130 results about "Proteinase activity" patented technology

The invention discloses enterobacter hormaechei XW-01 and a microbial preparation thereof, the strain (preservation number is CCTCC NO: M 20242595) can efficiently degrade protein, total sugar and nicotine in tobacco leaves, sensory indexes such as aroma and aroma quantity are remarkably improved, and protease activity is remarkably improved when the enterobacter hormaechei XW-01 and wheat bran act synergistically. The microbial preparation is obtained through the processes of strain activation, fermentation culture and microbial agent preparation, and when the microbial preparation is applied to tobacco leaf treatment, through the steps of water content adjustment, preparation spraying, sealed fermentation, low-temperature drying and the like, the technical breakthrough that the protein content is reduced, the tar release amount is reduced, and the comprehensive quality grade is improved is achieved. Compared with a traditional method, the technology has the advantages that the treatment period is shortened, the cost is greatly reduced, the COD value of wastewater is greatly reduced, the cigarette quality and the production environmental protection property are remarkably improved, and the method has a wide industrial application prospect.

The invention discloses an anti-aging composition and a preparation method thereof, and belongs to the technical field of anti-aging of cosmetics. The anti-aging composition is prepared from the following components in percentage by mass: 0.2 to 0.6 percent of roxburgh rose peptide, 1.1 to 1.3 percent of hydrolyzed abelmoschus esculentus extract, 0.7 to 0.9 percent of ligusticum wallichii root extract, 1.5 to 1.7 percent of silybum marianum seed oil and the balance of auxiliary materials, the roxburgh rose peptide is prepared by a two-step enzymolysis method, and the hydrolyzed abelmoschus esculentus extract is prepared by a two-step enzymolysis method. The ligusticum wallichii root extract is obtained through supercritical CO2 extraction. Performance tests show that the composition can significantly inhibit the activity of elastase, the inhibition rate reaches 70% or above, the composition has excellent DPPH free radical scavenging capacity, the scavenging rate reaches 80% or above, all the components are synergistic, skin aging can be effectively delayed, and the composition has good application prospects.

The invention relates to the technical field of microbiology and food processing, in particular to pediococcus acidilactici and application of the pediococcus acidilactici in preparation of a brain-intestinal axis regulating efficacy metagen product.The high-activity pediococcus acidilactici DY-1 is separated out from natural fermentation pickles of super-polyselenium plant cardamine violifolia for the first time; the strain can be widely applied to fermentation of high-selenium plant-based food, can significantly promote glycolysis release of selenium-containing active substances, has good acid resistance, high protease activity and high lipase activity, can fully ferment plant-based fermentation raw materials, and has high yield. Glycolysis of macromolecular substances such as protein, saccharides and fat is facilitated, accumulation of active ingredients such as essential amino acid, unsaturated fatty acid, alkaloid and phenolic substances is promoted, and the edibility and functionality of the product are improved; and the organic selenium-rich metagen product prepared by sterilizing the fermentation mixture can synergistically improve human body cognition and emotion regulation regulated by microorganism-intestinal-brain axis.

The invention provides a method for preparing a protease beverage by fermenting cyperus esculentus powder and a product thereof. Comprising the steps of directional pretreatment, fermentation medium preparation, segmented fermentation and optional blending, specifically, directional pretreatment adopts low-temperature extrusion at the temperature of 60-70 DEG C and ultrasonic treatment at the power of 200-300 W for cooperative treatment, and the fiber structure and antinutritional factors of soybean meal are effectively broken; in the fermentation stage, lactobacillus plantarum, saccharomyces cerevisiae and bacillus coagulans composite flora are inoculated, anaerobic fermentation is performed at the temperature of 35-37 DEG C, the pH value of the system is reduced to 4.0-4.5, and then L-cysteine hydrochloride-vitamin B synergist is added for secondary fermentation in a micro-aerobic environment at the temperature of 38-40 DEG C. The fermentation process provided by the invention is simple, the treatment is convenient, the used equipment is conventional equipment in the food industry, and the method is suitable for large-scale industrial production. Meanwhile, in the prepared fermentation liquor, the protease activity is larger than or equal to 320 U / mL, the total number of viable bacteria is larger than or equal to 1.2 * 10 CFU / mL, the optimum pH of the protease is 3.8-4.8, the optimum temperature is 37-39 DEG C, the protease is highly matched with the human gastrointestinal tract environment, and the method has wide industrialization prospects.

The invention provides a pet hair decomposer with synergistic effect of bacillus subtilis and keratinase, the pet hair decomposer comprises bacillus subtilis ZYpet-015 and keratinase, the preservation number of the bacillus subtilis ZYpet-015 is CGMCC (China General Microbiological Culture Collection Center) No.34790, and the preservation date is June 6, 2025; the protease activity of the bacillus subtilis is greater than or equal to 129.92 U, the survival rate of the bacillus subtilis in simulated gastric juice within 3 hours is greater than 50%, and the tolerance rate of the bacillus subtilis in intestinal juice within 4 hours is greater than 90%. According to the pet hair decomposing agent, hair keratin is synergistically degraded through protease secreted by the bacillus subtilis ZYpet-015 and keratinase, and the hair decomposing efficiency reaches 28.07% + / -2.79% and is remarkably higher than that of a single treatment group. Meanwhile, experiments prove that the pet hair decomposer can effectively destroy the hair cuticle structure, is suitable for a pet hair-melting nutritional supplement and can relieve the hair ball symptom.

The invention belongs to the technical field of biological medicine, and relates to an oral peptide drug delivery system based on a soybean trypsin inhibitor and core-shell hydrogel, the oral peptide drug delivery system comprises therapeutic polypeptide, KTI and a core-shell structure gel matrix, and the problems that an existing oral peptide delivery system is passively protected and drug burst release is remarkable are solved. A chitosan / sodium tripolyphosphate ionic cross-linked network forms an inner core, a sodium alginate / calcium ion'egg box 'structure cross-linked network forms a shell, and therapeutic polypeptides such as liraglutide and KTI are jointly embedded. Through the pH response characteristic and sequential release design of the core-shell structure, KTI is preferentially and rapidly released in the intestinal environment to inhibit local protease activity, then therapeutic polypeptide is slowly released, and active protection and synergistic interaction are achieved. The invention further provides a specific preparation method and parameters. The polypeptide has the advantages of stable structure in a gastric acid environment, targeted release in intestinal tracts, high encapsulation efficiency, capability of avoiding burst release and the like, and provides a new strategy for efficient delivery of oral polypeptide drugs.

According to the invention, lactobacillus rhamnosus UP180 with relatively strong cellulase activity and lactobacillus paracasei ID001 with relatively strong protease activity are combined to prepare composite probiotic powder, the composite probiotic powder is used for jointly fermenting the endothelium corneum gigeriae galli formula, and meanwhile, a polypeptide capable of improving the digestive enzyme activity of Caco-2 cells is found from a fermentation product. The compound strain fermentation effectively decomposes macromolecular proteins in the endothelium corneum gigeriae galli formula and converts the macromolecular proteins into small molecular peptides, effective active ingredients in the formula are fully released, and the conversion effect is obviously superior to that of single strain fermentation. Besides, the endothelium corneum gigeriae galli composition leavening prepared by the invention can improve nutrient absorption of the rats by improving enzyme activity and metal ion chelation in intestinal tracts of the rats in the weaning period and promote growth of the rats in the weaning period, and has a potential application prospect in the field of nutrient absorption of children.

The invention discloses an EST protease activity detection method which comprises the following steps: a, setting an EST experimental group, a negative control group and a blank control group; b, carrying out incubation reaction on the EST experimental group, the negative control group and the blank control group to obtain respective reaction mixtures; c, detecting the influence of the reaction mixture on breast cancer cell proliferation to obtain the proliferation rate of each group; and d, calculating the enzymatic activity of the EST protein. The method disclosed by the invention is simple and easy to implement, good in repeatability and capable of effectively detecting the activity of the EST protease.

Protease activity can be assessed using a microfluidic system that comprises a microfluidic device and solid support, wherein the solid support comprises a protease fusion protein bound to a first surface of the solid support; wherein the protease fusion protein comprises a protease domain, a protease activation sequence, and a fluorescent protein tag, wherein the fluorescent protein tag is bound to the protease domain and to the first surface of the solid support. Protease activity can be assessed in a microfluidic system by reacting an activating protease with a protease fusion protein; wherein the protease fusion protein comprises a protease domain and a protease activation sequence; wherein the activating protease cleaves the protease activation sequence, thereby activating the protease domain in the protease fusion protein to produce an activated protease fusion protein; reacting the activated protease fusion protein with a detectable substrate, thereby generating a detectable signal; and detecting the detectable signal, thereby detecting protease activity in the microfluidic system.

The invention relates to the technical field of protein extraction, in particular to a high-content earthworm protein extraction method and device.The extraction method comprises the following steps that S1, a fluid-state protein protective agent and a granular-state protein protective agent are sequentially added into smashed live earthworms, and mixed powder is obtained; s2, water is added into the mixed powder for centrifugation, and supernate is obtained; s3, performing enzymolysis and filtration on the supernate; s4, the filtrate is subjected to activated carbon adsorption and freeze drying, and earthworm protein is obtained; the equipment sequentially comprises a pretreatment chamber, a centrifugal cylinder, an enzymolysis adsorption chamber and a freeze-drying chamber from left to right, according to the extraction method, the protein protective agent is immediately added after the earthworms are crushed, and protein degradation is prevented and protein integrity and biological activity are maintained by inhibiting endogenous protease activity and chelating metal ions; and the protein protective agent maintains the structural stability of the substrate, so that the subsequent enzymolysis is more sufficient, and the protein yield is improved.

Embodiments provided herein provide polypeptides and molecules, including polypeptides and variant Fc molecules, with protease activity, pharmaceutical compositions comprising same, and methods that can be used to treat disorders, such as IgG-related disorders.

Use of glycogen synthase kinase-3 (GSK3) as a protease, and a GSK3-based proteolysis-targeting chimera (PROTAC) and a preparation method and use thereof are provided, belonging to the technical field of proteolysis. The GSK3 and an N-terminal domain, an intermediate domain, or a C-terminal domain thereof have a protease activity and can achieve efficient proteolysis. Based on the GSK3 and other similar proteases, PROTACs can be developed to achieve efficient targeted proteolysis of a target protein.

The invention relates to methods and compositions for preventing or treating a neuropathology in a subject associated with or induced or caused by a P. gingivalis infection, preventing the deposition of or reducing the level of P. gingivalis gingipain in neuronal tissue, delaying the onset of a P. gingivalis-induced or associated neuropathology, for preventing or slowing the rate of abnormal protein deposition in the neuronal tissue, and / or reducing neuroinflammation, the methods comprising administering an RNA polynucleotide encoding a protein comprising or consisting of: - one or more amino acid sequences of an active site of an Arg- or Lys-gingipain of P. gingivalis, or a sequence that is at least 80% identical thereto; and / or - the amino acid sequence of one or more adhesin binding motifs (ABMs) of an adhesin domain of an Arg- or Lys-gingipain of P. gingivalis, or a sequence that is at least 80% identical thereto, wherein the polynucleotide is capable of being translated in a mammalian cell.

This invention relates to a vulnerary multifunctional composition, based on unripe coffee cherries (peel, mucilage, beans and silverskin), seed extract of Trigonella foenum-graecum, with one or more tissue-inducing molecules derived from the secondary metabolites of both plants. The unexpected and surprising ability of this new composition to solve the problems of chronic non-healing wounds addresses a long felt unmet need in the medical arts. Through a multifaceted approach, it combats microbial infections, reduces chronic inflammation, counters oxidative stress, enhances immune responses, promotes angiogenesis, regulates protease activity, stimulates collagen deposition, increases growth factor availability, facilitates cell migration, and prevents persistent infections. These novel integrated therapies represent a breakthrough in addressing macroscopic wound healing while targeting the underlying microscale mechanisms. This invention also relates to methods for preparing, using, and administering the multifunctional composition for timely healing in mammals with various skin lesions.

A process for using bacterial or fungal metalloprotease and trypsin to solve problems associated with conventional protease extraction techniques by dramatically reducing temperature, incubation time and proteolysis during protease extraction. The present disclosure relates to a protease treatment for increasing yield from plant or other material by extracting nutrients from the fibrous waste portion of milled plant material while preserving the nutritional and functional qualities of the extracted material for use as a food product. The process preserves the quality of the extracted material, including beta glucan and protein, by utilizing low temperatures and minimal protease activity and digestion time during extraction.

The invention provides novel compositions and methods for the treatment of Radiation-Induced Bystander Effects (RIBE), resulting from radiation exposure. In one preferred embodiment the inventions includes novel therapeutic agents, including but not limited to quercetin and quercetin analogs, as well as E64, CA074, CA074Me, that interfere with the activity of Cathepsin B.

This disclosure provides several ribonuclease R variants and their preparation methods. Compared to the parent enzyme, the variants described in this disclosure exhibit increased protease activity per unit and show promising prospects for industrial applications. Furthermore, this disclosure also provides the application of ribonuclease R variants in linear RNA degradation.

The invention discloses a tannic acid-assisted antifouling coating for an inner seawater system as well as a preparation method and application of the tannic acid-assisted antifouling coating. According to the invention, tannic acid is used as an anchoring part of the antifouling coating to play a good fixing role on the surface of a base material through the adsorption effect, and then a physical interval is formed between tannic acid and the active center of protease through the connection of a hydrophilic substance in the middle layer, so that the conformation change or active site blockage possibly caused by tannic acid to protease is reduced, and the anti-fouling effect of tannic acid is improved. The activity of the protease is better maintained, and meanwhile, enough sites are provided for the protease, so that the protease can be effectively fixed on the surface of a base material to form an antifouling coating. Protein components in a biofilm matrix can be effectively degraded due to existence of protease on the surface of the antifouling coating, so that adhesion of bacteria and algae is prevented, and the antifouling coating has excellent antibacterial and anti-algae performance. The preparation method is simple, convenient, rapid, low in cost and suitable for preparation of the protective coating of the marine equipment and facilities in special scenes.

The invention discloses fermented beef jerky and a preparation method thereof. The preparation method comprises the following steps: (1) cleaning and cutting beef; (2) beef pretreatment; (3) performing staged fermentation on the pretreated beef; (4) carrying out gradient heating, drying and curing on the fermented beef; and preparing to obtain the finished beef jerky. Through strain compounding, the fermentation process is reasonably regulated and controlled, and the protease activity is controlled; water sudden loss is avoided through staged drying; finally, the hardness of the beef jerky is stabilized at 2,000-3,500 g, the chewiness is 1,200-2,000 g mm, and the beef jerky is free of powdery feeling, not sticky and suitable for consumers of all ages; meanwhile, the strains are compounded and synergistic, so that the a * value is more than or equal to 12, the decrease rate is less than or equal to 15% after storage for 3 months, the nitrite content is less than or equal to 3mg / kg, and the healthy consumption demand is met.

The invention discloses a preparation method and application of a hippophae rhamnoides fruit tea fermentation product, and the preparation method comprises the following steps: firstly preparing a hippophae rhamnoides fruit tea leachate according to a solid-liquid ratio of 1: 20-1: 100, then inoculating aspergillus cristatus according to an inoculum size of 1.5%-3.5%, fermenting for 3-12 days, and finally centrifuging and drying to obtain the hippophae rhamnoides fruit tea fermentation product. According to the method, the optimal material-liquid ratio, the inoculation amount and the fermentation time for preparing the hippophae rhamnoides fruit tea fermentation product are determined through the single factor level, the enzymatic activity of amylase, pectinase, protease and the like in the hippophae rhamnoides fruit tea fermentation product is remarkably improved, the protease activity is improved by 10 times or above, and digestion and absorption of macromolecular nutrient substances in the hippophae rhamnoides fruit tea are facilitated; the content of theabrownin in a sea-buckthorn tea fermented product is remarkably increased, the flavor and the physiological activity of the product are improved, a functional sea-buckthorn product rich in theabrownin is provided, and high-value utilization of sea-buckthorn resources is promoted; and harmful mycotoxins such as aflatoxin B1 are not detected, so that the safety is high.

This invention relates to a Bacillus velezensis strain and mutants thereof with surprisingly advantageous properties for a beneficial cleaning effect. These Bacillus strains are particularly good enzyme producers with high protease activity, in particular protease activity and cellulase activity, preferably protease, cellulase, and amylase activity. The protease activity is in particular secreted protease activity. The invention further relates to preparations of these Bacillus strains, a cleaning formulation (F) comprising the Bacillus strains according to the invention, a method for cleaning an object (O) or the human or animal body (B) as well as the use of the cleaning formulation (F) in cleaning an object (O) or the human or animal body (B). It further relates to a feed or food (E) comprising the Bacillus strains according to the invention and a method of feeding an animal.

We disclose a composition, comprising a compound, comprising a melanin precursor that spontaneously synthesizes a melanin when in free form in vivo, and a peptide directly covalently bonded to or indirectly linked to the melanin precursor, wherein the direct covalent bond or indirect link of the peptide to the melanin precursor blocks spontaneous synthesis of the melanin in vivo in the absence of protease activity. We also disclose methods of tumor imaging, tumor detection, diagnosis, and treatment, including methods comprising administering, to a patient suffering from a tumor, the composition; and either surgically resecting the tumor or thermally ablating the tumor, wherein the surgical resection is guided at least in part by contrast imparted by, or the thermally ablating targets cells containing, the melanin, wherein the melanin is spontaneously synthesized in the tumor after administering the composition.

The present application relates to anti-complement Cls antibodies and uses thereof. The present disclosure provides antibodies that bind complement Cls protein; and nucleic acid molecules encoding such antibodies. The present disclosure also provides compositions comprising such antibodies, and methods of making and using such antibodies, nucleic acid molecules, and compositions. The present disclosure provides an isolated, humanized monoclonal antibody that inhibits cleavage of complement component G4, wherein the antibody does not inhibit cleavage of complement component C2. In some cases, the antibody inhibits a component of the classical complement pathway, in some cases the classical complement pathway component is Cls. In some cases, the antibody does not inhibit protease activity of Cls.

The present application relates to a freshness indicator protein related to quality traits of low-temperature stored Siniperca chuatsi, endogenous proteases and applications. The freshness indicator endogenous proteases are related to postmortem proteolysis and meat softening of Siniperca chuatsi, including cathepsin B, cathepsin L and calpain. The freshness indicator proteins are related to quality changes occurring during postmortem cold storage of Siniperca chuatsi, including myoactin 1, myosin binding protein, LDB protein, actin-related protein, etc. The present application determines the change rule of endogenous protease activity of Siniperca chuatsi at different storage stages, and the dynamic influence on muscle proteolysis and muscle degradation of Siniperca chuatsi, and reveals the internal relationship with fish meat sensory quality and processing characteristics, and clearly defines the "critical point" of quality deterioration of Siniperca chuatsi. It provides clear guidance for the control of endogenous enzyme-mediated quality deterioration in the processing and storage process of Siniperca chuatsi.

The invention relates to the technical field of cosmetics, in particular to a blue copper peptide-containing composition and application thereof in cosmetics. The composition comprises the following components in parts by mass: 1 part of blue copper peptide; 0.5 to 5 parts of tetrahydromethylpyrimidine carboxylic acid; 0.05 to 0.5 part of sodium hyaluronate; and 0.05 to 0.5 part of beta-glucan. Preferably, the composition further comprises allantoin and lactobionic acid, based on 1 part by mass of the blue copper peptide, the content of the allantoin is 0.1-0.5 part, the content of the lactobionic acid is 0.2-1.0 part, and the mass ratio of the allantoin to the lactobionic acid is 1: (2-4). In-vitro efficacy experiments prove that the specific combination and proportion can generate a remarkable synergistic effect, and meanwhile, the capabilities of inhibiting the activity of elastinase and promoting epidermal cell repair are improved, so that the excellent dual effects of wrinkle resistance and skin barrier repair are realized. The composition can be used for preparing cosmetics such as essence, emulsion and face cream.

The application belongs to the technical field of biosensing, and particularly relates to a giant magnetoresistance biosensor for detecting protease, a preparation method and application thereof, which comprises a giant magnetoresistance sensor, a glass sheet plated with a gold film, a functional polypeptide and metal ion chelated magnetic beads; wherein the glass sheet plated with the gold film is fixed on the giant magnetoresistance sensor, one end of a peptide chain of the functional polypeptide is connected with the glass sheet plated with the gold film, and the other end is connected with the metal ion chelated magnetic beads; the giant magnetoresistance biosensor provided by the application can realize monitoring of protease activity, has high sensitivity, strong anti-interference ability, stable performance, and is easy to integrate, so that the giant magnetoresistance biosensor can develop into a portable detection device for large-scale promotion, and has wide application prospects in the fields of diagnosis, treatment and related drug development of major diseases.

The invention belongs to the technical field of cosmetics, and particularly relates to a Keemun black tea-root composition with repairing and anti-aging effects as well as a preparation method and application of the Keemun black tea-root composition. The Keemun black tea root extract, the elm root extract and the acanthopanax root extract in a specific amount ratio range are scientifically compounded, the synergistic effect among the components is remarkable, the effect limitation of a single component is effectively overcome, and the synergistic enhancement of anti-aging and repairing effects is realized. Experiments and exploration find that the composition can significantly inhibit the activity of elastase and delay the skin aging process caused by elastin degradation; meanwhile, the transdermal moisture loss can be obviously improved, and the skin barrier function and the self-repairing capability are enhanced. Therefore, the Keemun black tea-root composition disclosed by the invention is outstanding in overall effect, has the characteristics of naturalness, high efficiency and mildness, and accords with the development trend of modern skin care products.

The application belongs to the field of food preservation technology, and relates to a chitosan-gelatin composite preservative solution based on plasma-activated water modification and a preparation method and application thereof. First, PAW is used to prepare chitosan solution and gelatin solution respectively, and the composite preservative solution is obtained after mixing and homogenizing. The coating is dehydrated to form a preservative film. The PAW is prepared by treating deionized water with low-temperature plasma, and is rich in active ingredients such as hydroxyl radicals and hydrogen peroxide, which can effectively modify chitosan and gelatin, enhance intermolecular crosslinking, and form a composite film with a dense network structure. The composite film can be applied to fish preservation through coating or immersion, can effectively inhibit the growth of microorganisms, and can improve the color of fish fillets. In addition, the composite film has the function of delaying protein degradation by inhibiting endogenous protease activity, thereby maintaining the muscle tissue structure and prolonging the shelf life of chilled fish fillets. The application has the advantages of simple process, green safety, remarkable preservation effect, and wide market application prospect.

The invention relates to application of an MI-2 compound in preparation of a medicine for treating acute myelogenous leukemia, and belongs to the technical field of biological medicine. It is proved for the first time that in AML cells without MALT1 protease activity, the compound MI-2 (MALT1 inhibitor) can inhibit cell proliferation and induce apoptosis through a brand new mechanism independent of MALT1 inhibition, namely specific regulation and control of lipid metabolism of the AML cells, and therefore the compound MI-2 (MALT1 inhibitor) serves as a candidate drug or a brand new treatment strategy for treating acute myelogenous leukemia for the first time.