82 results about "Broth medium" patented technology

The present invention relates to a composite microbial agent, a preparation method thereof and an application in the preprocessing of kitchen garbage. The active ingredients of the composite microbial agent include 15 percent to 30 percent of bacillus stearothermophilus, 10 percent to 25 percent of bacillus licheniformis, 10 percent to 25 percent of zymomonas, 10 percent to 25 percent of agrobacterium, 15 percent to 30 percent of saccharomyces cerevisiae and 10 percent to 25 percent of candida, which are blended together. The composite microbial agent is prepared according to the following method: (1) the strains, which are preserved under the temperature of 4 DEG C, are respectively inoculated in ordinary broth media, the inoculation amount is 0.1 percent (v/v), and under the temperature between 28 DEG C and 37 DEG C, activating culture lasts for 8 to 18 hours; (2) the strains are then respectively transinoculated into ordinary broth media to be cultured for 8 to 18 hours under the temperature between 28 DEG C and 34 DEG C, the inoculation amount is between 0.1 percent (v/v) and 0.5 percent (v/v), and after culturing, microbial liquids are blended according to the proportion. The composite microbial agent can be used to preprocess kitchen garbage, inhibit the reproduction of pathogenic microorganisms in the kitchen garbage and reduce the deterioration and stink of the kitchen garbage, so that the kitchen garbage can be conveniently processed later, so the application prospect of the composite microbial agent is wide.

The invention relates to bidirectional burkholderia as well as a culture method and an application thereof and belongs to the field of plant protection (microbial pesticide). The preservation number of the bidirectional burkholderia is CCTCC NO: M2013145. The culture method of the bidirectional burkholderia comprises the following steps: adding 1ml of 2-day-aged seed bacterial strain to an LB (Lysogeny broth) culture medium with the liquid loading of 50ml under a fermentation condition, and culturing for 20-24 hours at the temperature of 25-30 DEG C at the rate of 200r/min. The bidirectional burkholderia has a remarkable inhibition effect on rice sheath blight disease, rice blast, colletotrichum musae, oxysporum, fusarium asiaticum, peronophythora litchi, sugarcane alternaria tenuis, cladosporium fulvum, xanthomonas oryzae, bacterial leaf steak and the like, can be used for the biological prevention and control of plant pathogenic bacteria, has the advantages of low toxicity, easy degradation, environmental friendliness and the like and has a bright application prospect.

The present invention is a method for preparing a antifungal formulation containing a high concentration of spores comprising providing Streptomyces bacteria with sporulation competence; propagating the bacterial strain for obtaining spores under an appropriate condition, collecting the spores, preparing the spores to obtain a small scale of the spore biomass as a seed inoculum, and culturing the seed inoculum at 28 to 37° C., 80 to 250 rpm, 0.25 to 0.75 vvm and pH at 5.0 to 8.0 to obtain a large scale fermentation of the bacteria in a suitable broth medium. The present invention further comprises a antifungal formulation containing Streptomyces spp. comprising a high concentration of spores and a liquid medium for culturing spore biomass of Streptomyces spp.

The present invention is directed to a medium, broth or agar, and a method of utilizing the same, in order to isolate and/or identify anaerobes from a mixed sample that contains facultative microorganisms. The medium contains an inhibitor of the electron transport system, such as a salt of azide (N₃⁻), cyanide (CN⁻) or related compounds. These inhibitors are present in an amount sufficient to limit the growth of facultative microorganisms under anaerobic conditions while not inhibiting the growth of the anaerobe microorganisms. Preferably, the inhibitor is present in the amount of from about 0.1 mg/ml to about 1.0 mg/ml in broth medium, and from about 0.01 mg/ml to 1.0 mg/ml in agar medium.

The invention relates to a detection medium and a detection method for Cronobacter in food. The detection method of the present invention utilizes the activity of alpha-glucosidase produced by Cronobacter to detect Cronobacter in liquid culture medium, and can identify Cronobacter while carrying out selective enrichment. The Cronobacter broth medium of the present invention may comprise 18-20 parts by weight of mixed peptone; 3-5 parts by weight of lactose; 2-3 parts by weight of K2HPO4; 2-3 parts by weight of KH2PO4; 20-34 parts by weight of sodium chloride 0.08-0.15 parts by weight of sodium lauryl sulfate; 0.009-0.011 parts by weight of vancomycin; 0.07-0.10 parts by weight of 4-methylumbelliferone-α-D-glucopyranoside; pH6.8 ± 0.2, the best Culture temperature, 36°C. The invention also discloses a reagent kit for the above detection method. The invention has the advantages of shortening the detection time and avoiding subsequent tedious identification operation steps, thereby improving the detection efficiency of Cronobacter.

The invention relates to dialdehyde cellulose grafted epsilon-polylysine antibacterial material, prepared by oxidizing cellulose into dialdehyde cellulose, and using the dialdehyde cellulose as a supporter to modify with epsilon-polylysine under a certain aldehyde group molar ratio through grafting reaction so that epsilon-polylysine is grafted to dialdehyde microcrystalline cellulose. The processto prepare the dialdehyde cellulose grafted epsilon-polylysine antibacterial material is simple; the dialdehyde cellulose grafted epsilon-polylysine antibacterial material has no toxic and side effects and has good antibacterial activity; with penicillin acting as a positive control and LB (lysogeny broth) liquid medium as a negative control, the material herein has minimum inhibitory concentrations of 7.5 mg/mL, 15 mg/mL and 30 mg/mL respectively for E. coli and S. aureus, S. typhimurium, and S. lutea and B. subtilis. A new idea is provided for the research and development of novel antibacterial materials.

The invention discloses a method for accurately detecting toxicity of water quality by using a photobacterium toxicity test, and the method comprises the following steps: carrying out solid culture on photobacterium in an LB (lysogeny broth) solid culture medium, inoculating the obtained photobacterium strain into a liquid LB culture medium, and culturing; centrifuging the liquid culture medium containing photobacterium, and carrying out suspension by using aseptic normal saline to obtain a bacterium suspension with the OD (Optical Density) value of 0.4-0.9; and mixing a water sample to be detected with the bacterium suspension, and carrying out the photobacterium toxicity test to detect the photobacterium inhibition rate of the water sample to be detected. In the method disclosed by the invention, the photobacterium is processed, so that both organic toxic substances and inorganic toxic substances can inhibit the luminous intensity of the photobacterium, thereby enhancing the toxicity detection accuracy of water quality by using the photobacterium toxicity test, and solving the problem that the photobacterium can not properly reflect the comprehensive toxicity of the water quality. The method disclosed by the invention has the advantages of low time consumption, high sensitivity and accurate result, and is simple and convenient to operate.

The invention provides a fermentation method for high-content 12[alpha]-hydroxysteroid dehydrogenase fermentation liquor, and belongs to the technical field of biology and medicines. the fermentationmethod comprises the following steps of: inoculating a spare recombinant escherichia coli containing a 12[alpha]-hydroxysteroid dehydrogenase expression gene into a novel sterile fermentation medium as a strain according to an inoculation amount of 2-6% of volume fraction to be cultured; and after 2h, carrying out fed-batch by a fed-batch induced medium totally for 8h. By use of the fermentation method, in a process that the spare recombinant escherichia coli containing the 12[alpha]-hydroxysteroid dehydrogenase expression gene is taken as the strain to ferment and produce the 12[alpha]-hydroxysteroid dehydrogenase (12[alpha]-HSDH), a high-nutrition low-cost novel medium is used for replacing a traditional LB (lysogeny broth) medium, the fed-batch induced medium is adopted, the bacteria OD600 (optical density) of culture bacteria is 50-60, and meanwhile, the enzyme activity of the 12[alpha]-hydroxysteroid dehydrogenase is 700-900U/L.

The invention provides a method for producing antioxidant of microbial food, comprising: respectively culturing palustris, wine spore hansenula and lactobacillus bulgaricus on each culture medium; respectively vaccinating obtained product into a lysozyme broth, culturing in a shake flask; adding sterilized fermenting substrate into the fermenting pot, vaccinating the cultured products of culturing palustris, wine spore hansenula and lactobacillus bulgaricus into the fermenting substrate; sterilizing the liquid obtained; aerating and then obtaining the antioxidant of microbial food. After the invention enters into human body, the contained anti-free radical substance can resist excessive free radicals in body, so as to avoid oxidative damage to tissue and effectively remove the active oxygen free radical produced in human body; the normal mechanism of cell membrane is protected, and the normal function of active molecule in the body is ensured.

The invention provides a method for synthesizing PHA (Phytohemagglutinin) by using pseudomonas putida KT2442. The method comprises the following steps of: 1, mixing a small quantity of pseudomonas putida KT2442 in an LB (lysogeny broth) culture medium, and culturing to obtain a seed culture solution containing strains; and 2, adding the seed culture solution obtained in the step 1 and a carbon source to the culture medium, culturing, separating, quick-freezing, drying, and refining to obtain a final product PHA. According to the method, the pseudomonas putida KT2442 in culture wastewater is used, the supply of the carbon source is adjusted, and correlated matrix octane acid and non-correlated matrix glucose are respectively added in the culturing process, thus the final product PHA is obtained. The product obtained by using the method has strong decomposability, low glass transition temperature, good flexibility and adhesion and can be applied to the medicine field, and therefore, the production has a bright application prospect.

The invention belongs to the technical field of agricultural microbiology, and particularly relates to the separation screening and fermentation cultivation of phosphorus-dissolving pseudomonas putida L13. A phosphorus-dissolving pseudomonas putida L13 strain with the capacity of dissolving phosphorus is obtained through separation, and the preservation No. is CCTCC No.2010342. The fermentation process comprises the following steps of: (1) taking L13 bacterial strain slope seeds for streak cultivation on an LB (lysogeny broth) culture medium flat plate to obtain first-level seeds; (2) inoculating a first-level seed single bacterial colony into an LB culture medium shake flask to make OD (optical density) 600 be 2.0 to obtain second-level seeds; (3) inoculating the second-level seeds into a shake flask for fermentation cultivation to obtain the pseudomonas putida; or inoculating the second-level seeds into a fermentation tank so as to obtain the pseudomonas putida through optimized cultivation. The pseudomonas putida strain disclosed by the invention grows rapidly, the yield of the pseudomonas putida is high, and the viable count can reach more than 10 billions. The phosphorus-dissolving amount of the phosphorus-dissolving pseudomonas putida in a PKO (Pikovskaya') culture medium in which 1-3g/L of calcium phytate is the unique phosphorus source can reach 120-220mg/L, and the phosphorus-dissolving amount of the phosphorus-dissolving pseudomonas putida in a PKO culture medium in which 4-6g/L of tricalcium phosphate is the unique phosphorus source can reach 300-500mg/L.

The invention discloses determination of microbial inhibition potency of macleaya cordata extract. The determination comprises the following steps: 1) extracting a macleaya cordata raw material; 2) preparing a to-be-determined sample solution; 3) preparing a standard sample solution; 4) preparing test culture medium; 5) determining antibacterial potency; and 6) respectively taking 0.1ml bacteria solution of escherichia coli, salmonellas, staphylococcus aureus and bacillus subtilis in a logarithm growth period in a control group, uniformly coating the surface of a common broth medium, taking sterilized filter paper pieces with the diameter of 6mm, immersing in 1.5mg/ml, 3.0mg/ml and 4.5mg/ml standard sample solution for 38-42 minutes, then taking out, putting into an incubator at the temperature of 37 DEG C, culturing for 24 hours, then observing, and recording the average value of diameters of inhibition zones of three filter paper pieces for each kind of bacteria. The determination disclosed by the invention has the advantages that a theoretical foundation is provided for applying macleaya cordata to replace antibiotics in piglet feed through study on the determination of the macleaya cordata extract; and by adopting a contrast test, content of macleaya cordata effective ingredient in the macleaya cordata extract can be determined more accurately, and use of the macleaya cordata is accurately controlled.

The invention discloses a method for producing living paratyphoid vaccine for piglets and a product thereof. The method comprises the following steps: (1) preparing primary strains; (2) preparing secondary strains; and (3) culturing bacterial liquid: inoculating the secondary strains into a culture medium for culturing, after culturing, immediately adding a stabilizing agent which is sterilized and preheated to 37 DEG C to ensure that the vaccine contains 1.5 percent of gelatin and 5 percent of sucrose, and mixing uniformly to obtain the bacterial liquid, wherein the culture medium in the step (3) consists of a common broth medium and a synthetic medium in the volume ratio of 2:1. In the living paratyphoid vaccine for piglets produced by the method, the cultured bacteria count is over 50 billion per milliliter; the maximum cultured bacteria count is 58 billion per milliliter; and the freeze-drying death rate is reduced to 20-40 percent. The method for producing the living paratyphoid vaccine for the piglets has the advantages of high bacterial count of the living vaccine, low freeze-drying death rate, easy production, high yield, low production cost, and the like.

The invention discloses Lactobacillus plantarum and application thereof in producing phenyllactic acid. The Lactobacillus plantarum is classified and named as Lactobacillus plantarum, has a strain number of CXG9, is preserved in China General Microbiological Culture Collection Center on March 1, 2021, has a preservation address of Institute of Microbiology of Chinese Academy of Sciences, 3#, No. 1 Yard, West Beichen Road, Chaoyang District, Beijing, and has a preservation number of CGMCC No. 21849. The Lactobacillus plantarum CXG9 is separated from fermented pickled vegetable stems for the first time, and the Lactobacillus plantarum CXG9 is found to be capable of producing phenyllactic acid at high yield; and the Lactobacillus plantarum CXG9 is fermented in an MRS broth culture medium, and the content of phenyllactic acid in fermentation liquor reaches 132.94 micrograms/mL.

The invention belongs to the technical field of biological culture and particularly relates to method for transforming lignin into phenolic acid by using lentinus edodes fermentation culture. The method comprises the steps of taking lignin as an exogenous additive, and preparing an improved potato dextrose broth medium containing a certain concentration of lignin; inoculating a lentinus edodes mycelium, culturing the lentinus edodes mycelium for a period of time and then collecting the lentinus edodes mycelium; and extracting and obtaining phenolic acid substances from the collected lentinus edodes mycelium. According to the method provided by the invention, growth of the lentinus edodes mycelium and biotransformation of phenolic compounds can be promoted through adding an appropriate concentration of lignin.

The invention relates to a staphylococcus simulans BP10. The staphylococcus simulans BP10 is preserved in China General Microbiological Culture Collection Center with a preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 16592. The invention also relates to a staphylococcus simulans BP10 suspension and a preparation method thereof. The staphylococcus simulansBP10 is prepared by inoculating the staphylococcus simulans BP10 into a LB (lysogeny broth) culture medium for cultivation and then performing centrifugal collection, washing and sterile water resuspension precipitation. The invention also relates application of the staphylococcus simulans BP10 to sausage fermentation and discloses a sausage fermenting agent. The sausage fermenting agent is composed of a lactobacillus plantarum b-2 suspension and the staphylococcus simulans BP10 suspension as a volume ratio of 3:1. When applied to sausage fermentation, the sausage fermenting agent can improvethe quality of fermented sausage products, increase the flavor of the products, improves the structural texture of the products, shorten the fermentation cycle and improve safety of the products.

The invention provides a bacteria culture medium product containing a feather meal as a plant leftover. The bacteria culture medium product is developed by replacing peptone in an LB (Lysogeny Broth) culture medium by the feather meal as the plant leftover and can be used for effectively culturing model escherichia coli, bacillus subtilis, other gram-negative bacteria and other gram-positive bacteria by virtue of liquid shake flask fermentation, wherein the feather meal used by the product is prepared by the steps of carrying out acidolysis on feathers, extracting plant leftovers of a part of amino acid, and carrying out neutralization and concentration to obtain the feather meal. Whether partly or wholly replacing the peptone in the LB culture medium, the bacteria culture medium product can both be used for effectively carrying out liquid fermentation on a strain for test. Meanwhile, By virtue of adopting a novel feather meal culture medium, the biomasses of the model escherichia coli and the bacillus subtilis in a stable period can be remarkably increased.

The present invention is a method for preparing a biocontrol formulation by the following steps. AStreptomyces bacterium is selected simultaneously with superior sporulation ability, chitinase activity and antagonistic effectiveness against plant fungal pathogens by culturing with fungal pathogens, wherein the selected Streptomyces bacterium is cultured on a chitin-limited plate to assess the ability of sporulation and chitinase activity, and the Streptomyces bacterium with superior sporulation ability is the strain with producing powder-like and maturity form spore chains. Spores from the selected bacterium is collected. The obtained spores are taken as a seed inoculum. Then the seed inoculum is cultured to directly yield a broth medium containing at least 10⁹ viable spores/ml which the spores contained in the broth medium are well suspended. The biocontrol formulation is obtained. The present invention further provided a biocontrol formulation containing a high concentration of Streptomyces spp. spores.

The invention provides a probiotic composition for inhibiting streptococcus mutans and application of the probiotic composition, and relates to the technical field of probiotics. The probiotic composition comprises a bacterial solution formed by co-culturing lactobacillus rhamnosus and lactobacillus fermentum, a bacterial solution formed by co-culturing bifidobacterium animalissubsp.lactis and lactobacillus casei and/or a bacterial solution formed by co-culturing lactobacillus paracasei and bifidobacterium infants; and an inoculation culture medium for co-culturing comprises an MRS broth culture medium, and co-culturing is anaerobic culturing at 37 DEG C for 24 h. The probiotic composition shows a higher antibacterial effect on inhibiting dental caries generation bacteria (streptococcus mutans) than a single strain, and can be widely applied to probiotic health food through safety evaluation.

The invention provides a selective enterococcus enrichment medium, the technical scheme of which is composed of the following components: protein powder, peptone 5.0g, yeast extract powder 2.5g, beef extract 3.0g, glucose 20.0g, lactose 4.0g, chlorine Sodium chloride 5.0g, sodium azide 2.5g, ox bile salt 10.0g, 0.1% bromocresol green 20% ethanol aqueous solution 6.0ml, distilled water 1000ml. Weigh 5.0g of peptone, 5.0g of peptone, 2.5g of yeast extract powder, 3.0g of beef extract, 20.0g of glucose, 4.0g of lactose, 5.0g of sodium chloride, 2.5g of sodium azide, and 10.0g of ox bile salt in a 1000ml Erlenmeyer flask In the above-mentioned Erlenmeyer flask, measure 1000ml of distilled water, heat to dissolve, adjust the pH value to 6.0, then add 6.0ml of 0.1% bromocresol green 20% ethanol aqueous solution, pack 50.0ml in a 150ml Erlenmeyer flask and wrap it in the temperature Sterilize for 15 minutes at 121°C. Beneficial effects of the present invention: compared with AC broth culture medium, the color change is more obvious, and the result is easier to observe.

The invention relates to the cross field of a molecular biotechnology and a microbiological technique, in particular to a method specifically separating sphingomonas yanoikuyae by combining a streptomycin resistance surface plate based on special primer polymerase chain reaction (PRC). The method comprises the following step: coating a suspension solution of a sample to be detected on a lysogeny broth (LB) culture medium fixed flat plate; selecting yellow bacterial colony; repeatedly scribing to separate purity; extracting pure bacterial strain gene group DNA after being cultured by liquid; amplifying a 16SrDNA segment by adopting the sphingomonas special primer PCR; and carrying out positive amplification and amplification to obtain a segment of 504bp PCR product which is sphingomonas. With adoption of the method, the culturable sphingomonas can be directly and specifically separated from the soil; special species are counted; and a bacterial strain resource of the culturable sphingomonas can be obtained.

The invention belongs to the technical field of bioengineering, and discloses a gene deletion type klebsiella pneumoniae attenuated live vaccine, a preparation method and application. The preparation method comprises the following steps: preparing a KP1-RS12260 gene deletion strain of a klebsiella pneumoniae virulent strain NTUH-K2044; culturing the KP1-RS12260 gene deletion strain of the klebsiella pneumoniae virulent strain NTUH-K2044 for multiplication with a lysogeny broth (LB) medium overnight on a bacteria shaking table; and diluting multiplicative strains into a fresh LB culture solution according to a ratio of 1:100 the next day for culture until OD600 is equal to 1.4, performing centrifuging for 10min and collecting centrifugal precipitates, then washing bacteria twice with normal saline, and performing re-suspending with the normal saline until the concentration of the bacteria reaches 10<5>CFU/ml. The invention can effectively solve the problems of limited protection range, low protection effect and the like of klebsiella pneumoniae vaccine components and subunit vaccines at present.

The present invention relates to new contamination resistant artificial media for the routine cultivation of P. salmonis. In particular, it discloses first a liquid media based named Austral-SRS Broth that extensively require iron and sodium chloride; and, second, a blood-free agar media comprising tryptone soy with either ferric or ferrous salts (Austral-TSFe) agar or the use of hemoglobin (Austral-TSHem) as a source of iron in the agar. Also disclosed is a method for their preparation as well as their application in the development of antigens and low cost vaccines for protection against Piscirickettsiosis and the use of the medias for a kit that evaluate the antibiotic resistance appearance in different strain of P. salmonis isolated from the salmon industry.

A preparation method of an efficient active composite corruption decoloring inoculant comprises the following steps: carrying out corruption fermentation of a broth medium, dumping bacterial dyed wastewater, carrying out domestication culture, separating and screening three decolored bacteria, and finally determining Bacillus cereus, Clostridium perfringens and Shewanella putrefaciens according to the form characteristics and the culture characteristics of the three decolored bacteria and biochemical experiments of the three decolored bacteria; and respectively inoculating the three decolored bacteria preserved at a low temperature into an inclined plane nutritional agar culture medium, carrying out rejuvenation culture, carrying out composite culture of the three rejuvenated decolored bacterial in the broth culture medium to obtain a seed liquid, inoculating the seed liquid into a fermentation tank provided with the broth culture medium, and carrying out enlargement fermentation culture to prepare the efficient active composite corruption decoloring inoculant. The efficient active composite corruption decoloring inoculant has the advantages of simple preparation process, stable performances, use convenience, storage at normal temperature without inactivation, and realization of the bacterial dyed wastewater decoloring rate of 100%.

The invention relates to an optimum growth condition of a Klebsiella oxytoca strain for degrading petroleum pollutants and a tolerance research method. The method comprises the following steps: preparing a tryptone soya broth culture medium for use in growth situation test and tolerance condition test; performing splitting charging on the culture medium, sealing, sterilizing under high-pressure steam of 121 DEG C for 20 minutes, and cooling to the room temperature for later use; adding a strain into the tryptone soya broth culture medium for use in the growth situation test, testing the cell concentration of a culture sample through an OD600 test method, and drawing a strain growth curve; adding thalli into the tryptone soya broth culture medium for testing different tolerance conditions respectively, performing three parallel treatments for each group, culturing in a shaking table of which the speed is 220rpm, sampling after a stabilization period, testing the cell concentration through the OD600 test method, and drawing a strain tolerance curve. The Klebsiella oxytoca strain has a good oil removing effect in the temperature range of 20-40 DEG C, the acidity and alkalinity range of pH5-pH8 and the salinity range of 0.5-3 percent.