Method for preparing ACE inhibitory peptides through bean pulp enzymolysis

A technology of enzymatically hydrolyzing soybean meal and inhibiting peptides, which is applied in the food field, can solve the problems of lack of cheap and effective enzyme preparations, affect the purification effect, and cannot effectively solve the bitter taste of enzymolyzed products, so as to achieve convenient storage and consumption, a wide range of applications, and convenient The effect of machining operations

Inactive Publication Date: 2016-04-27
TIANJIN MODERN VOCATIONAL TECH COLLEGE
View PDF5 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, ACE inhibitory peptides have not been put into large-scale production and application. The main reasons are: the lack of cheap, effective and easy-to-manufacture enzyme preparations, the inability to effectively control the molecular

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A method for enzymatically hydrolyzing soybean meal to prepare ACE inhibitory peptides, comprising the steps of:

[0038] (1) Soybean meal pretreatment

[0039] The high-temperature soybean meal is crushed with a pulverizer and passed through a 60-mesh sieve. The obtained soybean meal powder is mixed with water at a mass ratio of 1:5, stirred evenly, and the pH value is adjusted to 9. Ultrasonic is carried out at a power of 300W, a temperature of 40°C, and a time of 15 minutes. Obtain soybean meal mixture;

[0040] (2) Enzymolysis

[0041] Adjust the pH value of the soybean meal mixture to 7.2, add a compound enzyme preparation of 4% of the mass of the soybean meal mixture, mix evenly, and hydrolyze the hydrolyzate for 6 hours at a temperature of 40°C, then boil the hydrolyzate in a boiling water bath for 10 minutes, and cool to room temperature. Obtain mixed liquor; Wherein, composite enzyme preparation comprises the raw material that is made of alkaline protease, tr...

Embodiment 2

[0047] A method for enzymatically hydrolyzing soybean meal to prepare ACE inhibitory peptides, comprising the steps of:

[0048] (1) Soybean meal pretreatment

[0049] The high-temperature soybean meal is crushed with a grinder and passed through a 60-mesh sieve. The obtained soybean meal powder is mixed with water at a mass ratio of 1:4, stirred evenly, and the pH value is adjusted to 8. Ultrasonic is carried out, with ultrasonic power of 400W, temperature of 40°C, and time of 10 minutes. Obtain soybean meal mixture;

[0050] (2) Enzymolysis

[0051] Adjust the pH value of the soybean meal mixture to 8, add a compound enzyme preparation of 5% of the mass of the soybean meal mixture, mix evenly, and hydrolyze the hydrolyzate for 5 hours at a temperature of 50°C, then boil the hydrolyzate in a boiling water bath for 10 minutes, and cool to room temperature. Obtain mixed liquor; Wherein, composite enzyme preparation comprises the raw material that is made of alkaline protease,...

Embodiment 3

[0057] A method for enzymatically hydrolyzing soybean meal to prepare ACE inhibitory peptides, comprising the steps of:

[0058] (1) Soybean meal pretreatment

[0059] The high-temperature soybean meal is crushed with a pulverizer and passed through a 60-mesh sieve. The obtained soybean meal powder is mixed with water at a mass ratio of 1:6, stirred evenly, and the pH value is adjusted to 10. Ultrasonic is carried out at a power of 150W, a temperature of 50°C, and a time of 20 minutes. Obtain soybean meal mixture;

[0060] (2) Enzymolysis

[0061] Adjust the pH value of the soybean meal mixture to 9, add a compound enzyme preparation of 3% of the mass of the soybean meal mixture, mix well, and hydrolyze the hydrolyzate for 8 hours at a temperature of 35°C, then boil the hydrolyzate in a boiling water bath for 10 minutes, and cool to room temperature. Obtain mixed liquor; Wherein, composite enzyme preparation comprises the raw material that is made of alkaline protease, tryps...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptides through bean pulp enzymolysis. The method comprises the following steps that (1) high-temperature bean pulp is crushed and sieved, obtained bean pulp powder and water are mixed according to a mass ratio of 1:(4-6), the pH value is regulated to 8 to 10, and a bean pulp mixture is obtained; (2) the pH value of the bean pulp mixture is regulated to 7 to 9, a complex enzyme preparation is added, the mixture is uniformly mixed, hydrolysis is carried out for 5 to 8 hours at the temperature being 35 to 50 DEG C, after the hydrolysis is completed, hydrolysis liquid is boiled in a boiling water bath for enzyme deactivation, and mixed liquid is obtained; (3) the pH value of the mixed liquid is regulated to 6.5 to 7.5, supernatant is taken, ultrafiltration treatment is performed through an ultrafiltration membrane, and filtered liquid is obtained; and (4) the filtered liquid is subjected to vacuum freezing drying, and the ACE inhibitory peptide powder is obtained. The method for preparing ACE inhibitory peptides through bean pulp enzymolysis has the advantages that the process is simple and is easy to implement; in the whole process, the reaction conditions are mild; the compounded complex enzyme preparation is used; and the industrial processing operation is convenient.

Description

technical field [0001] The invention belongs to the technical field of food, and in particular relates to a method for preparing ACE inhibitory peptides by enzymatically hydrolyzing soybean meal. Background technique [0002] ACE inhibitory peptides, namely angiotensin-converting enzyme inhibitory peptides (ACEIP, AngiotensinConvertingEnzymeInhibitoryPeptides), are some polypeptide substances that inhibit angiotensin-converting enzyme (ACE) activity. The amino acid sequences and peptide chain lengths of these polypeptides are different, but all It has a similar function, that is, it has the function of lowering blood pressure, so it is also called blood pressure lowering peptide. In 1981, the first synthetic orally effective peptide ACE inhibitor, captopril, came out. So far, many chemically synthesized ACE inhibitors have been used clinically. However, the action time of this kind of drugs is not long, and blood pressure will rebound after drug withdrawal; excreted by the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 刘皓侯婷王立晖揣玉多李达刘鹏杨庆伟吕春晖李晓阳魏玮傅维范兆军
Owner TIANJIN MODERN VOCATIONAL TECH COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap