Preparation of functional feed protein dried porcine saluble
A technology of intestinal membrane protein powder and feed protein, which is applied in animal feed, animal feed, application and other directions, can solve the problems that the hydrolysis rate is not greatly improved, the hydrolysis efficiency is affected, and the hydrolysis efficiency is not high, etc. Digestion and absorption rate, the effect of solving protein feed shortage
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Embodiment 1
[0020] 1. Raw materials and reagents
[0021] (1) Porcine intestinal mucosa and leftovers after extracting heparin from pig intestinal mucosa.
[0022] (2) Enzyme preparations: neutral protease, measured activity 100,000 u / g, Guangbo Nanning Pangbo Bioengineering Co., Ltd.; papain, measured activity 190,000 u / g, Guangxi Nanning Pangbo Bioengineering Co., Ltd.
[0023] (3) Wheat bran: finely pulverize the wheat bran, pass through a 60-mesh sieve, and set aside.
[0024] 2. Preparation method
[0025] (1) Pre-treatment of raw materials: Pig intestinal mucosa and waste intestinal mucosa after heparin extraction from pig intestinal mucosa are filtered to remove impurities, dried in an oven at 80°C, crushed dried raw materials, passed through a 60-mesh sieve, and added distilled water to make a bottom A solution with a concentration of 6% is determined to contain 6g of crude protein in 100ml of the solution.
[0026] (2) Add protease for hydrolysis: 1) Add neutral protease to th...
Embodiment 2
[0030] 1. Raw materials and reagents
[0031] (2) Enzyme preparation: neutral protease, papain, alkaline protease, wherein: neutral protease, papain are the same as embodiment 1, alkaline protease: measured activity 200,000 u / g, Guangbo Nanning Pangbo Biological Engineering Co., Ltd. .
[0032] All the other are with embodiment 1.
[0033] 2. Preparation method
[0034] (1) raw material pretreatment: with embodiment 1.
[0035] (2) Add protease for hydrolysis: 1) Add neutral protease to the solution, hydrolyze for 3 hours at substrate concentration of 6%, enzyme dosage 3000U / g protein, pH value 6.5, temperature 50°C; 2) Enzyme inactivation: Temperature 90°C, time 15min; 3) Then add papain, hydrolyze for 3 hours under the conditions of substrate concentration 6%, enzyme dosage 2000U / g protein, pH value 6.5, temperature 50°C; 4) Enzyme inactivation: temperature 90°C , time 15min; 5) Add alkaline protease at the end, under the conditions of substrate concentration 6%, enzyme ...
Embodiment 3
[0038] 1. Raw materials and reagents
[0039] (2) Enzyme preparations: papain, neutral protease.
[0040] All the other are with embodiment 1.
[0041] 2. Preparation method
[0042] (1) raw material pretreatment: with embodiment 1.
[0043] (2) Add protease for hydrolysis: 1) Add papain, hydrolyze for 5 hours under the conditions of substrate concentration 6%, enzyme dosage 3000U / g protein, pH value 7.0, temperature 40°C; 2) Enzyme inactivation: temperature 90°C, The time is 15 minutes; 3) Add neutral protease under the conditions of substrate concentration 6%, enzyme dosage 4000U / g protein, pH value 7.0, temperature 40°C for 5 hours to make enzymatic hydrolysis solution.
[0044] All the other are the same as embodiment 1, make color golden, the denser granular intestinal membrane protein powder of fragrance.
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