543results about How to "Inhibitory activity" patented technology

The invention provides application of an SRC inhibitor combined with a KRAS-G12C inhibitor to preparation of a medicine for treating KRAS-G12C mutant lung cancer and a medicine composition, and relates to the technical field of biological medicine, compared with independent use of the KRAS-G12C inhibitor, the scheme of the SRC inhibitor combined with the KRAS-G12C inhibitor can effectively inhibit proliferation and survival of KRAS-G12C mutant lung cancer cells, and the application of the SRC inhibitor combined with the KRAS-G12C inhibitor to preparation of the medicine for treating the KRAS-G12C mutant lung cancer can be used for preparing the medicine for treating the KRAS-G12C mutant lung cancer. The compound can effectively inhibit the activation of a KRAS-MARK pathway and an SRC-FAK pathway, the combination of the compound and the compound has a synergistic anti-tumor effect, and the compound and the compound can be directly combined for medication, can also be prepared into a combined preparation for treating KRAS mutant lung cancer, and have a good clinical transformation prospect.

The invention relates to a weight-losing enzyme and a preparation method thereof, and belongs to the technical field of biology, and the weight-losing enzyme comprises a fruit enzyme, a vegetable enzyme, a seed enzyme and a lotus leaf extract; the fruit enzyme is obtained by performing enzymolysis on mixed fruit pulp of apples, lemons, grapefruits and haws through pectinase, performing enzymolysis through pepsase and performing co-fermentation through bifidobacterium longum and lactobacillus acidophilus; the vegetable enzyme is obtained by performing enzymolysis on mixed vegetable pulp of wax gourd pulp, celery and spinach through pectinase, performing enzymolysis through pepsin and performing fermentation through lactobacillus plantarum and bifidobacterium adolescentis; the seed enzyme is obtained by carrying out trypsin enzymolysis on mixed powder of semen raphani and phaseolus calcaratus, then carrying out nattokinase enzymolysis and then fermenting with bifidobacterium breve; the lotus leaf extract is obtained by sequentially carrying out enzymolysis on lotus leaves through hemicellulase, snail protease and nattokinase. All the components are matched for use, so that the weight-losing food has the effects of promoting digestion, inhibiting fat absorption, regulating intestinal flora, promoting metabolism and the like, and has a good weight-losing effect.

The invention discloses a sea cucumber protein heptapeptide with functions of reducing blood pressure and blood sugar and application of the sea cucumber protein heptapeptide, and belongs to the technical field of bioactive peptide biology. The amino acid sequence of the sea cucumber protein heptapeptide is LPPGPFP. The sea cucumber protein heptapeptide can significantly inhibit the activity of ACE through a competitive inhibition mode, tolerates the gastrointestinal tract digestion environment, also has DPP-IV inhibition activity, can relieve hypertension and type 2 diabetes mellitus at the same time, and has an application value in preparation of products with the efficacy of reducing blood pressure and blood sugar due to multiple functions of the sea cucumber protein heptapeptide. The sea cucumber protein heptapeptide provided by the invention can significantly inhibit the activity of ACE through a competitive inhibition mode, has DPP-IV inhibition activity, has the characteristics of simple structure, safety, strong activity and the like, and has a wide application prospect.

The invention discloses application of an AXL inhibitor in preparation of a medicine for treating heart failure, and relates to the field of pharmacy. The invention provides application of an AXL inhibitor in a medicine for treating heart failure. The AXL inhibitor can remarkably inhibit abnormal activation of fibroblasts and can remarkably improve the heart function of a mouse with heart failure.

The invention provides anti-aging collagen peptide mask liquid and a preparation method thereof. The mask liquid is prepared from the following components in percentage by mass: 1 to 2 percent of tremella polysaccharide, 3 to 4 percent of herba portulacae extract, 2 to 3 percent of radix gentianae extract, 0.3 to 0.8 percent of nicotinamide, 1.2 to 1.6 percent of alpha-arbutin, 0.2 to 0.6 percent of chrysanthellum indicum extract, 0.7 to 1.5 percent of geranium flower / leaf / stem extract, 0.4 to 0.8 percent of aloe barbadensis extract, 0.7 to 1.3 percent of radix sophorae flavescentis extract, 0.3 to 0.9 percent of herba houttuyniae extract, 1.2 to 1.7 percent of herba centellae extract and the balance of water. The traditional Chinese medicine composition is prepared from the following raw materials in percentage by weight: 0.2 to 0.6 percent of fructus hippophae extract, 0.02 to 0.06 percent of peony root bark extract, 0.06 to 0.09 percent of scutellaria baicalensis root extract, 0.04 to 0.08 percent of rosemary extract, 0.01 to 0.04 percent of pearl powder and the like. The mask liquid prepared by the invention has an anti-aging effect.

The invention belongs to the field of comprehensive utilization of plant resources, and relates to a method for extracting amide components in Chinese prickly ash through a natural eutectic solvent assisted by superfine grinding. The method comprises the following steps: firstly, collecting a pepper raw material, drying the pepper raw material by a heat pump, and performing high-speed crushing and low-temperature superfine crushing to obtain pepper superfine powder; preparing a natural eutectic solvent from a hydrogen bond acceptor and a hydrogen bond donor, mixing the natural eutectic solvent with the pepper superfine powder, performing microwave-assisted extraction, centrifugally collecting supernate after extraction, and completing primary extraction; adding NDES into the remaining precipitate, and repeatedly extracting according to the primary extraction step; and repeatedly extracting for several times, combining the supernate, and finally concentrating and filtering with a filter membrane to obtain an extracting solution, thereby realizing the extraction of the amide components in the Chinese prickly ash. The method provided by the invention overcomes the defects of low extraction efficiency, environmental pollution caused by an extraction reagent, high equipment cost and the like in the existing technical means for extracting the amide components in the Chinese prickly ash, realizes efficient and green extraction of the amide components in the Chinese prickly ash, and has a wide application prospect.

The invention belongs to the technical field of biology, and particularly relates to a reagent combination and a kit for extracting nucleic acid through a paramagnetic particle method and application of the reagent combination and the kit. The reagent combination or the kit does not adopt beta-mercaptoethanol with pungent smell or dithiothreitol needing to be stored at low temperature as an antioxidant, but adopts tris (2-formylethyl) phosphine hydrochloride and / or metabisulfite as an antioxidant, belongs to common salt chemical reagent powder, can be stored at normal temperature, and can be used for preparing a reagent for the first time. Manual addition by experimenters before an experiment is not needed, so that nucleic acid (RNA) released by splitting decomposition can be effectively prevented from being oxidized and denatured, and the integrity of extracted nucleic acid (total RNA) fragments is ensured; especially, the metabisulfite is commonly used in food additives, does not cause harm to the body, and is non-toxic and non-volatile.

The invention relates to the technical field of daily chemicals, in particular to a white tomato whitening and repairing composition as well as a preparation method and application thereof. The composition comprises the following components in percentage by mass: 68-85% of jojoba oil, 10-25% of squalane and the balance of white tomato extract, wherein the total percentage is 100%. The white tomatoes are rich in colorless carotenoids (such as phytoene and tetrahydrolycopene), and have excellent antioxidant and whitening capabilities: melanin formation is inhibited, and the colorless carotenoids can inhibit tyrosinase activity, reduce melanin generation and fundamentally whiten the skin. Pigmentation caused by ultraviolet rays can be relieved through the anti-oxidation effect, and the color spots are prevented and faded. The skin color can be brightened after continuous use, so that the skin presents natural luster.

The invention relates to a tyrosinase antibody for inhibiting tyrosinase and application of the tyrosinase antibody, relates to the technical field of tyrosinase inhibition, and relates to an anti-tyrosinase antibody containing a heavy chain CDR and a light chain CDR with specific sequences or an antigen binding fragment of the antibody. The anti-tyrosinase antibody inhibits tyrosinase activity, and thus it is expected that the anti-tyrosinase antibody can be effectively applied to improvement of skin whitening or treatment of skin pigmentation diseases.

According to the method for promoting the fragrance release of the Phalaenopsis amabilis in the flowering stage, the influence of flowering days, different light intensities and different temperatures on the fragrance release in the full-bloom stage is compared, and the light and temperature adaptability of the fragrance of potted Phalaenopsis amabilis in the full-bloom stage is proved; and a basic basis can be provided for breeding aromatic phalaenopsis varieties and researching the action mechanism of the flower fragrance of important economic ornamental flowers. Meanwhile, the optimal culture medium formula of each link of tissue culture and rapid propagation of the phalaenopsis purpurascens is further deeply researched, discussed and screened, so that the phalaenopsis purpurascens can be developed and applied on an industrial scale.

The invention discloses a gene engineering exosome and a slow release method and application thereof, the gene engineering exosome is derived from human adipose-derived stem cells, si-tgfbr2 is loaded in the gene engineering exosome, the si-tgfbr2 is specific siRNA aiming at a Tgfbr2 gene, and the gene engineering exosome can regulate and control proliferation of VSMCs, inhibit transformation of the VSMCs from a contraction phenotype to a synthetic phenotype when the VSMCs are stimulated, and inhibit the growth of the VSMCs. According to the present invention, the gene engineering exosome can be used to maintain the normal structure and the function of the blood vessel, and the sustained release effect of the content in the gene engineering exosome can be achieved by wrapping the gene engineering exosome in the PHA biodegradable material, such that the local treatment concentration can be easily stabilized, the drug administration frequency can be easily reduced, the lasting time of the treatment effect can be easily prolonged, and the treatment gene can be effectively delivered to the target cell; the treatment effect is improved; when being used for modifying a small-caliber artificial blood vessel, the small-caliber artificial blood vessel can be improved in biocompatibility and functionality, and can be prevented from being implanted into the blood vessel for remodeling unbenign hyperplasia stenosis; the invention not only provides a new thought and method for the treatment of vascular diseases, but also has important clinical application value and popularization prospect.

The invention discloses a preparation method of a carbon dot nano-enzyme, which comprises the following steps: dissolving oxalic acid, polyethyleneimine and ferric nitrate in ultrapure water, heating, mixing and reacting, cooling to room temperature, centrifuging, filtering, dialyzing, and taking supernate to obtain the carbon dot nano-enzyme. The prepared oxalic acid carbon dot nano-enzyme is matched with acetylcholin esterase, acetylcholine, 3, 3 ', 5, 5'-tetramethyl benzidine and H2O2 to construct a colorimetric and fluorescent dual-mode detection method for glyphosate in fruits and vegetables. The oxalic acid carbon dot nano-enzyme is prepared by doping ferric nitrate and tetraethylenepentamine into oxalic acid serving as a precursor, the oxalic acid carbon dot nano-enzyme has excellent peroxidase-like activity, a colorimetric and fluorescent dual-mode detection method of glyphosate is constructed through the oxalic acid carbon dot nano-enzyme, dual-mode detection results can be mutually verified, and the detection result is accurate. And the accuracy and the reliability of a detection result are remarkably improved.

The invention discloses a gastrodia elata polypeptide extraction process, and belongs to the technical field of polypeptide extraction. According to the method, gastrodia elata cell membranes are broken through acetone pretreatment, so that protein and lipid are dissociated, trimethylsilyl ether passivates active groups, immobilized enzyme decomposes cellulose and adipose tissues through normal-temperature enzymolysis, a protein stabilizer prevents oxidation and inhibits enzyme activity, differential pressure extraction is combined with a sodium pyrophosphate buffer solution, polypeptide is promoted to be dissolved out, and activity is maintained; the high extraction rate of the gastrodia elata polypeptide and low-active group destructiveness are realized. Reaction sites and binding force are increased by epoxy groups on the surface of an immobilized enzyme carrier prepared by modifying corn starch, enzyme molecules are stable and appropriate due to good biocompatibility, enzyme molecule conformation is optimized through covalent binding, a rigid structure is enhanced, enzyme activity is kept at a high level for a long time, continuous and efficient enzymolysis is performed in gastrodia elata polypeptide extraction, and the gastrodia elata polypeptide extraction efficiency is improved. Compared with the traditional free enzyme, the gastrodia elata polypeptide enzyme has the advantages that the enzyme use cost is reduced, the enzyme consumption and waste generation are reduced, and an efficient and economical scheme is provided for gastrodia elata polypeptide extraction.

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus plantarum fermentation liquor and application thereof. The lactobacillus plantarum fermentation liquor provided by the invention is obtained by taking roses as a substrate and fermenting screened plant milk stalks under specific conditions. Experimental results show that the content of effective active ingredients such as total polyphenol, total polysaccharide and superoxide dismutase in rose fermentation liquor fermented by the lactobacillus plantarum is relatively high, and the fermentation effect of the rose fermentation liquor is far superior to that of commercially available common lactobacillus plantarum and other strains; besides, the rose fermentation liquor also shows obvious DPPH free radical and hydroxyl free radical scavenging ability, can effectively inhibit the activity of tyrosinase and improve the cell viability, has anti-aging and whitening effects, and can be widely applied to various foods and skin care products.

The present invention relates to the field of cosmetic technology, and in particular to a composition for regulating skin microecology containing a biological fermentation product and its application. The composition comprises inulin, micrococcal lysate, bifid yeast fermentation product lysate, rice fermentation product filtrate, lactobacillus / soybean milk fermentation product filtrate and water. The composition can effectively adjust the balance of skin microecology, and has excellent effects of removing wrinkles, preventing aging, and moisturizing.

The invention provides a preparation process of micromolecular cubilose peptide and a compound tablet thereof, and belongs to the technical field of protein and peptide. The preparation process of the micromolecular cubilose peptide comprises the following steps: freezing and crushing cubilose; and carrying out compound enzyme enzymolysis. The preparation method comprises the following steps: repeatedly freezing and crushing cubilose to prepare cubilose powder, adding the cubilose powder into deionized water, heating and stewing, and adding a compound enzyme formed by mixing neutral protease and flavourzyme for ultrasonic-assisted enzymolysis to obtain the micromolecular cubilose peptide, when the neutral protease and the flavourzyme are mixed according to the mass ratio of 1: (2.8-3.4) to conduct enzymolysis on the cubilose, hydrolysis can be conducted from multiple positions, smaller peptide fragments are formed, the hydrolysis degree is effectively improved, sialic acid originally combined with protein is also released along with hydrolysis of the protein, and therefore the content of sialic acid in the cubilose is increased. Therefore, the content of sialic acid in the prepared micromolecular cubilose peptide can be effectively improved.

The invention discloses a wine brewing rice pretreatment and fermentation method for reducing the drunkenness of white spirit. The rice pretreated by the method disclosed by the invention is subjected to liquor fermentation, and the fusel oil content is 1.2-1.4 g / L, which is reduced by 0.3-0.5 g / L compared with that of a control sample which is not pretreated by the soaking solution disclosed by the invention. The taste evaluation result of the wine body is as follows: the rice fragrance is comfortable and elegant, the taste is mellow, sweet and clean, the rich rice fragrance remains in the mouth, and the wine is plump and pleasant. After the wine liquid is aged, the comprehensive drunkenness Q ranges from 0.75 to 0.99 and is far lower than the comprehensive drunkenness Q of various commercially available baijiu (the comprehensive drunkenness Q is larger than or equal to 1) and a control sample (the comprehensive drunkenness Q is equal to 1.327).

The utility model discloses a movable radial artery puncture auxiliary device, and belongs to the technical field of medical equipment. Comprising a movable base which moves under the action of external force, a lifting component installed in the center of the top face of the movable base and an angle adjusting piece installed on the top of the lifting component in the longitudinal direction, the angle adjusting component reciprocates in the longitudinal direction under the action of the lifting component, and the angle adjusting piece has two degrees of freedom. The arm support is transversely placed and installed at the top of the angle adjusting piece, the corrugated support is arranged at the end of the arm support, and a restraint strap is installed at the end, away from the arm support, of the corrugated support. The angle adjusting piece is matched with the corrugated support and medical staff, adjustment is carried out according to the condition of a patient, the success rate of radial artery puncture catheterization and the comfort degree of the patient are increased, the restraint strap is matched, the upper limbs of the patient can be effectively prevented from moving or being not matched during radial artery puncture, operation is easy and convenient, and practicability is high.

The invention discloses a satiety sugar-controlling fat-reducing composition and a preparation method and application thereof, the satiety sugar-controlling fat-reducing composition comprises hydrogel particles, a white kidney bean extract, a mulberry leaf extract, alpha-lipoic acid, L-carnitine, a lotus leaf extract, medium chain triglyceride, polyethylene glycol and sorbitol, and the composition is a tablet. The components are matched with one another to effectively adjust the leptin level, improve appetite, increase energy consumption, adjust the fat metabolism process, reduce fat accumulation, improve fat consumption, inhibit the activity of alpha-amylase, reduce the conversion of carbohydrates into fat, block heat and fat intake, promote gastrointestinal motility and enhance the immunity of the human body. The effects of remarkably reducing the fat content and the body weight are achieved.

The invention discloses a long-acting anti-aging composition capable of quickly removing dark circles and application of the composition. The composition is prepared from the following components: beta-nicotinamide mononucleotide, glutathione, acetyl hexapeptide-8, acetyl tetrapeptide-5, snake venom-like peptide, arginine / lysine polypeptide, tocopheryl acetate and ergothioneine. According to the present invention, multiple functions are adopted, such that the effects of dark circle removing, repair, elasticity recovery, aging resistance and the like are provided so as to block the generation of dark circle, and the normal function of skin is ensured. The composition disclosed by the invention has the effect of quickly removing dark circles, and when the eye cream contains the composition, the eye cream can effectively remove the dark circles and has an obvious and quick effect within 15 minutes; after being used for a long time, the eye mask can remove dark circles and play a good control role.

The invention relates to the technical field of molecular biology, and particularly discloses application of miR-615 in ovarian granular cells. The miR-615 is applied to any one of the following items: preparing a product for promoting the activity and / or proliferation of ovarian granular cells; preparing a product for promoting generation of estradiol and / or progesterone; preparing a product for promoting the expression of the 3 beta-HSD and / or StAR gene; preparing a product for inhibiting ACSL4 gene expression; and preparing a product competitively combined with the lnc-GAM. According to the invention, porcine ovarian granular cells are used as a material, the expression level of lnc-GAM-miR-615 / ACSL4 regulatory axis is up-regulated or down-regulated in the porcine ovarian granular cells by using gene overexpression and interference technologies, and the expression of the lnc-GAM-miR-615 / ACSL4 regulatory axis is determined by detecting cell proliferation activity, hormone secretion level and apoptosis gene expression in combination with a dual-luciferase reporter gene test. And the regulation effect of lnc-GAM mediation on the apoptosis of the porcine ovarian granular cells is researched.

The invention relates to the technical field of food processing and functional drinks, and discloses a peptide-component-rich health-preserving liqueur which comprises the following components in percentage by weight: 50-65% of Maotai-flavor liquor, 10-20% of yellow wine, 3-7% of water, 10-20% of peptide components, 1-5% of plant extract and 5-10% of other components, the invention also discloses a preparation method of the health-preserving liqueur rich in peptide components, which comprises the following steps: S1, peptide treatment: carrying out directional enzymolysis on the peptide components, controlling the enzymolysis temperature at 40-50 DEG C and the enzymolysis time at 2-4 hours; and S2, microcapsule coating: coating the treated peptide components in cyclodextrin, gelatin or Arabic gum. By adopting a compound formula of multiple peptide components and combining technologies such as microcapsule coating, directional enzymolysis, low-temperature co-fermentation and nano-emulsification, the stability and bioavailability of the peptide components are remarkably improved, meanwhile, the flavor and taste of a wine body are optimized through a two-stage fermentation process, the wine body is more mellow and smoother, and the taste of the wine body is improved. The requirements of the market on the high-quality health-preserving liqueur are met.

The invention belongs to the field of tumor drugs, and discloses a radioactive hydrogel based on crosslinking of sodium alginate and metal ions and a preparation method and application thereof.The preparation method of the radioactive hydrogel comprises the following steps that lutetium chloride and metal ions are mixed, and a metal ion mixed solution is obtained; doping 177Lu radioactive isotope into the obtained metal ion mixed solution to obtain a metal ion cross-linking agent; and injecting a metal ion cross-linking agent into the sodium alginate solution under a vortex condition, centrifuging, and discarding the supernatant to obtain the radioactive hydrogel. The radioactive hydrogel (177Lu-RH) provided by the invention has good in-tumor colonization capability, can be directly injected into a tumor to realize local radiotherapy, has good radioactive stability, and can be stably retained at a tumor part for more than 120 hours, so that the stability of radioactive labeling in a treatment process is ensured.

The invention discloses carbon monoxide gas oxidative dehydrogenation equipment, which belongs to the field of carbon monoxide treatment and comprises a hollow shell, a gas inlet cover fixedly connected to the upper end of the hollow shell, a bottom plate fixedly connected to the inner wall of the hollow shell and a first exhaust port formed in the lower end of the bottom plate. A first partition plate and a second partition plate located below the first partition plate are further fixedly connected to the inner wall of the hollow shell, and a flow passing cavity is formed between the upper portion of the first partition plate and the air inlet cover. The cooling air ring can exhaust oxygen to the inner wall of the reaction through hole, it is ensured that the oxygen is evenly distributed on the inner wall of the reaction through hole, the oxygen is prevented from being concentrated in a certain area, and therefore the catalytic reaction efficiency is improved; it is ensured that the catalytic reaction is evenly conducted in the whole catalyst, the situation that the reaction efficiency is reduced due to insufficient oxygen or unsmooth airflow in a local area is avoided, and it is ensured that reactants can be fully converted.

The utility model provides a high-strength plastic fan blade, which relates to the technical field of fan blade components and comprises a fixing mechanism, the fixing mechanism comprises a connecting rod, a protection mechanism is arranged on the surface of one end of the connecting rod, a base is arranged on the surface of the connecting rod, two limiting rings are connected to the surface of the base, and fan blades are arranged between the two limiting rings. According to the utility model, through the installed fixing mechanism, after the screw thread is fixed, the base and the fixing shell also form limiting, the first gasket can enable one end of the fixing piece with the rotating handle to be more tightly attached to the fixing piece, sliding is avoided, movement of the fixing piece caused by shaking is avoided, and limiting can be formed by rotating the fixing piece and performing screw thread fixing with the threaded pipe. Once the screw thread is fixed, the base and the fixed shell are limited, so that a stable fixed state is ensured. The multiple limiting fixing mode can effectively prevent the fan blades from shaking and moving, and the fixing reliability is improved.

The invention discloses a preparation method of a pilose antler mushroom extract and application of the pilose antler mushroom extract in products with nitrite adsorption and / or lipid lowering efficacy, SDF in pilose antler mushroom is extracted, it is found that the content of SDF in pilose antler mushroom is high, the pilose antler mushroom has good in-vitro lipid lowering activity, and the pilose antler mushroom is a high-quality edible mushroom raw material capable of being used for lipid lowering functional food development. A biological activity experiment shows that the pilose antler mushroom SDF extract can well remove DPPH and ABTS free radicals, adsorb nitrite and inhibit the activity of cholesterol esterase and pancreatic lipase, and shows good oxidation resistance, nitrite adsorption and lipid-lowering activity.

The invention provides a magnesium alloy surface high-wear-resistance micro-arc oxidation film layer and a preparation method thereof. The preparation method comprises the following steps: S1, pretreatment of a magnesium alloy matrix: grinding and polishing the magnesium alloy matrix, and then cleaning and drying the magnesium alloy matrix; s2, preparing a silicate alkaline electrolyte: dissolving Na2SiO3, KF and NaOH in deionized water at room temperature, performing ultrasonic treatment until the Na2SiO3, the KF and the NaOH are completely dissolved, then adding the TiO2 nano-particles, and continuing ultrasonic treatment until the TiO2 nano-particles are uniformly dispersed in the deionized water to obtain the silicate alkaline electrolyte; and S3, micro-arc oxidation treatment is conducted, specifically, stainless steel serves as a cathode, the pretreated magnesium alloy matrix serves as an anode, the cathode and the anode are put into a silicate alkaline electrolyte, flushing and drying are conducted after micro-arc oxidation treatment, and then the high-abrasion-resistance micro-arc oxidation film layer is formed on the magnesium alloy sample. The structure, the structure and the performance of the magnesium alloy micro-arc oxidation film layer are improved by adding the nano TiO2 particles into the electrolyte, so that the wear resistance of the film layer is improved.

The invention discloses combined lactobacillus salivarius CCFM1398 for regulating and controlling bone homeostasis and relieving bone resorption and a metagen of the combined lactobacillus salivarius CCFM1398, and belongs to the technical field of microorganisms. The combined lactobacillus salivarius CCFM1398 provided by the invention is separated from oral dental plaque of healthy teenagers, has the effects of relieving bone resorption and resisting inflammation, and is specifically reflected in that expression of protein level and gene level of MC3T3-E1 cell osteoprotegerin (OPG) is improved in vitro; the bone absorption amount of orthodontic bone absorption rat alveolar bone is reduced, the number of alveolar bone osteoclasts is reduced, and the level of rat serum OPG is improved; the secretion of inflammatory factors TNF-alpha, IL-6 and MPO of the orthodontic bone absorption rat is reduced; and the pathological condition of orthodontic bone absorption rat alveolar bone tissue is improved. Therefore, the combined lactobacillus salivarius has a huge application prospect in products for relieving bone resorption and assisting in inhibiting periodontal tissue inflammation.

The present invention provides a chemical synthesis method of EPS-G7, wherein β-cyclodextrin A is acetylated to obtain β-acetylcyclodextrin B, B is ring-opened in a mixed solution of acetic anhydride / concentrated sulfuric acid to obtain a chain acetylated heptose C, p-nitrophenol is condensed with intermediate C using boron trifluoride acetonitrile to obtain a key intermediate p-nitrophenyl (G1)-O-acetyl-D-maltoseptaglycoside D with α as the dominant configuration, D is removed from the acetyl group under the action of sodium methoxide to obtain pNP-G7 (p-nitrophenyl (G1)-D-maltoseptaglycoside), pNP-G7 is in the presence of p-toluenesulfonic acid and dimethoxyethane, and acetal protection is formed between the hydroxyl groups on C4 and C6 of the terminal monosaccharide to obtain EPS-G7. The reaction raw materials used in the method of the present invention are cheap and readily available, and the chemical synthesis of α-amylase detection reagent EPS-G7 is realized, which is a simple, economical and efficient synthesis method.

The invention provides an anti-CD40 monoclonal antibody and application of the anti-CD40 monoclonal antibody. The anti-CD40 monoclonal antibody comprises three heavy chain complementarity determining regions (HCDR1, HCDR2 and HCDR3) as shown in SEQ ID NOs: 1-3, and three light chain complementarity determining regions (LCDR1, LCDR2 and LCDR3) as shown in SEQ ID NO: 4, YAS and SEQ ID NO: 5. The antibody has binding activity with in-vitro CD40 and CD40 on the cell surface, and can be competitively bound with CD40L to block binding of the antibody and CD40L so as to inhibit CD40 / CD40L signal transduction and inhibit B cell proliferation and activation, so that immune overexpression and rejection can be inhibited. Therefore, when the antibody is applied to preparation of drugs for treating immune diseases, the antibody has the advantages of high targeting specificity, high affinity, few side effects and the like, and has a great application prospect.