403 results about "Papain" patented technology

The invention relates to a mild exfoliating whitening and repairing body care composition as well as a preparation method and application thereof. The mild exfoliating whitening and repairing body care composition is prepared from the following components in parts by weight: 5 to 10 parts of lipidosome, 0.01 to 1 part of papain and 0.005 to 0.5 part of bromelain, wherein the liposome comprises a shell layer and a core layer, the shell layer comprises lecithin and phytosterol, and the core layer comprises a golden camellia extract and hydrolyzed royal jelly protein; the liposome is prepared from the following raw materials in parts by weight: 2 to 15 parts of lecithin, 0.1 to 5 parts of phytosterol, 0.1 to 3 parts of golden camellia extract and 0.01 to 1 part of hydrolyzed royal jelly protein. The composition can realize multiple effects of brightening skin color, removing waste cutin and strengthening skin, and is safe, mild and non-irritant.

The invention relates to the technical field of deep processing of cattle spleen, in particular to cattle spleen active polypeptide capable of enhancing immunity and a preparation method and a functional product thereof. The preparation method comprises the following steps: mincing cattle spleen, adding water, uniformly mixing, heating and curing; adding bacillus licheniformis alkaline protease to carry out hydrolysis for the first time; adding papain and bacillus subtilis flavourzyme into the first hydrolysate to carry out hydrolysis for the second time; and performing enzyme deactivation on the second hydrolysate, and sequentially performing fishy smell removal, decolorization, filtration, concentration and drying treatment to obtain the cattle spleen polypeptide. The cattle spleen polypeptide prepared by the invention can obviously enhance the immune response of a body and play a role in enhancing immunity, can be applied to a functional product for enhancing immunity as a functional factor, has the advantages of low equipment requirement, simple process, easiness in operation, high extraction efficiency and the like, is suitable for industrial production, and has a wide application prospect. The scheme of the invention has good application and economic prospects.

The invention relates to an application of sea cucumber intestine polysaccharide in preparation of uric acid lowering drugs. The preparation method of the sea cucumber intestine polysaccharide comprises the following steps: performing enzymolysis treatment on sea cucumber intestines by using papain, performing enzyme deactivation, performing centrifugation, and taking supernate; and carrying out graded alcohol precipitation on the supernate, centrifuging, taking precipitate, deproteinizing and freeze-drying. The preparation method is simple, green and environment-friendly. The sea cucumber intestine polysaccharide prepared by the method can effectively inhibit the activity of xanthine oxidase; the sea cucumber intestine polysaccharide has no toxic effect on HK-2 cells. The compound has a uric acid reducing effect on HK-2 hyperuricemia cells.

The invention discloses camel milk polypeptide with xanthine oxidase inhibitory activity and application thereof.The camel milk polypeptide is prepared by taking fresh camel milk as a raw material, conducting low-temperature centrifugation to remove fat, conducting triple enzymolysis through papain, pepsin and trypsin to obtain enzymatic hydrolysate, enabling the enzymatic hydrolysate to pass through an ultrafiltration membrane (the molecular weight cutoff is 10 kDa) to obtain filtrate, and conducting freeze-drying to obtain the camel milk polypeptide. The compound has xanthine oxidase inhibitory activity. The invention also discloses a small molecule polypeptide with xanthine oxidase inhibitory activity. The filtered solution is screened and combined with polypeptides by adopting a xanthine oxidase affinity ultrafiltration method, the activity of the polypeptides is verified through molecular docking and in-vitro enzyme inhibition, four small-molecule polypeptides with xanthine oxidase inhibitory activity are obtained, the sequence of the small-molecule polypeptides is shown as any one of SEQ ID NO.1-4, and the small-molecule polypeptides can be used for preparing drugs with the effect of inhibiting the uric acid level.

The invention relates to the technical field of biological extraction, and provides a cattle spleen peptide composition as well as a preparation method and application thereof. The preparation method of the cattle spleen peptide comprises the following steps: S1, taking a cattle spleen, adding normal saline, and homogenizing to obtain first homogenate; s2, adding combined enzyme into the first homogenate, uniformly stirring, and performing first water bath treatment to obtain second homogenate; s3, adding chymotrypsin into the second homogenate, uniformly stirring, and performing second water bath treatment to obtain third homogenate; s4, centrifuging the third homogenate to obtain supernate; s5, performing ultrafiltration and drying on the supernate to obtain the cattle spleen peptide. The combined enzyme comprises subtilisin, bromelain and papain. Through the technical scheme, the problem of low cattle spleen peptide extraction rate in related technologies is solved.

The invention provides a preparation method, application and composition of oat polypeptide, and relates to the technical field of cosmetics. The preparation method disclosed by the invention comprises the following steps: (1) moistening and swelling oat, drying after steam explosion treatment, and adding pectinase, cellulase and amylase to carry out carbohydrate enzymolysis, so as to obtain oat residues after enzymolysis; (2) adding compound protease into the oat residues subjected to enzymolysis, and carrying out primary proteolysis under the condition that the addition amount of ethanol with the volume concentration of 3-8%, so as to obtain enzymolysis slurry A; the compound protease comprises papain, bromelain and flavourzyme in a mass ratio of (3-6): (3-6): 2; (3) adding trypsin into the enzymolysis serous fluid A to carry out secondary proteolysis to obtain enzymolysis serous fluid B; and (4) carrying out ultrafiltration on the enzymolysis slurry B to obtain the oat polypeptide. The obtained oat polypeptide is small in molecular weight and high in content, is used for preparing cosmetics, and has remarkable effects of preserving moisture, resisting wrinkles and reducing skin roughness.

The invention relates to the field of preparation of sodium hyaluronate, in particular to a preparation method of low-molecular-weight sodium hyaluronate. The method specifically comprises the following steps: stirring and dissolving high-molecular-weight sodium hyaluronate powder into water at 45-60 DEG C to obtain a high-molecular-weight sodium hyaluronate solution; cooling to 35-45 DEG C, adding a buffer solution, uniformly mixing, and then adding papain and hyaluronidase in a mass ratio of (2-3): (3-6) to carry out enzymolysis reaction, so as to obtain enzymatic hydrolysate; adding a sodium hydroxide solution into the enzymatic hydrolysate, adjusting the pH value to 7.5-9.0, heating to 80-90 DEG C, treating for 20-40 minutes, and filtering to obtain a low-molecular-weight sodium hyaluronate solution; and performing spray drying. The preparation method is simple in process, mild in condition and free of damage to the product structure, and the prepared low-molecular-weight sodium hyaluronate is high in purity, uniform in molecular weight distribution and good in permeability.

The invention provides a preparation method of a composite Pickering emulsion based on modified yeast protein. Comprising the following steps: preparing modified yeast protein through combined treatment of papain and TG enzyme; mixing the modified yeast protein solution with a chitosan solution, and heating at 90 DEG C to obtain a proteoglycan composite solution, wherein the mass ratio of yeast protein to chitosan is (5: 1)-(1: 5); after the proteoglycan composite solution is cooled to room temperature, the pH value is adjusted to 2.5-6.5; 20%-60% of vegetable oil is added into the proteoglycan composite solution, and the Pickering emulsion can be obtained through homogenization. The composite Pickering emulsion based on the modified yeast protein is suitable for complex food matrixes, good in stability and capable of efficiently embedding various functional components and remarkably delaying oxidation, photolysis or thermal degradation of the functional components, so that the product quality is improved, and the shelf life of the product is prolonged. If the monascus pigment is added, the problems of color loss and degradation of the monascus pigment in the application process can also be improved.

The invention discloses a method for preparing soybean protein hydrolysate-polysaccharide composite gel based on enzymolysis treatment, and belongs to the technical field of food processing and manufacturing. According to the method for preparing the soybean protein hydrolysate-polysaccharide composite gel based on the enzymolysis treatment, the soybean protein hydrolysate is prepared by performing enzymolysis treatment on the soybean protein through papain, and meanwhile, the polysaccharide is added in an auxiliary manner, so that a theoretical basis is laid for actual production and industrial application of soybean protein products with excellent gel property. The main process comprises the following steps: (1) carrying out extrusion puffing treatment and enzymolysis; (2) spray drying; (3) papain treatment; (4) adding carrageenan; and (5) heating, cooling and molding. The gel property of the soybean protein is effectively improved through the action of the papain and the addition of the polysaccharide, better economic benefits can be created for soybean gel products and the processing industry of the soybean gel products, development of the technical field of food processing and manufacturing is promoted, and remarkable economic and social benefits are achieved.

The invention relates to a preparation method of small-molecular-weight sea cucumber oligopeptide, the prepared sea cucumber oligopeptide and application, and belongs to the technical field of skin care products. The preparation method of the small-molecular-weight sea cucumber oligopeptide comprises the following steps: preparing sea cucumber fine powder; adding the sea cucumber fine powder into ultrapure water, adding papain and EDTA (Ethylene Diamine Tetraacetic Acid), and carrying out enzymolysis under the assistance of microwaves; adding trypsin and calcium ions, and carrying out enzymolysis under the assistance of microwaves; adding neutral protease and zinc ions, and performing enzymolysis under the assistance of microwaves to obtain final sea cucumber enzymatic hydrolysate; and decolorizing, separating and purifying, and freeze-drying to obtain the powdery small-molecular-weight sea cucumber oligopeptide. According to the preparation method of the small-molecular-weight sea cucumber oligopeptide, microwave heating and enzymolysis reaction are combined, the hydrolysis degree of a peptide chain is regulated and controlled by controlling an enzyme hydrolysis sequence and enzymolysis conditions, the small-molecular-weight sea cucumber oligopeptide with relatively concentrated and moderate molecular weight is prepared, and good biological activity of the sea cucumber oligopeptide is kept.

The invention discloses a preparation method of black garlic polysaccharide, which comprises the steps of extraction, enzymolysis deproteinization, ultrafiltration, concentration, drying, crushing and the like, and'enzymolysis deproteinization + ultrafiltration 'is the core step of the method. Wherein the enzymolysis deproteinization can be selected from papain, neutral protease, acid protease, alkaline protease and the like or a combination of the papain, the neutral protease, the acid protease and the alkaline protease. In the ultrafiltration step, an organic ultrafiltration membrane or an inorganic ceramic membrane or a combination of the organic ultrafiltration membrane and the inorganic ceramic membrane can be selected. The method has the remarkable advantages of simple process flow, low cost, safety, high efficiency, good finished product quality and the like. Pharmacological experiments prove that the black garlic polysaccharide prepared by the method disclosed by the invention has a remarkable anti-constipation effect, and can be used for preparing anti-constipation healthy products, including medicines, health-care foods and foods.

The invention relates to the technical field of medical care, and particularly discloses a preparation method and application of sea cucumber peptide. The preparation method of the sea cucumber peptide provided by the invention comprises the following steps: heating sea cucumber primary pulp for denaturation, and then adding compound protease for enzymolysis treatment to obtain sea cucumber enzymatic hydrolysate; the addition amount of the compound protease is 0.1-0.15 mg / mL, and the compound protease is prepared from papain, ficin and trypsin according to the weight ratio of 1: (0.5-1.5): (2-3); adding activated carbon and diatomite into the sea cucumber enzymatic hydrolysate, and performing enzyme deactivation, filtration and concentration treatment to obtain sea cucumber peptide; the invention also provides application of the sea cucumber peptide obtained by the preparation method in medicines and foods. According to the preparation method of the sea cucumber peptide, the sea cucumber peptide with small molecular weight and good uniformity can be obtained, and the prepared sea cucumber peptide tablet candy has the effects of relieving physical fatigue, resisting aging, resisting oxidation and the like.

The invention discloses a high-efficiency enzymatic fermentation soy sauce production process, and relates to the technical field of soy sauce production processes, the high-efficiency enzymatic fermentation soy sauce production process comprises the following process steps: S1, material preparation, S2, material steaming, S3, inoculation, S4, fermented grain preparation and fermentation, S5, squeezing, and S6, sterilization and filling. The high-temperature-resistant amylase is added in the material steaming process, starch on the surfaces of the wheat and soybean meal raw materials is preliminarily decomposed, the starchy raw material can be decomposed in advance, growth of the aspergillus oryzae raw material in starter propagation is facilitated, clinker can be prevented from caking and being sticky, and the looseness of the starter material is improved; papain is added in the starter propagation process, and protein in wheat and soybean meal raw materials is continuously decomposed by aspergillus oryzae protease in starter propagation, so that the protein raw materials are quickly decomposed in advance, the growth of the aspergillus oryzae raw materials in starter propagation is facilitated, and the fermentation period is shortened; the compound clarification enzyme is added during fermentation to decompose intermediate products such as phytic acid, cellulose and the like which cause a large amount of aggregation after the raw soy sauce is heated, the clarification speed is high after the soy sauce is sterilized, and the precipitation amount is reduced by 50%-70%.

The invention discloses a preparation method of micromolecular active collagen peptide, which comprises the following steps: (1) cutting fresh leather into fragments, pretreating the leather with a sodium bicarbonate solution, rinsing the leather with deionized water, and refrigerating and storing; (2) mixing papain, bromelain, pancreatin, pepsase, alkaline protease, neutral protease and flavourzyme to obtain a compound enzyme preparation; (3) performing high-pressure steam sterilization on the leather at 110-140 kPa and 120-122 DEG C to obtain leather with the hydrolysis degree of 75-85%, then adding distilled water into the leather to obtain mucilage, then adding a compound enzyme preparation into the mucilage, reacting for 5.5-6.5 hours at the pH of 6.8-7.2 and the temperature of 50-55 DEG C, and then performing enzyme inactivation at the temperature of 85-95 DEG C; and (4) after the reaction system is cooled to room temperature, purifying and centrifuging the reaction system, and taking clarified supernate, thereby obtaining the product. Different animal skins are treated, the proportion of peptide fragments with the molecular weight smaller than 1000 Da of the prepared small-molecule active collagen peptide is larger than or equal to 80%, and the hydroxyproline content is larger than 3%.

The invention relates to the technical field of food processing, in particular to a chicken premade dish and a preparation method thereof. By innovatively integrating a laccase-chequer-shaped indocalamus leaf cross-linking system and a double-frequency ultrasonic pretreatment process, the water retention, bacteriostasis and flavor regulation performance of the chicken premade dish can be synchronously improved, and the effect cannot be replaced by other enzymes such as papain; besides, double-frequency ultrasonic pretreatment is adopted, dynamic balance of deep penetration-surface tenderization is achieved through frequency alternation, and compared with single-frequency or traditional soaking, the method can better cooperate with laccase catalysis to directionally cross-link the preservative, and the effects of water retention, bacteriostasis and flavor regulation and control are achieved.

The invention relates to the field of skincare products, and particularly discloses skincare gel loaded with ganoderma lucidum exosome and a preparation method of the skincare gel. The skin care gel loaded with the lucid ganoderma exosome is prepared from the following raw materials in parts by weight: 20 to 40 parts of hydrogel matrix, 2 to 4 parts of lucid ganoderma exosome nanoparticles, 2 to 6 parts of niobium carbide nanosheets, 1 to 3 parts of nicotinamide, 0.1 to 2 parts of phenethyl resorcinol and 0.1 to 3 parts of green tea extract, the Mailing exosome nanoparticles are of a core-shell structure, a core layer comprises Mailing exosome and papain in a mass ratio of 1: (0.1-0.2), and a shell layer comprises a polylactic acid-glycolic acid copolymer and a penetration enhancer in a mass ratio of 1: (0.1-0.3). The skin-care gel provided by the invention has remarkable whitening, moisturizing and skin brightening effects.

The invention provides a cordyceps militaris fermentation extract and application thereof in preservation of agaricus bisporus, and belongs to the technical field of deep processing of edible mushrooms and preservation of agricultural products. The preparation method of the cordyceps militaris fermentation extract comprises the following steps: (1) taking cordyceps militaris sporocarp, adding water, grinding, and performing enzymolysis by using cellulase and papain in sequence to obtain cordyceps militaris pulp; (2) adding glucose and waste beer yeast powder into the cordyceps militaris pulp, then inoculating lactobacillus paracasei and bifidobacterium bifidum, and fermenting; and (3) after fermentation is completed, carrying out ultrasonic crushing, centrifuging to remove thalli, and carrying out spray drying to obtain the cordyceps militaris fermentation extract. The method can effectively reduce the browning index and weight loss ratio of agaricus bisporus, can slow down the increase of malonaldehyde, reduces the activity of PPO enzyme, and prevents enzymatic browning caused by the synergistic effect of the PPO enzyme and peroxidase. The SOD enzyme activity in the agaricus bisporus can be kept, active oxygen in tissues can be removed, and the metabolic balance of the active oxygen can be maintained.

The invention relates to the technical field of microorganisms, in particular to a preparation method of fermented papaya juice and application of the fermented papaya juice. Green papaya left after processing in a papain factory is used as a raw material, raw material matching is optimized, cooked papaya is used and mixed, adaptability of the raw material is screened and optimized, and the fermented papaya juice is obtained. According to the present invention, the strain suitable for papaya juice fermentation and capable of highly producing GABA (gamma-aminobutyric acid) is obtained, the GABA production capacity of the strain is improved through the activation of the medicinal and edible plant liquid, the GABA content of the papaya juice can be further improved, and in addition, the optimal production process and the ratio of the high-yield GABA of the papaya juice are obtained through the orthogonal optimization; besides, the milk beverage is produced by optimizing a fermented papaya juice production process, the technical problem that pure fermented papaya juice is heavy in sour and rotten taste is effectively solved, and the papaya beverage has the aroma and taste characteristics of papaya and milk and is a papaya beverage with a health-care function and good taste.

The invention relates to the technical field of cosmetics, and particularly discloses emulsion type foaming cleaning scrub cream with a skin care effect and a preparation method of the emulsion type foaming cleaning scrub cream. The scrub cream is prepared from the following raw material components in percentage by mass: 8 to 15 percent of glycerol, 0.2 to 0.9 percent of sodium hydroxide, 0.3 to 0.8 percent of citric acid, 0.03 to 0.2 percent of EDTA (Ethylene Diamine Tetraacetic Acid) disodium, 0.5 to 1.5 percent of an auxiliary agent, 1.5 to 3.0 percent of a thickening agent A, 1.5 to 3.0 percent of a thickening agent B, 5.86 to 1457 percent of an emollient, 12 to 22 percent of a surface active foaming agent, 0.8 to 3.0 percent of an emulsifying agent, 2.5 to 13 percent of a cutin stripping agent, 0.7 to 1.3 percent of a preservative, 0.32 to 0.92 percent of a skin conditioning agent and the balance of water, the skin conditioner is a mixture prepared from sodium hyaluronate, tocopherol, papain, nicotinamide, carnosine, panthenol and phytosterol oleate.

The utility model relates to the technical field of seasoning production equipment, in particular to a composite enzymolysis device for extracting chicken essence concentrate, which comprises an enzymolysis tank, a feeding table is fixedly welded at the top of the right side of the enzymolysis tank, a colloid mill is fixedly mounted on the top surface of the feeding table through bolts, and the left side of the colloid mill is communicated with the enzymolysis tank. A first enzymolysis bin and a second enzymolysis bin are arranged at the top end and the bottom end in the enzymolysis tank respectively, a heat preservation shell is fixedly welded to the outer surface of the enzymolysis tank in a surrounding mode, and an electric heating coil is installed between the heat preservation shell and the enzymolysis tank in a surrounding mode. According to the composite enzymolysis device for extracting the chicken essence concentrated solution, the colloid mill is directly additionally arranged and connected to the feeding port of the reaction tank, chicken bone and meat materials are directly conveyed, manual transferring and feeding do not need to be continued, the escalator provides an up-and-down path, the enzymolysis tank is convenient to operate and maintain, the interior of the tank body is divided into two parts, and the operation is convenient. Papain and trypsin can be respectively input to carry out targeted enzymolysis reaction on chicken and chicken aggregate, so that the enzymolysis reaction efficiency is improved.

The invention provides an oxidoreductase composition as well as a preparation method and application thereof, and belongs to the technical field of biology. The composition contains various complex enzymes, acetyl hexapeptide-8, a centella asiatica extract, an aesculus chinensis extract, ceramide NP and other components. The centella asiatica extract is obtained by performing common enzymolysis on centella asiatica through cellulase, pectinase and papain, then performing fermentation through lactobacillus plantarum and bifidobacterium adolescentis and performing purification; the aesculus chinensis extract is obtained by fermenting mature aesculus chinensis seeds with lactobacillus reuteri and lactobacillus bulgaricus and purifying. All the components are matched for use, and meanwhile, the composition has the effects of resisting sugar, diminishing swelling, resisting aging, tightening and smoothing skin, brightening and dissolving fat, and is safe and efficient.

The invention relates to a preparation method, a product and application of a whitening and antioxidant litchi peel extract. The preparation method comprises the following steps: (1) mixing litchi peel powder with water, and carrying out ultrasonic extraction to obtain a litchi peel crude extract; (2) mixing the litchi peel crude extract with a compound enzyme, carrying out an enzymatic hydrolysis reaction, and carrying out enzyme deactivation treatment after the reaction to obtain an enzymatic hydrolysate; (3) performing cation exchange resin purification on the enzymatic hydrolysate to obtain a litchi pericarp extract; the compound enzyme in the step (2) comprises papain, bromelain and neutral protease. The litchi pericarp extract prepared by the invention has excellent antioxidant capacity and whitening effect, is safe and non-irritant, and is more beneficial to absorption by the human skin surface.

The invention relates to the technical field of biological extraction, in particular to a preparation method of selenium polysaccharide in cardamine violifolia, which comprises the following steps: extracting selenium polysaccharide in cardamine violifolia by using a deep eutectic solvent as an extraction solvent and adopting an ultrasonic method, and removing protein in the selenium polysaccharide by using an isoelectric precipitation method and an enzymolysis method, thereby obtaining the selenium polysaccharide in cardamine violifolia. Removing water, enzymolysis product amino acid, inorganic selenium and other impurities by a membrane separation concentration system to obtain cardamine violifolia selenium polysaccharide; wherein the eutectic solvent comprises choline chloride and malonic acid in a molar ratio of 1: 1-1: 3, and the water content is 40-60% (mass percent); the compound enzyme is prepared from bromelain and papain in a mass ratio of 1: 1. Protein in the selenium polysaccharide is removed by adopting deep eutectic solvent ultrasonic extraction and an isoelectric precipitation method combined with an enzymolysis method, enzymolysis product amino acid, inorganic selenium and other impurities are removed by adopting an ultrafiltration membrane separation and concentration system, the structure of the polysaccharide is not damaged, and the high-purity cardamine violifolia selenium polysaccharide is obtained.

The invention discloses a preparation method and application of milk-derived peptide capable of efficiently promoting calcium absorption and bone formation, and relates to the field of dairy product processing. Casein in milk is hydrolyzed through papain or protease of similar enzymolysis sites, a primary product is obtained through concentration, and a final product is obtained through subsequent storage or drying and other technological operations. The obtained hydrolysate has outstanding functions of promoting calcium absorption and bone formation, especially in bone formation.

The invention discloses a preparation method of a dairy cow placenta extract CP1 and application of the dairy cow placenta extract CP1 as an antioxidant substance. The preparation method comprises the following steps: taking normal delivery healthy Chinese Holstein dairy cow placenta homogenate as a substrate, shearing the dairy cow placenta into pieces, homogenizing by adopting a tissue homogenizer, adding papain, and acting for 4.7 hours under the conditions that the pH is 6.5 and the temperature is 55 DEG C; the antioxidative peptide has the advantages that the antioxidative peptide with five potential types is screened by the aid of virtual screening processes after the antioxidative peptide is inactivated in boiling water bath for 10 minutes, affinity of the antioxidative peptide with Keap1 after molecular docking is higher than that of TX6, the affinity of ANNGKQWAEVF with Keap1 is the strongest, accordingly, the antioxidative peptide is marked as CP1, the CP1 is excellent in free radical scavenging capacity, lipid peroxidation inhibition capacity, transition metal chelating capacity and reducing power, and the antioxidative peptide with the antioxidative peptide can be used for preparing antioxidative peptide products. In addition, a Keap1 / Nrf2 pathway in oxidative stress cells can be activated, so that the oxidative stress of the cells is relieved, and the compound can be applied as an antioxidant substance.

The present invention discloses a Dendrobium officinale protein hydrolysate composition, a preparation method thereof, and an application thereof in anti-aging cosmetics. The Dendrobium officinale protein hydrolysate composition consists of Dendrobium officinale protein hydrolysates TDM-1 and TDM-5, with a mass ratio of 1:(0.5-2); TDM-1 and TDM-5 are peptide components with a molecular weight ≤10 kDa and a peptide component with a molecular weight of 10 kDa < ≤50 kDa, which are obtained by enzymatic hydrolysis of Dendrobium officinale crude protein using a neutral protease and papain. TDM-1 and TDM-5 components have synergistic effects of antioxidant, anti-inflammatory, and barrier repair by inhibiting the activation of the MAPK / NF-κB signaling pathway and promoting ECM repair mediated by the TGF-β pathway, thereby achieving multi-target anti-aging and having low irritation. They can be used in dosage forms such as essences and creams, providing core raw materials for the development of natural anti-aging cosmetics.

The invention discloses giant salamander grease and application thereof in a blood fat reducing product. The giant salamander grease is obtained by adding papain into giant salamander tissues for enzymolysis and then adsorbing by using an egg white modified microsphere adsorbent. The giant salamander grease is refined by using the egg white modified microsphere adsorbent, and then the giant salamander grease is compounded with the dark tea extract, the hawthorn extract and the lotus leaf extract and processed into embedded particles, so that the water solubility of the giant salamander grease preparation can be improved, and the giant salamander grease preparation can be used as an oral preparation for reducing blood fat.

The present invention discloses a preparation method of antioxidant sturgeon protein peptides, comprising the following steps: S1, extraction of crude sturgeon skin protein solution; S2, extraction of crude sturgeon swim bladder protein; S3, extraction of crude sturgeon meat protein solution; S4, mixed enzymatic hydrolysis; S5, subjecting the obtained enzymatic hydrolysis product to enzyme inactivation treatment first to obtain an enzymatic hydrolysis solution, and then sequentially ultrafiltering and vacuum drying the enzymatic hydrolysis solution to obtain antioxidant sturgeon protein peptides. The present invention mixes the proteins extracted from different parts of sturgeon in a specific ratio, and then performs enzymatic hydrolysis. The neutral protease and papain added can act synergistically to more comprehensively hydrolyze proteins, generating small peptides with specific functions. At the same time, glutathione added during enzymatic hydrolysis can protect the sulfhydryl groups of the enzyme and maintain the enzyme activity, so that the finally obtained sturgeon protein peptides have good antioxidant properties.

The invention discloses a method for removing fishy smell of meat through ultrasonic-assisted enzymolysis, and belongs to the technical field of meat processing. The method comprises the following steps: putting a meat raw material into clear water, carrying out ultrasonic treatment for 8-15 minutes, then immersing the treated meat raw material into a compound enzyme solution containing papain and lipase, and carrying out enzymolysis for 30-90 minutes; and soaking the tenderized meat with a lactic acid bacteria fermentation broth for 30-40 min. According to the method, ultrasonic waves are combined with an enzymolysis method, so that the content of fishy smell substances (sulfide, aldehyde and the like) is comprehensively reduced by 53.2%-83.4%, and the score of sensory fishy smell is reduced by 72.4%.

The invention discloses a preparation method of composite active peptide with anti-wrinkle, whitening and freckle-removing effects from marine fish byproducts, cod byproducts, namely fish heads, fish bones and fish skins, are used as raw materials and are mixed according to the mass ratio of 3: 3: 4, and the composite active peptide is prepared through the steps of pretreatment, enzymolysis, enzyme deactivation, centrifugal separation and purification. A compound enzyme used in the enzymolysis step is composed of trypsin and papain according to a certain proportion, and the enzyme addition amount, the enzymolysis time, the enzymolysis temperature and other conditions are optimized. The prepared composite active peptide with the anti-wrinkle, whitening and freckle-removing effects from the marine fish by-product has good anti-wrinkle, whitening and freckle-removing effects, resource utilization of the cod by-product is achieved, and the composite active peptide has high application value.