229 results about "Myricetin" patented technology

The invention discloses a method used for separating and identifying flavonoid matters in tobacco by adopting liquid chromatography-mass spectrography. The method comprises the following steps: a sample is ground and dried, an extractant is added according to the standard that 0.1 ml of the extractant is added into 1 mg of the sample for extraction and centrifugation, a supernatant liquor is collected, water and chloroform are added according to the standard that 0.5 ml of the water and 1 ml of the chloroform are added into 1 ml of the supernatant liquor for centrifugation, and the supernatant liquor is collected for analysis; 153+ and 151- are adopted as characteristic ions for scanning, parent ions are extracted for second level mass spectrum scanning, and an obtained spectrogram is compared with a spectral library to determine a chemical structure and verified through a reference substance; the molecules and ions of the identified matters and myricetin, dihydromyricetin, catechinic acid / epicatechin, daidzein, glycitein and glycyrrhizin / isoliquiritigenin are adopted as characteristic ions for scanning, the molecules and ions of the searched glycosylation flavonoid matters are used as the parent ions for the second level mass spectrum scanning, an obtained spectrogram is compared with the spectral library to determine a chemical structure and verified through the reference substance. According to the invention, the method is suitable for the analyzing and detecting micro even trace flavonoid matters.

The invention provides an authenticity evaluating method for a populus-type propolis based on multi-index quality control, and caffeic acid, p-coumaric acid, ferulic acid, rutin, myricetin, cinnamic acid, galangin, chrysin, pinocembrin and CAPE in the propolis are detected by high performance liquid chromatography. A mixing standard sample of caffeic acid, p-coumaric acid, ferulic acid, rutin, myricetin, cinnamic acid, galangin, chrysin, pinocembrin and CAPE is taken as a reference index in the method; wherein whether the propolis is from a populus-type material is defined by the existence or inexistence of cinnamic acid, galangin, chrysin, pinocembrin and CAPE, a poplar tree's gum and the propolis are distinguished by the existence or inexistence of caffeic acid, p-coumaric acid and ferulic acid, adulteration or no adulteration of rutin or myricetin is determined by taking rutin or myricetin as a reference; and thus a detection method for distinguishing between the poplar tree's gum and the populus-type propolis, and adulteration or no adulteration of rutin or myricetin, is constructed, and the method helps to evaluate whether a sample is from populus plants, and helps to achieve discarding of the false and retaining of the true about the populous-type propolis.

The invention belongs to the technical field of medicines and relates to a myricetin nanosuspension and a preparation method thereof. The myricetin nanosuspension is prepared by adopting a settling method and a high-pressure homogenization method, and the formula comprises myricetin and a stabilizer according to the weight ratio of 1: 0.25-1: 2.5. According to the preparation method provided by the invention, the formula is optimized, Tween-80, Cremophor EL and D-alpha-tocopheryl polyethylene glycol 1000 succinate (TPGS) are screened as the stabilizer, the prepared myricetin nanosuspension has stable nature and simple composition of the formula, the preparation process is simple, convenient and feasible, and the particle size range is 100nm-300nm; and by reducing the particle size of a medicament, the solubility and the dissolution of the myricetin are obviously improved, and the oral bioavailability and the in-vivo tissue distribution of the myricetin are further improved. In addition, the myricetin nanosuspension can be freeze-dried, and proper excipients are added into the obtained freeze-dried powder for further preparing oral liquid, tablets, granules, capsules and other different oral preparations so as to facilitate clinical applications.

The present invention is directed to extracts of cranberries (Vaccinium macrocarpon) comprising either mixed flavonols that are substantially free of anthocyanins and proanthocyanidins or a purified cranberry flavonol compound, including myricetin-3-β-xylopyranoside, quercetin-3-β-glucoside, quercetin-3-α-arabinopyranoside, 3′-methoxyquercetin-3-α-xylopyranoside, quercetin-3-O-(6″-p-coumaroyl)-β-galactoside, and quercetin-3-O-(6″-benzoyl)-β-galactoside. The present invention also embodies the use of those extracts, as well as extracts comprising the cranberry flavonol compound quercetin-3-α-arabinofuranoside, for the treatment of inflammatory disorders. Pharmaceutical, food, dietary supplement, and cosmetic compositions utilizing the extracts or compounds of the present invention are also recited.

The invention discloses a synthesis method of myricetin. The method includes the steps of: adding an alkaline solution into a dihydromyricetin aqueous solution, and then performing heating under a reflux state; adding a selenium dioxide aqueous solution, then stirring at a reflux temperature for reaction, and then performing cooling to room temperature; adding acid to adjust the pH value, conducting stirring and rotary evaporation, and then performing stirring and filtering; adding an ethanol aqueous solution into a filter cake, performing pulping and filtering, flushing the filter cake with an ethanol aqueous solution, collecting the filter cake, and performing drying to obtain red selenium; combining an organic phases, and performing spin-drying till no ethanol smell, precipitating a large amount of solid, performing filtering, and flushing the filter cake with an ethanol aqueous solution to obtain a yellow solid, i.e. myricetin. The method has the advantages that: 1, the reaction issimple and easy to operate; 2, water and a small amount of ethanol are mainly used as the solvent, and the emission of three wastes is low; 3, red selenium with economic value can be recovered; 4, the obtained myricetin is high in purity; and 5, the method can achieve multiple purposes, has very good economic benefits, and is suitable for large-scale production.

The invention discloses a use of a flavonol compound, which is the use of the flavonol compound in the preparation of antihypoxic medicines, health-care foods or foods. The flavonol compound may be dihydromyricetin, myricetin, dihydroquercetin or quercetin. In the invention, the cell activity test experiment and antihypoxic test of the dihydromyricetin, myricetin, dihydroquercetin and quercetin are performed by adopting a methyl thiazolyl tetrazolium (MTT) method and H9C2 (a rat myocardial cell line). Results show that the four flavonol compounds all have obvious antihypoxic activity and can be used for preparing antihypoxic medicines, health-care products or foods.

The invention discloses epimedium source galactosyl transferase and an application of the epimedium source galactosyl transferase to the preparation of hyperoside, and belongs to the technical field of gene engineering and biological medicines. The invention provides galactosyl transferase and an application of recombinant saccharomyces cerevisiae expressing the galactosyl transferase to the catalytic synthesis of flavonoid compounds. The constructed recombinant saccharomyces cerevisiae can be enabled to catalyze the transfer of UDP-galactose to various flavone substrates such as quercetin, kaempferol, myricetin, dihydroquercetin, dihydrokaempferol, dihydromyricetin, fisetin, morin and icaritin.

The invention provides an HPLC (High Performance Liquid Chromatography) detection method for simultaneously determining 11 kinds of flavonoids, and belongs to the field of flavonoids detection. The 11kinds of flavonoids are delphinidin glucoside, delphinidin rutinoside, peonidin glucoside, cyanidin rutinoside, cyanidin glucoside, rutin, kaempferol, quercetin, myricetin, isorhamnetin and apigenin.According to the HPLC detection method provided by the invention, different kinds of flavonoids matters can be detected within different retention times through accurate limit on an HPLC condition, the minimum separation degree of a chromatographic peak is 1.54, and simultaneous determination of 11 kinds of flavonoids is realized; data of the embodiment shows that simultaneous determination of 11kinds of flavonoids is realized, and the operation is simple and quick; meanwhile, the standard deviation of the peak area and the retention time is smaller, and higher accuracy is realized.