17 results about "Theophylline" patented technology

The invention belongs to the technical field of medicine detection, and particularly relates to an ICPMS (Inductively Coupled Plasma Mass Spectrometry) detection method for element impurities in theophylline. The linear test solution, the blank solution and the theophylline test solution are subjected to ICPMS detection, the content of element impurities in the theophylline test solution is calculated according to an internal standard curve method, and the element impurities are one or more of cadmium, lead, arsenic, mercury, cobalt, vanadium, nickel, lithium, antimony or copper. The method is good in reproducibility and stability, high in accuracy and high in precision.

The present application provides a composition with lipid-lowering and weight-reducing functions, which has clear components and controllable quality. The composition comprises Pu'er tea extract, wherein the Pu'er tea extract contains 10-90% theabrownine by weight, preferably also contains theophylline, the weight ratio of the theabrownine and theophylline is 10:1-60:1, and contains 0-15% tea polyphenols by weight. The theophylline can be selected from exogenous theophylline or theophylline contained in the Pu'er tea extract. The composition with lipid-lowering and weight-reducing functions of the present application also comprises Poria cocos extract; the weight ratio of the Pu'er tea extract to the Poria cocos extract is 1:3-3:1 based on the weight of theabrownine contained in the Pu'er tea extract. The composition of the present application has the advantages of clear effective components, simple preparation process, controllable quality, and stable lipid-lowering and weight-reducing effects.

This invention belongs to the field of photoelectrochemical detection, specifically relating to a method for fabricating a photo-assisted all-solid-state zinc-air battery self-powered sensor based on a signal reversal strategy and its application in detecting theophylline. Using CuInS2 as the photocathode, CdS is loaded onto the photocathode using a split aptamer technique. Polished zinc foil is used as the anode, and PVA gel polymer is used as the solid electrolyte to construct a photo-assisted all-solid-state zinc-air battery self-powered sensor based on a signal reversal strategy. This invention achieves signal reversal by incorporating split aptamer technology, thereby significantly improving the detection sensitivity for antioxidants. The constructed photo-assisted all-solid-state zinc-air battery self-powered sensor based on a signal reversal strategy also has a wide detection range, low detection limit, and low detection cost.

The invention belongs to the technical field of molecular biology, and particularly discloses a theophylline aptamer and a virtual screening method and application thereof. The theophylline aptamer is selected from one or more of MUT9, MUT16, MUT67, MUT78 or MUT89. The invention further discloses a preparation method of the theophylline aptamer. The invention discloses a theophylline aptamer as well as a virtual screening method and application thereof, the theophylline aptamer is high in affinity and specificity, the virtual screening method remarkably improves the accuracy and efficiency of virtual screening, the theophylline aptamer can be quickly obtained at low cost, and a powerful calculation tool is provided for rational design and application of the aptamer.

The invention relates to a preparation method of a caffeine impurity C. The preparation method of the caffeine impurity C can effectively solve the problems of preparation of the caffeine impurity C, effective control of impurity content in caffeine and guarantee of coffee quality, and the preparation method comprises the following steps: mixing theophylline, a catalyst triethylene diamine, N, N-dimethylformamide and dimethyl carbonate, heating for reaction, distilling, adding water for recrystallization, and filtering to obtain the caffeine impurity C. Recrystallized jellyfish liquid is obtained; and adding a halogenated hydrocarbon solvent, standing until a solid is separated out between the water layer and the halogenated hydrocarbon solvent layer, filtering, and drying to obtain the product. The invention provides the caffeine impurity preparation method which is simple and easy to implement, mild in condition and low in energy consumption and pollution, so that the preparation vacancy of the impurity at present is filled up, powerful technical support is provided for quality research and quality control of caffeine, and the method has practical popularization and application values.

The invention discloses a method for simultaneously sensing and detecting two components of theophylline / caffeine based on a derived carbon-based material, the detection linear ranges of theophylline and caffeine are respectively 20-500 [mu] mol / L and 10-500 [mu] mol / L, the detection limits are respectively as low as 0.017 [mu] M and 0.021 [mu] M, and the sensitivities are respectively as high as 19.97 [mu] A / mM and 11.39 [mu] A / mM. Moreover, the sensor has the characteristics of high selectivity, good reproducibility, good stability and the like when being used for simultaneously detecting the theophylline and the caffeine, and is successfully used for simultaneously detecting the theophylline and the caffeine in the health-care food weight-reducing tea. It is worthy of mentioning that the nano material is easy and convenient to prepare and operate and short in consumed time, meanwhile, the nano material has the advantages of being low in raw material cost, wide and easy to obtain and the like, waste is turned into wealth, and the concept of sustainable development and green environmental protection is met.

The invention discloses a theophylline alkaloid salt, a pharmaceutical composition thereof, a preparation method of the theophylline alkaloid salt, and an application of the theophylline alkaloid salt, and belongs to the technical field of pharmaceutical chemistry. Natural alkaloids with definite pharmacological activity, such as stachydrine, betaine, trigonelline, sophocarpidine, choline or berberine, are selected as saline-forming bases, and a series of novel theophylline alkaloid salts are developed. The functional salt formation strategy not only fundamentally changes the traditional theophylline salt formation mode, but also realizes a leap-through breakthrough from single improvement of solubility to synergistic interaction and active attenuation. Through inherent gastric mucosa protection, heart function regulation and other effects of alkaloid, an active attenuation mechanism is constructed, the core toxic and side effects of gastrointestinal irritation, heart excitability and the like caused by theophylline are effectively reduced, the risk of introduction of exogenous sensitizers is avoided, and the bioavailability of theophylline is improved. The clinical problems of narrow therapeutic window and large toxic and side effects of traditional theophylline and salt drugs thereof are effectively solved.

A pharmaceutical composition in the form of a transdermal patch comprising aminophylline or theophylline for use in the treatment or prevention of a hypomyelinating or a demyelinating disease or condition or a lesion of the peripheral or central nervous system where demyelination occurs. The patch may comprise a matrix layer comprising a matrix polymer, an active ingredient and a permeation enhancer.

The invention provides methods for producing crystalline an anti-PD-1 monoclonal antibody (mAb), wherein the mAb is pembrolizumab or a pembrolizumab variant, comprising (1) mixing a solution comprising (a) the mAb, (b) polyethylene glycol (PEG), and (c) an additive selected from the group consisting of: caffeine, theophylline, 2′ deoxyguanosine-5′-monophosphate, a bioactive gibberellin, and a pharmaceutically acceptable salt of said bioactive gibberellin, to form a crystallization solution, (2) incubating the crystallization solution for a period of time sufficient for crystal formation; and (3) optionally harvesting the crystalline anti-PD-1 mAb from the solution. In specific embodiments, the PEG is PEG 3350 and the additive is caffeine. The invention also relates to the novel anti-human PD-1 mAb crystals produced by the methods described herein. Characterization of re-dissolved crystalline suspensions using several biochemical methods showed the bio-physical properties of the re-dissolved mAb crystals were consistent with the intact antibody starting sample. The crystals and methods of the invention are amenable to multiple pharmaceutical applications such as purification, storage, formulation, and drug delivery.

The invention discloses a bacillus amyloliquefaciens riboswitch regulation and control system and application thereof. The regulation and control system is composed of a P43 promoter, a theophylline aptamer TC, RBS, RepA-tag and a required regulation and control gene. A theophylline ribose switch regulation element is integrated on the upstream of a bacillus amyloliquefaciens regulation gene, and the expression quantity of the gene is precisely regulated through theophylline induced expression. Furthermore, a theophylline ribose switch regulation element is integrated to the upstream of the genes hemX and mtnN to obtain the dual-regulation engineering bacterium HZ-B. Under induction of 2mM theophylline, the heme yield and the 5-adenosylmethionine (SAM) yield of the dual-regulation engineering bacterium HZ-B are remarkably increased and are respectively increased by 33.1 times and 0.5 times compared with those of an original strain HZ-12.

The application provides theophylline sustained-release tablets, a preparation method and application thereof, and belongs to the technical field of medicines.The theophylline sustained-release tablets comprise the following raw materials in parts by weight: theophylline derivatives or theophylline 90-110 parts;hydroxyethyl cellulose 10-20 parts;polyvinylpyrrolidone 1-2 parts;hexadecanol 3-5 parts;octadecanol 3-6 parts;talcum powder 0.2-0.6 parts;and magnesium stearate 0.2-0.6 parts.The preparation method is simple, the synthesis condition is mild, the yield is high, the theophylline derivatives have high selectivity, strong bronchodilating effect, quick effect, long maintenance time, small gastric irritation, slight cardiac side effect and good biological activities such as anti-tuberculosis, anti-convulsion and smooth muscle relaxation, and the theophylline derivatives are in a sustained-release dosage form, the drug action time is prolonged, and the theophylline derivatives have a wide application prospect.

The disclosure relates to a method for eliminating interference of theophylline in immunoassay and an immunodetection kit. The method for eliminating interference of theophylline in immunoassay includes: performing an immunoassay in the presence of a theophylline interference-resistant agent, the theophylline interference-resistant agent having a final concentration of 0.1 mg / mL-100 mg / mL, and being selected as at least one component from a group consisting of free 6-mercaptopurine, 6-thioguanine, 8-azguanine, hypoxanthine, adenine, purine, 2,6,8-trioxypurine, adenine nucleotide, and adenosine. The method and the kit can effectively reduce interference of theophylline on immunoassay results.

Puerarin and theophylline co-crystal, preparation method thereof, and composition and use thereof. The present application discloses a co-crystal formed by combining puerarin (English name: Puerarin) and theophylline (English name: Theophylline) through non-covalent bonds, and a preparation method and use thereof. Specifically, the present application discloses a co-crystal formed by puerarin and theophylline, the molecular formula of the co-crystal is C 21 H 20 O9·C7H8N4O2; a preparation method of the puerarin and theophylline co-crystal; and application of the puerarin and theophylline co-crystal in preparation of a drug having effects of preventing and treating cardiovascular diseases, antioxidation, anticancer, antihypertension, blood sugar reduction, anti-inflammation, antipyretic analgesia, anti-Alzheimer's disease, anti-osteoporosis, cardiotonic, diuresis, coronary artery expansion, bronchial smooth muscle relaxation, and central nervous system excitation.

The invention discloses a diabetes typing marker based on a D2D3-TROY signal axis, a new medical application of valprophylline and a kit for diagnosing a specific diabetes subtype.

The application discloses a method for screening high-quality high-theophylline camellia sinensis single plant based on a resistance character-chemical fingerprint-SSR molecular marker, which comprises the following steps: (1) field resistance character comprehensive evaluation is carried out on camellia sinensis natural hybridization offspring seed seedlings, and single plants with strong disease resistance, insect resistance, cold resistance and drought resistance are screened; (2) the theophylline content of the screened single plants is determined, and the theophylline content greater than or equal to 1.0% is taken as a screening standard; (3) SSR fluorescent marker primers are used for PCR amplification, capillary electrophoresis detection is carried out on the PCR products, the genetic similarity coefficients and the genetic relationship among the single plants are analyzed, and the single plants with rich genetic diversity are reserved. The application firstly constructs a three-level screening system of "resistance character primary screening-chemical secondary screening-SSR genetic diversity analysis", evaluates the genetic background difference of candidate single plants through the SSR marker, and screens the single plants with rich genetic diversity, so as to provide high-quality germplasm resources with rich genetic foundation for subsequent camellia sinensis variety breeding.

The invention discloses a nitrate drug metabolic enzyme activity quantitative detection method in the technical field of drug metabolism and individualized medication, which comprises the following steps: incubating a reaction system containing theophylline acetaldehyde or a hydrate thereof with whole blood of an individual to be detected, and detecting the amount of theophylline acetic acid generated by the theophylline acetaldehyde through specific metabolism of ALDH1A1 and ALDH2 in the system by adopting LC-MS / MS (Liquid Chromatography-Mass Spectrometry / Mass Spectrometry); the metabolic clearance ability is assessed based on the amount of production. Whole blood is used as an enzyme source, blood cells do not need to be separated, the process is simple and convenient, the actual activity of in-vivo related enzymes can be directly and quantitatively reflected, the limitation of traditional genotype detection is overcome, clinical medication of nitrate drugs can be accurately guided, and drug failure and tolerance risks can be predicted.