Preparation of functional feed protein dried porcine saluble

A technology of intestinal membrane protein powder and feed protein, which is applied in animal feed, animal feed, application and other directions, can solve the problems that the hydrolysis rate is not greatly improved, the hydrolysis efficiency is affected, and the hydrolysis efficiency is not high, etc. Digestion and absorption rate, the effect of solving protein feed shortage

Inactive Publication Date: 2008-07-02
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the protease hydrolysis step, there are two commonly used methods, one is to use a single enzyme for hydrolysis, because protease has a high degree of specificity in the action of peptide bonds, when using a single enzyme hydrolysis, only a few fixed amino acid sites hydrolysis, so that the hydrolysis efficiency is not high; the other is the method of simultaneous enzymolysis with multiple enzymes in different proportions, the method of simultaneous enzymolysis with multiple enzymes is better than the hydrolysis rate of single-enzyme hydrolysis, and can save hydrolysis time, but Pro

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Raw materials and reagents

[0021] (1) Porcine intestinal mucosa and leftovers after extracting heparin from pig intestinal mucosa.

[0022] (2) Enzyme preparations: neutral protease, measured activity 100,000 u / g, Guangbo Nanning Pangbo Bioengineering Co., Ltd.; papain, measured activity 190,000 u / g, Guangxi Nanning Pangbo Bioengineering Co., Ltd.

[0023] (3) Wheat bran: finely pulverize the wheat bran, pass through a 60-mesh sieve, and set aside.

[0024] 2. Preparation method

[0025] (1) Pre-treatment of raw materials: Pig intestinal mucosa and waste intestinal mucosa after heparin extraction from pig intestinal mucosa are filtered to remove impurities, dried in an oven at 80°C, crushed dried raw materials, passed through a 60-mesh sieve, and added distilled water to make a bottom A solution with a concentration of 6% is determined to contain 6g of crude protein in 100ml of the solution.

[0026] (2) Add protease for hydrolysis: 1) Add neutral protease to th...

Embodiment 2

[0030] 1. Raw materials and reagents

[0031] (2) Enzyme preparation: neutral protease, papain, alkaline protease, wherein: neutral protease, papain are the same as embodiment 1, alkaline protease: measured activity 200,000 u / g, Guangbo Nanning Pangbo Biological Engineering Co., Ltd. .

[0032] All the other are with embodiment 1.

[0033] 2. Preparation method

[0034] (1) raw material pretreatment: with embodiment 1.

[0035] (2) Add protease for hydrolysis: 1) Add neutral protease to the solution, hydrolyze for 3 hours at substrate concentration of 6%, enzyme dosage 3000U / g protein, pH value 6.5, temperature 50°C; 2) Enzyme inactivation: Temperature 90°C, time 15min; 3) Then add papain, hydrolyze for 3 hours under the conditions of substrate concentration 6%, enzyme dosage 2000U / g protein, pH value 6.5, temperature 50°C; 4) Enzyme inactivation: temperature 90°C , time 15min; 5) Add alkaline protease at the end, under the conditions of substrate concentration 6%, enzyme ...

Embodiment 3

[0038] 1. Raw materials and reagents

[0039] (2) Enzyme preparations: papain, neutral protease.

[0040] All the other are with embodiment 1.

[0041] 2. Preparation method

[0042] (1) raw material pretreatment: with embodiment 1.

[0043] (2) Add protease for hydrolysis: 1) Add papain, hydrolyze for 5 hours under the conditions of substrate concentration 6%, enzyme dosage 3000U / g protein, pH value 7.0, temperature 40°C; 2) Enzyme inactivation: temperature 90°C, The time is 15 minutes; 3) Add neutral protease under the conditions of substrate concentration 6%, enzyme dosage 4000U / g protein, pH value 7.0, temperature 40°C for 5 hours to make enzymatic hydrolysis solution.

[0044] All the other are the same as embodiment 1, make color golden, the denser granular intestinal membrane protein powder of fragrance.

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Abstract

The invention discloses a method for preparing functionality feed protein intestinal membrane albumen powder, which solves the problems that simultaneous hydrolyzing of multienzyme can affect the hydrolyzing degree and the acquiring rate of the protein peptide, more particularly, the lower acquiring rate of small peptide in the existing intestinal membrane albumen powder preparing technique. The technical scheme includes (1) treating before materials; (2) adding protease to hydrolyze; (3) adding a carrier; (4) preparing through sponging drying; wherein, in the step of hydrolyze the protease, hydrolyzing is carried through on any two of neutral protease, pawpaw protease and alkali protease by adopting a multienzyme grading hydrolyzing method. The functionality feed protein intestinal membrane albumen powder prepared by the method has the obvious advantages in the aspects of the acquiring rate of the protein peptide and the acquiring rate of the small peptide. The invention mixed to feed with other feeds can remarkably improve the performance trait of immature animals.

Description

technical field [0001] The invention relates to an animal protein feed raw material in the technical field of animal husbandry and feed, in particular to a preparation method of functional feed protein intestinal membrane protein powder. Background technique [0002] Dried Porcine Solubles (DPS for short) is a new type of functional nutritional animal protein raw material, derived from pig small intestine and intestinal mucosa, rich in protein, amino acid, oligopeptide, calcium, phosphorus, cellulose and other The functionality of some macro and trace elements, especially DPS, is reflected in its main component-peptide protein. The small peptides (2-6 peptides) contained in the peptide protein have high-efficiency absorption characteristics and special functional properties, which improve animal health. The utilization rate of protein by the body enhances the anti-stress ability of the animal body, so as to achieve the purpose of improving the production performance of the a...

Claims

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Application Information

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IPC IPC(8): A23K1/10A23K1/165
Inventor 王春维侯永清舒夏娃丁斌鹰朱惠玲李建文房桂兵刘昌杜
Owner WUHAN POLYTECHNIC UNIVERSITY
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