25 results about "Serratia" patented technology

The invention provides an organic carrier in-root-rhizosphere growth-promoting microbial agent for ecological restoration of degraded grassland as well as a preparation method and application of the microbial agent, and belongs to the technical field of grassland ecological restoration and microbial agents. The organic carrier growth-promoting microbial agent disclosed by the invention is prepared from four kinds of growth-promoting bacteria (PGPE) in roots, four kinds of growth-promoting bacteria (PGPR) in roots and an organic carrier, the PGPE comprises terracotta warrior bacillus K4, bacillus 9-3-1, bacillus deserticola 11-5-4 and halomonas L31, the PGPR comprises serratia rubra 5, serratia rubra 23, cosinia cosinii 11 and enterobacter parabarium 24, and the organic carrier is a carbon-based organic fertilizer, a cattle and sheep manure organic fertilizer or biochar. When the organic carrier growth-promoting microbial agent is applied to the degraded grassland, the biomass and coverage of plant communities can be remarkably improved. The organic carrier growth-promoting microbial agent can be used for ecological restoration of degraded grassland.

The invention belongs to the field of development and application of microorganisms, and relates to serratia rubra for preventing and treating potato scab and application of the serratia rubra. In order to overcome the defects in the prior art, the invention provides serratia rubra A14 for preventing and treating potato scab and application of the serratia rubra A14, the serratia rubra A14 provided by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36010. The serratia rubra A14 can be used for preventing and treating potato scab. The strain has remarkable bacteriostatic activity on streptomyces scabies, and has the capabilities of dissolving phosphorus and producing indoleacetic acid (IAA), protease and siderophore. The strain has good biological safety, and the strain is prepared into a microbial agent for green prevention and control of potato scab and has good application prospects.

The present application relates to the field of microbial technology, and more particularly to a kind of for degrading high-efficiency fluazifop-butyl ester urea serratia DHPM01 and its application, urea serratia DHPM01 is preserved in China Microbial Culture Collection Center on September 18, 2025, preservation number is CGMCC NO.35971, and the address of preservation is Beijing Chaoyang District Beichen West Road No.1, and the classification designation is Serratia ureilytica The microbial agent provided by the present application can effectively degrade high-efficiency fluazifop-butyl ester in a wide pH and temperature range, and urea serratia DHPM01 can be used as an excellent pesticide-degrading bacterium and widely applied to the bioremediation of high-efficiency fluazifop-butyl ester residues in water, farmland and contaminated soil and other environments, providing a new efficient and environmentally friendly strategy for pesticide pollution control and enriching the germplasm resource bank of pesticide-degrading functional microorganisms.

This invention discloses the application of α-bisabolol in the preparation of a quorum sensing quencher for Serratia liquefaction. It belongs to the field of pharmaceutical technology. The purpose of this invention is to provide an application in the preparation of a quencher for inhibiting the quorum sensing system of Serratia liquefaction, thereby reducing the toxicity of pathogenic bacteria without inhibiting their growth. This invention provides an application of bisabolol in the preparation of a quencher for inhibiting the quorum sensing system of Serratia liquefaction. It offers a novel solution for developing efficient and safe food preservatives.

The invention relates to the technical field of protoplast preparation, in particular to a preparation method of protoplast of sclerotium rolfsii. The method comprises the following steps: carrying out enzymolysis on sclerotium rolfsii by adopting protoplast enzymatic hydrolysate; a Miracloth filter membrane is adopted for filtering, a container for collecting filtrate is placed on ice, and a precooled 0.6 M sorbitol solution is used for flushing; centrifuging the collected filtrate, collecting precipitates, cleaning the precipitates by using a precooled 0.6 M sorbitol solution to remove enzymatic hydrolysate, and then carrying out resuspension centrifugation by using STC to collect precipitates so as to obtain protoplasts; according to the characteristics of sclerotium rolfsii, a corresponding enzymolysis system is established, the enzymolysis condition is mild and easy to control, the obtained protoplast is complete and stable in form and high in yield, and the quantity of the protoplast reaches up to 1 * 10 < 8 > / mL.

The invention provides a phosphorus-solubilizing synthetic microbiome as well as a preparation method and application thereof, and particularly belongs to the technical field of synthetic microbiome. The phosphorus-solubilizing synthesis microbiome comprises the following microorganisms: bacillus subtilis with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.36218, bacillus subtilis with the preservation number of CGMCC No.36218, bacillus subtilis with the preservation number of CGMCC No.36218, bacillus subtilis with The preservation number of the serratia marcescens is CGMCC (China General Microbiological Culture Collection Center) No.36217, and the preservation number of the serratia marcescens is CGMCC No.36217. And Shigella flexneri, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.36219, and the preservation number of the Shigella flexneri is CGMCC No.36219. The phosphorus-solubilizing synthetic microbiome has a phosphorus-solubilizing effect, can realize the improvement of the simulated lunar soil, particularly can be used for dissolving insoluble phosphorus in the simulated lunar soil, and can promote the growth of crops in the simulated lunar soil.

The application discloses a new use of an agricultural enzyme and a method for controlling and inhibiting soil pathogenic bacteria biological pollution, wherein the agricultural enzyme is used as a soil repair agent to prevent or treat soil pathogenic bacteria biological pollution, and the soil pathogenic bacteria are at least one of Klebsiella pneumoniae, Acidovorax xylosoxidans, Streptomyces orientatis, Vibrio cholerae, Riemerella anatipestifer, Clostridium botulinum, Serratia marcescans, Rhodococcus globerulus and Aeromonas salmonicida. The agricultural enzyme is applied to the pathogenic bacteria biological pollution soil to be treated in different ways. The application directly kills the pathogenic bacteria or regulates the soil microbial community through a quorum sensing gene and a virulence factor gene, so that the abundance of the pathogenic bacteria in the soil community is reduced, and the soil pathogenic bacteria biological pollution is reduced. The application is an economic, green, convenient, efficient agricultural soil environment repair technology.

The invention discloses a multi-bacteria enzyme composition for water purification treatment and application, and relates to the technical field of water purification. The microbial agent is prepared from the following components in parts by weight: 15 to 30 parts of rhodopseudomonas palustris, 15 to 30 parts of bacillus subtilis, 25 to 35 parts of serratia marcescens, 25 to 35 parts of denitrifying pseudomonas, 25 to 35 parts of lactic acid coccus, 25 to 35 parts of bacillus amyloliquefaciens and 25 to 35 parts of rhodotorula benthica. The synergistic effect of the flora can inhibit the outbreak of harmful algae, promote the growth of beneficial algae, improve the flocculation performance of a water body and effectively improve the transparency. Meanwhile, the composition has a remarkable removal effect on key pollution indexes such as ammonia nitrogen, COD (Chemical Oxygen Demand), BOD (Biochemical Oxygen Demand), suspended solids and total phosphorus, so that the systematic repair of the water body is realized.

The invention belongs to the technical field of microorganism application, and particularly relates to a preparation method of a serratia rubra solid preparation for antagonizing pear postharvest penicilliosis. Firstly, serratia rubra B11 bacterial suspension is prepared and then evenly mixed with a composite protective agent of a specific formula, and the protective agent comprises four core components including trehalose, skim milk powder, L-sodium glutamate and xylooligosaccharide. And carrying out deep pre-freezing treatment on the mixed solution, and carrying out vacuum freeze drying under the condition of strictly controlling parameters to finally obtain the stable solid preparation. Experiments prove that the microbial inoculum prepared by the method can maintain higher biological activity during low-temperature storage, and the survival rate of thalli still exceeds 60% after the microbial inoculum is stored for 90 days under proper conditions. The preparation has a remarkable inhibition effect on diseases caused by penicillium expansum during the storage period of pears, and an effective and stable novel biological prevention and control product is provided for green prevention and control of postharvest diseases of fruits and vegetables.

The invention discloses a mixed microbial agent and application thereof in codonopsis pilosula planting. The composite flora is composed of phosphate solubilizing bacteria, phosphate solubilizing bacteria, phosphate solubilizing bacteria, phosphate solubilizing bacteria, phosphate solubilizing bacteria, phosphate solubilizing bacteria and phosphate solubilizing bacteria, potassium bacteria: pseudomonas aeruginosa and bacillus circulans; and nitrogen-fixing bacteria: autotrophic flavobacterium, clostridium pasteurianum and bradyrhizobium. Wherein the phosphate-solubilizing bacteria are wrapped by adopting a benzene diboronic acid-sodium alginate pH response material, the potassium-solubilizing bacteria are wrapped by adopting selenized sodium alginate-Ca < 2 + > salt ion response gel, and attapulgite is used as a carrier to form the slow-release bacterial agent. Phosphorus-solubilizing bacteria and potassium-solubilizing bacteria have a synergistic effect to maintain the optimal growth environment of codonopsis pilosula, trichoderma harzianum colonization is promoted through bacillus circulans exopolysaccharide to construct a synergistic network, and the acting effect of the fungicide is improved by combining dual-environment response release and boron-selenium element synergy. During application, the microbial inoculum and rotten stems and leaves of codonopsis pilosula are fermented to prepare a bacterial fertilizer for basal application, and then topdressing and flushing application are performed, so that the yield and polysaccharide content of codonopsis pilosula are remarkably increased.

The invention belongs to the technical field of grassland restoration, and relates to a microbial ecological restoration method for a degenerated typical grassland, which comprises the following steps: selecting the degenerated typical grassland to be treated, ditching, applying a carrier growth-promoting microbial agent, and combining the carrier growth-promoting microbial agent with an arbuscular mycorrhizal fungus agent and / or an azotobacter agent according to the degeneration type. The carrier growth-promoting microbial agent comprises specific strains such as serratia rubra, enterobacter paraphora, cosinia cosinii, bacillus and halomonas. The carrier growth-promoting microbial agent is prepared from the specific strains such as serratia rubra, enterobacter paraphora, cosinia cosinii, bacillus and halomonas. The microbial inoculum is cultured and prepared by being loaded on an organic fertilizer carrier, and the application amount is adjusted according to different degeneration degrees. According to the method, vegetation recovery is remarkably promoted through the synergistic effect of three main microbial functional groups of soil, the biomass and coverage of plant communities are improved, soil nutrients and microbial activity are improved, and the method is suitable for various degeneration types such as grazing degeneration, cradling degeneration and desertification typical grassland; the method has the advantages of being good in remediation effect, environmentally friendly, high in sustainability and the like.

The application discloses a Serratia plymuthica strain for preventing and treating soybean root rot and application thereof. A Serratia plymuthica strain G25-16 is isolated from the rhizosphere soil of soybean and wheat, and the microbial preservation number of the strain is CGMCC No.34772; the results of a plate confrontation experiment show that the strain has obvious antagonistic effects on Fusarium solani and Fusarium retusum; the results of a prevention and treatment experiment show that the strain has significant prevention and treatment effects on the Fusarium solani and the Fusarium retusum; and the results of a pot planting growth promotion experiment show that after the soybean is treated by the isolated Serratia plymuthica strain, the fresh weight and the plant height of the soybean are obviously improved compared with a control. The isolated Serratia plymuthica strain G25-16 has application prospects in promoting the growth of soybean and preventing and treating soybean diseases.

The invention relates to the technical field of microorganisms, in particular to serratia urealyticum DHPM01 for degrading haloxyfop-R-methyl and application of the serratia urealyticum DHPM01. The serratia urealyticum DHPM01 is preserved in the China General Microbiological Culture Collection Center on September 18, 2025, the preservation number is CGMCC NO.35971, the preservation address is No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.35971. The serratia urealyticum DHPM01 is CGMCC NO.35971. The method comprises the following steps: classifying and naming as Serratia ureilytica; the microbial agent for degrading haloxyfop-R-methyl provided by the invention can effectively degrade haloxyfop-R-methyl in a wide pH and temperature range, serratia urealyticum DHPM01 can be used as an excellent pesticide degrading bacterium, and is widely applied to bioremediation of haloxyfop-R-methyl residues in environments such as water, farmland and polluted soil; a new efficient and environment-friendly strategy is provided for pesticide pollution treatment, and a germplasm resource library of microorganisms with pesticide degradation functions is enriched.

The invention belongs to the technical crossing field of microbiomics, and relates to a method for constructing a steady-state microbiome on the surface of a pear based on serratia rubra to inhibit postharvest diseases. The method comprises the following steps: firstly, preparing serratia rubra B11 suspension, and spraying the serratia rubra B11 suspension on the surfaces of pear fruits; the method comprises the following steps: standardly collecting epiphytic microorganisms on the surfaces of fruits by adopting a mode of combining physical elution and vacuum filtration, extracting total DNA, respectively carrying out high-throughput amplicon sequencing on a 16S rRNA gene V3-V4 region and a fungus ITS2 region, and carrying out dynamic analysis on alpha diversity, beta diversity, community composition and key class groups, so as to determine the content of the epiphytic microorganisms on the surfaces of the fruits. And finally, associating with the fruit rotting rate and the physiological quality index, establishing a set of full-process standardized system of multi-dimensional bioinformatics analysis, and revealing an ecological mechanism that beneficial bacteria realize disease inhibition by remodeling a fruit surface microbial community structure and regulating and controlling key function class groups. And reliable technical support is provided for mechanism research, efficiency evaluation and application development of the biocontrol microbial inoculum.

This invention discloses the application of isohypericin in the preparation of a quencher for inhibiting the quorum sensing system of *Serratia liquefaction*. It belongs to the field of pharmaceutical technology. The purpose of this invention is to provide a quencher for inhibiting the quorum sensing system of *Serratia liquefaction*, thereby reducing the toxicity of pathogens without inhibiting their growth. This invention provides the application of isohypericin in the preparation of a quencher for inhibiting the quorum sensing system of *Serratia liquefaction*. It offers a novel solution for developing highly efficient and safe food preservatives.

The invention discloses an application of atractylenolide III in preparation of a serratia liquefaciens quorum sensing quenching agent. Belongs to the technical field of medicine. The invention aims to provide the medicine for inhibiting the bacterial quorum sensing system of serratia liquefaciens, so that the toxicity of pathogenic bacteria is weakened on the premise of not inhibiting the growth of the pathogenic bacteria. The invention provides an application of atractylenolide III in a quenching agent for inhibiting a serratia liquefaciens quorum sensing system. A brand new solution is provided for developing an efficient and safe food biological preservative.

The application belongs to the polymer field and discloses application of a nanocellulose / chitosan active packaging film in preservation of Russula, wherein the nanocellulose / chitosan active packaging film is prepared by mixing Ag@MOF carriers loaded with natural antibacterial agent natal oil and nanocellulose / chitosan compound, and the obtained product has good tensile strength, elongation at break and water vapor permeability, and shows good film ductility and gas barrier performance; the packaging film is applied to Russula packaging, can effectively inhibit the growth of specific spoilage bacteria such as Pseudomonas, Serratia and Penicillium during the storage of Russula, so as to achieve the purpose of preserving Russula, and can slowly release the natal oil, thereby prolonging the preservation period of Russula.

Disclosed are compositions comprising poly(hexamethylene) biguanide (PHMB) or a pharmaceutically or veterinary acceptable salt thereof, a chelating agent, and a buffering agent and their use for disrupting and eradicating biofilms. The invention also relates to the use of the compositions for treating or inhibiting the development of an infection caused by a bacterium selected from the group consisting of Burkholderia spp., Serratia spp., Enterococcus spp. and Corynebacterium spp. and / or increasing the susceptibility of such bacterial strains to antibiotics.

The invention provides a high-yield prodigiosin method, a prodigiosin protection method and application. The method comprises the following steps: obtaining a serratia nematophagi mutant strain, screening serratia nematophagi with high and stable yield from the mutant strain, carrying out fermentation culture, and separating prodigiosin from a fermentation culture product, the serratia nematophila is serratia nematophila, the strain number of the serratia nematophila is S207, the serratia nematophila is preserved in the China Center for Type Culture Collection, and the preservation number of the serratia nematophila is CCTCC NO: M 2025727. The protection method of the prodigiosin comprises the following steps: storing the prodigiosin in a protective agent with the pH value of 2-6 at 4-60 DEG C, and avoiding the irradiation of ultraviolet light. A large amount of prodigiosin can be rapidly produced by using the method for producing the prodigiosin at high yield, the prodigiosin can be stably preserved by using the protection method for the prodigiosin, the decoloration rate of the prodigiosin can be reduced, and the growth and reproduction of bacteria and fungi can be effectively inhibited by using the bacteriostasis method for bacteriostasis.

The invention discloses an antibacterial pediococcus pentosaceus cell-free supernatant and an application of the antibacterial pediococcus pentosaceus cell-free supernatant in pork preservation, and belongs to the field of food preservation, the pediococcus pentosaceus is pediococcus pentosaceus YZJC001 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33185. The invention further discloses a preparation method of the antibacterial pediococcus pentosaceus cell-free supernatant and an application of the antibacterial pediococcus pentosaceus cell-free supernatant. Compared with other lactic acid bacteria, the cell-free supernatant of the pediococcus pentosaceus has the advantages that the growth of serratia, salmonella typhimurium, staphylococcus aureus and listeria monocytogenes can be obviously inhibited, and the quality guarantee period of the pork can be prolonged after the fresh pork is treated.

The invention relates to a bioactivating composition for providing phosphorus that is readily available to plant crops, comprising i) a combination comprising: (a) an organic waste source as a phosphorus-rich carrier matrix composed of excreta stabilised by means of aerobic composting; (b) enzymes that mineralise phytate; (c) a bacterial consortium that solubilises phosphorus, comprising bacteria from the groups Klebsiella, Stenotrophomonas, Serratia, and (ii) a polymer for homogenising and granulating the composition. The invention also relates to the method for obtaining said bioactivating composition and the use thereof.

The application provides a kind of medicine composition for regulating blood pressure and blood fat and its preparation method, belongs to the technical field of biopharmaceutical, and the medicine composition includes the carved shell extraction peptide, white ground mold intracellular extract, salvianolic acid B, total alkaloids of Uncaria and hawthorn total flavone.The carved shell extraction peptide is the product of fresh meat of carved shell after being crushed by alkaline protease, serratia peptidase and trypsin.The white ground mold intracellular extract is the product obtained by the following steps: ultrasonic disintegration of mycelium of white ground mold in ethanol aqueous solution, reflux extraction, centrifugation, removal of ethanol, purification by AB-8 weak polar macroporous adsorption resin, elution with ethanol aqueous solution, and removal of ethanol.The components are used in a certain proportion, which can simultaneously improve the vascular sclerosis damage caused by abnormal blood pressure and blood fat, and regulate blood pressure and blood fat index and maintain the blood vessels.

The invention belongs to the technical field of biology, and particularly relates to a microbial marker combination for evaluating the genuine nature of polygonatum sibiricum and application, the microbial marker combination is composed of Escherichia. Shigilla, Acremonium, Pseudomonas, Serratia, Mortierella, Wallemia, Engydonium, Sporidesmium, Ceratobasidium, Clonostachys and Ralstachys, an evaluation method for the genuine nature of polygonatum sibiricum is established, the sensitivity is 91.7%, the specificity is 100.0%, the AUC is 93.8%, and the method has the advantages of being high in sensitivity, strong in specificity and high in accuracy and can be used for evaluating the genuine nature of polygonatum sibiricum. The method can be used for judging whether the production area of the polygonatum sibiricum is a genuine Suhua production area or not, and can be widely applied to genuine evaluation of the polygonatum sibiricum.

The invention discloses a system for predicting / assisting in predicting prognosis of laryngeal squamous cell carcinoma based on a microbial marker, which associates recurrence of laryngeal squamous cell carcinoma with a microbial community for the first time, integrates a cancer-promoting microorganism fusobacterium and a cancer-inhibiting microorganism Serratia, and can be used for predicting the prognosis of laryngeal squamous cell carcinoma, so that the recurrence of laryngeal squamous cell carcinoma and the recurrence of laryngeal squamous cell carcinoma can be predicted and the prognosis of laryngeal squamous cell carcinoma can be predicted. According to the method, the content of microorganisms is detected on the basis of quantitative PCR, risk score assignment is performed according to-delta Ct obtained by a cycle threshold Ct of the microorganisms, and then the risk score is calculated according to a prognosis risk scoring formula, so that the prognosis risk of the laryngeal squamous cell carcinoma, especially the recurrence risk, can be classified according to a calculation result; the method can provide a new direction for prognosis prediction of laryngeal squamous cell carcinoma, especially for prediction of recurrence risk and disease-free survival rate within 5 years.

The application belongs to the technical field of biology, and specifically discloses a Serratia plymuthica R72, which is preserved in the China Center for Type Culture Collection, and the preservation date is February 2, 2026, and the preservation number is CCTCC M 2026311; can be applied to the prevention and treatment of atractylodes rhizome root rot, and the prevention and treatment effect on the main pathogenic bacteria of atractylodes rhizome root rot, i.e., Fusarium oxysporum, is 89.3% in a plate confrontation test; meanwhile, the Serratia plymuthica R72 provided by the application has strong antagonistic effects on the pathogenic bacteria of rice false smut, sclerotinia sclerotiorum, sclerotinia sclerotiorum, tomato gray mold, melon fruit rot, brassica nigra, rice blast, rice sesame leaf spot, and tomato wilt, and the inhibition rate is more than 88%.