210 results about "Spermidine" patented technology

The invention discloses an immunoregulation hydrogel as well as a preparation method and application thereof, and relates to the technical field of medical materials, amino-derived cationic polysaccharide, carboxyl-derived anionic polysaccharide and dopa-modified protein biopolymer materials are used as biological matrix materials, spermidine is used as a bioactive functional component, and the immunoregulation hydrogel is prepared by a one-step method. The preparation method comprises the following steps: by taking a benzaldehyde-terminated high polymer material as a cross-linking agent, forming a first layer of dynamic reversible cross-linked network structure through Schiff base reaction; then calcium ions are introduced, and the calcium ions and carboxylate radicals are complexed and cross-linked to form a second layer of static irreversible cross-linked network structure which can be used for an ESD (Electro-Static Discharge) operation. By adopting a step-by-step cross-linking mode, the first-step cross-linked single-network hydrogel has injectability, self-repairability, tissue adhesiveness and an immune regulation function, and the second-step cross-linking further ensures stable and lasting performance, so that the problems that a submucosal injection material is easy to disperse, short in lasting time and poor in stability are solved. The immunoregulation function effect of the wound repair material is poor.

The invention discloses nucleic acid nano microspheres as well as a preparation method and application thereof, and belongs to the technical field of nano delivery. The preparation method comprises the following steps: carrying out first mixing on nucleic acid and part of water, and carrying out pretreatment and curing to form nucleic acid semi-solid gel; melting the nucleic acid semi-solid gel at 45-80 DEG C, and adjusting the pH value to 4-6 to form a phase A; carrying out second mixing on the spermidine-containing plant extract and the remaining water, and adjusting the pH value to 4-6 to form a phase B; and carrying out third mixing on the phase A, the phase B and an auxiliary agent to form the nucleic acid nano-microsphere, the nucleic acid nano-microsphere is prepared from the following components in percentage by mass: 0.5 to 8 percent of nucleic acid, 0.1 to 7.5 percent of a plant extract containing spermidine, 0 to 6 percent of an auxiliary agent and 78.5 to 99.4 percent of water, the mass ratio of the nucleic acid to the plant extract containing spermidine is (1-10): 1. The nucleic acid nano-microsphere has improved transdermal effect and stability, and can be used for transdermal drug delivery.

A nutraceutical composition, consisting of Nicotinamide Mononucleotide in a range 250-1000 mg, Spermidine in a range 0.5-10 mg, Rhodiola Rosea in a range 200-600 mg, Cacao in a range 1000-10,000 mg, L-Theanine in a range 50-200 mg, Glucosamine in a range 500-3000 mg, and Hyaluronic acid in a range 50-240 mg, or consisting of Epigallocatechin-3-gallate in a range 100-500 mg, Quercetin in a range 250-1000 mg, Resveratrol in a range 150-1000 mg, Sulforaphane in a range 1-50 mg, and Calcium Alphaketoglutarate in a range 300-2000 mg, or consisting of Microdose Lithium in a range 1-5 mg, Trimethylglycine in a range 500-2000 mg, Glutathione in a range 250-1000 mg, Fisetin in a range 100-1000 mg, Urolithin A in a range 250-2000 mg, Sodium Butyrate in a range 100-3000 mg, Berberine in a range 500-1500 mg, Apigenin in a range 50-500 mg, Cinnamon in a range 1000-6000 mg, and Malate in a range 1000-6000 mg.

The present invention relates to a preparation method of a wheat germ extract rich in spermidine. The method is characterized in that the method realizes the preparation of a wheat germ extract rich in spermidine from wheat germ by using macroporous adsorption resin combined with silica gel column chromatography technology. The raw material of the present invention is wheat germ for extraction, which is the raw material with the highest spermidine content in plants, providing the best choice for the preparation of a wheat germ extract rich in spermidine. And ethanol is used as the extraction solvent, which is simple, commonly used, easy to obtain and safe. Compared with other supercritical carbon dioxide, trichloroethanol, etc., the extraction cost is greatly reduced. The processing process of this method is simple, the preparation time is short, the extraction rate is high, and the thermal degradation of heat-sensitive components can be effectively avoided. The content of spermidine in the obtained wheat germ extract is 220 times that of wheat germ, which can effectively reduce the cost and increase the content of spermidine in the extract.

The present invention relates to the technical field of anti-aging compositions and particularly to an anti-aging composition and use thereof, and an anti-aging capsule and use thereof. The present invention provides an anti-aging composition comprising the following components: 20-2000 parts by mass of calcium α-ketoglutarate, 20-100 parts by mass of quercetin, 20-2000 parts by mass of trans-resveratrol, 20-100 parts by mass of calendulin, and 0.02-1.8 parts by mass of spermidine. The composition of the present invention has a multi-target synergistic effect on 12 major causes of aging and can safely and effectively reverse aging, enhance the physiological function, reduce the risk of chronic diseases, and improve the quality of life. The components of the composition are safe, and are carefully selected natural food ingredients; therefore, the composition is safe and reliable, and has no side effects with long-term use. The composition is prepared in an oral dosage form, so that the composition is convenient to administer, which improves patient compliance; moreover, the mode of application of the composition can be expanded.

A method of improving and / or enhancing at least one of bone mineralization, bone strength, bone mass, and bone mineral density in an individual in need thereof, the method comprising administering to the individual a composition comprising one or more anabolic amino acids, and the composition further comprising one or more autophagy-inducing compounds. In one embodiment, the one or more autophagy-inducing compounds are selected from the group consisting of thymol, carvacrol, spermidine, urolithin (e.g., Urolithin A, B or D), rapamycin, Torin1, valproic acid, polyphenols (e.g., resveratrol), caffeine, metformin, 5′ AMP-activated protein kinase (AMPK) activators, L-type calcium channel inhibitors, ketones (e.g. beta-hydroxybutyrate, ketone salts, or ketone ester derivatives), 4,4′-dimethoxychalcone and mixtures thereof.

The invention discloses an anti-aging composition which comprises the following components or consists of the following components in parts by weight: 10-20 parts of pyrroloquinoline quinone or derivatives thereof; 2 to 30 parts by weight of ergothioneine; 0.2 to 10 parts by weight of spermidine; and 10-650 parts by weight of a pomegranate extract. The invention also discloses an application of the anti-aging composition. According to the anti-aging composition disclosed by the invention, the pyrroloquinoline quinone or the derivative thereof, the ergothioneine, the spermidine and the pomegranate extract contained in the anti-aging composition can generate a synergistic effect, so that the anti-aging composition has good anti-oxidation and anti-aging effects. The anti-aging composition can be applied to food, health care products and medicines, and has the effects of resisting skin oxidation and delaying aging.

The invention belongs to the technical field of natural medicines, and particularly relates to application of spermidine alkaloid in preparation of anti-tumor medicines and / or reversion of drug resistance. The invention provides an application of macrocyclic spermidine alkaloid in preparation of anti-tumor drugs and / or reverse drug resistance. The macrocyclic spermidine alkaloid comprises one or more than two of 13-oxo-furan celastrus orbiculatus, furan celastrus orbiculatus, benzene-substituted celastrus orbiculatus and celastrus orbiculatus cinnamamide alkali. The macrocyclic spermidine alkaloid disclosed by the invention has the characteristics of broad-spectrum anti-tumor effect, good anti-tumor effect, relatively low toxic and side effects, capability of reversing 5-fluorouracil drug resistance, relatively high water solubility, relatively easy source, simple structure, only one chiral carbon in the structure, relatively easy artificial synthesis, intervention in cell autophagy mechanism and the like, so that the overall treatment cost is relatively low; the method has a potential important clinical application prospect.

The invention belongs to the field of cosmetics, and particularly discloses an ergothioneine anti-aging skin care composition and a preparation method thereof. According to the formula, each 100 g of the anti-allergy anti-inflammatory and anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti-allergy anti The transdermal rate and the stability of the ergothioneine and the pyrroloquinoline quinone are improved by adopting a liposome encapsulation technology, an'enzymatic + non-enzymatic 'dual-mode antioxidant system is formed by combining the French muskmelon extract and the roxburgh rose extract, full-type free radical scavenging is covered, and cell autophagy is regulated and controlled through spermidine to delay senescence.

Described herein, inter alia, are compositions comprising and methods of making and using endothelial cells (ECs) comprising one or more mitophagy-inducing agents (e.g., rapamycin, SKF-96365, lithium, carbamazepine, latrepirdine, spermidine, resveratrol, sodium valproate, trehalose, kaempferol, metformin, AICAR, nilotinib, ambroxol, curcumin, and VL-004). Also described herein, inter alia, are methods vascular cell therapy, promoting blood vessel formation, and increasing vascular development, angiogenesis, and cell junction via administration of a population of ECs comprising a mitophagy-inducing agent (MIA-ECs).

The invention discloses a fluorescent probe for detecting spermine and spermidine in a water sample and urine as well as a preparation method and application of the fluorescent probe, and relates to the technical field of supramolecular fluorescent probes, and the fluorescent probe is formed by reacting 14-membered cucurbit urils (tQ [14]) and a compound (E)-1-(4-boron benzyl)-4-(4-hydroxyl styryl) quinoline-1-ammonium bromide according to a molar ratio of 1: 1. The novel fluorescent probe provided by the invention can specifically detect spermine and spermidine in a water sample and urine, and the preparation method of the fluorescent probe is simple and easy to implement; the method for detecting spermine and spermidine in a water sample and urine by using the fluorescent probe has the advantages of high sensitivity, high selectivity, low cost, simple sample treatment, convenient operation, rapid determination and the like.

The present invention relates to a composition for modulating the release of biologically active aliphatic polyamines, said composition comprising palmitamide ethanol and preferably a sucrose ester as an agent for modulating the release of aliphatic polyamines from the composition. The composition may be used directly or added to a formulation of a supplement to promote hair growth.

The invention discloses a preparation method of an edible flower phenol amide compound and application of the edible flower phenol amide compound in prevention and treatment of non-alcoholic fatty liver disease, and particularly relates to the field of development of new application of food raw materials. The edible flower phenol amide compound N1, N5, N10-coumaroyl spermidine (TCS) can be green extracted by using a natural deep-eutectic solvent (NADES), and can inhibit free fatty acid-induced liver cell lipid accumulation and improve fatty acid-caused liver cell fatty degeneration; oxidative stress and inflammation induced by free fatty acid are inhibited, and expression of senescence-related markers is inhibited, so that development of NAFLD is effectively inhibited.

The invention discloses an oral hard capsule health-care product with an anti-aging function as well as a preparation method and application of the oral hard capsule health-care product. The health-care product is mainly prepared from eight active ingredients including EGT (Ergothioneine), Ca-AKG (alpha-ketoglutarate), PQQ (pyrroloquinoline quinone), fisetin, salidroside, urolithin A, spermidine and an apple stem cell extract. Through scientific compatibility of the various components, the composition disclosed by the invention can comprehensively intervene in a cell aging process through a synergistic effect of a plurality of pathways of enhancing the cell antioxidant capacity, improving the mitochondrial function, activating cell autophagy, promoting cell regeneration and repair and the like. The invention also provides a preferred content range of the health-care product, a preparation method of the health-care product and application of the health-care product in preparation of health-care foods for delaying senescence, improving cell viability or improving mitochondrial functions. The product provided by the invention has the characteristics of multiple targets and synergistic interaction, and provides a new effective scheme for anti-aging intervention.

The invention relates to the technical field of forest cultivation, and discloses a carya illinoensis fruit forest variety configuration method which comprises the following steps: configuring a main cultivation variety and a pollination variety in a fruit forest; preparing a pollination synergistic composition; the composition is sprayed at the initial stage of stigma exposure of female flowers of a main cultivated variety. The composition comprises a phenolic acid grafted carboxymethyl chitosan derivative, low methoxyl pectin, a calcium ion cross-linking agent, sugar alcohol chelated boron and spermidine. According to the method, an interfacial film with antioxidant activity is formed on the surface of the stigma by using the grafting modified matrix, so that the stigma browning is effectively inhibited, and the pollination period is prolonged; by constructing a calcium cross-linked low-methoxyl pectin interpenetrating network, an interfacial film is endowed with an intelligent degradation characteristic of enzyme-sensitive response to pollen, and protection at ordinary times and immediate dissolution after touching flowers are realized. According to the method, pollen is captured through static electricity, nutritional factors are released in a targeted mode, the pollination and fertilization efficiency is remarkably improved, the sterility risk in the flowering phase is relieved, and the yield and quality of carya illinoensis are improved.

The application discloses a method for detecting plant free polyamines by HPLC, which can clearly separate the target substances putrescine (Put), spermidine (Spd), thermospermine (T-Spm) and spermine (Spm) in a plant sample. The method has stable retention time of the separated target substances, and the peaks corresponding to the target substances separated from different samples and the standard sample can be well overlapped, data is clear, and the result is reliable. The separation time is shortened by half, the use amount of the mobile phase is halved, the operation cost is greatly reduced, the waste liquid is halved, the recovery cost of the waste liquid is also halved, and the potential pollution to the environment is reduced.

The invention discloses application of spermidine in in-vitro culture of human unfrozen oocytes and embryos. By adding the combination of spermidine, melatonin and astaxanthin into the thawed oocyte resuscitation culture solution and the embryo in-vitro culture solution, the mitochondrial autophagy and mitochondrial functions of the thawed oocytes are improved, so that the in-vitro culture effect of the thawed oocytes and embryos is improved, and the success rate of an assisted reproduction technology is increased.

The invention discloses application of spermidine in preparation of a preparation for improving fertility of female mammals, and belongs to the technical field of reproductive biology. According to the invention, spermidine with different concentrations is introduced into mice, and the effect of spermidine on ovarian hormone secretion, ovarian reserve level and follicle number of female mammals is determined in a diet intake manner, so that the fertility of female animals is improved; it is found that spermidine can effectively improve the ovarian function, increase the secretion level of E2, P4, LH and AMH hormones closely related to the ovarian function, reduce the number of atresia follicles of the ovarian and increase the litter birth number of female mice by 1-2, and the spermidine has good animal breeding and livestock raising application prospects.

The present invention provides a method for preparing spermidine, which relates to the technical field of organic synthesis. The method for preparing spermidine comprises: condensing 3-chloropropylamine hydrochloride and triphenylmethane to obtain 3-chloro-N-tritylpropylamine; condensing 3-chloro-N-tritylpropylamine and 1,4-butanediamine to obtain N 1 ‑(3‑(tritylamino)propyl)butane‑1,4‑diamine; N 1 The trityl group is removed from 1,4-(3-(tritylamino)propyl)butane to obtain spermidine hydrochloride; and spermidine hydrochloride is liberated in the presence of a base to obtain spermidine. The preparation method of the present invention can achieve a high yield and purity in a relatively short reaction period, avoids the problems of a high proportion of by-products, high purification difficulty, and residual solvent, improves the reaction efficiency, and has mild reaction conditions, simple production equipment, and is more energy-saving and environmentally friendly while being convenient for industrialized amplification production.

The invention discloses a composition for promoting bulb expansion of hippeastrum hippeastrum, the composition is an aqueous solution, the solute in the composition comprises putrescine, spermidine, spermine, triacontanol, ethanol, Tween 80, sodium lignin sulfonate and ethylene glycol, and the dosage ratio of the materials is 10: 1: 2: 0.05: 10: 5: 3: 2 according to the dosage weight. And diluting the composition with water to obtain a diluent with the mass percent concentration of 0.04-0.06%. The diluent is used for foliage spraying and seedball base irrigation in the germination stage, the growth stage, the flowering stage and the post-flowering rejuvenation stage of hippeastrum hippeastrum, the seedball diameter of hippeastrum hippeastrum can be remarkably increased, and economic benefits are increased.

This invention discloses a kit and method for extracting cell nuclei from plant tissues, belonging to the field of biotechnology. The kit includes a nuclear lysis buffer and a nuclear washing buffer. The nuclear lysis buffer is prepared using nuclease-free water with sucrose, Tris-HCl (pH 7-8), NaCl, MgCl2, a surfactant, DTT, an RNase inhibitor, and spermidine. The nuclear washing buffer is prepared using nuclease-free water with sucrose, Tris-HCl (pH 7-8), NaCl, MgCl2, DTT, an RNase inhibitor, and spermidine. This kit is suitable for extracting cell nuclei from fresh yew stem tissue, yielding cell nuclei with intact nuclear membranes, stable gene expression, high purity, and few impurities, suitable for single-cell sequencing.

Disclosed herein are nutritional compositions aimed at promoting longevity and wellness. The compositions are formulated for oral intake and include a variety of nutrients and functional compounds such as proteins, creatine, antioxidants, vitamins, minerals, hyaluronic acid, biotin, CoQ10, spermidine, and one or more NAD+ precursors. These components may serve to enhance muscle health, antioxidant function, skin, hair and joint health, cellular and cardiovascular health, thus contributing to the overall vitality and lifespan of an individual.

The invention discloses an application of N1, N10-dicaffeoyl spermidine in preparation of a medicine for preventing and treating atherosclerosis. Belongs to the technical field of biological medicine. When the N1, N10-dicaffeoyl spermidine is administered to an experimental animal within the dosage range of 10-25 mg / kg, the area of ApoE- / -mouse atherosclerotic plaque induced by high homocysteine (HCY) can be remarkably reduced, meanwhile, the HCY level, the levels of four blood lipid items (TG, TC, LDL-C and HDL-C) and the levels of inflammatory factors such as IL-6, IL-1beta, TNF-alpha, MCP-1 and CRP in serum of the atherosclerotic mouse can be remarkably callback, and the N1, N10-dicaffeoyl spermidine can be used for treating atherosclerotic mice. All the results prove that the N1, N10-dicaffeoyl spermidine has the effect of improving the atherosclerosis and can be used for preventing and treating cardiovascular system diseases such as the atherosclerosis.

This invention relates to the field of functional food technology, and more particularly to the application of safflower honey, or safflower honey extract, in enhancing the activity of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH). The invention has found that safflower honey, or safflower honey extract, can effectively enhance ADH and ALDH activity, thereby promoting alcohol metabolism and achieving a hangover-relieving effect. Further research on the extract revealed that a mixture of syringaldehyde, riboflavin, lutein, luciferin, and three p-coumaroyl spermidine plays a key role in enhancing ADH and ALDH activity, thus providing an application of this mixture in hangover relief. The application of safflower honey and its extract provided by this invention has high application value for developing foods and health products with hangover-relieving effects.

The invention discloses a medical dressing capable of absorbing moisture and releasing sweat and a preparation method of the medical dressing, and relates to the technical field of medical dressings, the medical dressing comprises a polyurethane copolyester nanofiber membrane, and the preparation method of the polyurethane copolyester nanofiber membrane comprises the following steps: pouring non-isocyanate polyurethane copolyester, levofloxacin and polyacrylonitrile into hexafluoroisopropanol, and stirring uniformly; carrying out electrostatic spinning; and spin-coating a chitosan-gelatin-glycerol mixed solution, and spin-coating a spermidine cross-linking agent to obtain the polyurethane copolyester nanofiber membrane. By introducing the polyglycolic acid-based non-isocyanate polyurethane copolyester and the levofloxacin, the levofloxacin is uniformly dispersed and embedded into a fiber network, so that uniform dispersion and controllable release of an antibacterial drug are realized, growth and reproduction of bacteria at a wound are effectively inhibited, and the problems of wound infection and slow healing are solved; the healing speed is increased; and the complication risk is reduced.

The invention provides an anti-aging composition rich in cycloastragenol and capable of prolonging telomeres. The composition comprises the following active components in parts by weight: 10-30 parts of cycloastragenol, 5-15 parts of nicotinamide mononucleotide, 1-5 parts of spermidine hydrochloride and 40-60 parts of a phospholipid carrier. According to the invention, through triple synergistic effects of cycloastragenol, nicotinamide mononucleotide and spermidine hydrochloride, an optimized enzymolysis-supercritical extraction process and a composite phospholipid carrier system are combined, so that the telomere extension effect, the component stability and the intestinal absorption rate are remarkably improved; and an integrated solution of multi-target anti-aging, efficient extraction and safe delivery is realized.

The invention relates to a growth regulator for improving the drought resistance of wheat in the booting stage, a preparation method and application, and the growth regulator takes a composition containing spermidine, 2, 4-epibrassinolide and monopotassium phosphate as an active ingredient. According to the growth regulator for improving the drought resistance of wheat in the booting stage, the drought resistance of wheat in the booting stage can be improved, and the drought resistance of wheat in the later growth stage can be greatly improved; moreover, spermidine, 2, 4-epibrassinolide and monopotassium phosphate are specifically combined and proportioned and are applied to improve the drought resistance of wheat at the booting stage for the first time, the three active ingredients have a synergistic effect, the comprehensive drought resistance of wheat can be remarkably improved, so that the grain yield is stabilized, and meanwhile, the composition has the characteristics of safety, high efficiency and environmental protection, and is worthy of popularization and application. The preparation method is simple and easy to implement, and is convenient to popularize and apply.

Described herein are compositions for maintaining and / or improving bone health in a subject (e.g., an aging subject) comprising N 1 - dihydrocaffeoyl-N 10 - caffeoyl spermidine and an additive. Further, described herein are methods for maintaining and / or improving bone health in a subject (e.g., an aging subject). Further, described herein are methods for preventing and / or treating bone disease in a subject (e.g., an aging subject).

The invention discloses a cutting seedling rooting agent and a preparation method thereof, and relates to the technical field of garden horticulture, and the cutting seedling rooting agent comprises the following raw materials by weight: 5-15% of a citrus pectin oligosaccharide-spermidine compound, 0.0001-0.0005% of a brassinolide nanoemulsion, 2-5% of proline, 3-8% of sugar alcohol calcium, 5-10% of modified attapulgite, 0.25-0.55% of a surfactant, and the balance of deionized water. After use, the rooting effect is good and the stability is high.

The invention provides an anti-skin aging composition and application thereof, and belongs to the technical field of cosmetics. The composition comprises spermidine and ferulic acid, the mass ratio of the spermidine to the ferulic acid is 1: (0.5-3), and the composition has a remarkable anti-aging effect. Tests prove that the composition provided by the invention can obviously promote the secretion of collagen and promote the growth of human skin fibroblasts, and also has relatively strong oxidation resistance. Moreover, spermidine can enhance the ferulic acid uptake efficiency of skin cells, ferulic acid can stabilize the activity of spermidine, and spermidine and ferulic acid cooperate to comprehensively regulate aging from cell and molecule levels, so that an efficient and stable multi-mechanism anti-aging solution is provided, and the defects in the prior art are overcome.