240 results about "Spermidine" patented technology

The present invention is a method for the biosynthesis of hundreds of compounds, mainly found in the cannabis plant. The starting material for these compounds can be any biological compound that is used / produced in a biological organism from the sugar family starting materials or other low cost raw materials processed via enzymes or within organisms to give final products. These final products include, but are not limited to: cannabinoids, terpenoids, stilbenoids, flavonoids, phenolic amides, lignanamides, spermidine alkaloids, and phenylpropanoids. Specifically, the present invention relates to the regular / modified / synthetic gene(s) of select enzymes are processed and inserted into an expression system (vector, cosmid, BAC, YAC, phage, etc.) to produce modified hosts. The modified host is then optimized for efficient production and yield via manipulation, silencing, and amplifying inserted or other genes in the host, leading to an efficient system for product.

The invention belongs to the fields of molecular biotechnology and experimental methods and specifically relates to a fluorescence quantitative PCR reaction solution. The solution is characterized by comprising a PCR reaction reinforcing agent which is one or more selected from the group consisting of dimethyl sulfoxide, glycerin, bovine serum albumin, methanamide, polyethylene glycol 6000, spermidine, ammonium sulfate and betaine, e.g., dimethyl sulphoxide, bovine serum albumin and betaine. The fluorescence quantitative PCR reaction solution further comprises heatproof DNA polymerase having 5'-3' polymerase activity and 5'-3' exonuclease activity and preferably comprises heatproof DNA polymerase and hot-start heatproof DNA polymerase, both of which having 3'-5' exonuclease activity. The invention also relates to a kit containing the reaction solution and a method of applying the reaction solution in fluorescence quantitative PCR.

Intravitreal administration of a JNK-inhibitory peptide less than 150 amino acids in length, containing at least one D-amino acid, and having (a) a JNK-inhibitory sequence of at least any of SEQ ID NO: 1 and SEQ ID NO: 2, and (b) a transport sequence of at least any of SEQ ID NO: 3 and SEQ ID NO: 4 suppressed spermidine-induced retinal pigment epithelial damage, tunicamycin-induced photoreceptor cell damage, and laser-induced choroidal neovascularization. Thus, the JNK-inhibitory peptide of the present invention is useful for prophylaxis or therapy of a retinal disease. By the use of this JNK-inhibitory peptide, a drug and a method are provided which are capable of preventing or treating a retinal disease even by topical administration to the eye, and use of the JKN-inhibitory peptide for manufacturing the drug is also provided.

The invention belongs to the technical field of plant growth regulators, and relates to a wheat cold resistance regulating agent. The wheat cold resistance regulating agent comprises the specific components of 100-1000 mg / L of enteromorpha polysaccharide, 0 mg / L or 100-200 mg / L of ortho-hydroxybenzoic acid, 0 mg / L or 0.01-0.1 mg / L of brassinolide, 0 mg / L or 100-1000 mg / L of glycine betaine, 0 mg / L or 10-100 mg / L of spermidine, 0 mg / L or 1000-20000 mg / L of surfactant and water as a solvent, wherein the enteromorpha polysaccharide is low-molecule weight enteromorpha polysaccharide with a molecule weight of 1 k-50 kDa; and the related surfactant is one or a mixture of Tween series, Span series, OP series and / or NP series. A use method of the wheat cold resistance regulating agent comprises the step of spraying / irrigating a wheat seedling, or planting after soaking a wheat seed for 6-12 h. The wheat cold resistance regulating agent is simple in components, easily available in raw materials, low in cost, remarkable in effect and environmentally friendly.

The invention discloses a cold-resistant agent for growth of Chinese trichosanthes. The cold-resistant agent comprises the following components by weight: 5-7.5mg of abscisic acid, 0.03-0.06g of salicylic acid, 0.6-1g of calcium nitrate, 0.035-0.04g of putrescine, 0.05g of spermidine, 0.45g of urea, 0.3g of monopotassium phosphate, 0.04g of citric acid, 0.1g of malic acid, 0.05g of chitosan, and 1L of water. The invention simultaneously discloses a using method of the cold-resistant agent for growth of Chinese trichosanthes. The method comprises the following steps of: spraying leaves of a Chinese trichosanthes plant by a Chinese trichosanthes cold-resistant agent until the leaves drip 12-48 hours before a cold wave comes. By adopting the cold-resistant agent for growth of the Chinese trichosanthes disclosed by the invention, the ability of the Chinese trichosanthes for resisting low-temperature damage can be obviously enhanced; and the stability of a Chinese trichosanthes cell membrane also can be enhanced.

The invention discloses a lysis solution and an application thereof in tissue or cell preservation and RNA (Ribonucleic Acid) extraction. The lysis solution comprises a lysis solution A and a lysis solution B, wherein the lysis solution A comprises 2-4mol / L guanidinium isothiocyanate, 5-10mmol / L sodium chloride, 10-15mmol / L potassium chloride, the lysis solution B comprises 2-4mol / L guanidinium isothiocyanate, 1-5mmol / L spermidine, 30-40vt% of water saturated phenol, 0.4-0.5mol / L ammonium thiocyanate, 7-8vt% of glycerinum, 0.1-0.5mol / L sodium acetate and 0.2-0.5wt% of SDS (Sodium Dodecyl Sulfonate). When RNA is extracted by using the lysis solution A and the lysis solution B, tissue or cells do not need to be washed, and thus the extraction efficiency is remarkably improved; and due to synergistic effects of the components, the extracted RNA is high in purity and high in yield, and the purpose that animal RNA is extracted in laboratories rapidly with low cost and high quality in a large scale can be realized.

The invention relates to a detection kit for ureaplasma urealyticum (UU) nucleic acid by utilizing RNA constant-temperature amplification. The detection kit comprises a urine sample preserving solution, a nucleic acid extraction solution, a UU reaction solution, a UU detection solution, an SAT (Spermidine / Spermine N1-Acetyltransferase) enzyme solution, a UU positive control and a UU negative control. The detection kit provided by the invention has the analytical sensitivity of 50 CFU / reaction for detecting UU, and has the characteristics of high specificity and high sensitivity; and an amplification product RNA is easy to degrade in a natural environment and causes low pollution.

The invention provides a method for extracting total RNA from mature grape fruits. The method for extracting the total RNA from the mature grape fruits particularly comprises the steps of extraction by adopting CTAB and chloroform and acidic water phenol purification. According to the method for extracting the total RNA from the mature grape fruits, polyvinylpyrrolidone, spermidine, beta-mercaptoethanol and high-concentration sodium chloride are added into an extracting solution of a traditional CTAB method, the activity of the RNA enzyme can be efficiently restrained, and the interference of polysaccharide and polyphenol substances with RNA can be effectively prevented. In the extraction process, the acidic water phenol purification is utilized for crude extraction of the RNA, DNA which is coprecipitated with the RNA can be removed to the maximum extent, and residual protein and polysaccharides can be cleaned away. The method for extracting the total RNA from the mature grape fruits is low in cost, good in stability, high in purity of extracted RNA samples and good in integrality.

A method for diagnosing heart failure in a subject is provided. The method includes steps of measuring a biological sample of the subject to obtain an amount of at least one biomarker selected from the group consisting of xanthine, spermidine, propionylcarnitine, butyrylcarnitine and P-cresyl sulfate; and comparing the amount of the at least one biomarker to a reference. Moreover, the present invention relates to a method for staging heart failure or evaluating a prognosis of heart failure in a subject.

Water-based drilling mud lubricants using a blend of fatty acid polyamine salts and fatty acid esters give synergistically better lubricity results than either component used separately. For example, the blends with different ratios of fatty acid diethylenetriamine salt and fatty acid methyl ester demonstrate much better lubricity in water-based drilling fluids than those where only fatty acid diethylene-triamine salt or fatty acid methyl ester are separately used. The amines in fatty acid amine salt might also include other polyamines, such as butanediamine pentamethylenediamine, spermidine, spermine, propylene diamine and propylene polyamines. The fatty acid esters might also include fatty acid ethyl ester, fatty acid glycerol ester and fatty acid trimethylolpropane ester. The carbon numbers of the fatty acids used to make the components in the lubricant blend may range from C4 to C28.

The invention belongs to the technical field of plant growth conditioning agents, and relates to a plant salt resisting conditioning agent. The plant salt resisting conditioning agent is prepared from porphyra polysaccharides with the concentration of 100-1,000 mg / L, salicylic acid with the concentration of 0 mg / L or 100-200 mg / L, brassinolide with the concentration of 0 mg / L or 0.01-0.1 mg / L, glycine betaine with the concentration of 0 mg / L or 100-1,000 mg / L, spermidine with the concentration of 0 mg / L or 10-100 mg / L, a surfactant with the concentration of 0 mg / L or 1,000-20,000 mg / L and water, wherein the water serves as a solvent, and low-molecular-weight porphyra polysaccharides with the molecular weight of 1 k-50 k Da are adopted as the porphyra polysaccharides. Accordingly, novel application of the porphyra polysaccharides is supplied, and the porphyra polysaccharides can be used for preparing the plant salt resisting conditioning agent; meanwhile, by compounding the porphyra polysaccharides with the biological source induced resisting substances such as salicylic acid, brassinolide, glycine betaine and spermidine to prepare the salt resisting conditioning agent, the comprehensive resistance of plants on salt stress can be improved, and normal growth and development of the plants are guaranteed; according to the salt resisting conditioning agent, the components are simple, the raw materials are easy to obtain, the cost is low, the effect is significant, and environmental friendliness is achieved.

The invention relates to a culture solution capable of improving the content of resveratrol in polygonum cuspidatum callus and a culture method. The culture solution is composed of an MS basic culture medium, 6-benaylaminopurine, 6-glycosylaminopurine, naphthylacetic acid, spermine, spermidine and sucrose, wherein the concentration of the 6-benaylaminopurine is 0.5mg / L-1.8mg / L, the concentration of the 6-glycosylaminopurine is 0.3mg / L-1.0mg / L, the concentration of the naphthylacetic acid is 0.15mg / L-0.45mg / L, the concentration of the spermine is 1.0mg / L-4.5mg / L, the concentration of the spermidine is 0.5mg / L-1.25mg / L and the concentration of the sucrose is 20g / L-50g / L; and the pH value of the culture solution is 5-6. When the polygonum cuspidatum callus is cultured by using the culture solution provided by the invention, the content of the resveratrol can reach 0.12%-0.18% of the dry cell weight; and the culture solution has the beneficial effect of improving the content of the resveratrol in the polygonum cuspidatum callus.

The invention discloses a compound spraying agent and application thereof in promotion of crop kernel filling and seed viability. The compound spraying agent is prepared from a component A and a component B according to the volume ratio of 1:(1-100), wherein the component A takes water as a solvent and contains 1-50 mM of spermidine, 1-15 mM of jasmonic acid methyl ester and 10-50 mM of ethanol, and the component B is a sweet corn young ear extracting solution. According to the compound spraying agent, spermidine and jasmonic acid methyl ester are taken as effective ingredients of the spraying agent and mixed with the sweet corn young ear extracting solution, and by spraying the spraying agent to leaf surfaces or female ears of crops at the appropriate concentration, dry matter accumulation of sweet corn kernels is effectively promoted, the thousand kernel weight of sweet corn is increased, and the harvested seed viability is improved.

The invention discloses a high spermidine conjugate which is described by follow general formula: R' is H, halogen atom, alkyl, alkoxy or amido. The inventive high spermidine conjugate is a new composition which is used for anti-tumor medicine and has a good target-oriented drug delivery capacity. The inventive material is cheap. The reaction condition is gentle and the yield is high.

The present invention provides methods for improving the specificity of nucleic acid amplification comprising incubating a nucleic acid molecule with a polymerase-Sso7 DNA binding domain conjugate and arginine, spermidine, or spermine. The present invention also provides reaction mixtures and kits for improving the specificity of nucleic acid amplification.

The invention discloses an efficient liquid-phase chromatographic detection method for content of polyamines (putrescine, spermidine and spermine) in a goose tissue. Chromatographic conditions of the method are as follows: a C18 chromatographic column, 5 mu m, 4.6*250mm; a volume ratio of mobile phase methanol: water of 62:38; flow velocity of 1.0 mL / minute; ultraviolet detection wavelength of 229 nm; and a column temperature of 25 DEG C. A tissue solid-phase extracting method comprises the following steps: adding 0.1g of tissue into 1 mL of 5% HClO4 and 10 mu L of an internal standard substance for homogenizing and extracting supernatant; adding 2 mL of 2.5 mol / mL NaOH and 1.4 mu L of benzoyl chloride, carrying out water-bath for 30 minutes at a temperature of 40 DEG C; adjusting pH value to 7.0, then, adding into the C18 column, and flushing by 15 mL of water and 15 mL of 15% methanol; and eluting by 0.5 mu L of methanol. The scheme can effectively separate putrescine, spermidine and spermine in the tissue, and can quickly and accurately detect content of the polyamines in the goose tissue, is simple and convenient to operate, high in accuracy and good in repeatability.

The invention relates to a synthesizing method of spermidine in the field of medical intermediates. The method is characterized by comprising the following steps: firstly, preparing Cbz (carbobenzoxy)-monoprotected 1,4-butanediamine; performing addition reaction with vinyl cyanide; and finally, reducing the cyano group in the addition product, and removing Cbz protection to obtain a high-purity spermidine product. The method has a reasonable design and the advantages of easily available raw materials, simpleness in operation, high product purity, easiness in industrialization and the like, solves the problem of high commercial price of conventional spermidine, is advantageous to improving the position of China in the field of international medical intermediates, and can produce positive effect in the aspect of novel medicament development.

The invention particularly discloses an insect attractant capable of improving the pollination rate and the fruit set percentage of a jujube tree, and application of the insect attractant. The insectattractant comprises a nutrient solution and an animal and vegetable decomposition liquid, wherein the nutrient solution comprises the following ingredients by weight percent: 27.56-38.38% of honey, 0.01-0.09% of putrescine, 0.01-0.09% of spermidine, 0.1-1.3% of salicylic acid, 0.1-0.5% of gibberellin and 60.36-71.5% of urea; the animal and vegetable decomposition liquid is a supernate obtained byfiltering after fermentation of egg, cooked beans and chicken entrails for 7-8 days at 25-35 DEG C; and the nutrient solution, the animal and vegetable decomposition liquid and water are compounded at the proportion of 3:1:16. By spraying the insect attractant onto jujube flowers in the flowering stage, the proportion of fly flower-visiting insects in the total flower-visiting insect number is averagely increased by 5-8 percent. The insect attractant can be sprayed to achieve a remarkable trapping effect on flies and bees, the number and the flower-visiting frequency of flower-visiting insects are increased in a unit time, and the pollination and fertilization rates of jujube flowers are increased. After spraying of 100 times and 200 times of the insect attractant, the single-plant yieldsof the jujube tree are increased by 56.94% and 35.08% respectively, the prune single-plant yields are increased b6 36.36% and 25.38% respectively, and the almond single-plant yields are increased by48.11% and 44,30% respectively.

Disclosed are methods and probiotic compositions for the treatment of a metabolic disease or disorder such as, e.g., obesity, type 2 diabetes, or fatty liver. In some embodiments, heat-inactivated Parabacteroides goldsteinii is enterically administered to a subject, such as a human patient, to treat the metabolic disease or disorder or to promote the development of warm microbiota to treat the metabolic disease or disorder. In some aspects, spermine or spermidine may be administered to a subject or used in vitro to promote the growth of microbiota that can be used for the treatment of a metabolic disease or disorder.

The present invention is a method for the biosynthesis of hundreds of compounds, mainly found in the cannabis plant. The starting material for these compounds can be any biological compound that is used / produced in a biological organism from the sugar family starting materials or other low cost raw materials processed via enzymes or within organisms to give final products. These final products include, but are not limited to: cannabinoids, terpenoids, stilbenoids, flavonoids, phenolic amides, lignanamides, spermidine alkaloids, and phenylpropanoids. Specifically, the present invention relates to the regular / modified / synthetic gene(s) of select enzymes are processed and inserted into an expression system (vector, cosmid, BAC, YAC, phage, etc.) to produce modified hosts. The modified host is then optimized for efficient production and yield via manipulation, silencing, and amplifying inserted or other genes in the host, leading to an efficient system for product.

The invention provides a polyamine composite agent enhancing wheat and corn drought resistance. The composite agent is composed of the following components, by weight: 7-280 parts of putrescine, 0.4-10 parts of spermidine, 0.8-16 parts of spermine, 1-15 parts of succinic acid, and 6-150 parts of chitin. According to the invention, the weighed components are dissolved into 50000-150000 parts of water, such that the polyamine composite agent is obtained. Compared with prior arts, formerly commonly used spraying agent components are broken through. While the drought resistance of wheat and corn is improved, polyamine can be absorbed by plants according to agriculturally applied concentrations, such that no residue is left in soil. Even the polyamine absorbed by plants enters human and livestock bodies, the concentrations are greatly lower than those synthesized by human and livestock. The composite agent is harmless to human and livestock, and has a relatively low cost.

The invention belongs to the technical field of plant growth conditioning agents, and relates to a method for preparing a cold-resistance wheat conditioning agent. The method comprises the following steps: firstly, performing microwave degradation on enteromorpha polysaccharide prepared by using a hot water extraction method to prepare low molecular weight enteromorpha polysaccharide, further dissolving the prepared low molecular weight enteromorpha polysaccharide into distilled water so as to condition the concentration of enteromorpha polysaccharide to be 100-1000mg / L and prepare an enteromorpha polysaccharide solution, namely, the cold-resistance conditioning agent; or respectively adding salicylic acid, brassinolide, glycine betaine and / or spermidine into the enteromorpha polysaccharide solution, wherein the concentration of salicylic acid is 0mg / L or 100-200mg / L, the concentration of brassinolide is 0mg / L or 0.01-0.1mg / L, the concentration of glycine betaine is 0mg / L or 100-1000mg / L, and the concentration of spermidine is 0mg / L or 10-100mg / L, and finally uniformly stirring to prepare a compound solution, namely, the cold-resistance conditioning agent; or further adding a surfactant into the compound solution, wherein the concentration of the surfactant is 1000-20000mg / L, uniformly stirring so as to prepare the cold-resistance conditioning agent. The cold-resistance conditioning agent has the advantages of rich raw material resource, scientific preparation component, simple preparation process and low machining cost.

The invention relates to biphenyl aromatic hydrocarbon, water-soluble biphenyl aromatic hydrocarbon and a preparation method thereof. The biphenyl aromatic hydrocarbon adopts the structural formula as follows: FORMULA, wherein n is 4-13. According to invention, a thermostatic calorimetric titration experiment (ITC) measures that the bonding constant of a macrocyclic host CBP5 and a spermidine molecule is (1.5 plus or minus 0.2)*10<6>M<-1>. The preparation method is easy to operate and mild in reaction condition, and is suitable for industrial production; the stability of a medicine is improved, so that the medicine is higher in medicinal value.

The invention discloses a soapberry cutting seedling method and belongs to the technical field of plant vegetative propagation. A soapberry one-year-old bearing branch is used as a cutting shoot, soaking in a mixed solution with the concentration of 100 mg / L of ABT number one rooting powder, naphthylacetic acid and spermidine is carried out, and then the branch is inserted into a mixed matrix of yellow soil, herb-residue-containing decomposition organic fertilizer and vermiculite for seedling culturing. The one-year-old bearing branch is used as the cutting shoot, the survival rate is high, and the requirement on improved variety of raw material forest construction can be effectively relieved. The mixed matrix of the yellow soil, the herb-residue-containing decomposition organic fertilizer and the vermiculite is used for cutting, a cutting shoot root system is developed, coppice shoot growing is vigorous, the number of cutting shoot rotting phenomena is small, the cutting shoot is subjected to soaking in the mixed solution of the ABT number one rooting powder, the naphthylacetic acid and the spermidine, cutting shoot callus forming is quick, early rooting can be achieved, and the number of formed adventitious roots is large. The technology provides supporting for soapberry professionalization and large-scale vegetative propagation, and great significance is achieved in soapberry five breed promotion and early high yield.

The invention discloses a medicinal composition, which comprises two or three of a, b and c, wherein a is long-chain fatty acid compounds of a formula I or plant extract containing the same; b is flavonoid compounds of a formula II or plant extract containing the same; and c is the following alkaloid compounds or plant extract containing the same: N1,N5,N10-tri-(E / Z)-coumaroyl spermidine, 1-O-(beta-D-glucose)-(2S, 3S, 4R)-2N-[(2'R)-2'-hydroxytetracosenoicacid]-octadecylene-3,4-diol, putrescine and indol-3-acetic acid. The medicinal composition of the invention can be used for preparing aromatizing enzyme inhibitor, 5alpha reductase inhibitor, alpha1-adrenergic receptor antagonist, cyclooxygenase-2 inhibitor, prostate-specific antigen (PSA) secretion inhibitor, anti-inflammation medicamentor medicament for resisting prostatic hyperplasia, prostatitis or prostatic cancer.

The present invention provides methods for improving the specificity of nucleic acid amplification comprising incubating a nucleic acid molecule with a polymerase-Sso7 DNA binding domain conjugate and arginine, spermidine, or spermine. The present invention also provides reaction mixtures and kits for improving the specificity of nucleic acid amplification.

The invention, relating to the technical field of medicine, relates to a method for detecting the activity of spermidine / spermine N1-acetyltransferase, that is, a quantitative technique of analyzing SSAT activity through detecting acetylated metabolites of the SSAT substrate. According to the invention, the N-acetylation is regulated mainly through or only through SSAT in SSAT pathway and the specific substrate in the N-acetylation regulation is L-dopa, partial metabolism of the substrate can generate an acetylated metabolite N-acetyl-L-dopa thought the introduction of SSAT, the SSAT upregulation is positively correlated with cancer, accordingly, L-dopa can be used as a cancer mark regulated by the SSAT upregulation, and a novel detection method is provided for early detection, early diagnosis, early treatment and the like of tumors. The method has the advantages of safely, efficiency, strong practicality, convenient and fast operating method, simple usage, high positive incidence, and remarkable effect, and can be used as an auxiliary means for preventing, diagnosing, detecting, protecting, treating and studying various tumors.

The invention relates to a method for suppressing lignification of fruits and vegetables by multilayer coating preservation, and belongs to the technical field of fruit preservation. The method comprises the following steps: uniformly mixing hemicellulose, glucomannan, soy protein isolate and xanthan gum to form a film-forming substrate, then mixing the film-forming substrate with water, adding glycerin and / or potassium sorbate, and cooling the mixture to room temperature for later use to prepare film liquid A; immersing the fruits and vegetables in the prepared film liquid A for 30 s to 2 min, drying by cold air or at room temperature; adding salicylic acid, spermidine and spermine into the film liquid A, uniformly mixing to obtain film liquid B, immersing the treated fruits and vegetables in the film liquid B for 30 s to 2 min, drying by cold air or at room temperature; adding a bacteriostatic agent natamycin or epsilon-polylysine into a chitosan solution to obtain film liquid C, immersing the treated fruits and vegetables in the film liquid C for 30 s to 2 min, and drying by cold air or at room temperature. The method adopts multilayer coating preservation treatment to effectively suppress the formation speed of fruit and vegetable lignin.

The invention relates to the use of spermidine or a pharmaceutically acceptable derivative thereof as the active principle in a pharmaceutical, cosmetic or dietetic composition. The composition is used for promoting pigmentation of the hair, particularly the shaft of the hair. The invention also relates to the composition which promotes this pigmentation effect, the composition containing spermidine or a derivative thereof (such as a salt) as an active principle and is intended for topical or oral administration.