes reaction kettle, detachably installed at the top of reaction kettle's stirring box, stirring mechanism installed on stirring box, fixedly installed at the bottom of reaction kettle's support and fixed mechanism being set in reaction kettle and being connected with stirring mechanism. The fixed mechanism includes connecting box, reaction box, magnet piece I and magnet piece II. The reaction box is filled with macroporous adsorption resin, and lipase is adsorbed and fixed thereon. The utility model constructs composite stirring system, can produce multi-directional shear force, so that liquor is fully stirred and mixed, improves liquor ripening effect, in addition, has the adsorption and fixation function of lipase, avoids lipase loss with liquor, realizes reuse, reduces ripening cost.

The invention discloses a bacterium-enzyme compound feed additive and a preparation method thereof, and belongs to the field of compound functional feed additives, the bacterium-enzyme compound feed additive comprises the following raw materials in parts by weight: 10-30 parts of compound probiotics, 10-30 parts of a compound enzyme preparation, 20-30 parts of a protein-based microcapsule coating material, 5-10 parts of thyme essential oil and 3-8 parts of prebiotics. Wherein the compound probiotics comprise bifidobacterium, lactobacillus and bacillus subtilis according to the mass ratio of 1: (0.3 to 0.7): (0.3 to 0.7); the compound enzyme preparation comprises at least two of cellulase, beta-mannase, lactase, protease and lipase. By adding the bacterium-enzyme composite feed additive prepared by the invention, the proportion of beneficial bacteria in intestinal tracts and the absorption and utilization rate of feed nutrients can be improved.

This invention discloses a lipase mutant, an immobilized enzyme, and their applications, belonging to the field of bioengineering. The SpL enzyme mutant K193R / I134V described in this invention exhibits a specific activity of 7-ACA that is 1.6 times higher than before the mutation, and its half-life at 45℃, 50℃, and 55℃ is increased by 1.9 times, 2.6 times, and 3.8 times, respectively. Immobilizing the his-tagged SpL enzyme on a hydrophobically modified nickel-doped SBA-15 mesoporous molecular sieve preserves the enzyme's activity and improves its thermal stability and reusability. Using this immobilized enzyme to catalyze the production of D-7-ACA allows for at least 10 reusable batches, maintaining a conversion rate above 99%.

The invention relates to a fermentation enzymolysis technology, in particular to a production method for optimizing natural cheese essence by utilizing an electric field auxiliary technology. The method comprises the following steps: preheating milk, adjusting the pH value, adding trypsin and lipase, and uniformly mixing; and putting the obtained milk containing the enzyme into an electric field assisted reactor, carrying out electric field assisted fermentation enzymolysis under a stirring condition, and carrying out post-treatment on the obtained reaction product to obtain the cheese essence. By combining the electric field technology and the enzymolysis process, the reaction efficiency and the product quality are remarkably improved.

The application provides a method for synthesizing benzimidazole Michael addition derivatives in a continuous flow reactor, and 2-methylbenzimidazole and alpha, beta-unsaturated olefins are used to catalyze the online synthesis of benzimidazole Michael addition derivatives in a microfluidic channel reactor by using a lipase Lipozyme RM IM. The method not only greatly shortens the reaction time, but also has high conversion rate; meanwhile, the Michael addition reaction of 2-methylbenzimidazole and alpha, beta-unsaturated olefins is catalyzed by using the economical lipase Lipozyme RM IM in methanol, the reaction cost is reduced, the post-processing step is simplified, and the method has the advantages of green environmental protection, economy and efficiency.

Provided is a lipase mutant with alleviated inhibition of activity against a dispersed substrate in the presence of a surfactant. The lipase mutant consists of an amino acid sequence having an identity of at least 75% with the amino acid sequence of SEQ ID NO: 2, 4, 6, or 8 and has a predetermined amino acid residue at a predetermined position numbered according to SEQ ID NO: 2.

This invention discloses an enzyme-responsive silk fibroin nanovesicle, its preparation method, and its application, comprising the following steps: silk fibroin is mixed with a hydrophobic acylation reagent containing enzymatically cleavable chemical bonds and a catalyst for a grafting reaction to obtain amphiphilic modified silk fibroin; the amphiphilic modified silk fibroin is mixed with hair care active ingredients in an aqueous phase, and self-assembly is induced to form nanovesicles encapsulating the active ingredients through ultrasonic treatment or high-pressure homogenization. This invention uses silk fibroin as the main body to provide a biocompatible framework and self-assembly basis. Hydrophobic groups are introduced through chemical modification, and these hydrophobic groups are connected to the silk fibroin backbone through enzymatically cleavable chemical bonds. These chemical sites can be recognized and cleaved by sebaceous lipase secreted by the scalp sebaceous glands or other specific lipases highly expressed in the hair follicle microenvironment, achieving specific enzymatic release of the nanovesicles in the hair follicle microenvironment.

The present disclosure relates to methods, cells, and compositions for producing a product of interest, e.g., a recombinant protein. In particular, the present disclosure provides improved mammalian cells expressing the product of interest, where the cells (e.g., Chinese Hamster Ovary (CHO) cells) have reduced or eliminated activity, e.g., expression, of certain host cell proteins, e.g., enzymes including, but not limited to, certain lipases, esterases, and / or hydrolases.

The invention relates to lipid removal enhancers that may be used alone or in concert with lipase in detergents for removal of lipid stains, such as fats and oils, from surfaces, such as textiles, with an increased benefit-risk ratio.

This invention discloses a paint remover for steel-aluminum composite materials, belonging to the field of metal material surface treatment technology. It comprises the following components in weight percentages: 5%-8% alkaline substance; 5%-10% lipase component; 10%-16% solvent; 5%-10% surfactant; 6%-13% co-solvent; 5%-8% corrosion inhibitor; and water as the balance. The paint remover is prepared from alkaline substance, lipase component, solvent, surfactant, co-solvent, corrosion inhibitor, and water. It is used for removing paint from steel-aluminum composite materials. On one hand, the alkaline substance saponifies some groups in the paint, dissolving them in the solvent and water. On the other hand, heating and steaming the paint film reduces its strength and adhesion to the metal. Then, utilizing the wetting, penetration, and affinity effects of the surfactant, the catalytic effect of the lipase component, and the protective effect of the corrosion inhibitor on the steel-aluminum substrate, the goal of removing paint quickly without corroding the substrate is achieved.

The invention discloses a biodegradable polyester composition with controllable degradation time and a preparation method of the biodegradable polyester composition, and relates to the technical field of biodegradable materials. 20-40 parts by weight of carboxyl-terminated polylactic acid; 0.5 to 3.0 parts by weight of a bifunctional chain extender containing an acetal bond; 5 to 15 parts by weight of a polyethylene glycol-b-polycaprolactone block copolymer; 1-5 parts by weight of modified cellulose nanocrystals; and 0.1-0.5 part by weight of metal organic framework loaded lipase. By reasonably matching the components and optimizing the preparation process, the degradation time of the biodegradable polyester composition can be accurately controlled, and the degradation induction period and the complete degradation time can be adjusted as required. The compatibility of the components is good, the mechanical property and the processing stability of the material can be effectively improved, and the problem of inactivation or uneven dispersion of the components in the processing process is avoided.