228 results about "Essential amino acid" patented technology

Soybean plants producing seeds and soybean seeds comprising increased protein content are provided. Also provided are soybean plants producing seeds and soybean seeds comprising increased amounts of essential amino acids such as methionine, tryptophan or lysine. Protein compositions having increased essential amino acids can be made from the soybean seeds. Additionally, methods for generating and using plants, seeds and protein compositions having increased protein content or increased amounts of essential amino acids are disclosed.

The invention relates to the field of cell biology, and particularly discloses a method for preparing mesoderm progenitor cells from human pluripotent stem cells and an induced differentiation culture medium of the mesoderm progenitor cells. The method comprises the following steps: inoculating human pluripotent stem cells in a single cell state into a culture dish covered with matrigel; and inducing the human pluripotent stem cells to be differentiated into the mesoderm progenitor cells within 1-3 days by using the mesoderm progenitor cell induced differentiation culture medium. Wherein the mesoderm progenitor cell induced differentiation culture medium is composed of a basic culture medium and an additive, and the additive contains a B-27 additive, non-essential amino acid, glutamine, ascorbic acid, polyvinyl alcohol, a GSK-3 inhibitor and a CDK8 inhibitor. According to the method, the human pluripotent stem cells are subjected to induced differentiation by using the mesoderm progenitor cell induced differentiation culture medium with specific composition, the method does not depend on cloning of the pluripotent stem cells, a large number of high-uniformity mesoderm progenitor cells can be obtained through induced differentiation within 1-3 days, the induction efficiency is high, and the stability is high.

The present invention discloses a complete medium for culturing DF-1 cells, including DMEM basal medium 13.7g / L, insulin, non-essential amino acids, transferrin, glutathione, ascorbyl phosphate, sodium selenite, ethanolamine, hydrocortisone, and newborn calf serum. Wherein non-essential amino acids include glycine, alanine, proline, serine, and asparagine. A complete medium for culturing DF-1 cells provided by the present invention can well promote the proliferation of DF-1 cells, can achieve the same proliferation effect as high-concentration fetal bovine serum, and is suitable for the adherent culture of DF-1 cells; using low-concentration newborn calf serum, it is possible to greatly avoid the batch differences, unstable culture results, cytotoxicity, and high risk of carrying pathogens brought by the culture medium with high-concentration serum, narrow application range, expensive fetal bovine serum, and high culture cost; the components are simple, the culture medium preparation process is short in time consumption, the culture efficiency is high, the repeatability is good, and it is suitable for batch production.

The invention provides a pichia pastoris strain for efficiently converting methanol to produce high-methionine-content single-cell protein and application of the pichia pastoris strain, and belongs to the technical field of biology. According to the invention, a strain of pichia pastoris (named as Pichiapastoris CN-SCP 00 with a preservation number of CCTCC (China Center For Type Culture Collection) NO: M2025657) is obtained through separation, and is subjected to multiple rounds of ARTP iterative mutation breeding, so that a strain Pichiapastoris CN-SCPYE421 (with a preservation number of CCTCC NO: M2025658) for efficiently converting methanol to produce single-cell protein containing high methionine is obtained. Compared with a commercial strain, the Pichiapastoris CN-SCPYE421 has higher methanol tolerance and utilization capacity, the fermentation time is remarkably shortened, and in addition, the Pichiapastoris CN-SCPYE421 can well grow at the temperature of 35 DEG C. The Pichiapastoris CN-SCPYE421 is subjected to fermentation culture in a 5L fermentation tank, the dry weight of thalli reaches 120-150g / L, the protein content reaches 65-75% of the dry weight of the thalli, the Pichiapastoris CN-SCPYE421 is rich in various essential amino acids, and the methionine content reaches 2.3%. The invention provides an excellent strain for industrial development of single-cell protein, and has great social significance and economic value for the feed protein industry.

The invention discloses a culture medium and a method for performing in-vitro 3D cell model construction on skin tissues. The invention provides a culture medium for constructing a skin tissue in-vitro 3D (three-dimensional) cell model. The culture medium is prepared from an antibacterial and antifungal agent tri-antibody, HEPES, GlutaMax, MEM non-essential amino acid, human recombinant protein EGF, bFGF, HGF, FGF-10, Wnt-3a, Noggin, R-spondin 1, IL-2, IL-15, IGF-I, CHIR99021, SB202190, A83-01, Primocin, N-acetyl-L-cysteine, nicotine, an N2 additive, B27, ITS-X, Y-27632, dexamethasone, calomel and a basal culture medium. The skin tissue in-vitro 3D cell model constructed by the invention has a wide application prospect in the fields of basic scientific research and clinical diagnosis and treatment of skin diseases.

The invention relates to a Kluyveromyces marxianus strain with high protein yield and application of the Kluyveromyces marxianus strain, which is characterized in that the Kluyveromyces marxianus strain is firstly cultured on a slant, then is transferred into a seed culture medium for seed preparation, and then is inoculated into a fermentation tank, and yeast protein is obtained by adopting index feeding; the bacterial strain is a dominant mutant strain Kluyveromyces marxianus YWX-1, the protein content in a dry thallus reaches 55% or above and is increased by 26% or above compared with that of an original bacterial strain, the bacterial strain can be used for production of single-cell protein, the essential amino acid index (EAAI) value in the protein reaches 1.2, and the bacterial strain is rich in arginine, threonine and lysine and can be applied to production of single-cell protein. These amino acids play a key role in immune regulation, intestinal mucosal barrier function and muscle protein synthesis of young animals. The YWX-1 kluyveromyces marxianus strain has a wide application prospect in the production of neoplasm protein.

The invention discloses kluyveromyces marxianus and application of the kluyveromyces marxianus in production of single-cell protein by taking straw as a raw material. The Kluyveromyces marxianus is a ZH-2 bacterial strain which is CGMCC (China General Microbiological Culture Collection Center) No.32295 The strain is obtained from a wild strain in a laboratory adaptive evolution mode, wheat straw and an inorganic nitrogen source can be used as raw materials for efficiently producing single-cell protein, compared with a parent strain, the utilization speed and the utilization rate of biomass, glucose and xylose are remarkably increased, the yield of ZH-2 SCP can reach 0.047 g.g <-1 >, and the yield of ZH-2 SCP can reach 0.045 g.g <-1 >. And the essential amino acid mode basically accords with adult and infant amino acid modes, and can be used as high-quality protein nutrition supply. The Kluyveromyces marxianus ZH-2 strain capable of obtaining single-cell protein by fermenting wheat straw as a raw material is provided from the perspective of utilizing cheap waste resources, so that the production cost of SCP is greatly reduced, and the problem of protein resource shortage is effectively solved.

The invention provides a methylobacterium strain JC03 for producing single-cell protein and application of the methylobacterium strain JC03 in the field of aquaculture, and belongs to the technical field of microorganisms. The methylobacterium strain JC03 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.32616. The strain is high in growth efficiency, high in thallus crude protein content, rich in amino acid spectrum, high in essential amino acid content and rich in functional components for improving the immunocompetence of aquatic animals; the single-cell protein produced by the strain can completely or partially replace fish meal protein in feeds of marine fishes such as turbot, pseudosciaena crocea, grouper and rainbow trout; vegetable protein in feed of freshwater fish such as grass carp can be completely replaced; animal and plant proteins in feeds for shrimps and crabs, such as penaeus vannamei, can be completely replaced, and animal and plant proteins in stichopus japonicus feeds can be completely replaced; the immunocompetence of fed aquatic animals can be obviously improved. The invention provides a microbial strain for efficiently converting methanol to produce single-cell protein, and the microbial strain has a wide application prospect in the field of aquaculture.

The present invention refers to pharmaceutical compositions comprising ibuprofen or a pharmaceutically acceptable derivative thereof and one or more solubilizing agents in the form of A) aqueous solution for use in intravenous administration comprising ibuprofen of either the sodium or lysinate salt, paracetamol and preferably one or more cyclodextrins or B) aqueous solution for use in intravenous administration wherein the one or more solubilizing agents are one or more essential amino acids and optionally one or more cyclodextrins or C) composition for use in oral administration, which comprises paracetamol and cyclodextrin, wherein said composition is in the form of either i) effervescent tablets or effervescent granules or ii) oral suspension or syrup or oral solution or iii) soft capsules.

The invention provides a method for cultivating volvariella volvacea by taking bamboo reed as a substrate raw material. Evaluation from multiple dimensions such as the growth speed, commodity characters and nutritional ingredients of the straw mushrooms finds that the normal growth of the straw mushrooms is not influenced by adding a proper amount of the bamboo reed, and the content of the nutritional ingredients such as protein, essential amino acid and mineral substances can be increased. However, when the content of the bamboo reed in the culture medium is too high, the growth condition and commodity characters of the volvariella volvacea can be influenced to a certain extent, and the best alternative culture medium is obtained by matching 60% of the bamboo reed with 40% of the cottonseed hulls according to the comprehensive results.

Disclosed herein are formulations and methods of administering formulations to starve cells of nutrients, such as amino acids. The formulations can be substantially devoid of one or more non-essential amino acids. A formulation disclosed herein can be given to a subject to treat a disease, for example, cancer. Removing a particular amino acid or a combination of amino acids from the diet of a subject can lead to improved treatment of various diseases, including cancer. Reduction of amino acid levels in a subject reduces the production of proteins, metabolites, lipids, or nucleic acid that can promote cancer growth and metastasis.

The invention relates to the technical field of biological medicine, in particular to I-type humanized collagen as well as a preparation method and application thereof. The amino acid sequence of the type I humanized collagen prepared by the invention is shown as a sequence SEQ ID NO. 1 (HTGAWGKTVIEYKTTKTSRLVMGFXGPKGAA, wherein X is hydroxyproline). The invention also discloses a preparation method of the type I humanized collagen. The I-type humanized collagen contains essential amino acids and semi-essential amino acids of a human body, promotes proliferation of human skin fibroblasts, up-regulates expression of elastin, fibronectin and collagen I genes, and has the effects of interacting with endothelial cells and osteoblasts, promoting creeping and growth of the osteoblasts, and promoting the growth of the osteoblasts. And the interaction between osteoblasts and collagen is promoted. Therefore, the I-type humanized collagen can be widely applied to orthopedic sports materials and has application prospects in the fields of medical cosmetology and sports medicine materials.

The invention discloses a preparation method of a nano-liposome coated NAD amino acid nutrient solution, and relates to the technical field of liposome amino acid nutrient solution preparation, the preparation method comprises the following steps: S1, preparing raw materials: S1.1, preparing NAD amino acid; s1.2, lecithin: using pure natural lecithin from soybeans and eggs; s1.3, preparing sodium hyaluronate: adopting sodium hyaluronate with low molecular weight; s1.4, preparing an amino acid compound, wherein the amino acid compound contains a plurality of essential amino acids, including glycine, serine and lysine; according to the mask, when the NAD amino acid nutrient solution wrapped by the nano-liposome is applied to the mask, the mask can more effectively permeate into the deep layer of the skin due to the excellent stability and biological activity of the NAD amino acid nutrient solution, and sufficient nutrition and moisture are provided for the skin; meanwhile, due to the strong anti-aging effect, the mask can remarkably improve the skin texture, reduce fine wrinkles and wrinkles, and make the skin more compact, smoother and elastic.

The invention relates to aspartate kinase for relieving feedback inhibition of L-threonine and application of aspartate kinase, and belongs to the field of enzyme engineering and metabolic engineering. The mutant is obtained by carrying out E253K and / or K507E mutation on the basis of wild type aspartate kinase as shown in SEQ ID NO.1, the enzyme activity of the mutant is not obviously changed under the condition that the concentration of L-threonine is 0-12 mmol / L, and the feedback inhibition effect of L-threonine on the mutant is relieved. The method can be widely applied to synthesis of essential amino acids including L-threonine, L-tryptophan, L-isoleucine, L-lysine, L-leucine, L-valine, L-methionine, L-phenylalanine and the like.

The invention provides an establishment method and an evaluation method of a model for monitoring and calculating the maintenance demand amount of essential amino acids, and the establishment method of the model for monitoring and calculating the maintenance demand amount of the essential amino acids comprises the following steps: the method is applied to monitoring and calculating the dynamic maintenance demand amount of broiler chicken essential amino acids; an automatic weighing system is used for recording the growth performance of the weight, weight gain and food consumption of the broiler chickens every day; collecting excrement of the broiler chickens by adopting a full excrement collecting method for continuous 3 days per week in the growth period of the broiler chickens, recording feed intake and excrement discharge amount, and calculating the remaining amount of each necessary amino acid per week; and performing multiple linear regression equation fitting according to the obtained growth performance and the amino acid remaining amount, and setting the weight gain to be 0 on the basis of the equation to obtain a new regression equation, namely the dynamic essential amino acid maintenance demand model. The structure and components of daily ration for feeding animals do not need to be changed, so that the stress generated when the animals are in contact with different daily ration can be eliminated, the real necessary amino acid maintenance required amount of the animals can be reflected, and the purpose of precise nutrition is achieved.

The invention provides a preparation method of a low-salt high-quality fermented seasoning by adopting nano dissolution catalysis and ultraviolet sterilization treatment. The preparation method is mainly characterized in that raw materials are subjected to ultra-fine pulverization treatment and non-thermal sterilization treatment, and bioactive substances derived from various plant original taste components are added. Specifically, won is subjected to superfine pulverization to the size of 20-400 nm, and non-thermal sterilization methods such as high-pressure treatment, pulsed electric field or low-temperature plasma and the like are repeated for 2-7 times. In addition, the addition amount of bioactive substances (polyphenols, dietary fibers, essential amino acids and the like) extracted from 15-35 vegetable litchis selected according to a specific standard is in a range of 0.2-4%. The salinity is reduced to 30-70% of that of an existing product, meanwhile, the flavor and freshness are kept, and the low-sodium fermented seasoning with the health-care functions of reducing sodium absorption, inhibiting blood pressure rise, promoting growth of beneficial bacteria in intestinal tracts and the like can be produced. The invention provides an innovative manufacturing method, which not only can meet the requirements of consumers on health, but also can provide high-quality fermented seasoning.

The invention discloses a formula and a preparation method of meal replacement porridge for children. The meal replacement porridge comprises the following raw materials: fried brown rice, millet, whey protein, buckwheat, black beans, carrots, shiitake mushrooms, haws, Chinese dates and walnut kernels. Various coarse grains are used as main raw materials, so that the food is high in fiber content, rich in protein, fat and carbohydrate, capable of achieving the effect of supplementing nutrition for children, capable of enabling an eater to have satiety, capable of promoting intestinal peristalsis and capable of improving the appetite of the eater. Lactalbumin, carrots, shiitake mushrooms, haws, jujubes and walnut kernels are added into the raw materials to increase essential amino acids, various vitamins and minerals for children and enhance the autoimmunity of children, and the jujubes and walnut kernels have the effects of tonifying kidney and dredging brain, promote heat consumption and metabolism in children bodies, and are beneficial to weight losing and weight control; and daily nutritional requirements of children can be met.

Nutraceutical food supplement for treatment of young women in post-puberty age suffering from polycystic ovary syndrome with symptoms like irregular menstruation cycles, insulin resistant diabetes, infertility, hair loss, acne and weight gain is provided. Formulations having myo-inositol and its D-chiro isomer along with plant proteins, a carbohydrate (inulin) and other plant fibres; along with well-defined proportions of vitamins (A, B1 to 6; B12 and C); several minerals like Ca, Cr, Fe, K, Mg, Mn, Na and Zn and essential amino acids like L-Glutamine, L-Lysine, and L-Taurine are provided.

The invention provides an in-vitro embryo culture solution and a preparation method thereof.The IVM solution of the in-vitro embryo culture solution comprises CaCl2, KCl, KH2PO4, glycine, HEPES sodium salt, lactic acid, MgSO4, NaHCO3, inositol, glucose, essential amino acid, non-essential amino acid, retinoic acid, taurine and hormones, and when the in-vitro embryo culture solution is used as mammals such as cows, the in-vitro embryo culture solution can be used for preparing the in-vitro embryo culture solution. And the IVF embryo production efficiency and embryo quality of the cattle can be improved.

The invention discloses a method for improving the quality of ganoderma lucidum mushroom residues through synergistic fermentation of bacteria and enzymes and application of the method. According to the method disclosed by the invention, cellulase, beta-galactosidase and xylanase with proper concentrations and lactobacillus plantarum, bacillus subtilis and saccharomyces cerevisiae with proper proportions are adopted, and enzymes and bacteria are mutually cooperated, so that the characteristics of the raw materials are well balanced, the finally prepared ganoderma lucidum mushroom bran is high in protein content and low in fiber content, and the nutritional value of a feed is improved; the total amount of amino acids and essential amino acids are remarkably increased, and the feed protein quality can be improved; the rumen dry matter digestibility and energy metabolites are remarkably improved, and ruminant absorption and utilization are facilitated; the ganoderma lucidum mushroom bran fermented feed is added into the daily ration, so that the daily gain, the carcass rate and the dressing percentage of fed animals can be remarkably improved, the levels of serum GLU, TG, ALT and AST are reduced, the expression of key inflammatory cytokines (IL-1beta and IL-6) is inhibited, and the ganoderma lucidum mushroom bran fermented feed has the comprehensive effects of regulating glycolipid metabolism and relieving liver load and systemic inflammation, so that the immune function of animal bodies is improved.

Compositions for controlling ants include one or more essential amino acids (EAAs); one or more sugar compounds; and a lethal agent. Such compositions can be liquid bait compositions. A method of using such compositions can include deploying compositions in an ant bait station and positioning the ant bait station near an ant nest.

The invention belongs to the technical field of medicines, and particularly relates to a dipeptide capable of preventing and delaying colorectal cancer and application of the dipeptide. The invention provides a dipeptide for preventing and delaying colorectal cancer, which has the effects of inhibiting the activity and proliferation of colorectal cancer cells and can delay the development of colorectal cancer. And the product can be easily absorbed by human bodies, has the capability of supplementing essential amino acids for human bodies and a nutritional function, and has a good application prospect as a medicine for preventing and delaying development of colorectal cancer.

The invention provides a feed traditional Chinese medicine additive for improving chicken quality of broiler chickens and application thereof, and relates to the field of animal feeds, and the feed traditional Chinese medicine additive provided by the invention comprises the following raw material components in parts by mass: 10-30 parts of radix tetrastigme stems and leaves, 1-2 parts of pomegranate rind, 0.5-1 part of dried orange peel and 0.5-1 part of liquorice. By using the feed traditional Chinese medicine additive and the broiler feed provided by the invention, the contents of flavor amino acids such as glutamic acid, alanine and threonine and essential amino acids such as phenylalanine, lysine and isoleucine can be increased, the amino acid composition of broilers is improved, the flavor of broiler chicken is improved, and the quality of broiler chicken is improved; the abundance of beneficial bacteria such as Prevoteraceae UCG001 and the like can be improved, the abundance of harmful bacteria such as Mucispirillum and the like can be reduced, and meanwhile, the intestinal nutrition transfer can be enhanced and the inflammatory response in vivo can be relieved.

The present invention provides a culture medium and a culture method for primary human acute myeloid leukemia cells. The primary acute myeloid leukemia cell culture medium of the present invention comprises a glutamine additive, non-essential amino acids, human interleukin-6, human interleukin-7, human interleukin-3, recombinant human FLT3Ligand, recombinant human macrophage colony-stimulating factor, and human stem cell factor. Using the culture medium and culture method of the present invention, acute myeloid leukemia cells can be cultured with higher amplification efficiency and longer in vitro culture time. The present invention also provides a method and application for human primary acute myeloid leukemia cells cultured in vitro using the culture medium for use in drug efficacy evaluation and screening.

The invention relates to the technical field of water-soluble fertilizers, and particularly discloses a method for preparing a water-soluble fertilizer by using a subcritical hydrolysis technology, which comprises the following steps: selecting ingredients, selecting animal raw material feather, steaming and crushing to obtain feather powder, selecting plant raw material soybean meal, crushing and deoiling to obtain soybean meal powder, selecting microbial raw material mushroom bran, drying and crushing to obtain the water-soluble fertilizer. Selecting deionized water, sulfuric acid and urea; the feather meal is rich in high-content keratin, the feather meal can be decomposed into amino acid and short-chain peptide in the subcritical hydrolysis process by adding the feather meal, a quick-acting nitrogen source capable of being directly absorbed by plants is provided, meanwhile, the sulfur element is released, and the defect of sulfur in a traditional fertilizer is overcome; the small molecule peptide and the free amino acid are complementary with the amino acid spectrum of the feather meal, so that a more comprehensive plant nitrogen source is formed.

Provided is a reduced β-nicotinamide mononucleotide salt-type compound as represented by formula (I), and particularly provided are a reduced β-nicotinamide mononucleotide salt and a composition thereof, a preparation method therefor, and a use thereof. The salt-type compound may serve as an active ingredient in a pharmaceutical composition for enhancing NAD+ to delay aging and / or for preventing or treating diseases or symptoms induced or caused by NAD+ deficiency and for preventing and / or treating diseases or symptoms induced or caused by deficiencies in essential elements and / or essential amino acids of the human body. In addition, compared with NMNH disodium salt crystalline and amorphous forms, the reduced β-nicotinamide mononucleotide salt-type compound exhibits long-term stability and moisture absorption resistance and is more beneficial to long-term storage and market promotion.

The invention relates to a genetically engineered bacterium for efficiently synthesizing ectoine and a construction method of the genetically engineered bacterium. The genetically engineered bacterium comprises a related protein degradation tag, an expression vector and a construction method of the expression vector. According to the invention, a protein degradation tag is added to regulate and control a diaminopimelate decarboxylase gene lysA and a homoserine dehydrogenase gene metL, and meanwhile, an exogenous gene cluster ectABC is introduced to construct an expression path of ectoine in a host cell so as to realize de novo synthesis of ectoine. By means of the construction method, the content of essential amino acid needed by life metabolism of thalli is guaranteed, growth of the strains is promoted and affected, and the purpose of high yield of ectoine is achieved. The amino acid sequence of the protein degradation tag is mutated on the basis of regulation and control of the protein degradation tag, and it is found that the mutated protein degradation tag is more beneficial for increasing the yield of ectoine.

The present application belongs to the technical field of feed additive, and discloses a kind of feed additive for improving the quality of Nibea albipunctata swim bladder, which is composed of the following components by mass fraction: 40-55wt% cottonseed protein, 25-38wt% soybean saponin, 10-16wt% capric acid monoglyceride, 8-10wt% alanyl glutamine. In the above-mentioned feed additive, alanyl glutamine and capric acid glyceride play an important role in the process of synthesizing swim bladder collagen protein and accumulating essential amino acids in collagen protein. The mutual cooperation between the components in the feed additive can promote the deposition of swim bladder collagen protein and improve the accumulation of essential amino acids in collagen protein at the same time.

The present application relates to formulations for individualized methods of treatment. Disclosed herein are formulations and methods of administering formulations to lack cells with nutrients, such as amino acids. The formulations of the present invention may be substantially free of one or more non-essential amino acids. Disclosed herein is a composition comprising in unit dosage forms: a) a non-essential amino acid or a salt thereof wherein the non-essential amino acid or the salt thereof is not part of a polypeptide; b) a first essential amino acid or a first salt thereof and a second essential amino acid or a second salt thereof wherein the first essential amino acid or the first salt thereof and the second essential amino acid or the second salt thereof are present in the composition in an equal amount, wherein the first essential amino acid and the first salt thereof and the second essential amino acid and the second salt thereof are not part of a polypeptide; and c) a pharmaceutically acceptable excipient.

The invention discloses a culture medium for in-vitro adherent culture of primary tumor cells and application, and relates to the technical field of tumor cell culture. Comprising a basic culture medium, fetal calf serum FBS with the concentration of 5 ng / mL-15 ng / mL, an epidermal growth factor EGF with the concentration of 1 ng / mL-10 ng / mL, a basic fibroblast growth factor bFGF with the concentration of 0.5 ng / mL-5 ng / mL, an insulin-transferrin-selenium ITS supplement with the final volume concentration of 0.1%-1%, L-glutamine with the final volume concentration of 0.5 mM-2 mM, ascorbic acid with the final volume concentration of 0.1%-0.5% and non-essential amino acid with the final volume concentration of 1%-5%. The kit comprises 10 [mu] g / mL-50 [mu] g / mL of heparin and 1 [mu] g / mL-5 [mu] g / mL of an Rho kinase inhibitor. The culture medium overcomes the defects that in the prior art, when primary tumor cells are subjected to adherent culture, apoptosis or dedifferentiation is prone to occurring, in-vivo tumor characteristics are difficult to simulate, the tumor growth speed is low, the proliferation effect is poor, and the adherent rate is low.