118 results about "Yeast Proteins" patented technology

The invention provides a clostridium endophyllum engineering bacterium as well as a construction method and application thereof, and belongs to the technical field of biology, compared with a wild type strain, the clostridium endophyllum engineering bacterium can only produce acetic acid in a low-pH environment under the condition of taking CO2 and H2 as raw materials, and does not generate by-products such as ethanol, lactic acid, 2, 3-butanediol and the like. Besides, the fermentation liquor of the clostridium endophyllum engineering bacteria can be directly used as an acidic substrate to adjust the pH value of downstream yeast fermentation liquor, meanwhile, automatic addition of a carbon source is achieved, zero addition of inorganic salt in the cascade conversion process from CO2 and H2 to grease or yeast protein is achieved, and the economical efficiency and environmental friendliness of the whole process are improved.

The invention provides a preparation method of a composite Pickering emulsion based on modified yeast protein. Comprising the following steps: preparing modified yeast protein through combined treatment of papain and TG enzyme; mixing the modified yeast protein solution with a chitosan solution, and heating at 90 DEG C to obtain a proteoglycan composite solution, wherein the mass ratio of yeast protein to chitosan is (5: 1)-(1: 5); after the proteoglycan composite solution is cooled to room temperature, the pH value is adjusted to 2.5-6.5; 20%-60% of vegetable oil is added into the proteoglycan composite solution, and the Pickering emulsion can be obtained through homogenization. The composite Pickering emulsion based on the modified yeast protein is suitable for complex food matrixes, good in stability and capable of efficiently embedding various functional components and remarkably delaying oxidation, photolysis or thermal degradation of the functional components, so that the product quality is improved, and the shelf life of the product is prolonged. If the monascus pigment is added, the problems of color loss and degradation of the monascus pigment in the application process can also be improved.

The invention relates to the technical field of microorganisms, and provides pichia pastoris CMRC 13Y with high protein yield and application of the pichia pastoris CMRC 13Y. The pichia pastoris CMRC 13Y is separated from naturally-fermented dried milk cakes, the classification name of the pichia pastoris CMRC 13Y is Pichia kudriavzevii, and the preservation number of the pichia pastoris CMRC 13Y is CGMCC No.34655. The pichia pastoris CMRC 13Y has high acid resistance, and the growth of the pichia pastoris CMRC 13Y is not obviously influenced along with the reduction of the pH value within the pH value range of 3.0-7.0; a yeast protein product prepared from the strain CMRC 13Y is subjected to sensory flavor determination, has no earthy smell in the aspect of smell, has fragrance, is fine and smooth in mouth feel, and has no unpleasant taste; the strain can achieve high-biomass growth and high-protein expression, has the advantages of being green, environmentally friendly, high in protein yield and the like, can be used for preparing microbial protein related products and is wide in application prospect.

The invention relates to a Kluyveromyces marxianus strain with high protein yield and application of the Kluyveromyces marxianus strain, which is characterized in that the Kluyveromyces marxianus strain is firstly cultured on a slant, then is transferred into a seed culture medium for seed preparation, and then is inoculated into a fermentation tank, and yeast protein is obtained by adopting index feeding; the bacterial strain is a dominant mutant strain Kluyveromyces marxianus YWX-1, the protein content in a dry thallus reaches 55% or above and is increased by 26% or above compared with that of an original bacterial strain, the bacterial strain can be used for production of single-cell protein, the essential amino acid index (EAAI) value in the protein reaches 1.2, and the bacterial strain is rich in arginine, threonine and lysine and can be applied to production of single-cell protein. These amino acids play a key role in immune regulation, intestinal mucosal barrier function and muscle protein synthesis of young animals. The YWX-1 kluyveromyces marxianus strain has a wide application prospect in the production of neoplasm protein.

The invention relates to the technical field of biology, in particular to a preparation method and application of a yeast protein and polysaccharide compound. According to the method for preparing the yeast protein and polysaccharide compound, the yeast protein and the polysaccharide are compounded, meanwhile, the protein is subjected to enzymolysis, the functional property of the yeast protein is rapidly improved, and meanwhile part of bitter taste and bad flavor are wrapped. The yeast protein and polysaccharide compound prepared through enzymolysis and compounding processes is not prone to sedimentation, the foaming power of the yeast protein prepared through the method reaches 150.00%, the emulsibility reaches 67 m < 2 > / g, the water binding capacity reaches 700%, the yeast protein tastes fine and smooth and smells light, no organic solvent is involved in the production process, and the yeast protein and polysaccharide compound is clean, environmentally friendly and suitable for being used in food systems such as solid beverages and artificial meat.

This invention provides a highly soluble yeast protein and its preparation method, belonging to the field of yeast protein processing technology. In this invention, yeast protein is first dispersed in distilled water and hydrated to prepare a yeast protein solution. The solution is then treated under strongly alkaline conditions and subjected to high-pressure homogenization. The pH of the system is then adjusted back to neutral, and finally, after dialysis and freeze-drying, a highly soluble yeast protein powder is obtained. Compared with commercial yeast protein powder, the yeast protein powder prepared by this method exhibits significantly improved solubility under neutral conditions.

The invention discloses yeast protein nutritional gel and a preparation method thereof, and belongs to the technical field of protein gel. Comprising the following steps: adding dialdehyde starch into a yeast protein solution, then sequentially adding EDC and NHS, fully mixing, and standing to obtain stable gel. The invention has the following technical effects: (1) the formation of a closer molecular network structure between YP and DS can be effectively promoted; (2) the mechanical property of the gel system is obviously improved; (3) an external structure is not introduced, and safety and environmental friendliness are achieved; and (4) rapid reaction can be realized under a mild low-temperature condition.

The invention discloses a sports nutrition solid food for supplementing protein and a preparation method, and belongs to the technical field of foods.The sports nutrition solid food is prepared from, by weight, 31-60 parts of compound amino acid powder, 10-15 parts of isomaltitol, 1-6 parts of taurine, 1-5 parts of Chinese yam peptide, 5 parts of glycine, 5 parts of gamma-aminobutyric acid, 5 parts of L-lysine hydrochloride and 1 part of yeast protein powder. The formula comprises the following components in parts by weight: 1-3 parts of whey protein powder, 1-2 parts of hydroxymethyl propyl cellulose, 0.1-1 part of yeast extract, 0.1-1 part of yeast beta-glucan, 2-6 parts of bovine colostrum powder, 0.5 part of stevioside, 0.1 part of zinc glycinate, 0.1 part of ferrous lactate, 0.5 part of essence and 2-3 parts of magnesium stearate. The compound amino acid powder, the Chinese yam peptide, the glycine, the L-lysine hydrochloride, the yeast protein powder, the whey protein powder, the yeast extract and the bovine colostrum powder are compounded, so that protein consumed by a human body after exercise can be comprehensively supplemented.

The invention relates to a silk fibroin-containing composition as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The composition provided by the invention comprises silk fibroin-tea polyphenol powder, myrothamnus flabellifolia leaf extract, alfalfa extract and hydrolyzed yeast protein, and the silk fibroin-tea polyphenol powder is prepared by grafting two tea polyphenol components, namely EGCG and ECG, onto silk fibroin, so that the original effects of the silk fibroin are retained, and meanwhile, the content of the tea polyphenol components in the silk fibroin-tea polyphenol powder is increased; and the application stability of the silk fibroin in the product can also be improved. Besides, the prepared silk fibroin-tea polyphenol powder, myrothamnus flabellifolia leaf extract, alfalfa extract and hydrolyzed yeast protein are reasonably compounded to prepare the composition, all the components in the composition have a synergistic effect, and the composition has good anti-inflammatory, soothing and barrier repairing effects.

The invention relates to the technical field of microorganism application, in particular to low-nucleic-acid yeast protein as well as a preparation method and application thereof. The preparation method of the low-nucleic-acid yeast protein comprises the following steps: (1) carrying out inactivation treatment on high-protein yeast; (2) carrying out enzymolysis on the inactivated yeast by adopting neutral protease, glucanase, mannase and first nuclease, and after enzyme deactivation, separating and taking a heavy phase to obtain crude yeast protein; (3) performing high-pressure homogenization treatment on the crude yeast protein; and (4) carrying out enzymolysis on the crude yeast protein subjected to high-pressure homogenization treatment by adopting second nuclease and lipase, and after enzyme deactivation, separating and taking a heavy phase to obtain the low-nucleic-acid yeast protein. The yeast protein with the nucleic acid content of 1.0% or below is obtained by taking the high-protein yeast as a raw material through inactivation, compound enzyme enzymolysis, high-pressure homogenization treatment and secondary enzymolysis, is neutral and pure in taste, and can be widely applied to the fields of nutrition, health and food such as protein supplementation and protein substitution.

The invention discloses an anti-inflammatory and anti-aging composition as well as a preparation method and application thereof. The composition disclosed by the invention at least comprises one or more of lavender flower extract, carboxymethyl chitosan and hydrolyzed yeast protein. Compared with a single-component anti-inflammatory factor, the composition disclosed by the invention has a good effect; the product prepared from the composition has anti-inflammatory and anti-aging effects, can achieve the effects of removing redness, repairing skin barrier, increasing skin elasticity, improving skin brightness and improving skin grease, and is not only suitable for dry sensitive skin, but also suitable for oil sensitive skin.

The invention discloses a retinol / saccharomycetes protein polypeptide copolymer as well as a preparation method and application thereof, and relates to the field of biology. The preparation adopting the yeast polypeptide to entrap the retinol is created, the yeast polypeptide is extracted from yeast autolysis supernatant, the yeast autolysis supernatant adopts animal-derived protein as a nitrogen source for fermentation, the content of the retinol entrapped by the yeast polypeptide is remarkably increased, and the preparation is remarkably superior to the retinol in the aspects of stability, mildness, efficacy and the like.

The invention belongs to the technical field of plant meat improvement, and particularly relates to a multi-protein composite high-moisture extruded meat analogue and a fiber strengthening method thereof. Comprising the following components: 40-60 parts of yeast protein, 40-45 parts of soybean protein isolate, 30-40 parts of wheat gluten protein, 1-2 parts of konjaku flour and 150-300 parts of water. The konjac glucomannan is added to enhance the fiber structure of the yeast protein-based plant meat, and the combination of parameters such as temperature during shearing and extrusion and a cooling mold with a special structure and an elliptical cross section are assisted, so that the fiber reinforcement of the multi-protein composite high-moisture extruded meat analogue is realized.

The application discloses a kind of genetic engineering escherichia coli plasmid expression culture medium and fermentation process, including basic medium and feed medium;The basic medium includes glycerol, yeast hydrolysate, yeast peptone, L-glutamic acid, L-valine, L-isoleucine, arginine, tyrosine, lysine, proline, glycine, ammonium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, citric acid, magnesium sulfate, ferrous sulfate, zinc sulfate, calcium chloride, manganese chloride, cobalt chloride, copper chloride, sodium ethylenediaminetetraacetate;Feed medium includes glycerol, citric acid, magnesium sulfate, ferrous sulfate, zinc sulfate, calcium chloride, manganese chloride, cobalt chloride, copper chloride, sodium ethylenediaminetetraacetate.The application inhibits the accumulation of harmful metabolites, and creates the optimal intracellular environment for stable replication of plasmid.In industrial fermentation scale, the amount of plasmid harvested per liter of fermentation broth is more than 1 gram, and the supercoiling ratio of plasmid DNA is stably maintained at more than 90%, with minimal batch-to-batch variation.

The current invention relates to a cheese substitute comprising a vegetable oil and / or fat, starches and / or modified starches, plant-based protein and yeast protein. The invention further relates to a method for preparing the cheese substitute, the use of yeast protein in a cheese substitute and a food product comprising the cheese substitute according to the present disclosure.

A high-purity yeast protein and a preparation method therefor, wherein the method comprises the following steps: inoculating an activated yeast strain into a liquid seed culture medium, culturing same in a shaker, then inoculating same into a fermentation culture medium for culturing, and centrifuging and washing same to obtain a yeast strain; preparing the yeast strain into a suspension solution, and performing a heat treatment to collect a precipitate; preparing the precipitate into a suspension, adding 5-50 U / mL of dextranase and 5-50 U / mL of cellulase at a pH of 5.0-7.0 and 30-60°C, and performing enzymatic hydrolysis to obtain a first enzymatic hydrolysate; adding a non-ionic solubilizer to the first enzymatic hydrolysate at a certain pH, and incubating the resulting mixure at a certain temperature to obtain a second enzymatic hydrolysate; and subjecting the second enzymatic hydrolysate to solid-liquid separation, washing and drying to obtain a high-purity yeast protein. The method reduces the possibility of protein denaturation. The final yeast protein product has a low dietary fiber content, a high branched-chain amino acid content and a high protein content, and exhibits good nutritional value and processing characteristics.

The present invention relates to a transparent hair care composition comprising: 0.1 to 5 wt% hydrolyzed yeast protein, 0.1 to 20wt% a non-ionic surfactant comprising a sorbic acid based non-ionic surfactant and alkoxylated alcohol based non-ionic surfactant, 0.01 to 3 wt% preservative, 0.01 to 3 wt% chelating agent, 0.01 to 3 wt% polycationic polymer, and 80 to 95wt% water.

The invention discloses a low-serum-content culture medium, a low-serum-content inactivated vaccine and a preparation method of the low-serum-content inactivated vaccine, and belongs to the technical field of biological agents. The mycoplasma culture medium with low serum content is prepared from the following components in parts by weight: 94 to 96 parts of culture medium matrix, 4 to 6 parts of animal serum, 0.05 to 0.15 part of yeast peptone CM03 and 0.05 to 0.15 part of L-cysteine. Carrying out first-stage seed culture and second-stage seed culture on the mycoplasma strain to obtain a second-stage seed; carrying out fermentation culture on the secondary seeds by adopting the culture medium to obtain fermentation liquor; and concentrating the fermentation liquor to the target bacterium content, and then inactivating, inspecting and preparing to obtain the inactivated vaccine with low serum content. According to the culture medium, on the basis that the serum content is reduced through reasonable proportioning, the mycoplasma culture efficiency is improved, and the cost and side effects are reduced.

The invention relates to the technical field of dairy product processing, in particular to a yeast protein dairy product and a preparation method thereof.In the yeast protein preparation process, the quality of the yeast protein dairy product can be remarkably improved through the synergistic effect of key processes such as bi-enzyme directional step-by-step hydrolysis, ultrafiltration desalination concentration, compound bacteria liquid fermentation and an emulsification compound system; the yeast dairy product prepared by the method can be further suitable for products such as high-protein beverages, cheese substitutes and stirred yoghurt, and has the advantages of nutrition retention and texture optimization.

The invention belongs to the field of food, and particularly relates to powdered oil containing a yeast source natural emulsifier as well as preparation and application of the powdered oil. The powdered oil is prepared from the following raw materials in parts by weight: 40.0-55.0 parts of vegetable oil, 10.0-30.0 parts of a yeast source natural emulsifier, 0.5-1.0 part of a flow aid, 5.0-40.0 parts of an embedding medium and 5.0-15.0 parts of an auxiliary material, and the yeast source natural emulsifier comprises yeast dietary fiber and / or yeast protein. According to the invention, the yeast protein and the yeast dietary fiber are used as the natural emulsifier of the yeast source to replace an industrially synthesized emulsifier or an emulsifier such as sodium caseinate and the like used for preparing the powdered oil in the prior art, so that not only is the label cleaning of the powdered oil realized, but also the production cost of the powdered oil is effectively reduced.

To provide a cosmetic composition which cleans the skin up.SOLUTION: A cosmetic composition includes soybean protein, whey protein, yeast protein, a koji fermented product and a Sake lees fermented product. The koji fermented product is derived from grains including white rice, barley, red rice, brown rice, millet, common millet, black rice, sorghum and barnyard millet, which are obtained by using white koji mold, black koji mold and yellow koji mold. The cosmetic composition furthermore contains at least one selected from a group consisting of plant-derived lactic acid bacteria derived from Sunki pickles (traditional pickles in the Kiso region in Japan)', soybean extract and glucomannan, Gymnema sylvestre extract and white kidney bean extract, or a wild grass fermented extract.SELECTED DRAWING: None

The present application relates to the field of cosmetic technology, and more particularly to a full-link whitening and freckle-removing liposome and a preparation method thereof, the liposome comprising the following components: an aqueous phase, a membrane material and an oil phase. In the preparation process, 4-butyl resorcinol, nonapeptide-1, acetylchitosamine and ascorbic acid tetraisopalmitate are combined to form a full-link effect of signal inhibition-synthesis blockage-blocked conversion-metabolic acceleration, and a multi-target whitening and freckle-removing system is constructed. In addition, tranexamic acid, hydrolyzed yeast protein and schizophyllan are combined to synergistically make the finished product have excellent soothing and moisturizing effects. Furthermore, the finished product is prepared into a liposome capable of encapsulating active ingredients, which effectively improves the stability of the active ingredients, prevents their oxidation and inactivation, and the liposome structure is highly similar to the human cell membrane, is easy to penetrate the keratin layer gap, realizes targeted delivery to the deep layer of the epidermis, and makes the active ingredients truly act on the required site.

The invention belongs to the technical field of dairy product processing, and discloses yeast protein fermented milk, a preparation method, a device and application, a raw material composition of the fermented milk comprises raw milk, a sweetening agent, yeast protein, a stabilizer and a leavening agent, the yeast protein is compound modified yeast protein, and a dispersion technology combining homogenization and ultrasonic-assisted dispersion is adopted in the preparation process. And a segmented gradient fermentation process and a segmented dynamic after-ripening process are matched. The invention solves the problems of high cost of protein supplement sources, easy generation of peculiar smell, serious whey separation of fermented milk, short shelf life and single nutrition in the prior art, and the obtained product is balanced in nutrition, fine and uniform in texture, pure and harmonious in flavor, obviously prolonged in shelf life, adaptive to industrial production requirements and suitable for industrial production. The method can be applied to production of flavored fermented milk and fermented milk-containing beverages, and has good application value.

The invention belongs to the technical field of yeast protein processing, and discloses a micro-hydrolyzed yeast protein preparation method, which comprises: dissolving with purified water, carrying out heating treatment, removing floating oil, and adsorbing with beta-cyclodextrin; the preparation method comprises the following steps: firstly, performing enzymolysis by using trypsin and flavourzyme in sequence, strictly controlling the addition amount of enzyme and the enzymolysis time, and performing spray drying after enzyme deactivation to obtain the micro-hydrolyzed yeast protein, and discloses the micro-hydrolyzed yeast protein prepared by the method. The preparation method is simple, and the taste and flavor of the obtained product are remarkably improved while the nutritional function of the yeast protein is reserved.

The invention provides a ternary protein textured protein prediction method based on machine learning. The method comprises the following steps: acquiring raw material characteristic parameters, process parameters and product characteristic parameters of three protein raw materials, namely vital gluten, yeast protein and soybean meal under a high-moisture extrusion condition; the method comprises the following steps: constructing a ternary protein coupling characteristic and a yeast protein threshold characteristic on the basis of an inhibition effect of yeast protein on wheat gluten cross-linking and a sensitive characteristic of yeast protein solubility on moisture; constructing a back-propagation neural network model, taking the raw material characteristic parameters, the process parameters, the ternary protein coupling features and the yeast protein threshold features as inputs of the back-propagation neural network model, taking the product characteristic parameters as outputs of the back-propagation neural network model, and performing iterative training on the back-propagation neural network model until convergence to obtain a final product. A textured protein prediction model is obtained; and performing forward quality prediction or reverse process derivation on a to-be-detected system by using the textured protein prediction model.

The present application relates to a kind of anti-wrinkle compositions containing spilanthes extract and its application.The anti-wrinkle composition includes spilanthes extract, acetyl hexapeptide-8, yeast fermentation product filtrate and hydrolyzed yeast protein.Compared with prior art, the anti-wrinkle composition of the present application is compounded with various active ingredients, which can significantly improve the anti-wrinkle effect, repair skin barrier and microecology, increase skin moisture content and reduce transdermal water loss.

The invention relates to the technical field of biological medicine and food fermentation, in particular to a black fungus antihypertensive peptide culture medium composition based on selenium-enriched yeast protein, a liquid fermentation method and antihypertensive application of the black fungus antihypertensive peptide culture medium composition. The culture medium comprises selenium-enriched yeast protein, glucose, fermented soybean water extract dregs, inorganic salt and trace elements, the high-activity antihypertensive peptide is obtained by optimizing a liquid fermentation and separation purification process, the ACE inhibition IC50 value of the high-activity antihypertensive peptide is smaller than 50 micrograms per milliliter, and the high-activity antihypertensive peptide can be compounded with a hawthorn extract and the like to prepare an oral preparation and has a remarkable antihypertensive function.

The invention discloses an aquatic protein feed and a preparation method thereof, and belongs to the technical field of aquaculture feeds, the feed takes enzymolysis hermetia illucens protein, wall-broken yeast protein and enzymolysis pea peptide as a compound protein source, and comprises a multilayer sustained-release microcapsule prepared by adopting a three-fluid electrostatic spraying technology, and probiotics and digestive enzymes are embedded in the multilayer sustained-release microcapsule, so that the aquatic protein feed is prepared. And the outer layer is a pH response type gel and a protective film. The preparation method comprises the following steps: carrying out enzymolysis and membrane separation pretreatment on a compound protein source, constructing the microcapsule by virtue of low-temperature electrostatic spraying, and carrying out low-temperature mixing extrusion and functional post-treatment under the protection of inert gas. According to the invention, time-sequence precise release and efficient protection of nutrients and active ingredients in digestive tracts are realized, the protein digestibility can be obviously improved, the feed coefficient and nitrogen emission are reduced, and the feed has the comprehensive functions of promoting growth, regulating intestinal tracts and enhancing immunity at the same time; the industrial problems that a traditional feed is high in dependence degree on fish meal and low in digestion and utilization rate, and functional additives are prone to inactivation are effectively solved.

The invention relates to the technical field of fermentation, in particular to a protein composition for promoting calcium absorption and muscle synthesis and preparation and application thereof. The protein composition comprises the following components in parts by weight: 0.5-99 parts of yeast protein and 0.5-20 parts of a calcium source, the protein content in the yeast protein is more than or equal to 70%, and the zinc content in the yeast protein is more than or equal to 200mg / kg; in terms of the weight of the yeast protein, the content of serine in the yeast protein is larger than or equal to 50 mg / g, and / or the content of glutamic acid is larger than or equal to 110 mg / g, and / or the content of leucine is larger than or equal to 90 mg / g, and / or the content of isoleucine is larger than or equal to 55 mg / g, and / or the content of valine is larger than or equal to 50 mg / g, and / or the content of lysine is larger than or equal to 94 mg / g. The protein composition prepared by combining the yeast protein as a main raw material with the calcium source and nutritional ingredients such as the vitamin D, the vitamin K, the collagen or the casein phosphopeptides not only can promote absorption and utilization of calcium, but also can promote muscle synthesis and improve muscle quality, so that the requirement of collaborative health of muscles and bones is met.

The present application relates to high protein fermentation technical field, specifically disclose a kind of preparation method for fermenting production of yeast protein based on alcohol waste liquor, to solve the technical problems such as low utilization rate of alcohol waste liquor resource, feed soybean meal supply is nervous and high cost.The method includes the following steps: first, the alcohol waste liquor produced by grain deep processing is concentrated to dry basis content ≥30%, the composite carbon source carrier is crushed, urea and molasses are added to adjust the carbon-nitrogen ratio to (20~25):1, access to the complex fermentation strain containing Candida utilis, solid fermentation is carried out, and the finished product yeast protein is obtained.The present application uses solid fermentation mode, matches composite carbon source carrier to optimize nutrient ratio, step by step controls key process parameters, and the finished product yeast protein is ≥24%, true protein is ≥22%, and nitrogen conversion rate is ≥85%, which can replace soybean meal as feed additive.The method realizes alcohol waste liquor resource utilization, pollution is small, reduces feeding cost, has environmental protection and economic value, and is suitable for industrialization popularization and application.