Anti-inflammatory cranberry flavonol extract preparations
a flavonol and extract technology, applied in the field of extracts of cranberries, can solve the problems of reducing the yield of quercetin-3-o-(6′′-benzoyl)--galactoside, causing severe bladder pain, and reducing the yield of quercetin-3-o-(6′′-benzoyl)-galactoside, so as to increase the yield of quercetin-3-o-galactoside and reducing side effects
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example 1
Isolation and Characterization of Flavonol Glycosides and Aglycones from Cranberry Powder
Reagents
[0042] All reagents were purchased from Fisher Scientific (PA) and Sigma (MO) and were of analytical or HPLC grade. Dimethylsulfoxide (DMSO)-d6 (99.96% D) was obtained from Cambridge Isotope Laboratories (MA). Standards of quercetin-3-galactoside, quercetin-3-glucoside, quercetin-3-rhamnoside, quercetin, myricetin and kaempferol were purchased from INDOFINE Chemical Company, Inc. (NJ). Sephadex™ LH-20 for column chromatography was obtained from Amersham Pharmacia Biotech AB (Sweden).
Extraction and Fractionation of Flavonoids
[0043] Flavonoids from 1.0 kg of freeze dried cranberry powder 90-MX supplied by Ocean Spray Cranberries, Inc., (MA) were extracted twice with 80% acetone / water (v / v) (1:10), filtered and partially evaporated under reduced pressure at 35° C. to remove the acetone. The resultant aqueous phase was defatted by extraction with hexane (1:1) and the aqueous layer was ...
example 2
Anti-Inflammatory Activity of Sephadex LH-20 Eluate Fractions
[0076] The anti-inflammatory activities of different classes of cranberry phenolic compounds separated by Sephadex LH-20 column chromatography were tested in vivo with the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse ear edema assay described previously (Huang et al. 2003). Each of the treatments was topically applied to both ears of four female CD-1 mice. Treatments included application of either acetone or curcumin controls or the test extract in 20 μl of acetone twenty minutes prior to application of either 5 mg of acetone alone or 1 nmol of TPA in acetone. At five hours post-treatment, mice were sacrificed and 6 mm diameter ear punch biopsies were taken and weighed. Curcumin, a well-known anti-inflammatory agent, was used as a positive control (Chan et al. 1994; Chan 1995). Data were analyzed statistically using ANOVA and the Student-Neuman-Kuels (SNK) multiple means separation test (P≦0.05). The ratio of ...
example 3
Anti-Inflammatory Activity of Quercetin-3-O-(6″-benzoyl)-β-Galactoside
[0077] The anti-inflammatory activity of quercetin-3-O-(6″-benzoyl)-β-galactoside (Peak 19) was tested in vivo with the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse ear edema assay. Each of the treatments was topically applied to both ears of at least five CD-1 female mice. Treatments included application of either acetone or curcumin controls or the test extract in 20 μl of acetone twenty minutes prior to application of 1.5 nmol of TPA in acetone. After 7 hours post-treatment, mice were sacrificed and 6 mm diameter ear punch biopsies were taken and weighed. The ratio of the difference in the weight of ear punches between TPA treated groups receiving pretreatments of acetone and the test extract, respectively, to the difference in the weight of ear punches between acetone pretreated groups subsequently treated with TPA and acetone, respectively, indicates the degree of anti-inflammatory activity of th...
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