61 results about "Birth rate" patented technology

The invention provides a rex rabbit feed. The raw materials of the rex rabbit feed are easily obtained; the rex rabbit feed is rich in multiple nutritional components; the appetite of a rex rabbit can be improved so that the rex rabbit likes to eat; the rex rabbit grows with high speed after eating the feed; a great amount of good furs and leathers can be obtained; the cost of feed for breeding rex rabbits is reduced; the immune function of the rex rabbit is enhanced; and the disease prevention, disease curing and anti-virus capacities of the rex rabbit are improved so that the rex rabbit has no need of taking antibiotics drugs. Therefore, no chemical drug residue exists; meanwhile, the breed fertility of a rex rabbit can be effectively improved; the birth rate of a pregnant rex rabbit is increased; the newborn litter weight is increased; the still birth rate is reduced; and the weanling number and the weaning litter weight of a lactational female rex rabbit are increased.

The invention belongs to the technical field of pig feed production, and in particular relates to a pig feed for promoting the oestrus of sows and improving the birth rate of piglets. The feed has the advantages that the sows are rapid in oestrus, short in period and multiple in piglets. The pig feed is composed of a conventional feed which promotes the oestrus of the sows and improves the birth rate of the piglets and Chinese herbal medicines, wherein the conventional feed consists of the raw materials of corns, bean pulp, extruded soybeans, import fish meal, corn gluten meal, peanut meal, bran, soybean oil, calcium hydrophosphate, mountain flour, boar multi-vitamins, multiple minerals, lysine, choline chloride and cysteamine; the Chinese herbal medicines consist of the raw materials of herba epimedii, actinolite, leonurus, Chinese angelica, semen cuscutae and nutgrass, and in the conventional feed, the addition amount of the Chinese herbal medicines accounts for 2.0-4.0% of the total amount of the feed. According to the feed, the medicine properties of the Chinese herbal medicines are utilized on the basis that the formula of the main ingredients is optimized, thereby improving the reproduction function of the sows, promoting the early oestrus of the sows and the birth of the multiple piglets, improving the reproduction capacity and the production property of the sows.

The invention discloses a method for artificially breeding, reproducing and releasing dace (leuciscus brandti), belongs to the field of artificial breeding, reproduction and releasing of fish and relates to a method for artificially breeding, reproducing and releasing fish. To solve the technical problem that the incubation rate and the survival rate are low, the method comprises the first step of catching the parent dace (leuciscus brandti), the second step of collecting, fertilizing and de-bonding eggs of the dace (leuciscus brandti), the third step of incubating fertilized eggs and rearing fry, and the fourth step of releasing the fry. By the adoption of the mode of aeration at the bottom of an incubation tank, dissolved oxygen during incubation of the fertilized eggs is increased; besides, due to the aeration, the viscosity of the fertilized eggs is lowered, and therefore the defects that in the prior art, the incubation rate and the survival rate are low can be effectively overcome. By adopting the method, the average daily incubation rate can reach over 80 percent, the birth rate of the fry can reach over 90 percent, and the survival rate can reach 95 percent.

The invention relates to a culture method for improving embryonic birth rate of crops and particularly relates to a culture method for improving the embryonic birth rate of cabbage stalk, belonging to the technical field of plant culture. According to the culture method disclosed by the invention, a microspore culture technology is utilized for co-culture of the cabbage stalk and rapeseed microspores, so that the germ extraction rate of the cabbage stalk can be significantly improved and the problem of low embryogenesis frequency of microspore culture of the cabbage stalk is further solved.

The present invention discloses a vector, a cell and a method for improving bovine cloning efficiency on the basis of histone methylation modifying level. The vector includes a repressor expression vector and a repressor-operon-containing expression vector, and in the transfection, the repressor expression vector and the repressor-operon-containing expression vector are cotransfected into donor cells; and bovine KDM4B or bovine KDM4C gene is inserted into the repressor-operon-containing expression vector. Positive transfected cells as donor cells are injected into enucleated oocytes for electro-fusion, and successfully-fused bovine somatic cell cloned embryos are selected for culturing for 36h for induced expression of the bovine KDM4B and bovine KDM4C. Histone H3K9me3 epigenetic modifying level of the somatic cell cloned embryos in zygotic activation period can be effectively reduced, the development and quality of post-bovine somatic cell cloned blastocysts can be significantly improved, efficient and in-vitro production of the bovine somatic cell cloned embryos can be achieved, and birth rate of cloned cattle after embryo transplant can be significantly increased.

The invention belongs to the technical field of animal husbandry, and particularly relates to a method for enhancing birth efficiency of lambs during lactation through standardized feeding management of the ewe and the lambs. The method comprises the following steps: promoting the ewes enter the oestrus period at the same time, and intensively mating; manually separating the ewes and the lambs after the ewes give birth to lambs, weaning the ewes, promoting the ewes enter the oestrus period again, and finishing the lactation of the ewes; and manually feeding the lambs with colostrum, feeding the lambs with common milk after the lambs defecate, then gradually exchanging the common milk with fine feed or granulated feed to feed the lambs, and finally feeding the lambs with weeds after 3 to 4months to make the lambs enter the fattening period. Aiming at the problem of low birth rate of sheep cultivating industry, the invention provides an integral lactation lamb birth technology integrating the ewe matting, the lamb birth, the colostrum (common milk) distribution, the ewe weaning and the lamb feeding planning. The method provided by the invention enhances the birth capability of the lamb, shortens the breeding cycle of the ewes, and scientifically solves the actual problems of the large-scale sheep birth at the same time, thereby having a wide application prospect.

The invention discloses a conveying path combination optimization method based on a stage structured predator-prey model. Supposing that multiple animal populations grow in an ecological system, and each population is divided into two stage types of adult populations and young populations; the adult populations produce their young populations at a certain birth rate, and the young populations grow up into the adult populations after a period of time; the influence of mutual competition for living resources between the populations is shown in the influence on the characteristics; the population having larger proportion in all the populations has higher influence, and the population spreads its influence to other populations; the advantageous and strong populations spread their high quality characteristics to other populations; and if one population is continuously influenced by other populations, its growth state continuously changes, and the global optimal solution of the problem of conveying path combination optimization can be rapidly determined by using the change and the multi-population predator-prey system model having the stage structure.

The invention discloses a yearly artificial propagation method of mandarin fish. With the adoption of an ecological and physiological adjustment method, baby fish of mandarin fish are born every 20-30days, thus shortening propagation period and greatly increasing yearly birth rate. Since baby fish can be utilized frequently, boby fish can breed 9-12 times, thus improving the utilization rate andartificial propagation efficiency of baby fish, reducing the stock of baby fish and the cost used to cultivate baby fish, and increasing the ecnomic benefits by 6 times and above. According to the propagation method disclosed by the invention, the spawning rate, the fertility rate and the hatching rate of baby fish are high; the quality of fry is good; fry reaching sales specifications of 3 cm whole length are obtained after being cultivated for 5-20 days; the economic benefits of farmers are increased; artificial propagation of mandarin fish is no affected or limited by seasons or time; the long-standing problem of early releasing of fry in cold springs and insufficient fry out of season is solved; fry varieties are supplied year in year out; the purpose of balanced production is achieved; and mass production of mandarin fish fry can be facilitated.

Described herein are methods and compositions for modulating bovine birth rate by following a breeding scheme based on the presence of the trio haplotype, which is strongly linked to the propensity to give birth to multiple calves in one event.

The invention discloses a method for application of H3K27me3 and demethylase KDM6A / B thereof to a nuclear transfer reconstructed embryo of a mouse. With the application method, the difference between an H3K27 trimethylation mode in a parthenogenetic embryo in each period before implantation and that in a clonal embryo, and the influence of the difference in the mode on the developmental capacity of the clonal embryo are analyzed, and the method verifies that fine adjustment of KDM6A and KDM6B can improve the blastocyst development rate of the cloned embryo and the birth rate of a cloned animal.

The invention discloses a cultivation method of a seahorse artificial breeding broodstock. The method is characterized in comprising the following steps: (1) broodstock selection is carried out, wherein artificially cultivated robust and lively seahorses with clean surfaces, large sizes and high intake ability are adopted as an artificial breeding seahorse broodstock; (2) broodstock temporary keeping is carried out, wherein selected seahorses are temporarily kept in an indoor broodstock tank; chilled or live mysids are used for feeding, wherein the seahorses are fed 4-5 times a day. Temporary keeping conditions are that environment salinity is 24-25, temperature is 24-26 DEG C, white light or blue light with light intensity of 40*10<2> Lx is adopted, a background sound is 30-40dB, a water depth is 70-75cm, attachments are natural plants, and sand-filtered seawater is adopted. Male and female seahorse broodstocks are kept isolated, wherein a breeding density is 2 males and 2 females. When seahorse body color is changed and mating behavior appears, the female and male seahorses are mixed. With the method, an optimal artificial cultivation effect can be obtained, broodstock seahorse birthing rate can be improved, and baby seahorse survival rate can be improved.

The invention discloses an optimized mice somatic cell nuclear transplantation method. According to the mice somatic cell nuclear transplantation method, three kinds of cells, including cumulus cell, fetal fibroblasts, and embryonic stem cell, are adopted to construct reconstructed embryos; the activation efficiencies of three reconstructed embryos in different activation mediums are compared, development efficiencies in different solutions are compared, and pregnancy efficiencies of different embryo transplantation methods are compared; it is concluded that calcium-free KSOM activation medium is a universal high efficiency activation medium, and the activation efficiency is about 93.5%; KSOM-AA culture medium is suitable for in vitro culture of the reconstructed embryos of cumulus cells and embryonic stem cells, and the best culture medium for fetal fibroblasts is alpha MEM culture medium; when the reconstructed embryos are at 2-cell period, nuclear transplanted animals can be obtained via transplantation of embryos through bilateral fallopian tubes, wherein for each fallopian tube, transplantation of 15 embryos is carried out. The optimized mice somatic cell nuclear transplantation method is capable of increasing reconstructed embryo cleavage rate, blastula development rate, cloning animal birth rate, and survival rate, so that the success rate of obtaining embryonic stem cells from the reconstructed embryos is increased.

The invention relates to a breeding method for pigs, in particular to an artificial fertilization method for pigs, and belongs to the technical field of breeding. The method comprises the steps of 1 preparation before semen collection, 2 bare-handed semen collection, 3 sperm motility rate and density inspection judgment and 4 artificial insemination. 3-8 pigs can be hybridized through one semen collection of one improved-variety boar, 300-1000 pigs can be hybridized within one year, the number of the hybridized pigs is 3-7 times that of pigs obtained through natural mating, the number of bred boars is greatly decreased, the cultivation cost is lowered, and the method is high in conception rate, high in birth rate and remarkable in economic benefit.

The invention discloses a vector and method for increasing cattle cloning efficiency based on histone methylation level modification. The vector comprises the in-vitro transcription template vector containing a cattle KDM4E gene. A large amount of mRNA of the cattle KDM4E gene can be obtained by subjecting the vector to in-vitro transcription reaction, and the mRNA is injected to a successfully fused and activated bovine somatic cell cloning embryo to allow the embryo to overexpress cattle KDM4E protein. The method has the advantages that histone H3K9me3 and H3K9me2 epigenetic modification level of the somatic cell cloning embryo during zygotic activation can be effectively lowered, so that the development rate and quality of later bovine somatic cell cloning embryo can be increased evidently, the method can be used for efficiently producing the bovine somatic cell cloning embryo in vitro, and the birth rate of cloning cattle after embryo transplanting is increased evidently.

The invention relates to a nucleic acid composition and a detection kit for detecting genetic anemia as well as a use method. The nucleic acid composition and the detection kit for detecting the genetic anemia can be used for simultaneously detecting four deletion type alpha thalassemia genes, three non-deletion type alpha thalassemia genes, nineteenth mutation type beta thalassemia genes, geneticanemia types such as sickle-shaped thalassemia gene as well as specific gene mutation types. Compared with the prior similar technologies, the detection kit for the genetic anemia has the characteristics that several mutation detection types for the genetic anemia are increased, such as alphaTHAI deleted thalassemia, sickle-shaped thalassemia and 71 / 72(+T) mutation and -28M(A-C) mutation which are relatively-rare thalassemia types. The detection for the genetic anemia types can provide visual reference and prompt for clinically detecting the genetic anemia, so that the leak detection risk ofclinical genetic anemia can be greatly reduced and the birth rate of severe anemia children is reduced.

The invention discloses a phenylketonuria (PKU) screening kit and application thereof to prenatal screening, and belongs to the technical field of PKU screening. The application method comprises the following steps of: (1) extracting free fetal DNA (Deoxyribonucleic Acid) from peripheral blood of pregnant women; (2) taking the fetal DNA as a template to carry out polymerase chain reaction (PCR) amplification; (3) detecting a PCR product through agarose gel electrophoresis and purifying the PCR product through enzyme reaction; (4) carrying out sequencing reaction on the purified PCR product; (5) purifying a PCR sequencing reaction product; (6) sequencing the purified PCR sequencing reaction product; and (7) comparing the sequencing result with positive and negative control samples. The kit has the beneficial effects that the kit can achieve automatic sequencing, thus shortening the screening time and enabling PKU screening to be simpler, more convenient and faster; the technical gap of prenatal PKU screening is filled; the birth rate of sick children is reduced; and the kit causes no trauma to the fetuses and is safe and reliable.

The invention discloses a kit for external detection of Neurofibromastosis 2 disease causative gene NF2 c.1598delA mutation. The kit comprises a primer pair shown in the formulas of SEQ ID NO: 1 and SEQ ID NO: 2. The invention firstly discloses Neurofibromastosis 2 disease causative gene NF2 c.1598delA mutation and proves a relationship of the mutation site and the Neurofibromastosis 2 disease. The invention further designs the primers for detecting the mutation site and discloses a use of the primers in diagnosis of the Neurofibromastosis 2 disease. Through prenatal screening and neonatal NF2 gene mutation screening, a birth rate of the children suffering from the NF2 disease is reduced, disease is prevented and social pressure is greatly reduced.

The invention discloses a poultry micro-ecological preparation, relates to a feed micro-ecological preparation, and belongs to the field of feed. In view of the defects of single function and weak environmental adaptability to animal digestive tract of viable bacteria in existing micro-ecological preparation, the preparation provided by the invention has strong adaptability to animal digestive tract environment. The preparation is characterized by comprising, by weight, 45-60% of a Trichoderma-containing fertilizer, 0.5%-25% of lactobacillus delbrueckii, 12-15% of Bacillus subtilis, 15-18% of superphosphate, 18-20% of photosynthetic bacteria and 2-5% lactic acid bacteria. The preparation is beneficial to maintain micro-ecological balance of animal intestinal environment and can greatly improve animal immunity. The invention has beneficial effect that the preparation improves internal environment of livestock house, improves animal immunity, effectively prevents various diseases, and increases pregnancy rate, birth rate and survival rate.

The invention relates to a modeling and simulation method for a network artificial social model based on agent, and belongs to the technical field of computer simulation. The modeling and simulation method comprises the following steps: firstly, initializing a network, and establishing an acquaintance circle sub-network and a friend circle sub-network on a map of a sugarscape model based on a social circle theory; then, establishing populations of different levels based on the wealth amount, and dividing individuals in the space into three different levels according to the wealth amount; enabling the individuals to move and look for resources; enabling the individuals to carry out motion and wealth accumulation through motion and wealth acquiring manners; adding the birth rate, death rate and social mobile population; judging whether the individual wealth changes or not, and simulating the network evolution process through computation of structure parameters of the network; finally, modeling and simulating wealth variation of the social populations. The modeling and simulation method provides a reference model for relieving the serious problem of social polarization between the rich and the poor, and provides a preliminary theoretical basis for governments to take measures for relieving the polarization between the rich and the poor.

The present invention relates to a method for super-conventional breeding of fine-bred dairy cows using embryonic biotechnology, selecting excellent and suitable cows as donors, the method comprising the following steps: a, superovulation, b, artificial insemination c, embryo punching, d , Embryo inspection, e, embryo freezing, f, embryo thawing, g, embryo separation, h, embryo transfer. By adopting the above method, the birth rate of female calves can be effectively controlled, the production cost is reduced, the quality of dairy cows and milk is improved, and the purpose of supernormal rapid breeding of female dairy cows is achieved.

The invention relates to an agent social circle network based modeling simulation method for a universal multi-language competition model, belonging to the field of computer simulation. According to the method, the number of agents in the social circle is set, and a population proportion of a single language, status of three groups of languages, small, medium and large social radiuses, individual migration proportion, birth rate and death rate are set; agents establish a social network by use of a social circle principle according to respectivesocial radius; in the established network, individuals perform language communication through learning and forgetting, and children inherit the language from parents; the dynamism of the social network is depicted according to introduced individual migration proportion, birth rate and death rate; and modeling and simulation are performed on language competition spread by use of the established social circle network. According to the method, characteristics of the dynamic language spread network can be represented correctly, social network based quantitative explanation of multi-language evolution can be offered, and the model frame provides a feasible new method for further research of multi-language competition.

The invention discloses a composition and a method for improving recombination efficiency of transgenic cells. Through transgenic cells processed by adopting a culture medium containing SCR7, RS-1 orL755507, the HDR efficiency of the transgenic cells is improved. According to some embodiment of the invention, through using specific sgRNA combination, the higher transgenic efficiency is achieved,and the defects that a fertilized ovum is damaged more seriously because of secondary injection and the positive reproductive number is insufficient because the birth rate is dropped more seriously are solved.

The invention discloses a panda population prediction method based on a multi-environmental membrane system. The panda population prediction method comprises a processor, wherein the output end of theprocessor is electrically connected with a configuration file; the output end of the processor is electrically connected with a script file; the output end of the script file is electrically connected with a memory; and the output end of the memory is electrically connected with a robustness testing device and a sensitivity testing device. The panda population prediction method can perform symbolization on the evolutionary behavior of a concept model by inputting original pedigree data of pandas of two bases of GPBB and CCRCGP, designing the concept model of a multi-environment membrane system, and designing a calculation model of the multi-environment membrane system, selects a MeCoSim software platform to carry out panda population prediction simulation on the multi-environment membranesystem of the calculation model, takes core parameters such as original panda data and the statistical birth rate as initial data, selects a parameter uncertainty probability distribution method to predict the combined action of probability parameter values of different panda individual age groups, and is convenient for completing work of predicting the population number according to species evolution behaviors.

The invention discloses a method for improving mammal cloning efficiency based on acquisition of specific donor cells. According to the method, the donor cells with low-level DNA (deoxyribonucleic acid) methylation in XIST-DMR (X-inactive specific transcript-deuteron magnetic resonance) are acquired and used for nuclear transfer experiments, so that the mammal cloning efficiency is improved. Cloned embryos generated by the method for improving the mammal cloning efficiency based on acquisition of the specific donor cells are lower in XIST-DMR DNA methylation level at a blastula stage, so thatthe development birth rate of the cloned embryos is remarkably increased.

The invention provides a hasten parturition method for artificially bred giant salamanders. The hasten parturition method comprises the following steps of establishing a small outdoor ecology-imitating breeding place according to the local climate of the giant salamander breeding place and natural conditions of water temperature and water quality; and promoting estrus on giant salamander parents in the corresponding breeding place according to conditions of spawning of the giant salamanders in the outdoor ecology-imitating breeding place, and enabling the giant salamanders to spawn. According to the hasten parturition method, the human and the nature are combined, an ecology imitation principle is utilized, the estrus promotion time of the giant salamanders in a year is accurately controlled by observation, the birth rate of the giant salamanders in the current year is increased by about 13%-17%, so that the yield is improved, the cost is reduced, and the income of farmers is increased.

The invention discloses a biological preventing and controlling method for eliminating aphids and belongs to the technical field of biological prevention and control. The method comprises the steps of radish planting, aphid inoculating reproduction, diaeretiella rapae inoculating reproduction, management, disinfection and purification and rejuvenation in the breeding process of the aphids and the diaeretiella rapae and large-scale breeding. The biological preventing and controlling method has the advantages that radish with large blade space and small humidity serves as a host plant material for hosting the aphids, mildew infection in the breeding process of the breeding aphids and the breeding diaeretiella rapae is avoided, and when the aphids and the diaeretiella rapae are inoculated, operation is convenient and flexible, and management is easy; the bred aphids and the bred diaeretiella rapae are strong and large, the migratory flight rate of the aphids is low, and the birth rate of the aphids and the diaeretiella rapae is high; it can be guaranteed that the number and the quality of the breeding aphids and the breeding diaeretiella rapae are high; according to the biological preventing and controlling method for eliminating the aphids, the production cost is low, operation is easy and convenient, safety is achieved, no viral infection is caused, and large-scale breeding and popularization of the diaeretiella rapae can be achieved conveniently.

The present invention relates to methods and formulations directed to increasing mammalian birth rates comprising the administration of processed Morinda citrifolia based formulations.

The invention relates to a method for unconventionally breeding improved cows through an embryo biotechnology. Excellent and suitable female cows are selected as donors. The method comprises the following steps of (1) superovulation, (2) artificial insemination, (3) embryo washing, (4) embryo checking, (5) embryo freezing, (6) embryo unfreezing, (7) embryo separation, and (8) embryo transplantation. By the adoption of the method, the birth rate of female calves can be effectively controlled, production cost is reduced, quality of the cows and milk is improved, and the purpose of unconventionally and rapidly breeding the cows is achieved.

The invention relates to a health-care food, and in particular discloses pregnancy full-nutrition eugenics paste. The pregnancy full-nutrition eugenics paste is prepared from the following raw materials in certain parts by weight: gorgon euryale seed, red peanut, soybean, red kidney bean, chickpea, Chinese chestnut, lotus seed, longan pulp, black sesame, date, walnut kernel, yellow rice, red rice, millet, sorghum rice, selenium-rich rice, and black glutinous rice. The pregnancy full-nutrition eugenics paste has the advantages that after eating for a long time, the nutrition level of a pregnant woman is favorably improved, the normal development of a fetus is guaranteed, the complication of the pregnant woman is reduced, the condition of low-weight children and the birth rate of deformed children are decreased, and the quality of population is favorably improved; the preparation is simple and convenient, the mouth feel is fragrant and sweet, the digestion and absorption are easy, and the pregnancy full-nutrition eugenics paste is suitable for the growth and development of pregnancy fetuses; after the pregnant woman eats the pregnancy full-nutrition eugenics paste, the body immunity is improved, the sleeping quality is improved, the appetite is increased, and a foundation is laid for the prenatal and postnatal care.