31 results about "Captopril" patented technology

The invention aims to provide a preparation method and application of a green fluorescent carbon dot for detecting captopril, and belongs to the technical field of fluorescent carbon dots, diphenylamino-4-benzaldehyde and o-phenylenediamine are weighed and dissolved in ethanol, and ultrasonic treatment is performed to obtain a uniformly mixed solution; transferring the solution into a polytetrafluoroethylene reaction kettle, reacting for 2-8 hours at the temperature of 150-220 DEG C, standing and cooling to room temperature after the reaction is stopped, centrifuging to remove insoluble substances, taking supernate, and dialyzing for at least three days in a glass container through a dialysis bag of 500-1000Da to obtain a pure carbon dot aqueous solution; and freeze-drying the carbon dot aqueous solution to obtain a carbon dot solid. The prepared green fluorescent carbon dots can be used for detecting captopril, and are good in selectivity and high in sensitivity. Compared with other carbon dot-based fluorescence sensing methods, the method provided by the invention can be used for directly detecting captopril.

The invention relates to the technical field of catalyst preparation, in particular to a supported TBD-IPTS / SBA-15 catalyst and a preparation method and application thereof.The preparation method comprises the following steps that an SBA-15 carrier is treated with acid liquor, washed to be neutral and then dried, and an activated carrier SBA-15-OH is obtained; the preparation method comprises the following steps: dispersing SBA-15-OH in anhydrous toluene under the protection of nitrogen, adding IPTS (isopropyl-beta-d-thiosulfate) for reflux reaction, and washing and drying after the reaction is finished to obtain IPTS / SBA-15; iPTS / SBA-15 is dispersed in anhydrous dichloromethane, a TBD solution is added for a reaction, washing and drying are performed after the reaction is finished, and a supported TBD-IPTS / SBA-15 catalyst is obtained; the problems that in existing captopril intermediate synthesis, a by-product L-MAT is difficult to utilize, a traditional homogeneous TBD catalyst is difficult to recycle and reuse, and in an existing loading technology, active components are poor in stability and low in catalytic efficiency are solved.

The invention relates to an ROS (reactive oxygen species) response type nano prodrug based on dynamic differentiation of monocytes as well as a preparation method and application of the ROS response type nano prodrug. According to the preparation method, citral and captopril react to form a captopril-citral prodrug molecule (Citopril) through a thioketal bond, and then the prodrug molecule and polyethylene glycol lipid are self-assembled in a water-containing solvent, so that the ROS response type nano prodrug (Citopp-NM) capable of being efficiently taken in by blood monocytes is prepared. According to the nano prodrug, dynamic differentiation of mononuclear cells is fully utilized, a nano drug delivery strategy of the mononuclear cells and the ROS responsive activation characteristic of the nano prodrug are combined, more efficient enrichment of the nano prodrug in AS plaques is achieved, activation of the nano prodrug is controlled through differentiation of the mononuclear cells, and the nano prodrug has a good application prospect. Therefore, distribution and activation of drugs in normal tissues can be reduced, the drug effect is improved, and toxic and side effects of the drugs are reduced.

The present application relates to a kind of monodisperse core-shell type hybrid silica microspheres containing vinyl shell and its preparation and application. Specifically, using "two-phase method" strategy, monodisperse solid silica microspheres are used as matrix, vinyl triethoxysilane and tetraethyl orthosilicate are used as raw materials, lauryl amide propyl betaine and sodium subtiliporin are used as double-template pore-forming agent, under the catalysis of urea, sol-gel reaction occurs, a layer of porous hybrid shell containing vinyl functional group is constructed on the surface of solid silica microspheres, and core-shell type silica hybrid microspheres are formed. Because the shell contains a large number of vinyl functional groups, after hydrophilic modification with captopril, it is used as the adsorbent of hydrophilic interaction chromatography, and is applied to the selective enrichment of biological samples. The core-shell type silica hybrid microspheres prepared in the present application have uniform morphology, good monodispersity, and rich vinyl functional groups on the surface, which are easy to modify and apply subsequently.

The utility model is applicable to the technical field of captopril sheet production, and provides a raw material crushing and screening device which comprises a processing box for crushing captopril sheet raw materials, and a crushing chamber, a recycling chamber and a screening chamber are arranged in the processing box; the first crushing roller is rotationally mounted in the crushing chamber in a meshed manner and is used for crushing captopril sheet raw materials; the second crushing roller is rotationally mounted in the recovery chamber in a meshed manner and is used for crushing the screened topril sheet raw materials; the driving motors are respectively fixed on the outer side of the crushing chamber and the outer side of the recycling chamber and are used for driving the first crushing roller and the second crushing roller; and the driving motors are fixedly connected with shaft rods corresponding to the first crushing roller and the second crushing roller. According to the raw material crushing and screening device provided by the scheme, by optimizing the structural design and function configuration, further effective treatment of screened raw materials is achieved, and the raw material utilization rate, the product quality and the production efficiency are improved.

This invention discloses an octapeptide derived from soybean protein with the function of lowering blood pressure, blood sugar, and cholesterol, as well as its preparation and application, belonging to the field of small molecule peptide technology. The octapeptide sequence is YEGNWGPL, which can be obtained artificially or by targeted enzymatic hydrolysis of soybean protein isolate. Functional verification in a hyperlipidemia model shows that the octapeptide YEGNWGPL has the function of lowering blood lipids; functional verification in a hyperglycemia model shows that the octapeptide YEGNWGPL has the function of lowering blood sugar, with a significantly better effect than the drug metformin; functional verification in a hypertension model shows that the octapeptide YEGNWGPL has the function of lowering blood pressure, with a significantly better effect than the antihypertensive drug captopril; moreover, food-derived bioactive peptides have high biosafety, therefore, they can be applied to the development of related products for lowering blood pressure, blood sugar, and cholesterol or assisting in lowering blood pressure, blood sugar, and cholesterol, with good market prospects and application potential.

The present invention provides a twelve peptide with ACE inhibitory activity and a screening method and application thereof, belonging to the field of polypeptide technology. The present invention isolates a twelve peptide from wheat fermentation broth, and through sequencing analysis, the amino acid sequence of the twelve peptide is Pro-Tyr-Ser-Ala-Gly-Gln-Leu-Ser-Ala-Cys-Leu-GLn (PYSAGQLSACLQ). The twelve peptide of the present invention has the ability to inhibit ACE enzyme activity in vitro, and animal experiments also show that the twelve peptide has an effect close to that of the mainstream antihypertensive drug captopril, and has a high application value.

The invention discloses cordyceps sobolifera polysaccharide with an effect of reducing blood pressure and a microbial fermentation preparation method of the cordyceps sobolifera polysaccharide, and belongs to the field of preparation and application of fungal polysaccharide extracts. The cordyceps sobolifera polysaccharide is prepared by adopting a microbial fermentation method, cordyceps sobolifera coremium is used as a fermentation substrate, plant lactobacillus is used as a fermentation strain, liquid fermentation extraction is performed to obtain the cordyceps sobolifera polysaccharide CCP-Lp, the monosaccharide composition and the molar ratio of mannose to glucose to galactose are 1: 0.36: 0.62, and the relative molecular mass is 1.32 * 10 < 5 > Da. Efficacy experiments show that CCP-Lp can significantly inhibit abnormal increase of systolic pressure and diastolic pressure of spontaneously hypertensive rats and regulate the key factor level of a renin-angiotensin-aldosterone system, and the antihypertensive effect of CCP-Lp can be comparable with that of a positive drug captopril. The cordyceps sobolifera polysaccharide CCP-Lp is clear in composition and remarkable in effect, and has wide development and application prospects in the aspect of blood pressure regulation and control.

The application discloses a cordyceps cicadae and cordyceps sinensis polysaccharide with blood pressure lowering efficacy and a microbial fermentation preparation method thereof, and belongs to the field of fungal polysaccharide extract preparation and application. The cordyceps cicadae and cordyceps sinensis polysaccharide is prepared by a microbial fermentation method, a cordyceps cicadae and cordyceps sinensis spore bundle is used as a fermentation matrix, and bacillus lactis is used as a fermentation strain; after liquid fermentation extraction, the cordyceps cicadae and cordyceps sinensis polysaccharide CCP-Lp is obtained, the monosaccharide composition and molar ratio of which are as follows: mannose: glucose: galactose = 1: 0.36: 0.62, and the relative molecular mass is 1.32*10 5 Da. Efficacy experiments show that the CCP-Lp can significantly inhibit abnormal increase of systolic pressure and diastolic pressure of spontaneous hypertension rats, and regulate levels of key factors of a renin-angiotensin-aldosterone system; the antihypertensive effect of the CCP-Lp is comparable to that of a positive drug, captopril. The cordyceps cicadae and cordyceps sinensis polysaccharide CCP-Lp has clear composition and significant efficacy, and has a broad development and application prospect in regulating blood pressure.

The invention discloses an application of ilicin A in preparation of a medicine for preventing and treating ischemic heart disease, and belongs to the technical field of ilicin A. An experiment for improving heart functions of a mouse I / R model by the ilicin A is carried out, and male C57BL / 6 mice (with the age of 6-8 weeks; according to the method, 70 mice (the weight is 20 + / -2g) are bred in a constant-temperature and constant-humidity environment, the illumination / darkness period is 12 hours and lasts for one week, food and water are obtained without limitation, all male mice are randomly grouped as follows: a false operation group (n = 10), an I / R group (n = 15), an ilicin A group (30 and 60 mg / kg) and a captopril group (20 mg / kg), the mice in the ilicin A group and the captopril group are subjected to intragastric administration every day five days before the model is established, and the mice in the captopril group are subjected to intragastric administration every day. And the isopyknic contrast solution is applied to the false operation group and the I / R group.

This invention discloses a soybean protein small molecule peptide with the effect of lowering blood pressure, blood sugar, and cholesterol, as well as its preparation and application, belonging to the field of small molecule peptide technology. The soybean protein small molecule peptide is obtained by targeted enzymatic hydrolysis of soybean protein isolate, containing the bioactive peptide PLEGFPF with the effect of lowering blood pressure, blood sugar, and cholesterol. Functional verification shows that the bioactive peptide PLEGFPF and the soybean protein small molecule peptide containing this peptide have the function of lowering blood lipids; it has the function of lowering blood sugar, with a lower effective concentration and better effect compared with the positive drug metformin; it has the function of lowering blood pressure, with a lower effective concentration and better effect compared with the positive drug captopril. Food-derived bioactive peptides have high biosafety; therefore, they can be applied to the development of related products for lowering or assisting in lowering blood pressure, blood sugar, and cholesterol, with good market prospects and application potential.

The application provides a trifunctional tetravalent platinum compound of captopril-aspirin ligand and a reference drug of a bifunctional tetravalent platinum compound of captopril; and develops a nanomedicine Tf-GEVs@Pt(IV) of the aspirin-captopril trifunctional tetravalent platinum compound loaded in grapefruit extracellular vesicles (GEVs). The Tf-GEVs@Pt(IV) is prepared from the trifunctional tetravalent platinum compound as an active ingredient, the carrier grapefruit extracellular vesicles (GEVs), the carrier DSPE-PEG and the transferrin modified targeting carrier DSPE-PEG-Tf. The Tf-GEVs@Pt(IV) can release the active component trifunctional tetravalent platinum compound in vivo. In addition to causing DNA damage, the trifunctional tetravalent platinum compound as the active component of the nanomedicine can effectively regulate inflammatory, fibrotic and immunosuppressive TME. The nanomedicine has a significant anti-tumor ability and has excellent therapeutic effect on metastatic malignant tumors, and is expected to provide a new candidate drug for clinical treatment of tumors and provide a new direction for research and development of new platinum drugs and anti-metastatic malignant tumor drugs.

The invention relates to a hybridoma cell strain secreting an anti-captopril monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of drug safety immunoassay. The hybridoma cell strain secreting the anti-monoclonal antibody is preserved in China General Microbiological Culture Collection Center (CGMCC) on the year, the preservation number is CGMCC No.46502, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the hybridoma cell strain is classified and named as a monoclonal cell strain. The captopril monoclonal antibody cell strain obtained by the invention can be used for drug quality detection and captopril residue detection in a biological sample, has better detection sensitivity (IC50 value is 3.433 ng / mL) and specificity (the cross-over rate for related structural analogues is less than 1%) to captopril, and can realize detection of captopril in drugs and biological samples; and an efficient immunodetection tool is provided for clinical medication detection and drug quality control, the clinical medication safety and effectiveness are guaranteed in an assisted manner, and the kit has a definite practical application value.

The invention provides application of 2-methoxyestradiol in preparation of products for preventing and treating heart failure, and belongs to the technical field of medicines. The invention provides the effect of 2-methoxyestradiol (2ME) for preventing and treating heart failure for the first time. According to the invention, an isoproterenol induced myocardial fibroblast proliferation and migration model and an in-vivo heart failure mouse model are constructed in vitro; according to the present invention, the cardiac muscle fibroblast migration rate, the cardiac function, the serum heart failure marker level, the cardiac muscle tissue collagen deposition key protein, the pathological analysis and the heart electrophysiological signal change on the heart failure mouse are detected, the anti-heart failure effect of the 2ME is researched, and the results show that the 2ME has significant treatment effect on the heart failure disease, and is superior to the positive control drug captopril;

This invention discloses a casein-derived small molecule peptide with antihypertensive effect and its preparation method, belonging to the field of bioactive peptide technology. The preparation method is as follows: S1, mix casein with water and stir until homogeneous; S2, add a complex protease for enzymatic hydrolysis, then add bromelain for further enzymatic hydrolysis to inactivate the enzyme; S3, centrifuge the mixture; S4, heat the separated mixture, add activated carbon for adsorption, then add diatomaceous earth, stir until homogeneous, and filter; S5, further filter through a membrane; S6, concentrate the mixture, sterilize, and dry to obtain the casein-derived small molecule peptide. The casein-derived small molecule peptide of this invention can effectively lower blood pressure by inhibiting ACE activity and blocking the production of angiotensin II. This small molecule peptide significantly increases blood flow activity and angiotensin II content in a hypertensive model zebrafish, exhibiting a dose-dependent effect, achieving the same effect as the antihypertensive drug captopril, and with an efficacy concentration that is half that of the positive control drug.

The invention relates to the field of biological medicine, in particular to a three-component platinum (IV) complex containing captopril (CTP) and valproic acid (VPA), a preparation method of the three-component platinum (IV) complex and application of the three-component platinum (IV) complex in preparation of anti-tumor proliferation and anti-metastasis drugs. The anti-tumor proliferation and anti-metastasis effects of the target compound are tested through in-vivo and in-vitro testing means, and the anti-tumor mechanism of the medicine is researched. Results prove that the compound has a remarkable anti-tumor capability, particularly has an excellent treatment effect on metastatic malignant tumors, is expected to provide a new candidate drug for clinical treatment of tumors, and provides a new direction for research and development of novel platinum drugs and anti-tumor metastasis drugs.

The invention relates to a preparation method of a sulfydryl gold nano-cluster with captopril providing a ligand and application of the sulfydryl gold nano-cluster in electrochemical luminescence detection, and belongs to the technical field of electrochemical luminescence detection. The invention develops and verifies that the gold nanocluster of the sulfydryl ligand is used as an electrochemical luminescence material, has good water solubility, and can react with a co-reactant of tripropylamine (TPrA) and cephalosporin to achieve an efficient electrochemical luminescence behavior. On one hand, the problems that a traditional electrochemical luminescence material is poor in water solubility and a reaction tank is not easy to clean are solved, and on the other hand, an electrochemical luminescence detection technology is applied to trace detection of ceftriaxone sodium for the first time. The ceftriaxone sodium is detected according to the acceleration effect of ceftriaxone sodium with different concentrations on electron transfer in the electrochemical luminescence process and different response intensities of electrochemical luminescence signals.

The invention discloses Mopan persimmon polypolyphenol series sulfydryl flavanol derivatives as well as a preparation method and application thereof, belongs to the technical field of plant polypolyphenol component degradation and degradation product separation and preparation and the technical field of biological medicines, and particularly relates to preparation of 16 series sulfydryl flavanol derivatives and application of the 16 series sulfydryl flavanol derivatives in the anti-inflammatory aspect. Based on an oriented transformation strategy of a functionalized nucleophilic reagent, a reaction reagent with a characteristic functional group is introduced, and a structure-activity oriented degradation reaction system is constructed. The method comprises the following steps: by taking Mopan persimmon fruit poly-polyphenol extract as a raw material and methanol as a solvent under an acid catalysis condition, degrading a persimmon fruit poly-polyphenol extracting solution into extension units containing carbocations, and respectively combining the extension units with captopril, L-cysteine, N-acetylcysteine and glutathione to generate a series of sulfydryl flavanol derivatives. The sulfydryl flavanol derivative disclosed by the invention has obvious anti-inflammatory activity and can be used for preparing anti-inflammatory drugs.

The invention discloses a detection method for determining isomers in captopril. The isomers comprise a chiral isomer 1, a chiral isomer 2 and a chiral isomer 3. According to the detection method, a high performance liquid chromatograph is used, a mobile phase is a mixed solution, and isocratic elution is adopted; the invention provides a detection method for simultaneously determining three chiral isomers in captopril by adopting a high performance liquid chromatograph according to the structural characteristics of a product and the properties of a compound. The isocratic chromatographic conditions are adopted, each chiral isomer and captopril can be effectively separated, the detection sensitivity and accuracy of each component are further improved by optimizing the conditions, the limit requirements of each impurity are met, and the detection cost and the operation difficulty are greatly reduced.

The invention provides a trifunctional tetravalent platinum compound of a captopril-aspirin ligand and a reference drug captopril difunctional tetravalent platinum compound of the trifunctional tetravalent platinum compound. The invention also develops a nano-drug Tf-GEVs (at) Pt (IV) of an aspirin-captopril three-function tetravalent platinum compound entrapped by grapefruit extracellular vesicles (GEVs). The Tf-GEVs (at) Pt (IV) is prepared from a trifunctional tetravalent platinum compound serving as an active component, grapefruit extracellular vesicles (GEVs) serving as a carrier, DSPE-PEG serving as a carrier, and a transferrin modified targeting carrier DSPE-PEG-Tf. The Tf-GEVs (at) Pt (IV) can release an active component, namely a trifunctional tetravalent platinum compound in vivo. The trifunctional tetravalent platinum compound as a nano-drug active component can cause DNA damage, and can also effectively regulate inflammatory, fibrosis and immunosuppressive TME. The nano-drug is remarkable in anti-tumor capacity, has an excellent treatment effect on metastatic malignant tumors, is expected to provide a new candidate drug for clinical treatment of tumors, and provides a new direction for research and development of novel platinum drugs and anti-metastatic malignant tumor drugs.

The utility model is applicable to the technical field of tablet manufacturing, and provides a rotary tablet press which comprises a rotary tablet press body mounted at the top of a base and used for extruding captopril tablets, the material guide plate is fixed on the rotary tablet press body, and the side section of the material guide plate is U-shaped; the collecting box is arranged on one side of the rotary tablet press body and is used for collecting captopril tablets; the collecting box is arranged in the material collecting box and used for isolating the complete captopril sheets, and a plurality of through holes are formed in the collecting box; and the screening assembly is mounted at the top of the base and is positioned on one side of the rotary tablet press body. The rotary tablet press provided by the scheme can be used for directly removing impurities from extruded tablets, so that the purity of the tablets is increased, and meanwhile, the production safety is relatively high.

This application discloses a method for detecting skin-whitening ingredients. Specifically, it provides a method for detecting skin-whitening ingredients, including carbimazole, 5-N-propyluracil, or peptide-amidazoline in cosmetics, filling the gap in existing technologies that lack methods for detecting these ingredients in cosmetics. This application also provides a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) method for detecting nine substances—methimazole, carbimazole, thiouracil, methylthiouracil, 5-N-propyluracil, propylthiouracil, captopril, ethionamide, and peptide-amidazoline—in a sample. This method is simple to operate, rapid, highly specific, and has high resolution. It can be used as a detection method for the above nine ingredients in cosmetics, food, pharmaceuticals, and health products, providing technical support for the analysis of prohibited substances and other substances in related categories in cosmetics.

The invention discloses a dual-load and dual-target nano material for treating acute kidney injury as well as a preparation method and application of the dual-load and dual-target nano material. The preparation method comprises the following steps: dropwise adding an organic solvent solution of triptolide and PLGA-PEG into an aqueous solution of Poloxamer 188, stirring to volatilize the organic solvent, and centrifuging to obtain TP (at) MNPs; the preparation method comprises the following steps: activating carboxyl of captopril, reacting with NH2-PEG-NH2, reacting with carboxyl-activated GFLG polypeptide, reacting with carboxyl-activated Nephrin antibody, and finally reacting with carboxyl-activated TP-coated MNPs, so as to obtain the double-load and double-target nano material. The dual-load and dual-targeting nano material has the advantages of graded release of drugs, protection of loaded drugs, dual long-range targeting of renal tubules and glomerulus and the like; tests on puromycin amino nucleoside model mice and ischemia reperfusion injury model mice prove that the traditional Chinese medicine composition has a good treatment effect on acute kidney injury.

The invention relates to a hybridoma cell strain secreting an anti-captopril monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of drug safety immunoassay. The hybridoma cell strain secreting the anti-monoclonal antibody is preserved in China General Microbiological Culture Collection Center (CGMCC) on the year, the preservation number is CGMCC No.46502, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the hybridoma cell strain is classified and named as a monoclonal cell strain. The captopril monoclonal antibody cell strain obtained by the invention can be used for drug quality detection and captopril residue detection in a biological sample, has better detection sensitivity (IC50 value is 3.433 ng / mL) and specificity (the cross-over rate for related structural analogues is less than 1%) to captopril, and can realize detection of captopril in drugs and biological samples; and an efficient immunodetection tool is provided for clinical medication detection and drug quality control, the clinical medication safety and effectiveness are guaranteed in an assisted manner, and the kit has a definite practical application value.

The invention belongs to the technical field of biological medicines, and particularly relates to application of limonin in preparation of a medicine for improving renal fibrosis. The limonin used in the invention is also named evodia lactone, the CAS number of the limonin is 1180-71-8, the molecular formula of the limonin is C26H30O8, and the structure of the limonin compound is as shown in the formula I. The limonin compound can be applied to the field of pharmaceutical chemistry. According to the application, a unilateral ureteral obstruction model is constructed, a positive drug captopril is taken as a control group, and the application of limonin in improvement of renal fibrosis caused by acute kidney injury is verified. Results of embodiments show that limonin not only has low cytotoxicity and high safety, but also has anti-inflammatory and anti-fibrosis effects, and can inhibit proliferation and migration of renal fibroblasts and improve mitochondrial functions of renal epithelial cells.

The invention discloses health wine with double-effect synergy of reducing blood pressure, strengthening waist and tonifying kidney and a preparation method thereof. The health wine is prepared from 20-35 parts of eucommia ulmoides leaves, 15-30 parts of astragalus membranaceus, 10-25 parts of Chinese dates, 8-20 parts of herba epimedii and 5-15 parts of semen cuscutae through a gradient temperature control extraction process. Through CO2 supercritical deastringency and two-stage enzymolysis extraction, the extraction rate of key components such as chlorogenic acid and astragaloside is increased by 28-45%; a chitosan-sodium alginate nano-carrier is introduced to load sodium selenite, so that targeted delivery of active ingredients is realized, and the bioavailability is improved by 2.1 times compared with that of a traditional preparation; a sustained-release system is constructed by adopting a beta-cyclodextrin inclusion technology, so that the efficacy duration time is prolonged. Animal experiments prove that the antihypertensive effect of the preparation is not statistically different from that of captopril, the kidney SOD activity can be improved by 65%, the serum testosterone level can be improved by 35%, the product has the advantages of being clear in effect, high in safety and stable in process, and the blank in the prior art is filled.

The present invention discloses a captopril-modified hollow nanosilica sphere and its preparation method and application, belonging to the technical field of nanomaterials. Among them, the preparation method of the captopril-modified hollow nanosilica sphere is as follows: using water as a solvent, F127 as a template agent, mesitylene as a micelle swelling agent, potassium sulfate as an auxiliary agent, and tetramethoxysilane and vinyltrimethoxysilane as silicon-based precursors, preparing vinyl-modified hollow nanosilica spheres through a sol-gel reaction; then, further modifying captopril on the hollow nanosilica spheres through a thiol-ene photoinitiated reaction. The beneficial effects of the present invention are as follows: The captopril-modified hollow nanosilica sphere is the first hydrophilic interaction chromatography stationary phase material prepared with captopril as a modifier monomer, having uniform particle size, good dispersibility, not easy to agglomerate, large specific surface area, and having good enrichment specificity and selectivity for N-glycopeptides, and can be used for the selective enrichment of N-glycopeptides in complex biological samples.

The present invention relates to a liquid chromatography stationary phase, characterized in that the drug molecule captopril is used to modify the silica gel surface, the bonded phase contains a carboxyl group, a thioether bond and an amide group, and the structural formula is as follows: wherein Silica Gel is silica gel, and n=0-10. The present invention also provides a preparation method of the above-mentioned liquid chromatography stationary phase, and captopril is modified to the silica gel surface by a thiol-olefin click reaction to prepare a novel weak cation exchange chromatography stationary phase. The separation material preparation method provided by the present invention is simple and efficient, and a variety of functional groups can provide different separation selectivities, which can be widely used in the separation and analysis of various compounds.

A model teaching aid for the mechanism of action of captopril includes a captopril module, an angiotensin-converting enzyme module, a body module, and a connecting device. The body module includes a blood vessel cross-section model, a heart cross-section model, an angiotensin I reference model, an angiotensin II reference model, a bradykinin reference model, an NO reference model, and a PGI2 reference model. The blood vessel cross-section model and the heart cross-section model are arranged on the connecting device. The angiotensin I reference model, angiotensin II reference model, and bradykinin reference model are arranged inside the blood vessel cross-section model and the heart cross-section model. The captopril module and the angiotensin-converting enzyme module are arranged inside the blood vessel cross-section model and the heart cross-section model. The model teaching aid of the present invention has a three-dimensional model structure and is highly intuitive, making it easier for students to deeply understand the mechanism of action of captopril, thereby facilitating the teaching of pharmacology courses.

The invention discloses a detection method of whitening components. The invention provides a method for detecting whitening components including carbinomizole, 5-N-propyluracil or peptidomidol in cosmetics, and the method makes up the defect that a method for detecting carbinomizole, 5-N-propyluracil or peptidomidol in cosmetics does not exist in the prior art. The invention further provides a high performance liquid chromatography-tandem mass spectrometry detection method for nine substances including methimazole, carbinomizole, thiouracil, methylthiouracil, 5-N-propyluracil, propyl thiouracil, captopril, ethionamide and peptidomidol in a to-be-detected sample. The detection method is simple to operate, rapid in analysis, high in specificity and high in separation degree. The invention further provides a high performance liquid chromatography-tandem mass spectrometry detection method for the nine substances including methimazole, carbinomizole, thiouracil, methylthiouracil, 5-N-propyluracil, propyl thiouracil, captopril, ethionamide and peptidomidol. The method can be used as a detection method for the nine components in cosmetics, food, medicines and health care products, and provides technical support for analysis of forbidden substances of the cosmetics and other substances of related categories.