24 results about "Nuciferine" patented technology

The invention discloses lotus leaf powder as well as a preparation method and application thereof, and belongs to the technical field of deep processing of plant raw materials. The preparation method of the lotus leaf powder provided by the invention comprises the following steps: carrying out enzymolysis on pretreated fresh lotus leaves in a system containing beta-glucosidase, and then carrying out scalding and ice water leaching cooling; the obtained lotus leaf powder has bright emerald green color, and contains more active ingredients such as flavonoid aglycones and nuciferine which are beneficial to human body absorption; moreover, the content of tannin and total flavonoid glycoside in the obtained lotus leaf powder is relatively low, so that the lotus leaf powder has relatively low bitter and astringent taste, and the obtained lotus leaf powder is excellent in mouth feel; meanwhile, the obtained lotus leaf powder also has good capability of improving blood fat; the method can be widely applied to preparation of matcha-flavored food or preparation of functional preparations for improving blood fat.

The application discloses a supramolecular lotus leaf peptide-narcotic alkaloid Pickering emulsion as well as a preparation method and application thereof. The water phase of the supramolecular lotus leaf peptide-narcotic alkaloid Pickering emulsion is a purified lotus leaf peptide aqueous solution, and the oil phase is camellia seed oil loaded with narcotic alkaloids. The camellia seed oil not only serves as a fat-soluble carrier to improve the solubility of the narcotic alkaloids, but also has nursing effects due to the unsaturated fatty acids, vitamin E and phytosterols contained therein. The supramolecular assembly technology is used to mediate the lotus leaf peptide and the narcotic alkaloids to form high-performance emulsified particles, to replace synthetic surfactants to stabilize the Pickering emulsion, and to construct a natural emulsion system with high emulsification efficiency, high stability and high performance, so that the performance and the nursing effect of the emulsion are optimized.

The application discloses a nuciferine derivative, a preparation method and application thereof, and relates to the technical field of medicines.The application provides a nuciferine derivative, and further provides a preparation method of the nuciferine derivative.The nuciferine derivative provided by the application greatly improves the treatment effect of arrhythmia, reduces the adverse reaction of the drug, and reduces the risk of inducing ventricular arrhythmia; in addition, the raw material is cheap and easy to obtain, the production cost is greatly reduced, the nuciferine derivative is suitable for large-scale production, and the nuciferine derivative has a certain treatment effect on QT syndrome, and has a wide application prospect in the preparation of drugs for resisting arrhythmia and treating QT syndrome.

The invention discloses an N-aryl nuciferine derivative and a preparation method and application thereof.The chemical structure of the N-aryl nuciferine derivative is shown in the formula I. In the formula I, an aromatic ring A is selected from a benzene ring, a naphthalene ring, biphenyl or nitrogen heteroaromatic ring; r is selected from an electron-donating group and an electron-withdrawing group, or two ends of R are connected with an aromatic ring A to form a ring, the substitution number of the electron-donating group is 1-2, and the substitution number of the electron-withdrawing group is 1. The N-arylated nuciferine derivative disclosed by the invention has anti-lipase activity superior to that of a parent compound, can inhibit lipid accumulation in a cell differentiation process, and has further development potential.

The invention discloses a method for simultaneously determining the contents of five components in a medicinal and edible beverage and application thereof. The method for simultaneously determining the contents of rutin, nuciferine, sinapine, liquiritin and glycyrrhizic acid in the medicinal and edible beverage prepared from mulberry leaves, lotus leaves, mustard seeds and licorice roots under the same chromatographic condition by adopting a high performance liquid chromatography comprises the following steps: firstly preparing a water extract, a mixed reference solution and a test solution, and then detecting under a specific chromatographic condition to realize determination, so that the content of rutin, nuciferine, sinapine, liquiritin and glycyrrhizic acid in the medicinal and edible beverage is determined. The method is simple and easy to operate, strong in specificity, repeatable, good in precision and stability, and capable of comprehensively reflecting the quality of the product. The method can also be used for standardized extraction and preparation of the medicinal and edible beverage. Through simultaneous determination of multiple indexes, an important method guarantee is provided for effective extraction and quality control of the medicinal and edible beverage with lower cost, higher popularity and more flexible operation space, the blank of quality control of multiple components in the medicinal and edible beverage is made up, and a preferable extraction process can be applied to production and has a wide application prospect. The practical application and popularization value is realized.

The invention discloses a walnut peptide compound plant solid beverage capable of reducing uric acid as well as a preparation method and application of the walnut peptide compound plant solid beverage. The walnut enzymolysis mixed peptide is taken as a core, so that the absorption efficiency of active ingredients is remarkably improved, and the overall functional effect can be enhanced. By combining with multiple plant functional components such as sour cherry, chicory, dandelion, celery and nuciferine, a synergistic effect can be formed in multiple links such as inhibiting generation of uric acid, promoting excretion and improving a metabolic environment, so that the uric acid reducing effect better than that of a single plant component is realized. Freeze-drying, low-temperature processing and proper plant extraction processes are adopted, so that plant active substances are reserved to the greatest extent, and the product stability is improved; the preparation process is simple, suitable for large-scale production, stable in product form, good in solubility and convenient to carry and eat for a long time.

The present application relates to the technical field of pharmaceutical preparation, in particular to a lotus leaf alkaloid liposome emulsion, a preparation method and application thereof, wherein sucrose / sodium chloride solid particles are combined with a wrapping material by a dipping film formation method to prepare empty liposomes, and a drug lotus leaf alkaloid is coated to prepare a lotus leaf alkaloid liposome emulsion.The preparation method provided by the present application is simple in process; raw materials are widely sourced and green and environmentally friendly, without affecting the biological activity of active ingredients; the preparation process is mild in conditions, without the need for special equipment, without changing the pH value of the solution, suitable for transdermal and oral administration; the amount of organic solvent is small, the encapsulation rate is high, and the preparation is easy to scale up.

The invention belongs to the technical field of microbial fermentation, and relates to lipid-lowering type fermented lotus leaf powder and a preparation method and application thereof.The preparation method comprises the following steps that water is added into lotus leaves according to the material-liquid ratio of 1: 10-30, then a proper amount of carbon source is added, the mixture is heated for 15-60 min at the temperature of 75-100 DEG C, and the temperature is reduced to 35-40 DEG C for use; inoculating lactobacillus plantarum LP-KFY04 and lactobacillus rhamnosus bv-77, and fermenting for 8 to 72 hours at the temperature of 28 to 40 DEG C; when the pH value of the solution is reduced to 4.5 and the reducing sugar is less than 0.5%, ending the fermentation; raising the temperature to 90-95 DEG C, and extracting to obtain a first extracting solution; adding water according to the material-liquid ratio of the lotus leaves to the water being 1: (6-30), carrying out second heating extraction to obtain a second extracting solution, and combining the extracting solutions; according to the method, active substances such as nuciferine, flavone and polysaccharide can be synchronously and efficiently extracted, the taste and flavor of the product are improved, and the effects of lowering lipid and losing weight are enhanced.

The invention discloses liver-soothing and depression-relieving type medicinal and edible weight-reducing tea based on AMPK / SREBP-1c pathway regulation and control and a preparation method of the weight-reducing tea. A targeted extraction technology is introduced and comprises the steps of step-by-step extraction, embedding purification, liquid phase preparation and the like, key components, such as hawthorn flavone, nuciferine and the like, acting on an SREBP-1c pathway are accurately extracted, the key components and medicinal and edible components such as ginseng, poria cocos and the like in a formula have a synergistic effect, AMPK phosphorylation is effectively activated, SREBP-1c nuclear translocation is inhibited, and the SREBP-1c protein is prepared. Deeper lipid metabolism regulation and control and liver-intestinal axis balance are realized. Cell and animal experiments show that the tea can significantly reduce accumulation of triglyceride and has no side effects of drugs.

The invention provides a lotus leaf extract, a preparation method thereof and application of the lotus leaf extract as an active ingredient of a sphingosine kinase 1 agonist, the lotus leaf extract is obtained by mixing, fermenting and culturing lotus leaf powder and bacterial strain fermentation liquor of bacillus subtilis, and the lotus leaf extract is enriched with nuciferine with higher content. The nano vesicles which are uniformly distributed and have the average diameter of 90 to 180nm are reserved; the lotus leaf extract can be used as an SPHK1 agonist, and an autophagy-lysosome pathway is activated by activating an SPHK1 pathway, so that abnormal accumulation of cell lipid is effectively inhibited, and a new thought is provided for research and development of skin oil control products.

The present invention belongs to the field of drug synthesis, and in particular relates to a URAT1-inhibiting aromatic acyl nuciferine derivative and its preparation method and application. The present invention structurally modifies nuciferine by introducing an active group, thereby improving the biological activity of nuciferine in lowering uric acid. Cell experiments have found that the aromatic acyl nuciferine derivative prepared by the present invention can inhibit the expression of URAT1 in HK-2 cells, promote uric acid excretion, thereby reducing uric acid levels, which is of great significance for the further development of new URAT1 inhibitors. The aromatic acyl nuciferine derivative with uric acid-lowering and kidney-protecting effects provided by the present invention has a simple synthesis method, low raw material price, and novel structure.

The invention discloses a use method of a nuciferine-containing rheumatoid arthritis bone erosion resistant composition, and relates to the field of rheumatoid arthritis bone erosion treatment.The use method comprises the steps that 1, an applicable object is determined, and the applicable object is a patient suffering from rheumatoid arthritis and having the bone erosion condition; according to the invention, the applicable object is determined to be a patient with rheumatoid arthritis and bone erosion, so that the treatment is more targeted, unnecessary use of medicines in non-target people is avoided, the utilization efficiency of medical resources is improved, and potential risks possibly brought by the medicines to non-applicable people are reduced; the nuciferine not only has remarkable anti-inflammatory activity in vitro, but also remarkably relieves the process of CIA rat arthritis in vivo, and the nuciferine relieves inflammatory response by reducing expression levels of inflammatory factors TNF-alpha, IL-1beta and IL-6 and total IgG, IgG1 and IgG2a of immune globulins in CIA rat serum.

The invention relates to the technical field of nutrition injections, in particular to a nutrition injection for fruit tree trunks and a preparation method of the nutrition injection. The nutrition injection is prepared from the following raw materials: urea, a compound nutrient, an additive solution, cane sugar and water. In the preparation process of the nutrition injection for the fruit tree trunks, the ginkgo leaves, the rosemary and the lotus leaves are combined and added, and bilobalide components of the ginkgo leaves can promote division and elongation of root cells of fruit trees, so that the root systems of the fruit trees are more developed, and the water and nutrient absorption capacity of the fruit trees after transplanting is enhanced; rosmarinic acid has the effect of adjusting plant hormone balance and can stimulate fruit trees to root again at damaged positions of root systems after transplanting and improve the activity and absorption efficiency of the root systems, nuciferine is beneficial to improving the growth environment of the fruit tree root systems and promoting microbial activity of the fruit tree root systems, good conditions are provided for growth of the fruit tree root systems, and the yield of the fruit trees is increased. The yield and the transplanting survival rate of the fruit trees are effectively improved.

The invention belongs to the technical field of medicines, and finds that aporphine alkaloids, especially magnoline and nuciferine, can reduce the BUN level and SCr level of a cisplatin-induced acute kidney injury model mouse through a large number of experimental screening, can obviously relieve cisplatin-induced kidney function impairment, and can be used for preparing medicines for treating acute kidney injury. The compound can be used as a medicine for reducing adverse reaction of renal function impairment caused by cis-platinum.

The application relates to the field of material analysis, and discloses a test method for analyzing the properties of lotus plumule extract, which comprises the following steps: introducing the lotus plumule extract to be detected into a flow detection pool to establish a transient electrically induced birefringence monitoring field; applying a high-frequency bipolar symmetric square wave electric field to an excitation electrode pair; using the polarity high-frequency switching characteristic to limit the displacement of free ions within a local perturbation range, and simultaneously inducing the electric dipole orientation arrangement of alkaloid molecules; removing the electric field; monitoring and recording the transient birefringence decay trajectory data; and processing the data to separate the spin relaxation time constants belonging to different components, and determining the contents of nuciferine, isonor-lausplumuline and methylnuciferine according to the mapping rule between the constants and the effective hydrodynamic radius of molecules. The application realizes the physical decoupling of overlapping signals in the time domain through the spin relaxation characteristic, effectively suppresses the ionization interference of a complex matrix, and ensures the stability of a detection baseline.

The invention belongs to the technical field of biology, and particularly relates to application of nuciferine in preparation of a medicine for relieving pyroptosis of trophoblasts in preeclampsia. Nuciferine inhibits activation of NLRP3 inflammasomes and reduces caspase-1 cleavage and GSDMD mediated pyroptosis, so that placenta inflammation and dysfunction are relieved, and preeclampsia symptoms are improved. The nuciferine remarkably improves the symptoms of fetal growth limitation and placenta weight reduction of preeclampsia mice, and reduces placenta oxidative stress and inflammation level. In a cell model, nuciferine effectively reverses the pyroptosis phenotype of trophoblast cells, and the protein expression of NLRP3, cleaved-caspase-1, GSDMD-NT and IL-1beta is remarkably reduced. The application potential of nuciferine as a promising natural medicine in preeclampsia treatment is clarified, and the understanding of the relationship between inflammation and pyroptosis in preeclampsia pathogenesis is deepened.

The invention discloses a delta6a, 7 nuciferine N-o-methylbenzene derivative and a preparation method and application thereof.The chemical structure of the delta6a, 7 nuciferine N-o-methylbenzene derivative is shown in the formula I. In the formula I, an aromatic ring A is selected from a benzene ring, a naphthalene ring, biphenyl or nitrogen heteroaromatic ring; r is selected from an electron donating group or an electron withdrawing group. According to the invention, by adopting a metal palladium catalytic system and exploring different catalytic conditions, optimized conditions for synthesizing the delta6a, 7 nuciferine N-o-methylbenzene derivative are obtained. The delta6a, 7 nuciferine N-o-methylbenzene derivative disclosed by the invention has fat cell lipid accumulation inhibition activity, and has development potential in anti-obesity drugs.

The application discloses a kind of experimental mouse feeding control system applied to nuciferine research, it is related to intelligent experimental control technical field, it includes control module and the indicator light, detection mechanism and display electrically connected to control module, detection mechanism is installed in breeding cage and is used to detect feeding behavior, it includes mesh plate and weighing sensor, load-bearing platform is arranged below mesh plate, weighing sensor is installed on load-bearing platform, mesh plate is located on weighing sensor, feed trough in breeding cage is located on mesh plate, mesh plate is placed back to position after experimental mouse is taken out;Indicator light is two and is respectively installed in breeding cage, indicator light is RGB lamp type, control module is based on the weight data fed back by weighing sensor and whether the color of indicator light is switched within the preset time length to control indicator light to switch color and prompt.The present application has the effect of improving the success rate of nuciferine animal experiment.

The invention provides a lipid-lowering composite solid beverage and a preparation method thereof, and belongs to the technical field of lipid-lowering beverages. The composite solid beverage for lowering lipid comprises the following components in parts by weight: 10-20 parts of a mulberry leaf extract, 10-15 parts of a lotus leaf extract and 3-5 parts of a green tea extract, wherein the content of 1-DNJ in the mulberry leaf extract is greater than or equal to 0.5%, the content of flavonoid compounds is greater than or equal to 10%, the content of nuciferine in the lotus leaf extract is greater than or equal to 3%, and the content of EGCG in the green tea extract is greater than or equal to 50%. The composite solid beverage for reducing blood fat and the related composition thereof have obvious advantages in the aspects of blood fat reducing effect, component stability, taste and the like, and have wide market prospects and application values.

The invention relates to the technical field of medicine, and discloses a nuciferine-based nano preparation for targeting digestive tract tumors and a preparation method thereof.The nuciferine-based nano preparation for targeting digestive tract tumors comprises nuciferine, 1, 2-dipalmitoyl-sn-glycerol-3-phosphatidylcholine, cholesterol, cholesterol hemisuccinate, 1, 2-dipalmitoyl-sn-glycerol-3-phosphatidylcholine, cholesterol hemisuccinate, 1, 2-dipalmitoyl-sn-glycerol-3-phosphatidylcholine, cholesterol hemisuccinate, 1 the lipid vesicle is composed of 1, 2-dioleoyl-sn-glycerol-3-phosphatidyl ethanolamine and distearoyl phosphatidyl ethanolamine-polyethylene glycol (2000)-cyclo (essence-glycerol-radix asparagi-styrene-acrylic-lysine) polypeptide, and the lipid vesicle is composed of 2, 2-dioleoyl-sn-glycerol-3-phosphatidyl ethanolamine and distearoyl phosphatidyl ethanolamine-polyethylene glycol (2000)- The preparation method of the nuciferine-based digestive tract tumor targeting nano preparation comprises the steps of film dispersion, hydration, high-pressure extrusion and purification. The active targeting effect of the cyclo (essence-glycerol-asparagi-styrene-acrylic-lysine) polypeptide ligand is utilized. Synergistic release of drugs in a tumor microenvironment is achieved, the targeting efficiency and local drug concentration of nuciferine are improved, and the anti-tumor effect is enhanced.

The invention discloses a laboratory mouse feeding control system applied to nuciferine research, and relates to the technical field of intelligent experiment control, the laboratory mouse feeding control system comprises a control module, and an indicating lamp, a detection mechanism and a display which are electrically connected to the control module, the detection mechanism is installed in a feeding cage and used for detecting feeding behaviors, and the detection mechanism comprises a screen plate and a weighing sensor; a bearing table is arranged below the net plate, a weighing sensor is installed on the bearing table, the net plate is located on the weighing sensor, a feed trough in the rearing cage is located on the net plate, and the net plate is a position where laboratory mice are put back after being taken out; the number of the indicator lamps is two, the indicator lamps are installed on the feeding cages respectively, the indicator lamps are RGB lamp types, and the control module controls the indicator lamps to switch the colors for prompting based on weight data fed back by the weighing sensor and whether the colors of the indicator lamps are switched or not within the preset duration. The method has the effect of improving the success rate of the nuciferine animal experiment.

The invention discloses an experimental mouse metabolism monitoring system applied to nuciferine research, and relates to the technical field of intelligent management of nuciferine research, and the experimental mouse metabolism monitoring system comprises a positioning mechanism which is arranged in an experimental mouse container and is used for temporarily positioning an experimental mouse; an automatic injection machine; the gas environment creating mechanism is used for controlling gas components in the laboratory mouse container; a vision mechanism; a controller; the automatic injection mechanism comprises a driving unit and an injector, the outer wall of the injector is provided with an ID, the driving unit is installed on a top cover of the laboratory mouse container and used for driving the injector to act, the injector is detachably connected to the top cover of the laboratory mouse container and the driving unit, and the needle end of the injector extends into the laboratory mouse container; and the controller is configured to control the driving unit to drive the injector to collect blood from the laboratory mouse, establish a one-to-one correspondence relationship between the laboratory mouse and the injector identification ID based on feedback data of the visual mechanism, and generate a sample report. The method has the effect of improving the convenience of monitoring glycolipid metabolism of the experimental mouse.

The invention belongs to the technical field of medical gel, and particularly relates to antibacterial gel for obstetrics and a preparation method of the antibacterial gel. The antibacterial gel is prepared from the following raw materials in parts by weight: 0.3 to 0.5 part of nuciferine derivatives, 0.5 to 1.5 parts of hydroxyethyl cellulose, 0.5 to 1 part of glycerol, 1.5 to 2 parts of propylene glycol, 0.5 to 1.5 parts of carbomer, 70 to 80 parts of ethanol and 15 to 20 parts of water. The nuciferine derivative has the following structural formula. The antibacterial gel provided by the invention has good antibacterial performance and moisturizing effect.

Provided are a product for treating Helicobacter pylori-negative chronic atrophic gastritis with intestinal metaplasia and / or with dysplasia, for reversing the pathology of gastric precancerous lesions, and for preventing the occurrence of gastric cancer, and a treatment method therefor. The product is a lotus leaf extract, and representative active ingredients thereof include, but are not limited to, isoquercetin, quercetin-3-O-glucuronide, hypericin, and nuciferine. The use comprises at least one of treating Helicobacter pylori-negative chronic atrophic gastritis with intestinal metaplasia and / or with dysplasia, reversing gastric precancerous lesions, and preventing the occurrence of gastric cancer. In vitro experiments show that the lotus leaf extract can inhibit malignant proliferation of gastric precancerous lesion epithelial cells and reduce the active oxygen level thereof. In vivo experiments show that the lotus leaf extract can effectively improve the pathology of the Helicobacter pylori-negative gastric precancerous lesions and prevent the occurrence of gastric cancer in animal models. Clinical trials have shown that the lotus leaf extract can effectively treat the pathological conditions and clinical symptoms in patients with the Helicobacter pylori-negative chronic atrophic gastritis with intestinal metaplasia and / or with dysplasia.