306 results about "Papain" patented technology

The invention relates to an application of sea cucumber intestine polysaccharide in preparation of uric acid lowering drugs. The preparation method of the sea cucumber intestine polysaccharide comprises the following steps: performing enzymolysis treatment on sea cucumber intestines by using papain, performing enzyme deactivation, performing centrifugation, and taking supernate; and carrying out graded alcohol precipitation on the supernate, centrifuging, taking precipitate, deproteinizing and freeze-drying. The preparation method is simple, green and environment-friendly. The sea cucumber intestine polysaccharide prepared by the method can effectively inhibit the activity of xanthine oxidase; the sea cucumber intestine polysaccharide has no toxic effect on HK-2 cells. The compound has a uric acid reducing effect on HK-2 hyperuricemia cells.

The invention discloses camel milk polypeptide with xanthine oxidase inhibitory activity and application thereof.The camel milk polypeptide is prepared by taking fresh camel milk as a raw material, conducting low-temperature centrifugation to remove fat, conducting triple enzymolysis through papain, pepsin and trypsin to obtain enzymatic hydrolysate, enabling the enzymatic hydrolysate to pass through an ultrafiltration membrane (the molecular weight cutoff is 10 kDa) to obtain filtrate, and conducting freeze-drying to obtain the camel milk polypeptide. The compound has xanthine oxidase inhibitory activity. The invention also discloses a small molecule polypeptide with xanthine oxidase inhibitory activity. The filtered solution is screened and combined with polypeptides by adopting a xanthine oxidase affinity ultrafiltration method, the activity of the polypeptides is verified through molecular docking and in-vitro enzyme inhibition, four small-molecule polypeptides with xanthine oxidase inhibitory activity are obtained, the sequence of the small-molecule polypeptides is shown as any one of SEQ ID NO.1-4, and the small-molecule polypeptides can be used for preparing drugs with the effect of inhibiting the uric acid level.

The invention provides a preparation method, application and composition of oat polypeptide, and relates to the technical field of cosmetics. The preparation method disclosed by the invention comprises the following steps: (1) moistening and swelling oat, drying after steam explosion treatment, and adding pectinase, cellulase and amylase to carry out carbohydrate enzymolysis, so as to obtain oat residues after enzymolysis; (2) adding compound protease into the oat residues subjected to enzymolysis, and carrying out primary proteolysis under the condition that the addition amount of ethanol with the volume concentration of 3-8%, so as to obtain enzymolysis slurry A; the compound protease comprises papain, bromelain and flavourzyme in a mass ratio of (3-6): (3-6): 2; (3) adding trypsin into the enzymolysis serous fluid A to carry out secondary proteolysis to obtain enzymolysis serous fluid B; and (4) carrying out ultrafiltration on the enzymolysis slurry B to obtain the oat polypeptide. The obtained oat polypeptide is small in molecular weight and high in content, is used for preparing cosmetics, and has remarkable effects of preserving moisture, resisting wrinkles and reducing skin roughness.

The invention provides a preparation method of a composite Pickering emulsion based on modified yeast protein. Comprising the following steps: preparing modified yeast protein through combined treatment of papain and TG enzyme; mixing the modified yeast protein solution with a chitosan solution, and heating at 90 DEG C to obtain a proteoglycan composite solution, wherein the mass ratio of yeast protein to chitosan is (5: 1)-(1: 5); after the proteoglycan composite solution is cooled to room temperature, the pH value is adjusted to 2.5-6.5; 20%-60% of vegetable oil is added into the proteoglycan composite solution, and the Pickering emulsion can be obtained through homogenization. The composite Pickering emulsion based on the modified yeast protein is suitable for complex food matrixes, good in stability and capable of efficiently embedding various functional components and remarkably delaying oxidation, photolysis or thermal degradation of the functional components, so that the product quality is improved, and the shelf life of the product is prolonged. If the monascus pigment is added, the problems of color loss and degradation of the monascus pigment in the application process can also be improved.

The invention discloses a high-efficiency enzymatic fermentation soy sauce production process, and relates to the technical field of soy sauce production processes, the high-efficiency enzymatic fermentation soy sauce production process comprises the following process steps: S1, material preparation, S2, material steaming, S3, inoculation, S4, fermented grain preparation and fermentation, S5, squeezing, and S6, sterilization and filling. The high-temperature-resistant amylase is added in the material steaming process, starch on the surfaces of the wheat and soybean meal raw materials is preliminarily decomposed, the starchy raw material can be decomposed in advance, growth of the aspergillus oryzae raw material in starter propagation is facilitated, clinker can be prevented from caking and being sticky, and the looseness of the starter material is improved; papain is added in the starter propagation process, and protein in wheat and soybean meal raw materials is continuously decomposed by aspergillus oryzae protease in starter propagation, so that the protein raw materials are quickly decomposed in advance, the growth of the aspergillus oryzae raw materials in starter propagation is facilitated, and the fermentation period is shortened; the compound clarification enzyme is added during fermentation to decompose intermediate products such as phytic acid, cellulose and the like which cause a large amount of aggregation after the raw soy sauce is heated, the clarification speed is high after the soy sauce is sterilized, and the precipitation amount is reduced by 50%-70%.

The invention discloses a preparation method of micromolecular active collagen peptide, which comprises the following steps: (1) cutting fresh leather into fragments, pretreating the leather with a sodium bicarbonate solution, rinsing the leather with deionized water, and refrigerating and storing; (2) mixing papain, bromelain, pancreatin, pepsase, alkaline protease, neutral protease and flavourzyme to obtain a compound enzyme preparation; (3) performing high-pressure steam sterilization on the leather at 110-140 kPa and 120-122 DEG C to obtain leather with the hydrolysis degree of 75-85%, then adding distilled water into the leather to obtain mucilage, then adding a compound enzyme preparation into the mucilage, reacting for 5.5-6.5 hours at the pH of 6.8-7.2 and the temperature of 50-55 DEG C, and then performing enzyme inactivation at the temperature of 85-95 DEG C; and (4) after the reaction system is cooled to room temperature, purifying and centrifuging the reaction system, and taking clarified supernate, thereby obtaining the product. Different animal skins are treated, the proportion of peptide fragments with the molecular weight smaller than 1000 Da of the prepared small-molecule active collagen peptide is larger than or equal to 80%, and the hydroxyproline content is larger than 3%.

The invention relates to the field of skincare products, and particularly discloses skincare gel loaded with ganoderma lucidum exosome and a preparation method of the skincare gel. The skin care gel loaded with the lucid ganoderma exosome is prepared from the following raw materials in parts by weight: 20 to 40 parts of hydrogel matrix, 2 to 4 parts of lucid ganoderma exosome nanoparticles, 2 to 6 parts of niobium carbide nanosheets, 1 to 3 parts of nicotinamide, 0.1 to 2 parts of phenethyl resorcinol and 0.1 to 3 parts of green tea extract, the Mailing exosome nanoparticles are of a core-shell structure, a core layer comprises Mailing exosome and papain in a mass ratio of 1: (0.1-0.2), and a shell layer comprises a polylactic acid-glycolic acid copolymer and a penetration enhancer in a mass ratio of 1: (0.1-0.3). The skin-care gel provided by the invention has remarkable whitening, moisturizing and skin brightening effects.

The invention relates to the technical field of microorganisms, in particular to a preparation method of fermented papaya juice and application of the fermented papaya juice. Green papaya left after processing in a papain factory is used as a raw material, raw material matching is optimized, cooked papaya is used and mixed, adaptability of the raw material is screened and optimized, and the fermented papaya juice is obtained. According to the present invention, the strain suitable for papaya juice fermentation and capable of highly producing GABA (gamma-aminobutyric acid) is obtained, the GABA production capacity of the strain is improved through the activation of the medicinal and edible plant liquid, the GABA content of the papaya juice can be further improved, and in addition, the optimal production process and the ratio of the high-yield GABA of the papaya juice are obtained through the orthogonal optimization; besides, the milk beverage is produced by optimizing a fermented papaya juice production process, the technical problem that pure fermented papaya juice is heavy in sour and rotten taste is effectively solved, and the papaya beverage has the aroma and taste characteristics of papaya and milk and is a papaya beverage with a health-care function and good taste.

The invention relates to the technical field of cosmetics, and particularly discloses emulsion type foaming cleaning scrub cream with a skin care effect and a preparation method of the emulsion type foaming cleaning scrub cream. The scrub cream is prepared from the following raw material components in percentage by mass: 8 to 15 percent of glycerol, 0.2 to 0.9 percent of sodium hydroxide, 0.3 to 0.8 percent of citric acid, 0.03 to 0.2 percent of EDTA (Ethylene Diamine Tetraacetic Acid) disodium, 0.5 to 1.5 percent of an auxiliary agent, 1.5 to 3.0 percent of a thickening agent A, 1.5 to 3.0 percent of a thickening agent B, 5.86 to 1457 percent of an emollient, 12 to 22 percent of a surface active foaming agent, 0.8 to 3.0 percent of an emulsifying agent, 2.5 to 13 percent of a cutin stripping agent, 0.7 to 1.3 percent of a preservative, 0.32 to 0.92 percent of a skin conditioning agent and the balance of water, the skin conditioner is a mixture prepared from sodium hyaluronate, tocopherol, papain, nicotinamide, carnosine, panthenol and phytosterol oleate.

The invention relates to the technical field of biological extraction, in particular to a preparation method of selenium polysaccharide in cardamine violifolia, which comprises the following steps: extracting selenium polysaccharide in cardamine violifolia by using a deep eutectic solvent as an extraction solvent and adopting an ultrasonic method, and removing protein in the selenium polysaccharide by using an isoelectric precipitation method and an enzymolysis method, thereby obtaining the selenium polysaccharide in cardamine violifolia. Removing water, enzymolysis product amino acid, inorganic selenium and other impurities by a membrane separation concentration system to obtain cardamine violifolia selenium polysaccharide; wherein the eutectic solvent comprises choline chloride and malonic acid in a molar ratio of 1: 1-1: 3, and the water content is 40-60% (mass percent); the compound enzyme is prepared from bromelain and papain in a mass ratio of 1: 1. Protein in the selenium polysaccharide is removed by adopting deep eutectic solvent ultrasonic extraction and an isoelectric precipitation method combined with an enzymolysis method, enzymolysis product amino acid, inorganic selenium and other impurities are removed by adopting an ultrafiltration membrane separation and concentration system, the structure of the polysaccharide is not damaged, and the high-purity cardamine violifolia selenium polysaccharide is obtained.

The invention discloses a preparation method and application of milk-derived peptide capable of efficiently promoting calcium absorption and bone formation, and relates to the field of dairy product processing. Casein in milk is hydrolyzed through papain or protease of similar enzymolysis sites, a primary product is obtained through concentration, and a final product is obtained through subsequent storage or drying and other technological operations. The obtained hydrolysate has outstanding functions of promoting calcium absorption and bone formation, especially in bone formation.

The present invention discloses a Dendrobium officinale protein hydrolysate composition, a preparation method thereof, and an application thereof in anti-aging cosmetics. The Dendrobium officinale protein hydrolysate composition consists of Dendrobium officinale protein hydrolysates TDM-1 and TDM-5, with a mass ratio of 1:(0.5-2); TDM-1 and TDM-5 are peptide components with a molecular weight ≤10 kDa and a peptide component with a molecular weight of 10 kDa < ≤50 kDa, which are obtained by enzymatic hydrolysis of Dendrobium officinale crude protein using a neutral protease and papain. TDM-1 and TDM-5 components have synergistic effects of antioxidant, anti-inflammatory, and barrier repair by inhibiting the activation of the MAPK / NF-κB signaling pathway and promoting ECM repair mediated by the TGF-β pathway, thereby achieving multi-target anti-aging and having low irritation. They can be used in dosage forms such as essences and creams, providing core raw materials for the development of natural anti-aging cosmetics.

The invention discloses giant salamander grease and application thereof in a blood fat reducing product. The giant salamander grease is obtained by adding papain into giant salamander tissues for enzymolysis and then adsorbing by using an egg white modified microsphere adsorbent. The giant salamander grease is refined by using the egg white modified microsphere adsorbent, and then the giant salamander grease is compounded with the dark tea extract, the hawthorn extract and the lotus leaf extract and processed into embedded particles, so that the water solubility of the giant salamander grease preparation can be improved, and the giant salamander grease preparation can be used as an oral preparation for reducing blood fat.

The invention discloses a method for removing fishy smell of meat through ultrasonic-assisted enzymolysis, and belongs to the technical field of meat processing. The method comprises the following steps: putting a meat raw material into clear water, carrying out ultrasonic treatment for 8-15 minutes, then immersing the treated meat raw material into a compound enzyme solution containing papain and lipase, and carrying out enzymolysis for 30-90 minutes; and soaking the tenderized meat with a lactic acid bacteria fermentation broth for 30-40 min. According to the method, ultrasonic waves are combined with an enzymolysis method, so that the content of fishy smell substances (sulfide, aldehyde and the like) is comprehensively reduced by 53.2%-83.4%, and the score of sensory fishy smell is reduced by 72.4%.

The invention discloses a preparation method of composite active peptide with anti-wrinkle, whitening and freckle-removing effects from marine fish byproducts, cod byproducts, namely fish heads, fish bones and fish skins, are used as raw materials and are mixed according to the mass ratio of 3: 3: 4, and the composite active peptide is prepared through the steps of pretreatment, enzymolysis, enzyme deactivation, centrifugal separation and purification. A compound enzyme used in the enzymolysis step is composed of trypsin and papain according to a certain proportion, and the enzyme addition amount, the enzymolysis time, the enzymolysis temperature and other conditions are optimized. The prepared composite active peptide with the anti-wrinkle, whitening and freckle-removing effects from the marine fish by-product has good anti-wrinkle, whitening and freckle-removing effects, resource utilization of the cod by-product is achieved, and the composite active peptide has high application value.

The invention belongs to the technical field of chitosan oligosaccharide preparation, and particularly relates to a method for preparing chitosan oligosaccharide based on compound enzyme. According to the method for preparing the chitosan oligosaccharide based on the compound enzyme, a multi-enzyme synergistic system is matched with the design of a specific protective agent, chitosanase and chitinase can cooperatively cut beta-1, 4 glycosidic bonds of chitosan and control molecular weight distribution of products, meanwhile, chitinase can directionally cut the beta-1, 4 bonds at the tail end of chitosan, and the molecular weight distribution of the products is controlled. The papain is used for removing protein impurities in the chitosan to realize accurate control of the polymerization degree. Besides, a specific protective agent matched with the multi-enzyme synergistic system is specifically designed, the specific protective agent and the multi-enzyme composition act together, the molecular weight distribution width can be remarkably reduced, the yield and the DP 4-8 proportion can be increased, meanwhile, physical field ultrasonic assistance is added in the enzymolysis treatment process, and the enzymolysis treatment efficiency is improved. The combination of the substrate and the enzyme is further promoted, and the reaction time is shortened.

The invention provides tooth enamel repairing toothpaste and a preparation method thereof, and the tooth enamel repairing toothpaste comprises the following components in percentage by mass: 15-30% of glycerol, 15-30% of glycerol, 5-10% of sodium dodecyl sulfate, 5-10% of sodium dodecyl sulfate, 5-10% of sodium dodecyl sulfate and the balance of deionized water. 10%-25% of hydrated silica; 3%-10% of polyethylene glycol-8; 3%-7% of calcium phosphosilicate; 1%-5% of a surfactant; 0.5%-3% of a binder; 0.05%-0.5% of an antibacterial agent; 0.1%-1.5% of a compound enzyme; the compound enzyme comprises lysozyme, papain and bromelain; the mass ratio of the lysozyme to the papain to the bromelain is 1: (0.4-0.6): (0.4-0.6); and the balance of water. The toothpaste can be used for remarkably improving the repairing effect of enamel and the oral health maintenance capability.

The invention relates to a method for extracting genome DNA of plants rich in secondary metabolites and a buffer solution, and belongs to the technical field of molecular biology and botany. The method solves the technical problems of low DNA extraction efficiency, poor purity, easy degradation and the like when a traditional DNA extraction method is used for treating plant tissues rich in secondary metabolites such as alkaloid, polyphenol and the like. Comprising the following steps: adding a complexing agent such as polyethylene glycol or polyvinylpyrrolidone when grinding plant tissues in a liquid nitrogen environment; splitting by using a cell wall splitting buffer solution containing dithiothreitol and a nonionic surfactant; carrying out DNA release and extraction at 60-70 DEG C by adopting a CTAB (Cetyltrimethyl Ammonium Bromide) extraction buffer solution containing polyethylene glycol and papain; and then purifying and precipitating to obtain high-purity genome DNA (Deoxyribose Nucleic Acid). The method can effectively remove alkaloid, protein and other impurities, significantly improves the DNA yield and purity, and is suitable for genome sequencing, genetic resource protection, medicinal plant molecular identification and the like of plants with high secondary metabolites such as Stephania kwangsiensis and the like.

The invention discloses camellia seed meal oligopeptide as well as a preparation method and application thereof. The method comprises the following steps: S1, pretreating camellia seed meal to obtain a pretreated substance; s2, performing enzymolysis and wall breaking to obtain first enzymatic hydrolysate; wherein the enzymolysis wall breaking adopts a mixed enzyme, and the mixed enzyme comprises amylase and cellulase; s3, extracting crude protein to obtain crude protein; s4, carrying out crude protein enzymolysis on the crude protein to obtain a second enzymatic hydrolysate; wherein the crude proteolysis adopts mixed protease, and the mixed protease comprises first protease and second protease; wherein the first protease is one or more of alkaline protease, neutral protease and acid protease; the second protease is papain; and S5, purifying the second enzymatic hydrolysate to obtain the camellia seed meal oligopeptide. The average molecular weight of the obtained product is smaller than 1000 Daltons, and the product has biological activity and high application value in the fields of cosmetics and functional food.

The invention discloses a hippophae rhamnoides seed meal protein-based hypoglycemic peptide and a preparation method thereof, and the preparation method comprises the following steps: mixing hippophae rhamnoides seed meal powder with water, adjusting the pH value with sodium hydroxide, stirring, and centrifuging to obtain a supernatant; adjusting the pH value of the supernatant with hydrochloric acid, standing, centrifuging, washing the obtained precipitate with distilled water until the precipitate is neutral, pre-freeze-drying, and freeze-drying to obtain SSP powder; the preparation method comprises the following steps: mixing SSP powder with water to obtain an SSP solution, carrying out water bath preheating, adding protease, carrying out enzymolysis, carrying out water bath heating on the solution, adjusting the pH value of the solution to be neutral, collecting a centrifugal supernatant, and carrying out low-temperature dialysis to obtain the hypoglycemic peptide based on the sea buckthorn seed meal protein, wherein the protease comprises alkaline protease, compound protease, neutral protease, bromelain and papain, and the invention aims to excavate the development prospect of the sea-buckthorn seed meal active peptide and expand the application potential of the sea-buckthorn seed meal active peptide in different disease fields.

The invention relates to the technical field of food biology, and discloses an antiviral cubilose polysaccharide active component enriched with bound sialic acid as well as a preparation method and application of the antiviral cubilose polysaccharide active component. The weight-average molecular weight of the cubilose polysaccharide active component is 30-36 kDa, the molecular weight distribution coefficient is 1.1-1.2, the content of bound sialic acid is greater than or equal to 24.0%, and the content of total sugar is greater than or equal to 57.0%. The preparation method comprises the following steps: pretreating a cubilose raw material in a boiling water bath, and sequentially carrying out alkaline protease and neutral protease enzymolysis; carrying out alcohol precipitation on supernate, redissolving, and carrying out papain enzymolysis; and after enzyme deactivation, adjusting the ethanol concentration to 75-85%, precipitating, and drying. According to the method, the cubilose polysaccharide can be efficiently enriched, the combined sialic acid structure of the cubilose polysaccharide is reserved, and the obtained component has the effects of resisting oxidation, inhibiting pathogenic microorganisms and regulating intestinal microecology.

The invention discloses a multi-enzyme compound noodle stretching agent and application thereof, and belongs to the technical field of food additives. The multi-enzyme compound noodle stretching agent is prepared from the following components in parts by mass: 1 to 10 parts of papain, 1 to 10 parts of trypsin, 1 to 10 parts of glucose oxidase, 1 to 10 parts of lipase and 10 to 30 parts of tea polyphenol. Through the synergistic effect of multienzyme compounding and tea polyphenol, the dough structure and the noodle processing performance are improved, the protein depolymerization and crosslinking degree and the protein oxidation degree of the dough can be regulated and controlled at the same time, the dough strength and ductility are considered, the cooking loss is reduced, meanwhile, the green and natural characteristics are kept, no chemical modifier is added, and the production cost is reduced. And the limitation of the existing single enzyme or chemical modifier is overcome, so that the method has a relatively good application prospect in the technical field of food additives.

The invention belongs to the technical field of pet food, and particularly relates to compound papain containing probiotics and application of the compound papain in pet digestion aiding products. The compound papain is prepared from active ingredients and auxiliary ingredients, wherein the active ingredients are mixed with the auxiliary ingredients; the active component is prepared from papain, bacillus subtilis protease and probiotics; the probiotics are prepared from lactobacillus acidophilus and bifidobacterium lactis. According to the compound papain disclosed by the invention, a double-enzyme design of papain and bacillus subtilis protease is adopted, so that the digestibility is remarkably improved, the digestion function of pets is favorably improved, nutrient substances in pet food are favorably absorbed, and the incidence rate of digestive system diseases is further favorably reduced. Besides, the double-probiotic design of lactobacillus acidophilus and bifidobacterium lactis is added on the basis of the double-enzyme design, so that the digestibility of the pet food can be further improved, the defect of adopting single protease or only depending on protease is overcome, and the method has a relatively good application prospect.

The invention discloses modified hydroxytyrosol, flavored hydroxytyrosol, a preparation method of the modified hydroxytyrosol, a preparation method of the flavored hydroxytyrosol, functional food and cosmetics. Activated carbon loaded p-toluenesulfonic acid is used as a catalyst to catalyze papain to modify hydroxytyrosol, so that the effect activity of the modified hydroxytyrosol raw material is reserved, and meanwhile, the bad flavor generated by impurities in industrial production of the hydroxytyrosol raw material is remarkably reduced; furthermore, the smell and the flavor of the hydroxytyrosol raw material are remarkably improved, the application effect of the hydroxytyrosol raw material in related fields is improved, and the use sensory experience of the product is improved. The flavor of hydroxytyrosol is improved in an enzyme preparation catalysis mode, and the process method is green and safe.

The invention provides a cattle stomach preparation method which comprises the following steps and a formula: a compound additive formula: the compound additive formula comprises the following components in percentage by mass: 1-1.5% of papain, 0.3-0.8% of sodium pyrophosphate, 1-2% of baking soda, 0.5-0.8% of sodium carbonate decahydrate, 1.5-2% of salt, 0.1-0.3% of sodium alginate, 0.2-0.4% of sodium tripolyphosphate and 0.3-0.5% of glucose; the preparation method comprises the following steps: (1) soaking in ice water: soaking fresh cattle stomach in water at 4-8 DEG C for 1-2 hours, and adding the composite additive at the same time; (2) cooking: cooking the soaked cattle stomach for 20 minutes by using steam of 85 DEG C; 3) first water permeation: washing the cooked cattle stomach in flowing clear water for 10 minutes to remove impurities; and 4) second water permeation: soaking the cattle stomach in alkalescent water with the pH value of 8.2 for 20 minutes to stabilize the fiber structure. The tenderness is revolutionarily improved, and the product is just melted in the mouth.

The invention relates to the technical field of daily chemicals, in particular to a color-protecting laundry detergent containing plant extracts and a preparation method of the color-protecting laundry detergent. The color-protecting laundry detergent is prepared from the following components in percentage by mass: 12.5 to 18.3 percent of natural anionic surfactant, 6.4 to 9.7 percent of nonionic surfactant, 3.2 to 5.8 percent of plant polyphenol extract, 3.0 to 5.2 percent of fiber affinity natural polymer, 2.1 to 4.0 percent of bio-based chelating aid, 1.8 to 3.5 percent of natural glycoside synergist, 0.9 to 2.3 percent of pH buffering agent, 0.6 to 1.4 percent of antioxidant plant essential oil and the balance of deionized water. The compound protease comprises alkaline protease, neutral protease and papain; the invention aims to solve the technical problems that the existing laundry detergent is poor in color protection effect, insufficient in environmental protection performance and large in damage to fabrics, and provides a laundry detergent product which takes natural plant extracts as core components and has an efficient color protection function.

The invention provides a method for detecting enzyme activity of papain in a hypersensitive protein complex enzyme, which comprises the following steps: mixing the hypersensitive protein complex enzyme with an L-cysteine hydrochloride buffer solution to obtain a sample solution to be detected; adding the to-be-tested sample solution into a substrate solution containing casein, and testing a change curve of a voltage value of the to-be-tested sample solution along with time under the condition that the casein can be decomposed by papain; and determining the enzyme activity of the papain in the hypersensitive protein compound enzyme according to the change curve of the voltage value of the to-be-detected sample solution along with time. The method can solve the problem that the enzyme activity of papain in the hypersensitive protein complex enzyme cannot be accurately detected in the prior art.

The invention belongs to the technical field of protein processing, and particularly relates to modified beta-lactoglobulin as well as a preparation method and application thereof. The modified beta-lactoglobulin is a composite modified product which is obtained by performing a papain hydrolysis treatment process on beta-lactoglobulin to obtain a hydrolysate and then performing glycosylation modification on the hydrolysate by adopting chitosan oligosaccharide and transglutaminase. The product has significantly improved oxidation resistance, emulsifying properties and foaming activity, and is suitable as a food processing ingredient, such as an antioxidant, an emulsifier or a foaming agent.

The invention discloses a preparation method of low-sugar paper food, which is characterized in that sorghum is subjected to curing (baking) and enzymolysis to obtain low-sugar carbon water liquid; performing enzymolysis on soybean protein powder, sodium caseinate and papain, and performing sieving and colloid milling to obtain protein liquid; and adding the protein liquid, the low-sugar carbon water liquid, polyethylene glycol and medium-chain triglyceride, stirring, homogenizing and drying to obtain the paper food with the thickness of 0.1-3mm. The paper sheet food is thin in appearance, good in dissolubility, capable of being dissolved in oral saliva, fine and smooth, capable of being eaten without chewing and low in sugar content.

The application discloses a method for preparing an oat-based beverage through biological enzymolysis and a product thereof, and comprises the following steps: continuously hydrolyzing starch, cellulose, protein and other substances in oat slurry by using high-temperature-resistant amylase, cellulase, saccharifying enzyme, papain and alkaline protease, sufficiently releasing reducing substances in the oat, simultaneously generating reducing peptides, improving the free radical scavenging capacity of the oat slurry, and improving the emulsification stability of the oat slurry due to the moderate degradation of macromolecular substances such as starch, cellulose and protein; glycerol is added in three stages at a controlled speed, so that the glycerol is fully mixed with flaxseed oil, and excessive glycerol is avoided from adhering to lipase to reduce the activity of the enzyme, thereby improving the reaction efficiency.