58results about How to "Fast extraction method" patented technology

The invention discloses a virus nucleic acid extraction kit. The virus nucleic acid extraction kit comprises a lysis solution, a first rinsing solution, a second rinsing solution, an elution solution,protease K and magnetic beads; the lysis solution comprises sodium perchlorate with the concentration of 1.0-5.0 mol/L, Tris-HCl with the concentration of 10-200 mmol/L, EDTA with the concentration of 1-50 mmol/L, and Triton X-100 with the concentration of 50-100 microlitre/milliliter; the first rinsing solution is sodium perchlorate solution with the concentration of 2.0-2.5 mol/L, and the solvent of the sodium perchlorate solution is 70-80% ethyl alcohol; the second rinsing solution comprises DEPC water and absolute ethyl alcohol, wherein the volume ratio of the DEPC water to the absolute ethyl alcohol is (3-4):(6-7); the elution solution is Tris-HCl-DEPC water solution with the concentration of 8-12 mmol/L. The invention further discloses a method for extracting viral nucleic acid by using the extraction kit. Through optimization of the reagent formula, the extraction kit has the advantages of being rapid and efficient and avoiding pollution, a large amount of time and reagent canbe saved, and the extraction efficiency of the virus nucleic acid is improved.

A method for extracting flat-band voltage and threshold voltage of an MOSFET (metal-oxide-semiconductor field effect transistor) based on current generation of a grid-control drain electrode belongs to the filed of micro electronic technique, is used for extracting VFB and VTH through the variation of grid-control generated current caused by the fact that a channel of the MOSFET changes from an accumulation region, an exhaustion region to a transoid region, and includes the following steps: an electrode at the source end of the MOSFET is hung in the air, a small drain voltage VD is exerted on the drain electrode, and the absolute valve of the VD is smaller than or equals to 0.2 V; the gate voltage VG is scanned, the channel changes from the accumulation region to the transoid region, and the grid-control generated current is measured under the drain bias VD; secondary partial differentiation operation for the obtained grid-control generated current curve IGD is performed to obtain the relation curve of the secondary derivative and the VG; the curve forms three peak value points, so as to obtain the peak value point corresponding to the rise edge and the peak value corresponding to the decline edge; the flat-band voltage VFB can be obtained through forming a vertical at the peak value point corresponding to the rise edge and enabling the vertical to be intersected with a grid voltage shaft; and the threshold voltage VTH can be obtained through forming a vertical at the peak value point corresponding to the decline edge and enabling the vertical to be intersected with the grid voltage shaft.

The invention relates to the technical field of exosome separation, and provides a kit for extracting adipose-tissue-derived exosome. The kit comprises protein A magnetic beads, a magnetic bead washing solution, an FABP4 antibody, a No.1 washing solution and a No.2 washing solution, wherein the FABP4 antibody can be combined with the adipose-tissue-derived exosome in to-be-detected serum, the protein A magnetic beads and the exosome-FABP4 antibody can have interaction, so that a protein A magnetic bead-FABP4 antibody-exosome compound is formed, the No.1 washing solution can remove the non-fatexosome ingredients in the serum, the No.2 washing solution can realize the dissociation of the interaction among the exosome, the FABP4 antibody and the protein A magnetic beads in the protein A magnetic bead-FABP4 antibody-exosome compound, and thus the adipose-tissue-derived exosome is specifically separated from the serum.

The invention discloses a DNA extraction reagent, a kit and an extraction method. The invention firstly provides the DNA extraction reagent which comprises a lysis solution, a rinsing solution I, a rinsing solution II and an eluent, wherein the lysis solution comprises guanidine isothiocyanate, Tris-HCl, ethylene diamine tetraacetic acid (EDTA), NaCl, sodium dodecyl sulfate (SDS) and a protease inhibitor. The invention further provides the kit containing the DNA extraction reagent and the method for extracting DNA by using the DNA extraction reagent or the kit. The DNA extraction method is rapid, simple and convenient, is favorable for crushing fresh tissues or frozen tissues, is particularly suitable for rapid operation in surgery, has low relative extraction cost, has high quality of extracted nucleic acid, and can meet the detection requirements of qPCR, NGS, SNP typing and the like.

The invention discloses a simple and rapid plant DNA extraction method and belongs to the field of molecular biology. The method includes cutting off a small amount of leaves, rapidly grinding the leaves until uniform pulp is formed, adding an extracting liquid (including 20-50 mM of Tris (that is trihydroxymethyl aminomethane) and 13-25 mM of EDTA (that is ethylenediaminetetraacetic acid), with the pH of the liquid being 8.0), heating the extracting liquid at 70-90 DEG C for 10-15 min, and performing high-speed centrifugation for 1 min to obtain a DNA extract that can be directly used for PCR. Compared with present DNA extraction methods, the method has advantages of capability of being simple and rapid, a low cost, high stability, safety, no pollution, capability of achieving high-throughput operation, wide applicability, and the like. The method has extremely high applicability and practicality in processes of large-scale genetic mapping, molecular marker assisted breeding, crop variety identification, and the like.

The invention provides an extraction method and application of 2-(2-phenethyl) chromone components in agalloch eaglewood and application of the 2-(2-phenethyl) chromone components. The extraction method comprises the following steps: drying the agalloch eaglewood, crushing, performing ethanol extraction by using a flash method, and performing flash evaporation concentration to obtain a concentrated solution; carrying out molecular distillation on the concentrated solution to obtain a heavy component; and separating the heavy component by dextran gel chromatography to obtain a 2-(2-phenylethyl) chromone component; The 2-(2-phenethyl) chromone components mainly comprise 2-(2-phenethyl) chromone and 2-[2-(4-methoxy) phenethyl] chromone. The extraction method provided by the invention is simple, convenient and rapid, and the extracted 2-(2-phenethyl) chromone and 2-[2-(4-methoxy) phenethyl] chromone are high in purity, can effectively inhibit the activity of tyrosinase, and have a good application prospect in the aspects of whitening and removing freckles.

The invention relates to a preparation method of a fish bait for resisting marine pathogenic vibrios. The preparation method comprises the following steps: heating and steaming glutinous rice, adding ethanol with a mass concentration of 30%-70% while the glutinous rice is hot, and controlling a mass volume ratio of the glutinous rice to the ethanol to be (1:0.01)-(1:0.05) g/mL; uniformly mixing and cooling, sequentially adding rape seed cake powder and a fructus corni extracting solution in proportion, and uniformly stirring, chopping and drying to obtain the fish bait; wherein a mass ratio of the glutinous rice to the rape seed cake powder is 1:1 to 1:10, and a mass volume ratio of the glutinous rice to the fructus corni extracting solution is 1:0.1 to 1:10 g/mL. The fructus corni is taken as a raw material, a technology for extracting a fructus corni phenolic acid compound is optimized by adopting a Box-Behnken response surface method, the yield of the fructus corni phenolic acid compound is improved, and an activity of the extract in resisting marine pathogenic bacteria is improved. The prepared fish bait is environmentally friendly, safe, reliable and capable of effectively resisting marine pathogenic bacteria, and has high industrialization value.

The invention relates to a method for preparing a carbon source extracting solution capable of increasing the denitrification efficiency of a constructed wetland. The method comprises the steps of recovering, cleaning, dehumidifying and cutting a wetland plant material serving as a carbon source extracting substance, enabling the cut carbon source extract and a sulfuric acid solution which are in the mass ratio of 1: (810-880) to be subjected to hydrolysis reaction for 10-60 min at the temperature of 90-100 DEG C, and cooling, thereby obtaining the carbon source extracting solution. According to the method, the method is simple, and a plant carbon source is convenient in material obtaining, adequate in sources and low in cost and can be used for increasing the denitrification efficiency of the constructed wetland in low-carbon-nitrogen-ratio sewage treatment in a manner of serving as an external carbon source, so that the application effect is remarkable.

The invention provides a kit for extracting silt microbe genome DNA (deoxyribonucleic acid) on the basis of a magnetic bead process. The method comprises the following steps: crushing silt microbe cells, removing impure proteins, recovering the microbe genome by a magnetic bead process to obtain the silt microbe genome DNA, and carrying out electrophoresis detection in 1% sepharose gel. The kit is mainly developed on the principle that the silyl magnetic beads specifically adsorb the genome DNA, is simple to operate, and greatly shortens the conventional silt microbe genome DNA extraction process, is more convenient and faster than the conventional silt microbe genome DNA extraction method, can implement large-scale extraction of the silt microbe genome DNA, and has certain economic value.

The invention relates to a method for extracting a sweet potato leaf extract. The method is characterized by comprising the following steps: weighing a certain amount of sweet potato leaves, naturally drying in the air, and grinding; extracting the ground sweet potato leaves by adopting a supercritical carbon dioxide fluid technology so as to obtain a greasy extract; and distilling by using a rotary evaporator to obtain dry extract, and grinding the dry extract by a conventional method to obtain the sweet potato leaf extract.