78 results about "Proteinase activity" patented technology

The invention belongs to the technical field of biological medicine, and relates to an oral peptide drug delivery system based on a soybean trypsin inhibitor and core-shell hydrogel, the oral peptide drug delivery system comprises therapeutic polypeptide, KTI and a core-shell structure gel matrix, and the problems that an existing oral peptide delivery system is passively protected and drug burst release is remarkable are solved. A chitosan / sodium tripolyphosphate ionic cross-linked network forms an inner core, a sodium alginate / calcium ion'egg box 'structure cross-linked network forms a shell, and therapeutic polypeptides such as liraglutide and KTI are jointly embedded. Through the pH response characteristic and sequential release design of the core-shell structure, KTI is preferentially and rapidly released in the intestinal environment to inhibit local protease activity, then therapeutic polypeptide is slowly released, and active protection and synergistic interaction are achieved. The invention further provides a specific preparation method and parameters. The polypeptide has the advantages of stable structure in a gastric acid environment, targeted release in intestinal tracts, high encapsulation efficiency, capability of avoiding burst release and the like, and provides a new strategy for efficient delivery of oral polypeptide drugs.

According to the invention, lactobacillus rhamnosus UP180 with relatively strong cellulase activity and lactobacillus paracasei ID001 with relatively strong protease activity are combined to prepare composite probiotic powder, the composite probiotic powder is used for jointly fermenting the endothelium corneum gigeriae galli formula, and meanwhile, a polypeptide capable of improving the digestive enzyme activity of Caco-2 cells is found from a fermentation product. The compound strain fermentation effectively decomposes macromolecular proteins in the endothelium corneum gigeriae galli formula and converts the macromolecular proteins into small molecular peptides, effective active ingredients in the formula are fully released, and the conversion effect is obviously superior to that of single strain fermentation. Besides, the endothelium corneum gigeriae galli composition leavening prepared by the invention can improve nutrient absorption of the rats by improving enzyme activity and metal ion chelation in intestinal tracts of the rats in the weaning period and promote growth of the rats in the weaning period, and has a potential application prospect in the field of nutrient absorption of children.

The invention discloses an EST protease activity detection method which comprises the following steps: a, setting an EST experimental group, a negative control group and a blank control group; b, carrying out incubation reaction on the EST experimental group, the negative control group and the blank control group to obtain respective reaction mixtures; c, detecting the influence of the reaction mixture on breast cancer cell proliferation to obtain the proliferation rate of each group; and d, calculating the enzymatic activity of the EST protein. The method disclosed by the invention is simple and easy to implement, good in repeatability and capable of effectively detecting the activity of the EST protease.

Protease activity can be assessed using a microfluidic system that comprises a microfluidic device and solid support, wherein the solid support comprises a protease fusion protein bound to a first surface of the solid support; wherein the protease fusion protein comprises a protease domain, a protease activation sequence, and a fluorescent protein tag, wherein the fluorescent protein tag is bound to the protease domain and to the first surface of the solid support. Protease activity can be assessed in a microfluidic system by reacting an activating protease with a protease fusion protein; wherein the protease fusion protein comprises a protease domain and a protease activation sequence; wherein the activating protease cleaves the protease activation sequence, thereby activating the protease domain in the protease fusion protein to produce an activated protease fusion protein; reacting the activated protease fusion protein with a detectable substrate, thereby generating a detectable signal; and detecting the detectable signal, thereby detecting protease activity in the microfluidic system.

Use of glycogen synthase kinase-3 (GSK3) as a protease, and a GSK3-based proteolysis-targeting chimera (PROTAC) and a preparation method and use thereof are provided, belonging to the technical field of proteolysis. The GSK3 and an N-terminal domain, an intermediate domain, or a C-terminal domain thereof have a protease activity and can achieve efficient proteolysis. Based on the GSK3 and other similar proteases, PROTACs can be developed to achieve efficient targeted proteolysis of a target protein.

The invention relates to methods and compositions for preventing or treating a neuropathology in a subject associated with or induced or caused by a P. gingivalis infection, preventing the deposition of or reducing the level of P. gingivalis gingipain in neuronal tissue, delaying the onset of a P. gingivalis-induced or associated neuropathology, for preventing or slowing the rate of abnormal protein deposition in the neuronal tissue, and / or reducing neuroinflammation, the methods comprising administering an RNA polynucleotide encoding a protein comprising or consisting of: - one or more amino acid sequences of an active site of an Arg- or Lys-gingipain of P. gingivalis, or a sequence that is at least 80% identical thereto; and / or - the amino acid sequence of one or more adhesin binding motifs (ABMs) of an adhesin domain of an Arg- or Lys-gingipain of P. gingivalis, or a sequence that is at least 80% identical thereto, wherein the polynucleotide is capable of being translated in a mammalian cell.

This invention relates to a vulnerary multifunctional composition, based on unripe coffee cherries (peel, mucilage, beans and silverskin), seed extract of Trigonella foenum-graecum, with one or more tissue-inducing molecules derived from the secondary metabolites of both plants. The unexpected and surprising ability of this new composition to solve the problems of chronic non-healing wounds addresses a long felt unmet need in the medical arts. Through a multifaceted approach, it combats microbial infections, reduces chronic inflammation, counters oxidative stress, enhances immune responses, promotes angiogenesis, regulates protease activity, stimulates collagen deposition, increases growth factor availability, facilitates cell migration, and prevents persistent infections. These novel integrated therapies represent a breakthrough in addressing macroscopic wound healing while targeting the underlying microscale mechanisms. This invention also relates to methods for preparing, using, and administering the multifunctional composition for timely healing in mammals with various skin lesions.

This disclosure provides several ribonuclease R variants and their preparation methods. Compared to the parent enzyme, the variants described in this disclosure exhibit increased protease activity per unit and show promising prospects for industrial applications. Furthermore, this disclosure also provides the application of ribonuclease R variants in linear RNA degradation.

The invention discloses a tannic acid-assisted antifouling coating for an inner seawater system as well as a preparation method and application of the tannic acid-assisted antifouling coating. According to the invention, tannic acid is used as an anchoring part of the antifouling coating to play a good fixing role on the surface of a base material through the adsorption effect, and then a physical interval is formed between tannic acid and the active center of protease through the connection of a hydrophilic substance in the middle layer, so that the conformation change or active site blockage possibly caused by tannic acid to protease is reduced, and the anti-fouling effect of tannic acid is improved. The activity of the protease is better maintained, and meanwhile, enough sites are provided for the protease, so that the protease can be effectively fixed on the surface of a base material to form an antifouling coating. Protein components in a biofilm matrix can be effectively degraded due to existence of protease on the surface of the antifouling coating, so that adhesion of bacteria and algae is prevented, and the antifouling coating has excellent antibacterial and anti-algae performance. The preparation method is simple, convenient, rapid, low in cost and suitable for preparation of the protective coating of the marine equipment and facilities in special scenes.

This invention relates to a Bacillus velezensis strain and mutants thereof with surprisingly advantageous properties for a beneficial cleaning effect. These Bacillus strains are particularly good enzyme producers with high protease activity, in particular protease activity and cellulase activity, preferably protease, cellulase, and amylase activity. The protease activity is in particular secreted protease activity. The invention further relates to preparations of these Bacillus strains, a cleaning formulation (F) comprising the Bacillus strains according to the invention, a method for cleaning an object (O) or the human or animal body (B) as well as the use of the cleaning formulation (F) in cleaning an object (O) or the human or animal body (B). It further relates to a feed or food (E) comprising the Bacillus strains according to the invention and a method of feeding an animal.

We disclose a composition, comprising a compound, comprising a melanin precursor that spontaneously synthesizes a melanin when in free form in vivo, and a peptide directly covalently bonded to or indirectly linked to the melanin precursor, wherein the direct covalent bond or indirect link of the peptide to the melanin precursor blocks spontaneous synthesis of the melanin in vivo in the absence of protease activity. We also disclose methods of tumor imaging, tumor detection, diagnosis, and treatment, including methods comprising administering, to a patient suffering from a tumor, the composition; and either surgically resecting the tumor or thermally ablating the tumor, wherein the surgical resection is guided at least in part by contrast imparted by, or the thermally ablating targets cells containing, the melanin, wherein the melanin is spontaneously synthesized in the tumor after administering the composition.

The present application relates to anti-complement Cls antibodies and uses thereof. The present disclosure provides antibodies that bind complement Cls protein; and nucleic acid molecules encoding such antibodies. The present disclosure also provides compositions comprising such antibodies, and methods of making and using such antibodies, nucleic acid molecules, and compositions. The present disclosure provides an isolated, humanized monoclonal antibody that inhibits cleavage of complement component G4, wherein the antibody does not inhibit cleavage of complement component C2. In some cases, the antibody inhibits a component of the classical complement pathway, in some cases the classical complement pathway component is Cls. In some cases, the antibody does not inhibit protease activity of Cls.

The present application relates to a freshness indicator protein related to quality traits of low-temperature stored Siniperca chuatsi, endogenous proteases and applications. The freshness indicator endogenous proteases are related to postmortem proteolysis and meat softening of Siniperca chuatsi, including cathepsin B, cathepsin L and calpain. The freshness indicator proteins are related to quality changes occurring during postmortem cold storage of Siniperca chuatsi, including myoactin 1, myosin binding protein, LDB protein, actin-related protein, etc. The present application determines the change rule of endogenous protease activity of Siniperca chuatsi at different storage stages, and the dynamic influence on muscle proteolysis and muscle degradation of Siniperca chuatsi, and reveals the internal relationship with fish meat sensory quality and processing characteristics, and clearly defines the "critical point" of quality deterioration of Siniperca chuatsi. It provides clear guidance for the control of endogenous enzyme-mediated quality deterioration in the processing and storage process of Siniperca chuatsi.

The invention relates to the technical field of cosmetics, in particular to a blue copper peptide-containing composition and application thereof in cosmetics. The composition comprises the following components in parts by mass: 1 part of blue copper peptide; 0.5 to 5 parts of tetrahydromethylpyrimidine carboxylic acid; 0.05 to 0.5 part of sodium hyaluronate; and 0.05 to 0.5 part of beta-glucan. Preferably, the composition further comprises allantoin and lactobionic acid, based on 1 part by mass of the blue copper peptide, the content of the allantoin is 0.1-0.5 part, the content of the lactobionic acid is 0.2-1.0 part, and the mass ratio of the allantoin to the lactobionic acid is 1: (2-4). In-vitro efficacy experiments prove that the specific combination and proportion can generate a remarkable synergistic effect, and meanwhile, the capabilities of inhibiting the activity of elastinase and promoting epidermal cell repair are improved, so that the excellent dual effects of wrinkle resistance and skin barrier repair are realized. The composition can be used for preparing cosmetics such as essence, emulsion and face cream.

The application belongs to the technical field of biosensing, and particularly relates to a giant magnetoresistance biosensor for detecting protease, a preparation method and application thereof, which comprises a giant magnetoresistance sensor, a glass sheet plated with a gold film, a functional polypeptide and metal ion chelated magnetic beads; wherein the glass sheet plated with the gold film is fixed on the giant magnetoresistance sensor, one end of a peptide chain of the functional polypeptide is connected with the glass sheet plated with the gold film, and the other end is connected with the metal ion chelated magnetic beads; the giant magnetoresistance biosensor provided by the application can realize monitoring of protease activity, has high sensitivity, strong anti-interference ability, stable performance, and is easy to integrate, so that the giant magnetoresistance biosensor can develop into a portable detection device for large-scale promotion, and has wide application prospects in the fields of diagnosis, treatment and related drug development of major diseases.

The application belongs to the field of food preservation technology, and relates to a chitosan-gelatin composite preservative solution based on plasma-activated water modification and a preparation method and application thereof. First, PAW is used to prepare chitosan solution and gelatin solution respectively, and the composite preservative solution is obtained after mixing and homogenizing. The coating is dehydrated to form a preservative film. The PAW is prepared by treating deionized water with low-temperature plasma, and is rich in active ingredients such as hydroxyl radicals and hydrogen peroxide, which can effectively modify chitosan and gelatin, enhance intermolecular crosslinking, and form a composite film with a dense network structure. The composite film can be applied to fish preservation through coating or immersion, can effectively inhibit the growth of microorganisms, and can improve the color of fish fillets. In addition, the composite film has the function of delaying protein degradation by inhibiting endogenous protease activity, thereby maintaining the muscle tissue structure and prolonging the shelf life of chilled fish fillets. The application has the advantages of simple process, green safety, remarkable preservation effect, and wide market application prospect.

The invention relates to application of an MI-2 compound in preparation of a medicine for treating acute myelogenous leukemia, and belongs to the technical field of biological medicine. It is proved for the first time that in AML cells without MALT1 protease activity, the compound MI-2 (MALT1 inhibitor) can inhibit cell proliferation and induce apoptosis through a brand new mechanism independent of MALT1 inhibition, namely specific regulation and control of lipid metabolism of the AML cells, and therefore the compound MI-2 (MALT1 inhibitor) serves as a candidate drug or a brand new treatment strategy for treating acute myelogenous leukemia for the first time.

Disclosed herein are polypeptides that are useful for, among other things, inhibiting or suppressing the activity of a kallikrein-related protease and therefore treating diseases and conditions involving dysregulated kallikrein-related protease activity. Pharmaceutical compositions and therapeutic methods using the polypeptides and pharmaceutical compositions are also provided.

The present invention relates to mammalian cells modified to have reduced AEP protease activity. Such cells can be used for producing recombinant proteins.

The invention relates to the technical field of marine technology and enzyme, and particularly discloses alkaline serine protease and application thereof. The brand-new alkaline serine protease is successfully mined by using a marine-derived microbial metagenome database, the limitation of conventional microbial isolated culture is overcome, and the source selection range of the alkaline serine protease is greatly widened. Moreover, the alkaline serine protease has excellent protease activity and detergent tolerance, and can be effectively used for protein degradation or used as a detergent component.

The invention relates to the technical field of biological medicine, in particular to a medicine for targeting cathepsin B and application of the medicine in autism treatment. The medicine is prepared into an oral or injection preparation by synthesizing a small-molecule inhibitor with high selectivity and combining with pharmaceutic adjuvants, so that the activity of cathepsin B can be effectively inhibited, neuroinflammation can be relieved, and synaptic function balance can be recovered. Experiments show that the medicine remarkably improves social behavior defects and repetitive behaviors of BTBR T + Itpr3tf / J mice, and meanwhile, the activity of cathepsin B and the level of inflammatory factors in brain tissue are reduced. The dosage of the composition is 0.1-10 milligrams per kilogram of body weight, the administration route comprises oral administration or injection, and the storage condition is that the composition is stored in a dark place at the temperature of 2-8 DEG C. The invention provides a clear action mechanism and a remarkable treatment effect.

This invention relates to a Bacillus velezensis strain and mutants thereof with surprisingly advantageous properties for a beneficial cleaning effect. These Bacillus strains are particularly good enzyme producers with high protease activity, in particular protease activity and cellulase activity, preferably protease, cellulase, and amylase activity. The protease activity is in particular secreted protease activity. The invention further relates to preparations of these Bacillus strains, a cleaning formulation (F) comprising the Bacillus strains according to the invention, a method for cleaning an object (O) or the human or animal body (B) as well as the use of the cleaning formulation (F) in cleaning an object (O) or the human or animal body (B). It further relates to a feed or food (E) comprising the Bacillus strains according to the invention and a method of feeding an animal.

The invention relates to the technical field of cosmetics, in particular to a firming and relieving composition and application thereof.The skin care composition is prepared from peach resin extract, inonotus obliquus extract, purslane extract, sodium hyaluronate cross-linked polymer and porphyridium cruentum extract. The composition obtained by compounding the raw materials can significantly inhibit the activity of elastinase, has a certain inhibition effect on the activity of hyaluronidase, has significant tightening and soothing effects, and is mild and non-irritating.

The objective is to provide an active ingredient for topical skin preparations or food and beverages that is derived from natural products, has excellent biocompatibility and stability, and further possesses excellent skin penetration and metabolic properties. [Solution] A topical skin preparation or food / beverage containing a fish scale extract solution or a fermented product of fish scales by microorganisms as an active ingredient, characterized in that the microorganisms have protease activity or aminopeptidase activity.

The present invention relates to animal feed or animal feed additives comprising polypeptides having protease activity and uses thereof. It also relates to the methods for producing the proteases and for using the proteases to improve animal performance and the nutritional value of an animal feed.

This invention discloses a strain of Aspergillus oryzae AspG1 and its application in Pu-erh tea fermentation, belonging to the field of microbial strain resource development and deep processing technology of tea. The method provided by this invention includes: mixing sun-dried Pu-erh tea raw materials with water at a 1:1 ratio, sterilizing to obtain a solid tea base, inoculating with AspG1 strain, and then carrying out solid-state fermentation under staged temperature control conditions. Experiments showed that the fermentation product exhibited sweet and fruity aroma characteristics on day 14, and purified to floral aroma on day 21; the protease activity of AspG1 strain was increased by 229.42% compared with the control, and the β-glucosidase activity was increased by 58.21%; gas chromatography-mass spectrometry analysis showed that the cis-linalool OAV value in the AspG1 fermentation product reached 275.41 (approximately 31.8 times higher than the control), and 1-octen-3-one was specifically detected (OAV reached 228967.43). This invention provides microbial resources and process solutions for the targeted regulation of aroma in the solid-state fermentation of Pu-erh tea.

The invention discloses a beta-lactoglobulin nanofiber as an adjuvant for oral delivery of therapeutic polypeptide drugs and application of the beta-lactoglobulin nanofiber, and belongs to the technical field of drug delivery adjuvants, the nanofiber is formed by heat treatment of BLG under an acidic condition, and has good gastrointestinal tract stability and severe environment tolerance; in the oral administration process, the nanofibers can temporarily induce the activity of calcium-dependent calpain to be improved, so that the intestinal epithelial permeability is enhanced, and the absorption of polypeptide drugs is facilitated; the rapid recovery of the intestinal barrier function can also be promoted. Insulin is loaded in an enteric capsule coated with 3% of sodium alginate, and by utilizing the BLG nanofiber mediated intestinal barrier regulation effect, compared with subcutaneous injection, the oral administration of insulin shows that the area under a blood glucose curve (AUC) is obviously reduced in a mouse model, and the blood glucose AUC is reduced by about 20.8%. The nanofiber is high in safety and meets the clinical large-scale production requirement.

Methods and devices for capturing aerosolized organic biomaterials including proteases in exhaled air using packed bed columns to enable rapid, low-cost detection of several diseases including lower respiratory tract infections (LRTIs). Methods and devices for examining proteolysis activity using a substrate-based probe or a plurality of substrate based probes and MALDI-TOFMS to detect LRTIs. Sequential methods including examining proteolysis activity of the captured proteases to predict the presence of LRTI followed by a qPCR assay, may not only predict or confirm the presence of LRTI, but may also provide pathogen-specific information, and offers a comprehensive solution for managing LRTI cases.

A method of preventing or reducing hairball formation or promoting hairball elimination in an animal, the method comprising administering to the animal a composition comprising a polypeptide having protease activity.