The invention relates to a method for detecting activity of 
catalase, belonging to the field of enzymatic 
activity detection methods. The method for detecting activity of 
catalase comprises the following steps: diluting a 
catalase standard substance into a 
standard solution with a series of catalase activity concentrations, adding 10mL of 
chlorine peroxide phosphate buffer solution in a 100mL 
iodine measuring flask, putting the 
iodine measuring flask in water bath at 25 DEG C and carrying out heat preservation, then adding 2mL of a standard 
enzyme solution, accurately carrying out heat preservation for 3 minutes, instantly adding 2mL of 0.5mol / L 
sulfuric acid to end reaction, adding 2mL of 0.5mol / L 
sulfuric acid into a contrast tube of a contrast reaction solution to end reaction, and then adding the 
enzyme solution; then extracting 0.1mL of the reaction solution, adding 2.9mL of a 
peroxidase-dianisidine solution in a 
test tube and shaking to be uniform, firstly carrying out zero adjustment by a blank 
test tube in 436microns by virtue of a spectrophotometer at 25 DEG C, then detecting absorbancy of each reaction solution 
test tube to obtain a curve about the relation between the standard enzymatic activity and the absorbancy. The test 
enzyme is processed by the same method, so that the enzymatic activity of the test enzyme can be obtained according to the absorbancy and the 
standard curve. The detection method has the characteristics of being accurate, high in practicability, simple in instrument requirement and suitable for operation in the conventional condition; the detection method belongs to one reliable method of color rendering methods for detecting the activity of the catalase.