The invention provides a method for preparing ACE (
Angiotensin Converting Enzyme) inhibitory peptides through bean pulp enzymolysis. The method comprises the following steps that (1) high-temperature bean pulp is crushed and sieved, obtained bean pulp
powder and water are mixed according to a
mass ratio of 1:(4-6), the pH value is regulated to 8 to 10, and a bean pulp mixture is obtained; (2) the pH value of the bean pulp mixture is regulated to 7 to 9, a complex
enzyme preparation is added, the mixture is uniformly mixed,
hydrolysis is carried out for 5 to 8 hours at the temperature being 35 to 50 DEG C, after the
hydrolysis is completed,
hydrolysis liquid is boiled in a boiling water bath for
enzyme deactivation, and mixed liquid is obtained; (3) the pH value of the mixed liquid is regulated to 6.5 to 7.5, supernatant is taken,
ultrafiltration treatment is performed through an
ultrafiltration membrane, and filtered liquid is obtained; and (4) the filtered liquid is subjected to vacuum freezing
drying, and the
ACE inhibitory peptide powder is obtained. The method for preparing
ACE inhibitory peptides through bean pulp enzymolysis has the advantages that the process is simple and is easy to implement; in the whole process, the
reaction conditions are mild; the compounded complex
enzyme preparation is used; and the industrial
processing operation is convenient.