908 results about "Essential amino acid" patented technology

Methods and materials for culturing primate-derived primordial stem cells are described. In one embodiment, a cell culture medium for growing primate-derived primordial stem cells in a substantially undifferentiated state is provided which includes a low osmotic pressure, low endotoxin basic medium that is effective to support the growth of primate-derived primordial stem cells. The basic medium is combined with a nutrient serum effective to support the growth of primate-derived primordial stem cells and a substrate selected from the group consisting of feeder cells and an extracellular matrix component derived from feeder cells. The medium further includes non-essential amino acids, an anti-oxidant, and a first growth factor selected from the group consisting of nucleosides and a pyruvate salt.

The growth hormone supergene family comprises greater than 20 structurally related cytokines and growth factors. A general method is provided for creating site-specific, biologically active conjugates of these proteins. The method involves adding cysteine residues to non-essential regions of the proteins or substituting cysteine residues for non-essential amino acids in the proteins using site-directed mutagenesis and then covalently coupling a cysteine-reactive polymer or other type of cysteine-reactive moiety to the proteins via the added cysteine residue. Disclosed herein are preferred sites for adding cysteine residues or introducing cysteine substitutions into the proteins, and the proteins and protein derivatives produced thereby.

The growth hormone supergene family comprises greater than 20 structurally related cytokines and growth factors. A general method is provided for creating site-specific, biologically active conjugates of these proteins. The method involves adding cysteine residues to non-essential regions of the proteins or substituting cysteine residues for non-essential amino acids in the proteins using site-directed mutagenesis and then covalently coupling a cysteine-reactive polymer or other type of cysteine-reactive moiety to the proteins via the added cysteine residue. Disclosed herein are preferred sites for adding cysteine residues or introducing cysteine substitutions into the proteins, and the proteins and protein derivatives produced thereby.

A defined serumfree medical solution for applications in Ophthalmology, that contains one or more cell nutrient supplements, and a growth factor(s) which maintains and enhances the preservation of eye tissues, including human corneal, retinal and corneal epithelial tissues at low to physiological temperatures (2 DEG C. to 38 DEG C.). This solution is composed of a defined aqueous nutrient and electrolyte solution, supplemented with a glycosaminoglycan(s), a deturgescent agent(s), an energy source(s), a buffer system(s), an antioxidant(s), membrane stabilizing agents, an antibiotic(s) and / or antimycotic agent(s), ATP or energy precursors, nutrient cell supplements, coenzymes and enzyme supplements, nucleotide precursors, hormonal supplements, non-essential amino acids, trace minerals, trace elements and a growth factor(s).

Compostions of GLA and / or other EFAs with TA or related compounds, and their use in therapy or nutrition or in preparation of composition for therapy or nutrition, especially to improve cell membrane EFA concentration and / or (particularly in diabetic complications) impaired nerve function and blood flow.

A nutritional formula for PKU patients. The nutritional formula has, as a protein source, a mixture of caseino-glyco-macropeptide and complementary essential amino acids other than Phe to provide a balanced amino acid profile. Vitamins and minerals sufficient to meet daily requirements are also included. When used as a complete diet, the nutritional formula includes a carbohydrate source and a fat source. However the nutrutional formula may also be used as a protein supplement in which case the carbohydrate source and the fat source may be omitted. The nutritional formula has a pleasant taste.

The invention provides a cell cryo-preserved liquid, an application, and an immune cell cryo-preservation method. The cell cryo-preserved liquid includes 0.7-0.9 ml / ml of a cell culture medium, 8-26 mg / ml of non-essential amino acids, 0.04-0.1 ml / ml of trehalose, 0.5-10 mg / ml of vitamin C, 10-50 mg / ml of human albumin, 0.04-0.12 ml / ml of propylene glycol, 0.01-0.07 ml / ml of dimethyl sulfoxide and 0.5-3 mg / ml of lentinan. Compared with a cell cryo-preserved liquid in the prior art, the human albumin can replace fetal calf serum to achieve the effects of the fetal calf serum in the cell cryo-preserved liquid, and meanwhile, the cell cryo-preserved liquid is added with the non-essential amino acids, the vitamin C and the lentinan, thereby ensuring cell proliferation vitality after recovery is not influenced. The trehalose and the propylene glycol can replace a protective effect of dimethyl sulfoxide, thereby ensuring moisture in cells to be not crystallized during approach of freezing point. The components are cooperated with each other to achieve cryo-preservation of immune cells.

The present invention relates to the compositions that enhance post-exercise recovery processes to increase both strength and muscle mass, replace glycogen stores, and prevent inflammation, resulting in the prevention and / or reduction of delayed onset muscle soreness. Additionally, it provides a feeling of muscle relaxation as well as a feeling of mental tranquility immediately following exercise. The composition consists of any or all high-glycemic sugars and / or polysaccharides (e.g., sucrose, glucose, maltodextrin), all essential amino acids and beta-hydroxy-beta-methylbutyrate and can include other amino acids sources (e.g. whey protein), performance enhancing agents (e.g., caffeine, L-glutamate), anti-inflammatory agents (e.g., ginger, boswellia, curcumen), antioxidants (vitamin C, vitamin E, selenium, polyphenols,), insulin-mimicking agents (cinnamon, Banaba), analgesics (e.g. aspirin, ibuprofen, naproxen, acetaminophen), and to methods of treating humans and animals by administration of these novel compositions to humans and animals in need thereof.

The invention discloses a meat scent savoury and pungent flavoring, containing the following raw materials: vegetable oil, dried pimiento, zanthoxylum bungeanum, star anise, salt, sugar, monosodium glutamate, starch, meat reaction solution, fresh increasing flavoring agent. A production method of the meat scent savoury and pungent flavoring includes: heating the vegetable oil to 150-200 DEG C; respectively adding the dried pimiento zanthoxylum bungeanum and anise tree and frying for 30-60 seconds, quickly removing, draining the oil, and then coarse crushing and mixing the dried pimiento zanthoxylum bungeanum and anise tree; adding salt, sugar, monosodium glutamate, starch, meat reaction solution in the mixture, mixing uniformly; and then adding the fresh increasing flavoring agent, and then mixing uniformly; performing ultramicro crushing after microwave drying, and then obtained the completed product by hermetic package. The invention uses a short time fried mode to alleviate the biting taste of the pimiento and prominent the fragrant flavor of the pimiento; and matches with flavoring made up by different meat reaction solutions, so that the invention contains not only the meat flavor, but also an increase of essential amino acids of human body, minerals and so on.

The invention discloses a serum-free medium for culturing placenta mesenchymal stem cells. The serum-free medium takes a DMEM (Dulbecco Modified Eagle Medium) culture solution as a basis and also contains a fibroblast growth factor receptor 2, growth hormone, insulin, transferrin, glutathione, BMP-4, L-glutamine, sodium pyruvate, non-essential amino acids and beta-mercaptoethanol. According to various serum-free media provided by the invention, growth and proliferation of the placenta mesenchymal stem cells in a serum-free medium system can be effectively promoted, the placenta mesenchymal stem cells have higher growth and proliferation rate in the serum-free medium system compared with a serum cell culture medium, the characteristics of the stem cells are preserved, the serum-free medium has multiple differential potentials, and the stem cells can be directionally induced into fat cells and osteoblasts.

An amino acid composition with high nutrition supporting effect is composed of acetylcysteine, acetyltyrosine and tryptophan in weight ratio of (0.81-2): (0.81-2): (0.9-1.6).

The growth hormone supergene family comprises greater than 20 structurally related cytokines and growth factors. A general method is provided for creating site-specific, biologically active conjugates of these proteins. The method involves adding cysteine residues to non-essential regions of the proteins or substituting cysteine residues for non-essential amino acids in the proteins using site-directed mutagenesis and then covalently coupling a cysteine-reactive polymer or other type of cysteine-reactive moiety to the proteins via the added cysteine residue. Disclosed herein are preferred sites for adding cysteine residues or introducing cysteine substitutions into the proteins, and the proteins and protein derivatives produced thereby. Also disclosed are therapeutic methods for using the cysteine variants of the invention.

The invention relates to an application of a Zea mays grain transcription factor in the aspect of regulation and control of alcohal-soluble proteins. The factor comprises a base sequence shown in SEQ ID NO: 1. The protein ZmbZIP22 encoded by the sequence can be directly bonded to a 27kDa gamma-gliadin promoter and activate the 27kDa gamma-gliadin promoter. Gene-deficient mutant plants are obtained through carrying out Zea mays immature-embryo conversion by taking a gene fragment, shown in SEQ ID NO: 2, of ZmbZIP22 as a guide RNA by using a CRISPR-Cas9 technology. Compared with wild type grains, transgenic mutants have Zea mays grains with irregular and relative-thin protein body shells, the content of alcohal-soluble proteins in mature grains is remarkably lowered, the content of essential amino acids such as lysine, which are deficient to the conventional Zea mays, in the mature grains is remarkably increased, and thus, the nutritional quality of Zea mays is improved.

The present disclosure provides formulations and methods for delivering water-soluble and lipid-soluble nutrients for preventing or correcting nutrient deficiencies to subjects requiring small-volume nutritional support, such as preterm infants. The formulations may comprise an emulsion of docosahexaenoic acid (DHA) stabilized by a protein emulsifier, such as α-lactalbumin, and may further comprise other valuable nutrients, such as arachidonic acid (ARA), arginine, glutamine, arginyl-glutamine dipeptide and / or alanyl-glutamine dipeptide. The formulation is useful, for example, for correcting nutritional deficiencies by increasing a subject's intake of nutrients such as ω-3 or ω-6 long-chain polyunsaturated acids, proteins, peptides, vitamins, minerals, other fatty acids, and / or essential amino acids. The nutritional formulation is suitable for enteral delivery and small-volume delivery via nasogastric tube, intragastric feeding, transpyloric administration and / or any other means of administration that result in the introduction of the nutritional formulation into the digestive tract of a subject.

The invention provides a culture fluid exclusively used in cow in-vitro fertilization embryos. The culture fluid contains 109.0mM-110mM of NaCl, 2.9mM-3.1mM of KCl, 26.0mM-26.5mM of NaHCO3, 0.5mM-1.0mM of MgCl2.6H2O, 1.0mM-1.3mM of KH2PO3, 0.4mM of sodium pyruvate, 1.5 mM of glucose, 5mM of galacturonic calcium, 10v / v% fetal bovine serum, 1mM of L- glutamine, 2v / v% essential amino-acid, 1v / v% nonessential amino acid and 1mM-10mM of glutathione. The cow in-vitro fertilization embryos are placed into the culture fluid to carry out in-vitro cultivation, and results are shown to be obviously superior to those of a control group which is not added with GSH (glutathione), and therefore, developmental rate of blastula and embryo quality are improved, cost for producing embryos in vitro is lowered, experimental basis is provided for applying a cow IVF (in vitro fertilization) technology to practice, and a genetic breeding process can be greatly accelerated.

Compositions and methods for improving the nutritional and physiological status of a woman and her child during all stages of pregnancy are provided herein. This includes pre-conceptional women, pregnant women, and post-natal women (both lactating and non-lactating mothers). The compositions are particularly useful for the neurological, visual, and cognitive development of an embryo, fetus, or infant and the nutritional and physiological well-being of the mother, fetus, and infant. The compositions contain one or more folates, such as a reduced folate and / or folic acid, and one or more essential fatty acids (EFA), such as an omega-3 and / or omega-6 fatty acid. The addition of the essential fatty acid improves upon the folate containing nutritional preparations described in the prior art. The one or more folates and essential fatty acid may be administered together or in separate dosage units. The one or more folates may be selected from folic acid / folate, one or more reduced folates, or a combination of folic acid / folate and one or more reduced folates. The reduced folate is preferably 5-methyltetrahydrofolate, and most preferably 5-methyl-(6S)-tetrahydrofolic acid. The essential fatty acid is preferably an omega-3 fatty acid, and is preferably docosahexenoic acid (DHA) derived from a vegetarian or non-fish source. The compositions may optionally contain other vitamins, minerals, and ingredients, such as, emollient laxatives-all defined herein as “optional or other ingredients”.

The invention discloses functional compound feed special for greenhouse cultivation of litopenaeus vannamei. The feed is prepared from import fishmeal, peanut meal, fermented peanut meal, bean pulp, fermented bean pulp, yeast extract, cuttlefish meal, shrimp shell meal, high protein flour, monocalcium phosphate, granulesten, fish oil, a vitaminized mineral mixture premix compound and a functional additive. The functional additive is prepared from vitamins C, bacillus subtilis, seaweed ultrafine smashing micro powder and natural astaxanthin according to proportion. The feed effectively solves the problems of stress, immunity reduction, variety quality losses and the like caused by greenhouse large-scale intensive cultivation of the litopenaeus vannamei and meanwhile has the advantages of being balanced in nutrition, low in cost, green, environmentally-friendly and the like. Main umami substances in muscles of the litopenaeus vannamei fed through the feed are obviously improved, the total amino acid, necessary total essential amino acid and the like are improved in a certain extent, indexes such as the muscle strength, the freshness and the taste are obviously improved, the shrimp body color is close to that of wild shrimps, the immunity of juvenile shrimps and adult shrimps is obviously improved, and the survival rate is high. The feed is suitable for being used in greenhouse high-density large-scale cultivation of the litopenaeus vannamei.

Pharmaceutical compositions effective in alleviating or reducing the effects of fatigue and weakness associated with cancer and cancer chemotherapy are disclosed. The pharmaceutical compositions of the present invention comprise riboflavin, effectors of the urea cycle in free form or pharmacologically acceptable salts thereof, and amino acids selected from the groups of essential and non-essential amino acids, in free form or pharmaceutically acceptable salts thereof, suitably combined with appropriate carriers, diluents, or excipients. Also disclosed are methods of alleviating or reducing the effects of fatigue and weakness associated with cancer and cancer chemotherapy by administration of pharmaceutical compositions of the present invention.

A composition includes a plurality of amino acids. The plurality of amino acids includes at least one essential amino acid and at least one non-essential amino acid. The plurality of amino acids also includes at least one branch-chain amino acid. The composition also includes a source of carbohydrates. The compositions also includes purified water. The plurality of amino acids comprises about 1 wt % of the composition. A composition includes a plurality of amino acids, sodium citrate, sodium chloride, potassium phosphate, flavoring, a source of carbohydrates, and purified water. In some embodiments of the composition, the plurality of amino acids includes alanine, arginine, aspartate, cystine, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonin, tryptophan, tyrosine, and valine.

A composition for application to the skin can stimulate the in vivo synthesis of collagen and proteoglycans and improve the appearance of the skin, increasing its elasticity and fullness. In general, a composition according to the present invention comprises: (1) an antioxidant compound in a quantity sufficient to enhance collagen synthesis in the skin; (2) an organic penetrant in which the antioxidant compound is soluble in a sufficient quantity that a concentration of the antioxidant compound sufficient to enhance collagen synthesis can be applied topically and penetrate the skin; (3) a mixture of essential amino acids; (4) a supplemental source of sulfur; and (5) a topical pharmaceutically acceptable carrier. The antioxidant compound can be lipoic acid, a lipoic acid analogue or derivative, a bioflavonoid, a constituent of ginkgo, or an isoflavone. The organic penetrant is preferably benzyl alcohol. Other ingredients, such as esters of tocopherol and ascorbic acid, can be included.

The invention relates to a method for preparing an algal biological feed. The method comprises the following steps of: preparing complex microbial inoculants from actinomycetes, saccharomycetes, bacillus subtilis, lactobacillus casei and lactobacillus; and fertilizing algas, and under the synergistic effect of a plurality of strains, generating a mass of cellulase type degraded fibers while synthetising mycoprotein by taking full advantage of an inorganic nitrogen source, an organic nitrogen source and saccharides generated through degradation of the fibers. The algal fermented biological feed provided by the invention has more essential amino-acids and higher nutritive value as compared with algas; animals can absorb the feed quickly and the daily gain can be increased by 4-7%; besides, the immunity of the animals is enhanced, the serum alkaline phosphatase activity is improved by 15% and the alanine transaminase activity is increased by 6%; and the breeding effect is quite obvious. In addition, the preparation method provided by the invention has the advantages of low production cost and short period in contrast with the prior art.

1. An aromatic amino acid derivative represented by the formula (I) or its pharmacologically acceptable salt: wherein, [0001]R1 is a hydrogen atom or an amino-protecting group, [0002]R2 is a halogen atom or an alkyl, aralkyl or aryl group, [0003]R3 is {circle over (1)} a hydrogen atom, {circle over (2)} an aroylamino group, {circle over (3)} a phenyl group substituted with lower alkyl, phenyl, phenoxy, etc. {circle over (4)} a naphthyl or tetrahydronaphthyl group optionally substituted with hydroxy, lower alkoxy or di(lower)alkylamino, {circle over (5)} an unsaturated mono-cyclic heterocyclic group containing N, O and / or S substituted with lower alkyl, phenyl, naphthyl or tetrahydroquinolyl, {circle over (6)} an unsaturated or partially saturated condensed heterocyclic group containing N, O and / or S, optionally substituted with oxo, carboxy, amino, lower alkyl, etc.; X is a halogen atom, an alkyl group or an alkoxy group; Y is oxygen atom or nitrogen atom; I is 0 or 1; m is 0, 1 or 2; n is an integer of 0-5. This compound can inhibit a transporter (LAT1) of essential amino acid which is one of main nutrition for cancer cells and accordingly cause drain of the essential amino acid on the cancer cells and finally can prohibit the multiplication of cancer cells.

Feed additives containing essential amino acids which are diketopiperazines of formulas (IV) or (V) or salts thereof are provided:In formulas (IV) and (V), R1 and R2 may be an amino acid residue such as methionine, lysine, threonine, tryptophan, histidine, valine, leucine, isoleucine, phenylalanine, arginine, and cysteine, and may optionally be the same residue.Additionally provided are the diketopiperazines of formulas (IV) and (V) and a method to for their production.

Disclosed is a pet feed comprising the following ingredients: spirulina 1-5%, fresh beef or chicken 15-30%, bone meal 2-5%, maize flour 30-50%, flour 10-30%, fish meal 5-15%, wheat bran powder 5-15%, yeasts 0.4-1%, table salt 0.7-1.8%, lypressin 0.2-0.8%, and sugar 0.7-1.8%.

The invention provides a culture fluid exclusively used in cow in-vitro fertilization embryos. The culture fluid contains 109.0mM-110mM of NaCl, 2.9mM-3.1mM of KCl, 26.0mM-26.5mM of NaHCO3, 0.5mM-1.0mM of MgCl2.6H2O, 1.0mM-1.3mM of KH2PO3, 0.4mM of sodium pyruvate, 1.5 mM of glucose, 5mM of galacturonic calcium, 10v / v% fetal bovine serum, 1mM of L- glutamine, 2v / v% essential amino-acid, 1v / v% nonessential amino acid and 1mM-10mM of glutathione. The cow in-vitro fertilization embryos are placed into the culture fluid to carry out in-vitro cultivation, and results are shown to be obviously superior to those of a control group which is not added with GSH (glutathione), and therefore, developmental rate of blastula and embryo quality are improved, cost for producing embryos in vitro is lowered, experimental basis is provided for applying a cow IVF (in vitro fertilization) technology to practice, and a genetic breeding process can be greatly accelerated.

Compositions and methods for improving cardiovascular health in the elderly by delivering a selection of essential amino acids, phytosterols, stanols, and isoflavones, which may be supplemented with a low glycemic carbohydrate and / or a medium chain fatty acid.

The present invention provides compositions and methods designed to increase value output of a fermentation reaction that yields a first product, intended for commercialization, such as ethanol, and a fermentation residual used, for example, as animal feed. The methods involve using microorganisms in the fermentation process that have been modified so as to yield a residual having greater value that a residual produced in the process by a microorganism not so modified. In particular, the present invention contemplates using microorganisms in a fermentation process that have been modified to increase production of a nutrient, such as an essential amino acid, thereby reducing the need to supplement the nutrient in the animal's diet. The present invention also provides a modified fermentation residual of higher commercial value. Also provided in the present invention are complete animal feeds, nutritional supplements comprising the subject ferment residuals. Further provided by the present invention is a method of performing fermentation, a modified fermentative microorganism and a genetic vehicle for modifying such microorganism.