91 results about "Yeast Proteins" patented technology

The invention provides a clostridium endophyllum engineering bacterium as well as a construction method and application thereof, and belongs to the technical field of biology, compared with a wild type strain, the clostridium endophyllum engineering bacterium can only produce acetic acid in a low-pH environment under the condition of taking CO2 and H2 as raw materials, and does not generate by-products such as ethanol, lactic acid, 2, 3-butanediol and the like. Besides, the fermentation liquor of the clostridium endophyllum engineering bacteria can be directly used as an acidic substrate to adjust the pH value of downstream yeast fermentation liquor, meanwhile, automatic addition of a carbon source is achieved, zero addition of inorganic salt in the cascade conversion process from CO2 and H2 to grease or yeast protein is achieved, and the economical efficiency and environmental friendliness of the whole process are improved.

This invention provides a highly soluble yeast protein and its preparation method, belonging to the field of yeast protein processing technology. In this invention, yeast protein is first dispersed in distilled water and hydrated to prepare a yeast protein solution. The solution is then treated under strongly alkaline conditions and subjected to high-pressure homogenization. The pH of the system is then adjusted back to neutral, and finally, after dialysis and freeze-drying, a highly soluble yeast protein powder is obtained. Compared with commercial yeast protein powder, the yeast protein powder prepared by this method exhibits significantly improved solubility under neutral conditions.

The invention relates to a silk fibroin-containing composition as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The composition provided by the invention comprises silk fibroin-tea polyphenol powder, myrothamnus flabellifolia leaf extract, alfalfa extract and hydrolyzed yeast protein, and the silk fibroin-tea polyphenol powder is prepared by grafting two tea polyphenol components, namely EGCG and ECG, onto silk fibroin, so that the original effects of the silk fibroin are retained, and meanwhile, the content of the tea polyphenol components in the silk fibroin-tea polyphenol powder is increased; and the application stability of the silk fibroin in the product can also be improved. Besides, the prepared silk fibroin-tea polyphenol powder, myrothamnus flabellifolia leaf extract, alfalfa extract and hydrolyzed yeast protein are reasonably compounded to prepare the composition, all the components in the composition have a synergistic effect, and the composition has good anti-inflammatory, soothing and barrier repairing effects.

The invention belongs to the technical field of plant meat improvement, and particularly relates to a multi-protein composite high-moisture extruded meat analogue and a fiber strengthening method thereof. Comprising the following components: 40-60 parts of yeast protein, 40-45 parts of soybean protein isolate, 30-40 parts of wheat gluten protein, 1-2 parts of konjaku flour and 150-300 parts of water. The konjac glucomannan is added to enhance the fiber structure of the yeast protein-based plant meat, and the combination of parameters such as temperature during shearing and extrusion and a cooling mold with a special structure and an elliptical cross section are assisted, so that the fiber reinforcement of the multi-protein composite high-moisture extruded meat analogue is realized.

The application discloses a kind of genetic engineering escherichia coli plasmid expression culture medium and fermentation process, including basic medium and feed medium;The basic medium includes glycerol, yeast hydrolysate, yeast peptone, L-glutamic acid, L-valine, L-isoleucine, arginine, tyrosine, lysine, proline, glycine, ammonium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, citric acid, magnesium sulfate, ferrous sulfate, zinc sulfate, calcium chloride, manganese chloride, cobalt chloride, copper chloride, sodium ethylenediaminetetraacetate;Feed medium includes glycerol, citric acid, magnesium sulfate, ferrous sulfate, zinc sulfate, calcium chloride, manganese chloride, cobalt chloride, copper chloride, sodium ethylenediaminetetraacetate.The application inhibits the accumulation of harmful metabolites, and creates the optimal intracellular environment for stable replication of plasmid.In industrial fermentation scale, the amount of plasmid harvested per liter of fermentation broth is more than 1 gram, and the supercoiling ratio of plasmid DNA is stably maintained at more than 90%, with minimal batch-to-batch variation.

The current invention relates to a cheese substitute comprising a vegetable oil and / or fat, starches and / or modified starches, plant-based protein and yeast protein. The invention further relates to a method for preparing the cheese substitute, the use of yeast protein in a cheese substitute and a food product comprising the cheese substitute according to the present disclosure.

A high-purity yeast protein and a preparation method therefor, wherein the method comprises the following steps: inoculating an activated yeast strain into a liquid seed culture medium, culturing same in a shaker, then inoculating same into a fermentation culture medium for culturing, and centrifuging and washing same to obtain a yeast strain; preparing the yeast strain into a suspension solution, and performing a heat treatment to collect a precipitate; preparing the precipitate into a suspension, adding 5-50 U / mL of dextranase and 5-50 U / mL of cellulase at a pH of 5.0-7.0 and 30-60°C, and performing enzymatic hydrolysis to obtain a first enzymatic hydrolysate; adding a non-ionic solubilizer to the first enzymatic hydrolysate at a certain pH, and incubating the resulting mixure at a certain temperature to obtain a second enzymatic hydrolysate; and subjecting the second enzymatic hydrolysate to solid-liquid separation, washing and drying to obtain a high-purity yeast protein. The method reduces the possibility of protein denaturation. The final yeast protein product has a low dietary fiber content, a high branched-chain amino acid content and a high protein content, and exhibits good nutritional value and processing characteristics.

The invention discloses a low-serum-content culture medium, a low-serum-content inactivated vaccine and a preparation method of the low-serum-content inactivated vaccine, and belongs to the technical field of biological agents. The mycoplasma culture medium with low serum content is prepared from the following components in parts by weight: 94 to 96 parts of culture medium matrix, 4 to 6 parts of animal serum, 0.05 to 0.15 part of yeast peptone CM03 and 0.05 to 0.15 part of L-cysteine. Carrying out first-stage seed culture and second-stage seed culture on the mycoplasma strain to obtain a second-stage seed; carrying out fermentation culture on the secondary seeds by adopting the culture medium to obtain fermentation liquor; and concentrating the fermentation liquor to the target bacterium content, and then inactivating, inspecting and preparing to obtain the inactivated vaccine with low serum content. According to the culture medium, on the basis that the serum content is reduced through reasonable proportioning, the mycoplasma culture efficiency is improved, and the cost and side effects are reduced.

The invention relates to the technical field of dairy product processing, in particular to a yeast protein dairy product and a preparation method thereof.In the yeast protein preparation process, the quality of the yeast protein dairy product can be remarkably improved through the synergistic effect of key processes such as bi-enzyme directional step-by-step hydrolysis, ultrafiltration desalination concentration, compound bacteria liquid fermentation and an emulsification compound system; the yeast dairy product prepared by the method can be further suitable for products such as high-protein beverages, cheese substitutes and stirred yoghurt, and has the advantages of nutrition retention and texture optimization.

The invention belongs to the field of food, and particularly relates to powdered oil containing a yeast source natural emulsifier as well as preparation and application of the powdered oil. The powdered oil is prepared from the following raw materials in parts by weight: 40.0-55.0 parts of vegetable oil, 10.0-30.0 parts of a yeast source natural emulsifier, 0.5-1.0 part of a flow aid, 5.0-40.0 parts of an embedding medium and 5.0-15.0 parts of an auxiliary material, and the yeast source natural emulsifier comprises yeast dietary fiber and / or yeast protein. According to the invention, the yeast protein and the yeast dietary fiber are used as the natural emulsifier of the yeast source to replace an industrially synthesized emulsifier or an emulsifier such as sodium caseinate and the like used for preparing the powdered oil in the prior art, so that not only is the label cleaning of the powdered oil realized, but also the production cost of the powdered oil is effectively reduced.

The present application relates to the field of cosmetic technology, and more particularly to a full-link whitening and freckle-removing liposome and a preparation method thereof, the liposome comprising the following components: an aqueous phase, a membrane material and an oil phase. In the preparation process, 4-butyl resorcinol, nonapeptide-1, acetylchitosamine and ascorbic acid tetraisopalmitate are combined to form a full-link effect of signal inhibition-synthesis blockage-blocked conversion-metabolic acceleration, and a multi-target whitening and freckle-removing system is constructed. In addition, tranexamic acid, hydrolyzed yeast protein and schizophyllan are combined to synergistically make the finished product have excellent soothing and moisturizing effects. Furthermore, the finished product is prepared into a liposome capable of encapsulating active ingredients, which effectively improves the stability of the active ingredients, prevents their oxidation and inactivation, and the liposome structure is highly similar to the human cell membrane, is easy to penetrate the keratin layer gap, realizes targeted delivery to the deep layer of the epidermis, and makes the active ingredients truly act on the required site.

The invention belongs to the technical field of dairy product processing, and discloses yeast protein fermented milk, a preparation method, a device and application, a raw material composition of the fermented milk comprises raw milk, a sweetening agent, yeast protein, a stabilizer and a leavening agent, the yeast protein is compound modified yeast protein, and a dispersion technology combining homogenization and ultrasonic-assisted dispersion is adopted in the preparation process. And a segmented gradient fermentation process and a segmented dynamic after-ripening process are matched. The invention solves the problems of high cost of protein supplement sources, easy generation of peculiar smell, serious whey separation of fermented milk, short shelf life and single nutrition in the prior art, and the obtained product is balanced in nutrition, fine and uniform in texture, pure and harmonious in flavor, obviously prolonged in shelf life, adaptive to industrial production requirements and suitable for industrial production. The method can be applied to production of flavored fermented milk and fermented milk-containing beverages, and has good application value.

The invention belongs to the technical field of yeast protein processing, and discloses a micro-hydrolyzed yeast protein preparation method, which comprises: dissolving with purified water, carrying out heating treatment, removing floating oil, and adsorbing with beta-cyclodextrin; the preparation method comprises the following steps: firstly, performing enzymolysis by using trypsin and flavourzyme in sequence, strictly controlling the addition amount of enzyme and the enzymolysis time, and performing spray drying after enzyme deactivation to obtain the micro-hydrolyzed yeast protein, and discloses the micro-hydrolyzed yeast protein prepared by the method. The preparation method is simple, and the taste and flavor of the obtained product are remarkably improved while the nutritional function of the yeast protein is reserved.

The invention provides a ternary protein textured protein prediction method based on machine learning. The method comprises the following steps: acquiring raw material characteristic parameters, process parameters and product characteristic parameters of three protein raw materials, namely vital gluten, yeast protein and soybean meal under a high-moisture extrusion condition; the method comprises the following steps: constructing a ternary protein coupling characteristic and a yeast protein threshold characteristic on the basis of an inhibition effect of yeast protein on wheat gluten cross-linking and a sensitive characteristic of yeast protein solubility on moisture; constructing a back-propagation neural network model, taking the raw material characteristic parameters, the process parameters, the ternary protein coupling features and the yeast protein threshold features as inputs of the back-propagation neural network model, taking the product characteristic parameters as outputs of the back-propagation neural network model, and performing iterative training on the back-propagation neural network model until convergence to obtain a final product. A textured protein prediction model is obtained; and performing forward quality prediction or reverse process derivation on a to-be-detected system by using the textured protein prediction model.

The present application relates to a kind of anti-wrinkle compositions containing spilanthes extract and its application.The anti-wrinkle composition includes spilanthes extract, acetyl hexapeptide-8, yeast fermentation product filtrate and hydrolyzed yeast protein.Compared with prior art, the anti-wrinkle composition of the present application is compounded with various active ingredients, which can significantly improve the anti-wrinkle effect, repair skin barrier and microecology, increase skin moisture content and reduce transdermal water loss.

The invention relates to the technical field of biological medicine and food fermentation, in particular to a black fungus antihypertensive peptide culture medium composition based on selenium-enriched yeast protein, a liquid fermentation method and antihypertensive application of the black fungus antihypertensive peptide culture medium composition. The culture medium comprises selenium-enriched yeast protein, glucose, fermented soybean water extract dregs, inorganic salt and trace elements, the high-activity antihypertensive peptide is obtained by optimizing a liquid fermentation and separation purification process, the ACE inhibition IC50 value of the high-activity antihypertensive peptide is smaller than 50 micrograms per milliliter, and the high-activity antihypertensive peptide can be compounded with a hawthorn extract and the like to prepare an oral preparation and has a remarkable antihypertensive function.

The invention discloses an aquatic protein feed and a preparation method thereof, and belongs to the technical field of aquaculture feeds, the feed takes enzymolysis hermetia illucens protein, wall-broken yeast protein and enzymolysis pea peptide as a compound protein source, and comprises a multilayer sustained-release microcapsule prepared by adopting a three-fluid electrostatic spraying technology, and probiotics and digestive enzymes are embedded in the multilayer sustained-release microcapsule, so that the aquatic protein feed is prepared. And the outer layer is a pH response type gel and a protective film. The preparation method comprises the following steps: carrying out enzymolysis and membrane separation pretreatment on a compound protein source, constructing the microcapsule by virtue of low-temperature electrostatic spraying, and carrying out low-temperature mixing extrusion and functional post-treatment under the protection of inert gas. According to the invention, time-sequence precise release and efficient protection of nutrients and active ingredients in digestive tracts are realized, the protein digestibility can be obviously improved, the feed coefficient and nitrogen emission are reduced, and the feed has the comprehensive functions of promoting growth, regulating intestinal tracts and enhancing immunity at the same time; the industrial problems that a traditional feed is high in dependence degree on fish meal and low in digestion and utilization rate, and functional additives are prone to inactivation are effectively solved.

The invention relates to the technical field of fermentation, in particular to a protein composition for promoting calcium absorption and muscle synthesis and preparation and application thereof. The protein composition comprises the following components in parts by weight: 0.5-99 parts of yeast protein and 0.5-20 parts of a calcium source, the protein content in the yeast protein is more than or equal to 70%, and the zinc content in the yeast protein is more than or equal to 200mg / kg; in terms of the weight of the yeast protein, the content of serine in the yeast protein is larger than or equal to 50 mg / g, and / or the content of glutamic acid is larger than or equal to 110 mg / g, and / or the content of leucine is larger than or equal to 90 mg / g, and / or the content of isoleucine is larger than or equal to 55 mg / g, and / or the content of valine is larger than or equal to 50 mg / g, and / or the content of lysine is larger than or equal to 94 mg / g. The protein composition prepared by combining the yeast protein as a main raw material with the calcium source and nutritional ingredients such as the vitamin D, the vitamin K, the collagen or the casein phosphopeptides not only can promote absorption and utilization of calcium, but also can promote muscle synthesis and improve muscle quality, so that the requirement of collaborative health of muscles and bones is met.

The present application relates to high protein fermentation technical field, specifically disclose a kind of preparation method for fermenting production of yeast protein based on alcohol waste liquor, to solve the technical problems such as low utilization rate of alcohol waste liquor resource, feed soybean meal supply is nervous and high cost.The method includes the following steps: first, the alcohol waste liquor produced by grain deep processing is concentrated to dry basis content ≥30%, the composite carbon source carrier is crushed, urea and molasses are added to adjust the carbon-nitrogen ratio to (20~25):1, access to the complex fermentation strain containing Candida utilis, solid fermentation is carried out, and the finished product yeast protein is obtained.The present application uses solid fermentation mode, matches composite carbon source carrier to optimize nutrient ratio, step by step controls key process parameters, and the finished product yeast protein is ≥24%, true protein is ≥22%, and nitrogen conversion rate is ≥85%, which can replace soybean meal as feed additive.The method realizes alcohol waste liquor resource utilization, pollution is small, reduces feeding cost, has environmental protection and economic value, and is suitable for industrialization popularization and application.

This invention belongs to the field of biochemical processing technology, and specifically relates to a yeast protein with high emulsifying properties and emulsion stability, and its preparation method. The invention first employs ultrasound-assisted pH induction to pretreat the yeast protein, utilizing a physical field to disrupt the protein's aggregation state and increase the reaction interface. Simultaneously, the pH induction treatment further ensures the protein's complete unfolding, exposing the maximum number of glycosylation reaction sites such as amino and carboxyl groups, and releasing more hydrophobic groups from the yeast protein. Finally, the pretreated yeast protein is glycosylated using high-molecular-weight polysaccharides based on high temperature and / or TG enzymatic hydrolysis. The resulting yeast protein exhibits significantly improved emulsifying properties and emulsion stability.

The invention discloses a medium-temperature organic acid inducible high-expression promoter for yeast and application thereof. The promoter has a high transcriptional activity promoter in yeast and a high expression promoter which can be induced by succinic acid, citric acid and fumaric acid under a medium temperature condition. The promoter provided by the invention can be used for enhancing target gene expression and providing a promoter element for yeast synthetic biology application, so that for example, the expression capability of yeast protein can be improved.

The invention discloses an online detection method for protein content and / or biomass in a yeast fermentation process. The method comprises the following steps: in a yeast fermentation process, collecting near infrared spectrum data of yeast in a fermentation container or a bioreactor on line in situ by adopting a spectrograph through an optical fiber probe, and obtaining a sample corresponding to the spectrum data; determining the protein content and / or biomass by adopting a standard method; enabling the near infrared spectrum data to be in one-to-one correspondence with the measured protein content and / or biomass, and establishing a sample database; performing preprocessing, dimension reduction and / or feature extraction on the spectral data; and based on a training set sample database, establishing a protein content and / or biomass detection model of the yeast by adopting a regression modeling algorithm, and performing verification and optimization by utilizing data in the verification set according to a preset precision evaluation requirement. The method is simple and efficient to operate, does not damage samples, does not need expensive instruments, can realize real-time detection of multiple parameters in the yeast fermentation process, and provides technical support for large-scale biological manufacturing.

The present application provides a use of a citrus peel ferment for preparing a fat-reducing composition. The citrus peel ferment is prepared by adding a peel of a citrus fruit to a yeast culture and fermenting at 25°C to 35°C for 3 to 10 days; wherein the yeast culture is obtained by adding 0.01% to 0.5% w / v of Saccharomyces cerevisiae to a yeast culture solution and culturing at 25°C to 35°C for 3 to 5 days; wherein the yeast culture solution is an aqueous solution comprising 1% to 5% w / v of yeast protein hydrolysate and 10% to 15% (w / v) of glucose. Furthermore, the prepared citrus peel ferment can assist obese people in losing weight through various mechanisms, such as reducing the fat content of fat cells, promoting the decomposition of triglycerides by fat cells, and increasing the expression of genes related to fat metabolism.

The invention relates to liquid compositions, preferably liquid emulsions which comprise a fat component, at least one non-proteic thickening agent and a yeast protein source. The yeast protein source comprises at least 40wt.% yeast proteins. The total protein content of the liquid compositions, preferably liquid emulsions is 0.5 to 5wt.%. It also relates to powder compositions, aerated compositions and products comprising yeast proteins. It also relates to use and method involving said liquid compositions, powder compositions, aerated compositions and products. It also relates to processes for preparing liquid compositions, powder compositions and aerated compositions.

The invention discloses edible fresh-keeping gel beads for a vegetable protein beverage and a preparation method of the edible fresh-keeping gel beads, and belongs to the technical field of functional food and intelligent packaging. According to the edible fresh-keeping gel beads prepared by the preparation method disclosed by the invention, Schiff base formed by perillaldehyde-yeast protein is used as a liquid core, and Ca < 2 + > is used for pre-crosslinking; the wall material is a sodium alginate-rice protein composite network and can intelligently respond to imine bond protonation caused by pH drop in the beverage storage process, so that controllable release of perillaldehyde is realized, protein aggregation and microbial growth are inhibited, and a clean label and original sense are kept. According to the invention, triple advantages of edible, intelligent response and low cost are taken into consideration, and a plug-and-play fresh-keeping solution is provided for the plant-based protein beverage.

The application relates to a biota sesquiterpene synthase PoTPS1 and a coding gene thereof, the full length of the gene is 1746 bp, the gene codes 582 amino acids, the molecular weight of the enzyme is 67.9 kDa, a pSP-GM1-URA yeast protein expression plasmid is used as a carrier, saccharomyces cerevisiae CEN.PK-5D-113 is used as a host, the biota sesquiterpene synthase PoTPS1 is realized in the heterologous expression of eukaryotic cells, and two kinds of beta-himachalene and cedrol sesquiterpene alcohols can be generated at the same time; a pET22b escherichia coli protein expression plasmid is used as a carrier, anti-freezing protein XXA is added as a fusion tag, escherichia coli BL21 (DE3) is used as a host, the biota sesquiterpene synthase PoTPS1 is realized in the heterologous expression of prokaryotic cells, and a sesquiterpene compound is generated by taking ammonium salt FPP as a substrate.

The invention belongs to the field of food, and particularly relates to a yeast enzymatic hydrolysate, a yeast protein and a yeast protein sodium salt product with high emulsifying performance as well as preparation and application of the yeast enzymatic hydrolysate and the yeast protein sodium salt product. The emulsifying activity of the yeast enzymatic hydrolysate is greater than or equal to 25.0 m / g, and the emulsifying stability is greater than or equal to 77.0%; the emulsifying activity of the yeast protein is greater than or equal to 70.0 m / g, and the emulsifying stability is greater than or equal to 175.0%; the emulsifying activity of the yeast protein is greater than or equal to 70.0 m / g, and the emulsifying stability is greater than or equal to 175.0%. According to the preparation method disclosed by the invention, the yeast is subjected to composite enzymolysis through the alkaline protease, the flavourzyme, the glucanase, the mannase and the glutamine transaminase and then is subjected to ionization reaction with the sodium hydroxide to obtain the yeast protein sodium salt product, and the yeast protein sodium salt product has relatively high emulsifying activity and also has relatively good color and flavor; the sodium caseinate can be used as a natural emulsifier, and the problems of limited sodium caseinate yield, unstable supply and high price are solved.

The present invention relates to the medical technical field, specifically to a yeast protein, its composition, and its manufacturing method and use. The yeast protein of the present invention is spherical or elliptical, with the outside wrapped in zymosan, and based on the dry weight percentage, the yeast protein has a protein content of 70% or more and a zymosan content of 5-30%. The yeast protein of the present invention has a gastric digestibility that is obviously lower than soy protein isolate and whey protein, and the postprandial satiety is greater than that of whey protein, and has the characteristic of being slowly digested, so it is a slowly digestible protein. In addition, the yeast protein of the present invention contains 18 kinds of amino acids, including 8 kinds of amino acids essential for the human body and 2 kinds of semi-essential amino acids, and has a complete amino acid variety, so it belongs to a complete protein. As a slowly digestible protein, it can be used in the manufacture of a composition for enterally administering to a mammal to adjust postprandial plasma amino acid levels.

Disclosed herein is a plant-based, blood-free diet formulation for hematophagous insects comprising effective amounts of a plant-based protein source and, in some aspects, a carbohydrate source, wherein the plant-based, blood-free diet formulation is suitable for egg production and colony maintenance of hematophagous insects. In some aspects, such hematophagous insects include, but are not limited to, mosquitoes. In some aspects, the plant-based protein source is derived from hemp seeds. In some aspects, the protein source comprises additional proteins in addition to hemp protein. In some aspects, one or more additional proteins in the formulation is derived from spirulina, soybeans, chia seeds, quinoa, oats, nutritional yeast, and / or green peas.

The invention discloses a method for enhancing the synthetic ability of yeast total protein and dipeptide tripeptide. Relates to the technical field of synthetic biology and metabolic engineering. According to the invention, the saccharomyces cerevisiae engineering strain with high protein synthesis capability and high endogenous dipeptide and tripeptide accumulation level is successfully constructed. The metabonomics analysis reveals the unique peptide fragment accumulation spectrum, especially the generation of a large amount of lysine-rich tripeptide at the C terminal, and proposes a potential mechanism of metabolic pathway'bottleneck 'caused by peptidase activity down-regulation in combination with proteome data. The recombinant strain has important application potential in the fields of single-cell protein, functional peptide products and the like.