30 results about "Theophylline" patented technology

The invention belongs to the technical field of medicine detection, and particularly relates to an ICPMS (Inductively Coupled Plasma Mass Spectrometry) detection method for element impurities in theophylline. The linear test solution, the blank solution and the theophylline test solution are subjected to ICPMS detection, the content of element impurities in the theophylline test solution is calculated according to an internal standard curve method, and the element impurities are one or more of cadmium, lead, arsenic, mercury, cobalt, vanadium, nickel, lithium, antimony or copper. The method is good in reproducibility and stability, high in accuracy and high in precision.

The invention discloses allosteric DNA ribozyme and application thereof. The nucleotide sequence of the allosteric DNA ribozyme is selected from SEQ ID NO.01 to SEQ ID NO.10. The invention further discloses a preparation method of the allosteric DNA ribozyme The allosteric DNA ribozyme provided by the invention adopts a DNA structure, and compared with RNA ribozyme, the allosteric DNA ribozyme has higher chemical stability, is not easily degraded by nuclease, can complete theophylline detection within 60 minutes, does not need complex pretreatment or expensive instruments, is simple and convenient to operate, is suitable for bedside detection and household monitoring scenes, and greatly improves the detection efficiency.

The present application provides a composition with lipid-lowering and weight-reducing functions, which has clear components and controllable quality. The composition comprises Pu'er tea extract, wherein the Pu'er tea extract contains 10-90% theabrownine by weight, preferably also contains theophylline, the weight ratio of the theabrownine and theophylline is 10:1-60:1, and contains 0-15% tea polyphenols by weight. The theophylline can be selected from exogenous theophylline or theophylline contained in the Pu'er tea extract. The composition with lipid-lowering and weight-reducing functions of the present application also comprises Poria cocos extract; the weight ratio of the Pu'er tea extract to the Poria cocos extract is 1:3-3:1 based on the weight of theabrownine contained in the Pu'er tea extract. The composition of the present application has the advantages of clear effective components, simple preparation process, controllable quality, and stable lipid-lowering and weight-reducing effects.

The invention relates to a theophylline immunodetection reagent as well as a preparation method and a detection method thereof, in particular to a theophylline chemiluminescence immunodetection reagent as well as a preparation method and a detection method thereof, and the theophylline chemiluminescence immunodetection reagent comprises an anti-theophylline specific antibody and an indicating reagent for detecting an anti-theophylline specific antibody-theophylline conjugate, the anti-theophylline specific antibody is obtained by immunizing animals with theophylline immunogen. The theophylline immunogen has the beneficial effects that the theophylline immunogen has strong specificity and high immunogenicity, and the prepared anti-theophylline specific antibody has strong specificity and high titer; the chemiluminescence immunoassay reagent containing the anti-theophylline specific antibody can conveniently, rapidly and accurately determine the theophylline content in a sample, can simultaneously determine a plurality of samples on a full-automatic chemiluminescence immunoassay instrument, realizes high-flux rapid determination of theophylline, and has the advantages of high accuracy, strong specificity, high sensitivity and high sensitivity. And the accuracy and the detection efficiency are greatly improved.

This invention belongs to the field of photoelectrochemical detection, specifically relating to a method for fabricating a photo-assisted all-solid-state zinc-air battery self-powered sensor based on a signal reversal strategy and its application in detecting theophylline. Using CuInS2 as the photocathode, CdS is loaded onto the photocathode using a split aptamer technique. Polished zinc foil is used as the anode, and PVA gel polymer is used as the solid electrolyte to construct a photo-assisted all-solid-state zinc-air battery self-powered sensor based on a signal reversal strategy. This invention achieves signal reversal by incorporating split aptamer technology, thereby significantly improving the detection sensitivity for antioxidants. The constructed photo-assisted all-solid-state zinc-air battery self-powered sensor based on a signal reversal strategy also has a wide detection range, low detection limit, and low detection cost.

The invention provides a method for rapidly detecting the content of theophylline in plasma, urine, medical sewage and functional beverages. According to the method, aiming at theophylline analytes in different samples, firstly, the samples are pretreated by adopting a liquid phase microextraction (LPME) technology, and then the pretreated theophylline is detected by adopting liquid phase-tandem mass spectrometry (LC-MS / MS). According to the method, an external standard method is adopted for quantification, the established method is good in linearity, the linear range is 0.01-10 mg / L, the correlation coefficient of theophylline is larger than 0.999, the detection limit is 0.2 microgram / L, the quantification limit is 0.7 microgram / L, the adding standard recovery rate is 86.7%-111.3%, and the relative standard deviation is smaller than 9%. The method is easy to operate and convenient to use, has the advantages of being good in accuracy, high in precision and sensitivity and the like, and provides a reliable method for rapid detection of theophylline in various actual scenes such as medical treatment, market supervision and environment monitoring.

The invention belongs to the technical field of molecular biology, and particularly discloses a theophylline aptamer and a virtual screening method and application thereof. The theophylline aptamer is selected from one or more of MUT9, MUT16, MUT67, MUT78 or MUT89. The invention further discloses a preparation method of the theophylline aptamer. The invention discloses a theophylline aptamer as well as a virtual screening method and application thereof, the theophylline aptamer is high in affinity and specificity, the virtual screening method remarkably improves the accuracy and efficiency of virtual screening, the theophylline aptamer can be quickly obtained at low cost, and a powerful calculation tool is provided for rational design and application of the aptamer.

The invention relates to a preparation method of a caffeine impurity C. The preparation method of the caffeine impurity C can effectively solve the problems of preparation of the caffeine impurity C, effective control of impurity content in caffeine and guarantee of coffee quality, and the preparation method comprises the following steps: mixing theophylline, a catalyst triethylene diamine, N, N-dimethylformamide and dimethyl carbonate, heating for reaction, distilling, adding water for recrystallization, and filtering to obtain the caffeine impurity C. Recrystallized jellyfish liquid is obtained; and adding a halogenated hydrocarbon solvent, standing until a solid is separated out between the water layer and the halogenated hydrocarbon solvent layer, filtering, and drying to obtain the product. The invention provides the caffeine impurity preparation method which is simple and easy to implement, mild in condition and low in energy consumption and pollution, so that the preparation vacancy of the impurity at present is filled up, powerful technical support is provided for quality research and quality control of caffeine, and the method has practical popularization and application values.

The invention discloses an N-aryl nuciferine derivative and a preparation method and application thereof.The chemical structure of the N-aryl nuciferine derivative is shown in the formula I. In the formula I, an aromatic ring A is selected from a benzene ring, a naphthalene ring, biphenyl or nitrogen heteroaromatic ring; r is selected from an electron-donating group and an electron-withdrawing group, or two ends of R are connected with an aromatic ring A to form a ring, the substitution number of the electron-donating group is 1-2, and the substitution number of the electron-withdrawing group is 1. The N-arylated nuciferine derivative disclosed by the invention has anti-lipase activity superior to that of a parent compound, can inhibit lipid accumulation in a cell differentiation process, and has further development potential.

The present invention relates to an anti-inflammatory extract of soil microorganisms or a fraction thereof, wherein the microorganisms comprise one or more selected from Chlorophyta, Xanthophyceae or Cyanobacteria. The invention further relates to compositions comprising two or more of Lyso-DGTS, p-Coumaric acid (ester-linked), oleamide, theophylline, or DGTS. The invention further relates to pharmaceutical compositions comprising the anti-inflammatory extract of soil microorganisms or a fraction thereof or a composition comprising two or more of Lyso-DGTS, p-Coumaric acid (ester-linked), oleamide, theophylline, or DGTS as an active ingredient. The invention further relates to an anti-inflammatory extract of soil microorganisms or a fraction thereof or a pharmaceutical composition comprising an anti-inflammatory extract of soil microorganisms or a fraction thereof or a composition comprising two or more of Lyso-DGTS, p-Coumaric acid (ester-linked), oleamide, theophylline, or DGTS as an active ingredient for use as a medicament, wherein the microorganisms comprise one or more selected from Chlorophyta, Xanthophyceae or Cyanobacteria. The invention further relates to compositions comprising an anti-inflammatory extract of soil microorganisms or a fraction thereof, wherein the microorganisms comprise one or more selected from Chlorophyta, Xanthophyceae or Cyanobacteria.

The invention discloses a method for simultaneously sensing and detecting two components of theophylline / caffeine based on a derived carbon-based material, the detection linear ranges of theophylline and caffeine are respectively 20-500 [mu] mol / L and 10-500 [mu] mol / L, the detection limits are respectively as low as 0.017 [mu] M and 0.021 [mu] M, and the sensitivities are respectively as high as 19.97 [mu] A / mM and 11.39 [mu] A / mM. Moreover, the sensor has the characteristics of high selectivity, good reproducibility, good stability and the like when being used for simultaneously detecting the theophylline and the caffeine, and is successfully used for simultaneously detecting the theophylline and the caffeine in the health-care food weight-reducing tea. It is worthy of mentioning that the nano material is easy and convenient to prepare and operate and short in consumed time, meanwhile, the nano material has the advantages of being low in raw material cost, wide and easy to obtain and the like, waste is turned into wealth, and the concept of sustainable development and green environmental protection is met.

The invention discloses a theophylline alkaloid salt, a pharmaceutical composition thereof, a preparation method of the theophylline alkaloid salt, and an application of the theophylline alkaloid salt, and belongs to the technical field of pharmaceutical chemistry. Natural alkaloids with definite pharmacological activity, such as stachydrine, betaine, trigonelline, sophocarpidine, choline or berberine, are selected as saline-forming bases, and a series of novel theophylline alkaloid salts are developed. The functional salt formation strategy not only fundamentally changes the traditional theophylline salt formation mode, but also realizes a leap-through breakthrough from single improvement of solubility to synergistic interaction and active attenuation. Through inherent gastric mucosa protection, heart function regulation and other effects of alkaloid, an active attenuation mechanism is constructed, the core toxic and side effects of gastrointestinal irritation, heart excitability and the like caused by theophylline are effectively reduced, the risk of introduction of exogenous sensitizers is avoided, and the bioavailability of theophylline is improved. The clinical problems of narrow therapeutic window and large toxic and side effects of traditional theophylline and salt drugs thereof are effectively solved.

A pharmaceutical composition in the form of a transdermal patch comprising aminophylline or theophylline for use in the treatment or prevention of a hypomyelinating or a demyelinating disease or condition or a lesion of the peripheral or central nervous system where demyelination occurs. The patch may comprise a matrix layer comprising a matrix polymer, an active ingredient and a permeation enhancer.

The invention discloses a preparation method of a theophylline controlled-release tablet and the theophylline controlled-release tablet. The preparation method comprises the following steps: S1, dry granulation of a medicine layer: preparing medicine layer particles from theophylline, a slow-release hydrophilic polymer material, a moisture absorbent, a flow aid and a lubricant; s2, performing dry granulation on the boosting layer: preparing boosting layer particles from a coloring material, an expansive high polymer material, a moisture absorbent, a flow aid and a lubricant; s3, tabletting of double-layer tablets: adding the medicine layer particles and the boosting layer particles into a double-layer tablet press for tabletting; s4, semi-permeable membrane coating: adding the theophylline double-layer tablet chip and a semi-permeable membrane coating solution into a coating machine; and S5, laser drilling: drilling holes in the theophylline double-layer semi-permeable membrane coating tablets through a laser drilling machine. The osmotic pump controlled release technology is utilized to control the constant-speed release of the medicine, the medicine effect is more lasting, the plasma concentration is stable, the adverse reaction of the medicine is effectively reduced, the medicine taking frequency is reduced, the medicine taking compliance of a patient is improved, and the clinical treatment effect of the medicine is improved.

The present invention provides methods for producing crystalline anti-PD-1 monoclonal antibodies (mAbs), wherein the mAbs are pembrolizumab or pembrolizumab variants, comprising (1) mixing a solution comprising (a) a mAb, (b) polyethylene glycol (PEG), and (c) an additive selected from the group consisting of caffeine, theophylline, 2'-deoxyguanosine-5'-monophosphate, a biologically active gibberellin, and a pharmaceutically acceptable salt of the biologically active gibberellin to form a crystallization solution; (2) incubating the crystallization solution for a time sufficient for crystal formation; and (3) optionally harvesting the crystalline anti-PD-1 mAb from the solution. In particular embodiments, the PEG is PEG 3350, and the additive is caffeine. The present invention also relates to novel anti-human PD-1 mAb crystals produced by the methods described herein. Characterization of the resolubilized crystal suspensions using several biochemical methods indicates that the biophysical properties of the resolubilized mAb crystals are consistent with the intact antibody starting sample. The crystals and methods of the present invention are suitable for a variety of pharmaceutical applications, such as purification, storage, formulation, and drug delivery.

The invention provides methods for producing crystalline an anti-PD-1 monoclonal antibody (mAb), wherein the mAb is pembrolizumab or a pembrolizumab variant, comprising (1) mixing a solution comprising (a) the mAb, (b) polyethylene glycol (PEG), and (c) an additive selected from the group consisting of: caffeine, theophylline, 2′ deoxyguanosine-5′-monophosphate, a bioactive gibberellin, and a pharmaceutically acceptable salt of said bioactive gibberellin, to form a crystallization solution, (2) incubating the crystallization solution for a period of time sufficient for crystal formation; and (3) optionally harvesting the crystalline anti-PD-1 mAb from the solution. In specific embodiments, the PEG is PEG 3350 and the additive is caffeine. The invention also relates to the novel anti-human PD-1 mAb crystals produced by the methods described herein. Characterization of re-dissolved crystalline suspensions using several biochemical methods showed the bio-physical properties of the re-dissolved mAb crystals were consistent with the intact antibody starting sample. The crystals and methods of the invention are amenable to multiple pharmaceutical applications such as purification, storage, formulation, and drug delivery.

The invention discloses a bacillus amyloliquefaciens riboswitch regulation and control system and application thereof. The regulation and control system is composed of a P43 promoter, a theophylline aptamer TC, RBS, RepA-tag and a required regulation and control gene. A theophylline ribose switch regulation element is integrated on the upstream of a bacillus amyloliquefaciens regulation gene, and the expression quantity of the gene is precisely regulated through theophylline induced expression. Furthermore, a theophylline ribose switch regulation element is integrated to the upstream of the genes hemX and mtnN to obtain the dual-regulation engineering bacterium HZ-B. Under induction of 2mM theophylline, the heme yield and the 5-adenosylmethionine (SAM) yield of the dual-regulation engineering bacterium HZ-B are remarkably increased and are respectively increased by 33.1 times and 0.5 times compared with those of an original strain HZ-12.

The application provides theophylline sustained-release tablets, a preparation method and application thereof, and belongs to the technical field of medicines.The theophylline sustained-release tablets comprise the following raw materials in parts by weight: theophylline derivatives or theophylline 90-110 parts;hydroxyethyl cellulose 10-20 parts;polyvinylpyrrolidone 1-2 parts;hexadecanol 3-5 parts;octadecanol 3-6 parts;talcum powder 0.2-0.6 parts;and magnesium stearate 0.2-0.6 parts.The preparation method is simple, the synthesis condition is mild, the yield is high, the theophylline derivatives have high selectivity, strong bronchodilating effect, quick effect, long maintenance time, small gastric irritation, slight cardiac side effect and good biological activities such as anti-tuberculosis, anti-convulsion and smooth muscle relaxation, and the theophylline derivatives are in a sustained-release dosage form, the drug action time is prolonged, and the theophylline derivatives have a wide application prospect.

The present invention provides a method for producing a crystalline anti-PD-1 monoclonal antibody (mAb) wherein the mAb is pembumab or a pembumab variant, the method comprising (1) mixing a solution comprising (a) mAb, (b) polyethylene glycol (PEG), and (c) an additive to form a crystalline solution, the additive is selected from caffeine, theophylline, 2 '-deoxyguanosine-5'-monophosphate, bioactive gibberellin and pharmaceutically acceptable salts of the bioactive gibberellin; (2) incubating the crystallization solution for a time sufficient for crystal formation; and (3) optionally harvesting the crystalline anti-PD-1mAb from the solution. In a particular embodiment, PEG is PEG 3350, and the additive is caffeine. The present invention also relates to novel anti-human PD-1mAb crystals produced by the methods described herein. Characterization of the redissolved crystal suspension using several biochemical methods shows that the biophysical properties of the redissolved mAb crystals coincide with the intact antibody starting sample. The crystals and methods of the present invention are suitable for a variety of pharmaceutical applications, such as purification, storage, formulation and drug delivery.

The disclosure relates to a method for eliminating interference of theophylline in immunoassay and an immunodetection kit. The method for eliminating interference of theophylline in immunoassay includes: performing an immunoassay in the presence of a theophylline interference-resistant agent, the theophylline interference-resistant agent having a final concentration of 0.1 mg / mL-100 mg / mL, and being selected as at least one component from a group consisting of free 6-mercaptopurine, 6-thioguanine, 8-azguanine, hypoxanthine, adenine, purine, 2,6,8-trioxypurine, adenine nucleotide, and adenosine. The method and the kit can effectively reduce interference of theophylline on immunoassay results.

Puerarin and theophylline co-crystal, preparation method thereof, and composition and use thereof. The present application discloses a co-crystal formed by combining puerarin (English name: Puerarin) and theophylline (English name: Theophylline) through non-covalent bonds, and a preparation method and use thereof. Specifically, the present application discloses a co-crystal formed by puerarin and theophylline, the molecular formula of the co-crystal is C 21 H 20 O9·C7H8N4O2; a preparation method of the puerarin and theophylline co-crystal; and application of the puerarin and theophylline co-crystal in preparation of a drug having effects of preventing and treating cardiovascular diseases, antioxidation, anticancer, antihypertension, blood sugar reduction, anti-inflammation, antipyretic analgesia, anti-Alzheimer's disease, anti-osteoporosis, cardiotonic, diuresis, coronary artery expansion, bronchial smooth muscle relaxation, and central nervous system excitation.

The invention provides a theophylline sustained-release tablet as well as a preparation method and application thereof, and belongs to the technical field of medicines. The theophylline sustained release tablet comprises the following raw materials in parts by weight: 90-110 parts of theophylline derivative or theophylline; 10 to 20 parts of hydroxyethyl cellulose; 1 to 2 parts of povidone; 3-5 parts of hexadecanol; 3 to 6 parts of octadecanol; 0.2 to 0.6 part of talcum powder; and 0.2 to 0.6 part of magnesium stearate. The preparation method is simple, the synthesis condition is mild, the yield is high, the prepared theophylline derivative has the advantages of high selectivity, strong bronchiectasis effect, quick response, long maintenance time, small stomach irritation, slight cardiac side effect and better biological activities of antituberculosis, anticonvulsion, smooth muscle relaxation and the like, meanwhile, the theophylline derivative is in a sustained-release dosage form, the medicine action time is prolonged, and the bioavailability of the theophylline derivative is improved. Wide application prospects are realized.

The invention discloses a diabetes typing marker based on a D2D3-TROY signal axis, a new medical application of valprophylline and a kit for diagnosing a specific diabetes subtype.

The invention relates to the technical field of carbon nanomaterials, and discloses a preparation method of nitrogen-doped carbon quantum dots with high fluorescence quantum yield, which comprises the following steps: dissolving theophylline drugs and moderate-intensity organic acid in distilled water, stirring until the theophylline drugs and the moderate-intensity organic acid are completely dissolved, and pouring the obtained mixed solution into a reaction kettle; carrying out heat treatment on the mixed solution in the reaction kettle, and then naturally cooling to room temperature along with the furnace to obtain a target solution; the target solution is subjected to centrifugal treatment to obtain layered liquid, supernate on the upper layer of the layered liquid is lifted, and the nitrogen-doped carbon quantum dots with the high fluorescence quantum yield are obtained. The nitrogen-doped carbon quantum dots with high fluorescence quantum yield are simply and easily synthesized by using a hydrothermal synthesis method, and the carbon nanomaterial with outstanding fluorescence performance is expected to be important in application in multiple aspects such as environmental monitoring ion detection, medical image cell marking and tracking and the like.

The application discloses a method for screening high-quality high-theophylline camellia sinensis single plant based on a resistance character-chemical fingerprint-SSR molecular marker, which comprises the following steps: (1) field resistance character comprehensive evaluation is carried out on camellia sinensis natural hybridization offspring seed seedlings, and single plants with strong disease resistance, insect resistance, cold resistance and drought resistance are screened; (2) the theophylline content of the screened single plants is determined, and the theophylline content greater than or equal to 1.0% is taken as a screening standard; (3) SSR fluorescent marker primers are used for PCR amplification, capillary electrophoresis detection is carried out on the PCR products, the genetic similarity coefficients and the genetic relationship among the single plants are analyzed, and the single plants with rich genetic diversity are reserved. The application firstly constructs a three-level screening system of "resistance character primary screening-chemical secondary screening-SSR genetic diversity analysis", evaluates the genetic background difference of candidate single plants through the SSR marker, and screens the single plants with rich genetic diversity, so as to provide high-quality germplasm resources with rich genetic foundation for subsequent camellia sinensis variety breeding.

Adenosine A2A receptor antagonist are useful in methods for treating or preventing REM sleep behavior disorder, treating or preventing REM sleep without atonia, and preventing or delaying onset of a synucleinopathy (e.g., Parkinson's disease, dementia with Lewy bodies, multiple system atrophy, pure autonomic failure, Alzheimer's disease with amygdalar restricted Lewy bodies). In embodiments, the the adenosine A2A receptor antagonist is ciforadenant, enprofylline. vipadenant, preladenant. tozadenant. istradefylline, inupadenant, etrumadenant, taminadenant, TB206-001, PORT-6, M1069, ST1535, or ST4206.

The invention discloses a nitrate drug metabolic enzyme activity quantitative detection method in the technical field of drug metabolism and individualized medication, which comprises the following steps: incubating a reaction system containing theophylline acetaldehyde or a hydrate thereof with whole blood of an individual to be detected, and detecting the amount of theophylline acetic acid generated by the theophylline acetaldehyde through specific metabolism of ALDH1A1 and ALDH2 in the system by adopting LC-MS / MS (Liquid Chromatography-Mass Spectrometry / Mass Spectrometry); the metabolic clearance ability is assessed based on the amount of production. Whole blood is used as an enzyme source, blood cells do not need to be separated, the process is simple and convenient, the actual activity of in-vivo related enzymes can be directly and quantitatively reflected, the limitation of traditional genotype detection is overcome, clinical medication of nitrate drugs can be accurately guided, and drug failure and tolerance risks can be predicted.

The invention discloses a preparation method of a theophylline-platinum (IV) complex and application of the theophylline-platinum (IV) complex in tumor drugs, one molecule of theophylline is introduced to a single side in the axial direction of a platinum (IV) coordination center or two molecules of theophylline are introduced to two sides of the platinum (IV) coordination center to form a monosubstituted tetravalent platinum complex or a symmetrical disubstituted tetravalent platinum complex; or, one molecule of theophylline is introduced to one axial side of the platinum (IV) coordination center, and one molecule of carbon long-chain group is introduced to the other axial side of the platinum (IV) coordination center, so that the asymmetric disubstituted tetravalent platinum complex is formed, the prepared compound has stronger killing ability on tumor cells, and the anti-proliferative activity in various cell lines is obviously improved. The theophylline-platinum (IV) complex disclosed by the invention is simple in synthesis process and low in cost, greatly improves the curative effect of drug combination, and has the advantages of good curative effect, small side effect and the like compared with traditional bivalent platinum and similar tetravalent platinum drugs.

The invention relates to the field of caffeine synthesis, and discloses a caffeine synthesis method capable of removing green fluorescent impurities, which comprises the following steps: (1) adding acid into dimethyl DAU, and adjusting the pH value to 2-6; (2) extracting the dimethyl DAU solution by using an extracting agent under an acidic condition, and removing green fluorescent impurities in the dimethyl DAU solution; (3) adjusting the pH value of the extracted dimethyl DAU solution to 6-7 by using a NaOH solution, and then carrying out formylation reaction on the dimethyl DAU solution and formic acid to generate dimethyl FAU; and (4) carrying out ring-closure reaction on the dimethyl FAU under an alkaline condition to generate theophylline sodium, directly carrying out methylation reaction on the theophylline sodium and dimethyl sulfate to generate caffeine, crystallizing and centrifuging to obtain a caffeine crude product, and refining to obtain a caffeine product. According to the synthetic method disclosed by the invention, green fluorescent impurities which seriously influence the appearance quality of a caffeine finished product can be effectively removed, the operation of crystallizing and separating theophylline sodium salt is omitted, and wastewater discharge is reduced.