1205 results about "Phenolic acid" patented technology

The present invention relates to extraction process of total tanshin phenolic acid, the preparation process and application of its preparation. The total tanshin phenolic acid and its preparation mayuse in preparing medicine for preventing and treating cardiac and cerebral vascular diseases, senile dementia and hypomnesia caused by cerebral ischemia. The total tanshin phonelic acid prepared based on the process of the present invention has rich active components of tanshin and obvious pharmacological effect, stable performance and les stoxicity and is safety and reliable.

This invention concerns the method of extracting salvianolic acid A from Chinese crude drug: danshen root, and the quality control methods and drug combinations, and the application of this drug. It can be used in the preparation of the prevention drugs for cardiovascular disease, liver damage, liver fibrosis, pulmonary fibrosis and other.

A process for preparing danshinolic acid A from red sage root includes such steps as extracting in water or alcohol, concentrating, high-temp and -pressure reaction, purifying by chromatographic column, regulating pH value, extracting in organic solvent, concentrating and drying.

The present invention provides a technique method which separates lignose, hemicellulose, cellulose and phenolic acid from lignocellulosic biomass. Under the selected pH value and temperature, the lignocellulosic biomass is processed by hot water, so that the hemicellulose and the phenolic acid are preferentially resolved in aqueous solution, delignification processing is carried out, organic solvent or alkaline solution is applied to carry out high-temperature cooking, the generated liquid-solid phase product can be easily and effectively separated, and thus the hemicellulose, the cellulose and the phenolic acid can be respectively obtained. Moreover, enzymatic treatment can be selectively added, and a solid phase product is obtained at the first stage of a group of enzymatic treatment with a cooperative effect. Because the ferulic acid linkage between the hemicellulose and the lignose can be selectively broken, the separation of the chemical components has a high yield rate and high purity.

An active component of red sage root, its slow-releasing agent, its medicinal application, and its preparing process are disclosed. In its effective region, the content of general solviolic acid is more than 80% and the content of salviolic acid A is more than 10%. Said slow-releasing agent is prepared through extracting in water or alcohol ic water, getting a supernatant, removing alcohol, dissolving in water, then washing to remove inpurities, eduting with alcohol, recovering alcohol eluent and drying. It can be used to prepare the medicine to prevent and cure cardiovascular and cerebrovascular diseases.

The invention is concerned with a kind of extract of total ketone of salviae miltiorrhizae and total phenolic acid and its produce method form radix Salviae Miltiorrhizae. The extract of total ketone of salviae miltiorrhizae has cryptotanshinone, tanshinone I, tanshinone IIA, methyl Tanshinon, dihydrotanshinon I and ramification. The extract of total phenolic acid has salvianolic acid A, salvianolic acid B, protocatechuic aldehyde and ramification. The extract can be got form one or arbitrary compound of extraction with solvent method, macroporous resin method, column chromatography and liquid-liquid counter-current chromatography. The summation of the content to each total ketone of salviae miltiorrhizae is 20 to 100 percnte (w / w) of the extract of total ketone of salviae miltiorrhizae, the contene of cryptotanshinone, tanshinone I and tanshinone IIA is 5 to 100 percent (w / w) of whole content of total ketone of salviae miltiorrhizae. The summation of the content to each total phenolic acid is 5 to 100 percent (w / w) of the extract of the radix salviae miltiorrhizae total phenolic acid. The content of salvianolic acid B is the 5 to 100 percent (w / w) of the whole salvianolic acid.

The invention discloses a method for controlling the quality of ginkgo leaves and an extract thereof. After the screening of a great quantity of experiments, the method adopts ultra-high performance liquid chromatography for detection; and the method can be used for detecting three types of components including flavone, terpene lactones and phenolic acid, twenty-four active compounds in total, in the ginkgo leaves and the extract of the ginkgo leaves. The results of the experiments prove that the method is high in detection sensitivity and good in stability, and can be used for objectively, comprehensively and accurately evaluating the quality of ginkgo leaf medicines, the extract of the ginkgo leaves and ginkgo leaf preparations, thus having important significance on controlling the quality and guaranteeing the clinical treatment effect.

A purified cannabidiol (CBD) extract and / or cannabidiolic acid (CBDA) extract is isolated from industrial hemp and comprises less than 0.5 wt % organic impurities as measured by HPLC and 1H NMR spectroscopy exhibits no detectable peak at 4.07 ppm as measured by 1H NMR spectroscopy. The CBD and / or CBDA extract is in crystalline form. The CBD extract exhibits a melting point as measured by differential scanning calorimetry (DSC) of 69-70° C. Dry powder compositions comprise such extracts. Additional dry powder compositions comprise polyvinylpyrrolidone and an amorphous CBD extract. An adduct comprises CBD and / or CBDA bonded to a paramagnetic trivalent lanthanide (III) metal chelate.

The present invention relates to processes for the treatment of liquors derived from oil-bearing fruit, for example from oil palm fruit, and to products therefrom. Typically a process involves removal of undissolved solids, oleaginous parts, colloids and higher weight molecules from the vegetation liquor to give an aqueous fraction containing phytochemicals, for example, flavonoids, phenolic acids and hydroxy acids. Subsequently, pH adjustment and solvent extraction upon said aqueous fraction realise an extract rich in hydroxy acids or phenolic acids or flavonoids or any combination thereof Applications of the substances subject of this invention are to be found in drinks, edible products, tonics, health supplements, antioxidant additives, cosmetics, soaps, shampoos, detergents, drugs or medicinal products.

A process for extracting danshinolic acids A and B from red sage root includes soaking in hot water, collecting liquid extract, centrifugal separating, ultrafiltering of supernatant, extracting by reverse-phase column or acetate, acidifying, separating organic phase to recover solvent, and separating by reverse-phase column. A freeze dried injection for preventing and treating liver injury and hepafibrosis is prepared from said danshinolic acids A and B and additives. Its advantages are high purity and high output rate.

The invention discloses a salvianolic acid A for injection and its preparing process, wherein high efficiency liquid chromatography method is employed to determine salvianolic acid A in the raw material medicaments, the content of salvianolic acid A is between 92% and 100%, two foreign substances are present. The mass ratio of salvianolic acid A and anti-oxidizing agent in the preparation is 2-50:1, the pH of the injection is controlled to 3-7.

The present invention provides an apparatus for simultaneously producing different cannabinoids in different ratios. Specifically, the apparatus according to the invention produces tetrahydrocannabinolic acid (THCA) and cannabichormenic acid (CBCA) or cannabidiolic acid (CBDA) in different ratios, and it comprises a bioreactor that comprises a first automated supply system configured to deliver a substrate and a cannabinoid acid synthase, and a second automated system to cease the reaction; an extractor configured to recover the cannabionoids so produced; and a controller configured to modify at least on property of the reaction.

Described herein are methods to enhance the production of more highly fermentable carbohydrates in plants, especially forage grasses. The invention provides for transgenic plants transformed with expression vectors containing a DNA sequence encoding ferulic acid esterase I from Aspergillus, preferably A. niger. The expression vectors may optionally comprise a DNA sequence encoding xylanase from Trichoderma, preferably T. reesei. Expression of the enzyme(s) is targeted to specific cellular compartments, in specific tissues and under specific environmental conditions. Uses of this invention include, but are not limited to, forage with improved digestibility for livestock, and enhanced biomass conversion.

The present invention relates to a compound salvia active component group and its slow-release preparation, medicinal application and preparation method. It features convenient administration and good stability. Said invention contains salvia active component (salvia total phenolic acid content is above 50%), notoginseng active component (notoginseng total saponin content is above 50%) and natural (or artifiical) borneol and its cyclodextrin inclusion. It can be made into various slow-release preparations, and is obtained by using water or pure water to make extraction, dissolving in water, removing impurity by using water to wash and recovering and drying. It can be used for making medicine for curing angiocardiopathy and cerebrovascular diseases.

The invention discloses a natural food additive which belongs to the technical field of food additives. The natural food additive is characterized by comprising the following substances in percentage by weight: 20%-30% of a red bayberry anthocyanin extractive, 10%-20% of a purple sweet potato anthocyanin extractive, 10%-40% of a mulberry anthocyanin extractive, 10%-50% of an okra pectin and polysaccharide extractive, 10%-20% of a folium ginkgo extractive, 0.0005%-0.0008% of selenium yeast, 2%-10% of seaweed meal, and 5%-10% of ganoderma lucidum spore powder. The natural food additive has strong functions of oxidation resistance, aging resistance, fatigue resistance, invigorating the kidney, strengthening Yang, improving immunity and reducing blood sugar, cholesterol and triglyceride. After the natural food additive is detected, the total anthocyanin content is 15%-36%, the total flavone content is 16%-28% and the total phenolic acid content is 18%-29%.

The invention discloses a water soluble phenolic acid extract which is obtained by extracting from a medical plant of a clerodendranthus spicatus by the chemical method. The pharmacological experiment testifies that the extract has strong physiological activity and especially has strong physiological activity of resisting the generation of uric acid, and the extract is used for remedying the pain-louse and has good effect of the treatment towards the urinary tract infection or the urinary tract concretion and has little toxic and side effect.

The present invention discloses one salvianolic acid A preparing process. The salvianolic acid A preparing process includes extract red sage material with water or alcohol, concentrating the extracted liquid, purifying salvianolic acid A in chromatographic column, extracting with organic solvent in regulated pH value, concentrating and drying to obtain salvianolic acid A. The said process has high salvianolic acid A extracting rate, and high salvianolic acid A product purity, up to 90 %, stable quality, and is suitable for large scale production.

The object of the present invention is to provide a photobase generator capable of efficiently generating amines (tertiary amines and amidine) high in catalytic activity by sensing light with a wavelength of from 350 to 500 nm (especially, from 400 to 500 nm).The present invention is a photobase generator characterized in being represented by general formula (1) or (2).Y+ is a quaternary ammonio group of general formula (3) to (5), and X− is a counter anion selected from among a borate anion, a phenolate anion, and a carboxylate anion.

The invention discloses a method for manufacturing salvianolic acid A of danshen root, which is characterized in that total salvianolic acid in the danshen root is transformed into salvianolic acid A by adopting a certain method. The method increases greatly the extraction yield of salvianolic acid A. The salvianolic acid A is prepared into tablets, capsule, granula, soft capsule, micro pill, dripping pill and oral liquid, which have good pharmacological action indicated by pharmacological experiments.

There is provided a surfactant composition, a stable, self emulsifiable water borne curing agent composition, and methods for the manufacture of each, and two component water borne curable epoxy resin compositions. The surfactant composition comprises the reaction product of an a) a phenolic acid substituted with at least one carboxyl group and at least one hydrocarbyl group having at least 1 carbon atom; b) a polyepoxide compound; c) a polyamine compound having at least two primary amine groups; d) a reactive surfactant; and optionally e) a monoglycidyl, monocarboxylic acid, or monoisocyanate capping agent; wherein the reactive surfactant comprises an epoxide, a carboxylic acid or anhydrides thereof, or an isocyanate functional moeity, and a hydrophilic moiety comprising a polyoxyalkylene monool or polyol residue. The reactive surfactant comprises a compound represented by one of the following formulas:

The preparation method of chlorogenic acid and isochlorogenic acid compound uses leaf of lonicera macranthoides as raw material and adopts the following steps: defatting, using ethyl alcohol with a certain concentration to make extraction, concentrating ethyl alcohol extract, feeding the concentrated ethyl alcohol extract into macroporous resin column, eluting by using 0-10% ethyl alcohol to obtain chlorogenic acid and its isomer, then eluting with 20-35% ethyl alcohol to obtain isochlorogenic acid and its isomer; mixing then according to different ratio and making treatment so as to obtain total phenelic acid, its content can be up to 80%.

The invention relates to a DanShen fingerprint spectrum quality control method, comprising that (1), adding 1.0g DanShen powder into carbinol to extract via microwave, (2), washing flow phase gradient that the Alltima C18 column is 4.6mm, 250mm, and 5mum, checking wavelength is 280nm, flow speed is 1.0ml / min, the column temperature is 20-40Deg. C, and the sample amount is 10-20ul, (3), building standard fingerprint spectrum that the first peak is DanShen element, the eighth peak is rosmarinic acid, the ninth peak is alkannic acid, the tenth peak is phenolic acid B, the eleventh peak is phenolic acid B isomer, the fourteenth peak is cryptotanshinone and the fourteenth peak is tanshinone IIA, (4), controlling the quality of fingerprint spectrum that the check peak relative holding times are 0.21 of DanShen element, 0.91 of rosmarinic acid, 0.93 of alkannic acid, 1.00 of phenolic acid B, 1.04 of phenolic acid B isomer, 0.92 of cryptotanshinone and 1.00 of tanshinone IIA, (5), the DanShen planting collecting method. The invention can control the quality of materials to assure the stable quality of product.

The invention relates to a method for furthering separating and purifying salvianolic acid from Danshen extract fluid, which comprises that prepares, breaks and extracts Danshen via water solution, acidifies extract to adjust pH and adds salt to process post-treatment, processes dynamic continuous adsorption and elution on the treated extract at chromatography column stuffed with resin adsorbent, elutes via water, collects and concentrates eluent, elutes via gradient ethanol solution, segmented collects and concentrates eluent, and dries the concentrates solution to obtain product. The invention can simply, effectively and quickly separate and purify various salvianolic acids, wherein test on different salvianolic acid products shows that the highest yields of tanshinol, alkannic acid, rosmarinic acid, salvianolic acid A and salvianolic acid B are 35. 55%, 70.65%, 99.27%, 82.78%, and 89.34%, and relative highest purities are 95.32%, 65.05%, 29.40%, 33.93%, and 82.35%, which are near or higher than the result of purification on the goal of single component.

A method for the extraction and isolation of the terpene and isoprenoid compounds from plant material, followed by a centrifugal force induced selective crystallization of isoprenoids resulting in a separation of terpene and isoprenoid fractions. This this method is suitable for the extraction of cannabinoids from Cannabis and the enrichment tetrahydrocannabinolic acid and reduction of tetrahydrocannabinol in an extract.

The comprehensive gingko outer seed coat utilizing process belongs to the field of gingko processing technology. Of the fresh gingko outer seed coat as material, the components capable of being fermented are water extracted as the fermentation culture medium and is fermented and extracted to produce alcohol, and the residue after water extraction is leached with 80 % concentration alcohol solution as the leaching solvent. The leached liquid is vacuum concentrated, separated and purified with macroporous resin LSA-21 and silica gel column successively to obtain gingko phenonic acid and similar matter in purity over 90 %. The present invention is significant in utilizing gingko resource and protecting ecologic environment.

The invention relates to a method for ultrasonically extracting and preparing flavonoids pigment from chrysanthemum, belonging to the field of food processing and especially used for deep processing chrysanthemum. The process comprises the following steps of: blanching, drying and hermetically storing chrysanthemum at a low temperature, filtering by using a 100-mesh sieve, soaking in 60% ethanol as an extraction solvent in a liquid-material ratio of 40 / 1 (v / w) for 2 h, ultrasonically extracting a dry base in a water bath at 70 DEG C for 10 min to acquire the yield of 13.34 percent; and evaporating in the water bath at 70 DEG C by using the 60% of ethanol extraction liquid in a rotating way to release an ethanol taste; and freezing and drying in vacuum, wherein analytical compositions reach 52.01% of the dry base and comprise flavonoids, linarin, flavonoids (luteolin, acacetin and apigenin), flavonols (quercetin), phenolic acid (chlorogenic acid and caffeic acid), metal elements, and the like. The invention provides a new method for deep processing the chrysanthemum and has strong maneuverability, good safety and good repetitiveness.

The invention provides a method for simultaneously detecting main components of paeoniflorin, beta ecdysterone, laetrile, mulberroside A, caffeic acid, ferulic acid, salvianolic acid B, astragaloside, formononetin, cryptotanshinone and tanshinone IIA of a Naoxintong capsule in a plasma sample by a liquid chromatography-tandem mass spectrometry (HPLC-MS / MS). In a liquid chromatogram, a mobile phase consists of acetonitrile and a formic acid aqueous solution of which the volume fraction is 0.1%, and gradient elution is used. A mass spectrum uses a quick positive and negative ions switching and analyzing mode and an MRM (Multiple Reaction Monitoring) scanning manner. After the Naoxintong capsule is taken, the situations of the changes of the blood-medicine concentration of several kinds of main components in the plasma of a rat are detected at the same time. The methodological survey results indicate that the established method conforms to determination requirements on biological samples in a body; the method is good in sensitivity, high in specificity, stable, reliable, and suitable for detecting substances with lower contents.

The invention discloses a golden fungus gingko yellow wine. Alcoholic strength is 8-45%, general flavone content is more than or equal to 1-5 mg / L, ginkgolide is more than or equal to 1-6 mg / kg, total triterpenoid compound content is 30-800 mg / L, protein is more than or equal to 60 mg / kg, soluble sugar is less than or equal to 2.0%, gingko phenolic acid is less than or equal to 5 ppm, and hydrocyanic acid is less than or equal to 0.1 mu g / L. A double-enzyme method is adopted to hydrolyze gingko to obtain gingko hydrolysate which contains active compounds, such as ginkgo flavone, triterpenoid compound, amino acid, peptide, starch and the like, wherein the content of amino acid and peptide is more than or equal to 1%, and starch content is more than or equal to 2%. After being added with gingko leaf extract, the hydrolysate is converted by golden fungus strains, and rice koji is added to obtain fermentation liquor; the fermentation liquor is filtered and blended to obtain golden fungus gingko yellow wine. The wine contains gingko and golden fungus active ingredients and has the efficacy of preventing diabetes and hyperlipidemia, preventing oxidation and improving immunity of body.

The invention relates to a method for increasing the contents of tanshinone and salvianolic acid in a salvia miltiorrhiza hairy root by using a transgene AtMYC2, belonging to the technical field of gene engineering. The method comprises the steps of constructing a high-efficiency expression vector of a plant by using an arabidopsis transcription factor AtMYC2, and carrying out genetic transformation on salvia miltiorrhiza leaves to obtain a gene AtMYC2 overexpressed transgenetic salvia miltiorrhiza hairy root; analyzing the expression of AtMYC2 in the transgenetic salvia miltiorrhiza hairy root and related genes in biosynthetic pathways of tanshinone and salvianolic acid through qRT-PCR; measuring the contents of tanshinone and salvianolic acid in the transgenetic salvia miltiorrhiza hairy root by using a high-performance liquid chromatography (HPLC); and measuring the antioxidant activity of tanshinone and salvianolic acid in the transgenetic salvia miltiorrhiza hairy root by using a DPPH free radical scavenging method. The invention provides the method for simultaneously increasing the contents of tanshinone and salvianolic acid in salvia miltiorrhiza hairy root and also provides a novel high-quality raw material for producing tanshinone and salvianolic acid with important clinic demands so as to have the positive promoting significance and application value for relieving the problem that the drug resources of tanshinone and salvianolic acid are short.