604 results about "Papain" patented technology

The invention relates to a mild exfoliating whitening and repairing body care composition as well as a preparation method and application thereof. The mild exfoliating whitening and repairing body care composition is prepared from the following components in parts by weight: 5 to 10 parts of lipidosome, 0.01 to 1 part of papain and 0.005 to 0.5 part of bromelain, wherein the liposome comprises a shell layer and a core layer, the shell layer comprises lecithin and phytosterol, and the core layer comprises a golden camellia extract and hydrolyzed royal jelly protein; the liposome is prepared from the following raw materials in parts by weight: 2 to 15 parts of lecithin, 0.1 to 5 parts of phytosterol, 0.1 to 3 parts of golden camellia extract and 0.01 to 1 part of hydrolyzed royal jelly protein. The composition can realize multiple effects of brightening skin color, removing waste cutin and strengthening skin, and is safe, mild and non-irritant.

The invention relates to oral liquid for tonifying kidney and bone and a preparation method thereof, and belongs to the technical field of medicine, the oral liquid contains rhizoma drynariae, polygonum multiflorum, poria cocos, teasel root, radix paeoniae alba, angelica sinensis, codonopsis pilosula, prepared rehmannia root, polygonatum kingianum, fructus lycii, native copper and pericarpium citri reticulatae; the rhizoma drynariae is a product obtained by performing enzymolysis on a whole rhizoma drynariae plant through hemicellulase and pectinase, performing enzymolysis through bromelain and papain and performing enzymolysis through nattokinase; polygonum multiflorum is a product obtained by carrying out enzymolysis on Polygonum multiflorum tuberous roots through hemicellulase and pectinase, then carrying out enzymolysis through bromelain and papain and then carrying out enzymolysis through gelatinase. Polygonatum sibiricum is a product obtained by performing enzymolysis on rhizome of Polygonatum sibiricum through hemicellulase and pectinase, performing enzymolysis through bromelain and papain, and performing fermentation through bacillus licheniformis and saccharomyces boulardii. All the active components are matched for use, kidney function metabolism can be well enhanced, bone mineral density is increased, the treatment course is short, the effect taking speed is high, safety and reliability are achieved, and medication compliance is effectively improved.

The invention relates to the field of animal extraction, and provides an earthworm protein extraction method and application thereof in heart and cerebral vessels, the earthworm protein extraction method comprises the following steps: S1, placing living earthworms in a container, adding an ethanol solution, soaking, taking out the earthworms, and cleaning to obtain emetic living earthworms for later use; s2, putting the living earthworm for promoting vomiting into warm water at 30-40 DEG C, adding a protective agent and papain, stirring and soaking, then adding bromelain, stirring and soaking to obtain a mixed solution for later use; s3, grinding the mixed solution, adding modified activated carbon, filtering, taking filtrate 1, and centrifuging to obtain filtrate 2 for later use; and S4, carrying out primary membrane separation on the filtrate 2 to obtain a filtrate 3, carrying out secondary membrane separation, and drying to obtain the earthworm protein. The earthworm protein extraction method provided by the invention has the characteristics of simple operation, low cost, high purity, high activity and high content, and has good commercial application value.

The invention relates to the technical field of fruit wine, in particular to herbal fresh fruit wine and a brewing process thereof. The preparation method comprises the following steps: juicing juicy peaches, apples and raspberries, and adding water for blending; the preparation method comprises the following steps of: adding cellulase and pectinase for primary enzymolysis, adding papain and bromelain for secondary enzymolysis, performing enzyme deactivation and sterilization, inoculating bacillus licheniformis CI CC 24553, lactobacillus plantarum CI CC 21794 and saccharomyces cerevisiae CI CC 1406 for fermentation, and performing post-treatment to prepare the herbal fresh fruit wine. The herbal fresh fruit wine prepared by the invention has the effects of excellent oxidation resistance, high total sugar content and excellent smell and taste, and has better use experience.

The invention relates to the technical field of deep processing of cattle spleen, in particular to cattle spleen active polypeptide capable of enhancing immunity and a preparation method and a functional product thereof. The preparation method comprises the following steps: mincing cattle spleen, adding water, uniformly mixing, heating and curing; adding bacillus licheniformis alkaline protease to carry out hydrolysis for the first time; adding papain and bacillus subtilis flavourzyme into the first hydrolysate to carry out hydrolysis for the second time; and performing enzyme deactivation on the second hydrolysate, and sequentially performing fishy smell removal, decolorization, filtration, concentration and drying treatment to obtain the cattle spleen polypeptide. The cattle spleen polypeptide prepared by the invention can obviously enhance the immune response of a body and play a role in enhancing immunity, can be applied to a functional product for enhancing immunity as a functional factor, has the advantages of low equipment requirement, simple process, easiness in operation, high extraction efficiency and the like, is suitable for industrial production, and has a wide application prospect. The scheme of the invention has good application and economic prospects.

The invention discloses a preparation method of efficient antioxidant sturgeon protein peptide. The preparation method comprises the following steps: S1, extracting a sturgeon skin crude protein solution; s2, extracting sturgeon swimming bladder crude protein; s3, extracting crude protein liquid of the sturgeon meat; s4, performing mixed enzymolysis; and S5, carrying out enzyme deactivation treatment on the obtained enzymatic hydrolysate to obtain enzymatic hydrolysate, and sequentially carrying out ultrafiltration and vacuum drying on the enzymatic hydrolysate to obtain the efficient antioxidant sturgeon protein peptide. Proteins extracted from different parts of sturgeons are mixed according to a specific proportion and then subjected to enzymolysis, the added neutral protease and papain can achieve a synergistic effect, protein is hydrolyzed more comprehensively, small molecule peptides with specific functions are generated, meanwhile, glutathione added during enzymolysis can protect sulfydryl of enzymes, and the enzymolysis effect of the sturgeons is improved. The enzyme activity is maintained, so that the finally obtained sturgeon protein peptide has good oxidation resistance.

The invention relates to an application of sea cucumber intestine polysaccharide in preparation of uric acid lowering drugs. The preparation method of the sea cucumber intestine polysaccharide comprises the following steps: performing enzymolysis treatment on sea cucumber intestines by using papain, performing enzyme deactivation, performing centrifugation, and taking supernate; and carrying out graded alcohol precipitation on the supernate, centrifuging, taking precipitate, deproteinizing and freeze-drying. The preparation method is simple, green and environment-friendly. The sea cucumber intestine polysaccharide prepared by the method can effectively inhibit the activity of xanthine oxidase; the sea cucumber intestine polysaccharide has no toxic effect on HK-2 cells. The compound has a uric acid reducing effect on HK-2 hyperuricemia cells.

The invention provides camel milk active peptide and application thereof in preparation of drugs and edible products for treating and / or preventing diabetes mellitus. Two polypeptide compounds are obtained from papain enzymolysis products of fresh camel milk. Bioactivity evaluation shows that the polypeptide compound has remarkable protein tyrosine phospholipase 1B (PTP1B) inhibitory activity, can enhance insulin receptor sensitivity and promote hepatocyte glucose uptake, has patent medicine potential in the aspect of treating type II diabetes, and can be used for preparing medicines for treating type II diabetes. And a new material basis is provided for developing innovative medicines for treating type II diabetes and developing innovative PTP1B inhibitor hypoglycemic medicines.

The invention discloses camel milk polypeptide with xanthine oxidase inhibitory activity and application thereof.The camel milk polypeptide is prepared by taking fresh camel milk as a raw material, conducting low-temperature centrifugation to remove fat, conducting triple enzymolysis through papain, pepsin and trypsin to obtain enzymatic hydrolysate, enabling the enzymatic hydrolysate to pass through an ultrafiltration membrane (the molecular weight cutoff is 10 kDa) to obtain filtrate, and conducting freeze-drying to obtain the camel milk polypeptide. The compound has xanthine oxidase inhibitory activity. The invention also discloses a small molecule polypeptide with xanthine oxidase inhibitory activity. The filtered solution is screened and combined with polypeptides by adopting a xanthine oxidase affinity ultrafiltration method, the activity of the polypeptides is verified through molecular docking and in-vitro enzyme inhibition, four small-molecule polypeptides with xanthine oxidase inhibitory activity are obtained, the sequence of the small-molecule polypeptides is shown as any one of SEQ ID NO.1-4, and the small-molecule polypeptides can be used for preparing drugs with the effect of inhibiting the uric acid level.

The invention relates to a preparation method of a placenta active factor as well as a product and application of the placenta active factor, and the preparation method comprises the following steps: soaking and rinsing a full-month normal delivery placenta, and homogenizing to obtain homogenate; repeatedly freezing and thawing the homogenate to collect a thaw solution, centrifuging the thaw solution, taking a supernatant, mixing the supernatant with a compound enzyme, and carrying out ultrasonic enzymolysis extraction to obtain an enzymolysis product; and centrifuging the enzymolysis product, taking the supernatant, and carrying out membrane filtration to obtain the placenta active factor. The compound enzyme comprises a combination of animal protein hydrolysis compound enzyme, alkaline protease, neutral protease and papain. The placenta tissue is extracted through cooperation of the specific compound enzyme and ultrasonic waves, the placenta tissue and the specific compound enzyme have a synergistic effect, the extraction efficiency can be further improved, the content of active ingredients in the extract is high, the biological activity is high, and the obvious anti-oxidation and anti-aging effects are achieved.

The invention discloses a temperature-controllable preparation method for tissue temperature dissociation into single cells and application, the temperature-controllable preparation method for tissue temperature dissociation into single cells comprises the following steps: S1, providing a tissue sample, and pre-treating the tissue sample to obtain a sample block; s2, mixing the sample blocks with a compound digestive enzyme, and filtering to obtain a single-cell suspension after digestion treatment is completed; wherein the compound digestive enzyme is prepared from collagenase, hyaluronidase and papain; and selectively, S3, carrying out red removing treatment. The invention not only provides an improved preparation method of the single-cell suspension, but also has higher efficiency on the premise of ensuring the yield and activity of single cells, and provides a new strategy and tool for cancer research and treatment by particularly enriching CSCs.

The invention relates to the technical field of biological extraction, and provides a cattle spleen peptide composition as well as a preparation method and application thereof. The preparation method of the cattle spleen peptide comprises the following steps: S1, taking a cattle spleen, adding normal saline, and homogenizing to obtain first homogenate; s2, adding combined enzyme into the first homogenate, uniformly stirring, and performing first water bath treatment to obtain second homogenate; s3, adding chymotrypsin into the second homogenate, uniformly stirring, and performing second water bath treatment to obtain third homogenate; s4, centrifuging the third homogenate to obtain supernate; s5, performing ultrafiltration and drying on the supernate to obtain the cattle spleen peptide. The combined enzyme comprises subtilisin, bromelain and papain. Through the technical scheme, the problem of low cattle spleen peptide extraction rate in related technologies is solved.

The invention discloses a ganoderma spore full-ingredient separation and utilization method and application, and belongs to the technical field of processing. The method comprises the following steps: S1, preparing emulsion and spore wall shells; s2, performing concentration and alcohol precipitation on the emulsion to obtain supernate and precipitate; s3, respectively drying the supernate and the precipitate to obtain a small molecule peptide component and a polysaccharide component; s4, performing enzymolysis on the spore wall shells by using a compound enzyme A and a compound enzyme B, and performing solid-liquid separation to obtain enzymatic hydrolysate and material residues; the compound enzyme A comprises cellulase and chitinase, and the compound enzyme B comprises papain, bromelain and alpha-amylase; s5, preparing soluble dietary fibers from enzymatic hydrolysate; s6, drying and extracting the residues to obtain ganoderma lucidum spore oil and residues; and S7, preparing chitosan from the residual materials. According to the method, separation of various products can be completed at a time, and full-ingredient separation and utilization of the ganoderma lucidum spores are achieved. Compared with the prior art, the yield of the spore oil and the soluble dietary fiber is higher, and the purity of the soluble dietary fiber can be improved.

The invention discloses a pesticide residue scavenging agent. The feed additive is prepared from the following components in parts by weight: 90-110 parts of white hyacinth beans, 9-11 parts of papain, 90-110 parts of liquorice, 45-55 parts of folium isatidis, 25-35 parts of perilla leaves, 25-35 parts of fructus forsythiae, 25-35 parts of honeysuckle, 15-25 parts of rhizoma smilacis glabrae, 15-25 parts of ginkgo leaves, 4-6 parts of soybean protease, 9-11 parts of glutathione, 5-7 parts of amino-oligosaccharin, 9-11 parts of amylase and 7-9 parts of walnut peptide. The invention also discloses a preparation method of the pesticide residue scavenger. The method has the following outstanding effects: the method is easy to operate, low in cost, popular in price and remarkable in effect, and can reach the national safety agricultural product indexes.

The invention discloses a process method for preparing fish collagen tripeptide and application of the fish collagen tripeptide, and belongs to the technical field of collagen peptide preparation processes. According to the method, the raw material fish skin is subjected to pretreatment and pretreatment, and then the pretreated fish skin is subjected to two times of enzymolysis treatment, so that efficient hydrolysis of fish skin collagen is realized, and a collagen peptide product with small molecular weight is obtained. Firstly, neutral protease and papain are used for carrying out primary enzymolysis on fish skin, then a microbial fermentation agent and an activity accelerant are used for carrying out deep fermentation enzymolysis treatment, peptide fragments can be effectively further cut, the yield of small molecule peptide fragments is increased, and the biological activity is improved. The method is simple, the process is easy to control, environmental pollution is avoided, the enzymolysis time is short, the enzymolysis speed and the extraction rate of the collagen peptide can be increased, and the collagen peptide obtained through the method is low in molecular weight, good in biological activity and easier to popularize and apply.

The invention provides a preparation method, application and composition of oat polypeptide, and relates to the technical field of cosmetics. The preparation method disclosed by the invention comprises the following steps: (1) moistening and swelling oat, drying after steam explosion treatment, and adding pectinase, cellulase and amylase to carry out carbohydrate enzymolysis, so as to obtain oat residues after enzymolysis; (2) adding compound protease into the oat residues subjected to enzymolysis, and carrying out primary proteolysis under the condition that the addition amount of ethanol with the volume concentration of 3-8%, so as to obtain enzymolysis slurry A; the compound protease comprises papain, bromelain and flavourzyme in a mass ratio of (3-6): (3-6): 2; (3) adding trypsin into the enzymolysis serous fluid A to carry out secondary proteolysis to obtain enzymolysis serous fluid B; and (4) carrying out ultrafiltration on the enzymolysis slurry B to obtain the oat polypeptide. The obtained oat polypeptide is small in molecular weight and high in content, is used for preparing cosmetics, and has remarkable effects of preserving moisture, resisting wrinkles and reducing skin roughness.

The invention relates to the field of preparation of sodium hyaluronate, in particular to a preparation method of low-molecular-weight sodium hyaluronate. The method specifically comprises the following steps: stirring and dissolving high-molecular-weight sodium hyaluronate powder into water at 45-60 DEG C to obtain a high-molecular-weight sodium hyaluronate solution; cooling to 35-45 DEG C, adding a buffer solution, uniformly mixing, and then adding papain and hyaluronidase in a mass ratio of (2-3): (3-6) to carry out enzymolysis reaction, so as to obtain enzymatic hydrolysate; adding a sodium hydroxide solution into the enzymatic hydrolysate, adjusting the pH value to 7.5-9.0, heating to 80-90 DEG C, treating for 20-40 minutes, and filtering to obtain a low-molecular-weight sodium hyaluronate solution; and performing spray drying. The preparation method is simple in process, mild in condition and free of damage to the product structure, and the prepared low-molecular-weight sodium hyaluronate is high in purity, uniform in molecular weight distribution and good in permeability.

The invention provides a preparation method of a composite Pickering emulsion based on modified yeast protein. Comprising the following steps: preparing modified yeast protein through combined treatment of papain and TG enzyme; mixing the modified yeast protein solution with a chitosan solution, and heating at 90 DEG C to obtain a proteoglycan composite solution, wherein the mass ratio of yeast protein to chitosan is (5: 1)-(1: 5); after the proteoglycan composite solution is cooled to room temperature, the pH value is adjusted to 2.5-6.5; 20%-60% of vegetable oil is added into the proteoglycan composite solution, and the Pickering emulsion can be obtained through homogenization. The composite Pickering emulsion based on the modified yeast protein is suitable for complex food matrixes, good in stability and capable of efficiently embedding various functional components and remarkably delaying oxidation, photolysis or thermal degradation of the functional components, so that the product quality is improved, and the shelf life of the product is prolonged. If the monascus pigment is added, the problems of color loss and degradation of the monascus pigment in the application process can also be improved.

The invention discloses a method for preparing soybean protein hydrolysate-polysaccharide composite gel based on enzymolysis treatment, and belongs to the technical field of food processing and manufacturing. According to the method for preparing the soybean protein hydrolysate-polysaccharide composite gel based on the enzymolysis treatment, the soybean protein hydrolysate is prepared by performing enzymolysis treatment on the soybean protein through papain, and meanwhile, the polysaccharide is added in an auxiliary manner, so that a theoretical basis is laid for actual production and industrial application of soybean protein products with excellent gel property. The main process comprises the following steps: (1) carrying out extrusion puffing treatment and enzymolysis; (2) spray drying; (3) papain treatment; (4) adding carrageenan; and (5) heating, cooling and molding. The gel property of the soybean protein is effectively improved through the action of the papain and the addition of the polysaccharide, better economic benefits can be created for soybean gel products and the processing industry of the soybean gel products, development of the technical field of food processing and manufacturing is promoted, and remarkable economic and social benefits are achieved.

The invention relates to a preparation method of small-molecular-weight sea cucumber oligopeptide, the prepared sea cucumber oligopeptide and application, and belongs to the technical field of skin care products. The preparation method of the small-molecular-weight sea cucumber oligopeptide comprises the following steps: preparing sea cucumber fine powder; adding the sea cucumber fine powder into ultrapure water, adding papain and EDTA (Ethylene Diamine Tetraacetic Acid), and carrying out enzymolysis under the assistance of microwaves; adding trypsin and calcium ions, and carrying out enzymolysis under the assistance of microwaves; adding neutral protease and zinc ions, and performing enzymolysis under the assistance of microwaves to obtain final sea cucumber enzymatic hydrolysate; and decolorizing, separating and purifying, and freeze-drying to obtain the powdery small-molecular-weight sea cucumber oligopeptide. According to the preparation method of the small-molecular-weight sea cucumber oligopeptide, microwave heating and enzymolysis reaction are combined, the hydrolysis degree of a peptide chain is regulated and controlled by controlling an enzyme hydrolysis sequence and enzymolysis conditions, the small-molecular-weight sea cucumber oligopeptide with relatively concentrated and moderate molecular weight is prepared, and good biological activity of the sea cucumber oligopeptide is kept.

The invention discloses a preparation method of black garlic polysaccharide, which comprises the steps of extraction, enzymolysis deproteinization, ultrafiltration, concentration, drying, crushing and the like, and'enzymolysis deproteinization + ultrafiltration 'is the core step of the method. Wherein the enzymolysis deproteinization can be selected from papain, neutral protease, acid protease, alkaline protease and the like or a combination of the papain, the neutral protease, the acid protease and the alkaline protease. In the ultrafiltration step, an organic ultrafiltration membrane or an inorganic ceramic membrane or a combination of the organic ultrafiltration membrane and the inorganic ceramic membrane can be selected. The method has the remarkable advantages of simple process flow, low cost, safety, high efficiency, good finished product quality and the like. Pharmacological experiments prove that the black garlic polysaccharide prepared by the method disclosed by the invention has a remarkable anti-constipation effect, and can be used for preparing anti-constipation healthy products, including medicines, health-care foods and foods.

The invention discloses a preparation method of a micro-ecological bacterium enzyme preparation and application of the micro-ecological bacterium enzyme preparation in a feed additive, and belongs to the field of preparation of a fermented feed by combining a compound microorganism strain with a biological enzyme. Centrifugally separating and mixing bacterial liquid cultured by the acid-resistant zymophyte to obtain a mixed bacterial precipitate, and freeze-drying the mixed bacterial precipitate to obtain mixed bacterial powder; freezing a mixed enzyme solution formed by mixing papain, bromelain and cellulase to obtain mixed enzyme powder; and mixing the mixed bacterial powder and the mixed enzyme powder to form the micro-ecological bacterial enzyme preparation. Mixing and stirring the micro-ecological bacterium enzyme preparation, basal feed and water, and fermenting to obtain a fermentation mixture; adjusting the pH value of the fermentation mixture with hydrochloric acid, mixing with aldehyde chitosan, stirring and reacting to obtain a cross-linked fermentation product, and drying the cross-linked fermentation product with hot air to obtain the solid fermentation feed.

The invention relates to the technical field of medical care, and particularly discloses a preparation method and application of sea cucumber peptide. The preparation method of the sea cucumber peptide provided by the invention comprises the following steps: heating sea cucumber primary pulp for denaturation, and then adding compound protease for enzymolysis treatment to obtain sea cucumber enzymatic hydrolysate; the addition amount of the compound protease is 0.1-0.15 mg / mL, and the compound protease is prepared from papain, ficin and trypsin according to the weight ratio of 1: (0.5-1.5): (2-3); adding activated carbon and diatomite into the sea cucumber enzymatic hydrolysate, and performing enzyme deactivation, filtration and concentration treatment to obtain sea cucumber peptide; the invention also provides application of the sea cucumber peptide obtained by the preparation method in medicines and foods. According to the preparation method of the sea cucumber peptide, the sea cucumber peptide with small molecular weight and good uniformity can be obtained, and the prepared sea cucumber peptide tablet candy has the effects of relieving physical fatigue, resisting aging, resisting oxidation and the like.

The invention discloses a high-efficiency enzymatic fermentation soy sauce production process, and relates to the technical field of soy sauce production processes, the high-efficiency enzymatic fermentation soy sauce production process comprises the following process steps: S1, material preparation, S2, material steaming, S3, inoculation, S4, fermented grain preparation and fermentation, S5, squeezing, and S6, sterilization and filling. The high-temperature-resistant amylase is added in the material steaming process, starch on the surfaces of the wheat and soybean meal raw materials is preliminarily decomposed, the starchy raw material can be decomposed in advance, growth of the aspergillus oryzae raw material in starter propagation is facilitated, clinker can be prevented from caking and being sticky, and the looseness of the starter material is improved; papain is added in the starter propagation process, and protein in wheat and soybean meal raw materials is continuously decomposed by aspergillus oryzae protease in starter propagation, so that the protein raw materials are quickly decomposed in advance, the growth of the aspergillus oryzae raw materials in starter propagation is facilitated, and the fermentation period is shortened; the compound clarification enzyme is added during fermentation to decompose intermediate products such as phytic acid, cellulose and the like which cause a large amount of aggregation after the raw soy sauce is heated, the clarification speed is high after the soy sauce is sterilized, and the precipitation amount is reduced by 50%-70%.

The invention discloses a preparation method of micromolecular active collagen peptide, which comprises the following steps: (1) cutting fresh leather into fragments, pretreating the leather with a sodium bicarbonate solution, rinsing the leather with deionized water, and refrigerating and storing; (2) mixing papain, bromelain, pancreatin, pepsase, alkaline protease, neutral protease and flavourzyme to obtain a compound enzyme preparation; (3) performing high-pressure steam sterilization on the leather at 110-140 kPa and 120-122 DEG C to obtain leather with the hydrolysis degree of 75-85%, then adding distilled water into the leather to obtain mucilage, then adding a compound enzyme preparation into the mucilage, reacting for 5.5-6.5 hours at the pH of 6.8-7.2 and the temperature of 50-55 DEG C, and then performing enzyme inactivation at the temperature of 85-95 DEG C; and (4) after the reaction system is cooled to room temperature, purifying and centrifuging the reaction system, and taking clarified supernate, thereby obtaining the product. Different animal skins are treated, the proportion of peptide fragments with the molecular weight smaller than 1000 Da of the prepared small-molecule active collagen peptide is larger than or equal to 80%, and the hydroxyproline content is larger than 3%.

The invention relates to the technical field of utilization of traditional Chinese medicinal materials, and discloses an efficient separation and preparation method of an astragalus extract, which comprises the following steps: carrying out supercritical extraction on raw materials, then carrying out water extraction, sequentially adding cellulase and papain in the water extraction process, carrying out enzymolysis treatment, centrifuging an astragalus extracting solution after the water extraction is finished, and collecting supernate to obtain the astragalus extract. And performing ultrafiltration by using ultrafiltration membranes with different molecular weights to obtain extracts with different molecular weights, and finally performing vacuum freeze drying. According to the invention, the extraction process is optimized, effective components are extracted, and the technology is simple in preparation method and wide in raw material source, can be used as raw materials of dietary therapy products, health foods and auxiliary and therapeutic drugs, and has a wide application prospect.

The invention relates to the technical field of food processing, in particular to a chicken premade dish and a preparation method thereof. By innovatively integrating a laccase-chequer-shaped indocalamus leaf cross-linking system and a double-frequency ultrasonic pretreatment process, the water retention, bacteriostasis and flavor regulation performance of the chicken premade dish can be synchronously improved, and the effect cannot be replaced by other enzymes such as papain; besides, double-frequency ultrasonic pretreatment is adopted, dynamic balance of deep penetration-surface tenderization is achieved through frequency alternation, and compared with single-frequency or traditional soaking, the method can better cooperate with laccase catalysis to directionally cross-link the preservative, and the effects of water retention, bacteriostasis and flavor regulation and control are achieved.

A compound having the following structure:wherein: R1 is a linker having the formula —(CH2)n-L1-, wherein L1 contains 1-6 carbon atoms and at least one —NH— linkage and at least one oxygen-containing or sulfur-containing linkage, and n is an integer of 0-3; R2 is a group having the formula —C(Y)-E, wherein Y is O or S, and E is a hydrocarbon group and either: (i) at least one carbon-carbon or carbon-nitrogen unsaturated bond or (ii) at least one alkyl halide group; R3 is selected from H, NR′2, NHC(O)R′, and —(CH2)p-T, wherein T contains at least one —NH— linkage; Ra, Rb, Rc, Rd, Re, and Rf are independently selected from of H, hydrocarbon groups containing 1-3 carbon atoms, fluorine atom, and chlorine atom; X is N or CR9, wherein R9 is selected from H, hydrocarbon groups containing 1-3 carbon atoms, fluorine atom, and chlorine atom; and pharmaceutically acceptable salts thereof.

The invention discloses a processing method of fermented cottonseed meal for replacing soybean meal. The method comprises the following steps: uniformly mixing cottonseed meal, feather meal, corn starch and water to obtain a fermentation substrate, adding a first-stage fermentation inoculant into the fermentation substrate for aerobic fermentation, adding a second-stage fermentation inoculant for anaerobic fermentation after fermentation is finished, and performing low-temperature drying and crushing on a fermentation product after fermentation is finished to obtain the cottonseed meal fermented feed. The first-stage fermentation inoculant comprises candida utilis, aspergillus oryzae, papain and cellulase; and the second-stage fermentation inoculant comprises lactobacillus reuteri and lactobacillus acidophilus. According to the method, a bacterium-enzyme synergistic two-step fermentation mode is adopted, bacterium-enzyme synergistic aerobic fermentation is carried out firstly, then anaerobic fermentation is carried out with compound probiotics, and the obtained cottonseed meal fermented feed is high in crude protein and amino acid content and good in palatability, can completely replace soybean meal, can effectively regulate fat metabolism and can improve the lean meat percentage of fattening pigs.

The invention discloses sheep blood phosphopeptide chelated iron prepared by a one-pot method, a preparation method and application, and relates to the technical field of preparation methods of peptide chelated iron, the method comprises the following steps: dissolving sheep blood powder in water, and preheating to 50-55 DEG C to obtain a sheep blood powder solution; papain is added into the sheep blood powder solution for enzymolysis, a sheep blood peptide solution is obtained, sodium pyrophosphate is added into the sheep blood peptide solution for phosphorylation modification, and a sheep blood phosphopeptide reaction solution is obtained; adjusting the pH value of the sheep blood phosphopeptide reaction liquid to 3.0-4.0 to dissociate heme groups to obtain dissociation liquid; and centrifuging the dissociation liquid, and recovering the supernatant to obtain the sheep blood phosphopeptide chelated iron. In the preparation process, endogenous iron ions contained in the heme are utilized, so that the complex preparation process of the current blood peptide iron chelate is simplified.