596 results about "Phenolic acid" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus mobilis DG-7 and application thereof in inhibiting bolting of angelica sinensis and improving disease resistance of angelica sinensis. The invention provides a strain of bacillus mobilis DG-7, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.31089. The bacillus mobilis DG-7 and the microbial inoculum thereof provided by the invention can play roles in inhibiting early bolting of angelica sinensis and improving disease resistance by increasing the content of plant endogenous melatonin and salicylic acid, reducing the content of abscisic acid, auxin and gibberellin, reducing the content of total phenolic acid and reducing the expression of photoperiod-related genes. In addition, the bacillus mobilis DG-7 does not solubilize phosphorus, so that the promotion effect of high-concentration phosphorus on early bolting is effectively avoided; the laccase activity of the bacillus mobilis DG-7 is high, polyphenol substances can be effectively removed, and the bolting rate can be reduced.

The invention discloses bacillus velezensis YB-1652, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum, and aims to solve the technical problem of lack of effective biological control measures for continuous accumulation of peanut phenolic acid autotoxic substances and pathogens in continuous cropping. According to the application, a bacillus velezensis YB-1652 strain is separated and screened from rhizosphere soil of continuous cropping peanuts, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36152. The strain has the capability of efficiently degrading phenolic acid compounds, relieves continuous cropping obstacles and improves the soil environment; the compound has a relatively strong inhibition effect on southern blight and root rot of peanuts, and the occurrence rate of soil-borne diseases is reduced; meanwhile, peanut growth can be promoted; the bacillus amyloliquefaciens has siderophore, cellulase, beta-glucanase, amylase power and protease production capacity. The YB-1652 strain and the preparation thereof can provide technical support for restoration of peanut continuous cropping obstacles, provide bacterial source guarantee for prevention and treatment of peanut soil-borne diseases, replace or reduce use of chemical pesticides and improve food and ecological environment safety, and have good economic and social benefits.

The invention provides a coating preservation material as well as a preparation method and application thereof, and belongs to the technical field of chemical modification of biomass organic polymer materials. The structural formula of the chitosan / polyphenol / quaternary phosphonium salt derivative disclosed by the invention is as shown in # imgabs0 #. The preparation method comprises the following steps: modifying chitosan by using natural phenolic acid and quaternary phosphonium salt to prepare an amphiphilic chitosan derivative; then preparing a plant essential oil microcapsule by taking the amphiphilic chitosan derivative as a wall material and plant essential oil as a core material; and finally, compounding the plant essential oil microcapsules with a film-forming material to construct a film-coating fresh-keeping material, and applying the film-coating fresh-keeping material to fresh keeping of fruits and vegetables.

The invention relates to a preparation method of a silver nanowire based on a phenolic compound and the silver nanowire, and the preparation method comprises the following steps: 1, respectively dissolving polyvinylpyrrolidone, small molecular phenolic acid, halide, a silver source and the phenolic compound in deionized water to obtain respective solutions; 2, sequentially adding a polyvinylpyrrolidone solution, a small molecular phenolic acid solution, a halide solution and a silver source solution, and stirring to obtain a reaction solution A; step 3, adding a phenolic compound solution into the reaction solution A to obtain a reaction system B, and stirring and reacting to obtain a mixed solution C containing silver nanowires; fourthly, the mixed solution C containing the silver nanowires is sequentially centrifuged and washed, and then the silver nanowires are prepared; the diameter of the silver nanowire is 20-150 nm, and the length of the silver nanowire is 5-100 [mu] m. Therefore, according to the design, water is adopted as a solvent to synthesize the silver nanowire, the reaction temperature is low, and the reaction speed is high.

The invention belongs to the technical field of biology, and particularly relates to a difunctional gene cluster biosynthesized by phenol amine and salicylic acid and application of the difunctional gene cluster. The gene cluster comprises five genes, the five genes are S1EPS1, S1PBS3, S1CoAOMT2, S1CoOOMT3 and S1CoAOMT4, the nucleotide sequence of the S1EPS1 gene is as shown in SEQ ID No.1, the nucleotide sequence of the S1PBS3 gene is as shown in SEQ ID No.3, the nucleotide sequence of the S1CoAOMT2 gene is as shown in SEQ ID No.5, the nucleotide sequence of the S1CoOOMT3 gene is as shown in SEQ ID No.7, and the nucleotide sequence of the S1CoAOMT4 gene is as shown in SEQ ID No.9. The invention further discloses a preparation method of the gene cluster. The overexpressed gene cluster component SlEPS1 gene can significantly enhance accumulation of spermidine derivatives and salicylic acid of tomato plants so as to enhance the resistance to Pst DC3000, lays a foundation for genetic improvement in the aspect of tomato disease resistance by using the gene cluster, and also provides technical reference for excavation of related metabolic gene clusters in other crops and in the aspect of resistance breeding.

The invention belongs to the technical field of microorganisms, and particularly relates to pseudomonas monteilii BDLZ-3 and application thereof in relieving continuous cropping obstacles of crops. The invention discloses a strain of pseudomonas monteilii BDLZ-3, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.34349. The pseudomonas monteilii BDLZ-3 is separated and screened from the nature for the first time, phenolic acid autotoxic substances generated in the ginseng continuous cropping obstacle process can be efficiently degraded, the degradation rate of benzoic acid can reach 95.36%, various phenolic acids such as vanillic acid, cinnamic acid, palmitic acid and gallic acid can be effectively degraded, and the degradation spectrum is wide; when the pseudomonas monteilii BDLZ-3 is applied in a field, the content of benzoic acid in soil can be effectively reduced, meanwhile, the occurrence of ginseng root diseases can be controlled to a certain extent, the prevention and treatment effect reaches 41.23%, the growth indexes such as root length and root thickness are remarkably improved, and the yield increasing effect is achieved.

The invention provides a content detection method for a salvia miltiorrhiza medicinal material, a salvia miltiorrhiza clear paste and a musk heart-dredging dropping pill. According to the method, a high performance liquid chromatography method is adopted, octadecylsilane chemically bonded silica is used as a chromatographic column filler, acetonitrile is used as a mobile phase A, 0.02%-0.1% trifluoroacetic acid is used as a mobile phase B, gradient elution is carried out, and rosmarinic acid and salvianolic acid B in the salvia miltiorrhiza medicinal material are synchronously and quantitatively detected. And synchronously and quantitatively detecting tanshinol, protocatechuic aldehyde, caffeic acid, salvianolic acid E, rosmarinic acid, alkannic acid and salvianolic acid B in the salvia miltiorrhiza clear paste, and synchronously and quantitatively detecting tanshinol, protocatechuic aldehyde, caffeic acid, salvianolic acid E, rosmarinic acid, alkannic acid, salvianolic acid B, arenobufagin and bufalin in the musk Tongxin dropping pill. The method is used for monitoring the content of specific components, can also judge the transfer rate in the medicine production process, and facilitates traceability in the production link. In addition, the method is relatively low in equipment requirement and suitable for industrial popularization.

The invention discloses a special controlled-release nitrogen fertilizer for rice containing a nitrogen signal substance and application of the special controlled-release nitrogen fertilizer. The special controlled-release nitrogen fertilizer containing the nitrogen signal substance for the rice sequentially comprises nitrogen fertilizer particles, a signal substance layer and a coating layer from inside to outside, the nitrogen fertilizer particles are quick-acting nitrogen fertilizer; the signal substance layer is a phenolic acid-beta-cyclodextrin inclusion compound; the preparation method comprises the following steps: completely dissolving beta-cyclodextrin in distilled water, heating, slowly dropwise adding an ethanol solution containing phenolic acid, a chelating agent and an antioxidant while stirring, and reacting to obtain the phenolic acid-beta-cyclodextrin inclusion compound. And the coating layer is prepared from bio-based polyol and isocyanate. According to the invention, phenolic acid is used as a signal substance, beta-cyclodextrin is added to include phenolic acid, and an antioxidant and a chelating agent are added to coat the surface of urea to prepare nitrogen fertilizer particles; and coating a coating material to obtain the special controlled-release nitrogen fertilizer containing the nitrogen signal substance for the rice. The controlled-release fertilizer not only can accurately release the nitrogen fertilizer and reduce the waste of the nitrogen fertilizer, but also can improve the yield and quality of the rice and reduce common diseases of the rice.

The invention relates to a microbial agent for preventing and treating root rot of radix tinosporae and application of the microbial agent. The microbial agent is prepared from the following components: bacillus velezensis with the preservation number of CCTCC (China Center for Type Culture Collection) No: M2022923, bacillus subtilis with the preservation number of CCTCC No: M20221207, a marigold extract, amino acid, humic acid and medium trace elements. When the microbial agent is applied to the planting process of radix tinosporae, the physicochemical property of radix tinosporae planting soil can be remarkably improved, the enzyme activity and the microbial diversity can be improved, the content of phenolic acid and fusarium toxin in the soil can be reduced, the disease index of radix tinosporae root rot caused by fusarium solani and pythium aphanidermatum can be further reduced, and finally prevention and control of radix tinosporae root rot can be achieved.

The invention discloses hydrophilic modified polysulfone resin with side carboxyl groups and a preparation method of the hydrophilic modified polysulfone resin, and relates to the technical field of polysulfone. According to the invention, desalted water is used as a salt-forming solvent to carry out a salt-forming reaction of the bisphenol monomer, after the salt-forming reaction is finished, the salt-forming agent of the system is reacted and alkalinity is weakened, and then an NMP solvent is added to carry out water sampling, so that interference of NMP decomposition on carboxyl in a salt-forming stage is reduced, and the proportion of diphenolic acid connected with a molecular chain is consistent with a feeding ratio; the formed hydrophilic modified polysulfone resin is more stable in component; meanwhile, before end capping, side chain carboxyl forms a stable coordination complex, so that the end capping can be performed on the diphenolic acid type hydrophilic polysulfone, the content of phenol end groups with poor thermal stability is greatly reduced, and the natural color can be kept during extrusion granulation.

The invention discloses a preparation method of a phenolic acid-based phosphorus-nitrogen flame retardant, the phenolic acid-based phosphorus-nitrogen flame retardant and application of the phenolic acid-based phosphorus-nitrogen flame retardant, and the preparation method comprises the following steps: S1, dissolving a phenolic acid compound in absolute ethyl alcohol, adding 9, 10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide, carrying out heating reaction, then cooling, carrying out reduced pressure distillation, washing with deionized water, filtering and drying to obtain a flame-retardant intermediate; and S2, dissolving the flame-retardant intermediate in the step S1 in an organic solvent, dropwise adding phosphonitrilic chloride trimer dissolved by the organic solvent, carrying out heating reaction, cooling, carrying out reduced pressure distillation, washing with deionized water, filtering, and drying to obtain the phenolic acid-based phosphorus-nitrogen flame retardant. According to the present invention, the natural renewable phenolic acid compound is adopted as the main raw material, the chemical structure of the phenolic acid compound contains phosphorus atoms of different valence states so as to simultaneously provide the condensed phase and gas phase flame retardation mechanism, and the structure contains the nitrogen element and the aromatic ring so as to be suitable for the efficient flame retardation treatment of the polylactic acid material, and the like.

1. A composition, which is an anthocyanin-based colorant composition comprising 50-90 mol-%, based on the total amount of anthocyanins, of pelargonidin-based anthocyanins, and wherein (i) >70 mol-% of all anthocyanins are acylated with at least one phenolic acid; and (ii) >20 mol-% of all anthocyanins are acylated with at least one hydroxycinnamic acid; and wherein the composition has a red color with a hue value H≥ in the L*C*h* color system in the range of 10-30, measured at an L*-value of (70.0±0.1) in a 0.1 mol / l trisodium citrate dihydrate buffer at pH 3 in a 1 cm-length quartz cell using Spectraflash 650 (Datacolor) in transmission mode under D65 illuminant 10 Deg. Also, the present invention relates to use of the above compositions as a food colorant.

The invention relates to the technical field of gene engineering and recombinase catalysis engineering, in particular to a recombinant strain for expressing phenolic acid decarboxylase as well as a construction method and application of the recombinant strain. According to the invention, a recombinant strain for expressing the phenolic acid decarboxylase PADCs is constructed by heterologous expression of a coding gene PADCs of the phenolic acid decarboxylase. The enzyme liquid of the recombinant strain for expressing the phenolic acid decarboxylase has remarkable catalytic performance, p-coumaric acid can be used as a substrate, the recombinant strain is used for inducing expression, the purified enzyme liquid is used for catalyzing decarboxylation reaction of p-coumaric acid, and p-hydroxystyrene is synthesized; meanwhile, the phenolic acid decarboxylase PADCs has a wide substrate spectrum, the 4-vinylphenol compound can be used as a substrate, bicarbonate is used as a carboxyl source, the freeze-dried whole cell of the recombinant strain is utilized to catalyze the 4-vinylphenol compound to carry out regioselective beta-carboxylation reaction, a series of phenolic acids with biological activity are synthesized by an enzymic method, and meanwhile CO2 is immobilized; and a huge application potential is provided for the utilization of CO2.

The invention relates to the technical field of cosmetic functional raw material preparation methods, in particular to a plant extract and an extraction method and application thereof. The invention provides a plant extraction method, which comprises the following steps: adding a glycol ether or polyhydric alcohol solvent containing 5-7 carbon atoms into a product containing phenolic acid compound components and lignan compound components, carrying out homogeneous reaction, carrying out heating extraction, and collecting the extract. By adopting a supramolecular technology, phenolic acid compound components can form a supramolecular solvent with glycol ether or polyol solvents containing 5-7 carbon atoms, and the supramolecular solvent is further combined with lignan compound components to form a supramolecular structure, so that the stability of an extracting solution is remarkably enhanced, the specific enrichment of the lignan compound components is realized, and the specific enrichment of the lignan compound components is realized. The problems that an existing extraction technology is poor in stability, poor in formula applicability, not prominent in effect and the like are successfully solved.

The invention relates to a microbial agent for preventing and treating the root knot nematode disease of angelica sinensis and application of the microbial agent. The microbial agent is prepared from the following components: bacillus velezensis with the preservation number of CCTCC (China Center for Type Culture Collection) No: M2022923, bacillus paraamycoides with the preservation number of CCTCC No: M2022924, a szechwan Chinaberry fruit extract, amino-oligosaccharin, fulvic acid and a medium trace element fertilizer. When the microbial agent is used in the angelica sinensis planting process, the root activity, the root enzyme activity, the plant enzyme activity and the proline content can be remarkably improved, and the nematode resistance of crops is improved; the pH, medium trace elements, enzyme activity and microbial diversity index in the soil are remarkably improved, the phenolic acid content of the soil is remarkably reduced, and the soil environment is improved; the Chinese angelica root-knot nematode disease prevention and treatment method has the advantages that the Chinese angelica root-knot nematode disease prevention and treatment effect is obvious, and the Chinese angelica root-knot nematode disease prevention and treatment method has a wide application prospect. The Chinese angelica root-knot nematode disease prevention and treatment method can be used for preventing and treating the Chinese angelica root-knot nematode disease and preventing and treating the Chinese angelica root-knot nematode disease.

The invention discloses streptomyces bulbus as well as a multifunctional fungicide and application thereof. According to the invention, firstly, a strain of Streptomyces tauricus zhanshen-LZ-12 is separated and screened from straw returning soil, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.34456. The invention also discloses a preparation method of the Streptomyces tauricus zhanshen-LZ-12. The strain has the functions of degrading cellulose, decomposing protein, fixing nitrogen, resisting salt, degrading phenolic acid autotoxic substances (benzoic acid, p-hydroxybenzoic acid and cinnamic acid) and the like. Bacterial powder obtained after fermentation of the bacterial strain is uniformly mixed with bacillus bacterial powder to obtain the multifunctional bacterial agent, and when straw returning or composting is carried out, the multifunctional bacterial agent is adopted, straw decomposition can be accelerated, soil ecology can be improved, crop growth can be promoted, and the stress resistance of crops can be enhanced.

The invention provides a traditional Chinese medicine composition and application thereof, the traditional Chinese medicine composition contains total salvianolic acid and total triterpenic acid of hawthorn, and the traditional Chinese medicine composition has an excellent treatment effect on prevention or treatment of cerebrovascular diseases, especially ischemic cerebrovascular diseases.

The invention relates to the field of gene engineering, in particular to a phenolic acid decarboxylase mutant and application thereof. According to the invention, tryptophan at the 30th site of phenolic acid decarboxylase PADCs from Clostridium sp.DSM 8431 is mutated into leucine, and tyrosine at the 136th site of the phenolic acid decarboxylase PADCs is mutated into tryptophan, so that the phenolic acid decarboxylase mutant is obtained. The phenolic acid decarboxylase mutant can catalyze decarboxylation reaction of p-coumaric acid to synthesize p-hydroxystyrene; and hydrocarbonate can also be used as a carboxyl source to catalyze carboxylation reaction of p-hydroxystyrene to synthesize p-coumaric acid. The phenolic acid decarboxylase PADCs has the enzyme activity characteristics of excellent temperature stability and acetonitrile stability, still retains more than 80% of decarboxylase activity after being incubated for 2 hours at 55 DEG C, still retains about 36% of decarboxylase activity after being incubated for 2 hours in acetonitrile with the volume fraction of 50%, can remarkably improve the yield of p-coumaric acid, can reach 13.05 mM when the substrate concentration is 80 mM, and is improved by 74% compared with original phenolic acid decarboxylase PADCs; the method has a wide industrial application prospect and CO2 fixation potential.

The invention relates to the technical field of energetic materials, in particular to a laser response type high-entropy alloy-based initiating explosive material and a preparation method thereof. The initiating explosive material is mainly formed by uniformly loading a heat-sensitive high-energy compound (such as lead stilbeneate or hexa (4-amino-3, 5-dinitropyrazole) copper) on high-entropy alloy nanoparticles containing five or more metal elements, wherein the heat-sensitive high-energy compound is such as lead stilbeneate or hexa (4-amino-3, 5-dinitropyrazole) copper. The preparation method comprises the following steps: mixing acetylacetone metal salt, a surfactant and a reducing agent in an organic solvent, and stirring and reacting at 180-220 DEG C to prepare high-entropy alloy nanoparticles; the nano particles and a high-energy compound are co-dispersed in a mixed solvent containing a dispersing agent, ultrasonic treatment is carried out to form suspension liquid, and freeze drying is carried out to obtain the nano particles. The method is characterized in that the excellent photo-thermal conversion performance of the high-entropy alloy is utilized, under low laser energy irradiation, a high-energy compound can be effectively excited, and stable detonation of secondary charge CL-20 is successfully achieved. According to the invention, the problems of high response threshold and low output energy of the existing laser initiating explosive material are solved.

The invention discloses a method for jointly treating fresh-cut broccoli through edible composite coating and intermittent superheated steam, and belongs to the technical field of agricultural product processing, fresh broccoli is used as a raw material, and the fresh-cut broccoli is obtained through impurity removal, cleaning, cutting, shape finishing, coating, intermittent superheated steam treatment, cooling and packaging. The method is simple in step, short in process period, efficient, energy-saving, green and environment-friendly in operation process, loss of nutrients of the broccoli is reduced through the edible composite coating and intermittent superheated steam treatment, the total color difference of the fresh-cut broccoli is reduced, and the content of total phenols, total anthocyanin, total anthocyanin and total phenolic acid is increased.

The invention provides a combined lactobacillus salivarius YS-UR90 and a preparation method of the combined lactobacillus salivarius YS-UR90. The saliva combined lactobacillus provided by the invention can secrete protease and glycosidase, and the HCT and FLS gene expression levels of the saliva combined lactobacillus are high, so that the saliva combined lactobacillus can promote phenolic acid active ingredients to generate biotransformation during fermentation, and the proportion of chlorogenic acid and the like in fermentation liquor is increased. For example, marine fish oligopeptide and casein phosphopeptides are used as a composite nitrogen source, dandelion powder is used as a functional substrate, and a composite fermentation product obtained through fermentation of saliva and lactobacillus has the effect of promoting uric acid excretion and has a certain protection effect on kidney injury. The compound fermentation product has the effect of improving hyperuricemia mainly by inhibiting XOD activity, increasing serum urea nitrogen level, improving defect expression of uric acid synthesis gene HPRT1, inhibiting expression of uric acid reabsorption URAT1 and GLUT9 genes and improving expression of excretion genes OAT1 and ABCG2.

The invention discloses a redness repairing and relieving composition as well as a preparation method and application thereof. The red repairing and relieving composition comprises a red repairing and relieving functional agent, wherein the components of the red repairing and relieving functional agent comprise a phenolic compound and a phenolic acid compound; the phenolic compound is selected from at least one of hydroxytyrosol and tyrosol; the phenolic acid compound is selected from at least one of caffeic acid, protocatechuic acid, plateau catechuic acid and ethyl protocatechuate. The composition can inhibit release of inflammatory factors, relieve itching, resist inflammation, calm skin and reduce angiogenesis, and meanwhile can enhance the skin water locking capacity, improve the skin water shortage condition and enable the skin to recover to be moisturized, tender and smooth.

The invention belongs to the technical field of natural medicines, and particularly discloses a lipase inhibitor with a specific structure as well as a preparation method and application thereof, the inhibitor is derived from grape leaves and contains C6-C3-C6, 1, 2-benzopyrone, diphenyl ethylene and a phenolic hydroxyl structure, and the lipase inhibitor is a lipase inhibitor with a specific structure. Comprising the following components in percentage by mass: 72.1 to 97.5 percent of flavonoids, 2.5 to 5.1 percent of coumarins, 2.0 to 3.2 percent of stilbenes and 5.1 to 8.3 percent of phenolic acids. According to the lipase inhibitor with the specific structure as well as the preparation method and the application of the lipase inhibitor, the inhibitor can inhibit secretion of lipase, prevent fat from being decomposed into absorbable free fatty acid and monoacylglycerol, and reduce fat absorption; the total cholesterol and the low-density lipoprotein cholesterol are reduced, the blood fat level is improved, and the composition can be applied to preparation of health-care products, functional foods, sports nutritious foods and formula foods for special medical purposes.

The invention discloses application of salvianolic acid A in preparation of a medicine for preventing and treating vulnerable plaques of advanced atherosclerosis. The invention also discloses application of the salvianolic acid A combined with ferulic acid or the salt thereof in preparation of drugs for preventing and treating advanced atherosclerosis vulnerable plaques. Researches find that salvianolic acid A can promote apoptosis of senescent vascular smooth muscle cells and has a Senlytics effect; the salvianolic acid A stabilizes vulnerable atherosclerotic plaques by reducing the load of senescent vascular smooth muscle cells; the salvianolic acid A is in non-covalent binding with ASP54, ALA55, LYS58, ASP93, ASP102 and LYS112 of the HSP90AA1 to play a role in inhibition, and the ASP93 has the greatest contribution to binding free energy. The research of the invention is of great significance to the development and application of drugs with anti-aging effect for stabilizing advanced atherosclerotic vulnerable plaques.

The invention provides a bio-based epoxy emulsifier, a sizing agent, carbon fibers, a composite material and a preparation method of the bio-based epoxy emulsifier. The bio-based epoxy emulsifier is prepared from the following raw material components: polysaccharide, epoxy resin, phenolic acid and a catalyst. The preparation method of the bio-based epoxy emulsifier comprises the following steps: S101, heating epoxy resin to 50-100 DEG C until the epoxy resin is completely molten, then adding polysaccharide, and reacting at 80-110 DEG C for 4-15 hours to obtain polysaccharide modified epoxy resin; s102, the polysaccharide modified epoxy resin is heated to 120-200 DEG C, phenolic acid and a catalyst are added, a reaction is conducted for 5-18 h, and the bio-based epoxy emulsifier is obtained. No organic solvent is needed in the process of preparing the sizing agent by using the bio-based epoxy emulsifier provided by the invention, and meanwhile, the sizing agent prepared by using the emulsifier can improve the performance of carbon fibers and composite materials.

The invention relates to application of salvianolic acid B in preparation of anti-inflammatory and anti-radiation drugs. Experimental verification shows that within a safe concentration range, salvianolic acid B can effectively inhibit the release amount of NO in RAW264.7 cells and the expression level of proinflammatory factors TNF-alpha, IL-6, IL-1beta and IFN-gamma in the RAW264.7 cells under the co-stimulation of LPS and radiation, and meanwhile, the phagocytic ability of the cells is improved; the salvianolic acid B remarkably inhibits the H2O2 expression level of RLE-6TN cells and relieves the early apoptosis of the RLE-6TN cells, and the results show that the salvianolic acid B can be used for preparing the anti-inflammatory and anti-radiation drugs. Compared with a traditional clinical medicine, the salvianolic acid B has the advantages of low toxicity and low side effect as a natural traditional Chinese medicine product.

The invention relates to a method for efficiently degrading phenolic acid autotoxic substances in continuous cropping of crops by using biochar immobilized laccase. Pyrolyzing the tobacco stem and straw powder in a muffle furnace to obtain tobacco stem and straw biochar BC; the preparation method comprises the following steps: soaking BC and potassium carbonate in a mass ratio of 1: 4 for 2-4 hours, drying, pyrolyzing in a muffle furnace after drying, air-cooling to room temperature, washing with deionized water, drying and sieving to obtain potassium carbonate modified tobacco stem and straw biochar CBC; dispersing CBC in an HNO3 solution, mixing for 3-5 hours at the speed of 300rpm and the temperature of 30 DEG C, washing with deionized water until the pH value is 7, drying overnight, and storing at room temperature to obtain functionalized CBC; placing the functionalized CBC in a 50mM phosphate buffer solution for 20-30 hours, adding 1.0% w / v glutaraldehyde with the same volume as the functionalized CBC, oscillating at room temperature, washing with redundant glutaraldehyde deionized water, and drying overnight to obtain glutaraldehyde-containing CBC; and carrying out enzyme immobilization on the glutaraldehyde-containing CBC and a 5-10U / mL laccase solution according to a solid-to-liquid ratio of 1g: 10mL at 25-30 DEG C to obtain the immobilized laccase. Autotoxic substances are effectively degraded, the yield and quality of peppers are improved, and ecological restoration of soil is promoted.

The invention belongs to the technical field of biological fertilizers, and discloses a phenolic acid degrading soil conditioner and application thereof, and the phenolic acid degrading soil conditioner is prepared by using robinia pseudoacacia roots as a raw material. The active ingredient of the soil conditioner is robinia pseudoacacia root exudates, and the preparation method of the soil conditioner comprises the following steps: performing water culture on robinia pseudoacacia plants in a container for 3-5 days to obtain a collection solution containing the robinia pseudoacacia root exudates. Another preparation method of the soil conditioner comprises the following steps: S1, mixing robinia pseudoacacia roots and water in a mass ratio of 1: (1-2), and standing for 3-5 days under an illumination condition to obtain a mixed solution; s2, homogenizing the mixed solution, adding cellulose degrading bacteria, and fermenting for 5-7 days to obtain a root fermentation product; and S3, mixing the root fermentation product with a carrier to prepare the phenolic acid degradation fertilizer. The soil conditioner is simple in preparation method, low in cost and safe and environment-friendly to use, the content of phenolic acid substances in continuous cropping soil of crops can be effectively reduced, harm of the phenolic acid substances to growth and development of konjak is relieved, and konjak planting is facilitated.

Preparation of cannabinoid formulations containing: Δ9-tetrahydrocannabinol (Δ9-THC), Δ8-tetrahydrocannabinol (Δ8-THC), Δ9-tetrahydrocannabinolic acid (THCa), cannabidiol (CBD), cannabidiolic acid (CBDa), cannabigero (CBG), cannabichromene (CBC) and cannabinol (CBN), either alone or in combinations henceforth known as cannabis, have been created using an emulsification process to encapsulate cannabinoids. The aqueous-based method involves micellular encapsulation of cannabinoids, a method that has been used to increase the bioavailability of poorly permeable, lipophilic drugs. The present invention demonstrates the viability of transdermal delivery with gels and patches for consistent and sustained cannabinoid dosing.

The invention discloses a nanometer emulsion based on a citrus extract and a preparation method of the nanometer emulsion. The nano-emulsion takes citrus pectin as a main chain, gallic acid, ascorbic acid-phosphate and oleic acid are sequentially grafted, the oleic acid is lipophilic, the rest is hydrophilic, and the amphiphilic pectin can form the whitening nano-emulsion which can be stably stored for a long time. The natural citrus extract is used for replacing traditional synthetic components, has the anti-oxidation, whitening and anti-inflammatory effects, can reduce the skin sensitivity risk, and conforms to the green beauty makeup trend; meanwhile, the oil phase is anchored by a hydrophobic block, steric hindrance is formed by a hydrophilic block, the stability of the emulsion is remarkably improved, and the shelf life is prolonged to 24 months; phenolic acid and ascorbic acid contained in the traditional Chinese medicine composition can effectively scavenge free radicals, inhibit tyrosinase activity, reduce melanogenesis and reduce release of a proinflammatory factor IL-6; the composition is low in cost, environment-friendly, suitable for the fields of cosmetics and medicines and wide in market prospect.