468 results about "Phenolic acid" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus mobilis DG-7 and application thereof in inhibiting bolting of angelica sinensis and improving disease resistance of angelica sinensis. The invention provides a strain of bacillus mobilis DG-7, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.31089. The bacillus mobilis DG-7 and the microbial inoculum thereof provided by the invention can play roles in inhibiting early bolting of angelica sinensis and improving disease resistance by increasing the content of plant endogenous melatonin and salicylic acid, reducing the content of abscisic acid, auxin and gibberellin, reducing the content of total phenolic acid and reducing the expression of photoperiod-related genes. In addition, the bacillus mobilis DG-7 does not solubilize phosphorus, so that the promotion effect of high-concentration phosphorus on early bolting is effectively avoided; the laccase activity of the bacillus mobilis DG-7 is high, polyphenol substances can be effectively removed, and the bolting rate can be reduced.

The invention discloses bacillus velezensis YB-1652, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum, and aims to solve the technical problem of lack of effective biological control measures for continuous accumulation of peanut phenolic acid autotoxic substances and pathogens in continuous cropping. According to the application, a bacillus velezensis YB-1652 strain is separated and screened from rhizosphere soil of continuous cropping peanuts, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36152. The strain has the capability of efficiently degrading phenolic acid compounds, relieves continuous cropping obstacles and improves the soil environment; the compound has a relatively strong inhibition effect on southern blight and root rot of peanuts, and the occurrence rate of soil-borne diseases is reduced; meanwhile, peanut growth can be promoted; the bacillus amyloliquefaciens has siderophore, cellulase, beta-glucanase, amylase power and protease production capacity. The YB-1652 strain and the preparation thereof can provide technical support for restoration of peanut continuous cropping obstacles, provide bacterial source guarantee for prevention and treatment of peanut soil-borne diseases, replace or reduce use of chemical pesticides and improve food and ecological environment safety, and have good economic and social benefits.

The invention belongs to the technical field of microorganisms, and particularly relates to pseudomonas monteilii BDLZ-3 and application thereof in relieving continuous cropping obstacles of crops. The invention discloses a strain of pseudomonas monteilii BDLZ-3, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.34349. The pseudomonas monteilii BDLZ-3 is separated and screened from the nature for the first time, phenolic acid autotoxic substances generated in the ginseng continuous cropping obstacle process can be efficiently degraded, the degradation rate of benzoic acid can reach 95.36%, various phenolic acids such as vanillic acid, cinnamic acid, palmitic acid and gallic acid can be effectively degraded, and the degradation spectrum is wide; when the pseudomonas monteilii BDLZ-3 is applied in a field, the content of benzoic acid in soil can be effectively reduced, meanwhile, the occurrence of ginseng root diseases can be controlled to a certain extent, the prevention and treatment effect reaches 41.23%, the growth indexes such as root length and root thickness are remarkably improved, and the yield increasing effect is achieved.

The invention provides a content detection method for a salvia miltiorrhiza medicinal material, a salvia miltiorrhiza clear paste and a musk heart-dredging dropping pill. According to the method, a high performance liquid chromatography method is adopted, octadecylsilane chemically bonded silica is used as a chromatographic column filler, acetonitrile is used as a mobile phase A, 0.02%-0.1% trifluoroacetic acid is used as a mobile phase B, gradient elution is carried out, and rosmarinic acid and salvianolic acid B in the salvia miltiorrhiza medicinal material are synchronously and quantitatively detected. And synchronously and quantitatively detecting tanshinol, protocatechuic aldehyde, caffeic acid, salvianolic acid E, rosmarinic acid, alkannic acid and salvianolic acid B in the salvia miltiorrhiza clear paste, and synchronously and quantitatively detecting tanshinol, protocatechuic aldehyde, caffeic acid, salvianolic acid E, rosmarinic acid, alkannic acid, salvianolic acid B, arenobufagin and bufalin in the musk Tongxin dropping pill. The method is used for monitoring the content of specific components, can also judge the transfer rate in the medicine production process, and facilitates traceability in the production link. In addition, the method is relatively low in equipment requirement and suitable for industrial popularization.

The invention discloses a special controlled-release nitrogen fertilizer for rice containing a nitrogen signal substance and application of the special controlled-release nitrogen fertilizer. The special controlled-release nitrogen fertilizer containing the nitrogen signal substance for the rice sequentially comprises nitrogen fertilizer particles, a signal substance layer and a coating layer from inside to outside, the nitrogen fertilizer particles are quick-acting nitrogen fertilizer; the signal substance layer is a phenolic acid-beta-cyclodextrin inclusion compound; the preparation method comprises the following steps: completely dissolving beta-cyclodextrin in distilled water, heating, slowly dropwise adding an ethanol solution containing phenolic acid, a chelating agent and an antioxidant while stirring, and reacting to obtain the phenolic acid-beta-cyclodextrin inclusion compound. And the coating layer is prepared from bio-based polyol and isocyanate. According to the invention, phenolic acid is used as a signal substance, beta-cyclodextrin is added to include phenolic acid, and an antioxidant and a chelating agent are added to coat the surface of urea to prepare nitrogen fertilizer particles; and coating a coating material to obtain the special controlled-release nitrogen fertilizer containing the nitrogen signal substance for the rice. The controlled-release fertilizer not only can accurately release the nitrogen fertilizer and reduce the waste of the nitrogen fertilizer, but also can improve the yield and quality of the rice and reduce common diseases of the rice.

The invention relates to the technical field of cosmetic functional raw material preparation methods, in particular to a plant extract and an extraction method and application thereof. The invention provides a plant extraction method, which comprises the following steps: adding a glycol ether or polyhydric alcohol solvent containing 5-7 carbon atoms into a product containing phenolic acid compound components and lignan compound components, carrying out homogeneous reaction, carrying out heating extraction, and collecting the extract. By adopting a supramolecular technology, phenolic acid compound components can form a supramolecular solvent with glycol ether or polyol solvents containing 5-7 carbon atoms, and the supramolecular solvent is further combined with lignan compound components to form a supramolecular structure, so that the stability of an extracting solution is remarkably enhanced, the specific enrichment of the lignan compound components is realized, and the specific enrichment of the lignan compound components is realized. The problems that an existing extraction technology is poor in stability, poor in formula applicability, not prominent in effect and the like are successfully solved.

The invention discloses streptomyces bulbus as well as a multifunctional fungicide and application thereof. According to the invention, firstly, a strain of Streptomyces tauricus zhanshen-LZ-12 is separated and screened from straw returning soil, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.34456. The invention also discloses a preparation method of the Streptomyces tauricus zhanshen-LZ-12. The strain has the functions of degrading cellulose, decomposing protein, fixing nitrogen, resisting salt, degrading phenolic acid autotoxic substances (benzoic acid, p-hydroxybenzoic acid and cinnamic acid) and the like. Bacterial powder obtained after fermentation of the bacterial strain is uniformly mixed with bacillus bacterial powder to obtain the multifunctional bacterial agent, and when straw returning or composting is carried out, the multifunctional bacterial agent is adopted, straw decomposition can be accelerated, soil ecology can be improved, crop growth can be promoted, and the stress resistance of crops can be enhanced.

The invention relates to the technical field of energetic materials, in particular to a laser response type high-entropy alloy-based initiating explosive material and a preparation method thereof. The initiating explosive material is mainly formed by uniformly loading a heat-sensitive high-energy compound (such as lead stilbeneate or hexa (4-amino-3, 5-dinitropyrazole) copper) on high-entropy alloy nanoparticles containing five or more metal elements, wherein the heat-sensitive high-energy compound is such as lead stilbeneate or hexa (4-amino-3, 5-dinitropyrazole) copper. The preparation method comprises the following steps: mixing acetylacetone metal salt, a surfactant and a reducing agent in an organic solvent, and stirring and reacting at 180-220 DEG C to prepare high-entropy alloy nanoparticles; the nano particles and a high-energy compound are co-dispersed in a mixed solvent containing a dispersing agent, ultrasonic treatment is carried out to form suspension liquid, and freeze drying is carried out to obtain the nano particles. The method is characterized in that the excellent photo-thermal conversion performance of the high-entropy alloy is utilized, under low laser energy irradiation, a high-energy compound can be effectively excited, and stable detonation of secondary charge CL-20 is successfully achieved. According to the invention, the problems of high response threshold and low output energy of the existing laser initiating explosive material are solved.

The invention provides a combined lactobacillus salivarius YS-UR90 and a preparation method of the combined lactobacillus salivarius YS-UR90. The saliva combined lactobacillus provided by the invention can secrete protease and glycosidase, and the HCT and FLS gene expression levels of the saliva combined lactobacillus are high, so that the saliva combined lactobacillus can promote phenolic acid active ingredients to generate biotransformation during fermentation, and the proportion of chlorogenic acid and the like in fermentation liquor is increased. For example, marine fish oligopeptide and casein phosphopeptides are used as a composite nitrogen source, dandelion powder is used as a functional substrate, and a composite fermentation product obtained through fermentation of saliva and lactobacillus has the effect of promoting uric acid excretion and has a certain protection effect on kidney injury. The compound fermentation product has the effect of improving hyperuricemia mainly by inhibiting XOD activity, increasing serum urea nitrogen level, improving defect expression of uric acid synthesis gene HPRT1, inhibiting expression of uric acid reabsorption URAT1 and GLUT9 genes and improving expression of excretion genes OAT1 and ABCG2.

The invention belongs to the technical field of natural medicines, and particularly discloses a lipase inhibitor with a specific structure as well as a preparation method and application thereof, the inhibitor is derived from grape leaves and contains C6-C3-C6, 1, 2-benzopyrone, diphenyl ethylene and a phenolic hydroxyl structure, and the lipase inhibitor is a lipase inhibitor with a specific structure. Comprising the following components in percentage by mass: 72.1 to 97.5 percent of flavonoids, 2.5 to 5.1 percent of coumarins, 2.0 to 3.2 percent of stilbenes and 5.1 to 8.3 percent of phenolic acids. According to the lipase inhibitor with the specific structure as well as the preparation method and the application of the lipase inhibitor, the inhibitor can inhibit secretion of lipase, prevent fat from being decomposed into absorbable free fatty acid and monoacylglycerol, and reduce fat absorption; the total cholesterol and the low-density lipoprotein cholesterol are reduced, the blood fat level is improved, and the composition can be applied to preparation of health-care products, functional foods, sports nutritious foods and formula foods for special medical purposes.

The invention discloses application of salvianolic acid A in preparation of a medicine for preventing and treating vulnerable plaques of advanced atherosclerosis. The invention also discloses application of the salvianolic acid A combined with ferulic acid or the salt thereof in preparation of drugs for preventing and treating advanced atherosclerosis vulnerable plaques. Researches find that salvianolic acid A can promote apoptosis of senescent vascular smooth muscle cells and has a Senlytics effect; the salvianolic acid A stabilizes vulnerable atherosclerotic plaques by reducing the load of senescent vascular smooth muscle cells; the salvianolic acid A is in non-covalent binding with ASP54, ALA55, LYS58, ASP93, ASP102 and LYS112 of the HSP90AA1 to play a role in inhibition, and the ASP93 has the greatest contribution to binding free energy. The research of the invention is of great significance to the development and application of drugs with anti-aging effect for stabilizing advanced atherosclerotic vulnerable plaques.

Preparation of cannabinoid formulations containing: Δ9-tetrahydrocannabinol (Δ9-THC), Δ8-tetrahydrocannabinol (Δ8-THC), Δ9-tetrahydrocannabinolic acid (THCa), cannabidiol (CBD), cannabidiolic acid (CBDa), cannabigero (CBG), cannabichromene (CBC) and cannabinol (CBN), either alone or in combinations henceforth known as cannabis, have been created using an emulsification process to encapsulate cannabinoids. The aqueous-based method involves micellular encapsulation of cannabinoids, a method that has been used to increase the bioavailability of poorly permeable, lipophilic drugs. The present invention demonstrates the viability of transdermal delivery with gels and patches for consistent and sustained cannabinoid dosing.

The invention discloses a nanometer emulsion based on a citrus extract and a preparation method of the nanometer emulsion. The nano-emulsion takes citrus pectin as a main chain, gallic acid, ascorbic acid-phosphate and oleic acid are sequentially grafted, the oleic acid is lipophilic, the rest is hydrophilic, and the amphiphilic pectin can form the whitening nano-emulsion which can be stably stored for a long time. The natural citrus extract is used for replacing traditional synthetic components, has the anti-oxidation, whitening and anti-inflammatory effects, can reduce the skin sensitivity risk, and conforms to the green beauty makeup trend; meanwhile, the oil phase is anchored by a hydrophobic block, steric hindrance is formed by a hydrophilic block, the stability of the emulsion is remarkably improved, and the shelf life is prolonged to 24 months; phenolic acid and ascorbic acid contained in the traditional Chinese medicine composition can effectively scavenge free radicals, inhibit tyrosinase activity, reduce melanogenesis and reduce release of a proinflammatory factor IL-6; the composition is low in cost, environment-friendly, suitable for the fields of cosmetics and medicines and wide in market prospect.

The invention relates to the technical field of traditional Chinese medicine detection, in particular to a characteristic spectrum construction method, a characteristic spectrum and a detection method of traditional Chinese medicine granules. The traditional Chinese medicine granules comprise protocatechuic aldehyde, hydroxysafflor yellow A, salvianolic acid B, cinnamic acid and cinnamaldehyde; the construction method comprises the following steps: taking protocatechuic aldehyde, hydroxysafflor yellow A, salvianolic acid B, cinnamic acid and cinnamaldehyde reference substances, and preparing a mixed reference substance solution; preparing a test solution from the traditional Chinese medicine granules; and respectively carrying out liquid chromatographic analysis on the mixed reference substance solution and the test solution to obtain the characteristic chromatogram of the traditional Chinese medicine granules. Wherein the liquid chromatographic analysis conditions comprise that a phosphoric acid solution is used as a mobile phase A, acetonitrile is used as a mobile phase B, and gradient elution is carried out. The problem that the quality of traditional Chinese medicine particles is difficult to control is solved.

The invention belongs to the field of biological pharmacy, and particularly relates to a phenolic acid composition and application thereof in promoting digestion. According to the invention, monophenolic acid for improving dyspepsia is found through a single-component phenolic acid experiment, and then a phenolic acid compound with a synergistic effect is searched in a compounding manner, so that unexpectedly, the combination of two phenolic acids with the best digestion promoting effect cannot realize the effect optimization; on the contrary, syringic acid with the worst digestion promoting effect is compounded with other phenolic acids, so that the synergistic digestion promoting effect is achieved, the effect of a positive medicine can be achieved, and a potential optional medicine is provided for improving dyspepsia.

The present application relates to cannabidiol esters, compositions comprising them and their use in the treatment of a variety of different diseases, disorders and symptoms.

The invention discloses a construction method and application of a specific chromatogram of a Shanhaidan pharmaceutical preparation, and belongs to the technical field of traditional Chinese medicine detection. The method comprises the following steps: preparing a test solution by using a plurality of different batches of Shanhaidan medicinal preparations in combination with high performance liquid chromatography and mass spectrometry technologies; the preparation method comprises the following steps: preparing a single reference substance solution from uridine, tanshinol, daidzin, p-hydroxybenzoic acid, puerarin, caffeic acid, 3 '-hydroxy puerarin, physcion, calycosin-7-O-beta-D-glucoside, stilbene glucoside, rosmarinic acid, alkannic acid, formononin, salvianolic acid B, daidzein and calycosin-7-one; and respectively carrying out high performance liquid chromatography analysis on the test solution and the reference solution to obtain the characteristic chromatogram of the Shanhaidan pharmaceutical preparation. The method is simple and convenient to operate, high in precision, good in reproducibility and accurate and reliable in result. And at least 16 components can be identified at one time under the same chromatographic condition, so that the detection efficiency is remarkably improved, the time and the cost are saved, and the quality of the Shanhaidan pharmaceutical preparation is comprehensively and scientifically evaluated.

The invention discloses application of dikafen phenolic acid and ester derivatives thereof in preparation of medicines for treating pulmonary fibrosis. On an MRC-5 cell model induced by TGF-beta1, the bikafen phenolic acid and the multiple ester derivatives thereof show the activity of inhibiting alpha-SMA and COL1A1 protein expression; on a bleomycin-induced pulmonary fibrosis mouse model, the dimethyl ester derivative of the dikafen phenolic acid shows a good protective effect on experimental animals. Therefore, the dicafen phenolic acid and the ester derivative thereof have good application in the field of preparation of medicines for treating pulmonary fibrosis diseases.

The invention discloses a bacillus velezensis strain and application thereof. The invention discloses bacillus velezensis WLH (bacillus velezensis), and the preservation number of the bacillus velezensis WLH is CCTCC (China Center for Type Culture Collection) NO: M 20251238. The bacillus velezensis WLH is screened from rhizosphere of cucumber, the WLH is a biocontrol bacterium, can inhibit growth of pathogenic bacteria of cucumber root rot and fusarium wilt by degrading phenolic acid in soil and promote growth of cucumber plants, and the strain can relieve autotoxicity caused by the phenolic acid, inhibit the cucumber root rot and fusarium wilt and promote growth of cucumber plants. The promotion of crop growth comprises the capability of producing indoleacetic acid (IAA) and the capability of solubilizing phosphate, the promotion of germination of cucumber seeds under the stress of mixed phenolic acid and pathogenic bacteria, and the promotion of growth of cucumber seedlings under the stress of mixed phenolic acid and pathogenic bacteria. Soil continuous cropping obstacles generated in cucumber planting are effectively relieved, and a new basis is provided for microbial control of continuous cropping obstacles.

The application discloses a method for simultaneously determining phenolic acid and tanshinone components in Danshen injection, and according to the structure and property characteristics of the phenolic acid and tanshinone components, the best mobile phase composition, elution program, flow rate, chromatographic column and mass spectrometry condition are screened through a large number of experiments. Experimental verification shows that the UPLC-TQ-MS / MS method can simultaneously determine 11 different structure and type of phenolic acid and tanshinone component compounds, and the methodological verification shows that the detection method has good precision, repeatability and high accuracy, and has important significance for controlling the quality of Danshen injection and guaranteeing the clinical curative effect.

The invention provides a composition for inhibiting tumor growth and a preparation method thereof, and belongs to the technical field of medicines. After selenium is loaded, folic acid is coupled, and folic acid-selenium polysaccharide nanoparticles are prepared; notoginsenoside, curcumin, alizarin and hairyvein agrimony phenolic acid are embedded in pH-sensitive lipidosome, and the pH-sensitive lipidosome is uniformly mixed with folic acid-selenium polysaccharide nanoparticles and semicarbazone compounds to prepare the composition for inhibiting tumor growth. The composition for inhibiting tumor growth prepared by the invention has a relatively good anti-tumor effect, can release active components in a targeted manner at a tumor part, can reverse drug resistance, reduce the dosage of chemical drugs, reduce toxicity and improve patient compliance, is high in bioavailability, and has a wide application prospect.

A probiotic and prebiotic-embedded synbiotic system for targeted delivery and colonization of functions, comprising a probiotic core; an inner layer of protein-polysaccharide / oligosaccharide complex wrapped around the probiotic surface, which acts as both an adhesion promoter, promoting the attachment of probiotics to the intestinal epithelial cells, and as a prebiotic, promoting the growth of probiotics upon release; and an outer layer of a natural emulsifying system on the synbiotic complex, which minimizes water infiltration, thereby effectively embedding and protecting the probiotics. The present synbiotic system is suitable for production in powder form using freeze-drying technology, and is suitable as a dietary supplement or for addition to any food in dry powder form. The system has the ability to bioconvert soy isoflavones and release bioactive phenolic acids from grains to promote body health.

The invention relates to the technical field of forest cultivation, and discloses a carya illinoensis fruit forest variety configuration method which comprises the following steps: configuring a main cultivation variety and a pollination variety in a fruit forest; preparing a pollination synergistic composition; the composition is sprayed at the initial stage of stigma exposure of female flowers of a main cultivated variety. The composition comprises a phenolic acid grafted carboxymethyl chitosan derivative, low methoxyl pectin, a calcium ion cross-linking agent, sugar alcohol chelated boron and spermidine. According to the method, an interfacial film with antioxidant activity is formed on the surface of the stigma by using the grafting modified matrix, so that the stigma browning is effectively inhibited, and the pollination period is prolonged; by constructing a calcium cross-linked low-methoxyl pectin interpenetrating network, an interfacial film is endowed with an intelligent degradation characteristic of enzyme-sensitive response to pollen, and protection at ordinary times and immediate dissolution after touching flowers are realized. According to the method, pollen is captured through static electricity, nutritional factors are released in a targeted mode, the pollination and fertilization efficiency is remarkably improved, the sterility risk in the flowering phase is relieved, and the yield and quality of carya illinoensis are improved.

The invention provides a bacillus aerophilus Sys-Gs-010 strain capable of efficiently degrading benzoic acid in soil, the strain is preserved in the China Center for Type Culture Collection on December 10, 2025, the preservation number is CCTCC NO: M20252819, the preservation number is CCTCC NO: M20252819, the preservation number is CCTCC NO: M20252819, and the preservation number is CCTCC NO: M20252819. The 16S rDNA (ribosomal deoxyribonucleic acid) sequence of the strain is as shown in SEQ ID NO. 1. The bacillus aerosus Sys-Gs-010 strain provided by the invention can efficiently degrade ginseng to produce a main phenolic acid allelochemical-benzoic acid, and the strain is a non-ginseng pathogenic strain, can be applied to preparation of a ginseng allelochemical-benzoic acid degrading bacterial agent, greatly improves continuous cropping obstacles of ginseng, and has a good application prospect in the field of ginseng planting.

The present invention relates to the field of medical technology, and specifically to the use of salvianolic acid B in the preparation of drugs for treating IPEX. The present invention proves through in vivo animal experiments that salvianolic acid B has a good anti-inflammatory effect and can effectively alleviate the autoimmune reaction of Treg-deficient SF mice. In the present invention, the cations in the salvianolic acid B salts can be iron ions, calcium ions, sodium ions, potassium ions or magnesium ions, preferably magnesium ions; the dosage form of the drug for treating IPEX can be any dosage form described in pharmacy, and can be an oral liquid form, an injection form, a tablet form or a capsule form. By utilizing the key role of salvianolic acid B in IPEX disease, it can be prepared into a drug, providing a theoretical basis and a new treatment idea for the treatment of primary immunodeficiency diseases with traditional Chinese medicine; salvianolic acid B is easy to obtain, can be quickly put into production and obtain a high-efficiency preparation, and has important application value.

The application discloses a use of a pharmaceutical composition of a phenolic acid compound and probiotics, and particularly relates to a combination of chlorogenic acid and lactobacillus reuteri. The combination of chlorogenic acid and lactobacillus reuteri can improve glycolipid metabolic disorder, improve cognitive dysfunction, emotional disorder and delay aging, and can be used for preparing a medicine for treating metabolic disorder combined with neuropsychiatric diseases.

The invention discloses multifunctional double-drug co-loaded nanoparticles as well as a preparation method and application thereof, and belongs to the technical field of medicines. A Janus nano material composed of mesoporous silica and mesoporous polydopamine is respectively loaded with salvianolic acid B and polymyxin B under the condition of pH 4.5 to obtain the double-drug co-loaded nano-particles, the double-drug co-loaded nano-particles have good biocompatibility and synergistic treatment effect, and the double-drug co-loaded nano-particles can reduce active oxygen accumulation and inhibit oxidative stress and inflammatory response, so that the double-drug co-loaded nano-particles can be used for preparing the anti-inflammatory drug for treating the salvianolic acid B and the polymyxin B. The endothelial injury caused by sepsis is effectively relieved. The salvianolic acid B and the polymyxin B are loaded at the same time, the salvianolic acid B protects endothelial cells by removing active oxygen and reducing inflammatory reaction, the polymyxin B kills gram-negative bacteria by destroying bacterial cell membranes and reduces infection sources and endotoxin release, and through the synergistic effect of the salvianolic acid B and the polymyxin B, the blood vessel leakage can be reduced, and the blood vessel leakage can be reduced. And the integrity of the endothelial barrier can be enhanced, so that the aim of treating sepsis endothelial injury is fulfilled.

The invention discloses application of selfheal total phenolic acid in preparation of a medicine for improving an insomnia sleep structure. The spica prunellae total phenolic acid can effectively shorten the awakening duration, remarkably prolong the rapid eye movement sleep duration, improve the sleep continuity and the like to prolong the total sleep duration and adjust the sleep structure, so that the autonomous activity state and the learning and exploration ability of a patient are recovered. After the total phenolic acid of prunella vulgaris is used for treating insomnia, the sleep duration is gradually prolonged, and no abstinence reaction is generated.

The invention discloses a process method for optimizing total flavone and total phenolic acid extraction of gendarussa ventricosa based on a multi-index comprehensive scoring method in combination with a response surface method. A single factor experiment is carried out by taking the material-liquid ratio, the extraction temperature, the extraction time and the ethanol concentration as investigation factors, the total flavone content and the total phenolic acid content are taken as response values, the extraction process is optimized through a response surface design test, and the optimal extraction process of the total flavone of the gendarussa vulgaris is obtained: the material-liquid ratio is 1: 30, the extraction temperature is 60 DEG C, the extraction time is 45 minutes, the ethanol concentration is 70%, and the total flavone content is 2.487 + / -0.1332 mg / g; according to the optimal extraction process of the total phenolic acid, the material-liquid ratio is 1: 20, the extraction temperature is 55 DEG C, the extraction time is 40 min, the ethanol concentration is 70%, and the content of the obtained total phenolic acid is 10.625 + / -0.285 mg / g. The process method provided by the invention is stable, feasible, simple and easy to operate, good in repeatability and relatively high in extraction efficiency.

The invention relates to a non-natural cannabinoid synthase comprising at least one amino acid variation as compared to a wild type cannabinoid synthase Δ9-tetrahydrocannabinolic acid synthase (THCAS), comprising three alpha helices (αA, αB and αC) where a disulfide bond is not formed between alpha helix αA and alpha helix αC, wherein the non-natural cannabinoid synthase catalyzes the oxidative cyclization of cannabigerolic acid (CBGA) into a cannabinoid. The invention further relates to a non-natural Δ9-tetrahydrocannabinolic acid synthase (THCAS), a non-natural cannabidiolic acid synthase (CBDAS), and a non-natural cannabichromenic acid synthase (CBCAS) comprising at least one amino acid variation as compared to a wild type THCAS, CBDAS, or CBCAS, respectively, comprising three alpha helices (αA, αB and αC) and wherein a disulfide bond is not formed between alpha helix αA and alpha helix αC. The invention also relates to a nucleic acid, expression construct, and engineered cell for making the non-natural THCAS, CBDAS, and / or CBCAS. Also provided are compositions comprising the non-natural THCAS, CBDAS, and / or CBCAS; isolated non-natural THCAS, CBDAS, and / or CBCAS enzymes; methods of making the isolated enzymes; cell extracts comprising cannabinoids; and methods of making cannabinoids.