58results about How to "Fast extraction method" patented technology

The invention discloses a Lamb wave damage probability imaging method for damage monitoring of a composite plate structure, which belongs to the field of monitoring of engineering structure health of composite plates. According to the invention, the technology of excitation / sensing arrays is utilized; reference signals of the composite plate structure in a sound state and monitoring signals of the composite plate structure in a damage state are acquired by loading Lamb wave; then the means of wavelet analysis is employed to extract an energy difference coefficient of damage, and the position where damage is located is predicated; finally, a structure damage image is obtained by using the probability imaging method. The invention enables rapid and effective damage identification of the composite plate structure to be realized and high precision in damage identification to be obtained, and has a good engineering application value.

The invention discloses a method for preparing high-purity asiaticosid. The method mainly comprises the following steps of: preparing an asiaticosid extracting solution by using an ethanol aqueous solution under an ultrasonic condition, and concentrating the extracting solution to obtain an extract; and extracting an extract solution with petroleum ether and ethyl acetate respectively to obtain an aqueous solution, extracting with a mixed solvent of n-butyl alcohol and ethyl acetate, combining upper phase organic solutions, decolorizing with active carbon, concentrated under reduced pressure, performing vacuum drying to obtain asiaticosid, and recrystallizing the asiaticosid to obtain high-purity asiaticosid. The process operation method has the advantages of easiness, no need of column chromatography separation in an operating process, short production period, large treating capacity, high product purity and suitability for industrial mass production.

The invention provides a pig feed additive capable of effectively improving pig immunity and promoting pig growth. The pig feed additive is composed of astragalus polysaccharide, acanthopanax powder, fermented traditional Chinese medicine powder, muskmelon pedicel powder, tangerine peel powder and the rest carrier, the carrier is a mixture of wheat bran and defatted rice bran at a mass ratio of 1:1. The pig feed additive can remarkably lower incidence of pigs, improve immunity of the pigs and increase growth rate of the pigs, can replace or partly replace application of chemosynthesis additives in feed and has wide market prospect.

The invention discloses a method for preparing high purity chlorogenic acid. The method comprises the following steps of: preparing a lonicera confusa extract from lonicera confuse serving as a raw material by using a mixed solvent of ethanol and water under the ultrasonic condition, dissolving the extract in water, selectively extracting chlorogenic acid from the aqueous solution, performing back-extraction on extract liquor by using an aqueous solution of polyacrylamide to remove partial high-polarity impurities, decoloring the extract liquor subjected to back-extraction by using active carbon, and recrystallizing to obtain chlorogenic acid crystals. The method is easy to impellent, the production period is short, the yield and purity of the product are high, and the method is suitable for industrial production.

The invention discloses a virus nucleic acid extraction kit. The virus nucleic acid extraction kit comprises a lysis solution, a first rinsing solution, a second rinsing solution, an elution solution,protease K and magnetic beads; the lysis solution comprises sodium perchlorate with the concentration of 1.0-5.0 mol / L, Tris-HCl with the concentration of 10-200 mmol / L, EDTA with the concentration of 1-50 mmol / L, and Triton X-100 with the concentration of 50-100 microlitre / milliliter; the first rinsing solution is sodium perchlorate solution with the concentration of 2.0-2.5 mol / L, and the solvent of the sodium perchlorate solution is 70-80% ethyl alcohol; the second rinsing solution comprises DEPC water and absolute ethyl alcohol, wherein the volume ratio of the DEPC water to the absolute ethyl alcohol is (3-4):(6-7); the elution solution is Tris-HCl-DEPC water solution with the concentration of 8-12 mmol / L. The invention further discloses a method for extracting viral nucleic acid by using the extraction kit. Through optimization of the reagent formula, the extraction kit has the advantages of being rapid and efficient and avoiding pollution, a large amount of time and reagent canbe saved, and the extraction efficiency of the virus nucleic acid is improved.

The invention discloses a pig feed additive for improving intestinal health and boosting immunity and belongs to the field of feed additives. The additive is prepared from compound plant polysaccharide, complex enzyme preparation, lactobacillus plantarum powder, lactobacillus buchneri powder, stevia rebaudiana powder, fructus chebulae powder, orange peel powder, arginine, glutamine dipeptide, alpha-oxoglutarate, cystine, taurine and a carrier, wherein corncob powder and peanut shell powder serve as the carrier. By the adoption of the additive, pig intestinal health can be improved, intestine development of live pigs can be promoted, the feed digestibility and absorptivity of live pigs can be improved, pig growth can be promoted, marketing period can be shortened, economical benefits can be increased, and feeding effect can be improved. Meanwhile, the immunity of pigs can be improved, the morbidity of pigs can be reduced, and application prospects are quite broad.

A method for extracting flat-band voltage and threshold voltage of an MOSFET (metal-oxide-semiconductor field effect transistor) based on current generation of a grid-control drain electrode belongs to the filed of micro electronic technique, is used for extracting VFB and VTH through the variation of grid-control generated current caused by the fact that a channel of the MOSFET changes from an accumulation region, an exhaustion region to a transoid region, and includes the following steps: an electrode at the source end of the MOSFET is hung in the air, a small drain voltage VD is exerted on the drain electrode, and the absolute valve of the VD is smaller than or equals to 0.2 V; the gate voltage VG is scanned, the channel changes from the accumulation region to the transoid region, and the grid-control generated current is measured under the drain bias VD; secondary partial differentiation operation for the obtained grid-control generated current curve IGD is performed to obtain the relation curve of the secondary derivative and the VG; the curve forms three peak value points, so as to obtain the peak value point corresponding to the rise edge and the peak value corresponding to the decline edge; the flat-band voltage VFB can be obtained through forming a vertical at the peak value point corresponding to the rise edge and enabling the vertical to be intersected with a grid voltage shaft; and the threshold voltage VTH can be obtained through forming a vertical at the peak value point corresponding to the decline edge and enabling the vertical to be intersected with the grid voltage shaft.

The invention relates to a method for extracting the whole blood DNA of bird species, which comprises the following steps: taking 22 mu l of blood to add into a centrifugal tube after the frozen blood is melted at the room temperature; and then, adding 860 mul of buffer solution, 100 mul of schizolysis solution and 15 mu l of prolease K with the total volume not exceeding 1000 mul. After the DNA is obtained by the extraction method of the invention, DNA sediments are dried by being placed in a vacuum drier or a baking oven with the temperature of 45 DEG C, the DNA is fully dissolved through adding 100 to 200 mu l TE solution according to the size of the sediments after a block mass becomes transparent, and the obtained materials are stored in a refrigerator at the temperature of 20 DEG C below zero. The extraction method does not relate to the use of virulent drugs of phenol and the like in the whole extraction process, and has no injury on bodies of operators. Compared with the existing extraction method, the invention has the advantages that the extraction method is safer, the minimum time consumption of the completion of the whole extraction process is within four hours, the extraction time is short, and the extraction is faster than that of the existing extraction method. The DNA extraction can be completed only through a micro amount of anticoagulation by using the extraction method of the invention, and the method is more efficient than the existing method.

The invention relates to a preparation method for high-purity jasminoidin, particularly a preparation method for high-purity jasminoidin based on liquid-liquid partitioning extraction, which comprises the following steps: pulverizing gardenia, soaking in water, carrying out ultrasonic-assisted extraction, filtering, combining the extraction liquids, and concentrating while depressurizing to obtain a gardenia extraction concentrate; regulating the pH value of the gardenia extraction concentrate to alkaline, and extracting while stirring at high speed by using a low-carbon alcohol as a solvent, thus obtaining an extraction liquid rich in jasminoidin; decolorizing the obtained extraction liquid, and concentrating the decolorized extraction liquid while depressurizing to obtain a decolorized concentrate; pouring the decolorized concentrate into an organic acid ester while stirring so as to crystallize and precipitate jasminoidin; and separating out crystallized jasminoidin precipitate, washing with the organic acid ester, and repeatedly washing the precipitate to obtain the high-purity jasminoidin. The preparation method provided by the invention completely discards chromatography, has the advantages of simple operation process, short production period, high efficiency and high yield and purity of the product, and is suitable for industrial production.

The invention discloses a method of extracting lagedium sibiricum type bitter principle from chicories. The method comprises the following steps: crushing chicory roots, performing extracting with water; performing filtering, and performing reduced pressure concentration to obtain a total extract; dispersing the total extract into water; performing extraction with ethyl acetate; performing reduced pressure distillation to obtain an ethyl acetate extract; and dissolving the ethyl acetate extract obtained in the second step and performing gradient elution separation, wherein a mobile phase A is ethyl acetate, a mobile phase B is formic acid, a mobile phase C is n-butyl alcohol and the elution condition comprises 0-4 min, 45% A, 50% B and 5% C; 4-10 min, 45%-40% A, 50%-55% B and 5% C; 10-14 min, 40%-30% A, 55% B and 5%-15% C; and 14-20 min, 30% A, 55% B and 15% C. The extracting method provided by the invention is convenient and feasible, and the purities of separated lactucin and lactucopicrin are both higher than 98.0%.

The present invention relates to biological technology product, and is one kind of human STR genotype testing kit. The testing kit consists of mainly DNA extracting liquid, HS-Taq, sample buffering solution, reaction mixture, primer mixture, Ladder, reference template, etc. The present invention is especially suitable for the test of Chinese people and has test result of PM less than 10E(-18) and RCP greater than 99.9999%. The present invention may be used widely in medical jurisprudence, archaeology, genetics, oncology and other fields.

The invention discloses a method for separating and purifying pear pollen tube vacuoles. The method mainly comprises the steps of pollen collection, pollen culture, protoplast release, protoplast purification, vacuole separation, vacuole purification and the like. A neutral red two-staining method is adopted, and pollen grains, vacuoles, protoplasts and water vacuoles can be clearly distinguished under an optical microscope. The method provided by the invention is high in speed and simple, not only can be applied to patch clamp researches, but also can be further applied to vacuole proteome researches, and has the advantages that vacuole membranes have smooth surfaces, and are low in pollution degree, and fewer materials and medicaments are required.

The invention relates to the technical field of exosome separation, and provides a kit for extracting adipose-tissue-derived exosome. The kit comprises protein A magnetic beads, a magnetic bead washing solution, an FABP4 antibody, a No.1 washing solution and a No.2 washing solution, wherein the FABP4 antibody can be combined with the adipose-tissue-derived exosome in to-be-detected serum, the protein A magnetic beads and the exosome-FABP4 antibody can have interaction, so that a protein A magnetic bead-FABP4 antibody-exosome compound is formed, the No.1 washing solution can remove the non-fatexosome ingredients in the serum, the No.2 washing solution can realize the dissociation of the interaction among the exosome, the FABP4 antibody and the protein A magnetic beads in the protein A magnetic bead-FABP4 antibody-exosome compound, and thus the adipose-tissue-derived exosome is specifically separated from the serum.

The invention discloses an extraction and purification method of dioscin. The extraction and purification method comprises the following steps of: after carrying out multiple circulating extraction treatment on a mixture of dioscoreaceae rhizome powder and organic solvents, filtering to obtain dioscin extracting solution, wherein each extraction treatment comprises microwave treatment and refluxing-method treatment which are carried out in sequence; purifying the dioscin extracting solution to obtain dioscin. The extraction and purification method disclosed by the invention has the beneficialeffects that the technical operation is simple, the use amount of the organic solvent is greatly reduced, the process time is shortened by 50% or more, acid and alkaline are not used, and the main organic solvents can be recycled respectively, so that the generation amount of 'three wastes' (waste water, waste gas and waste solid) after industrial production is greatly reduced.

The invention discloses a DNA extraction reagent, a kit and an extraction method. The invention firstly provides the DNA extraction reagent which comprises a lysis solution, a rinsing solution I, a rinsing solution II and an eluent, wherein the lysis solution comprises guanidine isothiocyanate, Tris-HCl, ethylene diamine tetraacetic acid (EDTA), NaCl, sodium dodecyl sulfate (SDS) and a protease inhibitor. The invention further provides the kit containing the DNA extraction reagent and the method for extracting DNA by using the DNA extraction reagent or the kit. The DNA extraction method is rapid, simple and convenient, is favorable for crushing fresh tissues or frozen tissues, is particularly suitable for rapid operation in surgery, has low relative extraction cost, has high quality of extracted nucleic acid, and can meet the detection requirements of qPCR, NGS, SNP typing and the like.

The invention relates to an application of a Polygonum multiflorum extract in controlling grey mould fruit rot of strawberry. The extract is Polygonum multiflorum earthnut which is extracted by sterile water. According to the invention, Polygonum multiflorum is taken as a plant source extract resource to extract antibacterial substances, and the extract has the inhibiting effect and a certain in-vitro control efficiency to mould fruit rot of strawberry and can be further developed to novel plant source bactericide.

The invention relates to an application of a radix paeoniae alba extract for controlling grey mould fruit rot of strawberry. The extract has a remarkable inhibiting effect and a certain in-vitro control efficiency to grey mould fruit rot of strawberry by way of inhibiting molecular spore germination, sclerotium germination and the like, and can be further developed to a novel plant source bactericide.

The invention discloses a simple and rapid plant DNA extraction method and belongs to the field of molecular biology. The method includes cutting off a small amount of leaves, rapidly grinding the leaves until uniform pulp is formed, adding an extracting liquid (including 20-50 mM of Tris (that is trihydroxymethyl aminomethane) and 13-25 mM of EDTA (that is ethylenediaminetetraacetic acid), with the pH of the liquid being 8.0), heating the extracting liquid at 70-90 DEG C for 10-15 min, and performing high-speed centrifugation for 1 min to obtain a DNA extract that can be directly used for PCR. Compared with present DNA extraction methods, the method has advantages of capability of being simple and rapid, a low cost, high stability, safety, no pollution, capability of achieving high-throughput operation, wide applicability, and the like. The method has extremely high applicability and practicality in processes of large-scale genetic mapping, molecular marker assisted breeding, crop variety identification, and the like.

The invention provides a DNA extraction method. The method comprises the following steps: suspending a sample in a TE buffer solution; performing cell lysis and DNA release; and aminating magnetic nano-particles to adsorb the DNA. According to the DNA extraction method disclosed by the invention, the detection sample is simply treated, the DNA in the sample is rapidly separted by virtue of the aminated magnetic nano-particles, and a magnetic complex is directly added into a PCR (Polymerase Chain Reaction) so as to realize rapid and sensitive detection. Moreover, the DNA extraction method is applicable to multiple detection samples.

The invention provides an extraction method and application of 2-(2-phenethyl) chromone components in agalloch eaglewood and application of the 2-(2-phenethyl) chromone components. The extraction method comprises the following steps: drying the agalloch eaglewood, crushing, performing ethanol extraction by using a flash method, and performing flash evaporation concentration to obtain a concentrated solution; carrying out molecular distillation on the concentrated solution to obtain a heavy component; and separating the heavy component by dextran gel chromatography to obtain a 2-(2-phenylethyl) chromone component; The 2-(2-phenethyl) chromone components mainly comprise 2-(2-phenethyl) chromone and 2-[2-(4-methoxy) phenethyl] chromone. The extraction method provided by the invention is simple, convenient and rapid, and the extracted 2-(2-phenethyl) chromone and 2-[2-(4-methoxy) phenethyl] chromone are high in purity, can effectively inhibit the activity of tyrosinase, and have a good application prospect in the aspects of whitening and removing freckles.

The invention relates to a preparation method of a fish bait for resisting marine pathogenic vibrios. The preparation method comprises the following steps: heating and steaming glutinous rice, adding ethanol with a mass concentration of 30%-70% while the glutinous rice is hot, and controlling a mass volume ratio of the glutinous rice to the ethanol to be (1:0.01)-(1:0.05) g / mL; uniformly mixing and cooling, sequentially adding rape seed cake powder and a fructus corni extracting solution in proportion, and uniformly stirring, chopping and drying to obtain the fish bait; wherein a mass ratio of the glutinous rice to the rape seed cake powder is 1:1 to 1:10, and a mass volume ratio of the glutinous rice to the fructus corni extracting solution is 1:0.1 to 1:10 g / mL. The fructus corni is taken as a raw material, a technology for extracting a fructus corni phenolic acid compound is optimized by adopting a Box-Behnken response surface method, the yield of the fructus corni phenolic acid compound is improved, and an activity of the extract in resisting marine pathogenic bacteria is improved. The prepared fish bait is environmentally friendly, safe, reliable and capable of effectively resisting marine pathogenic bacteria, and has high industrialization value.

The invention relates to a method for preparing a carbon source extracting solution capable of increasing the denitrification efficiency of a constructed wetland. The method comprises the steps of recovering, cleaning, dehumidifying and cutting a wetland plant material serving as a carbon source extracting substance, enabling the cut carbon source extract and a sulfuric acid solution which are in the mass ratio of 1: (810-880) to be subjected to hydrolysis reaction for 10-60 min at the temperature of 90-100 DEG C, and cooling, thereby obtaining the carbon source extracting solution. According to the method, the method is simple, and a plant carbon source is convenient in material obtaining, adequate in sources and low in cost and can be used for increasing the denitrification efficiency of the constructed wetland in low-carbon-nitrogen-ratio sewage treatment in a manner of serving as an external carbon source, so that the application effect is remarkable.

The application provides a method and system for extracting hydrothorax exosome. The system comprises a centrifugal separator, filtration equipment and a centrifugal separation column, wherein the filtration equipment can intercept substances of which the diameter is 0.45 [mu] m; the centrifugal separator is used for performing centrifugal separation on hydrothorax to obtain supernatant; the filtration equipment is used for filtering the supernatant to obtain filtrate; and the exosome is extracted from the filtrate through a centrifugal column method. According to the system disclosed by the invention, by a method of performing centrifuging once and performing filtering once, a conventional membrane compatible centrifuging column reagent kit is combined, so that high-quality exosome can beeffectively extracted from hydrothorax as low as 5mL, the extracted exosome is complete in structure and high in purity, and can be directly used for downstream detection experimental researches including physical and chemical detection, exosome nucleic acid extraction and the like, and therefore, the method for quickly, easily and conveniently extracting the hydrothorax exosome is provided.

The invention belongs to the technical field of biopesticides, and particularly provides a method for extracting a plant active substance capable of inhibiting bacterial wilt of tobacco. According to the method, amaranth leaves, taken as the raw material, are smashed after being dried, the amaranth leaf powder is extracted by analytically pure ethyl acetate, the collected extracting solution is put into a rotary concentration evaporator to be concentrated to form paste, and then the antibacterial substance is obtained. The method is simple, fast, convenient to operate, and easy for promotion and application.

The invention provides a kit for extracting silt microbe genome DNA (deoxyribonucleic acid) on the basis of a magnetic bead process. The method comprises the following steps: crushing silt microbe cells, removing impure proteins, recovering the microbe genome by a magnetic bead process to obtain the silt microbe genome DNA, and carrying out electrophoresis detection in 1% sepharose gel. The kit is mainly developed on the principle that the silyl magnetic beads specifically adsorb the genome DNA, is simple to operate, and greatly shortens the conventional silt microbe genome DNA extraction process, is more convenient and faster than the conventional silt microbe genome DNA extraction method, can implement large-scale extraction of the silt microbe genome DNA, and has certain economic value.

The invention relates to a method for extracting a sweet potato leaf extract. The method is characterized by comprising the following steps: weighing a certain amount of sweet potato leaves, naturally drying in the air, and grinding; extracting the ground sweet potato leaves by adopting a supercritical carbon dioxide fluid technology so as to obtain a greasy extract; and distilling by using a rotary evaporator to obtain dry extract, and grinding the dry extract by a conventional method to obtain the sweet potato leaf extract.

The invention discloses a concrete member benchmark-free damage probability imaging positioning method which specifically comprises the following steps: S1, manufacturing a detected concrete member and intelligent aggregate sensors made of the same material, arranging an intelligent aggregate sensor array in the concrete member, and ensuring to cover a to-be-detected area of the structure; s2, each path intelligent aggregate in the array intelligent aggregates sequentially serves as a driver, ultrasonic longitudinal wave signals meeting the excitation requirement are loaded through a signal generator and a power amplifier, other intelligent aggregates serve as sensors to collect ultrasonic longitudinal wave focusing signals which are directly received and subjected to time inversion processing, and structural response is reflected; and S3, response signals directly received by similar sensing paths. The invention relates to the technical field of structural health monitoring. According to the non-benchmark damage probability imaging positioning method for the concrete member, the number of used sensors is reduced, and rapid, stable, effective, safe and accurate recognition and positioning of member damage are achieved.

The invention discloses a method for separating and extracting lycopene from fermentation liquor. The method comprises the following steps: taking fermentation liquor containing lycopene, and collecting thalli; adding ethanol into the thalli to soak for 1-3h, and collecting precipitates; washing the precipitate with ethanol twice or more, and collecting bacterial powder; extracting the bacterial powder for 2-4 times by using ethyl acetate at 50-60 DEG C, and combining ethyl acetate phases to obtain an organic phase; concentrating the organic phase; adding ethanol, and crystallizing at 0-4 DEGC to obtain the lycopene crystal. By adopting the method provided by the invention to separate and extract lycopene from the fermentation liquor, the purity can reach 98% or above, and the yield can reach 75% or above. The method is simple in separation and purification process, high in product purity, low in cost and suitable for continuous production, and meets industrial requirements. The invention has important application value.

A method for extracting flat-band voltage and threshold voltage of an MOSFET (metal-oxide-semiconductor field effect transistor) based on current generation of a grid-control drain electrode belongs to the filed of micro electronic technique, is used for extracting VFB and VTH through the variation of grid-control generated current caused by the fact that a channel of the MOSFET changes from an accumulation region, an exhaustion region to a transoid region, and includes the following steps: an electrode at the source end of the MOSFET is hung in the air, a small drain voltage VD is exerted on the drain electrode, and the absolute valve of the VD is smaller than or equals to 0.2 V; the gate voltage VG is scanned, the channel changes from the accumulation region to the transoid region, and the grid-control generated current is measured under the drain bias VD; secondary partial differentiation operation for the obtained grid-control generated current curve IGD is performed to obtain the relation curve of the secondary derivative and the VG; the curve forms three peak value points, so as to obtain the peak value point corresponding to the rise edge and the peak value corresponding to the decline edge; the flat-band voltage VFB can be obtained through forming a vertical at the peak value point corresponding to the rise edge and enabling the vertical to be intersected with a grid voltage shaft; and the threshold voltage VTH can be obtained through forming a vertical at the peak value point corresponding to the decline edge and enabling the vertical to be intersected with the grid voltage shaft.

The invention discloses a polyacrylamide microsphere for rapid nucleic acid extraction and its preparation method and application. The polyacrylamide microsphere is mainly composed of acrylamide, methylenebisacrylamide, N-(3-aminopropyl) Composed of methacrylamide, ammonium persulfate and polylysine, and further cross-linked by glutaraldehyde. The present invention prepares polyacrylamide microspheres of various sizes through spot polymerization and improved inverse microemulsion polymerization. It not only has visible fluorescence and near-infrared fluorescence, but also can withstand various harsh conditions such as strong acid, strong alkali and high temperature. It is morphologically stable and has a high positive charge, which can be used to efficiently capture nucleic acid molecules such as DNA. The present invention also provides a new method for rapidly extracting DNA (3 to 5 minutes) from various biological samples using polyacrylamide microspheres. The polyacrylamide microspheres adsorbed with DNA can be directly added to the PCR reaction for DNA molecular routine and quantitative PCR amplification detection.