79 results about "Serratia" patented technology

The present invention provides a novel class of monoclonal antibodies which have a high affinity, broad spectrum neutralizing reactivity to flagellin from various Gram-negative bacteria including, but not limited to, E. coli, Salmonella, Serratia, Proteus, Enterobacter, Citrobacter, Campylobacter and Pseudomonas. The present invention further provides methods of treating infections and diseases using anti-flagellin antibodies in humans, other animals and birds.

An immobilized microbial consortium is formulated which comprises of a synergistic mixture of isolated bacteria namely, Aeromonas hydrophila, Pseudomonas aeruginosa, Yersinia enterocolitica, Serratia liquefaciens, Pseudomonas fluoresces, Enterobacter cloaca, Klebsiella oxytoca, Citrobacter amalonaticus and Enterobacter sakazaki. The formulated microbial consortium is immobilized on charged nylon membrane. The said immobilized microbial consortium is attached to dissolved oxygen probe for the preparation of electrode assembly. The prepared electrode assembly is used for rapid and reliable BOD estimation. The prepared electrode assembly is used for monitoring of BOD load of synthetic samples such as Glucose-Glutamic acid (GGA) used as a reference standard in BOD analysis and industrial effluents; covering a range from low to high biodegradable organic matter.

The invention discloses a novel compound microorganism fertilizer. The novel compound microorganism fertilizer is prepared from the following raw materials in parts by weight: 50 to 70 parts of an organic fertilizer, 20 to 30 parts of an inorganic fertilizer, 5 to 10 parts of a regulator and 3 to 5 parts of microorganism bacterial powder, wherein the microorganism bacterial powder is prepared from the following raw materials in parts by weight: 5 to 10 parts of enterobacter cloacae, 5 to 8 parts of serratia carollera phosphaticum, 3 to 5 parts of bacillus mucilaginosus and 2 to 3 parts of rhodospirillum fulvum. The novel compound microorganism fertilizer is prepared through the following steps: adding the enterobacter cloacae, the serratia carollera phosphaticum, the bacillus mucilaginosus and the rhodospirillum fulvum into fermentation equipment for fermenting respectively; after adsorbing fermented bacterium liquid, mixing and crushing to obtain the compound microorganism bacterial powder; mixing, crushing, granulating, drying, enveloping and packaging the organic fertilizer, the inorganic fertilizer, the regulator and the microorganism bacterial powder. The novel compound microorganism fertilizer has good economic benefits, social benefits and ecological benefits.

The invention discloses an alpine grassland pasture endogenous serratia plymuthica strain GH010 capable of inhibiting various pathogenic bacteria and discloses a microbial agent prepared from the alpine grassland pasture endogenous serratia plymuthica strain GH010 and a preparation method thereof. The alpine grassland pasture endogenous serratia plymuthica strain GH010 and the microbial agent prepared from the same disclosed by the invention have the beneficial effects of having good inhibition effects on the various pathogenic bacteria; the inhibition effect is 67.5% of chili rhizoctonia solani and 69.5% of rape sclerotinia sclerotiorum; the bacteriostasis rates of cotton rhizoctonia solani, eggplant sclerotinia sclerotiorum, tomato botrytis cinerea, fusarium wilt of cucumber, exserohilum turcicum, bipolaris maydis or bipolaristriticola are more than or equal to 57.5%; and the strain has growth-promoting effects on crops including alfalfa and the like, and the environmental protection properties of a microbial pesticide and a microbial fertilizer prepared from the strain are good, the resistance to drugs is unlikely to generate and the safety is good.

The invention discloses a preparation method of a microbial flora preparation and application thereof. The preparation method comprises the steps of fermenting five agricultural product processing wastes such as onion processing wastes, soybean processing wastes, rice bran, wheat bran and abandoned fig at 37 DEG C to prepare mother bacteria yeast powder, the obtained yeast powder contains pediococcus acidilactici, lactobacillus plantarum, lactobacillus pentosus, lactobacillus paracasei, agrobacterium tumefaciens, Serratia, lactobacillus fermenti, agrobacterium, iron base autotrophic nitrifying bacteria, cucumber stem xanthomonas ochrobactrum anthropi, lactobacillus casei, nasturtium fructose bacillus, bacillus aceticus and the like. The yeast powder can be put into bags for long-term preservation after being dried. The yeast powder, molasses, red ginseng tails and water are stirred and cultured for 72 hours by introducing oxygen to prepare a liquid compound bacteria preparation, and the liquid bacteria preparation can be preserved for one year under room-temperature and shady and cool conditions. The microbial flora preparation can be used when attenuation by water before being used, and the effects on aspects of cultivation, compost treatment, wastewater treatment, soil improvement and the like are obvious.

The invention discloses tolerant serratia grimesii for enriching heavy metals and an application of the serratia grimesii. Bacterium is subjected to 16S rRNA gene sequence analysis to obtain a gene nucleotide sequence of the 16S rRNA as shown in SEQ ID NO:1 in a sequence table. By homology analysis, the strain is proven to belong to the serratia grimesii, named as DJ16 and preserved in China Typical Culture Collection Center on 19th Nov, 2014, and the preservation number is CCTCC NO: M 2014578. The serratia grimesii DJ16 disclosed by the invention can be used for enriching the heavy metals in soil and water.

The invention relates, in part, to nucleic acid constructs, genetically modified host cells and methods employing such constructs and host cells to increase the production of 3-methyl-2-butenol from IPP. Thus, in some aspects, the invention provides a genetically modified host cell transformed with a nucleic acid construct encoding a fusion protein comprising a phosphatase capable of catalyzing the dephosphorylation of dimethylallyl diphosphate (DMAPP) linked to an IPP isomerase capable of converting IPP to DMAPP, wherein the nucleic acid construct is operably linked to a promoter. In some embodiments, the genetically modified host cell 5 further comprises a nucleic acid encoding a reductase that is capable of converting 3-methyl-2-butenol to 3-methyl-butanol. In some embodiments, the reductase is encoded by a nucleic acid construct introduced into the cell. In some embodiments, the IPP isomerase is a Type I isomerase. In some embodiments, the IPP isomerase is a Type II isomerase. In some embodiments, the host cell is selected from a group of taxonimcal classes consisting of 20 Escherichia, Enterobacter, Azotobacter, Erwinia, Bacillus, Pseudomonas, Klebsiella, Proteus, Salmonella, Serratia, Shigella, Rhizobia, Vitreoscilla, Synechococcus, Synechocystis, and Paracoccus taxonomical classes. In some embodiments, the host cell is an Escherichia coli cell. In some embodiments, the host cell is a fungal cell, such as a yeast cell. In some embodiments, the yeast cell is a Saccharomyces sp. cell. In some embodiments, the host cell is an algal, insect or mammalian cell line. In some embodiments, the phosphatase is nudB from E. coli. In some embodiments, the IPP isomerase is encoded by an idi gene from E. coli or idil gene from Saccharomyces cerevisiae.

The invention relates to a microbial fermentation functional organic compound fertilizer and a production method thereof. The method comprises the following steps of: mixing lactobacillus buchneri (i) Lactobacillusbuchneri ( / i) CICC (China Center of Industrial Culture Collection) 6004, bacillus cereus (i) Bacilluscereus ( / i) ACCC (Agricultural Culture Collection of China) 10117, saccharomycescerevisiae (i) Saccharomyces ( / i) (i) cerevisiae ( / i) CICC1905, and serratia marcescent (i) Serratia ( / i) (i) marcescent ( / i) ACCC10119; and fermenting to produce an bio-organic fertilizer. With the adoption of the mixing-fermenting method, the estrogen and medicine residues in excrements of livestocks can be effectively degraded; nematode and fly maggot in the soil can also be eliminated; in addition, the shelf life of the organic fertilizer and the survival time of viable organism can be greatly prolonged, and the germination rate and stress resistance of crops seeds can also be improved, etc.

A method of treating or preventing diabetes or obesity that comprises administering an antioxidant-enriched composition produced by fermenting fruit extracts with the bacteria Serratia vaccinii. The antioxidant-enriched composition is capable of decreasing plasma glucose levels, increasing circulating adiponectin levels and decreasing hyperphagia in a subject.

The invention belongs to the fields of microbial application technology and biological restoration of contaminated soil, and particularly relates to a Serratiasp. strain and application thereof in the restoration of salinized petroleum-contaminated soil. The Serratiasp. strain disclosed by the invention was preserved by China General Microbiological Culture Collection Center on September 9th, 2011, and the preservation number is CGMCC NO.5236; and the strain can efficiently degrade petroleum in a high-salt liquid environment and salinized soil. The strain is separated out from salinized petroleum-contaminated soil under salt stress conditions. The strain has regular edges, and is round in colony, non-transparent and white in color; and the thallus is a short rhabditiform and gram negativeone having a small size. The invention also discloses a nucleotide sequence of the Serratiasp. strain. The strain can degrade petroleum in a high-salt liquid environment, has a petroleum degradation rate of 60% or above in salinized soil, and has an obvious application effect in the restoration of salinized soil in the field.

The invention provides a recombinant Escherichia coli, and a method for preparing phospholipase A1 through using the recombinant Escherichia coli. A gene-recombinant Escherichia coli BL21(DE3) / pET-pla CCTCC M 2012423 with the phospholipase A1 from liquefied Serratia liquefaciens CICC No.21538 is successfully constructed in the invention, and undergoes liquid fermentation to prepare hemolytic phospholipase A1, and the preparation method of the hemolytic phospholipase A1 comprises the steps of inclined plane culture, seed culture, autonomous induction culture (comprising permeable substance addition), and crude enzyme liquid extraction. The method for preparing the phospholipase A1 through the recombinant Escherichia coli has the advantages of high enzyme yield, high enzyme activity, simple production technology, short production period, low cost, easy technological amplification and the like, and can be widely applied to the grease refining field, the phosphatide modification field and the like.

The invention relates to a preparation method of a compound microbial flocculant for cyanobacterial bloom. The preparation method comprises the following steps: respectively inoculating Serratia and Bacillus into a sterilized nutrient broth medium for culture; respectively taking out 1mL of bacterial liquids of Serratia and Bacillus after culturing for 24h, and inoculating into a sterilized fermentation medium for fermentation culture; treating a broth as a seed liquid after culturing for 72 h and carrying out complex culture for 72h under conditions of a culture temperature of 35 DEG C, a rotation speed of 160rpm, an initial pH of a medium of 8.0, and an inoculation amount of the seed liquid of 10 ml / L; and carrying out centrifugal separation on the broth, and taking out a supernatant liquor. So the compound microbial flocculant is obtained. The preparation method is simple, fast and low cost, the flocculant prepared in the invention has the advantages of small dosage in use, strong stability and easy storage, can effectively flocculate cyanobactreial water samples with a flocculation rate of 92%, and has an excellent application prospect.

The invention provides a serratia strain with organic solvent tolerance, which is named Serratiamarcescens MH6, and has a preservation registration number of CCTCC M 208205. The invention also provides organic solvent tolerant protease produced by the strain, the organic solvent tolerant protease has an amino acid sequence shown in SEQ ID NO: 2, and an encoding gene of the organic solvent tolerant protease has a nucleotide sequence shown in SEQ ID NO: 1. The strain Serratiamarcescens MH6 is an extreme microorganism capable of tolerating organic solvents, can produce the organic solvent tolerant protease, and enrich the variety of extreme microorganisms and the sources of the organic solvent tolerant protease. The protease has good organic solvent tolerance compared with other natural proteases, and is expected to be applied to the synthesis of polypeptides in nonaqueous phases and the theoretical research related to a mechanism of organic solvent tolerance of the protease.

The invention relates to the field of microorganisms, in particular to an application of bacillus prodigiosus ITBB B5-1 to production of prodigiosins. The preservation No. of the bacillus prodigiosusis CGMCC No.7416. The prodigiosins are produced by the bacterial strain, the yield can achieve 9.8-15.5g in each liter of culture mediums, and the yield is notably higher than that of a bactria strainwhich is reported. The restriction of being low in yield of the prodigiosins by the bacillus prodigiosus bacterial strain is overcome, and the bacillus prodigiosus ITBB B5-1 has wide application prospects in the medicine markets.

The invention discloses a magnet generator, and a solid particle adsorption device and an air sterilization machine manufactured through the same, belongs to the technical field of air purifiers, and is creative. The magnet generator comprises seven layers, and flat high voltage direct current is introduced to the third layer and the fifth layer. The magnet generator can be called a full-material magnet generator. The solid particle adsorption device and the various air sterilization machines manufactured through the magnet generator are high in air purification efficiency and large in space, have the killing capacity for staphylococcus albus, natural bacteria in air, escherichia coli, staphylococcus aureus, candida albicans, serratia marcescens and the like in the air, can generate the hundred-million-level negative oxygen ion concentration, and can be widely applied to a sterile laboratory, a hospital, an operating room, a meeting room, a nursing home, a school, a factory workshop and the like. The work efficiency is greatly improved, and the profits are improved. Generated negative oxygen ions enable solid fine particles in the air to be electrified, adsorption with the magnet generator is facilitated, and a circle is formed. The magnet generator, and the solid particle adsorption device and the air sterilization machine manufactured through the same have wide market prospects.

The present invention aims at providing a vaccine composition capable of being administered to an intraoral mucous membrane, ocular mucous membrane, ear mucous membrane, genital mucous membrane, pharyngeal mucous membrane, respiratory tract mucous membrane, bronchial mucous membrane, pulmonary mucous membrane, gastric mucous membrane, enteric mucous membrane, or rectal mucous membrane, that is safe, useful as a prophylactic or therapeutic agent for infectious diseases or cancers, and capable of effectively inducing the systemic immune response and mucosal immune response. The present invention provides a mucosal vaccine composition to be administered to at least one mucous membrane selected from the group consisting of a human or animal intraoral mucous membrane, ocular mucous membrane, ear mucous membrane, genital mucous membrane, pharyngeal mucous membrane, respiratory tract mucous membrane, bronchial mucous membrane, pulmonary mucous membrane, gastric mucous membrane, enteric mucous membrane, and rectal mucous membrane, the mucosal vaccine composition containing: at least one antigen; and as an adjuvant, a lipopolysaccharide derived from at least one gram-negative bacterium selected from the group consisting of Serratia, Leclercia, Rahnella, Acidicaldus, Acidiphilium, Acidisphaera, Acidocella, Acidomonas, Asaia, Belnapia, Craurococcus, Gluconacetobacter, Gluconobacter, Kozakia, Leahibacter, Muricoccus, Neoasaia, Oleomonas, Paracraurococcus, Rhodopila, Roseococcus, Rubritepida, Saccharibacter, Stella, Swaminathania, Teichococcus, Zavarzinia, Pseudomonas, Achromobacter, Bacillus, Methanoculleus, Methanosarcina, Clostridium, Micrococcus, Flavobacterium, Pantoea, Acetobacter, Zymomonas, Xanthomonas, and Enterobacter, or a salt thereof, wherein a mass ratio between the adjuvant and the antigen (total mass of the adjuvant / total mass of the antigen) is 0.002 to 500.

The invention relates to a composite bacteria preparation for reducing ammonia release and preserving nitrogen in cow dung compost and belongs to the field of microorganisms. The invention aims at solving the problem that the existing biological preparation for reducing the volatile quantity of NH3 in the compost fermentation process through taking cow dungs as main raw materials and preserving the nitrogen is not invented. The composite bacteria preparation comprises the following components in part by weight: 12-20 parts of Serratiaplymuthica fermentation liquor with a collection number of ACCC No. 01503, 17-25 parts of Sphingomonastrueperi fermentation liquor with a collection number of ACCC No. 12417 and 50-63 parts of Alcaligenessp fermentation liquor with a collection number of DSM No. 2625. The composite bacteria preparation can greatly reduce the release amount of the NH3 in the composting process and effectively improve the content of nitrogen elements in a fertilizer.

Immobilized cell beads incorporating formulated microbial consortium comprising a synergistic mixture of the following bacterial strains namely, Enterobacter sakazaki, Pseudomonas aeruginosa and Aeromonas sobria selected from the following isolated bacterial strains namely, Yersinia enterocolitica, Aeromonas sobria, Klebsiella pneumoniae, Serratia liquefaciens, Enterobacter sakazaki, Citrobacter amalonaticus, Pseudomonas fluorescens, Pseudomonas aeruginosa, Enterobacter cloaca, Acinetobacter calcoaceticus are prepared, the formulated microbial consortium is immobilized in an appropriate immobilizing agent resulting in the formation of beads and the beads are used for instant BOD estimation using an electronic device and the formulated cell beads are reusable and are capable of assimilating most of the organic matter present in varied industrial effluents.

Biofungicide composition derived from a biologically pure culture of a Chilean bacterial isolate obtained from the skin of grapes, corresponding to Serratia plymuthica CCGG2742, to be used as an environmentally friendly biological control agent against fungal diseases of vegetables, in particular fruits susceptible to the infection of Botrytis cinerea, efficiently preventing the germination of conidia and the proliferation of mycelia of said phytopathogenic fungus, furthermore protecting the plant's leaves and fruits from the infection by the same fungus, and having the potential of being used in the biological control of other phytopathogenic fungus and microorganisms.

The invention relates to a serratia plymithica synthetic culture medium and a method for preparing fermentation liquid of the serratia plymithica synthetic culture medium. The formula (gram / liter) of the culture medium comprises the following components of 2 to 10 of colloidalchitin, 3 to 10 of beef extract, 5 to 15 of peptone, 6 to 10 of sodium chloride, 1 to 5 of ammonium sulfate, 1 to 3 of sodium citrate, 1 to 5 of dipotassium phosphate, 0.5 to 3 of magnesium sulfate and 0.01 to 0.05 of ferrous sulfate. The serratia plymithica high-yield fermentation liquid for producing a large quantity of active substances and chitinase which are used for suppressing aflatoxin can be obtained by continuously culturing on a table concentrator at the temperature within 28 and 35 DEG C and the speed of 140 r / min for 4 to 6 days according to the inoculation quantity within 1 to 10 percent. The activity of the chitinase in fermentation supernate is 2.04 to 5.36U / ml; the rate of suppressing the growth of parasitic aspergillus silk is 80.13 to 89.56 percent; and the rate of suppressing the aflatoxin is 90.22 to 98.31 percent. The culture medium is rational in component, and the preparation method is simple and reliable.

A method and composition for treatment of bacterial infections caused by gram negative or gram positive bacteria such as Staphylococcus aureus, Rhodococcus equi, Mycobacterium tuberculosis, Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Pseudomonas aeruginosa, Haemophilus influenzae, Proteus mirabilis, Enterobacter species, Serratia marcescens as well as those caused by Burkholderia cepacia, Stenotrophomonas maltophilia, Alcaligenes xylosoxidans, and multidrug resistant Pseudomonas aeruginosa, using a formulation containing gallium (III), in a pharmaceutically acceptable salt or complex thereof.

The invention discloses a microbial insecticide for preventing and controlling aphids. The microbial insecticide is obtained by activation culturing of solid strains. The strains include 22-28% of Pandora neoaphidis strain, 15-20% of acinetobacter baumannii, 12-18% of bacillus thuringiensis, 13-18% of bacillus amyloliquefaciens, 10-15% of bacillus subtilis, and 12-16% of serratia. The microbial insecticide has the beneficial effects that by using the symbiotic relationship of Pandora neoaphidis strain and acinetobacter baumannii and the mutual cooperative effects of acinetobacter baumannii, bacillus thuringiensis, bacillus amyloliquefaciens, bacillus subtilis and serratia, Pandora neoaphidis strain helps acinetobacter baumannii, bacillus thuringiensis, bacillus amyloliquefaciens, bacillussubtilis and the like to invading into cells of aphids, and finally the aphids are sick, so that the efficiency of killing insects is improved.

The invention discloses a method for preparing serratia nuclease through efficient secretory fusion expression and recombination in methylotrophic yeast. Hirudin is located at the N terminal of the fusion expression, nuclease is located at the C terminal of the fusion expression, the middle is a DDDDK sequence, and the specific structure is shown as follows: Hirudin-DDDDK-NUC. According to the method, the serratia nuclease with high yield, high specific activity and low cost is obtained by utilizing the closed activity of the hirudin and the high expression of the hirudin for the first time.

Compositions comprising biotransformed fruit extracts and methods of using the compositions in the prevention and treatment of cancer are provided. The fruit extracts are biotransformed by fermentation with a bacterial strain having all the identifying characteristics of Serratia vaccinii. The compositions can be used to inhibit tumour growth, tumour metastasis and cancer stem cell (CSC) development.

The invention discloses an antibacterial agent containing serratia-marcescens metabolite prodigiosin and a preparing method. A methanol solution of the prodigiosin is added into a glycolic acid watersolution through a polytetrafluoroethylene hollow fiber membrane under the pressure effect, and the prodigiosin is evenly dispersed in an ultrafine-particle manner to form suspension; then the suspension and pregelatinized starch dispersed in a waterborne system are subjected to mixing ball milling, and through the adhesive effect of the pregelatinized starch, it is further promoted that the prodigiosin stably exists in the water environment; finally, polyvinyl-alcohol hollow microspheres are introduced, high-pressure homogenization treatment is conducted, then freezing, heating and drying areconducted, rich porous structures are formed, and microorganisms including bacteria are attracted and gathered, and the prodigiosin can fully play the bactericidal effect. According to the antibacterial agent containing serratia-marcescens metabolite prodigiosin and the preparing method, a bactericide obtained with the method can play the excellent bactericidal effect in an aquaculture system.

A Serratia bacillus able to generate the stereoselective lipohydrolase features that after it is fermented and cultured, it can generate the lyolipase, which can be used to split the racemate (+ / -)-methylester of p-methoxyphenyl glycilic acid to obtain the (-)-methylester of p-methoxyphenyl glycilic acid, which is the key chiral precursor of diltiazem for treating cardiovascular diseases.

Physiologically acceptable anti-biofilm compositions comprising Serratia peptidase and optionally one or more of bromelain, papain and a fibrinolytic enzyme. Additional components can include antimicrobials, antibiotics, antifungals, herbals, chelating agents, lactoferrin and related compounds, minerals, surfactants, binders, and fillers useful for the inhibition and treatment of gastrointestinal biofilms in humans. Physiologically acceptable anti-biofilm compositions containing these enzymes are useful in the inhibition, reduction and / or treatment of biofilms such as in the ear, vagina, joints, bones, gut, surgical sites and other locations, and are useful for the inhibition, reduction and / or treatment of associated systemic symptoms caused by biofilm associated microorganisms.