360 results about "Inhibitory peptide" patented technology

The invention discloses a xanthine oxidase inhibitory peptide with a kidney improving function and application thereof, and belongs to the technical field of biological medicines.The xanthine oxidase inhibitory peptide is screened out by conducting enzymolysis, separation and purification on litopenaeus vannamei processing by-products and utilizing a molecular docking technology, the amino acid sequence of the xanthine oxidase inhibitory peptide is Pro-Leu-Gly-Pro-Pro-Pro, and the amino acid sequence of the xanthine oxidase inhibitory peptide is Pro-Leu-Gly-Pro-Pro-Pro. And the half inhibition concentration of the polypeptide is 2.356 + / -0.2448 mM. The xanthine oxidase inhibitory peptide is proved to have an improvement effect on hyperuricemia mice, the improvement effect is mainly characterized by reducing the content of serum uric acid, creatinine and urea nitrogen of the hyperuricemia mice, inhibiting the activity of xanthine oxidase (XOD) in serum and livers of the mice, inhibiting synthesis of uric acid and improving kidney injury of the mice, and Toxinpred prediction shows that the xanthine oxidase inhibitory peptide has the effect of inhibiting the activity of xanthine oxidase (XOD) in the serum and livers of the mice. The xanthine oxidase inhibitory peptide is free of toxic and side effects, so that the xanthine oxidase inhibitory peptide has a wide application prospect in preparation of food or drugs for improving renal functions.

The invention belongs to the field of bioactive peptides. The invention provides an angiotensin converting enzyme inhibitory peptide as well as a screening method and application thereof. The amino acid sequence of the inhibitory peptide is GKGLW and / or GDGLKW. According to the screening method of the ACE inhibitory peptide, the soybean protein is taken as a raw material, and the ACE inhibitory peptide is prepared and screened by adopting a targeted alkaline protease and chymotrypsin double-enzymolysis technology, so that rapid screening of the ACE inhibitory peptide is realized, and an experimental basis is provided for the structure-function relationship of the ACE inhibitory peptide. The inhibitory peptide provided by the invention has strong antihypertensive activity, high safety and no toxic or side effect. Digestive tract stability determination shows that the inhibitory peptide has good gastrointestinal tract stability in gastrointestinal tract digestion, and can still keep good activity after entering blood.

The invention particularly relates to a method for extracting ACE (Angiotensin Converting Enzyme) inhibitory peptide from tilapia skin. The tilapia skin is a by-product rich in collagen, and has important development significance as a screening raw material of the ACE inhibitor. The invention provides a method for extracting an ACE (Angiotensin Converting Enzyme) inhibitor by taking tilapia skin as a raw material, which comprises the following steps of: degreasing the tilapia skin, crushing, adding acid liquor, extracting to obtain fish skin collagen crude protein, adding enzyme into the fish skin collagen crude protein for enzymolysis, and reserving the part with the molecular weight of less than 3kDa in an enzymolysis product, namely the ACE inhibitory peptide. Particularly, the enzyme is a product of bacillus subtilis or bacillus coagulans under gelatin-induced fermentation.

The invention belongs to the field of skin care products, and particularly relates to an anti-wrinkle and anti-aging composition as well as a preparation method and application thereof, and the anti-wrinkle and anti-aging composition comprises neurotransmitter inhibition peptide, signal peptide, madecassoside, inositol and glycosylglycerol. The neurotransmitter inhibition type peptide is at least three of acetyl hexapeptide-8, arginine / lysine polypeptide, dipeptide diaminobutyrylbenzylamide diacetate and acetyl octapeptide-3, and the signal type peptide is at least two of palmitoyl pentapeptide-4, decapeptide-4 and acetyl tetrapeptide-9. Compared with the prior art, all the components of the anti-wrinkle and anti-aging composition act on different anti-aging pathways and can be matched with one another from multiple aspects of oxidation resistance, inflammation resistance, moisturizing, cell viability enhancement, wrinkle reduction and the like, a more comprehensive and better anti-aging effect is provided, skin aging is comprehensively resisted, the skin is in a more young state, and the anti-wrinkle and anti-aging composition has the advantages that the anti-wrinkle and anti-aging effects are achieved. And the anti-wrinkle and anti-aging composition is small in irritation and good in stability.

The invention discloses a method for preparing, enriching and separating various egg yolk bioactive peptides through enzymolysis of defatted egg yolk powder and application of the egg yolk bioactive peptides, and belongs to the technical field of egg product processing and bioactive peptides. Egg yolk protein hydrolysate is prepared by adopting a three-step enzymolysis process, adsorption and gradient elution are performed on the egg yolk protein hydrolysate by adopting a macroporous resin chromatographic column, four components including water-1, water-2, 20% ethanol and 80% ethanol are obtained, the water-1 component is common egg yolk peptide, the water-1 component is enriched with osteogenic active peptide, the water-2 component is enriched with ethanol, and the 80% ethanol is enriched with ethanol. The 20% ethanol component enriches antioxidant peptide, and the 80% ethanol component enriches ACE inhibitory peptide. The functional activity of each bioactive peptide component is very strong, and the bioactive peptide component can be used as a high-end functional ingredient. According to the method disclosed by the invention, comprehensive utilization of the degreased yolk powder is realized, enrichment and separation of three active peptides are realized by using one raw material through a step-by-step enzymolysis-directional separation technology, the yield of the active peptides is greatly improved, and the economic benefits of enterprises are improved.

The invention belongs to the technical field of biological medicines, and discloses an angiotensin I converting enzyme inhibitory peptide. According to the present invention, the action mechanisms of two ACE inhibitory peptides H5 (LIIPQH, 719.85 Da) and H6 (LIIPEH, 720.85 Da) extracted, separated and purified from rice wine residue are researched, and the in vitro activity of the ACE inhibitory peptides is researched through an EA.hy926 cell model, such that the two ACE inhibitory peptides can provide the activity effect by promoting the release of NO, the expression of eNOS and SOD, and inhibiting the release secretion of ET-1 and the release of ROS; meanwhile, the cells are subjected to transcriptomics analysis, and the pathway mechanism of polypeptide action is defined. The ACE inhibitory peptide is expected to serve as a potential antihypertensive drug or a functional food ingredient, the high-value utilization of the rice wine residues is improved, and a new application way is provided for the food-borne ACE inhibitory peptide in the aspect of treating hypertension.

The invention provides ACE (Angiotensin Converting Enzyme) inhibitory peptide, middle-aged and elderly formula goat milk powder and a preparation method thereof, goat milk is taken as a raw material, the goat milk is sterilized and cooled, compound protease consisting of three proteases is inoculated for constant-temperature enzymolysis, and the ACE inhibitory peptide is obtained through ultrafiltration, preparative chromatographic separation and freeze drying. And uniformly mixing the active peptide with a nutrition enhancer and goat milk powder required by the middle-aged and elderly people to obtain the active peptide goat milk powder for the middle-aged and elderly people. Or after the goat milk is subjected to enzymolysis by compound protease, directly adding a nutrition enhancer required by the middle-aged and elderly people, and then concentrating and spray-drying to obtain the formula goat milk powder for the middle-aged and elderly people. The ACE inhibitory peptide prepared by the invention can be used as a food functional factor, and the prepared formula goat milk powder for middle-aged and elderly people contains the ACE inhibitory peptide and has the function of assisting in lowering blood pressure.

The invention discloses an ACE (Angiotensin Converting Enzyme) inhibition and DPP-IV (Dipeptidyl Peptidase-IV) inhibition bifunctional peptide as well as a design method and application thereof. The amino acid sequence of the bifunctional peptide fragment is Trp-Ala-Leu-Val-Ala-Pro or Trp-Pro-Leu-Val-Ala-Pro. According to the design method, hydrophobic amino acid replacement is carried out on most N-terminal first amino acid according to the characteristics of the DPP-IV inhibitory peptide, and N-terminal second amino acid is kept as proline Pro or alanine Ala. The difunctional peptide fragment obtained through the DPP-IV inhibitory activity optimization strategy has the effects of assisting in lowering blood pressure and lowering blood sugar at the same time and can be used for preparing functional food or health food with the functions of assisting in lowering blood pressure and lowering blood sugar, and the optimization strategy can be applied to improving the blood sugar lowering effect of the peptide fragment and has good market application prospects.

The invention discloses a hemp seed protein source pancrelipase inhibitory peptide as well as a preparation method and application thereof, and belongs to the technical field of bioactive peptides. The hemp seed protein source pancrelipase inhibitory peptide provided by the invention has an amino acid sequence as shown in SEQ ID NO. 1 (YNLPILRF). The cannabis sativa L. protein source pancreatic lipase inhibitory peptide provided by the invention is separated from cannabis sativa L. protein for the first time, is a novel cannabis sativa L. protein source pancreatic lipase inhibitory peptide, has good pancreatic lipase inhibitory activity, can be used as a natural anti-obesity agent in food, health care products and weight control related drugs, and has broad application prospects. Good application prospects are realized.

The invention discloses preparation and application of lotus seed protein peptide with ACE (angiotensin converting enzyme) inhibitory activity, and belongs to the technical field of food-borne active peptide development. According to the ACE inhibitory peptide WVPRYFPF and the preparation method thereof, the ACE inhibitory peptide is rapidly screened by utilizing a polypeptiomics and molecular docking technology, the defects of time consumption and labor consumption caused by step-by-step separation and purification are avoided, the obtained ACE inhibitory peptide WVPRYFPF has extremely high ACE inhibitory activity, IC50 of the ACE inhibitory peptide WVPRYFPF is 16.7 mu M, and the ACE inhibitory peptide WVPRYFPF can be used for assisting or replacing hypertension drugs. The lotus seed protein peptide disclosed by the invention has the characteristic of inhibiting ACE enzyme activity in vitro, and animal experiments prove that the protein peptide can also play a role in lowering blood pressure in a spontaneous hypertension rat (SHR).

An α-amylase inhibitory peptide. The peptide comprises amino acid sequences of eight adjacent amino acids X1, X2, X3, X4, X5, X6, X7, and X8, wherein each amino acid of the peptide is independently selected from a D-amino acid or an L-amino acid, and at least one of X2, X4, and X6 is W. The peptide has α-amylase inhibitory activity, has the effects of losing weight and lowering blood sugar, and can be used for preparing α-amylase inhibitors, weight-losing drugs, hypoglycemic drugs, or foods, health-care products or drugs for preventing or treating obesity-related diseases.

The invention relates to the technical field of biology, in particular to a DPP-IV inhibitory peptide extracted from buffalo cheese protein hydrolysate and application of the DPP-IV inhibitory peptide. An active component with the molecular weight smaller than 3 kDa is obtained through alkaline protease enzymolysis, ultrafiltration separation and LC-MS / MS identification, and the DPP-IV enzyme inhibitory activity IC50 value of the active component is 12.6 mg / mL. Four new DPP-IV inhibitory peptide fragments are found, are amino acid sequences in a sequence table SEQ ID NO: 1-4, have good DPP-IV inhibitory activity, and have good application prospects in being developed into functional foods or medicines for regulating blood sugar. In conclusion, the enzymolysis product and the peptide fragment can be prepared into antidiabetic drugs or functional foods, and have the advantages of being natural, safe and efficient. The invention provides a new strategy for high-valued utilization of buffalo milk resources and treatment of type 2 diabetes.

The technology described herein relates to methods of inhibiting Peptidoglycan Recognition Protein 1 (Pglyrp1) in order to improve immune-checkpoint inhibitor treatments for cancer and autoimmune diseases.

The invention discloses a polypeptide with ACE inhibitory activity and application thereof, and relates to the technical field of biology. The amino acid sequence of the polypeptide is as shown in SEQ ID NO. 1. The invention further provides application of the polypeptide in preparation of antihypertensive drugs, products beneficial to maintaining the healthy level of blood pressure and angiotensin converting enzyme inhibitors. The ACE inhibitory peptide in tuna blood is efficiently screened through technologies of pepsin and pancreatin enzymolysis, ultrafiltration, liquid chromatography-tandem mass spectrum sequence identification and the like, and the polypeptide with the amino acid sequence being YPWTQR is finally obtained through screening. The polypeptide has good ACE inhibition activity, and the IC50 value of the polypeptide to ACE can reach 46.29 [mu] mol / L. The polypeptide has the potential of being developed into antihypertensive drugs and products beneficial to maintaining the health level of blood pressure, and has high market application value.

The invention relates to the technical field of biology, in particular to an ACE inhibitory peptide, a preparation method and application, the ACE inhibitory peptide has the amino acid sequence of Trp-Pro-Phe (WPF) or Phe-Pro-Trp (FPW) or Phe-Gly-Trp (FGW) or Leu-Pro-Val-Pro-Ala-Phe-Asn (LPVPAFN) or Leu-Trp-Trp-Leu (LWWL), the ACE inhibitory peptide WPF, FPW, FGW, LPVPAFN and LWWL have outstanding antihypertensive activity, the IC50 values of the WPF, FPW, FGW, LPVPAFN and LWWL are 0.264 mg / mL, 0.800 mg / mL, 0.106 mg A variety of aquatic product peptide powder sources are separated and purified by an affinity ultrafiltration screening method, an ultrafiltrate component is selected for amino acid sequence identification, and a new amino acid sequence with ACE inhibitory activity is screened out; the ACE inhibitory peptides WFP, FPW, FGW, LPVPAFN and LWWL prepared by the invention have outstanding antihypertensive activity.

The present invention specifically relates to a method for extracting ACE inhibitory peptides from tilapia skin. Tilapia skin is a byproduct rich in collagen, and has important development significance as a raw material for screening ACE inhibitors. The present invention is designed to provide a method for extracting ACE inhibitors using tilapia skin as a raw material, comprising the following steps: defatting the tilapia skin and crushing it, adding acid to extract to obtain fish skin collagen crude protein, adding enzyme to the fish skin collagen crude protein for enzymolysis, and retaining the part of the enzymolysis product with a molecular weight less than 3kDa is the above-mentioned ACE inhibitory peptide; in particular, the above-mentioned enzyme is a product of Bacillus subtilis or Bacillus coagulans under gelatin-induced fermentation.

The invention discloses a tyrosinase inhibitory peptide from kelp as well as a preparation method and application of the tyrosinase inhibitory peptide. The preparation method comprises the following steps: pretreating kelp, fermenting the kelp by using lactobacillus plantarum, carrying out centrifugation, ultrafiltration and component identification on the obtained fermentation liquor to obtain the composition of kelp polypeptide, screening the tyrosinase inhibitory peptide through molecular docking, and finally carrying out solid-phase synthesis to obtain the tyrosinase inhibitory peptide prepared by the invention. The lactobacillus plantarum fermentation technology is simple and feasible, the production cost is low, equipment is easy to obtain, active ingredients in kelp can be released to the maximum extent, and the prepared polypeptide has the effect of inhibiting the activity of tyrosinase and can be used for developing a tyrosinase inhibitor.

The invention relates to a xanthine oxidase inhibitory peptide derived from green and sweet tuna and application of the xanthine oxidase inhibitory peptide, and belongs to the technical field of polypeptides, and the amino acid sequence of the xanthine oxidase inhibitory peptide is Trp-Thr-Ala (WTA), Trp-Val-Pro (WVP), Trp-Leu-Pro (WLP) or Trp-Gly-Phe (WGF). The invention also provides an application of the inhibitory peptide as or in preparation of a xanthine oxidase inhibitor and an application of the inhibitory peptide as or in preparation of a medicine with an effect of inhibiting uric acid level. The invention also provides a xanthine oxidase inhibitor containing at least one inhibitory peptide or a composition or functional food for inhibiting uric acid. The selected inhibitory peptide is a novel food-borne XO inhibitory peptide, is good in water solubility, non-toxic and good in small intestine absorption and blood brain barrier permeability, has the potential of serving as a xanthine oxidase inhibitor and reducing the uric acid level, and can be used for preparing a medicine for preventing and treating hyperuricemia or serving as a functional food additive.

The invention discloses a cubilose peptide with ACE inhibitory activity and a preparation method and application thereof, the cubilose peptide with the ACE inhibitory activity is prepared through enzymolysis, meanwhile, LC-MS / MS analysis is performed on peptides in enzymatic hydrolysate, a peptide fragment with potential ACE inhibitory activity is screened out in combination with a bioinformatics means, and the obtained peptide fragment has high ACE inhibitory activity through synthesis verification. The bird's nest ACE inhibitory peptide disclosed by the invention has a good application prospect in functional foods or health-care products and food-borne medicines.

The invention provides a spirulina-derived ACE (angiotensin converting enzyme) inhibitory peptide as well as a preparation method and application thereof, and belongs to the technical field of marine bioactive peptides. Spirulina phycobiliprotein is used for preparing the ACE inhibitory peptide of the spirulina source, five kinds of small molecule polypeptides including YATY, RYVTY, YVTY, KAYF and SPSWY are obtained through co-screening, the five kinds of polypeptides all have good ACE inhibitory activity, and the RYVTY activity is the highest. The spirulina-derived polypeptide prepared by the preparation method disclosed by the invention has high hydrolysis degree and high ACE inhibitory activity, can be applied to the fields of food, health care products, medicines and the like, and has a wide application prospect.

The invention discloses a TREM-1 inhibitory peptide nano-particle which is prepared by the following steps: by taking a dendritic polymer PAMAM as a carrier, coupling a TREM-1 inhibitory peptide with amino groups on the carrier by utilizing the characteristic of polyamino groups on the surface of the PAMAM, and coupling a brain-targeted peptide with the other part of amino groups on the carrier through MAL-PEG2000-NHS, so as to obtain the TREM-1 inhibitory peptide nano-particle. And modifying the residual amino groups on the carrier with PEG (Polyethylene Glycol) to obtain the nano-particles. The nanoparticles can relieve encephaledema, neuroinflammation injury and blood brain barrier damage caused by traumatic craniocerebral injury, improve cognitive impairment and long-term prognosis after trauma, and have a very high curative effect on clinical treatment of craniocerebral injury.

The invention discloses a tilapia skin DPP-IV inhibitory peptide as well as a preparation method and application thereof, and belongs to the technical field of food-borne active peptide development. The preparation method comprises the following steps: taking tilapia skin as a raw material, extracting gelatin from the tilapia skin through an acid-alkali treatment method, and then performing simulated gastrointestinal digestion on the gelatin by utilizing simulated digestive juice prepared from pepsin, trypsin and chymotrypsin to obtain a digestion product with DPP-IV inhibitory activity; the tilapia skin active peptide with relatively high DPP-IV inhibitory activity is obtained through further ultrafiltration and identification, amino acid sequences of the tilapia skin active peptide are AKVELLK, VPGPMGPMGPR and DGIR respectively, and the three inhibitory peptides have good DPP-IV inhibitory activity and can be used for developing food, health care products or medicines for assisting in regulating blood sugar.

The invention relates to a preparation method and application of yolk polypeptide with ACE inhibitory activity, and the method comprises the following steps: Q1, adding degreased yolk powder into a proper amount of water, dissolving, and adjusting the pH value to 6-8; and Q2, adding alkaline protease or flavourzyme to carry out enzymolysis for 1.5-2.5 hours under the conditions that the temperature is 50-60 DEG C and the pH value is 7-8, and then carrying out enzyme deactivation to obtain the egg yolk hydrolysate. The ACE inhibitor with high activity is prepared by adopting step-by-step enzyme hydrolysis, five ACE inhibitory peptides (KFIPL, KFLPGY, KFLPTF, APTFGKL and KLPDMILY) with high activity are screened and identified by utilizing a bioinformatics method, and in order to solve the problem that the polypeptide is unstable after being sterilized in the functional beverage, CMC and GG are used as stabilizers, so that the utilization problem of the enzymolysis polypeptide in the functional beverage is solved.

The invention relates to the technical field of biologic stimulant preparation, and discloses a biologic stimulant mixing preparation two-stage control method, which comprises: adopting dissolved oxygen gradient and carbon source pulse cooperative control, linearly reducing the concentration of dissolved oxygen in a fermentation tank from an initial value at a specific rate, and injecting a carbon source at regular time; when the flora density and the quorum sensing signal molecule concentration reach specific threshold values, triggering stage switching; starting pressure-metabolism coupling control, increasing the pressure of the fermentation tank at a specific rate, and adding coenzyme Q10; the quorum sensing inhibitory peptide containing a specific core structure is synchronously released in the boosting process; after the pressure is stable, a real-time mapping relation between a microbial metabolism state and physical parameters is constructed in a thallus proliferation stage through a coupling mechanism of dissolved oxygen gradient control and quorum sensing signal monitoring, and the industrial problem that a traditional static threshold value cannot respond to pressure fluctuation of a fermentation tank to cause signal attenuation is solved.

The invention provides an angiotensin converting enzyme inhibitory peptide and application thereof. The amino acid sequence of the angiotensin converting enzyme inhibitory peptide is PVKP. The ACE inhibitory activity of the inhibitory peptide PVKP provided by the invention is obviously improved compared with that of the original inhibitory peptide LYPVK, and the IC50 value is reduced from 10.6 mu g / mL to 9.6 mu g / mL; the digestive enzyme stability of the inhibitory peptide PVKP provided by the invention and the digestive enzyme stability of the original inhibitory peptide LYPVK under the same concentration are improved. Molecular simulation docking verifies that the interaction force between the ACE inhibitory peptide PVKP and ACE is enhanced compared with that of an original inhibitory peptide LYPVK, and the interaction force between alkyl groups and the interaction between the alkyl groups and Zn < 2 + > of an ACE active center are increased. The intracellular antihypertensive activity of the ACE inhibitory peptide PVKP is further verified through cell experiments, and compared with the original inhibitory peptide LYPVK, the intracellular NO release content is increased, and the ET-1 content is reduced. The ACE inhibitory peptide PVKP is small in molecular weight and good in water solubility, and application of the ACE inhibitory peptide PVKP in development of products with the blood pressure lowering effect is facilitated.

The invention relates to the technical field of biochemistry, in particular to a specific affinity enrichment and preparation method of a torreya grandis protease hydrolysate ACE inhibitory peptide, which comprises the following steps: step 1, preparing and characterizing magnetic aminated Fe3O4 nanoparticles; step 2, preparing and characterizing the immobilized trypsin; 3, preparing and characterizing the immobilized ACE, and optimizing reaction conditions; step 4, determining the activity of the immobilized ACE; step 5, then preparing and specifically enriching the Chinese torreya antihypertensive peptide; step 6, then, carrying out molecular docking through AutoDock Vina; according to the method, genipin is adopted as a cross-linking agent, magnetic Fe3O4 nanoparticles with the ACE inhibitory peptide specific affinity recognition function are constructed, specific enrichment of the ACE inhibitory peptide from the torreya grandis cake protein hydrolysate is achieved, and specific separation is achieved.

The embodiment of the invention discloses a goat milk-derived ACE (angiotensin converting enzyme) inhibitory peptide monomer as well as a preparation method and application thereof, relates to the technical field of protein separation, and aims to improve a preparation source and preparation efficiency of a food-borne protein-derived ACE inhibitory peptide. The preparation method comprises the following steps: dissolving sheep milk casein in water, and carrying out heating treatment, enzymolysis treatment and freeze drying treatment to obtain a sheep milk source zymolyte; preparing the goat milk source zymolyte into a solution with the concentration of 145mg / mL-155mg / mL, passing through a 0.45 mu m filter membrane, and carrying out primary separation by adopting preparative high performance liquid chromatography to obtain a product Y; preparing the Y product into a solution with the concentration of 20 mg / mL-30 mg / mL, passing through a 0.45 mu m filter membrane, and carrying out secondary separation by adopting preparative high performance liquid chromatography to obtain a Y-X product; preparing the Y-X product into a solution with the concentration of 5mg / mL-15mg / mL, passing through a 0.45 mu m filter membrane, and carrying out re-separation preparation by using a C18 hydrophilic preparative column and preparative high performance liquid chromatography to obtain a product M; the product M is prepared into a solution with the concentration of 5 mg / mL-15 mg / mL, the solution passes through a 0.22-micron filter membrane, after re-separation preparation is conducted through RP-HPLC, rotary evaporation concentration treatment and freeze drying treatment are conducted, and a product F is obtained.

The invention provides a dietary fiber composition for reducing weight and regulating intestinal flora in a targeted manner as well as a preparation method and application of the dietary fiber composition. The composition is prepared from the following raw materials in parts by weight: 20 to 30 parts of inulin, 15 to 25 parts of germinated brown rice powder, 10 to 20 parts of pectin, 8 to 15 parts of guar gum, 12 to 20 parts of beta-glucan, 15 to 25 parts of konjac glucomannan, 10 to 20 parts of multi-element dietary fiber powder, 5 to 10 parts of compound probiotic powder, 3 to 8 parts of bitter gourd polypeptide, 3 to 8 parts of folium mori extract and 2 to 5 parts of protein hydrolysate. Multi-element dietary fiber powder, prebiotics and the like are combined, endogenous GLP-1 is stimulated, the characteristics that the protein nutrient is not easily absorbed by people, but can be utilized by intestinal flora to generate SCFA and stimulate GLP-1 secreted by L cells at the far end of the intestinal tract are fully exerted, the requirements of most people for weight losing and weight control can be met, the protein nutrient can serve as a source of DDP-IV inhibitory peptide, decomposition of the endogenous GLP-1 is slowed down, and the weight losing and weight control effects are achieved. A more lasting effect of endogenous GLP-1 is ensured; a hot extrusion embedding technology and an airflow cutting technology are adopted, so that more nutrient substances can be effectively reserved in intestinal tracts at the same time, and nutrients are provided for flora.

The invention discloses lactobacillus helveticus for producing DPP-IV (dipeptidyl peptidase-IV) inhibitory peptide and hypoglycemic goat milk and a preparation method thereof, goat milk is used as a raw material, and lactobacillus helveticus KD12 for producing the DPP-IV inhibitory peptide is used for fermenting the goat milk to obtain hypoglycemic lactobacillus fermented goat milk containing the DPP-IV inhibitory peptide. And drying the fermented goat milk to obtain the hypoglycemic lactic acid bacteria fermented goat milk powder containing the DPP-IV inhibitory peptide. Lactic acid bacteria of the hypoglycemic lactic acid bacteria fermented goat milk are inactivated to obtain hypoglycemic post-prebiotics goat milk, and the hypoglycemic post-prebiotics goat milk powder is obtained after the hypoglycemic post-prebiotics goat milk is concentrated and dried. The hypoglycemic goat dairy product provided by the invention has a relatively high DPP-IV inhibition rate, and can be used for inhibiting DPP-IV activity after consumers eat the hypoglycemic goat dairy product so as to reduce blood sugar and reduce the dosage of hypoglycemic drugs, so that side effects caused by the hypoglycemic drugs are reduced.

The invention belongs to the field of functional polypeptides, and discloses an elastinase inhibitory peptide and a preparation method thereof. The elastase inhibitory peptide comprises nine types of oligopeptides from crayfish, and the amino acid sequences of the oligopeptides are respectively GASGPAGPRGPVGVA, IDGF, GAAGPAGPRGPAGPA, SGPAGPA, GPAGPRGPVGVA, SGPAGPRGPVGVA, VGPQGASGPLGPA and IGPA. The invention further discloses a preparation method of the elastase inhibitory peptide. The elastinase inhibitory peptide disclosed by the invention can be chemically synthesized and can also be directionally prepared from fish gelatin enzymolysis products. The elastase inhibitory peptide disclosed by the invention has good binding force with elastase and has remarkable elastase inhibitory activity.